1. The Effect of S-Allyl L-Cysteine on Retinal Ischemia: The Contributions of MCP-1 and PKM2 in the Underlying Medicinal Properties
- Author
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Windsor Wen-Jin Chao, Howard Wen-Haur Chao, Hung-Fu Lee, and Hsiao-Ming Chao
- Subjects
S-allyl L-cysteine ,retinal pigment epithelium ,retinal ganglion cell ,pyruvate kinase M2 ,monocyte chemoattractant protein-1 ,hydrogen peroxide ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Retinal ischemia plays a vital role in vision-threatening retinal ischemic disorders, such as diabetic retinopathy, age-related macular degeneration, glaucoma, etc. The aim of this study was to investigate the effects of S-allyl L-cysteine (SAC) and its associated therapeutic mechanism. Oxidative stress was induced by administration of 500 μM H2O2 for 24 h; SAC demonstrated a dose-dependent neuroprotective effect with significant cell viability effects at 100 μM, and it concurrently downregulated angiogenesis factor PKM2 and inflammatory biomarker MCP-1. In a Wistar rat model of high intraocular pressure (HIOP)-induced retinal ischemia and reperfusion (I/R), post-administration of 100 μM SAC counteracted the ischemic-associated reduction of ERG b-wave amplitude and fluorogold-labeled RGC reduction. This study supports that SAC could protect against retinal ischemia through its anti-oxidative, anti-angiogenic, anti-inflammatory, and neuroprotective properties.
- Published
- 2024
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