295 results on '"Huan, Jia"'
Search Results
2. Cytochrome c-peroxidase modulates ROS homeostasis to regulate the sexual mating of Sporisorium scitamineum
- Author
-
Enping Cai, Huan Jia, Ruqing Feng, Wenqiang Zheng, Lei Li, Li Zhang, Zide Jiang, and Changqing Chang
- Subjects
Sporisorium scitamineum ,cytochrome c-peroxidase ,reactive oxygen species ,pheromone response factor ,mating/filamentation ,Microbiology ,QR1-502 - Abstract
ABSTRACT Sugarcane smut, caused by the basidiomycetous fungus Sporisorium scitamineum, is a global fungal disease resulting in substantial economic losses. Our previous research has highlighted the significant role of reactive oxide species (ROS) in the sexual reproduction of S. scitamineum, yet the underlying mechanisms remain unclear. In this study, we identified the cytochrome c-peroxidase encoding gene, SsCCP1, as crucial for oxidative stress resistance. We further found that absence of SsCcp1 leads to defects in mating/filamentation and disrupts intracellular ROS homeostasis. However, restoration of mating/filamentation of SsCCP1 deletion mutants was partially achieved with exogenous antioxidants (vitamin C or vitamin E). Notably, transcription of the pheromone response factor SsPRF1, pivotal for mating/filamentation of S. scitamineum, is significantly reduced in the SsCCP1 deletion mutants or under exogenous hydrogen peroxide (H2O2) conditions. Nevertheless, the addition of exogenous vitamin C for 60 min significantly up-regulated the SsPRF1 gene in the SsCCP1 deletion mutants. Moreover, the constitutive expression of SsPRF1 essentially reinstated the mating/filamentation of SsCCP1 deletion mutants, suggesting that SsCcp1 mediates the transcriptional activity of SsPRF1 by maintaining ROS homeostasis to regulate the mating/filamentation of S. scitamineum. Further investigations revealed that SsCcp1 is necessary for ROS detoxification and full pathogenicity in planta. Overall, this study not only unveils the importance of SsCcp1 in S. scitamineum mating/filamentation, ROS detoxification, and virulence but also provides insights into the role of SsCcp1-regulated ROS homeostasis in sexual reproduction of S. scitamineum. IMPORTANCE Reactive oxygen species play an important role in pathogen-plant interactions. In fungi, cytochrome c-peroxidase maintains intracellular ROS homeostasis by utilizing H2O2 as an electron acceptor to oxidize ferrocytochrome c, thereby contributing to disease pathogenesis. In this study, our investigation reveals that the cytochrome c-peroxidase encoding gene, SsCCP1, not only plays a key role in resisting H2O2 toxicity but is also essential for the mating/filamentation and pathogenicity of S. scitamineum. We further uncover that SsCcp1 mediates the expression of SsPrf1 by maintaining intracellular ROS homeostasis to regulate S. scitamineum mating/filamentation. Our findings provide novel insights into how cytochrome c-peroxidase regulates sexual reproduction in phytopathogenic fungi, presenting a theoretical foundation for designing new disease control strategies.
- Published
- 2023
- Full Text
- View/download PDF
3. The biomedical knowledge graph of symptom phenotype in coronary artery plaque: machine learning-based analysis of real-world clinical data
- Author
-
Huan, Jia-Ming, Wang, Xiao-Jie, Li, Yuan, Zhang, Shi-Jun, Hu, Yuan-Long, and Li, Yun-Lun
- Published
- 2024
- Full Text
- View/download PDF
4. Towards a high-intensity muon source
- Author
-
Han-Jie Cai, Yuan He, Shuhui Liu, Huan Jia, Yuanshuai Qin, Zhijun Wang, Fengfeng Wang, Lixia Zhao, Neng Pu, Jianwei Niu, Liangwen Chen, Zhiyu Sun, Hongwei Zhao, and Wenlong Zhan
- Subjects
Nuclear and particle physics. Atomic energy. Radioactivity ,QC770-798 - Abstract
A high-intensity muon source driven by a continuous-wave superconducting linac holds the potential to significantly advance the intensity frontier of muon sources. Alongside advancements in accelerator technologies, breakthroughs in muon production target and collection schemes are essential. After a brief introduction to the development of the accelerator-driven system superconducting linac, a novel muon production target is proposed, utilizing a free-surface liquid lithium jet capable of handling the heat power generated by a proton beam with an energy of 600 MeV and a current of 5 mA. It is predicted by our simulation studies that the lithium target is more efficient in surface muon production compared to the rotating graphite target. The parameter space of the front end consisting of a lithium target and a large-aperture capture solenoid is explored, from the perspective of production efficiency, capture efficiency, and characteristics of the surface muon beam.
- Published
- 2024
- Full Text
- View/download PDF
5. Fixed and Sliding FBG Sensors-Based Triaxial Tip Force Sensing for Cable-Driven Continuum Robots.
- Author
-
Zecai Lin, Huanghua Liu, Xiaojie Ai, Weidong Chen 0001, Anzhu Gao, Zhenglong Sun 0001, Yun Zou, Guang-Zhong Yang, Hao Wu, and Huan Jia
- Published
- 2022
- Full Text
- View/download PDF
6. Thermal study of a scanning beam in granular flow target
- Author
-
Ping Lin, Yuanshuai Qin, Changwei Hao, Yuan Tian, Jiangfeng Wan, Huan Jia, Lei Yang, Wenshan Duan, Han-Jie Cai, and Sheng Zhang
- Subjects
Dense granular-flow target ,Scanning beam ,GPU ,Heat deposition ,Heat transfer ,Nuclear engineering. Atomic power ,TK9001-9401 - Abstract
The concept of dense granular-flow target (DGT) for the China Initiative Accelerator Driven Subcritical system (CiADS) is an attractive choice for high heat removal ability, low chemical toxicity, and radiotoxicity. A wobbling hollow beam is proposed to enhance the homogeneity of temperature rise of flowing particles in beam-target coupling zone. In this paper, the design procedure of target and beam parameters was discussed firstly. Then we simulated the heat deposition and transfer of the scanning beam in DGT to study the effect of beam parameters. The results show the flux density of proton beam plays a crucial role in the distribution of temperature rise while the contributions from scanning frequency heat transfer are also obvious. Moreover, heat transfer in transversal directions is insignificant, resulting in a low heat flux towards the sidewalls of DGT. This work not only contributes to the design of DGT, but also beneficial for understanding the beam-target coupling in porous materials.
- Published
- 2022
- Full Text
- View/download PDF
7. Beam-target configurations and robustness performance of the tungsten granular flow spallation target for an Accelerator-Driven Sub-critical system
- Author
-
Han-Jie Cai, Huan Jia, Xin Qi, Ping Lin, Sheng Zhang, Yuan Tian, Yuanshuai Qin, Xunchao Zhang, Lei Yang, and Yuan He
- Subjects
Granular flow target ,Accelerator-driven sub-critical system ,Beam-target configurations ,Accident conditions ,Robustness performance ,Nuclear engineering. Atomic power ,TK9001-9401 - Abstract
The dense granular flow spallation target is a new target concept proposed for an Accelerator-Driven Sub-critical (ADS) system. In this paper, the beam-target configurations of a tungsten granular flow target for the ADS with a thermal power of 1 GW is explored. The beam profile options using different scanning methods are discussed. The critical geometry parameters are adjusted to investigate the performance of the granular target from the aspects of neutron efficiency, stability and temperature distribution in target medium. To figure out how the target under accident conditions would behave, different clogging conditions are induced in the simulation. The dynamic processes are analyzed and some important parameters such as abnormal temperature rise and beam cutoff time window are obtained. The response of the sub-critical reactor to a clogging accident is also investigated. It is indicated that the monitoring of the granular flow by the neutron detectors in the sub-critical core will be effective.
- Published
- 2022
- Full Text
- View/download PDF
8. Preparation and Degradation Performance Study of P(AM/GG/PEGDA) Nanocomposite Self-Degradation Gel Plugging Material
- Author
-
Dan Bao, Siyuan Liu, Xianli Zhang, Feng Li, Jiaqin Wang, Huan Jia, Shanghao Liu, and Peng Zhang
- Subjects
reservoir ,drilling fluid lost circulation ,self-degradation plugging material ,gel ,degradation factor ,Science ,Chemistry ,QD1-999 ,Inorganic chemistry ,QD146-197 ,General. Including alchemy ,QD1-65 - Abstract
Lost circulation is a world-class problem, and the contradiction between plugging and unplugging in reservoirs is a problem that needs to be solved urgently. The traditional LCM is not suitable for reservoirs and the complex subsequent operations. Currently, a self-degrading plugging material is proposed. In this paper, a new self-degradation plugging material, CKS-DPPG, was prepared by AM, GG, nano silica, and PEGDA. The effects of reactant concentration, pH, mineralization, etc., on the swelling and degradation performance of CKS-DPPG were investigated. The plugging capacity was tested by fracture plugging equipment, and the mechanism of self-degradation was revealed. The results show that the CKS-DPPG reached a 50% degradation rate in 54 h and complete degradation in 106 h at 80 °C and pH = 8. Low temperatures, high mineralization, and weak alkaline conditions prolong the complete degradation time of CKS-DPPG, which facilitates subsequent operations. The simulation of the 3 mm opening fracture plugging experiment showed that the pressure-bearing capacity reached 6.85 MPa and that a 0.16 MPa pressure difference could unplug after degradation. The ester bond of PEGDA is hydrolyzed under high-temperature conditions, and the spatial three-dimensional structure of CKS-DPPG becomes linear. The CKS-DPPG can effectively reduce subsequent unplugging operations and lower production costs.
- Published
- 2023
- Full Text
- View/download PDF
9. A microscope-assisted endoscopic transcanal transpromontorial approach for vestibular schwannoma resection: a preliminary report
- Author
-
Jianqing, Chen, Yongchuan, Chai, Zhihua, Zhang, Huan, Jia, Zhaoyan, Wang, and Hao, Wu
- Published
- 2022
- Full Text
- View/download PDF
10. Study on Corrosion Resistance of Cationic Waterborne Acrylic Coating
- Author
-
ZHU Kang, FAN Wan-xin, HUAN Jia-qi, ZHANG Xiao-ya, SUN Ying, HU He-feng
- Subjects
waterborne anti-corrosive coating ,cationic hydroxy acrylic resin dispersion ,siloxane hydrolyzate ,tertiary amine groups ,corrosion resistance performance ,Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Technology - Abstract
Aiming at understanding the corrosion resistance of siloxane hydrolyzate cured cationic waterborne acrylic coatings, waterborne metal anti-corrosive coatings were prepared by cationic hydroxy acrylic resin dispersion containing tertiary amine group cured by siloxane hydrolysate WKHY-601.With using DSC, TGA, FT-IR and CHI660E electrochemical workstation, the curing mechanism of the coating and the main factors affecting its salt corrosion resistance were researched systematically.Results showed that the curing of the coating was achieved by the reaction of the silanol hydroxyl group and the alcohol hydroxyl group in the resin and the self-condensation of the siloxane hydrolyzate to form a cross-linked network.The content and type of the tertiary amine group in the resin and the amount of the curing agent siloxane hydrolyzate influenced the salt corrosion resistance of the coating film.As the content of tertiary amine groups increased, the self-corrosion potential of coating increased and the self-corrosion current density of coating declined.After reaching a certain level of the content of tertiary amine groups, the value remained stable.Besides, the dimethyl type tertiary amine group with less steric hindrance was better than the diethyl type, and the increase in the amount of siloxane hydrolyzate was beneficial to improve the compactness of the coating film and enhance its salt corrosion resistance.When the amount of siloxane hydrolyzate was excessive, the content of tertiary amine groups in the coating film would decrease, resulting in weakening the salt corrosion resistance.Furthermore, the self-corrosion potential of the coating sample prepared by DM type resin with a tertiary amine group content of 0.42 mmol/g and siloxane hydrolysate WKHY-601 according to a mass ratio of 3∶1 was as high as-0.298 V, and the self-corrosion current density was only 1.308×10-9 A/cm2, performing excellent salt corrosion resistance.
- Published
- 2022
- Full Text
- View/download PDF
11. Genetic findings of Sanger and nanopore single-molecule sequencing in patients with X-linked hearing loss and incomplete partition type III
- Author
-
Ying Chen, Jiajun Qiu, Yingwei Wu, Huan Jia, Yi Jiang, Mengda Jiang, Zhili Wang, Hai-Bin Sheng, Lingxiang Hu, Zhihua Zhang, Zhaoyan Wang, Yun Li, Zhiwu Huang, and Hao Wu
- Subjects
IP-III ,POU3F4 ,Nanopore single-molecule sequencing ,Hearing outcomes ,Medicine - Abstract
Abstract Background POU3F4 is the causative gene for X-linked deafness-2 (DFNX2), characterized by incomplete partition type III (IP-III) malformation of the inner ear. The purpose of this study was to investigate the clinical characteristics and molecular findings in IP-III patients by Sanger or nanopore single-molecule sequencing. Methods Diagnosis of IP-III was mainly based on clinical characteristics including radiological and audiological findings. Sanger sequencing of POU3F4 was carried out for these IP-III patients. For those patients with negative results for POU3F4 Sanger sequencing, nanopore long-read single-molecule sequencing was used to identify the possible pathogenic variants. Hearing intervention outcomes of hearing aids (HAs) fitting and cochlear implantation (CI) were also analyzed. Aided pure tone average (PTA) was further compared between two groups of patients according to their different locations of POU3F4 variants: in the exon region or in the upstream region. Results In total, 18 male patients from 14 unrelated families were diagnosed with IP-III. 10 variants were identified in POU3F4 by Sanger sequencing and 6 of these were reported for the first time (p.Gln181*, p.Val215Gly, p.Arg282Gln, p.Gln316*, c.903_912 delins TGCCA and p.Arg205del). Four different deletions that varied from 80 to 486 kb were identified 876–1503 kb upstream of POU3F4 by nanopore long-read single-molecule sequencing. De novo genetic mutations occurred in 21.4% (3/14) of patients with POU3F4 mutations. Among these 18 patients, 7 had bilateral HAs and 10 patients received unilateral CI. The mean aided PTA for HAs and CI users were 41.1 ± 5.18 and 40.3 ± 7.59 dB HL respectively. The mean PTAs for patients with the variants located in the exon and upstream regions were 39.6 ± 6.31 versus 43.0 ± 7.10 dB HL, which presented no significant difference (p = 0.342). Conclusions Among 14 unrelated IP-III patients, 28.6% (4/14) had no definite mutation in exon region of POU3F4. However, possible pathogenic deletions were identified in upstream region of this gene. De novo genetic mutations occurred in 21.4% (3/14) of patients with POU3F4 mutation. There was no significant difference of hearing intervention outcomes between the IP-III patients with variants located in the exon region and in the upstream region.
- Published
- 2022
- Full Text
- View/download PDF
12. Identification of Hypertension Subgroups through Topological Analysis of Symptom-Based Patient Similarity
- Author
-
Wang, Yi-fei, Wang, Jing-jing, Peng, Wei, Ren, Yong-hao, Gao, Chao, Li, Yun-lun, Wang, Rui, Wang, Xiao-feng, Han, Song-jun, Lyu, Jia-yu, Huan, Jia-ming, Chen, Cui, Wang, Hai-yan, Shu, Zi-xin, Zhou, Xue-zhong, and Li, Wei
- Published
- 2021
- Full Text
- View/download PDF
13. A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies
- Author
-
Patthara Kongsuphol, Huan Jia, Hoi Lok Cheng, Yue Gu, Bhuvaneshwari D/O Shunmuganathan, Ming Wei Chen, Sing Mei Lim, Say Yong Ng, Paul Ananth Tambyah, Haziq Nasir, Xiaohong Gao, Dousabel Tay, Seunghyeon Kim, Rashi Gupta, Xinlei Qian, Mary M. Kozma, Kiren Purushotorman, Megan E. McBee, Paul A. MacAry, Hadley D. Sikes, and Peter R. Preiser
- Subjects
Medicine - Abstract
Kongsuphol et al. develop a paper-based, vertical flow assay to detect SARS-CoV-2 neutralizing antibodies. The point-of-care assay has comparable performance to lab-based tests and provides results in 10 min.
- Published
- 2021
- Full Text
- View/download PDF
14. Rapid Evaluation of Vaccine Booster Effectiveness against SARS-CoV-2 Variants
- Author
-
Hoi Lok Cheng, Sing Mei Lim, Huan Jia, Ming Wei Chen, Say Yong Ng, Xiaohong Gao, Jyoti Somani, Sharmila Sengupta, Dousabel M. Y. Tay, Patrina W. L. Chua, Abirami R., Sharon Y. H. Ling, Megan E. McBee, Barnaby E. Young, Hadley D. Sikes, and Peter R. Preiser
- Subjects
COVID ,neutralizing antibodies ,point-of-care test ,Microbiology ,QR1-502 - Abstract
ABSTRACT As the COVID-19 pandemic continues, countries around the world are switching toward vaccinations and boosters to combat the pandemic. However, waning immunity against SARS-CoV-2 wild-type (WT) and variants have been widely reported. Booster vaccinations have shown to be able to increase immunological protection against new variants; however, the protection observed appears to decrease quickly over time suggesting a second booster shot may be appropriate. Moreover, heterogeneity and waning of the immune response at the individual level was observed suggesting a more personalized vaccination approach should be considered. To evaluate such a personalized strategy, it is important to have the ability to rapidly evaluate the level of neutralizing antibody (nAbs) response against variants at the individual level and ideally at a point of care setting. Here, we applied the recently developed cellulose pulled-down virus neutralization test (cpVNT) to rapidly assess individual nAb levels to WT and variants of concerns in response to booster vaccination. Our findings confirmed significant heterogeneity of nAb responses against a panel of SARS-CoV-2 variants, and indicated a strong increase in nAb response against variants of concern (VOCs) upon booster vaccination. For instance, the nAb response against current predominant omicron variant was observed with medians of 88.1% (n = 6, 95% CI = 73.2% to 96.2%) within 1-month postbooster and 70.7% (n = 22, 95% CI = 66.4% to 81.8%) 3 months postbooster. Our data show a point of care (POC) test focusing on nAb response levels against VOCs can guide decisions on the potential need for booster vaccinations at individual level. Importantly, it also suggests the current booster vaccines only give a transient protective response against some VOC and new more targeted formulations of a booster vaccine against specific VOC may need to be developed in the future. IMPORTANCE Vaccination against SARS-CoV-2 induces protection through production of neutralization antibodies (nAb). The level of nAb is a major indicator of immunity against SARS-CoV-2 infection. We developed a rapid point-of-care test that can monitor the nAb level from a drop of finger stick blood. Here, we have implemented the test to monitor individual nAb level against wild-type and variants of SARS-CoV-2 at various time points of vaccination, including post-second-dose vaccination and postbooster vaccination. Huge diversity of nAb levels were observed among individuals as well as increment in nAb levels especially against Omicron variant after booster vaccination. This study evaluated the performance of this point-of-care test for personalized nAb response tracking. It verifies the potential of using a rapid nAb test to guide future vaccination regimens at both the individual and population level.
- Published
- 2022
- Full Text
- View/download PDF
15. Application of Fourier transform near-infrared spectroscopy combined with GC in rapid and simultaneous determination of essential components in Amomum villosum
- Author
-
Guo, Huan-Jia, Weng, Wen-Feng, Zhao, Hong-Ning, Wen, Jin-Feng, Li, Rong, Li, Jun-Ni, Zeng, Chan-Biao, and Ji, Sheng-Guo
- Published
- 2021
- Full Text
- View/download PDF
16. Finger stick blood test to assess postvaccination SARS‐CoV‐2 neutralizing antibody response against variants
- Author
-
Sing Mei Lim, Hoi Lok Cheng, Huan Jia, Patthara Kongsuphol, Bhuvaneshwari D/O Shunmuganathan, Ming Wei Chen, Say Yong Ng, Xiaohong Gao, Shuvan Prashant Turaga, Sascha P. Heussler, Jyoti Somani, Sharmila Sengupta, Dousabel M. Y. Tay, Megan E. McBee, Barnaby E. Young, Paul A. MacAry, Hadley D. Sikes, and Peter R. Preiser
- Subjects
cellulose pulldown assay ,COVID19 ,humoral response against COVID19 variants ,neutralizing antibody ,point‐of‐care test ,SARS‐CoV‐2 ,Chemical engineering ,TP155-156 ,Biotechnology ,TP248.13-248.65 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Abstract There is clinical need for a quantifiable point‐of‐care (PoC) SARS‐CoV‐2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi‐quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccinated population against wild‐type (WT), alpha, beta, gamma, and delta variant RBDs. It captures a clinically relevant range of nAb levels, and effectively differentiates prevaccination, post first dose, and post second dose vaccination samples within 10 min. The data observed against alpha, beta, gamma, and delta variants agrees with published results evaluated in established serology tests. Finally, our test revealed a substantial reduction in nAb level for beta, gamma, and delta variants between early BNT162b2 vaccination group (within 3 months) and later vaccination group (post 3 months). This test is highly suited for PoC settings and provides an insightful nAb response in a postvaccinated population.
- Published
- 2022
- Full Text
- View/download PDF
17. The Study of Media Beta Elliptical Cavities for CADS
- Author
-
Liangjian, Wen, Shenghu, Zhang, Yongming, Li, Ruoxu, Wang, Hao, Guo, Cong, Zhang, Huan, Jia, Tiancai, Jiang, Chunlong, Li, and Yuan, He
- Subjects
Physics - Accelerator Physics - Abstract
The China Accelerator Driven Sub-critical System (CADS) is a high intensity proton facility to dispose of nuclear waste and generate electric power. CADS is based on 1.5GeV, 10mA CW superconducting (SC) linac as a driver. The high-energy section of the linac is compose of two families of SC elliptical cavities which are designed for the geometrical beta 0.63 and 0.82. In this paper, the 650 MHz \b{eta}=0.63 SC elliptical cavity was studied including cavity optimization, multipacting, high order modes (HOMs) and generator RF power calculation. Keywords: high current, medium beta, ADS, superconducting cavity, HOMs, Comment: 7 pages,10 figures
- Published
- 2015
- Full Text
- View/download PDF
18. Shielding Assessment and Optimization of the Target Station for Medical Isotope Production Based on Superconducting Proton Linac
- Author
-
Daiyuan Chen, Ricardo dos Santos Augusto, Yuanhong Li, Zhi Qin, Jian Rong, Kaiqiang Yao, Huan Jia, Chenzhang Yuan, Juntao Liu, and Zhiyi Liu
- Subjects
shielding ,dosimetry ,FLUKA ,medical isotope production ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
In response to the worldwide shortage of 99mTc supply caused by the combined consequence from both decommissioning and maintenance of research reactors worldwide, the Institute of Modern Physics (IMP) of the Chinese Academy of Science and Lanzhou University have launched a collaboration to research and develop a 99mTc production solution based on the 25 MeV high-intensity superconducting proton linear accelerator. Radiation from high-current proton bombardment must be evaluated and considered carefully at the design stage to meet radiation protection (RP) policy and requirements of the shielding for the key device. This work employed FLUKA to conduct the shielding assessment of both prompt and residual radiation fields in several iterations, based on a prototype of the high-power target system. The prompt dose rates outside the target station are lower than the institution’s limit. The residual dose rates inside the station fall below 100 μSv/h at 64 h after the end of beam (EOB); the dominant source term is then the target chamber. The service life of the main actuator is expected to be extended by 2.7 times with the current partial shielding design. The simulation accelerating techniques are applied to balance the accuracy of results and the progress of the project at the same time, which is referential to the shielding assessment of large-scale nuclear facilities. The results can also be used in further study and construction of the target station.
- Published
- 2023
- Full Text
- View/download PDF
19. Management of Laryngopharyngeal Reflux in Asia
- Author
-
Su Il Kim, Jerome R. Lechien, Tareck Ayad, Huan Jia, Seyyedeh Maryam Khoddami, Necati Enver, Sampath Kumar Raghunandhan, Abdul Latif Hamdan, and Young-Gyu Eun
- Subjects
laryngopharyngeal reflux ,otolaryngologist ,disease management ,therapeutics ,surveys and questionnaires ,Medicine ,Otorhinolaryngology ,RF1-547 - Abstract
Objectives. This study was conducted to investigate the current practices of Asian otolaryngologists for laryngopharyngeal reflux (LPR). Methods. An online survey about LPR was sent to 2,000 members of Asian otolaryngological societies, and a subgroup analysis was performed between Western and Eastern Asian otolaryngologists. The survey was conducted by the Laryngopharyngeal Reflux Study Group of Young Otolaryngologists of the International Federation of Oto-rhino-laryngological Societies. Results. Among approximately 1,600 Asian otolaryngologists, 146 completed the survey (62 from Western Asian countries, 84 from Eastern Asian countries). A substantial majority (73.3%) of the otolaryngologists considered LPR and gastroesophageal reflux disease to be different diseases. The symptoms thought to be closely related to LPR were coughing after lying down, throat clearing, and globus sensation. The findings thought to be closely related to LPR were posterior commissure granulations and hypertrophy, arytenoids, and laryngeal erythema. The respondents indicated that they mostly diagnosed LPR (70%) after an empirical therapeutic trial of proton pump inhibitors (PPIs). Although multichannel intraluminal impedance-pH (MII-pH) monitoring is a useful tool for diagnosing nonacid or mixed LPR, 78% of Asian otolaryngologists never or very rarely used MII-pH. Eastern Asian otolaryngologists more frequently used once-daily PPIs (64.3% vs. 45.2%, P=0.021), whereas Western Asian otolaryngologists preferred to use twice-daily PPIs (58.1% vs. 39.3%, P=0.025). The poor dietary habits of patients were considered to be the main reason for therapeutic failure by Asian otolaryngologists (53.8%). Only 48.6% of Asian otolaryngologists considered themselves to be adequately knowledgeable and skilled regarding LPR. Conclusion. Significant differences exist between Western and Eastern Asian otolaryngologists in the diagnosis and treatment of LPR. Future consensus statements are needed to establish diagnostic criteria and therapeutic regimens.
- Published
- 2020
- Full Text
- View/download PDF
20. Practical Calculation Method for the Short-Circuit Current of Power Grids with High Temperature Superconducting Fault Current Limiters
- Author
-
Hong, Hai-feng, Yu, Meng-ze, Zhang, Xiao-hui, Huan, Jia-jia, Sui, Yu, and Pan, Xian-xian
- Published
- 2020
- Full Text
- View/download PDF
21. Robot-Assisted Electrode Array Insertion Becomes Available in Pediatric Cochlear Implant Recipients: First Report and an Intra-Individual Study
- Author
-
Huan Jia, Jinxi Pan, Wenxi Gu, Haoyue Tan, Ying Chen, Zhihua Zhang, Mengda Jiang, Yun Li, Olivier Sterkers, and Hao Wu
- Subjects
cochlear implant ,robot-assisted ,robotic ,hearing loss ,scalar position ,Surgery ,RD1-811 - Abstract
Background: As an advanced surgical technique to reduce trauma to the inner ear, robot-assisted electrode array (EA) insertion has been applied in adult cochlear implantation (CI) and was approved as a safe surgical procedure that could result in better outcomes. As the mastoid and temporal bones are generally smaller in children, which would increase the difficulty for robot-assisted manipulation, the clinical application of these systems for CI in children has not been reported. Given that the pediatric candidate is the main population, we aim to investigate the safety and reliability of robot-assisted techniques in pediatric cochlear implantation.Methods: Retrospective cohort study at a referral center in Shanghai including all patients of simultaneous bilateral CI with robotic assistance on one side (RobOtol® system, Collin ORL, Bagneux, France), and manual insertion on the other (same brand of EA and CI in both side), from December 2019 to June 2020. The surgical outcomes, radiological measurements (EA positioning, EA insertion depth, mastoidectomy size), and audiological outcomes (Behavior pure-tone audiometry) were evaluated.Results: Five infants (17.8 ± 13.5 months, ranging from 10 to 42 months) and an adult (39 years old) were enrolled in this study. Both perimodiolar and lateral wall EAs were included. The robot-assisted EA insertion was successfully performed in all cases, although the surgical zone in infants was about half the size in adults, and no difference was observed in mastoidectomy size between robot-assisted and manual insertion sides (p = 0.219). The insertion depths of EA with two techniques were similar (P = 0.583). The robot-assisted technique showed no scalar deviation, but scalar deviation occurred for one manually inserted pre-curved EA (16%). Early auditory performance was similar to both techniques.Conclusion: Robot-assisted technique for EA insertion is approved to be used safely and reliably in children, which is possible and potential for better scalar positioning and might improve long-term auditory outcome. Standard mastoidectomy size was enough for robot-assisted technique. This first study marks the arrival of the era of robotic CI for all ages.
- Published
- 2021
- Full Text
- View/download PDF
22. Design and Development of the 200-kW Beam Dump
- Author
-
Huan Jia, Haihua Niu, Han-Jie Cai, Chenzhang Yuan, Xunchao Zhang, Yuanshuai Qin, Hongming Xie, Baifan Wang, Peng Zhang, Yuxuan Huang, Tieming Zhu, Tianji Peng, Weilong Chen, Qingwei Chu, Jianqiang Wu, Shenghu Zhang, Xiang Li, Duanyang Jia, Bin Zhang, Yuan He, Hongwei Zhao, and Wenlong Zhan
- Subjects
Nuclear Energy and Engineering - Published
- 2023
23. Thermal Safety of Endoscopic Usage in Robot-Assisted Middle Ear Surgery: An Experimental Study
- Author
-
Jinxi Pan, Haoyue Tan, Jun Shi, Zhaoyan Wang, Olivier Sterkers, Huan Jia, and Hao Wu
- Subjects
endoscopic ear surgery ,robot-assisted ,thermal damage ,endoscope ,robotic ,Surgery ,RD1-811 - Abstract
Objectives: The widespread application of endoscopic ear surgery (EES), performed through the external auditory canal, has revealed the limitations of the one-handed technique. The RobOtol® (Collin ORL, Bagneux, France) otological robotic system has been introduced to enable two-handed procedures; however, the thermal properties of dedicated endoscopes, which are usually used in neurosurgery, called “neuro-endoscopes,” have not yet been clarified for the robotic systems. In this study, we aimed to profile the thermal characteristics of two dedicated neuro-endoscopes, as compared to endoscopes used routinely in manual EES, called “oto-endoscopes,” and defined by a smaller diameter and shorter length, and to discuss the safe application of robotic assistance in EES.Methods: Two neuro-endoscopes (3.3 mm, 25 cm, 0°/30°) were studied using two routine light sources (LED/xenon), and two routine oto-endoscopes (3 mm, 14 cm, 0°/30°) were initially measured to provide a comprehensive comparison. Light intensities and temperatures were measured at different power settings. The thermal distributions were measured in an open environment and a human temporal bone model of EES. The cooling measures were also studied.Results: Light intensity was correlated with stabilized tip temperatures (P < 0.01, R2 = 0.8719). Under 100% xenon power, the stabilized temperatures at the tips of 0°, 30° neuro-endoscopes, and 0°, 30° oto-endoscopes were 96.1, 60.1, 67.8, and 56.4°C, respectively. With 100% LED power, the temperatures decreased by about 10°C, respectively. For the 0° neuro-endoscope, the illuminated area far away 1cm from the tip was below 37°C when using more than 50% both power, while this distance for 30° neuro-endoscope was 0.5 cm. In the EES temporal bone model, the round window area could reach 59.3°C with the 0° neuro-endoscope under 100% xenon power. Suction resulted in a ~1–2°C temperature drop, while a 10 mL saline rinse gave a baseline temperature which lasted for 2.5 min.Conclusion: Neuro-endoscope causes higher thermal releasing in the surgical cavity of ESS, which should be especially cautious in the robotic system usage. Applying submaximal light intensity, a LED source and intermittent rinsing should be considered for the safer robot-assisted EES using a neuro-endoscope that allows a two-handed surgical procedure.
- Published
- 2021
- Full Text
- View/download PDF
24. Low-dose IL-2 therapy limits the reduction in absolute numbers of circulating regulatory T cells in rheumatoid arthritis
- Author
-
Sheng-Xiao Zhang, Jia Wang, Cai-Hong Wang, Rui-Huan Jia, Ming Yan, Fang-Yuan Hu, Guang-Ying Liu, Xue-Yu Liu, Jing Luo, Chong Gao, and Xiao-Feng Li
- Subjects
Diseases of the musculoskeletal system ,RC925-935 - Abstract
Background: Circulating regulatory T cells (Tregs) are responsible for mediating immune tolerance and maintaining immunological homeostasis. Decreases in Tregs may be involved in the onset of rheumatoid arthritis (RA). Low-dose interleukin-2 (IL-2) has been considered for the treatment of inflammatory diseases mediated by T cells. This study focused on the status of circulating CD4 + T subsets and the clinical feasibility of IL-2 therapies in patients with RA. Methods: The subjects included 888 patients with RA and 100 healthy controls (HCs); 233 RA patients received IL-2 treatment with 0.5 million international units (MIU)/day from days 1 through 5. The demographic features, disease activity, and levels of CD4+T cells measured by modified flow cytometry were collected in all RA patients before and after treatment. Results: RA patients had lower absolute Treg counts (but not Th17) compared with HCs, which was associated with disease activity; previously treated RA patients had the fewest circulating Tregs ( p
- Published
- 2021
- Full Text
- View/download PDF
25. Candidacy for Cochlear Implantation in Prelingual Profoundly Deaf Adult Patients
- Author
-
Ghizlene Lahlou, Hannah Daoudi, Evelyne Ferrary, Huan Jia, Marion De Bergh, Yann Nguyen, Olivier Sterkers, and Isabelle Mosnier
- Subjects
cochlear implant ,prelingual profound hearing loss ,speech perception ,quality of life ,Medicine - Abstract
Cochlear implantation is usually not recommended for prelingual profoundly deaf adults, although some of these patients might benefit from it. This study aims to define the candidates for cochlear implantation in this population. This retrospective study reviewed 34 prelingual profoundly deaf patients who had received a cochlear implant at 32 ± 1.7 years old (16–55), with at least 1 year of follow-up. Speech perception and quality of life were assessed before and 3, 6, and 12 months after cochlear implantation, then every year thereafter. According to the word speech intelligibility in quiet (WSI) 1 year after implantation, two groups were identified: good performer (GP) with WSI ≥ 50% (n = 15), and poor performer (PP) with WSI ≤ 40% (n = 19). At the 1 year mark, mean WSI improved by 28 ± 4.6% (−20–100) (p < 0.0001). In GP, the intelligibility for words and sentences, communication and quality of life scales improved. In PP, the communication scale improved, but not auditory performance or quality of life. GP and PP differed pre-operatively in speech production, communication abilities, and WSI in best-aided conditions. In prelingual profoundly deaf adults, a dramatic auditory performance benefit could be expected after cochlear implantation if the patients have some degree of speech intelligibility in aided conditions and have developed oral communication and speech production.
- Published
- 2022
- Full Text
- View/download PDF
26. Engineering thermostable affinity proteins for use in high-throughput immunoassay formats
- Author
-
Huan Jia, Nazirulmubin Abdul Moomen, Jeanette Leong, Patthara Kongsuphol, Zhi Feng Sherman Lim, Carmen Sze Min Pui, Yuxuan Tan, Ki-Joo Sung, and Hadley D. Sikes
- Abstract
Protein engineering approach enables effective immobilisation of thermostable affinity proteins onto polystyrene materials.
- Published
- 2023
27. Simultaneous Bilateral Cochlear Implantation in Very Young Children Improves Adaptability and Social Skills: A Prospective Cohort Study
- Author
-
Ying, Chen, Yun, Li, Huan, Jia, Wenxi, Gu, Zhaoyan, Wang, Zhihua, Zhang, Minbo, Xue, Jingjie, Li, Wentao, Shi, Linlin, Jiang, Lu, Yang, Olivier, Sterkers, and Hao, Wu
- Subjects
Speech and Hearing ,Otorhinolaryngology - Abstract
To investigate the value of using the Gesell Development Diagnosis Scale (GDDS) to predict developmental outcomes in very young children who undergo simultaneous bilateral cochlear implantation.In this prospective cohort study, a repeated-measures investigation was conducted in a tertiary referral hospital. A total of 62 children receiving simultaneous bilateral cochlear implantations were enrolled from April 2017 to August 2018. They were divided into 2 groups depending on the operative age: "Infants" group (6 to 12 months, N = 38) or "Children" group (12 to 36 months, N = 24). Data on the surgical outcomes, auditory development, speech production, and developmental indicators were collected until 2 years after the initial fitting. The primary outcome measure was the GDDS, a neuropsychological development examination. Secondary outcomes included the following: complication rate, aided pure-tone average, Infant-Toddler Meaningful Auditory Integration Scale, Categories of Auditory Performance-II, Meaningful Use of Speech Scale, Speech Intelligibility Rating, and the LittlEARS Auditory Questionnaire.The mean ages at implantation in infants and children groups were 9.2 ± 1.17 and 16.6 ± 3.60 months, respectively. Significant differences were found in the social skills (p = 0.001) and adaptability (p = 0.031) domains of GDDS. The younger the age of bilateral cochlear implants surgery, the higher developmental quotient of language, social skills, and adaptability the child could achieve after 2 years. The complication rates in the infants and children groups were 0% versus 2.1% (p = 0.57). There was no surgical complication in the infants group. In the children group, 1 case with enlarged vestibular aqueduct and Mondini malformation had a receiver-implant misplacement on the right side (2%, 1/48). In the two groups, auditory performance and speech production had improved similarly. In the infants group, social skills developmental quotient at baseline had a significant positive relationship with Meaningful Use of Speech Scale after 2 years.Simultaneous bilateral cochlear implantation in younger children improves adaptability and social skills. GDDS is a sensitive tool of evaluating short-term effect of bilateral cochlear implants in neuropsychological development and constitutes a reliable predictor of speech production for the very younger pediatric cochlear implant users.
- Published
- 2022
28. Paracrine and epigenetic control of CAF-induced metastasis: the role of HOTAIR stimulated by TGF-ß1 secretion
- Author
-
Yu Ren, Huan-huan Jia, Yi-qi Xu, Xuan Zhou, Xiao-hui Zhao, Yun-fei Wang, Xin Song, Zhi-yan Zhu, Ting Sun, Yan Dou, Wei-ping Tian, Xiu-lan Zhao, Chun-sheng Kang, and Mei Mei
- Subjects
Carcinoma associated fibroblasts ,TGF-β1 ,HOTAIR ,Epigenetic control ,Metastasis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background The communication between carcinoma associated fibroblasts (CAFs) and cancer cells facilitate tumor metastasis. In this study, we further underlying the epigenetic mechanisms of CAFs feed the cancer cells and the molecular mediators involved in these processes. Methods MCF-7 and MDA-MB-231 cells were treated with CAFs culture conditioned medium, respectively. Cytokine antibody array, enzyme-linked immunosorbent assay, western blotting and immunofluorescence were used to identify the key chemokines. Chromatin immunoprecipitation and luciferase reporter assay were performed to explore the transactivation of target LncRNA by CAFs. A series of in vitro assays was performed with RNAi-mediated knockdown to elucidate the function of LncRNA. An orthotopic mouse model of MDA-MB-231 was conducted to confirm the mechanism in vivo. Results Here we reported that TGF-β1 was top one highest level of cytokine secreted by CAFs as revealed by cytokine antibody array. Paracrine TGF-β1 was essential for CAFs induced EMT and metastasis in breast cancer cells, which is a crucial mediator of the interaction between stromal and cancer cells. CAF-CM significantly enhanced the HOTAIR expression to promote EMT, whereas treatment with small-molecule inhibitors of TGF-β1 attenuated the activation of HOTAIR. Most importantly, SMAD2/3/4 directly bound the promoter site of HOTAIR, located between nucleotides -386 and -398, -440 and -452, suggesting that HOTAIR was a directly transcriptional target of SMAD2/3/4. Additionally, CAFs mediated EMT by targeting CDK5 signaling through H3K27 tri-methylation. Depletion of HOTAIR inhibited CAFs-induced tumor growth and lung metastasis in MDA-MB-231 orthotopic animal model. Conclusions Our findings demonstrated that CAFs promoted the metastatic activity of breast cancer cells by activating the transcription of HOTAIR via TGF-β1 secretion, supporting the pursuit of the TGF-β1/HOTAIR axis as a target in breast cancer treatment.
- Published
- 2018
- Full Text
- View/download PDF
29. Effect of botanical drugs in improving symptoms of hypertensive nephropathy: Analysis of real-world data, retrospective cohort, network, and experimental assessment
- Author
-
Huan, Jia-Ming, primary, Ma, Xi-Ting, additional, Li, Si-Yi, additional, Hu, Dong-Qing, additional, Chen, Hao-Yu, additional, Wang, Yi-Min, additional, Su, Xiao-Yi, additional, Su, Wen-Ge, additional, and Wang, Yi-Fei, additional
- Published
- 2023
- Full Text
- View/download PDF
30. Analysis of the Real-World Application Laws for Zhengan Xifeng Decoction in the Treatment of Hypertension Based on Machine Learning
- Author
-
Huan, Jia-Ming, primary, Cai, Dan-Yang, additional, Li, Jie, additional, Hua, Zhen, additional, Chen, Xiao-Qing, additional, Yang, Wen-Qing, additional, Hu, Yuan-Long, additional, Wang, Yi-Fei, additional, and Li, Yun-Lun, additional
- Published
- 2023
- Full Text
- View/download PDF
31. Association between low blood selenium concentrations and poor hand grip strength in United States adults participating in NHANES (2011–2014)
- Author
-
Jingjing Pei, Lailai Yan, Yuwan Wu, Xi Zhang, Huan Jia, and Huajun Li
- Subjects
Nutrition and Dietetics ,Physiology ,Physiology (medical) ,Endocrinology, Diabetes and Metabolism ,General Medicine - Abstract
The trace element selenium, which is found in selenoproteins, plays an antioxidant role in preventing muscle tissue injury. A positive association between selenium concentrations and hand grip strength has been reported in older adults; however, the evidence of this association is scarce in general adults. In this study, we aimed to evaluate the association between blood selenium concentrations and low hand grip strength using the data from the National Health and Nutrition Examination Survey 2011–2012 and 2013–2014 in the United States (US). Logistic regression was used to calculate the odds ratio (OR) of low hand grip strength, with blood selenium level adjusted for potential confounders. Among 8158 adults (women: 51.59%) with a mean age of 47 (range: 18–80) years, women and non-Hispanic Blacks tended to have low blood selenium concentrations. Notably, participants with high blood selenium concentrations (range, 178.1–192.5 µg/L) were more likely to have a low risk of low hand grip strength after adjusting for the potential covariates (OR: 0.60, 95% confidence interval (CI): 0.38–0.95) than those with low blood selenium concentrations. After excluding participants with chronic diseases, high blood selenium concentrations were found to be associated with a low risk of low hand grip strength (OR: 0.30, 95% CI: 0.14–0.65). A J-shaped relationship was found between selenium concentrations and low hand grip strength ( P for nonlinear trend
- Published
- 2023
32. Data from SET Domain–Containing Protein 4 Epigenetically Controls Breast Cancer Stem Cell Quiescence
- Author
-
Wei-Jun Yang, Yi-Ding Chen, Jiao-Jiao Zhou, Yue-Hong Wang, Guo-Ping Ding, Sheng-Nan Jia, Jin-Shu Yang, Xue-Ting Huang, Qian-Yun Lu, Yao-Shun Yang, Sun-Li Cai, Qian Zhu, Jing-Yi Feng, Xiao-Li Liu, Wen-Huan Jia, Yan-Fu Ding, and Sen Ye
- Abstract
Quiescent cancer stem cells (CSC) play important roles in tumorigenesis, relapse, and resistance to chemoradiotherapy. However, the determinants of CSC quiescence and how they sustain themselves to generate tumors and relapse beyond resistance to chemoradiotherapy remains unclear. Here, we found that SET domain–containing protein 4 (SETD4) epigenetically controls breast CSC (BCSC) quiescence by facilitating heterochromatin formation via H4K20me3 catalysis. H4K20me3 localized to the promoter regions and regulated the expression of a set of genes in quiescent BCSCs (qBCSC). SETD4-defined qBCSCs were resistant to chemoradiotherapy and promoted tumor relapse in a mouse model. Upon activation, a SETD4-defined qBCSC sustained itself in a quiescent state by asymmetric division and concurrently produced an active daughter cell that proliferated to produce a cancer cell population. Single-cell sequence analysis indicated that SETD4+ qBCSCs clustered together as a distinct cell type within the heterogeneous BCSC population. SETD4-defined quiescent CSCs were present in multiple cancer types including gastric, cervical, ovarian, liver, and lung cancers and were resistant to chemotherapy. SETD4-defined qBCSCs had a high tumorigenesis potential and correlated with malignancy and chemotherapy resistance in clinical breast cancer patients. Taken together, the results from our previous study and current study on six cancer types reveal an evolutionarily conserved mechanism of cellular quiescence epigenetically controlled by SETD4. Our findings provide insights into the mechanism of tumorigenesis and relapse promoted by SETD4-defined quiescent CSCs and have broad implications for clinical therapies.Significance:These findings advance our knowledge on the epigenetic determinants of quiescence in cancer stem cell populations and pave the way for future pharmacologic developments aimed at targeting drug-resistant quiescent stem cells.
- Published
- 2023
33. Supplementary Data from SET Domain–Containing Protein 4 Epigenetically Controls Breast Cancer Stem Cell Quiescence
- Author
-
Wei-Jun Yang, Yi-Ding Chen, Jiao-Jiao Zhou, Yue-Hong Wang, Guo-Ping Ding, Sheng-Nan Jia, Jin-Shu Yang, Xue-Ting Huang, Qian-Yun Lu, Yao-Shun Yang, Sun-Li Cai, Qian Zhu, Jing-Yi Feng, Xiao-Li Liu, Wen-Huan Jia, Yan-Fu Ding, and Sen Ye
- Abstract
Supplementary Table S1 and Supplementary Figure S1 to S11
- Published
- 2023
34. The mechanical design and fabrication of 162.5 MHz buncher for China accelerator driven sub-critical system injector II
- Author
-
Haihua Niu, Youtang Li, Yuan He, Bin Zhang, Shichun Huang, Chenzhang Yuan, Huan Jia, and Shenghu Zhang
- Subjects
Buncher ,Mechanical design ,Fabrication ,MEBT ,Coupler ,Tuner ,Nuclear engineering. Atomic power ,TK9001-9401 - Abstract
A buncher is one of the main pieces of equipment in the medium energy beam transport line (MEBT) for China accelerator driven sub-critical system (C-ADS) Injector II. To focus the beam longitudinally and match the beam for the acceptance of the superconducting linac section, two room temperature quarter wave resonator (QWR) bunchers with frequency of 162.5 MHz have been designed as parts of the MEBT. According to the beam transmission matching of the MEBT and the geometric parameters requirements of bunchers, the unique mechanical structure and the main processing technology of buncher cavities and their couplers and tuners are described in this paper. The fabrication of bunchers and their parts have been completed and tested at high power, the test results agree well with the design requirements. These bunchers work well for about two years in Institute of Modern Physics, Chinese Academy of Sciences.
- Published
- 2017
- Full Text
- View/download PDF
35. Novel Molecular-Level Insight into the Self-Healing Behavior and Mechanism of Polyurethane-Urea Elastomer Based on a Noncovalent Strategy
- Author
-
Jialiang Chen, Chichao Li, Huan Jia, Zhihua Shen, Rong Zhao, Tao Su, Bo Xiang, Xiujuan Wang, Danil W. Boukhvalov, Zhenyang Luo, and Yanlong Luo
- Subjects
Inorganic Chemistry ,Polymers and Plastics ,Organic Chemistry ,Materials Chemistry - Published
- 2022
36. A Decreased Absolute Number of Treg Cells in Patients with Active Rheumatoid Arthritis is Associated with Elevated Serum Osteopontin Levels with Disease Progression
- Author
-
Jian-Fang Xie, Jia Wang, Huan-Huan Bai, Jiao-Jiao He, Rui-Huan Jia, Xia Wang, Wen-Qi Zhang, Xiang-Cong Zhao, Xian-Cheng Zhang, Guang-Ying Liu, and Xiao-Feng Li
- Subjects
Pharmacology (medical) ,General Medicine - Published
- 2022
37. Thermal-Mechanical Analysis of Beam Window and Beam Tube for ADS Granular Flow Target
- Author
-
Weiping Deng, Yanbin Zhang, Huan Jia, Tao Wan, Weifeng Yang, Chengwen Qiang, Long Li, Fei Wang, Honglin Ge, Fei Ma, and Xueying Zhang
- Subjects
Nuclear Energy and Engineering - Published
- 2022
38. Summary and consensus in 7th International Conference on acoustic neuroma: An update for the management of sporadic acoustic neuromas
- Author
-
Hao Wu, Liwei Zhang, Dongyi Han, Ying Mao, Jun Yang, Zhaoyan Wang, Wang Jia, Ping Zhong, and Huan Jia
- Subjects
Otorhinolaryngology ,RF1-547 ,Surgery ,RD1-811 - Abstract
Sporadic vestibular schwannoma (acoustic neuroma) is a benign tumor arising from cochleovestibular nerve. Nowadays, various specialties and medical centers are treating this disease, and the multidisciplinary collaboration is the trend. In an effort to promote a uniform standard for reporting clinical results, even for treatment indications, the mainly controversies were posed and discussed during the 7th International Conference on acoustic neuroma, and the agreement was summarized by the Committee of this conference. The main symptoms grading and tumor stage should note its name of classification for making them comparable. The goal of the modern managements for vestibular schwannoma is to improve the quality of life with lower mortality, lower morbidity and better neurological function preservation. The experience of surgical team and their preference might be a major factor for the outcome. Because of lacking of long-term follow-up large data after radiotherapy, and with the development of microsurgery, radiotherapy is now less recommended except for recurrent cases or elderly patients. Keywords: Sporadic acoustic neuroma, Vestibular schwannoma, Management, Symptoms grading, Tumor stage, Microsurgery, Radiotherapy
- Published
- 2016
- Full Text
- View/download PDF
39. Development and translation of a paper-based top readout vertical flow assay for SARS-CoV-2 surveillance
- Author
-
Huan Jia, Eric A. Miller, Chia Ching Chan, Say Yong Ng, Mookkan Prabakaran, Meng Tao, Ian Shen-Yi Cheong, Sing Mei Lim, Ming Wei Chen, Xiaohong Gao, Abirami R., Megan E. McBee, Peter R. Preiser, Hadley D. Sikes, and Patthara Kongsuphol
- Subjects
body regions ,SARS-CoV-2 ,Point-of-Care Systems ,Biomedical Engineering ,COVID-19 ,Humans ,Bioengineering ,General Chemistry ,Pandemics ,Sensitivity and Specificity ,Biochemistry - Abstract
Surveillance of SARS-CoV-2 infection is critical for controlling the current pandemic. Antigen rapid tests (ARTs) provide a means for surveillance. Available lateral flow assay format ARTs rely heavily on nitrocellulose paper, raising challenges in supply shortage. Vertical flow assay (VFA) with cellulose paper as test material attracts much attention as a complementary test approach. However, current reported VFAs are facing challenges in reading the test signal from the bottom face of the test cassette, complicating the test workflow and hindering translation into rapid test application. Here, we address this gap with an enhanced VFA against SARS-CoV-2 N protein that adapts a cellulose pull-down test format allowing (1) one-step sample application at the top of the test cassette and (2) readout of the test signal from the top. We also demonstrate the feasibility of translating the enhanced VFA into a point-of-care application that can help in SARS-CoV-2 surveillance.
- Published
- 2022
40. Origins of biallelic inactivation of NF2 in neurofibromatosis type 2
- Author
-
Lu Xue, Weiwei He, Yi Zhang, Zhigang Wang, Hongsai Chen, Zhe Chen, Weidong Zhu, Dongmei Liu, Huan Jia, Yi Jiang, Zhaoyan Wang, and Hao Wu
- Subjects
Neurofibromatosis 2 ,Neurofibromin 2 ,Cancer Research ,Phenotype ,Oncology ,Mosaicism ,Genes, Neurofibromatosis 2 ,Basic and Translational Investigations ,Mutation ,otorhinolaryngologic diseases ,High-Throughput Nucleotide Sequencing ,Humans ,Neurology (clinical) - Abstract
Background Elucidating the mechanism by which biallelic inactivation evolved could provide a mechanistic understanding for NF2 tumorigenesis and also a rationale for clinical management. Methods A cohort of 60 NF2 patients was recruited. Next-generation sequencing of tumor and paired control samples was used to explore how NF2 mutations evolve in determining the clinical phenotypes. Results In total, 60 blood samples (one from each patient) and 61 (from 35 patients) NF2-associated tumors were collected. Next-generation sequencing of the blood samples detected “first hit” NF2 mutation in 35/60 donors (58.3%), 82.9% of which (29/35) bear heterozygous germline mutations, and 17.1% (6/35) of which are mosaics with variable allelic frequency (VAF). While a number of NF2 patients were found without germline mutation, most (57/61, 93.4%) NF2-associated tumors were identified with NF2 somatic mutation. We calculated the correlation between the onset latency of mosaic and germline NF2 allele carriers with the mosaicism VAF. The mosaicism VAF is negatively and linearly correlated to clinical symptom onset latency (R2 = 0.3677, P = .00351), suggesting biallelic inactivation probability is a linear function of “first hit” prevalence in the body. The second NF2 somatic mutation occurrence time positively correlates with the onset of clinical symptoms (R2 = 0.4151, P = .02633), suggesting tumor growth is linearly proportional to the time after biallelic inactivation. Conclusions Our results suggested that biallelic inactivation of NF2 evolved through neutral drift and preexisting first hit NF2 allele determines certain aspects of the clinical symptoms. Genetic diagnosis should be included in the diagnostic criteria and treatment consideration of NF2.
- Published
- 2021
41. Transfer line including vacuum differential system for a high-power windowless target
- Author
-
Yuanshuai Qin, Peng Zhang, Hanjie Cai, Yaling Zhang, Huan Jia, Chenzhang Yuan, Yuan He, Junhui Zhang, and Wenlong Zhan
- Subjects
Nuclear and particle physics. Atomic energy. Radioactivity ,QC770-798 - Abstract
As the highest power proton linear accelerator in continuous wave mode, China initiative Accelerator Driven sub-critical System linear accelerator will deliver 2.5 MW proton beam to the target inside the reactor. Vacuum transition from 5×10^{4} Pa to several 10^{-6} Pa is required at the accelerator to target section. A vacuum differential system located at the transfer line is designed to meet the requirement of the windowless target and upstream collimators are considered for power loss control in this paper. A vacuum test based on vacuum transition verifies that about 10 orders of magnitude vacuum transition could be achieved as required, and gas scattering simulation shows that the power loss and beam size increase are at an acceptable level before the last magnet.
- Published
- 2020
- Full Text
- View/download PDF
42. Strategies of eliminating nuclear waste using accelerator-driven system in the transition stage for sustainable and clean nuclear energy in China
- Author
-
Xunchao Zhang, Neng Pu, Hanjie Cai, Huan Jia, and Yuan He
- Subjects
Nuclear Energy and Engineering - Published
- 2023
43. A Rapid Temporal Bone Localization Method Based on Machine Visual Detection Markers
- Author
-
Jian-jun, Li, primary, Jian-ye, Zhuo, additional, Yue, Wu, additional, Zuo, Wang, additional, Zhen, Yao, additional, Huan, Jia, additional, Zhao-yan, Wang, additional, Ying, Chen, additional, Dao-min, Zhou, additional, and Ming-zhou, Yu, additional
- Published
- 2022
- Full Text
- View/download PDF
44. Predicting the Radiated Noise of a Submarine Propeller with Different Types of Control Surfaces
- Author
-
Fang-Nan Chang, Yu-Han Tsai, Shang-Sheng Chin, Jui-Hsiang Kao, Huan-Jia Xu, and Hua-Tung Wu
- Subjects
Physics ,Numerical Analysis ,Noise ,Applied Mathematics ,Mechanical Engineering ,Acoustics ,Propeller ,Submarine ,Ocean Engineering ,Flight control surfaces ,Civil and Structural Engineering - Abstract
_ The objective of this paper is to predict the noise radiated from submarine propellers with different control surface types (the cross- and X-type). When the propellers are free from cavitation, such as those of submarines at a diving depth, the radiated noise dominate, due to unsteady propeller forces. A well-known submarine model (DARPA SUBOFF) is taken as the computing sample. Simulations for hydrodynamics, including stern wakes and unsteady propeller forces, are carried out by using CFD (Computational Fluid Dynamics) technology, and the results are compared with the experimental data. The accuracy of the predicted noise depends on the CFD results. Comparisons between the CFD results and the experimental data are in good agreement. The CFD results are treated as dipole strengths in the linear wave theory to predict the radiated noise caused by the unsteady forces of the propeller. It is found that, when the control surface is of the X-type, the propeller inflow is more uniform, and the radiated noise can be decreased by about 5 dB compared to the cruciform control surface. Introduction When submarines are at diving depth, the noise generated by unsteady propeller forces (i.e., dipole strengths) will dominate. Because the juncture vortex caused by the sail makes the propeller inflow more nonuniform, the dipole strength will be enhanced and the radiated noise will be more noticeable. The uniformity of the wake field at the stern should be controlled well in order to restrain the radiated noise.
- Published
- 2021
45. Accelerating the optimization of vertical flow assay performance guided by a rational systematic model-based approach
- Author
-
Dousabel M.Y. Tay, Seunghyeon Kim, Yining Hao, Emma H. Yee, Huan Jia, Sydney M. Vleck, Makaya Chilekwa, Joel Voldman, and Hadley D. Sikes
- Subjects
History ,Polymers and Plastics ,Electrochemistry ,Biomedical Engineering ,Biophysics ,General Medicine ,Business and International Management ,Industrial and Manufacturing Engineering ,Biotechnology - Abstract
Rapid diagnostic tests (RDTs) have shown to be instrumental in healthcare and disease control. However, they have been plagued by many inefficiencies in the laborious empirical development and optimization process for the attainment of clinically relevant sensitivity. While various studies have sought to model paper-based RDTs, most have relied on continuum-based models that are not necessarily applicable to all operation regimes, and have solely focused on predicting the specific interactions between the antigen and binders. It is also unclear how the model predictions may be utilized for optimizing assay performance. Here, we propose a streamlined and simplified model-based framework, only relying on calibration with a minimal experimental dataset, for the acceleration of assay optimization. We show that our models are capable of recapitulating experimental data across different formats and antigen-binder-matrix combinations. By predicting signals due to both specific and background interactions, our facile approach enables the estimation of several pertinent assay performance metrics such as limit-of-detection, sensitivity, signal-to-noise ratio and difference. We believe that our proposed workflow would be a valuable addition to the toolset of any assay developer, regardless of the amount of resources they have in their arsenal, and aid assay optimization at any stage in their assay development process.
- Published
- 2022
46. Assessment of clinical sepsis-associated biomarkers in a septic mouse model
- Author
-
Jin-Ling Li, Ge Li, Xi-Zhong Jing, Yun-Feng Li, Qiu-Ying Ye, Huan-Huan Jia, Shu-Hua Liu, Xue-Jiao Li, Hang Li, Ren Huang, Yu Zhang, and Hui Wang
- Subjects
Medicine (General) ,R5-920 - Abstract
Objective Clinical sepsis-associated biomarkers were utilized in a cecal ligation and puncture (CLP) septic mouse model to provide a reference for investigating pathophysiological mechanisms and evaluating novel therapeutic interventions for sepsis. Methods Sepsis in mice was induced by CLP, and clinical biomarkers were evaluated (survival rate, blood physiological and biochemical indices, cytokines, hepatorenal function parameters, and blood coagulation). Results The mortality rate was >70%. The body temperature, blood pressure, and heart rate decreased within 48 h. Low lactic acid was found at 8 h. The CLP mice showed typical inflammatory symptoms with decreased white blood cells and procalcitonin and increased levels of soluble triggering receptor expressed on myeloid cells-1, interleukin (IL)-6, IL-10, tumor necrosis factor-α, macrophage inflammatory protein (MIP)-1α, MIP-1β, and MIP-2. The platelet count and activated partial thromboplastin time significantly decreased, and the prothrombin time and prothrombin time–international normalized ratio markedly increased. Phenotypes of multiple organ dysfunction were found in the CLP model, including increased liver alanine aminotransferase and aspartate transaminase; significantly reduced total protein, globulin, and serum albumin; increased blood urea nitrogen and creatinine; and decreased blood glucose. Conclusion The clinical features of the CLP mouse model were similar to those of human patients with sepsis.
- Published
- 2018
- Full Text
- View/download PDF
47. Hearing Protection Outcomes of Analog Electrode Arrays Coated with Different Drug-Eluting Polymer Films Implanted into Guinea Pig Cochleae
- Author
-
Xiayu Sun, Yuyu Huang, Fan Zhang, Min Liang, Jun Yang, Shule Hou, Haoran Yu, Huan Jia, and Junmin Chen
- Subjects
Polymers ,Guinea Pigs ,Pharmaceutical Science ,Nicotinamide adenine dinucleotide ,Dexamethasone ,Guinea pig ,chemistry.chemical_compound ,Drug Discovery ,Evoked Potentials, Auditory, Brain Stem ,medicine ,otorhinolaryngologic diseases ,Animals ,Hearing Loss ,Spiral ganglion ,Original Research ,Pharmacology ,Drug Design, Development and Therapy ,Chemistry ,Cytarabine ,cochlear implant ,drug-eluting electrode ,hearing protection ,NAD ,Electrodes, Implanted ,Cochlear Implants ,medicine.anatomical_structure ,Auditory brainstem response ,Electrode ,Female ,NAD+ kinase ,sense organs ,Corrigendum ,Drug carrier ,guinea pig ,Biomedical engineering ,medicine.drug - Abstract
Yuyu Huang,1,2,* Haoran Yu,3,* Min Liang,1,2 Shule Hou,1,2 Junmin Chen,1,2 Fan Zhang,1,2 Xiayu Sun,1,2 Huan Jia,2,4,5 Jun Yang1,2,5 1Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, Peopleâs Republic of China; 2Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases (14DZ2260300), Shanghai, 200125, Peopleâs Republic of China; 3ENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, Peopleâs Republic of China; 4Department of Otorhinolaryngology Head and Neck Surgery, Shanghai Ninth Peopleâs Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, Peopleâs Republic of China; 5Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, 200031, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Jun YangENT Ward of Xinhua Hospital, Floor 18, Building 8, 1665 Kongjiang Road, Yangpu District, Shanghai, 200092, Peopleâs Republic of ChinaTel +86 13764981808Email yangjun@xinhuamed.com.cnHuan JiaENT Ward of Ninth Peopleâs Hospital, Floor 2, Building 1, 639 Zhizaoju Road, Huangpu District, Shanghai, 200011, Peopleâs Republic of ChinaTel +86 13601797481Email huan_jia_orl@163.comObjective: To investigate the hearing protection outcomes of different drug-eluting analog electrode arrays implanted into guinea pig cochleae.Methods: Sixty guinea pigs were randomly divided into a negative control group and five experimental groups implanted separately with blank (drug carrier), dexamethasone (DXM), aracytine (Ara-C), Ara-C+DXM, and nicotinamide adenine dinucleotide (NAD+) eluting analog electrode arrays. Micro CT was used to supervise the surgical procedure. Auditory brainstem response (ABR) thresholds of the guinea pigs were measured and analyzed.Results and Conclusions: Compared with the negative control, all other groups showed a significant increase in ABR threshold (p< 0.001) after surgery. Among them, there was no obvious difference between the blank (0 vs 90 days: 59.70± 10.57 vs 64.60± 9.47 dB SPL) and the NAD+ group (0 vs 90 days: 59.90± 9.87 vs 64.70± 8.65 dB SPL). On the other hand, the ABR thresholds in the DXM (0 days: 58.10± 10.73 dB SPL; 90 days: 51.70± 9.07 dB SPL) and the Ara-C group (0 days: 59.00± 10.05 dB SPL; 90 days: 51.60± 8.48 dB SPL) decreased significantly compared with the former two groups (p< 0.001). However, the Ara-C+DXM group showed no further benefit (p> 0.05). In addition, a significantly higher survival rate of spiral ganglion neurons in cochleae was observed in the Ara-C and/or DXM groups.Keywords: guinea pig, cochlear implant, drug-eluting electrode, hearing protection
- Published
- 2021
48. Four peptidoglycan recognition proteins are indispensable for antibacterial immunity in the cigarette beetle Lasioderma serricorne (Fabricius)
- Author
-
Yi Yan, Ming-Huan Jia, Zhi-Jun Le, Kang-Kang Xu, Can Li, and Wen-Jia Yang
- Subjects
Staphylococcus aureus ,General Medicine ,Peptidoglycan ,Biochemistry ,Adenosine Monophosphate ,Immunity, Innate ,Anti-Bacterial Agents ,Coleoptera ,Structural Biology ,Larva ,Receptors, Pattern Recognition ,Escherichia coli ,Animals ,Amino Acids ,Carrier Proteins ,Molecular Biology - Abstract
The peptidoglycan recognition protein (PGRP), an important pattern recognition receptor of insects, is significant for reducing innate immunity and effective pest control. We cloned four PGRP genes (LsPGRP-LB, LsPGRP-LB1, LsPGRP-LE, and LsPGRP-SC2) from the cigarette beetle, Lasioderma serricorne (Fabricius), which encoded proteins of 216, 197, 317, and 190 amino acids, respectively. Three LsPGRPs were predominantly expressed in the larval and pupal stages, whereas LsPGRP-LE displayed high expression in adults. All the four LsPGRPs genes were highly expressed in the midgut and integument. Pathogen inoculation revealed that the four LsPGRPs actively responded to Escherichia coli and its peptidoglycan. The transcription levels of LsPGRP-LE and LsPGRP-SC2 increased significantly after Staphylococcus aureus stimulation. RNA interference-mediated knockdown of the four LsPGRPs led to increased larval mortality when challenged by E. coli, and the expression of four antimicrobial peptide genes (LsCole, LsAtt2, LsDef1 and LsDef2) had a significant decrease. Higher mortality and lower AMP expression were also observed in L. serricorne under S. aureus infection after silencing LsPGRP-LE and LsPGRP-SC2. Our results suggest that the four LsPGRP genes play important and distinct regulatory roles in the antibacterial defense response of L. serricorne.
- Published
- 2022
49. Development and cold-test of an RFQ-DTL coupled cavity
- Author
-
Wei-Ping Dou, Chen-Xing Li, Zhi-Jun Wang, Zong-Heng Xue, Xiao-Feng Jin, Zhi-Hui Li, Xiao-Xiao Li, Chao Wang, Wang-Sheng Wang, Huan Jia, Wei-Long Chen, Zhou-Li Zhang, Yuan-Shuai Qin, Xin Qi, and Yuan He
- Subjects
Nuclear and High Energy Physics ,Astronomy and Astrophysics ,Atomic and Molecular Physics, and Optics - Abstract
With the continuous strengthening of application requirements, neutron sources are becoming more and more compact, integrated and mobile. There are challenges in two aspects. The first is to achieve high transmission efficiency of high beam current physically, the second is to achieve integration and miniaturization technically. The coupling of RFQ and DTL is a feasible way. A preliminary study on the key physical problems in the RFQ-DTL coupled cavity has been accomplished. For the beam dynamics, the compact matching of RFQ and DTL was studied for the coupled cavity. Taking the compact physical design of the RFQ-DTL for deuterium ion beam of 30 mA with the energy of 11 MeV without MEBT as an example, we studied the smooth transition of the phase advance per unit length, the ability of RFQ transition cell to rotate beam in the transverse phase space, the transverse and longitudinal acceptance optimization of DTL. The RFQ and DTL can be matched transversely and longitudinally after removing MEBT, and the transmission efficiency is 98.7%. For direct coupling of four-vane RFQ and CH-DTL, we studied the realization of the coupling, the factors affecting the field amplitude ratio, the tuning of the cavity, and the influence of the superimposed field on the beam transmission. The field amplitude ratio between RFQ and CH-DTL is from 16.5 to 36.2. Under the influence of the superimposed field, the difference in normalized RMS emittance is less than 1%. We have designed, constructed and cold-test the 750 MHz model cavity. The normalized electric field coefficients measured and simulated are basically in agreement.
- Published
- 2022
50. Blood glucose mediated the effects of cognitive function impairment related to aluminum exposure in Chinese aluminum smelting workers
- Author
-
Xu, Shi-meng, primary, Pan, Bao-long, additional, Gao, Dan, additional, Zhang, Yun-wei, additional, Huan, Jia-ping, additional, Han, Xiao, additional, Song, Jing, additional, Wang, Lin-ping, additional, Zhang, Hui-fang, additional, Niu, Qiao, additional, and Lu, Xiao-ting, additional
- Published
- 2022
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.