1. Oral immunization with recombinant Lactobacillus casei displayed AHA1-CK6 and VP2 induces protection against infectious pancreatic necrosis in rainbow trout (Oncorhynchus mykiss).
- Author
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Chen, Yaping, Hua, Xiaojing, Ren, Xuanyu, Duan, Kexin, Gao, Shuai, Sun, Jinhui, Feng, Ying, Zhou, Ying, Guan, Xin, Li, Dechuan, Wang, Na, Li, Jiahui, Yang, Jiawei, Xia, Dong, Shi, Wen, and Liu, Min
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LACTOBACILLUS casei , *RAINBOW trout , *ORAL vaccines , *MUCUS , *AEROMONAS hydrophila , *CHIMERIC proteins - Abstract
Infectious pancreatic necrosis virus (IPNV) primarily infects larvae and young salmonid with serious economic losses, which causes haemorrhage and putrescence of hepatopancreas. To develop a more effective oral vaccine against IPNV infection, the aeromonas hydrophila adhesion (AHA1) gene was used as a targeting molecule for intestinal epithelial cells. A genetically engineered Lactobacillus casei (pPG-612-AHA1-CK6-VP2/ L. casei 393) was constructed to express the AHA1-CK6-VP2 fusion protein. The expression of interest protein was confirmed by western blotting and the immunogenicity of pPG-612-AHA1-CK6-VP2/ L. casei 393 was evaluated. And the results showed that more pPG-612-AHA1-CK6-VP2/ L. casei 393 were found in the intestinal mucosal surface of the immunized group. The Lactobacillus -derived AHA1-CK6-VP2 fusion protein could induce the production of serum IgM and skin mucus IgT specific for IPNV with neutralizing activity in rainbow trouts. The levels of IL-1β , IL-8 and TNF-α isolated from the lymphocytes stimulated by AHA1-CK6-EGFP produced were significantly higher than EGFP group. For transcription levels of IL-1β , IL-8, CK6 , MHC-II , Mx and TNF-1α in the spleen, the result indicated that the adhesion and target chemokine recruit more immune cells to induce cellular immunity. The level of IPNV in the immunized group of pPG-612-AHA1-CK6-VP2/ L. casei 393 was significantly lower than that in the control groups. These data indicated that the adhesion and target chemokine could enhance antigen delivery efficiency, which provides a valuable strategy for the development of IPNV recombination Lactobacillus casei oral vaccine in the future. • The AHA1 and CK6 were co-expressed with VP2 of IPNV in expression system of Lactobacillus casei to prepare oral lactobacillus vaccine. • The L. casei 393 delivering the VP2 in combination with AHA1-CK6 can prolong the time of antigen in intestine and promote immune responses in rainbow trouts. • The genetically engineered pPG-612-AHA1-CK6-VP2/ L.casei 393 was able to effectively induce immune responses at both the systemic and mucosal levels. • The recombinant pPG-612-AHA1-CK6-VP2/ L.casei 393 is a promising candidate for the development of an oral vaccine against IPNV. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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