Search

ObjectiveTo provide reference for the disposition and clinical treatment of poisoning incidents， rapid toxin screening and the clinical symptoms and treatment of a case of Gymnopilus dilepis poisoning were conducted and discussed.MethodsUltra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry was used to detect the remaining poisonous mushroom in the contaminated food. Based on an epidemiological investigation， case data were collected. The results of the epidemiological investigation and the liver and kidney indexes of the patients were analyzed.ResultsPsilocybin and muscarine were detected in the Gymnopilus dilepis consumed by the patients. The symptoms were similar to those of psilocybin and muscarine poisoning， which included hallucinations， nausea， vomiting， abdominal pain， and diarrhea. The liver and kidney functions were normal.ConclusionThe poisoning event was caused by psilocybin and muscarine in Gymnopilus dilepis.

No consensus has been reached on the dysbiosis signs of microbiota in patients with urinary tract infections (UTIs). This meta-analysis aimed to verify the relationship between microbiota levels and UTIs. PubMed, Web of Science, and Embase databases were retrieved for related articles published from inception until October 20, 2021. The standardized mean difference (SMD) and its related 95% confidence intervals (CIs) of the microbiota diversity and abundance were pooled under a random-effects model. Twelve studies were included in this meta-analysis. The pooled analysis revealed that the microbiota diversity was lower in patients with UTIs than in healthy individuals (SMD = −0.655, 95% CI = −1.290, −0.021, I 2 = 81.0%, P = 0.043). The abundance of specific bacteria was higher in UTI subjects compared with healthy control individuals (SMD = 0.41, 95% CI = 0.07–0.74, P = 0.017), especially in North America patients with UTIs. Similar results were also found in studies with the total sample size being greater than 30. Importantly, Escherichia coli levels were increased in patients with UTI, whereas Lactobacillus levels decreased. E. coli and Lactobacilli have huge prospects as potential microbiota markers in the treatment of UTIs.

ObjectiveTo establish a rapid qualitative and quantitative method for the determination of 8 bufo toxins in toad skin residual from poisoned patients by ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry （UPLC/Q-TOF-MS）.MethodsAfter the toad skin samples were pretreated， the sample solution was separated on A Cquity UPLC® HSS T3 column （100 mm×2.1 mm， 1.8 μm） with 0.1% formic acid solution as mobile phase A and methanol as mobile phase B. The flow rate was 0.3 mL/min， the column temperature was 35 ℃， and the sample volume was 2 μL.ResultsThe linear ranges were 10.0-1 000.0 μg/L with r2≥0.990. The limits of detection were 0.7-2.1 μg/kg， and the limits of quantification were 2.5-7.0 μg/kg. The average recoveries were ≥75.79% and RSDs were ≤9.84%. The contents of bufalin， desacetylcinobufagin， desacetylcinobufotalin， pseudobufarenogin and bufotaline were 123.8±3.1， 5 920.4±1.9， 680.7±3.2， 671.6±9.1 and 1 796.6±74.9 μg/kg， respectively， while gamabufotain， cinobufagin and cinobufotalin were not detected.ConclusionThe method is practical， rapid and reliable for the determination of cinobufagin， bufalin， pseudobufarenogin， desacetylcinobufotalin and desacetylcinobufagin， bufotaline， gamabufotain and cinobufotalin in toad skin.

BackgroundThe prevalence of depressive disorder in adolescents is increasing. Adolescents with depressive disorder in remote counties and their families have a low awareness of the disease, and often are difficult to complete the whole treatment.ObjectiveTo explore the application effect of knowledge-to-action (KTA) framework-based health management in adolescents with depressive disorder in remote counties.MethodsNinety-four adolescents with depressive disorder coming from remote countries were recruited from the Second Affiliated Hospital of Nanchang University from June to December 2020. They were randomly divided into a routine group (receiving a 12-week routine health management) and a KTA group (receiving a 12-week KTA framework-based health management) . At the time of enrollment and 12 weeks after intervention, the status of non-suicidal self-injury was assessed by the Adolescent Non-suicidal Self-injury Assessment Questionnaire (ANSAQ) , mobile phone use was evaluated by the Self-rating Questionnaire for Adolescent Problematic Mobile Phone Use (SQAPMPU) , the anxiety was evaluated with the score of the Screen for Child Anxiety Related Emotional Disorders (SCARED) , and the depression was assessed by the Depression Self-Rating Scale for Childhood (DSRS) .ResultsTwo groups had no significant differences in mean total scores of ANSAQ, SQAPMPU, SCARED, and DSRS before receiving intervention (P>0.05) .Two groups had no significant differences in each dimension score of SQAPMPU before receiving intervention (P>0.05) .After the 12-week intervention, the mean total scores of ANSAQ, SQAPMPU, SCARED, and DSRS decreased significantly in both groups (P

Uterine fibroids are the most common benign tumors of the uterus. They primarily occur in women aged 30-50 years old, rarely in adolescents. In this article, the diagnosis and treatment of a 16-year-old teenager with recurrent submucosal fibroids were reported. The patient underwent hysteroscopic myomectomy due to abnormal uterine bleeding （AUB）, whereas failed to receive postoperative follow-up on a regular basis. At 18 months after the operation, she was admitted to hospital again due to AUB. Transvaginal ultrasound detected a space-occupying mass of 5.2 cm×4.6 cm×3.8 cm in the uterine cavity. She repeatedly underwent hysteroscopic partial myomectomy. Postoperative pathological biopsy indicated the diagnosis of submucosal uterine fibroids. The diagnosis and treatment of this case suggests that in the diagnosis and treatment of recurrent uterine fibroids in adolescents, it is necessary to differentiate fibroids from sarcomatosis. Meantime, the patient’s fertility should be preserved to the largest extent and postoperative follow-up should be strengthened.

Aims: This study aimed to investigate the impact of the triglyceride‐glucose index (TyG index) on clinical consequences in individuals with large vascular occlusion (LVO)‐induced acute ischemic stroke (AIS) following endovascular treatment (EVT). Methods: We conducted a single‐center retrospective cohort study, including AIS with LVO who underwent EVT. Patients were categorized into TyG index groups, calculated as "(fasting triglyceride [mg/dL] × fasting blood glucose [mg/dL]/2)." Clinical outcomes were assessed, including poor outcome (modified Rankin Scale [mRS] > 2 [3–6]) at 90 days, early neurological deterioration (END), symptomatic intracranial hemorrhage (sICH), and 90‐day mortality after EVT. Logistic regression and restricted cubic splines (RCS) were used to examine the relationship between the TyG index and clinical outcomes. Receiver operating characteristic (ROC) curve was constructed to evaluate the prognostic capacity of the TyG index. Results: A total of 424 patients were included. Higher TyG levels were associated with worse functional outcome at 90 days (per unit: p = 0.006), sICH (per unit: p = 0.002, T3 versus T1: p = 0.004), and 90‐day mortality (T2 versus T1: p = 0.011, T3 versus T1: p = 0.029) in logistic regression. A RCS model revealed a linear association between the TyG index and poor outcome at 90 days, sICH, and 90‐day mortality (p for nonlinearity > 0.05). In ROC curve analysis, the traditional risk factors model (area under the curve [AUC]: 0.824, 95% CI: 0.784–0.859) was outperformed by the conventional risk factors + TyG index model (AUC: 0.845, 95% CI: 0.807–0.878) in predicting poor outcome (p = 0.021). Conclusion: A higher TyG index is associated with worse clinical outcomes in LVO‐induced AIS patients after EVT. Additionally, the TyG index enhances risk prediction of traditional risk factors for poor outcome. [ABSTRACT FROM AUTHOR]

To investigate the molecular mechanisms of tea plants in response to low temperature stress, this study simulated the spring chill temperature pattern, using 'Fuding Dabaicha' as the experimental material for transcriptome sequencing, and subjected it to varying low temperature treatments. Differentially expressed genes (DEGs) were analyzed using the GO and KEGG pathway databases for metabolic pathway enrichment analysis, which revealed that these genes were mainly enriched in plant cell wall and metabolic pathways related to photosynthesis. Subsequently, thirteen DEGs were selected for validation via real-time quantitative PCR (qPCR), confirming the consistency of the qPCR results with the transcriptome sequencing data, thereby validating the reliability of the transcriptome data. In a subsequent study, two tea cultivars, 'Fuding Dabaicha' and 'Shuchazao', were used to evaluate various physiological indices, including leaf tissue structure, the contents of various cell wall components (cellulose, hemicellulose, and pectin), chlorophyll content, and chlorophyll fluorescence parameters. The results indicate that the leaf tissue structures of both tea cultivars underwent different degrees of thickening in response to low-temperature stress. Notably, significant differences were observed in the contents of cellulose and hemicellulose between the two cultivars, whereas the pectin content change was less pronounced. Furthermore, the chlorophyll content, photochemical quenching coefficient, maximum photochemical efficiency, and relative electron transport rate all exhibited a downward trend. Conversely, the non-photochemical quenching coefficient showed an upward trend. These observations highlight the key role of changes in cell wall components, particularly hemicellulose, and changes in photosynthesis-related parameters in the tea plants' response to low temperature. [ABSTRACT FROM AUTHOR]

Background: Hypertension‐induced impairment of the cerebral artery network contributes to cognitive impairment. Characterizing the structure and function of cerebral arteries may facilitate the understanding of hypertension‐related pathological mechanisms and lead to the development of new indicators for cognitive impairment. Purpose: To investigate the associations between morphological features of the intracranial arteries distal to the circle of Willis on time‐of‐flight MRA (TOF‐MRA) and cognitive performance in a hypertensive cohort. Study Type: Prospective observational study. Population: 189 hypertensive older males (mean age 64.9 ± 7.2 years). Field Strength/Sequence: TOF‐MRA sequence with a 3D spoiled gradient echo readout and arterial spin labeling perfusion imaging sequence with a 3D stack‐of‐spirals fast spin echo readout at 3T. Assessment: The intracranial arteries were segmented from TOF‐MRA and the total length of distal arteries (TLoDA) and number of arterial branches (NoB) were calculated. The mean gray matter cerebral blood flow (GM‐CBF) was extracted from arterial spin labeling perfusion imaging. The cognitive level was assessed with short‐term and long‐term delay‐recall auditory verbal learning test (AVLT) scores, and with montreal cognitive assessment. Statistical Tests: Univariable and multivariable linear regression were used to analyze the associations between TLoDA, NoB, GM‐CBF and the cognitive assessment scores, with P < 0.05 indicating significance. Results: TLoDA (r = 0.314) and NoB (r = 0.346) were significantly correlated with GM‐CBF. Multivariable linear regression analyses showed that TLoDA and NoB, but not GM‐CBF (P = 0.272 and 0.141), were significantly associated with short‐term and long‐term delay‐recall AVLT scores. These associations remained significant after adjusting for GM‐CBF. Data Conclusion: The TLoDA and NoB of distal intracranial arteries on TOF‐MRA are significantly associated with cognitive impairment in hypertensive subjects. Level of Evidence: 2 Technical Efficacy: Stage 3 [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate the neural plasticity in contralesional cortex and the effects of tongxinluo (TXL) in cerebral ischemic rats.