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187 results on '"Hu, Qiongbo"'

13. Additional file 3 of Binding proteins of destruxin A from Metarhizium against insect cell

Author

Wang, Jingjing, Weng, Qunfang, Zhang, Ke, and Hu, Qiongbo

Abstract

Additional file 3: Figure S1. Protein interaction analysis by STRING database (left) and KEGG pathway clustering analysis (right) in cell lysis group candidate proteins. Figure S2. Protein interaction analysis by STRING database (left) and KEGG pathway clustering analysis (right) in live cell group candidate proteins. Figure S3. Expression and purification of candidate protein. Figure S4. Detailed SPR results of interaction of DA with candidate proteins.

Published
2023
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15. Molecular Mechanism of Male Sterility Induced by 60 Co γ-Rays on Plutella xylostella (Linnaeus).

Author

Li, Shifan, Zhang, Ke, Wen, Jiaqi, Zeng, Yuhao, Deng, Yukun, Hu, Qiongbo, and Weng, Qunfang

Subjects
DIAMONDBACK moth, SPERMATOZOA, AMINO acid transport, MALE sterility in plants, AMINO acid metabolism, RADIATION damage, BRASSICACEAE, SPERM motility
Abstract

Plutella xylostella (Linnaeus) is one of the notorious pests causing substantial loses to numerous cruciferous vegetables across many nations. The sterile insect technique (SIT) is a safe and effective pest control method, which does not pollute the environment and does not produce drug resistance. We used proteomics technology and bioinformatics analysis to investigate the molecular mechanisms responsible for the effects of different doses of radiation treatment on the reproductive ability of male P. xylostella. A total of 606 differentially expressed proteins (DEPs) were identified in the 200 Gy/CK group, 1843 DEPs were identified in the 400 Gy/CK group, and 2057 DEPs were identified in the 400 Gy/200 Gy group. The results showed that after 200 Gy irradiation, the testes resisted radiation damage by increasing energy supply, amino acid metabolism and transport, and protein synthesis, while transcription-related pathways were inhibited. After 400 Gy irradiation, the mitochondria and DNA in the testis tissue of P. xylostella were damaged, which caused cell autophagy and apoptosis, affected the normal life activities of sperm cells, and greatly weakened sperm motility and insemination ability. Meanwhile, Western blotting showed that irradiation affects tyrosine phosphorylation levels, which gradually decrease with increasing irradiation dose. [ABSTRACT FROM AUTHOR]

Published
2023
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21. Effects of 60Co-γ radiation on testis physiological aspects of Plutella xylostella (Linnaeus)

Author

Ke Zhang, Guohua Zhong, Hu Qiongbo, He Renkun, Deng Yukun, Xiaofeng Zhang, Weng Qunfang, and Xiaoxue Li

Subjects
Diamondback moth, γ radiation, biology, Spermatozoon, Cruciferous vegetables, Health, Toxicology and Mutagenesis, fungi, Public Health, Environmental and Occupational Health, Plutella, General Medicine, biology.organism_classification, Pollution, Sperm, Cell biology, Sterile insect technique, medicine.anatomical_structure, medicine
Abstract

The diamondback moth, Plutella xylostella (Linnaeus), is one of the notorious pests causing substantial loses to many cruciferous vegetables across the nations. Sterile insect technique (SIT) is considered as an effective bio-control agent for controlling numerous lepidopteran pests. We searched the deformity spermatozoon and sperm bundles of diamondback moth. In our research, 200 Gy and 400 Gy 60Co-γ radiation doesn’t alter the number of apyrene and eupyrene sperm bundles in testis. However, the ratio of abnormal eupyrene sperm bundles was increasing with radiation dosage. The malformation of mitochondrial derivatives is characterized by "V" shape with 400 Gy. Also, the results showed that the expression of caspase-3 with 200 Gy was down-regulated, but was obviously up-regulated after 400 Gy radiation. Thus the present research investigation highlights that the 60Co-γ radiation treatments alters the physiological development of diamondback moth testis.

Published
2019

30. The Effect of 200Gy 60Co-γ Radiation on the Body Stress Responses Sensitivity and Reproductive Reaction of Plutella xylostella (Linnaeus)

Author

Li, Xiaoxue, Luo, Lingyan, Karthi, Sengodan, Zhang, Ke, Luo, Jianjun, Hu, Qiongbo, and Weng, Qunfang

Subjects
fungi, other
Abstract

The diamondback moth, Plutella xylostella (Linnaeus), is one of the notorious pests causing substantial loses to many cruciferous vegetables across the nations. We have got the result that 200Gy dosage irradiated 6-day male pupae of P. xylostella inducing F1 generation sterile. First, in our research, we detected Oxidase system and stress response mechanism of irradiated pupae, the results displayed that 200Gy irradiation significantly alters the antioxidant enzyme regulation in 6-day male pupae of P. xylostella. The level of SOD, CAT were increased significantly in contrast the level of POD and GST were decreased in 12-24h post-treatment. The heat shock proteins (Hsps) gene expression level was significant increasing, maximum > 2 folds up-regulation of genes were observed in peak. But they also had a trend of gradual recovery with development. Second, in order to explore the irradiated sterility further, we detected the testis LDH and ACP activity found that in male adults testis they increased significantly than control during its development. Thus the present research investigation highlights that the 60Co-γ radiation treatments alters the physiological development of diamondback moth. The results showed that 200Gy dosage resulted stress damage to the body and reproductive system of the diamondback moth.

Published
2018

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