Search

Your search keyword '"Huang, Yongheng"' showing total 135 results
Start Over Author "Huang, Yongheng"
135 results on '"Huang, Yongheng"'

20. Targeted next-generation sequencing extends the mutational spectrums for OPA1 mutations in Chinese families with optic atrophy

Author

Wang, Yuwei, Xu, Min, Liu, Xiaoxing, Huang, Yongheng, Zhou, Yao, Liu, Qinghuai, Chen, Xue, Zhao, Chen, and Wang, Min

Subjects
Adult, Male, Adolescent, Fundus Oculi, High-Throughput Nucleotide Sequencing, Middle Aged, eye diseases, GTP Phosphohydrolases, Pedigree, Asian People, Mutation, Optic Atrophy, Autosomal Dominant, Humans, Family, Child, Research Article
Abstract

Purpose We aim to reveal the disease-causing mutations in 15 Chinese families with optic atrophy (OA). Methods In total, 15 families with OA were recruited in the present study. Medical histories were carefully reviewed and comprehensive ophthalmic examinations were received by all recruited patients. Targeted next-generation sequencing (NGS) was selectively performed on all probands for mutation detection. Intrafamilial cosegregation and in-silico analyses were subsequently applied to predict the potential pathogenic effects of identified mutations. Results All included patients presented bilateral vision loss. Their fundus photographs showed temporal or total pallor of the optic discs. Fourteen mutations in the optic atrophy 1 (OPA1) gene were revealed as disease-causing mutations for the 15 families, including eight novel (c.968A>G, c.193C>G, c.1071dupT, c.987_988del, c.2012+2T>G, c.1036–1G>C, c.2126A>G, and c.1036_1038del) and six recurrent (c.1499G>A, c.1800C>A, c.1034G>A, c.2873_2876del, c.112C>T, and c.804_805del) mutations. Conclusions In conclusion, our study expands the mutational spectrum for the OPA1 gene and implies targeted NGS as an effective approach for the genetic diagnosis of OA, which might help to improve the clinical diagnosis for patients with OA.

Published
2019

22. An Efficient Chemical-Defined Condition for Generation of Human Induced Pluripotent Stem Cells

Author

Fang Shi, shao zhicheng, Liu Huihui, Li Bo, Wang Naweng, Huang Yongheng, Fan Qi, Xu Jinhong, and Shi Jingyi

Subjects
Chemistry, Human Induced Pluripotent Stem Cells, Cell biology
Abstract

Background: Human induced pluripotent stem cells (hiPSCs) hold great potential in disease modeling, drug screening and cell therapy. However, efficiency and costs of hiPSCs preparation still need to be improved.Methods: We screened the compounds that target signaling pathways, epigenetic modifications or metabolic-process regulation to replace the growth factors. After small molecules treatment, TRA-1-60 staining was performed to quantify the efficiency of somatic cell reprogramming. Next, small molecule cocktail induced ESCs or iPSCs were examined with pluripotent markers expression. Finally, Genome-wide gene expression profile was then analyzed by RNA-seq to illustrate the mechanism of human somatic cell reprogramming. Result: Here, we found that a dual-specificity tyrosine phosphorylation-regulated kinase inhibitor ID-8 robustly enhanced human somatic cell reprogramming by upregulation of PDK4 and activation of glycolysis. Furthermore, we identified a novel growth-factor-free hiPSC generation system using small molecules ID-8/Kartogenin (IK). Finally, we developed IK medium combined with Low-dose bFGF to support the long-term expansion of human pluripotent stem cells. IK-iPSCs showed pluripotency and normal karyotype. Conclusions: Our studies may provide a novel growth-factor-free culture system to facilitate the generation of hiPSCs for multiple application in regenerative medicine.

Published
2021

23. Alterations in the Retinal Vascular Network and Structure in Myelin Oligodendrocyte Glycoprotein Antibody-Associated Optic Neuritis: A Longitudinal OCTA Study.

Author

Yu, Jian, Huang, Yongheng, Wu, Kaicheng, ZhangBao, Jingzi, Zhou, Lei, Zong, Yuan, Zhou, Xujiao, Quan, Chao, and Wang, Min

Subjects
*MYELIN oligodendrocyte glycoprotein, *OPTIC neuritis, *RETINAL blood vessels, *LONGITUDINAL method
Abstract

To investigate the longitudinal microstructural and microvascular changes in patients with myelin oligodendrocyte glycoprotein antibody-associated optic neuritis (MOG-ON) without new attacks. We included 20 eyes of 12 MOG-ON patients without new attacks during the follow-up and 24 eyes of 12 age- and sex-matched healthy controls. The BCVA, retinal vessels and structure were significantly lower in MOG-ON eyes than in healthy eyes(all P <.05). In MOG-ON eyes, the BCVA (p =.408) and mean deviation (p =.854) were not significantly decreased at the follow-up visit. However, there were small, significant decreases in parafoveal vessel density (p =.026), peripapillary vessel density (p =.008), and RNFL thickness (p =.03), but not GCIPL thickness (p =.107). Ongoing deterioration was observed in RNFL thickness and parafoveal and peripapillary vessel density, but not GCIPL thinning, in MOG-ON eyes without a new attack of ON. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

27. Alterations in the Retinal Vascular Network and Structure in MOG Antibody-Associated Disease: An Optical Coherence Tomography Angiography Study.

Author

Yu, Jian, Huang, Yongheng, Quan, Chao, Zhou, Lei, ZhangBao, Jingzi, Wu, Kaicheng, Zong, Yuan, Zhou, Xujiao, and Wang, Min

Abstract

Supplemental Digital Content is Available in the Text. Background: To determine retinal vessel density in patients with myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD). Methods: Twenty-five patients with MOGAD and 20 healthy participants were enrolled. Patients with MOGAD were divided into myelin oligodendrocyte glycoprotein antibody (MOG-Ab)-positive eyes with a history of optic neuritis (ON; MOG-Ab-ON+ group) or without a history of ON (MOG-Ab-ON− group). Visual function, retinal vessel densities, and thickness were measured. Results: The retinal nerve fiber layer, parafoveal ganglion cell and inner plexiform layers, and vessel densities in the peripapillary and parafoveal areas were significantly decreased in the MOG-Ab-ON+ eyes compared with healthy eyes and MOG-Ab-ON− eyes (all P < 0.05). An increasing number of ON episodes was associated with greater decreases in these variables (all P < 0.05). Visual field mean deviation was not significantly decreased in patients with a history of 1 or 2 episodes of ON, although the relative decreases in retinal nerve fiber layer thickness, parafoveal ganglion cell and inner plexiform layer thickness, peripapillary vessel density, and parafoveal vessel density reached 33.1%, 23.2%, 17.0%, and 11.5% (all P < 0.05), respectively, in eyes with 2 episodes of ON. The mean deviation was significantly correlated with peripapillary vessel density (P < 0.05) after adjustment for other variables. Best-corrected visual acuity was not significantly correlated with optical coherence tomography variables (all P > 0.05). Conclusions: MOG-Ab-associated ON was associated with significant decreases in retinal structure and vessel density, without significant deteriorations in visual function. The peripapillary vessel density might predict the visual outcomes in patients with MOG-Ab-associated ON. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

36. Metal‐Organic Framework MIL‐125 Derived Mg2+‐Doped Mesoporous TiO2 for Photocatalytic CO2 Reduction.

Author

Feng, Xuhui, Pan, Fuping, Zhang, Peng, Wang, Xiao, Zhou, Hong‐Cai, Huang, Yongheng, and Li, Ying

Subjects
PHOTOREDUCTION, METAL-organic frameworks, CARBON dioxide adsorption, TITANIUM dioxide, CHARGE transfer, PHOTOCATALYSTS, PHOTOCURRENTS
Abstract

In this study, Mg2+‐doped mesoporous TiO2 photocatalysts derived from Mg2+ adsorption (MA) process on MIL‐125, a metal‐organic framework material, were prepared and employed for photocatalytic reduction of CO2 to produce CO. The Mg2+ doping concentration was controlled by varying the Mg2+ concentration in the Mg2+ adsorption process. It was demonstrated that the Mg2+ doping promoted the generation of surface Ti3+ and significantly increased transient photocurrent density. Over a 4 h UV/Vis irradiation period, the best performing photocatalyst, 1MA, delivered a CO production rate ∼20 times higher than that of P25, a commercially available TiO2 nanopowder. It is believed that the Mg2+ adsorption process introduced more favorable properties to the TiO2 photocatalysts, such as higher surface area and porosity for more reactive sites, and concentrated surface Ti3+ centers for improved charge transfer. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

37. Peripapillary and parafoveal vascular network assessment by optical coherence tomography angiography in aquaporin-4 antibody-positive neuromyelitis optica spectrum disorders

Author

Huang, Yongheng, primary, Zhou, Lei, additional, ZhangBao, Jingzi, additional, Cai, Tongjia, additional, Wang, Bei, additional, Li, Xiaoyang, additional, Wang, Liang, additional, Lu, Chuanzhen, additional, Zhao, Chongbo, additional, Lu, Jiahong, additional, Quan, Chao, additional, and Wang, Min, additional

Published
2018
Full Text
View/download PDF

39. Synergistic co-regulation and competition by a SOX9-GLI-FOXA phasic transcriptional network coordinate chondrocyte differentiation transitions

Author

Tan, Zhijia, primary, Niu, Ben, additional, Tsang, Kwok Yeung, additional, Melhado, Ian G., additional, Ohba, Shinsuke, additional, He, Xinjun, additional, Huang, Yongheng, additional, Wang, Cheng, additional, McMahon, Andrew P., additional, Jauch, Ralf, additional, Chan, Danny, additional, Zhang, Michael Q., additional, and Cheah, Kathryn S. E., additional

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results