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Your search keyword '"Huang, Zhi‐Guang"' showing total 329 results
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329 results on '"Huang, Zhi‐Guang"'

27. Construction of a panoramic mRNA map of adult noncystic fibrosis bronchiectasis and a preliminary study of the underlying molecular mechanisms.

Author

Huang, Wan-Ying, Hong, Kang-Kang, Luo, Jing, He, Rong-Quan, Huang, Zhi-Guang, Xu, Yang, Zhang, Chu-Yue, Bao, Chong-Xi, Zhang, Liang-Ming, Chen, Gang, and Kong, Jin-Liang

Subjects
GENE expression, POLYMERASE chain reaction, METABOLIC disorders, OXIDATIVE phosphorylation, C-reactive protein, BRONCHIECTASIS
Abstract

Background: The pathogenesis of noncystic fibrosis bronchiectasis in adults is complex, and the relevant molecular mechanisms remain unclear. In this study, we constructed a panoramic map of bronchiectasis mRNA, explored the potential molecular mechanisms, and identified potential therapeutic targets, thus providing a new clinical perspective for the preventive management of bronchiectasis and its acute exacerbation. Methods: The mRNA profiles of peripheral blood and bronchiectasis tissues were obtained through transcriptome sequencing and public databases, and bioinformatics methods were used to screen for differentially expressed genes (DEGs). The DEGs were then subjected to biological function and pathway analyses. Some DEGs were validated using a real-time quantitative polymerase chain reaction (RT-qPCR) in peripheral blood. Spearman's correlation analysis was used to analyse the correlation between DEGs and clinical indicators. Results: Based on transcriptome sequencing and public databases, the mRNA profile of bronchiectasis was determined. DEGs were obtained from the peripheral blood sequencing dataset (985 DEGs), tissue sequencing dataset (2919 DEGs), and GSE97258 dataset (1083 DEGs). Bioinformatics analysis showed that upregulated DEGs had enriched neutrophil-related pathways, and downregulated DEGs had enriched ribosome-related pathways. RT-qPCR testing confirmed the upregulated expression of VCAN, SESTD1, SLC12A1, CD177, IFI44L, SIGLEC1, and RSAD2 in bronchiectasis. These genes were related to many clinical parameters, such as neutrophils, C-reactive protein, and procalcitonin (P < 0.05). Conclusions: Transcriptomic methods were used to construct a panoramic map of bronchiectasis mRNA expression. The findings showed that neutrophil activation, chronic inflammation, immune regulation, impaired ribosomal function, oxidative phosphorylation, and energy metabolism disorders are important factors in the development of bronchiectasis. VCAN, SESTD1, SLC12A1, CD177, IFI44L, SIGLEC1, and RSAD2 may play important roles in the pathogenesis of bronchiectasis and are potential therapeutic targets. [ABSTRACT FROM AUTHOR]

Published
2024
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29. Nucleolar protein interacting with the FHA domain of MKI67 may be an important factor in promoting the development of colorectal cancer: a comprehensive study integrating bulk RNA-seq, scRNA-seq, protein immunohistochemistry and CRISPR

Author

Li, Hui, primary, Tang, Yu-Xing, additional, Chen, Yi, additional, Huang, Wan-Ying, additional, He, Rong-Quan, additional, Huang, Zhi-Guang, additional, Zeng, Da-Tong, additional, Xia, Zhi-Yuan, additional, Song, Rui, additional, Zhang, Han Jie, additional, Chen, Yu-Zhen, additional, Zhao, Bin-Hui, additional, Qin, Si-Ru, additional, Luo, Jia-Yuan, additional, and Chen, Gang, additional

Published
2024
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30. TM9SF1 promotes bladder cancer cell growth and infiltration

Author

Wei, Long, primary, Wang, Shi-Shuo, additional, Huang, Zhi-Guang, additional, He, Rong-Quan, additional, Luo, Jia-Yuan, additional, Li, Bin, additional, Cheng, Ji-Wen, additional, Wu, Kun-Jun, additional, Zhou, Yu-Hong, additional, Liu, Shi, additional, Li, Sheng-Hua, additional, and Chen, Gang, additional

Published
2024
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39. MMP12 serves as an immune cell–related marker of disease status and prognosis in lung squamous cell carcinoma

Author

Zhang, Wei, primary, Li, Guo-Sheng, additional, Gan, Xiang-Yu, additional, Huang, Zhi-Guang, additional, He, Rong-Quan, additional, Huang, Hong, additional, Li, Dong-Ming, additional, Tang, Yu-Lu, additional, Tang, Deng, additional, Zou, Wen, additional, Liu, Jun, additional, Dang, Yi-Wu, additional, Chen, Gang, additional, Zhou, Hua-Fu, additional, Kong, Jin-Liang, additional, and Lu, Hui-ping, additional

Published
2023
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43. High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistry.

Author

Jiang, Fang‐Cheng, Zhai, Gao‐Qiang, Liu, Jia‐Lin, Wang, Rui‐Gong, Yang, Yuan‐Ping, Murugesan, Harivignesh, Yu, Xiao‐Xiang, Du, Xiu‐Fang, He, Juan, Feng, Zhen‐Bo, Pan, Shang Ling, Chen, Gang, Li, Sheng‐Hua, and Huang, Zhi‐Guang

Subjects
ANDROGEN receptors, PROSTATE cancer, DATA mining, CENTROMERE, GENE expression, CANCER stem cells, PROTEIN expression
Abstract

The progression of prostate cancer (PCa) leads to poor prognosis. However, the molecular mechanism of PCa is still not completely clear. This study aimed to elucidate the important role of centromere protein A (CENPA) in PCa. Large numbers of bulk RNA sequencing (RNA‐seq) data and in‐house immunohistochemistry data were used in analysing the expression level of CENPA in PCa and metastatic PCa (MPCa). Single‐cell RNA‐seq data was used to explore the expression status of CENPA in different prostate subpopulations. Enrichment analysis was employed to detect the function of CENPA in PCa. Clinicopathological parameters analysis was utilised in analysing the clinical value of CENPA. The results showed that CENPA was upregulated in PCa (standardised mean difference [SMD] = 0.83, p = 0.001) and MPCa (SMD = 0.61, p = 0.029). CENPA was overexpressed in prostate cancer stem cells (CSCs) with androgen receptor (AR) negative compared to epithelial cells with AR positive. CENPA may influence the development of PCa through affecting cell cycle. Patients with nodal metastasis had higher expression level of CENPA. And patients with high CENPA expression had poor disease‐free survival. Taken together, Overexpression of CENPA may influence the development of PCa by regulating cell cycle and promoting metastasis. [ABSTRACT FROM AUTHOR]

Published
2023
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46. Clinicopathological and prognostic significance of XPO1 in solid tumors: meta-analysis and TCGA analysis

Author

Tan, Yang, Chen, Gang, He, Rong-Quan, Huang, Zhi-Guang, Dang, Yi-Wu, Luo, Jia-Yuan, Huang, Wan-Ying, Huang, Su-Ning, Liu, Run, and Feng, Zhen-Bo

Abstract

Exportin 1 (XPO1) is overexpressed in several solid tumors, and is associated with poor prognosis. Here, we aimed to evaluate the implication of XPO1 expression in solid tumors through a meta-analysis. PubMed, Web of Science, and Embase databases were searched for articles published until February 2023. Statistical data of the patients, odds ratios and hazard ratios (HRs), together with their corresponding 95% confidence intervals (CIs) were pooled to assess clinicopathological features and survival outcomes. Besides, the Cancer Genome Atlas (TCGA) was used to explore the prognostic significance of XPO1 in solid tumors. A total of 22 works, comprising 2595 patients were included in this study. The results suggested that increased XPO1 expression was associated with a higher tumor grade, more lymph node metastasis, advanced tumor stage, and progressively worse total clinical stage. Additionally, high XPO1 expression was associated with worse overall survival (OS) (HR = 1.43, 95% CI = 1.12–1.81, P = 0.004) and shorter progression-free survival (HR = 1.40, 95% CI = 1.07–1.84, P = 0.01). An analysis using the TCGA dataset showed that high XPO1 expression was associated with poor OS and disease-free survival. XPO1 is a promising prognostic biomarker and may constitute a therapeutic target for solid tumors. PROSPERO registration number: CRD42023399159

Published
2023
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