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Your search keyword '"Huehne, Pattana Srifah"' showing total 13 results
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13 results on '"Huehne, Pattana Srifah"'

7. Proteome level changes in the root of Brassica alboglabra induced by alachlor herbicide

Author

Mohamad, Zuraifah Asrah, Chokchaichamnankit, Daranee, Bhinija, Kisana, Paricharttanakul, N Monique, Svasti, Jisnuson, Huehne, Pattana Srifah, and Srisomsap, Chantragan

Abstract

Chinese kale (Brassica alboglabra) is a famous and extensively grown vegetable in Southeast Asia. Despite its nutritional values, pesticides are heavily applied to it. In this study, changes in protein expression due to alachlor treatment on B. alboglabra were investigated by using the 2-dimensional PAGE. Differential protein expressions were determined by using Image Master Software with volume (%) ≥2 fold as significant. Ten spots of interest have been identified by LC/MS/MS showing significant increases in differential protein expression between B. alboglabra roots treated with alachlor as compared to the untreated group which include HSC-cognate binding proteins, adenosylmethionine synthetase and beta-tubulin involved in defence mechanism in plants. Little is known about the function of other proteins identified which include knox-like proteins and hypothetical protein. Further investigations on plant proteomics would provide more information on the effects of different types of pesticides.Keywords: Brassica alboglabra, pesticides, proteomics, two-dimensional electrophoresisAfrican Journal of Biotechnology Vol. 12(20), pp. 2840-2849

Published
2016

13. Cloning and sequence of cDNA encoding 1-aminocyclopropane- 1-carboxylate oxidase in Vanda flowers.

Author

Lokkamlue, Noppamart and Huehne, Pattana Srifah

Subjects
*NUCLEOTIDE sequence, *CLONING, *ANTISENSE DNA, *AMINOCYCLOPROPANECARBOXYLATE synthase, *VANDA, *OXIDASES
Abstract

The 1-aminocyclopropane-1-carboxylate oxidase (ACO) gene in the final step of ethylene biosynthesis was isolated from ethylene-sensitive Vanda Miss Joaquim flowers. This consists of 1,242 base pairs (bp) encoding for 326 amino acid residues. To investigate the specific divergence in orchid ACO sequences, the deduced Vanda ACO was aligned with five other orchid ACOs. The results reveal that the ACO sequences within Doritaenopsis, Phalaenopsis and Vanda show highly conserved and almost 95% identical homology, while the ACOs isolated from Cymbidium, Dendrobium and Cattleya are 87-88% identical to Vanda ACO. In addition, the 2-oxoglutarate- Fe(II)_oxygenase (Oxy) domain of orchid ACOs consists of a higher degree of amino acid conservation than that of the non-haem dioxygenase (DIOX_N) domain. The overall homology regions of Vanda ACO are commonly folded into 12 α-helices and 12 β-sheets similar to the three dimensional template-structure of Petunia ACO. This Vanda ACO cloned gene is highly expressed in flower tissue compared with root and leaf tissues. In particular, there is an abundance of ACO transcript accumulation in the column followed by the lip and the perianth of Vanda Miss Joaquim flowers at the fullyopen stage. [ABSTRACT FROM AUTHOR]

Published
2013

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