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43 results on '"Huang Lien-Hung"'

2. Identification of Crucial Cancer Stem Cell Genes Linked to Immune Cell Infiltration and Survival in Hepatocellular Carcinoma.

Author

Huang, Lien-Hung, Wu, Shao-Chun, Liu, Yueh-Wei, Liu, Hang-Tsung, Chien, Peng-Chen, Lin, Hui-Ping, Wu, Chia-Jung, Hsieh, Ting-Min, and Hsieh, Ching-Hua

Subjects
*DNA topoisomerase II, *CANCER stem cells, *LEUCINE zippers, *HOLLIDAY junctions, *GENE regulatory networks, *FORKHEAD transcription factors, *CELL cycle regulation
Abstract

Hepatocellular carcinoma is characterized by high recurrence rates and poor prognosis. Cancer stem cells contribute to tumor heterogeneity, treatment resistance, and recurrence. This study aims to identify key genes associated with stemness and immune cell infiltration in HCC. We analyzed RNA sequencing data from The Cancer Genome Atlas to calculate mRNA expression-based stemness index in HCC. A weighted gene co-expression network analysis was performed to identify stemness-related gene modules. A single-sample gene set enrichment analysis was used to evaluate immune cell infiltration. Key genes were validated using RT-qPCR. The mRNAsi was significantly higher in HCC tissues compared to adjacent normal tissues and correlated with poor overall survival. WGCNA and subsequent analyses identified 10 key genes, including minichromosome maintenance complex component 2, cell division cycle 6, forkhead box M1, NIMA-related kinase 2, Holliday junction recognition protein, DNA topoisomerase II alpha, denticleless E3 ubiquitin protein ligase homolog, maternal embryonic leucine zipper kinase, protein regulator of cytokinesis 1, and kinesin family member C1, associated with stemness and low immune cell infiltration. These genes were significantly upregulated in HCC tissues. A functional enrichment analysis revealed their involvement in cell cycle regulation. This study identified 10 key genes related to stemness and immune cell infiltration in HCC. These genes, primarily involved in cell cycle regulation, may serve as potential targets for developing more effective treatments to reduce HCC recurrence and improve patient outcomes. [ABSTRACT FROM AUTHOR]

Published
2024
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16. iTRAQ Proteomics Identified the Potential Biomarkers of Coronary Artery Lesion in Kawasaki Disease and In Vitro Studies Demonstrated That S100A4 Treatment Made HCAECs More Susceptible to Neutrophil Infiltration

Author

Weng, Ken-Pen, primary, Chien, Kuang-Jen, additional, Huang, Shih-Hui, additional, Huang, Lien-Hung, additional, Lin, Pei-Hsien, additional, Lin, Yuyu, additional, Chang, Wei-Hsiang, additional, Chen, Chun-Yu, additional, and Li, Sung-Chou, additional

Published
2022
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17. Exploring the Regulatory Role of XIST-microRNAs/mRNA Network in Circulating CD4 + T Cells of Hepatocellular Carcinoma Patients.

Author

Huang, Lien-Hung, Rau, Cheng-Shyuan, Liu, Yueh-Wei, Wu, Chia-Jung, Chien, Peng-Chen, Lin, Hui-Ping, Wu, Yi-Chan, Huang, Chun-Ying, Hsieh, Ting-Min, and Hsieh, Ching-Hua

Subjects
T cells, GENE expression, CD4 antigen, NUCLEOTIDE sequencing, POLYMERASE chain reaction
Abstract

Hepatocellular carcinoma (HCC) is one of the most common cancers and the main cause of cancer-related death globally. Immune dysregulation of CD4+ T cells has been identified to play a role in the development of HCC. Nevertheless, the underlying molecular pathways of CD4+ T cells in HCC are not completely known. Thus, a better understanding of the dysregulation of the lncRNA-miRNA/mRNA network may yield novel insights into the etiology or progression of HCC. In this study, circulating CD4+ T cells were isolated from the whole blood of 10 healthy controls and 10 HCC patients for the next-generation sequencing of the expression of lncRNAs, miRNAs, and mRNAs. Our data showed that there were different expressions of 34 transcripts (2 lncRNAs, XISTs, and MIR222HGs; 29 mRNAs; and 3 other types of RNA) and 13 miRNAs in the circulating CD4+ T cells of HCC patients. The expression of lncRNA-XIST-related miRNAs and their target mRNAs was confirmed using real-time quantitative polymerase chain reaction (qPCR) on samples from 100 healthy controls and 60 HCC patients. The lncRNA–miRNA/mRNA regulation network was created using interaction data generated from ENCORI and revealed there are positive correlations in the infiltration of total CD4+ T cells, particularly resting memory CD4+ T cells, and negative correlations in the infiltration of Th1 CD4+ T cells. [ABSTRACT FROM AUTHOR]

Published
2023
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18. Serum proteins may facilitate the identification of Kawasaki disease and promote in vitro neutrophil infiltration

Author

Yuyu Lin, Huang Lien-Hung, Kuo-Wang Tsai, Sung-Chou Li, Ken-Pen Weng, Kuang-Jen Chien, Pei-Hsien Lin, and Chi-Ying Tu

Subjects
Male, Immunology, lcsh:Medicine, Diseases, S100A12 Protein, Pathogenesis, Mucocutaneous Lymph Node Syndrome, S100A9, Article, S100A8, Medical research, Rheumatology, Medicine, Humans, lcsh:Science, Defensin, chemistry.chemical_classification, Multidisciplinary, biology, Molecular medicine, Cell adhesion molecule, business.industry, lcsh:R, Infant, Blood Proteins, DNA Methylation, Blood proteins, chemistry, Neutrophil Infiltration, biology.protein, Female, lcsh:Q, Antibody, Glycoprotein, business, Biomarkers
Abstract

Kawasaki disease (KD) usually affects the children younger than 5 years of age and subsequently causes coronary artery lesions (CALs) without timely identification and treatment. Developing a robust and fast prediction method may facilitate the timely diagnosis of KD, significantly reducing the risk of CALs in KD patients. The levels of inflammatory serum proteins dramatically vary during the onsets of many immune diseases, including in KD. However, our understanding of their pathogenic roles in KD is behind satisfaction. The purpose of this study was to evaluate candidate diagnostic serum proteins and the potential mechanism in KD using iTRAQ gel-free proteomics. We enrolled subjects and conducted iTRAQ gel-free proteomics to globally screen serum proteins followed by specific validation with ELISA. Further in vitro leukocyte trans-endothelial model was also applied to investigate the pathogenesis roles of inflammatory serum proteins. We identified six KD protein biomarkers, including Protein S100-A8 (S100A8), Protein S100-A9 (S100A9), Protein S100-A12 (S100A12), Peroxiredoxin-2 (PRDX2), Neutrophil defensin 1 (DEFA1) and Alpha-1-acid glycoprotein 1 (ORM1). They enabled us to develop a high-performance KD prediction model with an auROC value of 0.94, facilitating the timely identification of KD. Further assays concluded that recombinant S100A12 protein treatment activated neutrophil surface adhesion molecules responsible for adhesion to endothelial cells. Therefore, S100A12 promoted both freshly clinically isolated neutrophils and neutrophil-like cells to infiltrate through the endothelial layer in vitro. Finally, the antibody against S100A12 may attenuate the infiltration promoted by S100A12. Our result demonstrated that evaluating S100A8, S100A9, S100A12, PRDX2, DEFA1 and ORM1 levels may be a good diagnostic tool of KD. Further in vitro study implied that S100A12 could be a potential therapeutic target for KD.

Published
2020
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19. Recovery of Dysregulated Genes in Cancer-Related Lower Limb Lymphedema After Supermicrosurgical Lymphaticovenous Anastomosis – A Prospective Longitudinal Cohort Study

Author

Yang,Johnson Chia-Shen, Huang,Lien-Hung, Wu,Shao-Chun, Wu,Yi-Chan, Wu,Chia-Jung, Lin,Chia-Wei, Tsai,Pei-Yu, Chien,Peng-Chen, Hsieh,Ching-Hua, Yang,Johnson Chia-Shen, Huang,Lien-Hung, Wu,Shao-Chun, Wu,Yi-Chan, Wu,Chia-Jung, Lin,Chia-Wei, Tsai,Pei-Yu, Chien,Peng-Chen, and Hsieh,Ching-Hua

Abstract

Johnson Chia-Shen Yang,1,2 Lien-Hung Huang,1,2 Shao-Chun Wu,3,4 Yi-Chan Wu,1,4 Chia-Jung Wu,1,4 Chia-Wei Lin,1,4 Pei-Yu Tsai,1,4 Peng-Chen Chien,1,4 Ching-Hua Hsieh1,2 1Division of Plastic and Reconstructive Surgery, Department of Surgery, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan; 2Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; 3Department of Anesthesiology, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan; 4Chang Gung University College of Medicine, Taoyuan, TaiwanCorrespondence: Ching-Hua HsiehGraduate Institute of Clinical Medical Sciences, Chang Gung University College of Medicine, 123 Dapi Road, Niaosong District, Kaohsiung City, 833, Taiwan, Tel +886-7-7317123, ext.8002, Fax +886-7-7354309, Email prs581126@gmail.comPurpose: This study aims at profiling the expression of dysregulated genes in circulating monocytes of patients with cancer-related lower limb lymphedema before and after treatment with supermicrosurgical lymphaticovenous anastomosis (LVA).Materials and Methods: This prospective longitudinal cohort study enrolled 51 women with post-treatment gynecological cancer, including those with unilateral lymphedema (study group, n = 25) and those without (control group, n = 26). Venous blood samples obtained from the study group before and after LVA and those from the controls were sent for next-generation sequencing, which was validated by real-time PCR. Dysregulated gene expression in the study group, relative to expression in the controls, was recorded before LVA. After one month, postoperative changes in the expression of the identified genes were evaluated. Protein-protein interaction (PPI) was used to investigate dysregulated genes whose expression returned to baseline levels after LVA.Results: Of the 148 preoperative dysregulated genes, which comprised 108 up- and 40 down-regulated genes, 78 genes, consisting of 69 up- and 9 down-regulated genes, showed post-LVA re

Published
2022

24. Increased Angiogenesis by Exosomes Secreted by Adipose-Derived Stem Cells upon Lipopolysaccharide Stimulation

Author

Wu, Shao-Chun, primary, Kuo, Pao-Jen, additional, Rau, Cheng-Shyuan, additional, Huang, Lien-Hung, additional, Lin, Chia-Wei, additional, Wu, Yi-Chan, additional, Wu, Chia-Jung, additional, Tsai, Chia-Wen, additional, Hsieh, Ting-Min, additional, Liu, Hang-Tsung, additional, Huang, Chun-Ying, additional, and Hsieh, Ching-Hua, additional

Published
2021
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27. BST-1 as a serum protein biomarker involved in neutrophil infiltration in schizophrenia.

Author

Wang, Liang-Jen, Huang, Yu-Chi, Lin, Pao-Yen, Lee, Yu, Hung, Chi-Fa, Hsu, Su-Ting, Huang, Lien-Hung, and Li, Sung-Chou

Subjects
NEUTROPHILS, TANDEM mass spectrometry, MACHINE learning, SCHIZOPHRENIA, ENZYME-linked immunosorbent assay, BIOMARKERS
Abstract

Schizophrenia is a serious mental illness. The serum protein biomarkers of schizophrenia were explored using isobaric tags for relative and absolute quantitation (iTRAQ) technology. The underlying function of the identified protein biomarker was also investigated. We first collected serum samples from 12 schizophrenia patients and 12 healthy control (HC) subjects, followed by global screening with iTRAQ and tandem mass spectrometry (LC-MS/MS). In total, 691 serum proteins were detected and eight proteins, including ZYX, OSCAR, TPM4, SDPR, BST1, ARGHDB, ITIH5 and SH3BGRL3, were selected for further specific validation with enzyme-linked immunosorbent assay (ELISA) on the serum samples from 52 schizophrenia patients and 50 HC subjects. Schizophrenia patients had significantly lower serum level of BST1 and higher ITIH5 level than the HC subjects did. Using the levels of BST1, ITIH5 and OSCAR combined with machine learning algorithm, we developed a prediction model of schizophrenia with an auROC value 0.78. Moreover, in vitro cell assay confirmed that BST1 significantly repressed neutrophil infiltration through endothelial layer, highlighted the anti-inflammation nature of BST1. Four novel protein markers (BST1, ITIH5, SDPR, and OSCAR) of schizophrenia were identified, and BST-1 could serve as a serum protein biomarker involved in neutrophil infiltration in schizophrenia. [ABSTRACT FROM AUTHOR]

Published
2022
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32. MiR‐182‐5p enhances in vitro neutrophil infiltration in Kawasaki disease

Author

Chao-Yu Pan, Huang Lien-Hung, Pei-Hsien Lin, Kuo-Wang Tsai, Ken-Pen Weng, Kuang-Jen Chien, Yuyu Lin, and Sung-Chou Li

Subjects
Genetic Markers, Male, 0301 basic medicine, miR‐182‐5p, lcsh:QH426-470, Microarray, Neutrophils, HL-60 Cells, Mucocutaneous Lymph Node Syndrome, neutrophil infiltration, 030105 genetics & heredity, coronary artery lesion, Machine Learning, 03 medical and health sciences, Genetics, medicine, Humans, Gene Regulatory Networks, Child, Molecular Biology, Genetics (clinical), Oligonucleotide Array Sequence Analysis, Migration Assay, Kawasaki disease, Chemistry, Coronary Stenosis, Transendothelial and Transepithelial Migration, Original Articles, Transfection, medicine.disease, Molecular biology, In vitro, Up-Regulation, MicroRNAs, lcsh:Genetics, 030104 developmental biology, Child, Preschool, Biomarker (medicine), Original Article, Female, leukocyte transendothelial migration, Infiltration (medical), Leukocyte Transendothelial Migration Pathway
Abstract

Background Kawasaki disease (KD) patients could develop coronary artery lesion (CAL) which threatens children's life. A previous study identified KD biomarker miRNAs that could discriminate KD patients from febrile non‐KD patients. We wonder whether these KD prediction biomarkers could be further applied to predict CAL formation in KD patients. Methods To examine this hypothesis, we conducted a meta‐analysis, miRNA mimic transfection, in vitro cell model and microarray assays. Results We first showed that miR‐182‐5p and miR‐183‐5p kept higher levels in the KD patients with CAL than those without CAL (p, We showed that miR‐182‐5p could be a biomarker for CAL formation in KD. Its pathogenesis mechanism in CAL formation was also investigated.

Published
2019
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