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198 results on '"Huan, Shuangyan"'

5. Advances in the construction and application of chemiluminescence probes

Author

FENG Yurong, XU Shuai, HUAN Shuangyan, YUAN Lin, and ZHANG Xiaobing

Subjects
Technology
Abstract

In the chemiluminescence process, when the luminescent system and the response substrate undergo a chemical reaction, the dye molecules in the system are excited by the chemical energy generated by the chemical reaction and generate light signals. Since the external excitation light source is not required, the interference of background fluorescence is avoided and the imaging contrast will be improved, making it have unique advantages in in vivo imaging.In addition, the chemiluminescence system can be used as an internal light source to excite the photosensitizer for tumor therapy. In this review, the current type of luminescence systems, including Luminol, peroxyoxalate, 1, 2-dioxetane chemiluminescence system, as well as their luminescence mechanism and structural characteristics are reviewed,and their application in imaging and therapy is summarized. Finally, the development direction of these systems is prospected, and it is revealed that the development of a long-wavelength, high-brightness chemiluminescence system with a new structure is an effective strategy to the improve bioimaging and therapeutic effects.

Published
2022

10. Endogenous Enzyme-Driven Amplified DNA Nanocage Probe for Selective and Sensitive Imaging of Mature MicroRNAs in Living Cancer Cells

Author

Gao, Yingying, Gong, Chaonan, Chen, Mei, Huan, Shuangyan, Zhang, Xiao-Bing, and Ke, Guoliang

Abstract

Selective and sensitive imaging of intracellular mature microRNAs (miRNAs) is of great importance for biological process study and medical diagnostics. However, this goal remains challenging because of the interference of precursor miRNAs (pre-miRNAs) and the low abundance of mature miRNAs. Herein, we develop an endogenous enzyme-driven amplified DNA nanocage probe (Acage) for the selective and sensitive imaging of mature miRNAs in living cells. The Acage consists of a microRNA-responsive probe, an endogenous enzyme-driven fuel strand, and a DNA nanocage framework with an inner cavity. Benefiting from the size selectivity of DNA nanocage, smaller mature miRNAs rather than larger pre-miRNAs are allowed to enter the cavity of DNA nanocage for molecular recognition; thus, Acage can significantly reduce the signal interference of pre-miRNAs. Moreover, with the driving force of an endogenous enzyme apurinic/apyrimidinic endonuclease 1 (APE1) for efficient signal amplification, Acage enables sensitive intracellular miRNA imaging without an additional external intervention. With these features, Acage was successfully applied for intracellular imaging of mature miRNAs during drug treatment. We believe that this strategy provides a promising pathway for better understanding the functions of mature microRNAs in biological processes and medical diagnostics.

Published
2024
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20. A controllable self-localized imaging strategy capable of synchronous in situ tracking of local changes in intracellular bioactive small-molecules.

Author

Li, Ke, Xu, Shuai, Wang, Benhua, Huan, Shuangyan, Song, Xiangzhi, Yuan, Lin, and Zhang, Xiao-Bing

Abstract

In situ tracking and localization of ubiquitous bioactive small molecules (BSMs) within their native habitats is particularly challenging because of their low-molecular weight and widespread distribution properties. We report the proof of concept of a synchronous in situ imaging strategy, whereby the representative BSM amino-biothiols (ABs) mediate activation of the self-localizable probe HYPQS, thereby releasing insoluble emissive precipitates to afford holistic distribution information of ABs. Notably, three organelle-targetable ABs inhibitors were innovatively fabricated for directed clearance of ABs in particular organelles, providing a powerful aid for HYPQS to achieve programmed in situ tracking of ABs in different organelles "on demand". Biological transmission electron microscopy images confirmed that this probe released insoluble emissive precipitates at the reaction sites, which is of primary importance for achieving synchronous in situ tracking of BSMs. Furthermore, the probe HYPQS was successfully applied to monitor the dynamic changes in the endogenous ABs pool during diverse cell events. This strategy opens a promising avenue for investigating the undiscovered functional mechanism of local BSMs in relevant biological processes. [ABSTRACT FROM AUTHOR]

Published
2023
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23. A controllable self-localized imaging strategy capable of synchronous in situtracking of local changes in intracellular bioactive small-molecules

Author

Li, Ke, Xu, Shuai, Wang, Benhua, Huan, Shuangyan, Song, Xiangzhi, Yuan, Lin, and Zhang, Xiao-Bing

Abstract

In situtracking and localization of ubiquitous bioactive small molecules (BSMs) within their native habitats is particularly challenging because of their low-molecular weight and widespread distribution properties. We report the proof of concept of a synchronous in situimaging strategy, whereby the representative BSM amino-biothiols (ABs) mediate activation of the self-localizable probe HYPQS, thereby releasing insoluble emissive precipitates to afford holistic distribution information of ABs. Notably, three organelle-targetable ABs inhibitors were innovatively fabricated for directed clearance of ABs in particular organelles, providing a powerful aid for HYPQS to achieve programmed in situtracking of ABs in different organelles “on demand”. Biological transmission electron microscopy images confirmed that this probe released insoluble emissive precipitates at the reaction sites, which is of primary importance for achieving synchronous in situtracking of BSMs. Furthermore, the probe HYPQS was successfully applied to monitor the dynamic changes in the endogenous ABs pool during diverse cell events. This strategy opens a promising avenue for investigating the undiscovered functional mechanism of local BSMs in relevant biological processes.

Published
2023
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28. Suggestion for Teaching Contents and Teaching Requirements of Analytical Chemistry Course for Chemistry Majors

Author

Wang, Yuzhi, primary, Yang, Yi, additional, Wei, Qin, additional, Li, Gongke, additional, Zhang, Wenqing, additional, Cao, Qiue, additional, Zheng, Chengbin, additional, Xue, Bingchun, additional, Yang, Xiaohai, additional, Wu, Zhaoyang, additional, Chen, Zengping, additional, Song, Guosheng, additional, and Huan, Shuangyan, additional

Published
2022
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48. Precipitated Fluorophore-Based Molecular Probe for In SituImaging of Aminopeptidase N in Living Cells and Tumors

Author

Liu, Yongchao, Xu, Chengyan, Liu, Hong-Wen, Teng, Lili, Huan, Shuangyan, Yuan, Lin, and Zhang, Xiao-Bing

Abstract

Aminopeptidase N (APN) is capable of cleaving N-terminal amino acids from peptides with alanine in the N-terminal position and plays a key role in the growth, migration, and metastasis of cancer. However, reliable in situinformation is hard to be obtained with the current APN-responsive molecular probes because the released fluorophores are cytoplasmic soluble and thus rapidly depart from the enzymatic reaction sites and spread out all over the cytoplasm. Here, we report a de novoprecipitated fluorophore, HBPQ, which is completely insoluble in water and shows strong yellow solid emission when excited with a 405 nm laser. Owing to the controllable solid fluorescence of HBPQby the protection–deprotection of phenolic hydroxyl, we further utilized HBPQto design an APN-responsive fluorogenic probe (HBPQ-A) for the imaging of intracellular APN. Importantly, HBPQ-Acan not only perform in situimaging of APN in different organelles (e.g., lysosomes, mitochondria, endoplasmic reticula, and so forth) but also display a stable and indiffusible fluorescent signal for reliable mapping of the distribution of APN in living cells. In addition, through real-time imaging of APN in 4T1 tumors, we found that the fluorescent signal with high fidelity generated by HBPQ-Acould remain constant even after 12 h, which further confirmed its diffusion-resistant ability and long-term reliable imaging ability. We believe that the precipitated fluorophore may have great potential for long-term in situimaging.

Published
2021
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50. Oxygen-Embedded Pentacene Based Near-Infrared Chemiluminescent Nanoprobe for Highly Selective and Sensitive Visualization of Peroxynitrite In Vivo

Author

Wang, Bingzhe, Wang, Youjuan, Wang, Yanpei, Zhao, Yan, Yang, Chan, Zeng, Zebing, Huan, Shuangyan, Song, Guosheng, and Zhang, Xiaobing

Abstract

Peroxynitrite (ONOO–) is involved in neurodegenerative, inflammatory, cardiovascular disorders, cancers, and other pathological progress. However, current imaging methods for sensing ONOO–usually suffer from high background/autofluorescence for fluorescent probes and poor selectivity/short emission wavelength for chemiluminescent probes. Herein, we present a novel chemiluminescent molecule (oxygen-embedded quinoidal pentacene) responsive to ONOO–for the first time, on the basis of which we rationally construct a near-infrared nanoprobe for detecting ONOO–via chemiluminescence resonance energy transfer (CRET) mechanism. Notably, our nanoprobe exhibits good selectivity, ultrahigh sensitivity (nanomole level), low background noise, fast response, and high water solubility. Moreover, the near-infrared emission from CRET offers higher tissue penetration of the chemiluminescent signal. Finally, our nanoprobe is further successfully applied to detecting endogenous ONOO–in mice with abdominal inflammation, drug-induced hepatotoxicity, or tumor models in vivo. In summary, the self-luminescing nanoprobes can act as an alternative visualizable tool for illuminating the mechanism of ONOO–involved in the specific pathological process.

Published
2020
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