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To ease the aging process of the population and change the situation that the fertility rate continues to decline, the Chinese government will adjust the two-child policy to the three-child policy in 2021. The implementation of the three-child policy can indeed release part of the reproductive potential, but childbirth will increase the employment cost of enterprises, which will lead to employment discrimination against women. My thesis is based on the comprehensive three-child policy. The main research problem is the Chinese government's handling of female employment discrimination and putting forward reasonable countermeasures to provide strong support for the Chinese government to deal with female employment discrimination. First of all, through the existing data, we can understand the current employment situation of women in China, and compare the employment situation of women with the employment situation of men. It mainly includes four aspects: high employment threshold, unequal pay for equal work, difficult promotion, and reduced maternity security. The reasons for the current employment discrimination of Chinese women are explained through Becker's model of individual discrimination. When the preference for discrimination is greater than the cost of discrimination, discriminatory behavior...

Zhaoming Huang, Panpan Yu, Jianhui Tang Department of Medical Cosmetology, Xianning Central Hospital, The First Affiliated Hosptial of Hubei University of Science and Technology, Xianning, Hubei 437000, People’s Republic of ChinaCorrespondence: Zhaoming HuangDepartment of Medical Cosmetology, Xianning Central Hospital, The First Affiliated Hosptial of Hubei University of Science and Technology, 228 Jingui Road, Xianning, Hubei 437000, People’s Republic of ChinaEmail 925172961@qq.comBackground: The tumor three-dimensional (3D) spheroid model in vitro is effective on detecting malignant cells and tumorigenesis, and assessing drug resistance. Compared with two-dimensional (2D) monolayer culture, breast cancer (BC) spheroids more accurately reflect the complex microenvironment in vivo, which have been extensively reported in BC research. MDA-MB-231 cells, the triple-negative breast cancer (TNBC) cell line, display representative epithelial to mesenchymal transition (EMT) associated with BC metastasis. However, the characterization of MDA-MB-231 spheroids has been largely unknown at present, which requires further attention.Materials and Methods: Microwell array was conducted for the formation of MDA-MB-231 spheroids. In addition, H&E staining, immunohistochemistry (IHC), CellTiter-Glo® 3D cell viability assay, and flow cytometry were performed to investigate the structure and growth characteristics. Besides, Transwell and scratch healing assays were carried out to detect the migratory capacities compared with 2D culture. Western blotting and confocal fluorescence were selected to detect the expression of EMT-associated proteins. Additionally, the half maximal inhibitory concentration (IC50) values of antitumor compounds Carboplatin and Doxorubicin were measured to assess drug resistance.Results: The MDA-MB-231 spheroids were viable, which maintained a compact structure with zonation features for up to 9 days. Moreover, those spheroids had a slower growth rate than those cultured as a monolayer and differential zones of proliferation. The migratory capacities were significantly enhanced by transferring the spheroids to 2D adherent culture. Compared with 2D culture, the levels of EMT-associated proteins were significantly up-regulated in spheroids. Furthermore, toxicity assessment showed that spheroids exhibited an increased resistance to the antitumor compounds.Conclusion: This study develops the simple spheroids and demonstrates their structure, growth and proliferation characteristics. According to our results, the spheroids are associated with superior EMT and high resistance to toxicological response compared with the standard 2D monocultures.Keywords: triple-negative breast cancer, spheroid, epithelial to mesenchymal transition, three-dimensional culture