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Single pores in the resistive-pulse technique are used as an analytics tool to detect, size, and characterize physical as well as chemical properties of individual objects such as molecules and particles. Each object passing through a pore causes a transient change of the transmembrane current called a resistive pulse. In high salt concentrations when the pore diameter is significantly larger than the screening Debye length, it is assumed that the particle size and surface charge can be determined independently from the same experiment. In this article we challenge this assumption and show that highly charged hard spheres can cause a significant increase of the resistive-pulse amplitude compared to neutral particles of a similar diameter. As a result, resistive pulses overestimate the size of charged particles by even 20%. The observation is explained by the effect of concentration polarization created across particles in a pore, revealed by numerical modeling of ionic concentrations, ion current, and local electric fields. It is notable that in resistive-pulse experiments with cylindrical pores, concentration polarization was previously shown to influence ionic concentrations only at pore entrances; consequently, additional and transient modulation of resistive pulses was observed when a particle entered or left the pore. Here we postulate that concentration polarization can occur across transported particles at any particle position along the pore axis and affect the magnitude of the entire resistive pulse. Consequently, the recorded resistive pulses of highly charged particles reflect not only the particles' volume but also the size of the depletion zone created in front of the moving particle. Moreover, the modeling identified that the effective surface charge density of particles depended not only on the density of functional groups on the particle but also on the capacitance of the Stern layer. The findings are of crucial importance for sizing particles and characterizing their surface charge properties.

Recently, nanofluidic osmotic power, a promising technology converting the salinity difference between brine and fresh water into electricity using nanopores, has drawn the attention of researchers. Previous studies in this field were based mainly on nanopores having a smooth inner surface. To enhance the performance of nanofluidic osmotic power, we investigated four types of cylindrical nanopores, each with a unique waveform wall design (square, saw-tooth, triangle, and sine waves). This study focused on elucidating the influence of bulk salt concentration and geometric characteristics at the solid–liquid interface. We demonstrated that the presence of a waveform wall introduces new variables that have a significant impact on the overall performance of a nanofluidic osmotic power system. At the optimal amplitude of the waveform wall, raising waveform frequency can remarkably improve the osmotic current, diffusion potential, maximum power, and maximum efficiency. The present study provides a novel aspect of osmotic power, where the geometric nature of the nanopore reveals profound and intriguing phenomena primarily attributed to the distribution of ions within its interior. [ABSTRACT FROM AUTHOR]