1. Preparation of Novel Restricted Access Material-Surface Molecularly Imprinted Polymers via Reversible Addition-Fragmentation Chain Transfer Polymerization for 17[beta]-Estradiol Determination in Milk Powder
- Author
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Zuo, Hai Gen, Liu, Xiao Yu, Du, Yong Qin, Xiong, Shi Yu, Hao, Ren Qing, Huang, Zhi Fang, and Hu, Mei Hua
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Estradiol -- Measurement ,Milk -- Contamination ,Polymerization -- Methods ,Dried milk -- Contamination ,Mass spectrometry -- Methods ,Polymers -- Usage ,Chemistry - Abstract
Novel surface molecularly imprinted polymers (MIPs) combined to restricted access materials (RAMs) were prepared by reversible addition-fragmentation chain transfer (RAFT) polymerization for selective and efficient determination of 17[beta]-estradiol (E2) in milk powder. TiO.sub.2 nanoparticles were coated by a MIP layer via RAFT polymerization and formed a core-shell structure. 3-Glycidoxypropyltrimethoxysilane was grafted on the surface of MIP and hydrolyzed to form hydroxyl group to block unspecific adsorption of macromolecules. RAM-MIPs were characterized by infrared, thermal analyzer, surface area and porosimeter analyzer, and others. RAM-MIP exhibited efficient adsorption performance for E2. E2 in milk powder was dissolved in water, cleaned up by RAM-MIP solid phase extraction cartridge, silanized by N-methyl-N-(trimethylsilyl)trifluoroacetamide, dithioerythritol, and trimethyliodosilane, and determined by gas chromatography-mass spectrometry. The limit of quantification for E2 in milk powder samples was 0.001 mg/kg. The recoveries and relative standard deviations of E2 spiked at 0.001-0.01 mg/kg were 82.0-107.5% and 2.9-5.8%, respectively. Thus, RAM-MIP can be successfully applied to the determination of E2 in milk powder samples., Author(s): Hai Gen Zuo [sup.1] , Xiao Yu Liu [sup.1] , Yong Qin Du [sup.1] , Shi Yu Xiong [sup.1] , Ren Qing Hao [sup.1] , Zhi Fang Huang [sup.1] [...]
- Published
- 2022
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