25 results on '"Huang, Lu-Sheng"'
Search Results
2. Impact of hemoglobin variability on cardiovascular mortality in maintenance hemodialysis patients
- Author
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Lin, Fu-Jun, Zhang, Xi, Huang, Lu-Sheng, Ji, Gang, Huang, Hai-Dong, Xie, Yun, Jiang, Geng-Ru, Zhou, Xin, and Lu, Wei
- Published
- 2018
- Full Text
- View/download PDF
3. Effects of carcass weight, sex and breed composition on meat cuts and carcass trait in finishing pigs
- Author
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XIE, Lei, primary, QIN, Jiang-tao, additional, RAO, Lin, additional, CUI, Deng-shuai, additional, TANG, Xi, additional, XIAO, Shi-jun, additional, ZHANG, Zhi-yan, additional, and HUANG, Lu-sheng, additional
- Published
- 2022
- Full Text
- View/download PDF
4. Effects of carcass weight, sex and breed composition on meat cuts and carcass trait in finishing pigs
- Author
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XIE, Lei, QIN, Jiang-tao, RAO, Lin, CUI, Deng-shuai, TANG, Xi, XIAO, Shi-jun, ZHANG, Zhi-yan, and HUANG, Lu-sheng
- Abstract
Pork cutting is a very important processing in promoting economic appreciation across the swine business chain. The goal of this research is to determine the proportion and weight of meat cuts, as well as to analyze the effects of carcass weight, sex and breed composition on meat cuts. Simultaneously, we investigate the correlation between meat cuts, carcass traits and meat quality traits. To assess 17 meat cut traits, 12 carcass traits and 6 meat quality traits, we sample 2 012 pigs from four breeds, including Landrace (LD), Yorkshire (YK), Landrace Yorkshire (LY), and Duroc Landrace Yorkshire (DLY). The results showed that carcass weight, sex and breed composition have significant effects on the weight and proportion of most meat cuts. The proportion of cuts for muscle and bone decrease as carcass weight grows, whereas the proportion of cuts for fat increases. Moreover, the thickness of four-point backfat was significantly increasing (P<0.001) with increase of carcass weights, indicating that large amount of intaking energy in the late finishing stage was used for fat deposition. Besides, the proportion of Shoulder cut (SC) and Back fat (BF) in barrows was significantly higher (P<0.001) than that in sows, whereas the Leg cut (LC) showed the opposite trend. The Loin (LO) proportion and Loin muscle area (LMA) of barrows were significantly lower (P<0.001), but the proportion of fat areas in the image (PFAI) and visual marbling score (VMS) were significantly higher (P<0.001) than those of sows, respectively. In terms of breeds, LD had the longest straight carcass length, significantly longer (P<0.001) than the other three breeds, which partially explains why LD had the largest proportion of the Middle cut (MC). Moreover, the proportion of SC in DLY was the highest. Last but not least, the correlations between the proportions of most meat cuts, and also between meat cuts and meat quality or carcass traits were low or not significant (P>0.05). The effects of carcass weight, sex and breed composition on the meat cuts, meat quality and carcass traits are breed and growth stage dependent. It also reflects the asynchrony of the growth curve between different sexes. Our results laid an important foundation for breeding pig carcass cuts and composition.
- Published
- 2023
- Full Text
- View/download PDF
5. Genetic variations of the porcine PRKAG3 gene in Chinese indigenous pig breeds
- Author
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Zhou Li-Hua, Li Lin, Ai Hua-Shui, Guo Yuan-Mei, Ding Neng-Shui, Ren Jun, Ma Jun-Wu, Huang Lu-Sheng, and Chen Cong-Ying
- Subjects
pig ,PRKAG3 gene ,meat quality ,Animal culture ,SF1-1100 ,Genetics ,QH426-470 - Abstract
Abstract Four missense substitutions (T30N, G52S, V199I and R200Q) in the porcine PRKAG3 gene were considered as the likely candidate loci affecting meat quality. In this study, the R200Q substitution was investigated in a sample of 62 individuals from Hampshire, Chinese Min and Erhualian pigs, and the genetic variations of T30N, G52S and V199I substitutions were detected in 1505 individuals from 21 Chinese indigenous breeds, 5 Western commercial pig breeds, and the wild pig. Allele 200R was fixed in Chinese Min and Erhualian pigs. Haplotypes II-QQ and IV-QQ were not observed in the Hampshire population, supporting the hypothesis that allele 200Q is tightly linked with allele 199V. Significant differences in allele frequencies of the three substitutions (T30N, G52S and V199I) between Chinese indigenous pigs and Western commercial pigs were observed. Obvious high frequencies of the "favorable" alleles 30T and 52G in terms of meat quality were detected in Chinese indigenous pigs, which are well known for high meat quality. However, the frequency of the "favorable" allele 199I, which was reported to have a greater effect on meat quality in comparison with 30T and 52G, was very low in all of the Chinese indigenous pigs except for the Min pig. The reasons accounting for this discrepancy remain to be addressed. The presence of the three substitutions in purebred Chinese Tibetan pigs indicates that the three substitutions were ancestral mutations. A novel A/G substitution at position 51 in exon 1 was identified. The results suggest that further studies are required to investigate the associations of these substitutions in the PRKAG3 gene with meat quality of Chinese indigenous pigs, and to uncover other polymorphisms in the PRKAG3 gene with potential effects on meat quality in Chinese indigenous pigs.
- Published
- 2004
- Full Text
- View/download PDF
6. De novo Cardiac Valve Calcification after Hemodialysis in End-Stage Renal Disease Patients Predicts Future Cardiovascular Events: A Longitudinal Cohort Study
- Author
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Lin, Fu-Jun, primary, Zhang, Xi, additional, Huang, Lu-Sheng, additional, Zhou, Xin, additional, Ji, Gang, additional, Luo, Rong, additional, Jiang, Geng-Ru, additional, and Lu, Wei, additional
- Published
- 2019
- Full Text
- View/download PDF
7. Genetic variations of the porcine PRKAG3 gene in Chinese indigenous pig breeds
- Author
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Huang, Lu-Sheng, Ma, Jun-Wu, Ren, Jun, Ding, Neng-Shui, Guo, Yuan-Mei, Ai, Hua-Shui, Li, Lin, Zhou, Li-Hua, and Chen, Cong-Ying
- Published
- 2004
- Full Text
- View/download PDF
8. AFLP markers for genomic DNA fingerprinting in pigs
- Author
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Ren Jun, Huang Lu-Sheng, Gary Evens, Ai Hua-Shui, Gao Jun, Chen Ke-Fei, and Ding Neng-Shui
- Subjects
Agronomy and Crop Science ,Biotechnology - Published
- 2004
9. Establishing and optimizing the system for sex determination of bovine preimplantation embryos
- Author
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Chen Cong-Ying, Huang Lu-Sheng, Chen Jing-Bo, Ding Neng-Shui, Zhou Li-Lua, and Xiao Hai-Xia
- Subjects
Agronomy and Crop Science ,Biotechnology - Published
- 2004
10. Genetic Variation of Porcine Prostaglandin-endoperoxide Synthase 2 (PTGS2) Gene and Its Association with Reproductive Traits in an Erhualian × Duroc F2 Population
- Author
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DING, Neng-Shui, primary, REN, Dong-Ren, additional, GUO, Yuan-Mei, additional, REN, Jun, additional, YAN, Ying, additional, MA, Jun-Wu, additional, CHEN, Ke-Fei, additional, and HUANG, Lu-Sheng, additional
- Published
- 2006
- Full Text
- View/download PDF
11. Genomic selection for meat quality traits based on VIS/NIR spectral information1
- Author
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TANG, Xi, XIE, Lei, YAN, Min, LI, Long-yun, YAO, Tian-xiong, LIU, Si-yi, XU, Wen-wu, XIAO, Shi-jun, DING, Neng-shui, ZHANG, Zhi-yan, and HUANG, Lu-sheng
- Abstract
The principle of genomic selection (GS) entails estimating breeding values (BVs) by summing all the SNP polygenic effects. The VIS/NIRS wavelength and abundance values can directly reflect the concentrations of chemical substances, and the measurement of meat traits by VIS/NIRS is similar to the processing of genomic selection data by summing all “polygenic effects” associated with spectral feature peaks. Therefore, it is meaningful to investigate the incorporation of VIS/NIRS information into GS models to establish an efficient and low-cost breeding model. In this study, we measured 6 meat quality traits in 359 Duroc×Landrace×Yorkshire pigs from Guangxi, China, and genotyped them with high-density SNP chips. According to the completeness of the information for the target population, we proposed four breeding strategies applied to different scenarios: I, only spectral and genotypic data exist for the target population; II, only spectral data exist for the target population; III, only spectral and genotypic data but with different prediction processes exist for the target population; and IV, only spectral and phenotypic data exist for the target population. The four scenarios were used to evaluate the GEBV accuracy by increasing the VIS/NIR spectral information. In the results of the 5-fold cross-validation, the genetic algorithm showed remarkable potential for preselection of feature wavelengths. The breeding efficiency of Strategies II, III, and IV was superior to that of traditional GS for most traits, and the GEBV prediction accuracy was improved by 32.2%, 40.8% and 15.5% respectively on average. Among them, the prediction accuracy of Strategy II for Fat (%) even improved by 50.7% compared to traditional GS. The GEBV prediction accuracy of Strategy I was nearly identical to that of traditional GS, and the fluctuation range was less than 7%. Moreover, the breeding cost of the four strategies was lower than that of traditional GS methods, with Strategy IV being the lowest as it ’did not require genotyping. Our findings demonstrate that GS methods based on VIS/NIRS data have significant predictive potential and are worthy of further research to provide a valuable reference for the development of effective and affordable breeding strategies.
- Published
- 2023
- Full Text
- View/download PDF
12. Genetic Variation of Porcine Prostaglandin-endoperoxide Synthase 2 (PTGS2) Gene and Its Association with Reproductive Traits in an Erhualian × Duroc F 2Population
- Author
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DING, Neng-Shui, REN, Dong-Ren, GUO, Yuan-Mei, REN, Jun, YAN, Ying, MA, Jun-Wu, CHEN, Ke-Fei, and HUANG, Lu-Sheng
- Abstract
Identification of major genes that genetically impact female fertility is important for successful selection of high prolificacy pig lines. Because it is the rate-limiting enzyme in the conversion of arachidonic acid to prostaglandins (PGs), which are important for ovulation, fertilization, implantation, decidualization and parturition, prostaglandin-endoperoxide synthase 2 gene ( PTGS2) is a potential candidate gene affecting porcine reproductive traits. In this study, a PCR-RFLP was used to genotype a total of 1 031 animals, including 661 from twelve Chinese local pig breeds, 190 from three Western pig breeds and 180 F 2sows from Nanchang pig resource family. Differences in frequency distributions of PTGS2 among twelve Chinese and three Western pig breeds and populations generally agree with their prolificacy. The allele frequencies in Lower Changjiang River Basin Type pig breeds, North China Type and Central China Type breeds are significantly different from those in South China Type, Plateau Type and Western pig breeds (P<0.001). And no significant differences were observed among Lower Changjiang River Basin Type, North China Type, Central China Type pig breeds, between South China Type and Western pig breeds, in part because of similar fertility patterns. And notable associations as well as reliable additive and dominant effects were not detected in an Erhualian x Duroc F 2population (P>0.05). Whereas, there is a trend for animals with one copy of the favourable A allele to have an increased TNB (total number of piglet born) and TBA (the number of piglets born alive) and a decreased SB (stillborn pigs) trait. Considering its crucial role in reproductive pathways, the PTGS2 gene deserves further study.
- Published
- 2006
- Full Text
- View/download PDF
13. Genetic variations of the porcine PRKAG3gene in Chinese indigenous pig breeds
- Author
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Huang, Lu-Sheng, Ma, Jun-Wu, Ren, Jun, Ding, Neng-Shui, Guo, Yuan-Mei, Ai, Hua-Shui, Li, Lin, Zhou, Li-Hua, and Chen, Cong-Ying
- Abstract
Four missense substitutions (T30N, G52S, V199Iand R200Q) in the porcine PRKAG3gene were considered as the likely candidate loci affecting meat quality. In this study, the R200Qsubstitution was investigated in a sample of 62 individuals from Hampshire, Chinese Min and Erhualian pigs, and the genetic variations of T30N, G52Sand V199Isubstitutions were detected in 1505 individuals from 21 Chinese indigenous breeds, 5 Western commercial pig breeds, and the wild pig. Allele 200Rwas fixed in Chinese Min and Erhualian pigs. Haplotypes II-QQand IV-QQwere not observed in the Hampshire population, supporting the hypothesis that allele 200Qis tightly linked with allele 199V. Significant differences in allele frequencies of the three substitutions (T30N, G52Sand V199I) between Chinese indigenous pigs and Western commercial pigs were observed. Obvious high frequencies of the "favorable" alleles 30Tand 52Gin terms of meat quality were detected in Chinese indigenous pigs, which are well known for high meat quality. However, the frequency of the "favorable" allele 199I, which was reported to have a greater effect on meat quality in comparison with 30Tand 52G, was very low in all of the Chinese indigenous pigs except for the Min pig. The reasons accounting for this discrepancy remain to be addressed. The presence of the three substitutions in purebred Chinese Tibetan pigs indicates that the three substitutions were ancestral mutations. A novel A/G substitution at position 51 in exon 1 was identified. The results suggest that further studies are required to investigate the associations of these substitutions in the PRKAG3gene with meat quality of Chinese indigenous pigs, and to uncover other polymorphisms in the PRKAG3gene with potential effects on meat quality in Chinese indigenous pigs.
- Published
- 2004
- Full Text
- View/download PDF
14. Pig H3K4me3, H3K27ac, and gene expression profiles reveal reproductive tissue-specific activity of transposable elements.
- Author
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Jiang T, Zhou ZM, Ling ZQ, Zhang Q, Wu ZZ, Yang JW, Yang SY, Yang B, and Huang LS
- Subjects
- Humans, Male, Mice, Animals, Swine genetics, Histones genetics, Histones metabolism, RNA, Messenger, Protein Serine-Threonine Kinases genetics, Protein Serine-Threonine Kinases metabolism, Homeodomain Proteins genetics, Homeodomain Proteins metabolism, Trans-Activators genetics, Trans-Activators metabolism, DNA Transposable Elements genetics, Transcriptome
- Abstract
Regulatory sequences and transposable elements (TEs) account for a large proportion of the genomic sequences of species; however, their roles in gene transcription, especially tissue-specific expression, remain largely unknown. Pigs serve as an excellent animal model for studying genomic sequence biology due to the extensive diversity among their wild and domesticated populations. Here, we conducted an integrated analysis using H3K27ac ChIP-seq, H3K4me3 ChIP-seq, and RNA-seq data from 10 different tissues of seven fetuses and eight closely related adult pigs. We aimed to annotate the regulatory elements and TEs to elucidate their associations with histone modifications and mRNA expression across different tissues and developmental stages. Based on correlation analysis between mRNA expression and H3K27ac and H3K4me3 peak activity, results indicated that H3K27ac exhibited stronger associations with gene expression than H3K4me3. Furthermore, 1.45% of TEs overlapped with either the H3K27ac or H3K4me3 peaks, with the majority displaying tissue-specific activity. Notably, a TE subfamily (LTR4C_SS), containing binding motifs for SIX1 and SIX4, showed specific enrichment in the H3K27ac peaks of the adult and fetal ovaries. RNA-seq analysis also revealed widespread expression of TEs in the exons or promoters of genes, including 4 688 TE-containing transcripts with distinct development stage-specific and tissue-specific expression. Of note, 1 967 TE-containing transcripts were enriched in the testes. We identified a long terminal repeat (LTR), MLT1F1, acting as a testis-specific alternative promoter in SRPK2 (a cell cycle-related protein kinase) in our pig dataset. This element was also conserved in humans and mice, suggesting either an ancient integration of TEs in genes specifically expressed in the testes or parallel evolutionary patterns. Collectively, our findings demonstrate that TEs are deeply embedded in the genome and exhibit important tissue-specific biological functions, particularly in the reproductive organs.
- Published
- 2024
- Full Text
- View/download PDF
15. Single-cell profiling of the pig cecum at various developmental stages.
- Author
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Xiao YY, Zhang Q, Huang F, Rao L, Yao TX, Yang SY, Xie L, Zou XX, Cai LP, Yang JW, Yang B, and Huang LS
- Subjects
- Humans, Mice, Animals, Swine, Gastrointestinal Tract, Gene Expression Profiling veterinary, Epithelial Cells, Cecum metabolism, Intestines
- Abstract
The gastrointestinal tract is essential for food digestion, nutrient absorption, waste elimination, and microbial defense. Single-cell transcriptome profiling of the intestinal tract has greatly enriched our understanding of cellular diversity, functional heterogeneity, and their importance in intestinal tract development and disease. Although such profiling has been extensively conducted in humans and mice, the single-cell gene expression landscape of the pig cecum remains unexplored. Here, single-cell RNA sequencing was performed on 45 572 cells obtained from seven cecal samples in pigs at four different developmental stages (days (D) 30, 42, 150, and 730). Analysis revealed 12 major cell types and 38 subtypes, as well as their distinctive genes, transcription factors, and regulons, many of which were conserved in humans. An increase in the relative proportions of CD8
+ T and Granzyme A (low expression) natural killer T cells (GZMAlow NKT) cells and a decrease in the relative proportions of epithelial stem cells, Tregs, RHEX+ T cells, and plasmacytoid dendritic cells (pDCs) were noted across the developmental stages. Moreover, the post-weaning period exhibited an up-regulation in mitochondrial genes, COX2 and ND2 , as well as genes involved in immune activation in multiple cell types. Cell-cell crosstalk analysis indicated that IBP6+ fibroblasts were the main signal senders at D30, whereas IBP6- fibroblasts assumed this role at the other stages. NKT cells established interactions with epithelial cells and IBP6+ fibroblasts in the D730 cecum through mediation of GZMA-F2RL1/F2RL2 pairs. This study provides valuable insights into cellular heterogeneity and function in the pig cecum at different development stages.- Published
- 2024
- Full Text
- View/download PDF
16. Scan of the endogenous retrovirus sequences across the swine genome and survey of their copy number variation and sequence diversity among various Chinese and Western pig breeds.
- Author
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Chen JQ, Zhang MP, Tong XK, Li JQ, Zhang Z, Huang F, Du HP, Zhou M, Ai HS, and Huang LS
- Subjects
- Animals, China, DNA Copy Number Variations, Humans, Swine genetics, Transplantation, Heterologous veterinary, Endogenous Retroviruses genetics
- Abstract
In pig-to-human xenotransplantation, the transmission risk of porcine endogenous retroviruses (PERVs) is of great concern. However, the distribution of PERVs in pig genomes, their genetic variation among Eurasian pigs, and their evolutionary history remain unclear. We scanned PERVs in the current pig reference genome (assembly Build 11.1), and identified 36 long complete or near-complete PERVs (lcPERVs) and 23 short incomplete PERVs (siPERVs). Besides three known PERVs (PERV-A, -B, and -C), four novel types (PERV-JX1, -JX2, -JX3, and -JX4) were detected in this study. According to evolutionary analyses, the newly discovered PERVs were more ancient, and PERV-Bs probably experienced a bottleneck ~0.5 million years ago (Ma). By analyzing 63 high-quality porcine whole-genome resequencing data, we found that the PERV copy numbers in Chinese pigs were lower (32.0±4.0) than in Western pigs (49.1±6.5). Additionally, the PERV sequence diversity was lower in Chinese pigs than in Western pigs. Regarding the lcPERV copy numbers, PERV-A and -JX2 in Western pigs were higher than in Chinese pigs. Notably, Bama Xiang (BMX) pigs had the lowest PERV copy number (27.8±5.1), and a BMX individual had no PERV-C and the lowest PERV copy number (23), suggesting that BMX pigs were more suitable for screening and/or modification as xenograft donors. Furthermore, we identified 451 PERV transposon insertion polymorphisms (TIPs), of which 86 were shared by all 10 Chinese and Western pig breeds. Our findings provide systematic insights into the genomic distribution, variation, evolution, and possible biological function of PERVs.
- Published
- 2022
- Full Text
- View/download PDF
17. Genetic variation of porcine prostaglandin-endoperoxide synthase 2 (PTGS2) gene and its association with reproductive traits in an Erhualian x Duroc F2 population.
- Author
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Ding NS, Ren DR, Guo YM, Ren J, Yan Y, Ma JW, Chen KF, and Huang LS
- Subjects
- Animals, Crosses, Genetic, Female, Gene Frequency, Genotype, Litter Size genetics, Male, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Pregnancy, Quantitative Trait Loci genetics, Reproduction genetics, Swine classification, Cyclooxygenase 2 genetics, Genetic Variation, Swine genetics
- Abstract
Identification of major genes that genetically impact female fertility is important for successful selection of high prolificacy pig lines. Because it is the rate-limiting enzyme in the conversion of arachidonic acid to prostaglandins (PGs), which are important for ovulation, fertilization, implantation, decidualization and parturition, prostaglandin-endoperoxide synthase 2 gene (PTGS2) is a potential candidate gene affecting porcine reproductive traits. In this study, a PCR-RFLP was used to genotype a total of 1 031 animals, including 661 from twelve Chinese local pig breeds, 190 from three Western pig breeds and 180 F2 sows from Nanchang pig resource family. Differences in frequency distributions of PTGS2 among twelve Chinese and three Western pig breeds and populations generally agree with their prolificacy. The allele frequencies in Lower Changjiang River Basin Type pig breeds, North China Type and Central China Type breeds are significantly different from those in South China Type, Plateau Type and Western pig breeds (P<0.001). And no significant differences were observed among Lower Changjiang River Basin Type, North China Type, Central China Type pig breeds, between South China Type and Western pig breeds, in part because of similar fertility patterns. And notable associations as well as reliable additive and dominant effects were not detected in an Erhualian x Duroc F2 population (P>0.05). Whereas, there is a trend for animals with one copy of the favourable A allele to have an increased TNB (total number of piglet born) and TBA (the number of piglets born alive) and a decreased SB (stillborn pigs) trait. Considering its crucial role in reproductive pathways, the PTGS2 gene deserves further study.
- Published
- 2006
- Full Text
- View/download PDF
18. [Generation of Chicken Germ-line Chimeras by Transferring PGCs and Their Identification by AFLP.].
- Author
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Hu XF, Xie B, Yu RS, Huang QZ, Zhang DF, Huang LS, and Li Z
- Subjects
- Amplified Fragment Length Polymorphism Analysis, Animals, Cells, Cultured, Embryonic Development, Germ Cells, Chickens genetics, Chimera
- Abstract
PGCs (Primordial germ cells) were isolated from the blood of 51~56 h hatching Shiqiza chicken embryos by Ficoll density gradient centrifugation. The PGCs were injected into 2.5 d hatching embryos of H breed chicken to produce germ-line chimeras. AFLP checking method was established to identify chicken germline chimeras. Eight germ-line H-S chimera embryos were identified among 20 developing H breed embryos.
- Published
- 2005
19. [Studies of microsatellite isolation from porcine PAC clone].
- Author
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Gao J, Ren J, Chen KF, and Huang LS
- Abstract
A novel microsatellite DNA was isolated from a single porcine PAC clone by sequencing the PAC clone directly with (CA)n repeat motif anchored primer. The specific primer pairs flanking the (CA)n repeat region were used to amplify the genomic DNA of 40 individuals from 8 pig breeds, which detected three alleles with the fragment length of 305 bp, 307 bp and 309 bp. The PCR product sequencing results of homozygous animals representing three alleles revealed that those three alleles contained 12,13 and 14 CA dinucleotide repeats respectively.
- Published
- 2003
20. [Studies of the relationship of melanocortin receptor 1(MC1R) gene with coat color phenotype in pigs].
- Author
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Deng SH, Gao J, Ren J, Chen KF, Ding NS, Ai HS, Lin WH, Wang WJ, Liu BS, Lai FJ, and Huang LS
- Subjects
- Alleles, Animals, Breeding, DNA genetics, DNA isolation & purification, Female, Gene Frequency, Genotype, Male, Pedigree, Phenotype, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Polymorphism, Single-Stranded Conformational, Receptor, Melanocortin, Type 1 genetics, Skin Pigmentation genetics, Swine genetics
- Abstract
Although coat color in pigs has no direct relation with economic traits, it affects economic benefit significantly, coat color selection are widely used in pig breeding and production. PCR-Acc II-RFLP, PCR-BspH I-RFLP and PCR-SSCP were used in combination to analyze genotype at MC1R locus among individuals from 16 full-sib pedigrees and 6 Chinese native breeds including Jinhua, Jiaxing Black, Yushan Black, Leping Spotted, Shanggao Spotted and Shengxian Spotted pig. It was found that the Chinese native pig breeds carry a dominant black allele at MC1R at high frequency, this ED1 allele was suggested to be the major allele controlling black coat color in Chinese native pig breed. In addition, the evidence for a new allele was obtained in Shengxian Spotted pigs by PCR-SSCP analysis. It was reconfirmed from the result of pedigree analysis that ED1 was dominant over EP and e, while EP was incompletely dominant over e.
- Published
- 2003
21. Research on the genetic variations of a1-fucosytransferase (FUT1) gene in 26 pig breeds.
- Author
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Yan XM, Ren J, Guo YM, Ding NS, Chen KF, Gao J, Ai HS, Chen CY, Ma JW, and Huang LS
- Subjects
- Alleles, Animals, Breeding, DNA genetics, Escherichia coli growth & development, Female, Gene Frequency, Genetic Variation, Genotype, Immunity, Innate genetics, Male, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Swine microbiology, Galactoside 2-alpha-L-fucosyltransferase, Fucosyltransferases genetics, Swine genetics
- Abstract
Enterotoxigenic Escherichia coli F18(ECF18) is a main pathogen that causes edema disease and post-weaning diarrhoea in piglets, and al-fucosytransferase (FUT1) gene has been identified as a candidate gene for controlling the expression of the receptor for ECF18 bacteria. The genetic variations at position 307 nucleotide in open reading frame of FUT1 gene in 26 pig breeds (total 1458 individuals) from 5 western commercial pig breeds and 21 Chinese native pig breeds were investigated by PCR-RFLP. The results showed that the genetic polymorphisms of the FUT1 locus were only detected in 5 western pig breeds and the Chinese Lingao pig breed, 5 western pig breeds possessed 3 different genotypes, and Lingao pig breed had two susceptible genotypes GG and AG, while all the other 20 Chinese native pig breeds only presented the susceptible genotype GG. The results indicated that if M307G-A point mutation in the coding region of FUT1 gene was the key factor determining the expression of the ECF18 receptor, most of Chinese native pig breeds were absent of the genetic background on the resistance to ECF18 bacteria. In this case, it was inferred that the resistance gene to ECF18 might be originated from western pig breeds. In addition, it is of great importance for the conservation of Lingao pig breed as it is the only found Chinese native pig breed possessing resistance M307A allele in FUT1 gene. Generally, compared with exotic pig breeds, Chinese native pig breeds have stronger resistance to edema disease and post-weaning diarrhoea in piglets. The results suggested that further study should be done to identify and characterize putative QTL (quantitative trait locus) or/and the functional gene responsible for the resistance to ECF18 in Chinese native pig breeds.
- Published
- 2003
22. [Polymorphism analysis of porcine myogenin gene by PCR-RFLP].
- Author
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Lin WH, Gao J, Chen KF, Ding NS, Ai HS, Guo YM, Li L, and Huang LS
- Abstract
The polymorphisms of porcine myogenin gene in 561 pigs including Duroc, Landrace, Large Yorkshire, Nanchang white pig, Erhualian, Meishan, Yushan black pig, Leping spotted pig, Jinhua black head-hind pig and Shanggao black head-hind pig were detected by PCR-RFLPs with three different pairs of primers,and the PCR products were digested by MspI. The results showed that most of the Duroc, Landrace, Large Yorkshire, Nanchang white pigs presented as AA genotype, while more animals of the six Chinese local pig breeds except for Leping spotted pig presented as BB genotype at PCR1 MspI-RFLP site. The six Chinese local breeds presented as MM genotype except that one Yushan black pig presented as MN genotype, while more swines of the exotic breeds including Duroc, Landrace, Large Yorkshire presented as NN genotype, and Nanchang white pig appeared to be closer to the exotic breeds at PCR2 MspI-RFLP site. PCR product was obtained in all the swine by PCR3, but the MspI restriction site was not found in the tested pig breeds including Meishan and blood closely related Erhualian pig.
- Published
- 2003
23. [Studies of population genetic relationships among 24 Chinese and exotic pig breeds using AFLP analysis].
- Author
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Ren J, Huang LS, Ai HS, Gary E, Gao J, Chen KF, Ding NS, and Deng SH
- Subjects
- Animals, Breeding, DNA genetics, DNA metabolism, Deoxyribonuclease EcoRI metabolism, Deoxyribonucleases, Type II Site-Specific metabolism, Female, Male, Phylogeny, Random Amplified Polymorphic DNA Technique, Swine classification, Genetics, Population, Swine genetics
- Abstract
A total of 12 AFLP primer combinations were used to detect genetic variation of pooled DNA in a sample of 19 Chinese native pig breeds, 1 cultivated pig breed and 4 European and American pig breeds. The genetic similarity coefficient of 24 pig beeds was calculated from AFLP data, UPGMA cluster analysis was also performed. The 12 primer combinations generated more than 1000 bands, of which 208 bands were polymorphic, 17.3 polymorphic markers were detected by one primer combination on the average. Thirteen putative breed specific bands were produced in the pooled DNA of 8 pig breeds. The cultivated pig breed and 4 exotic pig breeds were clustere into one group, while 19 Chinese native pig breeds were gathered into the other group in the UPGMA tree. The result indicated that AFLP analysis had high assay efficiency index (Ai) and provided a valuable tool for assaying genetic diversity and breed characterization in pigs. Chinese native pig breeds and exotic pig breeds show remarkable genetic differentiation, which had farther genetic relationships. Nanchang White pig and Large White pig, Yushan Black pig and Yanshan Black pig had intimate genetic relationships with each other respectively, which were consistent with its breeding history, geographical distribution and RAPD analysis results. In addition, the reasons for cluster results of some pig breeds from AFLP data were not consistent with morphology, geographical distribution and existing classification were discussed.
- Published
- 2002
24. [Relationship of growth hormone (GH 2) genotypes with some production performances in pig].
- Author
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Wang WJ, Chen KF, Ren J, Ding NS, Lin WH, Gao J, Ai HS, and Huang LS
- Subjects
- Adipose Tissue physiology, Animals, Birth Weight physiology, Genotype, Growth Hormone physiology, Polymerase Chain Reaction methods, Polymorphism, Restriction Fragment Length, Quantitative Trait, Heritable, Swine, Weight Gain physiology, Adipose Tissue growth & development, Birth Weight genetics, Growth Hormone genetics, Weight Gain genetics
- Abstract
The genotypes of 117 Nanchang White pigs and 361 Large Yorkshire pigs at GH 2 locus were detected by PCR-RFLP. The PCR products were cut by Apa I, and produced two alleles: A(449 + 101 + 55 bp), and B(316 + 133 + 101 + 49 bp). Effects of different genotypes on some important production traits involving the birth weight, 2-month body weight, 6-month body weight, corrected back-fat thickness, average back-fat thickness, feed to gain ratio and lean percentage were analyzed. The results showed that in Nanchang White pigs, no significant differences were observed between different genotypes and different growth and carcass traits; while in Large Yorkshire, the pigs with BB genotype had more lean percentage than pigs with AA genotype (P < 0.05).
- Published
- 2002
25. [Research on genetic variation of heart fatty acid-binding protein gene in ten pig breeds].
- Author
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Lin WH, Huang LS, Ren J, Deng SH, Wang WJ, Liu BS, Zhou LH, and Chen CY
- Subjects
- Animals, Animals, Outbred Strains genetics, Fatty Acid-Binding Proteins, Genotype, Polymerase Chain Reaction, Polymorphism, Genetic, Restriction Mapping, Carrier Proteins genetics, Genetic Variation, Myocardium metabolism, Neoplasm Proteins, Swine genetics
- Abstract
The genetic variation of heart fatty acid-binding protein (H-FABP) gene in 561 pigs including Duroc, Landrace, Large Yorkshire, Nanchang white pig, Erhualian, Meishan, Yushan black pig, Leping spotted pig, Jinhua black head-hind pig and Shanggao black head-hind pig were detected by PCR-RFLP with Hinf I, Hae III and Msp I. The results showed as follows: (1) Nanchang white pig presented only HH genotype while other breeds varied at the Hinf I-RFLP site; (2) The exotic breeds including Duroc, Landrace, Large Yorkshire and the native breed Nanchang white pig were proved to be polymorphic while the five Chinese local breeds presented no variation at the Hae III-RFLP site; (3) Among the tested breeds only Duroc presented variation at the Msp I-RFLP site. It is noted that all the Chinese local breeds present as AADD--genotypes.
- Published
- 2002
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