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31 results on '"Houterman, Petra M."'

6. Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium

Author

Ma, Li-Jun, van der Does, Charlotte H., Borkovich, Katherine A., Coleman, Jeffrey J., Daboussi, Marie-Josée, Di Pietro, Antonio, Dufresne, Marie, Freitag, Michael, Grabherr, Manfred, Henrissat, Bernard, Houterman, Petra M., Kang, Seogchan, Shim, Won-Bo, Woloshuk, Charles, Xie, Xiaohui, Xu, Jin-Rong, Antoniw, John, Baker, Scott E., Bluhm, Burton H., Breakspear, Andrew, Brown, Daren W., Butchko, Robert A. E., Chapman, Sinead, Coulson, Richard, Coutinho, Pedro M., Danchin, Etienne G. J., Diener, Andrew, Gale, Liane R., Gardiner, Donald M., Goff, Stephen, Hammond-Kosack, Kim E., Hilburn, Karen, Hua-Van, Aurélie, Jonkers, Wilfried, Kazan, Kemal, Kodira, Chinnappa D., Koehrsen, Michael, Kumar, Lokesh, Lee, Yong-Hwan, Li, Liande, Manners, John M., Miranda-Saavedra, Diego, Mukherjee, Mala, Park, Gyungsoon, Park, Jongsun, Park, Sook-Young, Proctor, Robert H., Regev, Aviv, Ruiz-Roldan, Carmen M., Sain, Divya, Sakthikumar, Sharadha, Sykes, Sean, Schwartz, David C., Turgeon, Gillian B., Wapinski, Ilan, Yoder, Olen, Young, Sarah, Zeng, Qiandong, Zhou, Shiguo, Galagan, James, Cuomo, Christina A., Kistler, Corby H., and Rep, Martijn

Published
2010
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12. Comparative genomics of geographically distant Fusarium fujikuroi isolates revealed two distinct pathotypes correlating with secondary metabolite profiles

Author

Niehaus, Eva-Maria, primary, Kim, Hee-Kyoung, additional, Münsterkötter, Martin, additional, Janevska, Slavica, additional, Arndt, Birgit, additional, Kalinina, Svetlana A., additional, Houterman, Petra M., additional, Ahn, Il-Pyung, additional, Alberti, Ilaria, additional, Tonti, Stefano, additional, Kim, Da-Woon, additional, Sieber, Christian M. K., additional, Humpf, Hans-Ulrich, additional, Yun, Sung-Hwan, additional, Güldener, Ulrich, additional, and Tudzynski, Bettina, additional

Published
2017
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14. A mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species

Author

van Dam, Peter, Fokkens, Like, Ayukawa, Yu, van der Gragt, Michelle, ter Horst, Anneliek, Brankovics, Balazs, Houterman, Petra M., Arie, Tsutomu, Rep, Martijn, van Dam, Peter, Fokkens, Like, Ayukawa, Yu, van der Gragt, Michelle, ter Horst, Anneliek, Brankovics, Balazs, Houterman, Petra M., Arie, Tsutomu, and Rep, Martijn

Abstract

The genome of Fusarium oxysporum (Fo) consists of a set of eleven 'core' chromosomes, shared by most strains and responsible for housekeeping, and one or several accessory chromosomes. We sequenced a strain of Fo f.sp. radicis-cucumerinum (Forc) using PacBio SMRT sequencing. All but one of the core chromosomes were assembled into single contigs, and a chromosome that shows all the hallmarks of a pathogenicity chromosome comprised two contigs. A central part of this chromosome contains all identified candidate effector genes, including homologs of SIX6, SIX9, SIX11 and SIX 13. We show that SIX6 contributes to virulence of Forc. Through horizontal chromosome transfer (HCT) to a non-pathogenic strain, we also show that the accessory chromosome containing the SIX gene homologs is indeed a pathogenicity chromosome for cucurbit infection. Conversely, complete loss of virulence was observed in Forc016 strains that lost this chromosome. We conclude that also a non-wilt-inducing Fo pathogen relies on effector proteins for successful infection and that the Forc pathogenicity chromosome contains all the information necessary for causing root rot of cucurbits. Three out of nine HCT strains investigated have undergone large-scale chromosome alterations, reflecting the remarkable plasticity of Fo genomes.

Published
2017

16. The effector repertoire of Fusarium oxysporum determines the tomato xylem proteome composition following infection

Author

Gawehns, Fleur, Ma, Lisong, Bruning, Oskar, Houterman, Petra M., Boeren, Sjef, Cornelissen, B.J.C., Rep, Martijn, Takken, Frank L.W., Gawehns, Fleur, Ma, Lisong, Bruning, Oskar, Houterman, Petra M., Boeren, Sjef, Cornelissen, B.J.C., Rep, Martijn, and Takken, Frank L.W.

Abstract

Plant pathogens secrete small proteins, of which some are effectors that promote infection. During colonization of the tomato xylem vessels the fungus Fusarium oxysporum f.sp. lycopersici (Fol) secretes small proteins that are referred to as SIX (Secreted In Xylem) proteins. Of these, Six1 (Avr3), Six3 (Avr2), Six5, and Six6 are required for full virulence, denoting them as effectors. To investigate their activities in the plant, the xylem sap proteome of plants inoculated with Fol wild-type or either AVR2, AVR3, SIX2, SIX5, or SIX6 knockout strains was analyzed with nano-Liquid Chromatography-Mass Spectrometry (nLC-MSMS). Compared to mock-inoculated sap 12 additional plant proteins appeared while 45 proteins were no longer detectable in the xylem sap of Fol-infected plants. Of the 285 proteins found in both uninfected and infected plants the abundance of 258 proteins changed significantly following infection. The xylem sap proteome of plants infected with four Fol effector knockout strains differed significantly from plants infected with wild-type Fol, while that of the SIX2-knockout inoculated plants remained unchanged. Besides an altered abundance of a core set of 24 differentially accumulated proteins (DAPs), each of the four effector knockout strains affected specifically the abundance of a subset of DAPs. Hence, Fol effectors have both unique and shared effects on the composition of the tomato xylem sap proteome.

Published
2015

18. TheAVR 2– SIX 5gene pair is required to activate I‐2 ‐mediated immunity in tomato

Author

Ma, Lisong, primary, Houterman, Petra M., additional, Gawehns, Fleur, additional, Cao, Lingxue, additional, Sillo, Fabiano, additional, Richter, Hanna, additional, Clavijo‐Ortiz, Myriam J., additional, Schmidt, Sarah M., additional, Boeren, Sjef, additional, Vervoort, Jacques, additional, Cornelissen, Ben J. C., additional, Rep, Martijn, additional, and Takken, Frank L. W., additional

Published
2015
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25. The AVR2- SIX5 gene pair is required to activate I-2-mediated immunity in tomato.

Author

Ma, Lisong, Houterman, Petra M., Gawehns, Fleur, Cao, Lingxue, Sillo, Fabiano, Richter, Hanna, Clavijo‐Ortiz, Myriam J., Schmidt, Sarah M., Boeren, Sjef, Vervoort, Jacques, Cornelissen, Ben J. C., Rep, Martijn, and Takken, Frank L. W.

Subjects
*DISEASE resistance of plants, *EPITOPES, *GENE expression in plants, *PLANT micropropagation, TOMATO genetics
Abstract

Plant-invading microbes betray their presence to a plant by exposure of antigenic molecules such as small, secreted proteins called 'effectors'. In Fusarium oxysporum f. sp . lycopersici ( Fol) we identified a pair of effector gene candidates, AVR2- SIX5, whose expression is controlled by a shared promoter., The pathogenicity of AVR2 and SIX5 Fol knockouts was assessed on susceptible and resistant tomato ( Solanum lycopersicum) plants carrying I-2. The I-2 NB- LRR protein confers resistance to Fol races carrying AVR2., Like Avr2, Six5 was found to be required for full virulence on susceptible plants. Unexpectedly, each knockout could breach I-2-mediated disease resistance. So whereas Avr2 is sufficient to induce I-2-mediated cell death, Avr2 and Six5 are both required for resistance. Avr2 and Six5 interact in yeast two-hybrid assays as well as in planta. Six5 and Avr2 accumulate in xylem sap of plants infected with the reciprocal knockouts, showing that lack of I-2 activation is not due to a lack of Avr2 accumulation in the SIX5 mutant., The effector repertoire of a pathogen determines its host specificity and its ability to manipulate plant immunity. Our findings challenge an oversimplified interpretation of the gene-for-gene model by showing requirement of two fungal genes for immunity conferred by one resistance gene. [ABSTRACT FROM AUTHOR]

Published
2015
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31. Effector gene screening allows unambiguous identification of Fusarium oxysporum f. sp. lycopersici races and discrimination from other formae speciales.

Author

Lievens, Bart, Houterman, Petra M., and Rep, Martijn

Subjects
*GENETIC testing, *FUSARIUM oxysporum, *TOMATO diseases & pests, *FUNGI, *MYCOSES, *PROTEINS, *XYLEM, *VASCULAR system of plants, *GENES
Abstract

During infection of tomato, the fungus Fusarium oxysporum f. sp. lycopersici secretes several unique proteins, called ‘secreted in xylem’ (Six) proteins, into the xylem sap. At least some of these proteins promote virulence towards tomato and among them, all predicted avirulence proteins that can trigger disease resistance in tomato have been found. In this study, a large, worldwide collection of F. oxysporum isolates was screened for the presence of seven SIX genes ( SIX1– SIX7). The results convincingly show that identification of F. oxysporum formae speciales and races based on host-specific virulence genes can be very robust. SIX1, SIX2, SIX3 and SIX5 can be used for unambiguous identification of the forma specialis lycopersici. In addition, SIX4 can be used for the identification of race 1 strains, while polymorphisms in SIX3 can be exploited to differentiate race 2 from race 3 strains. For SIX6 and SIX7, close homologs were found in a few other formae speciales, suggesting that these genes may play a more general role in pathogenicity. Host specificity may be determined by the unique SIX genes, possibly in combination with the absence of genes that trigger resistance in the host. [ABSTRACT FROM AUTHOR]

Published
2009
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