1. Regulation of Transcription Factor Yin Yang 1 by SET7/9-mediated Lysine Methylation
- Author
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Wen-juan Zhang, Hai-feng Shen, Weiwei Gao, Xiao-Nan Wu, Jiancheng Ding, Ming-feng Huang, Xing-yi Shu, Jia Yi, Tao-tao Shi, Huan-teng Xu, Bing-ling Peng, Rong-quan Xiao, Fei-fei Wang, and Wen Liu
- Subjects
0301 basic medicine ,Methyltransferase ,Transcription, Genetic ,Biology ,Transfection ,Methylation ,Article ,03 medical and health sciences ,Protein Domains ,Transcription (biology) ,Humans ,RNA, Small Interfering ,Transcription factor ,RNA-Directed DNA Methylation ,YY1 Transcription Factor ,Cell Proliferation ,Genetics ,Multidisciplinary ,030102 biochemistry & molecular biology ,Lysine ,EZH2 ,Histone-Lysine N-Methyltransferase ,Recombinant Proteins ,Cell biology ,HEK293 Cells ,030104 developmental biology ,Histone methyltransferase ,embryonic structures ,DNA methylation ,CRISPR-Cas Systems ,Protein Processing, Post-Translational ,HeLa Cells ,Plasmids - Abstract
Yin Yang 1 (YY1) is a multifunctional transcription factor shown to be critical in a variety of biological processes. Although it is regulated by multiple types of post-translational modifications (PTMs), whether YY1 is methylated, which enzyme methylates YY1 and hence the functional significance of YY1 methylation remains completely unknown. Here we reported the first methyltransferase, SET7/9 (KMT7), capable of methylating YY1 at two highly conserved lysine (K) residues, K173 and K411, located in two distinct domains, one in the central glycine-rich region and the other in the very carboxyl-terminus. Functional studies revealed that SET7/9-mediated YY1 methylation regulated YY1 DNA-binding activity both in vitro and at specific genomic loci in cultured cells. Consistently, SET7/9-mediated YY1 methylation was shown to involve in YY1-regulated gene transcription and cell proliferation. Our findings revealed a novel regulatory strategy, methylation by lysine methyltransferase, imposed on YY1 protein and linked YY1 methylation with its biological functions.
- Published
- 2016