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Aims Competition among plants in a community usually depends on their nitrogen (N)-use efficiency (NUE) and water-use efficiency (WUE) in arid and semi-arid regions. Artemisia frigida is an indicator species in heavily degraded grassland, however, how its NUE and WUE respond to N addition in different successional stages is still unclear, especially with mowing, a common management practice in semi-arid grasslands. Methods Based on a long-term controlled experiment with N addition and mowing in an abandoned cropland from 2006 to 2013, we investigated the NUE and WUE of A. frigida in two patches (i.e. grass and herb patches) in 2013 which represented two potential successional stages from herb to grass communities. The coverage of A. frigida was higher (about 50%) in the herb patch than in the grass patch (about 10%). Stable isotopic C (δ 13C) and N (δ 15N) as well as C and N pools were measured in plants and soils. NUE was calculated as leaf C/N, and leaf δ 13C values were used as a proxy for WUE. Important Findings N addition did not affect WUE of A. frigida, but significantly decreased NUE by 42.9% and 26.6% in grass and herb patches, respectively. The response of NUE to N addition was related to altering utilization of different N sources (NH4+vs. NO3−) by A. frigida according to the changed relationship between leaf δ 15N/soil δ 15N and NUE. Mowing had no effect on NUE regardless of N addition, but significantly increased WUE by 2.3% for A. frigida without N addition in the grass patch. The addition of N reduced the positive effect of mowing on its WUE in grass patch. Our results suggested that decreased NUE and/or WUE of A. frigida under mowing and N addition could reduce its competition, and further accelerate restoration succession from the abandoned cropland to natural grassland in the semi-arid region.

To explore the responses of soil net nitrogen (N) mineralization rate to precipitation varia-tion and nitrogen deposition in salinized grassland, we set precipitation manipulation and nitrogen addition experiments in the typical agro-pastoral ecotone saline-alkaline grassland of Northern Shanxi Province, China. The于2018年在晋北典型农牧交错带盐碱化草地设置增减降水和氮添加处理试验, 2019年生长季(5—9月)采用原位顶盖PVC埋管法测定不同水、氮处理下土壤净氮矿化速率,研究盐碱化草地土壤净氮矿化速率对降水格局变化和氮沉降的响应。结果表明: 土壤净氮矿化速率表现出明显的季节动态。单独增减降水(±50%)和氮添加(10 g·m

An experiment was conducted to investigate the effect of relative humidity (RH) at chronic temperature on growth performance, glucose consumption, and mitochondrial adenosine triphosphate (ATP) production of broilers. A total of 180 28-day-old Arbor Acres broilers (half males and half females) were randomly allocated to three treatments, each containing six replicates of 10 birds per treatment, using a completely randomized design. Birds were reared at 35, 60 or 85% RH at 32°C for 15 days (temperature increased by 3°C every 3 days from 20 to 32°C within 15 days: 20−23−26−29−32°C). RH affected (P

The responses of soil total respiration (Rs) and its components (soil autotrophic respiration (Ra) and heterotrophic respiration (Rh)) to nitrogen (N) deposition have been widely evaluated in non-saline-alkaline grasslands. However, their responses to N addition under drought and wet conditions, especially in saline-alkaline grasslands, remain unclear. A two-factorial experiment involving N addition and precipitation changes (decreased or increased 50% precipitation relative to ambient) was conducted in a saline-alkaline grassland of Northern China, while soil respiration was measured in different treatment plots during two growing seasons (2018, 2019). Results showed that the addition of N or changes in precipitation alone had no significant effect on Rs, Ra or Rh in both years because root productivity and soil microbial biomass were not affected. However, N addition with increased precipitation synergistically augmented seasonal mean Rs and Ra by 25.7% and 46.8% in 2018, and by 42.4% and 89.3% in 2019, respectively, owing to the increase in plant productivity. The variation in Ra primarily contributed to variation in Rs, and the effect size of N addition on Rs and Ra were increased with precipitation. In addition, a structural equation model showed that the response of soil respiration to N addition and precipitation changes was regulated by soil salinization, in which the Ra was regulated by soil based cations while Rh was controlled by soil pH values. Our study highlights that N addition with increased precipitation preferentially affects plants rather than microorganisms, and that Rh was not sensitive to N addition and precipitation changes in saline-alkaline grassland.

In order to measure the real-time variations in body temperature with increasing ambient temperature (AT) at different relative humidity (RH) levels, 60 Jinghong laying hens (35-wk-old) were raised in 3 controlled climate chambers (10 cages with 2 birds per chamber). The RH was fixed at one of 3 levels comprising 35, 50, or 85%, and the AT was increased gradually by 1 degree per 0.5 h from 18 to 35°C in the 3 chambers. The core temperature (CT) and surface temperature (ST) of the hens, as well as the AT in the 3 chambers were recorded at 3 min intervals using mini temperature data loggers. The data were analyzed with a broken-line model to determine the inflection point temperature (IPT, the certain AT above which the body temperature of the hens started to change). The experiment was repeated 3 times on 3 d. The IPTs of the laying hens were 23.89 and 25.46°C based on ST and CT at 50% RH, respectively, which indicated that the upper critical temperature of the thermoneutral zone of hens may be a specific temperature between 23.89°C and 25.46°C. The IPTs of the laying hens were 24.11 and 25.20°C based on ST and CT at RH 35%, respectively, and 21.93 and 24.45°C at RH 85%. The RH significantly affected the IPT of ST (P0.001). The IPTs were higher at 35 and 50% RH than that at 85% RH (P0.05). The coefficients of variation for the IPTs between individual hens were 2.96 to 4.51, and coefficients of variation for the IPTs for the same bird measured on 3 d were 0.69 to 1.59, thereby indicating that this method for estimating the IPTs of hens is stable and repeatable, although more samples are needed. In conclusion, our results indicate that analyzing the real-time variation in body temperature with increasing AT is a reliable method for estimating the IPT to provide an important reference for regulating the temperature in poultry houses.

The aim of the present study was to examine the effect of high temperature on the cecal microbiota of laying hens and analyze the correlation between variation in feed intake, body temperature, and the relative abundance of specific bacterial genera. Two hundred and sixteen 28-wk-old Hy-Line laying hens were randomly divided into 3 groups with 6 replicates and raised in 3 controlled climate chambers. Birds were allocated to either a thermoneutral group (TC, 21 ± 1°C, ad libitum), high cyclic temperature group (HT, 29-35°C, ad libitum), or a pair-feeding group (PF, 21 ± 1°C, pair-feeding on the daily feed intake of HT hens) for 4 wk. There was no significant difference in diversity estimators of laying hens between the HT and TC or PF groups (P > 0.05). Principal component analysis (PCoA) based on unweighted UniFrac distance showed that the HT group did not form a distinct cluster that was clearly separated from the PF group, but was separated from the TC group. Similarity analysis (ANOSIM) confirmed that the TC group was significantly different from the HT (R = 0.259, P = 0.020) and the PF (R = 0.348, P = 0.011) groups, but the HT group was similar with the PF group (R = 0.107, P = 0.153). In addition, there was a significant correlation between feed intake and principal coordinate 1 (PC1) in PCoA (R = -0.494, P = 0.037), but no correlation between core temperature and PC1 (R = 0.025, P = 0.923). Linear discriminant analysis identified 15 genera that discriminated between the HT and TC groups (score ≥2). Among these, the feed intake was significantly related with the relative abundance of 10 genera, however, the core temperature was only significantly correlated with one specific genus. In conclusion, a high cyclic temperature of 29-35°C had significant effects on microbial composition and structure of laying hens, which was mainly related with decreased feed intake.

Grazing is one of the most important ways for managing grassland in northern China. Different studies have focused on the effects of grazing on the structure and function of ecosystems. Grazing affects the structure and function of soil via biological and physical processes, such as animal trampling, feeding, and excretion, which further affects N

To determine the cardiac and renal arteriolar pathological changes in autopsied elderly hypertensive patients with left ventricular hypertrophy (LVH).Autopsy samples from 25 essential hypertension (EH) patients with LVH aged over 60 years and age-matched 8 controls were analyzed. LVH was further divided into three degrees from I to III according to left ventricular free wall thickness in EH patients. Quantitative measurements of arteriolar morphometric parameters in heart and kidney were performed under light microscope with computer image analysis post HE and Masson staining. The lesion index and plasma albumen infiltration of arteriole were evaluated by the semiquantitative method.The inner diameter (ID) and luminal cross-sectional area (LCSA) were significantly decreased while wall thickness (WT), wall cross-sectional area (WCSA), ratio of WCSA to LCSA (WCSA/LCSA) and ratio of WT to ID (WT/ID) were significantly increased in EH patients in proportion to LVH degree. Both cardiac and renal arterioles WCSA/LCSA and WT/ID were significantly decreased with increasing outer diameters (OD). Under the same OD rang, the pathological changes were more significant in the renal arterioles compared to those in the cardiac arterioles (P0.05). The arteriolar lesion index and the plasma albumen infiltration index of cardiac and renal arterioles in EH group were significantly higher than those in the control group (P0.01) and the arteriolar lesion index and the plasma albumen infiltration of arteriole in the renal tissue were significantly higher than those in the cardiac tissue (P0.01).Concentric remodeling occurs in the cardiac and renal arterioles of EH patients in proportion to LVH degree and renal arterioles lesions were significantly severer than that of cardiac arterioles in EH patients with LVH.