32 results on '"Huiqin LU"'
Search Results
2. Effects of L-Lys on the Quality Characteristics and Oxidation Degree of Yak Meat under MDA Induced Oxidation
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Huiqin LU, Yiwen MEI, Qilan XIE, Linlin WANG, Zijian CAI, Fei QIN, and Pingwei LENG
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l-lysine ,mda ,yak meat ,quality characteristics ,protein oxidation ,Food processing and manufacture ,TP368-456 - Abstract
In order to study the effects of L-Lys on the quality characteristics and protein oxidation of yak meat induced by MDA. In this study, MDA was used as the simulated oxidation system, and yak meat were used as the research objects. The changes of edible quality, lipid oxidation, protein oxidation and muscle histological characteristics during storage were measured after yak meat treated with different concentrations of L-Lys and MDA. The results showed that 10 mmol/L L-Lys could reduce the increase of pH caused by MDA, alleviate the excessive increase of hardness and chewiness, and significantly inhibit the conversion of oxygenated myoglobin into high iron myoglobin, thereby increasing the L* value and a* value (P
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- 2023
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3. Effects of Edible Fungi on Pork Intestine Quality and Volatile Flavor Compounds during Cold Storage
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Pingwei LENG, Yiwen MEI, Huiqin LU, Fei QIN, Yu ZHANG, Lina WANG, and Linlin WANG
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edible fungi ,pork sausage ,quality characteristics ,volatile flavor compounds ,headspace solid phase microextraction ,gc-ms ,Food processing and manufacture ,TP368-456 - Abstract
To investigate the effects of adding natural mushroom powder on pork sausage quality and volatile flavor compounds, in this study, 7 kinds of edible mushroom powder (Shiitake mushroom, Pleurotus eryngii, Pleurotus ostreatus, Agaricus blazei, Hericium erinaceus, and Chicken oil fungus) were added into the pork sausages as the research objects and determined the quality change of pork sausages after storage at 4 ℃ for 1, 3, 5, 7 and 9 days, and the volatile flavor substances of pork sausages were determined and analyzed by GC-MS. The results showed that there were significant differences in the edible quality of sausage added with edible fungi (P
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- 2023
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4. Effect of Arginine Combined with Chitosan on Quality of Postmortem Yak Meat during Maturing
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Yiwen MEI, Jie LI, Rongsheng DU, Linlin WANG, Huiqin LU, Fei QIN, and Pingwei LENG
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yak meat ,arginine ,chitosan ,postmortem ageing ,quality ,oxidation ,Food processing and manufacture ,TP368-456 - Abstract
In order to clarify the synergistic effect of different concentrations of arginine and different concentrations of chitosan on the quality of yak meat during postmortem aging and its potential mechanism, in this study, different concentrations of arginine and different concentrations of chitosan were injected into yak meat to determine their effects on the quality of yak meat during postmortem aging. The results showed that the combination of 0.4%~0.8% arginine and 1.0% chitosan could significantly increase the pH value, reduce the L*, b* values, metmyoglobin and cooking loss of yak meat during postmortem aging, and increase a* value and oxymyoglobin (P0.05). Histological observation showed that the compound treatment was more conducive to the integrity and fullness of muscle cells, and reduced the water channel. In summary, the combination of different concentrations of arginine and different concentrations of chitosan improved the quality of postmortem yak meat during aging to a certain extent, improved its meat color stability and reduced cooking loss, promoted tenderization, and made muscle cells more complete and fuller, but it promoted fat oxidation and protein oxidation as a whole. The specific reasons and related mechanisms need to be further explored.
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- 2023
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5. Positive ground state solutions for a class of fractional coupled Choquard systems
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Kexin Ouyang, Yu Wei, and Huiqin Lu
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fractional choquard systems ,ground state solution ,asymptotic behaviour ,Mathematics ,QA1-939 - Abstract
In this paper, we combine the critical point theory and variational method to investigate the following a class of coupled fractional systems of Choquard type $ \begin{equation*} \left\{ \begin{array}{l} (-\Delta)^{s}u+\lambda_{1}u& = (I_{\alpha}*|u|^{p})|u|^{p-2}u+\beta v \quad &&\text{in}\\ \mathbb{R}^{N}, \ (-\Delta)^{s}v+\lambda_{2}v& = (I_{\alpha}*|v|^{p})|v|^{p-2}v+\beta u \quad &&\text{in}\ \mathbb{R}^{N}, \end{array} \right. \end{equation*} $ with $ s\in(0, 1), \ N\geq 3, \ \alpha\in(0, N), \ p > 1 $, $ \lambda_{i} > 0 $ are constants for $ i = 1, \ 2 $, $ \beta > 0 $ is a parameter, and $ I_{\alpha}(x) $ is the Riesz Potential. We prove the existence and asymptotic behaviour of positive ground state solutions of the systems by using constrained minimization method and Hardy-Littlewood-Sobolev inequality. Moreover, nonexistence of nontrivial solutions is also obtained.
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- 2023
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6. Normalized ground states for fractional Kirchhoff equations with critical or supercritical nonlinearity
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Huanhuan Wang, Kexin Ouyang, and Huiqin Lu
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normalized solution ,fractional kirchhoff equation ,pohozaev manifold ,moser iteration method ,supercritical growth ,Mathematics ,QA1-939 - Abstract
The aim of this paper is to study the existence of ground states for a class of fractional Kirchhoff type equations with critical or supercritical nonlinearity $ (a+b\int_{\mathbb{R}^{3}}|(-\bigtriangleup)^{\frac{s}{2}}u|^{2}dx)(-\bigtriangleup)^{s}u = \lambda u +|u|^{q-2 }u+\mu|u|^{p-2}u, \ x\in\mathbb{R}^{3}, $ with prescribed $ L^{2} $-norm mass $ \int_{\mathbb{R}^{3}}u^{2}dx = c^{2} $ where $ s\in(\frac{3}{4}, \ 1), \ a, b, c > 0, \ \frac{6+8s}{3} < q < 2_{s}^{\ast}, \ p\geq 2^{\ast}_{s}\ (2^{\ast}_{s} = \frac{6}{3-2s}), \ \mu > 0 $ and $ \lambda\in \mathbb{R} $ as a Langrange multiplier. By combining an appropriate truncation argument with Moser iteration method, we prove that the existence of normalized solutions for the above equation when the parameter $ \mu $ is sufficiently small.
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- 2022
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7. Existence of Positive Ground State Solutions for Fractional Choquard Systems in Subcritical and Critical Cases
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Huiqin Lu and Kexin Ouyang
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fractional Choquard systems ,ground state solution ,Nehari method ,Pohožaev identity ,Mathematics ,QA1-939 - Abstract
We investigate a class of fractional linearly coupled Choquard systems. For the subcritical case and all critical cases, we prove the existence, nonexistence and symmetry of positive ground state solutions of systems, by using the Nehari manifold method, the Pohožaev identity and the Schwartz symmetrization rearrangements. In particular, we overcome the lack of compactness of the critical nonlinearities by using the behaviour of sufficiently small Nehari energy levels.
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- 2023
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8. Ground state sign-changing solutions for fractional Laplacian equations with critical nonlinearity
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Mengyu Wang, Xinmin Qu, and Huiqin Lu
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fractional laplacian equation ,sign-changing solution ,ekeland's variational principle ,brouwer's degree theory ,variational methods ,Mathematics ,QA1-939 - Abstract
In this paper, we investigate the existence of the least energy sign-changing solutions for nonlinear elliptic equations driven by nonlocal integro-differential operators with critical nonlinearity. By using constrained minimization method and topological degree theory, we obtain a least energy sign-changing solution for them under much weaker conditions. As a particular case, we drive an existence theorem of sign-changing solutions for the fractional Laplacian equations with critical growth.
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- 2021
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9. Existence and Multiplicity of Positive Solutions for Schrödinger-Kirchhoff-Poisson System with Singularity
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Mengjun Mu and Huiqin Lu
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Mathematics ,QA1-939 - Abstract
We study a singular Schrödinger-Kirchhoff-Poisson system by the variational methods and the Nehari manifold and we prove the existence, uniqueness, and multiplicity of positive solutions of the problem under different conditions.
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- 2017
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10. Nodal Solutions for Some Second-Order Semipositone Integral Boundary Value Problems
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Huiqin Lu, Yang Wang, and Yansheng Liu
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Mathematics ,QA1-939 - Abstract
Using bifurcation techniques, we first prove a global bifurcation theorem for nonlinear second-order semipositone integral boundary value problems. Then the existence and multiplicity of nodal solutions of the above problems are obtained. Finally, an example is worked out to illustrate our main results.
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- 2014
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11. Multiple Positive Solutions for Singular Semipositone Periodic Boundary Value Problems with Derivative Dependence
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Huiqin Lu
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Mathematics ,QA1-939 - Abstract
By constructing a special cone in C1[0,2π] and the fixed point theorem, this paper investigates second-order singular semipositone periodic boundary value problems with dependence on the first-order derivative and obtains the existence of multiple positive solutions. Further, an example is given to demonstrate the applications of our main results.
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- 2012
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12. Eigenvalue Problem and Unbounded Connected Branch of Positive Solutions to a Class of Singular Elastic Beam Equations
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Huiqin Lu
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Analysis ,QA299.6-433 - Published
- 2011
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13. Positive solution for a class of nonlocal elliptic equations.
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Huiqin Lu and Xingqiu Zhang
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- 2019
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14. Existence of Positive Ground State Solutions for Fractional Choquard Systems in Subcritical and Critical Cases
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Ouyang, Huiqin Lu and Kexin
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fractional Choquard systems ,ground state solution ,Nehari method ,Pohožaev identity - Abstract
We investigate a class of fractional linearly coupled Choquard systems. For the subcritical case and all critical cases, we prove the existence, nonexistence and symmetry of positive ground state solutions of systems, by using the Nehari manifold method, the Pohožaev identity and the Schwartz symmetrization rearrangements. In particular, we overcome the lack of compactness of the critical nonlinearities by using the behaviour of sufficiently small Nehari energy levels.
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- 2023
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15. Ground state sign-changing solutions for fractional Laplacian equations with critical nonlinearity
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Huiqin Lu, Xinmin Qu, and Mengyu Wang
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ekeland's variational principle ,sign-changing solution ,lcsh:Mathematics ,General Mathematics ,Mathematical analysis ,variational methods ,Existence theorem ,Topological degree theory ,lcsh:QA1-939 ,Ekeland's variational principle ,fractional laplacian equation ,Nonlinear system ,brouwer's degree theory ,Minification ,Fractional Laplacian ,Ground state ,Energy (signal processing) ,Mathematics - Abstract
In this paper, we investigate the existence of the least energy sign-changing solutions for nonlinear elliptic equations driven by nonlocal integro-differential operators with critical nonlinearity. By using constrained minimization method and topological degree theory, we obtain a least energy sign-changing solution for them under much weaker conditions. As a particular case, we drive an existence theorem of sign-changing solutions for the fractional Laplacian equations with critical growth.
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- 2021
16. Multiplicity Results for Second Order Impulsive Differential Equations via Variational Methods
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Dan Lu, Huanhuan Wang, and Huiqin Lu
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symbols.namesake ,Class (set theory) ,Variational method ,Critical point (set theory) ,Differential equation ,Product (mathematics) ,Multiplicity results ,Dirichlet boundary condition ,symbols ,Order (group theory) ,Applied mathematics ,Mathematics - Abstract
In this paper we investigate a class of impulsive differential equations with Dirichlet boundary conditions. Firstly, we define new inner product of and prove that the norm which is deduced by the inner product is equivalent to the usual norm. Secondly, we construct the lower and upper solutions of (1.1). Thirdly, we obtain the existence of a positive solution, a negative solution and a sign-changing solution by using critical point theory and variational methods. Finally, an example is presented to illustrate the application of our main result.
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- 2021
17. Prosthetic control system based on motor imagery
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Xiaoyan Shen, Yan Wang, Lei Ma, Xuemei Wang, and Huiqin Lu
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Artificial neural network ,Computer science ,business.industry ,Feature vector ,Biomedical Engineering ,Bioengineering ,Pattern recognition ,Electroencephalography ,General Medicine ,Hand ,Backpropagation ,Computer Science Applications ,Human-Computer Interaction ,Sample entropy ,Motor imagery ,Wavelet ,Feature (computer vision) ,Brain-Computer Interfaces ,Activities of Daily Living ,Imagination ,Humans ,Artificial intelligence ,business ,Algorithms ,Brain–computer interface - Abstract
A brain-computer interface (BCI) can be used for function replacement through the control of devices, such as prostheses, by identifying the subject's intent from brain activity. We process electroencephalography (EEG) signals related to motor imagery to improve the accuracy of intent classification. The original signals are decomposed into three layers based on db4 wavelet basis. The wavelet soft threshold denoising method is used to improve the signal-to-noise ratio. The sample entropy algorithm is used to extract the features of the signal after noise reduction and reconstruction. Combined with event-related synchronisation/desynchronisation (ERS/ERD) phenomenon, the sample entropy in the motor imagery time periods of C3, C4 and Cz is selected as the feature value. Feature vectors are then used as the input of three classifiers. From the evaluated classifiers, the backpropagation (BP) neural network provides the best EEG signal classification (93% accuracy). BP neural network is thus selected as the final classifier and used to design a prosthetic control system based on motor imagery. The classification results are wirelessly transmitted to control a prosthesis successfully via commands of hand opening, fist clenching, and external wrist rotation. Such functionality may allow amputees to complete simple activities of daily living. Thus, this study is valuable for subsequent developments in rehabilitation.
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- 2021
18. Sign-changing and constant-sign solutions for elliptic problems involving nonlocal integro-differential operators
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Huiqin Lu, Xinmin Qu, and Jianing Wang
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Nonlinear system ,Lemma (mathematics) ,Partial differential equation ,Numerical analysis ,Mathematical analysis ,Mathematics::Analysis of PDEs ,Lipschitz continuity ,Constant (mathematics) ,Differential operator ,Mathematics ,Sign (mathematics) - Abstract
This work is devoted to study the existence of sign-changing and constant-sign solutions for nonlinear elliptic problems driven by nonlocal integro-differential operators with subcritical nonlinearity which is not locally Lipschitz continuous and there is no (AR) condition and no any monotony properties. By using some variants of the Mountain Pass Lemma, we show the existence of a positive solution, a negative solution and a sign-changing solution.
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- 2020
19. P53/DRAM/ autophagy - a new target for improving the therapeutic effect of anti-VEGF on intraocular neovascularization
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Yi Wang, Yao Yang, Rong Li, Binghui Wu, Huiqin Lu, Jing Cheng, Zhe Liu, and Junhui Du
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Backgroud Recurrence of intraocular neovascularization is a major clinical problem. Anti-VEGF drugs are the main treatment for intraocular neovascularization currently. However, anti-VEGF drugs can activate endothelial autophagy and weaken the therapeutic effect. This study aims to elucidate the effect and mechanism of anti-VEGF drugs on autophagy of vascular endothelial cells. Methods RF/6A cells were randomly divided into five groups: The control group, hypoxia group (1% O2、5% CO2、94% N2), anti-VEGF group(group1:Ranibizumab 100ug/ml; group2: Aflibercept, 400ug/ml; group3: Conbercept, 100ug/ml) and autophagy inhibition group(3-MA or CQ) which was corresponding to anti-VEGF group. Autophagy-related proteins were examined by Western blot. RFP-GFP-LC3 was used to detect autophagy and autophagic flow. CCK-8 assay was used to detect cell proliferation. Flow cytometry and Tunel was used to detect cell apoptosis. Cell migration and tube formation were assessed by wound assay and matrix method, respectively. Results Ranibizumab and Conbercept can triger autophagy in hypoxia condition in RF/6A cells, while Aflibercept can inhibit autophagy. Conbercept combined with autophagy inhibitor (3-MA or CQ) could inhibit cell migration and tube formation of RF/6A cells more effectively in hypoxia condition. For mechanism, p53 and DRAM proteins paly an important role in Conbercept induced autophagy. Inhibition of P53 can suppressed the autophagy induced by Conbercept. Conclusion Ranibizumab and Conbercept can triger the autophagy of vascular endothelial cells while Aflibercept can inhibit it. The combination of ranibizumab/ Concept and autophagy inhibitor can significantly inhibit the formation of angiogenesis in vitro. The mechanism of autophagy activation is related to the activation of p53 / DRAM pathway.
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- 2020
20. Different effects of anti-VEGF drugs (Ranibizumab, Aflibercept, Conbercept) on autophagy and its effect on neovascularization in RF/6A cells
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Junhui Du, Zhe Liu, Binghui Wu, Huiqin Lu, Rong Li, Yi Wang, Yang Yao, and Jing Cheng
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0301 basic medicine ,Angiogenesis ,Recombinant Fusion Proteins ,Neovascularization, Physiologic ,Angiogenesis Inhibitors ,Apoptosis ,030204 cardiovascular system & hematology ,Biochemistry ,Cell Line ,Neovascularization ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Ranibizumab ,Autophagy ,medicine ,Animals ,Cell Proliferation ,Aflibercept ,Tube formation ,Vascular Endothelial Growth Factors ,Cell growth ,business.industry ,Endothelial Cells ,Cell Biology ,Macaca mulatta ,Choroidal Neovascularization ,Receptors, Vascular Endothelial Growth Factor ,030104 developmental biology ,Choroidal neovascularization ,Cancer research ,Tumor Suppressor Protein p53 ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Signal Transduction ,medicine.drug - Abstract
Choroidal neovascularization (CNV) is the main pathological change of wet age-related macular degeneration. Anti-VEGF drugs are the most commonly used treatment for CNV. The biggest drawback of anti-VEGF drugs is the recurrence of CNV, which requires repeated therapy several times. Autophagy activation may be involved in reducing the therapeutic effect of anti-VEGF drugs. So, this study aims to elucidate the effect and mechanism of anti-VEGF drugs on endothelial autophagy and neovascularization in vitro.RF/6A cells were randomly divided into five groups: The control group, hypoxia group (1% OThe protein levels of Beclin-1 and LC3-2/1 in Ranibizumab and Conbercept groups were significantly higher than in the hypoxia group(P 0.05). While the expression of P62 was decreased (P 0.05). The autophagic flux was showed the same results. However, Aflibercept showed the opposite effect on autophagy. Compared with the Conbercept group, autophagy inhibitor 3-MA or CQ can further inhibit cell proliferation and promotes cell apoptosis (P 0.05). Conbercept significantly inhibited cell migration compared with the hypoxia group (633.08 ± 72.52 vs. 546.33 ± 24.61), while the autophagy inhibitor group (3-MA or CQ) had a more obvious inhibition effect (309.75 ± 86.36 and 263.33 ± 68.67) (P 0.05). For tube formation, the number of tube formation was decreased significantly in the Conbercept group (32.00 ± 2.00) compared to the hypoxia group (39.00 ± 1.53) and even further reduced in 3-MA or CQ group (24.00 ± 3.61, 20.00 ± 2.65). The length of master segments in the hypoxia group was 15,668.00 ± 894.11. It was decreased in Conbercept (13,885.34 ± 730.03). In 3-MA or CQ group, the length of master segments dropped further (11,997.00 ± 433.66, 10,617.67 ± 543.21). Compare with the hypoxia group, the expression P53 and DRAM were increased in the Conbercept group (P 0.05). Autophagy-related proteins LC-3, Beclin-1, and DRAM were inhibited by P53 inhibitor Pifithrin-α (PFTα) (P 0.05).Ranibizumab and Conbercept can trigger the autophagy of vascular endothelial cells while Aflibercept can inhibit it. The combination of Conbercept and autophagy inhibitor can significantly inhibit the formation of angiogenesis in vitro. The mechanism of autophagy activation is related to the activation of the p53/DRAM pathway.
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- 2021
21. Identification of hepatitis B virus infection and integration and its oncogenic role in gastric cancer
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Mengge Li, Shusheng Wu, Jiayu Niu, Huiqin Luo, Wenju Chen, Lulu Cao, Ying Yan, Hong Tu, and Yifu He
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Medicine (General) ,R5-920 - Published
- 2024
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22. Automated quantification of superficial retinal capillaries and large vessels for diabetic retinopathy on optical coherence tomographic angiography
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Feng Xu, Jianqin Lei, Cheng Chen, Huiqin Lu, Xiayu Xu, Wenxiang Ding, and Peiwei Yang
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medicine.medical_specialty ,General Physics and Astronomy ,Large vessel ,01 natural sciences ,General Biochemistry, Genetics and Molecular Biology ,Microcirculation ,010309 optics ,chemistry.chemical_compound ,Automation ,Ophthalmology ,0103 physical sciences ,medicine ,Image Processing, Computer-Assisted ,Humans ,General Materials Science ,Diabetic Retinopathy ,medicine.diagnostic_test ,business.industry ,010401 analytical chemistry ,General Engineering ,Angiography ,Retinal Vessels ,Retinal ,General Chemistry ,Optical coherence tomography angiography ,Diabetic retinopathy ,Foveal avascular zone ,medicine.disease ,0104 chemical sciences ,Capillaries ,Cross-Sectional Studies ,chemistry ,Case-Control Studies ,business ,Tomography, Optical Coherence ,Retinopathy - Abstract
Optical coherence tomography angiography (OCTA) is a relatively new technique with capillary-level resolution, which has shown great potential for the diagnosis of diabetic retinopathy (DR). A fully automatic algorithm for the quantitative measurement of microcirculatory changes in sight-threatening DR is presented. The foveal avascular zone (FAZ) segmentation was improved with a graph-theoretic method and the large vessels and capillaries were separately identified and analyzed. The method was evaluated in healthy and diabetic eyes with various stages of retinopathy. Results showed that, compared with the healthy group, the diabetic group showed a significantly larger large vessel density, but a significantly smaller capillary density (P < .001). Circularity of FAZ was significantly smaller while nonperfusion area was significantly larger in the diabetic group. The combined variable of all image metrics reached an area under the ROC of 0.853 (95% CI, 0.784-0.923) for mild to moderate nonproliferative DR and 0.950 (95% CI, 0.922-0.979) for proliferative DR. Microvascular and FAZ changes with various DR stages can be accurately delineated using the developed automatic program. Quantitative metrics on OCTA serve as potential biomarkers for the staging of DR.
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- 2019
23. Distinctive Analysis of Macular Superficial Capillaries and Large Vessels Using Optical Coherence Tomographic Angiography in Healthy and Diabetic Eyes
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Yan Suo, Cheng Chen, Enhui Yi, Nizar Saleh Abdelfattah, Xiayu Xu, Jianqin Lei, Huiqin Lu, Wenxiang Ding, and Xiaojuan Fan
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0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Fovea Centralis ,03 medical and health sciences ,chemistry.chemical_compound ,Young Adult ,0302 clinical medicine ,Optical coherence tomography ,Ophthalmology ,Diabetes mellitus ,medicine ,Humans ,Fluorescein Angiography ,Aged ,Aged, 80 and over ,Diabetic Retinopathy ,medicine.diagnostic_test ,business.industry ,Fovea centralis ,Retinal Vessels ,Retinal ,Diabetic retinopathy ,Middle Aged ,Fluorescein angiography ,medicine.disease ,eye diseases ,Healthy Volunteers ,Capillaries ,030104 developmental biology ,medicine.anatomical_structure ,Cross-Sectional Studies ,chemistry ,Angiography ,030221 ophthalmology & optometry ,Female ,business ,Perfusion ,Algorithms ,Tomography, Optical Coherence - Abstract
Purpose To quantify and evaluate macular superficial capillaries and large vessels separately using an optical coherence tomographic angiography (OCTA)-based automatic segmentation algorithm. Methods In this cross-sectional study, all eyes were scanned using an OCTA device with 3 × 3 mm cube centered on the fovea. Retinal large vessels (arterioles/venules) were automatically segmented from superficial vasculature en-face images. All images were normalized, binarized, and skeletonized for quantification. Metrics of retinal capillaries were calculated by subtracting the measurements of large vessels from total vasculature. Perfusion density (PD), vessel length density (VLD), and vessel diameter index (VDI) within Early Treatment Diabetic Retinopathy Study (ETDRS) 3-mm ring were calculated for total superficial vasculature, large vessels (PDlarge, VLDlarge, and VDIlarge) and capillaries (PDcap, VLDcap, and VDIcap), respectively. Results Fifty-nine eyes from 59 healthy participants (mean age, 45 ± 14 years, 36 females) and 118 eyes from 67 patients with diabetes mellitus (mean age, 57 ± 10 years, 28 females) were included. The diabetic cohort included four subgroups (35 eyes without diabetic retinopathy, 30 eyes with mild to moderate nonproliferative diabetic retinopathy [NPDR], 27 eyes with severe NPDR, and 26 eyes with PDR). Linear regression showed that all above metrics were correlated with the disease stage (from healthy state to PDR), and the β value was -0.76, 0.24, -0.78, 0.80, 0.30, 0.77, -0.81, 0.16, and -0.82 for VD, VDlarge, VDcap, VDI, VDIlarge, VDIcap, VLD, VLDlarge, and VLDcap, respectively. Conclusions Retinal capillaries and large vessels responded differently in the context of diabetes. VLD of capillary is a potentially reliable metric in diabetic retinopathy staging.
- Published
- 2018
24. Nodal Solutions for Some Second-Order Semipositone Integral Boundary Value Problems
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Yansheng Liu, Yang Wang, and Huiqin Lu
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Nonlinear system ,Article Subject ,lcsh:Mathematics ,Applied Mathematics ,Mathematical analysis ,Multiplicity (mathematics) ,Boundary value problem ,lcsh:QA1-939 ,Analysis ,Bifurcation ,Mathematics - Abstract
Using bifurcation techniques, we first prove a global bifurcation theorem for nonlinear second-order semipositone integral boundary value problems. Then the existence and multiplicity of nodal solutions of the above problems are obtained. Finally, an example is worked out to illustrate our main results.
- Published
- 2014
25. Serological and Molecular Characterization of Hepatitis B Virus Infection in Gastric Cancer
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Mengge Li, Shusheng Wu, Huiqin Luo, Jiayu Niu, Ying Yan, Yuan Fang, Lihong Ke, Wenju Chen, Huijun Xu, Huimin Li, Xiaoxiu Hu, Lulu Cao, Yaolin Chen, Hong Tu, and Yifu He
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gastric cancer ,hepatitis B virus ,infection ,prognosis ,mutation profiles ,Microbiology ,QR1-502 - Abstract
Hepatitis B virus (HBV) infection has been reported to be associated with gastric cancer (GC). Nonetheless, no study has revealed the role of HBV infection in the survival of patients with GC, and the mutation profiles of HBV-infected patients with GC have never been documented. Here, we performed an updated meta-analysis and found a significantly increased risk of GC in HBV-infected individuals (sOR, 1.29; 95% CI, 1.22-1.37). Furthermore, we observed that in the Anhui area, the rate of serum HBsAg positivity (OR, 1.62; 95% CI, 1.03-2.55) was significantly higher in GC patients than in controls. Moreover, our results showed that HBV-positive patients had significantly worse disease-free survival (HR, 1.98; 95% CI, 1.39-2.82) and overall survival (HR, 1.84; 95% CI, 1.19-2.85) than HBV-negative patients. The results of Cox proportional hazards regression proved that HBV infection was an independent adverse prognostic factor in GC. Furthermore, by performing targeted-NGS, we found unique mutation profiles in HBV-infected GC samples, including five frequently mutated protein-coding genes (KMT2B, KMT2D, SOX1, FGF12, and TUBB2B). Expression and survival analyses of these genes identified three novel candidate genes that may have potential roles in GC development. Gene Ontology enrichment analysis showed that the recurrent mutations in HBV-positive GC samples were related to cell proliferation, cell migration, and transcription. Taking together, our study proved that HBV infection is an independent prognostic factor in GC patients. The unique mutation profiles of HBV-infected patients with GC open a new research direction toward the underling mechanism between HBV infection and GC.
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- 2022
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26. Genome-wide pathways analysis of nickel ion-induced differential genes expression in fibroblasts
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Lifeng Zhao, Xiaoying Lü, Yamin Yang, Zu Hong Lu, and Huiqin Lu
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Biophysics ,Bioengineering ,Biocompatible Materials ,Biology ,Flow cytometry ,Biomaterials ,Biological pathway ,Mice ,Nickel ,Complementary DNA ,Gene expression ,medicine ,Animals ,Humans ,Gene Regulatory Networks ,Gene ,Cell Proliferation ,Oligonucleotide Array Sequence Analysis ,Genome ,medicine.diagnostic_test ,Microarray analysis techniques ,Gene Expression Profiling ,Fibroblasts ,Molecular biology ,Real-time polymerase chain reaction ,Mechanics of Materials ,Ceramics and Composites ,Gene chip analysis ,Signal Transduction - Abstract
To reveal molecular mechanisms of the interaction between Ni2+ and cells, cDNA microarray technology and GenMAPP analysis were utilized to investigate changes of gene expression profile and identify significant biological pathways in mouse fibroblast cells (L-929) treated by 100 microm Ni2+ for 12, 24, 48 and 72 h, respectively. The microarray data was validated by real-time PCR. Methylthiazoltetrazolium (MTT) analysis and flow cytometry experiment were used to assess the cellular response of L-929 cells to Ni2+. It was found that six main biological pathways were affected by Ni2+ with 118 differentially expressed genes involved. Further analysis illuminated that the exposure of cells to Ni2+ may evoke series of cellular responses to hypoxia by regulating hypoxia-inducible gene expression and cause irreversible DNA damage. Cell cycle pathway analysis results showed DNA replication in S phase could be inhibited by Ni2+ which was consistent with the data gained from flow cytometry experiment. Compared to previous researches based on conventional molecular biology experiments, the present work has not only indirectly validated the findings of other groups but also obtained several discoveries related to cell-Ni2+ interaction, such as inhibition of electron transport chain and accumulation of extracellular matrix (ECM) collagens. The routine of the present study not only can analyze gene expression profile but also may provide a more convenient and efficient approach to explain molecular mechanisms of cell-biomaterial interaction.
- Published
- 2009
27. Mechanisms of cytotoxicity of nickel ions based on gene expression profiles
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Xiang Bao, Huiqin Lu, Zu Hong Lu, Xiaoying Lü, Yan Huang, and Yinghua Qu
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Microarray ,Cell growth ,Gene Expression Profiling ,Biophysics ,Gene Expression ,Bioengineering ,Apoptosis ,Cell Differentiation ,Biology ,Molecular biology ,Cell Line ,Biomaterials ,Mice ,Mechanics of Materials ,Nickel ,Complementary DNA ,Gene expression ,Ceramics and Composites ,Cytotoxic T cell ,Animals ,Cytotoxicity ,Gene ,Cell Proliferation ,Oligonucleotide Array Sequence Analysis - Abstract
This study investigated cytotoxic effects of Ni(II) to mouse fibroblast cells (L-929) on the level of gene expression profiles with cDNA microarray. The gene expression profiles of L-929 were detected after the cells were cultured in the medium with 200 μ m Ni(II) for 24, 48 and 72 h, respectively, and the cytotoxicity of Ni(II) was evaluated with methylthiazoltetrazolium (MTT) assay. 20 up-regulated genes and 19 down-regulated genes were differentially expressed in all three-culture periods. Gene ontology analysis showed that the L-929 cells which responded to Ni(II) covered a broad range of functional gene groups including cellular biological process, molecular function, and cellular component. Ni(II) has extensive effects on cells by inhibiting cell proliferation and differentiation through inducing cell apoptosis, affecting cell development and influencing cholesterol metabolism.
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- 2008
28. Prognostic Significance of Platelet (PLT) and Platelet to Mean Platelet Volume (PLT/MPV) Ratio During Apatinib Second-Line or Late-Line Treatment in Advanced Esophageal Squamous Cell Carcinoma Patients
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Rixin Su MMed, Jingya Zhu MMed, Shusheng Wu MMed, Huiqin Luo MMed, and Yifu He MD
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: Apatinib has a certain efficacy for advanced esophageal squamous cell carcinoma (ESCC). This study aimed to investigate the prognostic significance of platelet (PLT) and platelet to mean platelet volume (PLT/MPV) ratio for advanced ESCC patients with apatinib second-line or late-line treatment. Methods: A retrospective study included 80 patients with advanced ESCC who received Apatinib ≥ 2 lines targeted therapy. We collected baseline clinical characteristics and blood parameters from the patients. Kaplan–Meier plots and univariate and multivariate analysis were used to find the factors related to progression-free survival (PFS). Results: The optimal cut-off values of PLT and PLT/MPV ratio were determined by X-tile software. Kaplan–Meier analysis demonstrated that patients in the high PLT group had better PFS than those in the low PLT group (156 d vs 80 d, P
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- 2022
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29. Influence of ion implantation on the reflectance spectrum of HgCdTe
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Runqing Jiang, Xiaoning Hu, Jun Zhao, JianHua Zhao, Huiqin Lu, Xinwen Hu, Wenjuan Zhu, Xiangyang Li, Jiaxiong Fang, and Yueyuan Xia
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Microscope ,Materials science ,business.industry ,Infrared ,education ,law.invention ,Ion ,chemistry.chemical_compound ,Ion implantation ,Optics ,chemistry ,law ,Reflection (physics) ,sense organs ,Mercury cadmium telluride ,business ,Layer (electronics) ,Refractive index - Abstract
An obvious minimum was observed in the reflectance spectrum of ion implanted Hg1-xCdxTe. The anodic oxidation method and beveling technique were used to learn the spectrum changing with the depth. This minimum even can be observed when a layer with thickness thicker than the ion range was removed from the implanted surface. Considering the high electron concentration of the implanted layer, this phenomenon was explained by using the model of reflection of layered media in which the refractive index changes with the depth. By numerical fitting, the depth profile of the carrier density and refractive the index of the ion implanted layer were obtained.© (1998) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
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- 1998
30. The bone alterations in hind limb amputation rats in vivo
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Huiqin Luan, Huiru gu, Zhongjun Mo, Weiyan Ren, Huan Guo, Zhaowei Chu, and Yubo Fan
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Amputation ,Micro-CT ,Microstructure ,Bone loss ,BMD ,Medical technology ,R855-855.5 - Abstract
Osteoporosis, which is characterized by bone loss, increases the risk of fractures and s and seriously affected the amputee’s health. Muscle mass is an important factor affecting bone mineral density (BMD). Muscular atrophy and decreased muscle mass can cause BMD to fall. The bone quantity and quality are used to assess by Microcomputer tomography (micro-CT) with non-destructive. To investigate the performance of micro-CT in evaluating amputation-induced osteopenia in rats, the micro-CT was used to evaluate the change of the microstructure in trabecular bone and cortical bone of amputation. Muscle morphology was analyzed by micro-CT and transmission electron microscopy. Female Sprague–Dawley rats (8 week old) were divided into two groups: control (CON) groups and left hind limb amputation (LHLA). At 35 days, microstructure and volumetric bone mineral density (vBMD) of the tibia and femur were detected through micro-CT. The results showed: (1) the trabecular and cortical vBMD of the proximal tibia and distal femur in the LHLA group were significantly lower than those in the CON group. (2) Compared with the CON group, the trabecular number, bone volume fraction and trabecular thickness of proximal tibia and distal femur in LHLA group decreased significantly, whereas the trabecular separation, structure model index and bone surface/bone volume increased significantly in LHLA group. (3) The whole cross-sectional area (CSA) of whole calf muscles of left limb in LHLA group decreased significantly compared with CON group. In general, hind limb amputation could cause bone loss, changes in the microstructure of trabecular bone and muscle atrophy. Micro-CT is sensitive in evaluating bone microstructure destruction caused by amputation, and can be used for early diagnosis or monitor the development of bone loss in amputation in vivo.
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- 2020
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31. Effects of Local Vibration With Different Intermittent Durations on Skin Blood Flow Responses in Diabetic People
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Weiyan Ren, Fang Pu, Huiqin Luan, Yijie Duan, Honglun Su, Yubo Fan, and Yih-Kuen Jan
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diabetes mellitus ,diabetic foot ulcers ,vibration ,skin blood flow ,microcirculation ,Biotechnology ,TP248.13-248.65 - Abstract
Objective: Poor blood flow supply is an important pathological factor that leads to the development and deterioration of diabetic foot ulcers. This study aims to investigate the acute effects of local vibration with varying intermittent durations on the plantar skin blood flow (SBF) response in diabetic and healthy subjects.Methods: Eleven diabetic patients (7 males, 4 females) and 15 healthy adults (6 males, 9 females) participated in this experiment and accepted three tests. Local continuous vibration (LCV) and two levels of local intermittent vibration (LIV1 and LIV2) were randomly applied to the middle metatarsal head of each subject's right foot in each test. The SBF was measured prior to intervention (Baseline), during Vibration and during the Recovery Stage for each test. The mean SBF in each stage, the change percentages and change rates of SBF in Vibration and Recovery stage among three tests were compared and analyzed for both diabetic and healthy subjects.Results: For diabetic subjects, the SBF was significantly increased in both Vibration and Recovery Stage with local intermittent vibrations (LIV1 and LIV2), but not with LCV. However, there was no significant difference in change percentage and change rate of SBF in diabetic subjects across the three tests. For healthy subjects, all vibration interventions significantly increased the SBF in the Vibration Stage and in the first 1.5 min of the Recovery Stage. Also, the change rate of SBF during the Vibration stage in LIV1 test was significantly greater than that in LIV2 test for healthy subjects. Moreover, change percentage of SBF in Vibration stage of LIV1 test and in some periods of Recovery stages of LIV1 and LIV2 tests for diabetic subjects were lower than for healthy subjects; the absolute change rate of SBF in LIV1 test for diabetic subjects was also lower than for healthy subjects.Conclusion: These findings suggest that both LIV1 and LIV2 may effectively improve SBF in the feet of diabetic people, but LCV may not achieve the same level of vasodilatation. The diabetic subjects were also found to have a lower SBF response to applied vibration than the healthy subjects.
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- 2019
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32. Recent Update on the Pharmacological Effects and Mechanisms of Dihydromyricetin
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Jingyao Zhang, Yun Chen, Huiqin Luo, Linlin Sun, Mengting Xu, Jin Yu, Qigang Zhou, Guoliang Meng, and Shengju Yang
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dihydromyricetin ,cardioprotection ,hepatoprotection ,neuroprotection ,oxidative stress ,apoptosis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
As the most abundant natural flavonoid in rattan tea, dihydromyricetin (DMY) has shown a wide range of pharmacological effects. In addition to the general characteristics of flavonoids, DMY has the effects of cardioprotection, anti-diabetes, hepatoprotection, neuroprotection, anti-tumor, and dermatoprotection. DMY was also applied for the treatment of bacterial infection, osteoporosis, asthma, kidney injury, nephrotoxicity and so on. These effects to some extent enrich the understanding about the role of DMY in disease prevention and therapy. However, to date, we still have no outlined knowledge about the detailed mechanism of DMY, which might be related to anti-oxidation and anti-inflammation. And the detailed mechanisms may be associated with several different molecules involved in cellular apoptosis, oxidative stress, and inflammation, such as AMP-activated protein kinase (AMPK), mitogen-activated protein kinase (MAPK), protein kinase B (Akt), nuclear factor-κB (NF-κB), nuclear factor E2-related factor 2 (Nrf2), ATP-binding cassette transporter A1 (ABCA1), peroxisome proliferator-activated receptor-γ (PPARγ) and so on. Here, we summarized the current pharmacological developments of DMY as well as possible mechanisms, aiming to push the understanding about the protective role of DMY as well as its preclinical assessment of novel application.
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- 2018
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