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23 results on '"Huang, Zhong-Qin"'

7. Additional file 1 of MYB44 competitively inhibits the formation of the MYB340-bHLH2-NAC56 complex to regulate anthocyanin biosynthesis in purple-fleshed sweet potato

Author

Zeng-Zheng Wei, Kang-Di Hu, Zhao, Dong-Lan, Tang, Jun, Huang, Zhong-Qin, Jin, Peng, Li, Yan-Hong, Han, Zhuo, Hu, Lan-Ying, Gai-Fang Yao, and Zhang, Hua

Abstract

Additional file 1: Figure S1. Amino acid sequence alignment of IbNACs and the NAC transcription factor PpBL (ALK27819.1) in peach (Prunus persica). Table S1. List of primers used for RT-qPCR. Table S2. The list of primers used for developing the constructs. Table S3. Protein sequences subjected to phylogenetic analysis and multiple sequence alignments.

Published
2020
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11. Hydrogen Sulfide Inhibits Fruit Softening by Regulating Ethylene Synthesis and Signaling Pathway in Tomato (Solanum lycopersicum)

Author

Hu, Kang-Di, primary, Zhang, Xiao-Yue, additional, Wang, Sha-Sha, additional, Tang, Jun, additional, Yang, Feng, additional, Huang, Zhong-Qin, additional, Deng, Jing-Yu, additional, Liu, Si-Yuan, additional, Zhao, Shang-Jun, additional, Hu, Lan-Ying, additional, Yao, Gai-Fang, additional, and Zhang, Hua, additional

Published
2019
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20. Antioxidative system in sweet potato root is activated by low‐temperature storage.

Author

Wang, Si‐Qi, Hu, Kang‐Di, Li, Yan‐Hong, Yao, Gai‐Fang, Zhang, Hua, Hu, Lan‐Ying, Tang, Jun, Huang, Zhong‐Qin, Yang, Feng, and Zhang, Hui‐Yun

Subjects
SWEET potatoes, REACTIVE oxygen species, ANTIOXIDANTS, ENZYMES, METABOLITES
Abstract

BACKGROUND Sweet potato is susceptible to chilling injury during low‐temperature storage. To explore the correlation between chilling injury and reactive oxygen species (ROS) metabolism, the content of ROS and the activities and gene expression of antioxidant enzymes were analyzed in the typical storage‐tolerant cultivar Xushu 32 and storage‐sensitive cultivar Yanshu 25. RESULTS: The activities of antioxidant enzymes including ascorbate peroxidase (APX), superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were enhanced rapidly in the early period of storage in response to chilling stress. Thereafter, the content of ROS metabolites increased consistently due to gradual decrease in ROS scavenging enzymes. Storage‐tolerant cultivar Xushu 32 had higher antioxidant enzyme activities and gene expressions as well as higher content of antioxidant metabolites and lower content of ROS metabolites compared with storage‐sensitive cultivar Yanshu 25, suggesting that the capacity of ROS scavenging by antioxidant enzymes and antioxidants is highly associated with the tolerance of sweet potato to chilling stress. CONCLUSION: These results indicated that the antioxidative system is activated in the storage root of sweet potato and the antioxidative capacity is positively associated with better storage performance in the storage‐tolerant cultivar. © 2019 Society of Chemical Industry [ABSTRACT FROM AUTHOR]

Published
2019
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21. Functional Characterization of a Cystathionine β-Synthase Gene in Sulfur Metabolism and Pathogenicity of Aspergillus nigerin Pear Fruit

Author

Guo, Shang-Xuan, Yao, Gai-Fang, Ye, Hui-Ran, Tang, Jun, Huang, Zhong-Qin, Yang, Feng, Li, Yan-Hong, Han, Zhuo, Hu, Lan-Ying, Zhang, Hua, and Hu, Kang-Di

Abstract

Aspergillus niger, which is a fungal pathogen, causes rot in a variety of fruits. In this study, the cystathionine β-synthase cbsAgene was deleted by homologous recombination to study its role in sulfur metabolism and pathogenicity of A. niger.The results showed that ΔcbsAstrain maintained normal mycelia growth and sporulation compared with the control strain A. nigerMA 70.15, whereas the contents of cysteine and glutathione (GSH) increased significantly after cbsAdeletion. However, ΔcbsAstrain showed reduced endogenous H2S production. Further results showed that cbsAgene deletion induced higher resistance to cadmium stress and stronger infectivity to pears. It was also found that a stronger response of reactive oxygen species (ROS) production was induced in ΔcbsAmutant-infected pear compared with the control strain. In all, the present research suggested the important role of cbsAin sulfur metabolism and pathogenicity of A. nigerin pear fruit.

Published
2019
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23. Reduction of Aspergillus nigerVirulence in Apple Fruits by Deletion of the Catalase Gene cpeB

Author

Zhang, Meng-Ke, Tang, Jun, Huang, Zhong-Qin, Hu, Kang-Di, Li, Yan-Hong, Han, Zhuo, Chen, Xiao-Yan, Hu, Lan-Ying, Yao, Gai-Fang, and Zhang, Hua

Abstract

Aspergillus niger, a common saprophytic fungus, causes rot in many fruits. We studied the role of a putative catalase-peroxidase-encoding gene, cpeB, in oxidative stress and virulence in fruit. The cpeBgene was deleted in A. nigerby homologous recombination, and the ΔcpeBmutant showed decreased CAT activity compared with that of the wild type. The cpeBgene deletion caused increased sensitivity to H2O2stress, and spore germination was significantly reduced; in addition, the reactive-oxygen-species (ROS) metabolites superoxide anions (·O2–), hydrogen peroxide (H2O2), and malondialdehyde (MDA) accumulated in the ΔcpeBmutant during H2O2stress. Furthermore, ROS metabolism in A. nigerinfected apples was determined, and our results showed that the ΔcpeBmutant induced an attenuated response in apple fruit during the fruit–pathogen interaction; the cpeBgene deletion significantly reduced the development of lesions, suggesting that the cpeBgene in A. nigeris essential for full virulence in apples.

Published
2018
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