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It was of great significance to clarify the laws and characteristics of nitrogen loss in tea plantation intercropping for scientifically evaluating the emission reduction effect of agricultural non-point source pollution in the ecological tea plantations. By taking the ecological tea plantation in Huanxi Town, Fuzhou City, Fujian Province as the research object, the long-term location experiment was carried out by using the runoff plot method. The volume of runoff from tea plantations under the conditions of monocropping along the downward slope (T1), intercropping along the downward slope (T2), monocropping in the terrace (T3) and intercropping in the terrace (T4) in 16 rainfall events from 2016 to 2018 was observed, in order to analyze the variation of nitrogen concentration and its loss amout in runoff. The intercropping plant was Paspalum notatum. The results showed that: according to the runoff volume in the downward slope (T1, T2) and terrace (T3, T4), the volume of runoff from tea plantations in intercropping was lower than that in monocropping. Along the downward slope, the TN concentration, AN concentration, NN concentration, TN loss amount, AN loss amount and NN loss amount in intercropping decreased by 32.0%, 44.4%, 23.8%, 38.5%, 49.8% and 30.8% compared with that in monocropping. In the terrace, the TN concentration, AN concentration, NN concentration, TN loss amount, AN loss amount and NN loss amount in intercropping decreased by 26.4%, 37.9%, 21.9%, 49.5%, 51.7% and 53.3% compared with that in monocropping. There was a significant correlation between the rainfall and runoff volume. [ABSTRACT FROM AUTHOR]

This work was to report preparation and quality control of targeted microbubbles (MBt) via biotin-avidin bridge, specifically verification of the 'biotin-avidin-biotin' sandwich structure. (1) Lipid microbubbles filled with sulphur hexafluoride were produced by sonication and compared with commercially available microbubbles. (2) MBt were produced via biotin-avidin bridge. Specifically, the 'biotin-avidin-biotin' sandwich structure on the surface of MBt was verified in vitro using fluorescence. (3) Adhesion of alpha(v)beta(3)-integrin targeted MBt (MBααvββ3) to human umbilical vein endothelial cells (HUVECs) was tested using the parallel plate flow chamber (PPFC). 'Biotin-avidin' is a reliable method to attach molecular probe onto the surface of microbubbles. [ABSTRACT FROM AUTHOR]