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Macrophages play an important role in the pathogenesis of systemic lupus erythematosus-associated diffuse alveolar hemorrhage (DAH). The immunomodulation of macrophage responses might be a potential approach for the prevention and treatment of DAH. Erythropoietin (EPO) could regulate macrophage bioactivities by binding to the EPO receptor expressing on macrophages. This study assessed the effects of EPO on DAH protection using an immune-mediated DAH murine model with macrophages as the major contributor. A DAH murine model was established in female C57BL/6 mice by an i.p. injection of pristane. We found that EPO administration alleviates DAH by reducing pulmonary macrophages recruitment and promoting phenotype switch toward M2 macrophages in vivo. EPO drove macrophages to the anti-inflammatory phenotype in the primary murine bone marrow-derived macrophages and macrophages cell line RAW 264.7 with LPS, IFN-γ, and IL-4 in vitro. Moreover, EPO treatment increases the expression of EPOR and decreases the expression of miR-494-3p, resulting in increased phosphorylation of JAK2 and STAT3. In conclusion, EPO can be a potential therapeutic agent in DAH by reducing cell apoptosis and regulating macrophage polarization through the EPOR/JAK2/STAT3 axis. Further studies are also needed to validate the direct target of miR-494-3p in regulating JAK2/STAT3 signaling transduction. [ABSTRACT FROM AUTHOR]

The electromagnetic interference (EMI) caused by alternating current driven LEDs which are widely used as indicator lamps in power strip is presented. The frequency-domain measurement results show that the EMI will violate the MIL-461E/RE102 standard limit line in the electromagnetic compatibility anechoic chamber. The timedomain measurement results show that the breakdown of LEDs suddenly increase the current causing the interference. The colour light of LED is determined by the energy bandgap of the semiconductor, as they may influence the EMI level, red and yellow LEDs are analysed. [ABSTRACT FROM AUTHOR]