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322 results on '"Hubel, Allison"'

1. Cryopreservation of mesenchymal stem/stromal cells using a DMSO-free solution is comparable to DMSO-containing cryoprotectants: results of an international multicenter PACT/BEST collaborative study

Author

Mamo, Theodros, Cox, Cheryl A., Demorest, Connor, Fontaine, Magali J., Hubel, Allison, Kelley, Linda, Khan, Aisha, Marks, Denese C., Pati, Shibani, Reems, Jo-Anna, Spohn, Gabriele, Schäfer, Richard, Shi, Rongye, Shao, Lipei, Stroncek, David, and McKenna, David H.

Published
2024
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22. Long‑term storage of gametes and gonadal tissues at room temperatures: the end of the ice age?

Author

Comizzoli, Pierre, Loi, Pasqualino, Patrizio, Pasquale, and Hubel, Allison

Abstract

Long-term preservation of viable spermatozoa, eggs, embryos, and gonadal tissues of good quality is essential in human reproductive medicine and for the population management of livestock, laboratory, and wild species. Instead of using freezing temperatures, encouraging findings indicate that structures and functions of gametes or gonadal tissues can be suspended in trehalose glass after dehydration and then preserved at supra-zero temperatures. As a new era in fertility preservation and biobanking is about to start, the advantages, needs, and implications of germplasm storage at room temperatures must be carefully examined. Although very promising, the development of alternate biobanking strategies does not necessarily mean that the end of the "ice age" (cryopreservation) is near.

Published
2021
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31. Frontiers in biotransport: water transport and hydration

Author

Aksan, Alptekin, Hubel, Allison, and Bischof, John C.

Subjects
Biological transport -- Analysis, Water science -- Research, Engineering and manufacturing industries, Science and technology
Abstract

Biotransport, by its nature, is concerned with the motions of molecules in biological systems while water remains as the most important and the most commonly studied molecule across all disciplines. In this review, we focus on biopreservation and thermal therapies from the perspective of water, exploring how its molecular motions, properties, kinetic, and thermodynamic transitions govern biotransport phenomena and enable preservation or controlled destruction of biological systems. [DOI: 10.1115/1.3173281]

Published
2009

39. Biomechanical and optical characteristics of a corneal stromal equivalent

Author

Orwin, Elizabeth J., Borene, Melinda L., and Hubel, Allison

Subjects
Biomechanics -- Research, Cornea -- Research, Engineering and manufacturing industries, Science and technology
Abstract

Cell matrix interactions are important in understanding the healing characteristics of the cornea after refractive surgery or transplantation. The purpose of this study was to characterize in more detail the evolution of biomechanical and optical properties of a stromal equivalent (stromal fibroblasts cultured in a collagen matrix). Human corneal stromal fibroblasts were cultured in a collagen matrix. Compaction and modulus were determined for the stromal equivalent as a function of time in culture and matrix composition. The corneal stromal fibroblasts were stained for [varies]-smooth muscle actin expression as an indicator of myofibroblast phenotype. The nominal modulus of the collagen matrix was 364 [+ or -] 41 Pa initial and decreased initially with time in culture and then slowly increased to 177 [+ or -] 75 Pa after 21 days. The addition of chondroitin sulfate decreased the contraction of the matrix and enhanced its transparency. Cell phenotype studies showed dynamic changes in the expression of [varies]-smooth muscle actin with time in culture. These results indicate that the contractile behavior of corneal stromal cells can be influenced by both matrix composition and time in culture. Changes in contractile phenotype after completion of the contraction process also indicate that significant cellular changes persist beyond the initial matrix-remodeling phase. [DOI: 10.1115/1.1589773]

Published
2003

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