Search

Internal implants are widely used in most orthopedic surgeries, of which titanium and its alloys are most widely used owing to the excellent corrosiveness resistance, low elastic modulus and good biocompatibility. However, implant failure still occurs for that titanium and its alloys themselves do not own antibacterial and osteogenic properties. In this work, we successfully fabricated berberine-loaded graphene oxide (GO) on the surface of biomedical titanium and systematically investigated its capabilities of antibacteria and osteogenesis. In vitro results showed that berberine had low antibacterial activity, but GO loaded with berberine on titanium (Ber&GO@Ti) exhibited superior antibacterial activity against Staphylococcus aureus (S. aureus) with the synergistic effect of GO and berberine. Meanwhile, Ber&GO@Ti performed satisfactory cytocompatibility and was capable of promoting osteogenic differentiation of MC3T3-E1 cells. In the vivo experiment, Ber&GO@Ti showed excellent antibacterial properties and inflammatory cells e.g., neutrophils had seldom been found. No visceral toxicity had been found. This multifunctional coating showed great potential in orthopedic implants.

A simple method for preparing water-dispersable multifunctional germanium nanoparticles (GeNPs) in chitosan (CS) colloid was reported, which met the growing interest of green chemistry. The monodisperse CS–GeNPs were synthesized through the formation of Ge–N bond with a mean particle size of 60 nm and were characterized by UV–visible absorption spectroscopy, FTIR, HR-TEM and XRD measurements. The size and dispersibility of CS–GeNPs were controlled through tuning the initial dosage of CS. To test its potential in biological applications, CS–GeNPs were functionally loaded with doxorubicin (Dox) drug and conjugated with folic acid (FA), the resulting Dox-loaded FA–CS–GeNPs were capable of effectively killing MCF-7 human cancer cells. Fluorescent images showed that Dox-loaded FA–CS–GeNPs targeted cancer cells through FA receptors-mediated pathway. Furthermore, GeNPs had high efficiency of photothermal conversion. This study showed a new type of multifunctional Ge nanoparticles to achieve synergetic effect between drug-targeted delivery and photothermal therapy.

A novel poly dopamine-hyaluronic acid (PDA-HA) electrochemical sensor was successfully constructed based on electrostatic interactions. Electrochemical impedance method was used to detect H2O2 based on the degradation processes between hyaluronic acid and hydrogen peroxide. The results showed that the sensor could be successfully applied to the detection of H2O2 in 0.01 M PBS (pH 7.0) medium after reaction for 30–40 min. The linear range was 1.0 × 10−5 −4.0 × 10−4 M and the detection limit was 2.0 × 10−6 M. The electrochemical sensor showed advantages of high sensitivity, low cost, satisfactory stability and reproducibility.

Esophageal cancer is mainly divided into squamous cell carcinoma and adenocarcinoma. Epidemiologically, the former contributes to 90% of worldwide esophageal cancer cases, while adenocarcinoma contributes to two-thirds of cases in developed countries. Although other rare types and collision with multiple histological types of tumors do occur in the esophagus, it is very rare for a gastrointestinal stromal tumor (GIST) to collide with an epithelial malignant tumor. To date, only three cases have been reported in the literature. The current study reported a 69-year-old male patient with squamous cell carcinoma and GIST in the middle esophagus. There was no merging of tissue components between these tumors. This study together with a literature review indicates that esophageal collision tumors have been increasingly reported in recent years. Histology and immunohistochemistry are needed to make a differential diagnosis. The exact oncogenic mechanism or the interaction of two independent neoplasms still remains to be determined, and further investigation, such as electron microscopy and genetic analysis, may help to elucidate the pathogenesis of the colliding tumors.

This study was aimed to analyze the contrast-enhanced CT features of gastrohepatic ligament (GHL) involvement in gastric carcinoma (GC) and to evaluate the influence of GHL on the spread of GC correlating with the anatomic bases.CT scans of 41 patients known to have GC and GHL involvement were reviewed retrospectively for the primary tumor and the GHL abnormalities, as well as the role GHL played in the spread of GC. Emphasis was placed on direct invasion, lymph node metastasis, and GHL seeding. The relationship between the accompanying ascites and the different pattern of the GHL involvement were also evaluated statistically.CT features of the GHL abnormalities caused by GC could be summarized as follows: (a) direct invasion (34.1%, 14 of 41), which was visualized as a regional (nine of 14) or diffuse mass (five of 14) in the GHL; (b) GHL seeding (26.8%, 11 of 41), which consists of ''smudged'' appearance (eight of 11), nodular infiltration (two of 11) and "GHL caking" (one of 11); (c) lymph node metastasis (63.4%, 26 of 41), including enlargement of lymph nodes (22 of 26) and cystic lesion (four of 26). We also found direct extension of GC into the transverse fissure and/or the liver via the GHL in three patients. Ascites, which was found in ten patients, seemed to be associated with the pattern of seeding involvement.GHL can be invaded by GC through several patterns and contrast-enhanced CT scan plays an important role in detecting GHL involvement in GC, which has a variety of CT manifestations. GHL may also serve as a potential conduit for the predictable spread of GC into the neighboring organs such as the liver.

The substrates removal performance, removal kinetics and the electron transport system (ETS) of sludge were investigated by sequencing batch reactors (SBR) and batch assays, respectively. Compared to the control system, significant decreases were observed in substrate removal efficiency with the Cr(VI)-feeding concentration up to 5 mg L(-1) in SBR system. And the recovery for NH4+-N removal were more difficult than that of COD after the termination of Cr(VI)-feeding. Significant inhibitory effects of Cr(VI) on the ETS activity and substrate removal kinetics were observed in the batch assays. The inhibitory effects of Cr(VI) would be overestimated on COD removal and underestimated on NH4+-N removal by the short-term batch assay as compared to the long-term operations. Additionally, significant correlations between the ETS activity and the inhibitory rates of Cr(VI) on substrate removal indicated the ETS activity can provide effective predictions on the potential performance of substrate removal in activated sludge.