84 results on '"Huo JH"'
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2. 112-gbit/s PDM-PAM4 transmission over 80-km SMF using digital coherent detection without optical amplifier
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Chao LU, Lau, Apt, Yu, Cy, Zeng, L., Gao, L., Yang, Yf, Tu, Jj, Wang, L., Wang, Yg, Huo, Jh, Zhong, Kp, and Zhou, X.
3. Altering electronic structure of nickel foam supported CoNi-based oxide through Al ions modulation for efficient oxygen evolution reaction.
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Saqib Rabbani M, Chen JH, Duan YX, Cui RC, Du X, Liu ZY, Imran Anwar M, Zafar Z, and Yue XZ
- Abstract
Developing highly active and durable non-precious metal-based electrocatalysts for the oxygen evolution reaction (OER) is crucial in achieving efficient energy conversion. Herein, we reported a CoNiAl
0.5 O/NF nanofilament that exhibits higher OER activity than previously reported IrO2 -based catalysts in alkaline solution. The as-synthesized CoNiAl0.5 O/NF catalyst demonstrates a low overpotential of 230 mV at a current density of 100 mA cm-2 , indicating its high catalytic efficiency. Furthermore, the catalyst exhibits a Tafel slope of 26 mV dec-1 , suggesting favorable reaction kinetics. The CoNiAl0.5 O/NF catalyst exhibits impressive stability, ensuring its potential for practical applications. Detailed characterizations reveal that the enhanced activity of CoNiAl0.5 O/NF can be attributed to the electronic modulation achieved through Al3+ incorporation, which promotes the emergence of higher-valence Ni metal, facilitating nanofilament formation and improving mass transport and charge transfer processes. The synergistic effect between nanofilaments and porous nickel foam (NF) substrate significantly enhances the electrical conductivity of this catalyst material. This study highlights the significance of electronic structures for improving the activity of cost-effective and non-precious metal-based electrocatalysts for the OER., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)- Published
- 2024
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4. Tuning Octahedron Sites of CoV 2 O 4 via Cationic Competition for Efficient Oxygen Evolution Reaction.
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Lv YH, Wei S, Yi SS, Duan YX, Cui RC, Yang G, Liu ZY, Chen JH, and Yue XZ
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Doping transition metal oxide spinels with metal ions represents a significant strategy for optimizing the electronic structure of electrocatalysts. Herein, a bimetallic Fe and Ru doping strategy to fine-tune the crystal structure of CoV
2 O4 spinel for highly enhanced oxygen evolution reaction (OER) is presented performance. The incorporation of Fe and Ru is observed at octahedral sites within the CoV2 O4 structure, effectively modulating the electronic configuration of Co. Density functional theory calculations have confirmed that Fe acts as a novel reactive site, replacing V. Additionally, the synergistic effect of Fe, Co, and Ru effectively optimizes the Gibbs free energy of the intermediate species, reduces the reaction energy barrier, and accelerates the kinetics toward OER. As expected, the best-performing CoVFe0.5 Ru0.5 O4 displays a low overpotential of 240 mV (@10 mA cm-2 ) and a remarkably low Tafel slope of 38.9 mV dec-1 , surpassing that of commercial RuO2 . Moreover, it demonstrates outstanding long-term durability lasting for 72 h. This study provides valuable insights for the design of highly active polymetallic spinel electrocatalysts for energy conversion applications., (© 2024 Wiley‐VCH GmbH.)- Published
- 2024
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5. Decoction derived from Allium ascalonicum L. bulbs and Sojae Semen Praeparatum alleviates wind-cold-type common cold via Nrf2/HO-1 pathway and modulation of Lactobacillus murinus level.
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Jiang Y, Wei W, Zhou J, Qiu S, Yang Q, Huo JH, and Wang W
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Background: Cong-Chi decoction (CCD) is made using Allium ascalonicum L. (shallot) bulbs and Sojae Semen Praeparatum (SSP). Shallot bulbs and SSP are both used regularly in traditional Chinese medicine; however, there are no recent pharmacological studies on their synergistic effects. Despite their roles in the treatment of the common cold for thousands of years, their pharmacological mechanisms of action against wind-cold-type common cold are yet to be explored comprehensively., Methods: A mouse model was standardized using wind-cold modeling equipment to study the anti-inflammatory, antioxidant, and antiapoptotic effects of CCD. Then, 16S rRNA sequencing was employed to analyze the association between Lactobacillus murinus and changes in body temperature. Additionally, the antipyretic effects of L. murinus were validated via animal experiments., Results: The results indicate that CCD improves the symptoms of wind-cold by reducing fever, levels of pro-inflammatory factors, and cellular apoptosis, as well as increasing the blood leukocyte and lymphocyte counts, thereby alleviating lung tissue damage. The effects of CCD are mediated by upregulation of pulmonary Nrf2 and HO-1 expressions, thereby reducing oxidative damage in the lungs, in addition to other anti-inflammatory mechanisms. Furthermore, CCD increases the abundance of L. murinus in the intestinal tract. The animal experiments confirm that L. murinus ameliorates fever in mice., Conclusion: CCD exhibits remarkable antioxidant and anti-inflammatory properties for effectively treating wind-cold-type common cold. Furthermore, its regulatory effects on L. murinus represent a novel mechanism for product development., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Jiang, Wei, Zhou, Qiu, Yang, Huo and Wang.)
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- 2024
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6. Visible-Light-Driven Inactivation of Bacteria and H 2 Generation Catalyzed by Oxygen-Vacancy-Rich One-Dimensional/Two-Dimensional W 18 O 49 /g-C 3 N 4 Z-Scheme Heterostructures.
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Li CQ, Guo RF, Yi WJ, Wang SJ, Du X, Liu Y, Liu ZY, Chen JH, and Yue XZ
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- Oxygen Isotopes, Catalysis, Bacteria, Light
- Abstract
Z-scheme heterostructure-based photocatalysts consist of a reduction photocatalyst and an oxidation photocatalyst, enabling them to possess a high capacity for both reduction and oxidation. However, the coupling reaction between photocatalytic H
2 generation through water reduction and sterilization using Z-scheme systems has been rarely reported. Herein, 1D W18 O49 nanowires embedded over 2D g-C3 N4 nanosheets are well-constructed as an integrated Z-scheme heterojunction. Experimental results and density functional theory calculations not only demonstrate the achievement of efficient interfacial charge separation and transport, leading to prolonged lifetime of photogenerated charge carriers, but also directly confirm the mechanism of Z-scheme charge transfer. As expected, the optimized W18 O49 /g-C3 N4 nanostructure exhibits superior photocatalytic sterilization activity against Staphylococcus aureus as well as excellent H2 generation performance under visible-light irradiation (λ ≥ 420 nm). Due to its nontoxic nature, W18 O49 /g-C3 N4 holds great potential in eradicating bacterial infections in living organisms.- Published
- 2024
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7. Effect of acute normovolemic hemodilution on anesthetic effect, plasma concentration, and recovery quality in elderly patients undergoing spinal surgery.
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Liu T, Bai Y, Yin L, Wang JH, Yao N, You LW, and Guo JR
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- Aged, Humans, Hemodilution, Preoperative Care, Propofol, Anesthetics
- Abstract
Objective: To explore the effect of acute normovolemic hemodilution (ANH) on the anesthetic effect, plasma concentration, and postoperative recovery quality in elderly patients undergoing spinal surgery., Methods: A total of 60 cases of elderly patients aged 65 to 75 years who underwent elective multilevel spinal surgery were assigned randomly into the ANH group (n = 30) and control group (n = 30). Hemodynamic and blood gas analysis indexes were observed and recorded before ANH (T
1 ), after ANH (T2 ), immediately after postoperative autologous blood transfusion (T3 ), 10 min (T4 ), 20 min (T5 ), 30 min (T6 ), 40 min (T7 ), and 50 min (T8 ) after the transfusion, and at the end of the transfusion (i.e., 60 min; T9 ). At T3 ~ 9 , bispectral index (BIS) and train-of-four (TOF) stimulation were recorded and the plasma propofol/cisatracurium concentration was determined. The extubation time and recovery quality were recorded., Results: The ANH group presented a lower MAP value and a higher SVV value at T2 , and shorter extubation and orientation recovery time (P < 0.05) compared with the control group. BIS values at T8 and T9 were lower in the ANH group than those in the control group (P < 0.05). TOF values at T7 ~ 9 were lower in the ANH group than those in the control group (P < 0.05). There were no statistically significant differences in the postoperative plasma concentrations of propofol and cisatracurium between the groups (P > 0.05)., Conclusion: During orthopedic surgery, the plasma concentration of elderly patients is increased after autologous blood transfusion of ANH, and the depth of anesthesia and muscle relaxant effect are strengthened, thus leading to delayed recovery of respiratory function and extubation., (© 2023. BioMed Central Ltd., part of Springer Nature.)- Published
- 2023
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8. Reduced Vrk2 expression is associated with higher risk of depression in humans and mediates depressive-like behaviors in mice.
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Yin MY, Guo L, Zhao LJ, Zhang C, Liu WP, Zhang CY, Huo JH, Wang L, Li SW, Zheng CB, Xiao X, Li M, Wang C, and Chang H
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- Humans, Mice, Animals, Depression genetics, Polymorphism, Single Nucleotide, Protein Serine-Threonine Kinases metabolism, Genome-Wide Association Study, Leukemia, Myeloid, Acute
- Abstract
Background: Genome-wide association studies (GWAS) have reported single-nucleotide polymorphisms (SNPs) in the VRK serine/threonine kinase 2 gene (VRK2) showing genome-wide significant associations with major depression, but the regulation effect of the risk SNPs on VRK2 as well as their roles in the illness are yet to be elucidated., Methods: Based on the summary statistics of major depression GWAS, we conducted population genetic analyses, epigenome bioinformatics analyses, dual luciferase reporter assays, and expression quantitative trait loci (eQTL) analyses to identify the functional SNPs regulating VRK2; we also carried out behavioral assessments, dendritic spine morphological analyses, and phosphorylated 4D-label-free quantitative proteomics analyses in mice with Vrk2 repression., Results: We identified a SNP rs2678907 located in the 5' upstream of VRK2 gene exhibiting large spatial overlap with enhancer regulatory marks in human neural cells and brain tissues. Using luciferase reporter gene assays and eQTL analyses, the depression risk allele of rs2678907 decreased enhancer activities and predicted lower VRK2 mRNA expression, which is consistent with the observations of reduced VRK2 level in the patients with major depression compared with controls. Notably, Vrk2
-/- mice exhibited depressive-like behaviors compared to Vrk2+/+ mice and specifically repressing Vrk2 in the ventral hippocampus using adeno-associated virus (AAV) lead to consistent and even stronger depressive-like behaviors in mice. Compared with Vrk2+/+ mice, the density of mushroom and thin spines in the ventral hippocampus was significantly altered in Vrk2-/- mice, which is in line with the phosphoproteomic analyses showing dysregulated synapse-associated proteins and pathways in Vrk2-/- mice., Conclusions: Vrk2 deficiency mice showed behavioral abnormalities that mimic human depressive phenotypes, which may serve as a useful murine model for studying the pathophysiology of depression., (© 2023. The Author(s).)- Published
- 2023
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9. Identification of a psychiatric risk gene NISCH at 3p21.1 GWAS locus mediating dendritic spine morphogenesis and cognitive function.
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Yang ZH, Cai X, Ding ZL, Li W, Zhang CY, Huo JH, Zhang Y, Wang L, Zhang LM, Li SW, Li M, Zhang C, Chang H, and Xiao X
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- Humans, Mice, Animals, Cognition, Polymorphism, Single Nucleotide genetics, Morphogenesis, RNA, Messenger, Dendritic Spines, Genome-Wide Association Study methods
- Abstract
Background: Schizophrenia and bipolar disorder (BD) are believed to share clinical symptoms, genetic risk, etiological factors, and pathogenic mechanisms. We previously reported that single nucleotide polymorphisms spanning chromosome 3p21.1 showed significant associations with both schizophrenia and BD, and a risk SNP rs2251219 was in linkage disequilibrium with a human specific Alu polymorphism rs71052682, which showed enhancer effects on transcriptional activities using luciferase reporter assays in U251 and U87MG cells., Methods: CRISPR/Cas9-directed genome editing, real-time quantitative PCR, and public Hi-C data were utilized to investigate the correlation between the Alu polymorphism rs71052682 and NISCH. Primary neuronal culture, immunofluorescence staining, co-immunoprecipitation, lentiviral vector production, intracranial stereotaxic injection, behavioral assessment, and drug treatment were used to examine the physiological impacts of Nischarin (encoded by NISCH)., Results: Deleting the Alu sequence in U251 and U87MG cells reduced mRNA expression of NISCH, the gene locates 180 kb from rs71052682, and Hi-C data in brain tissues confirmed the extensive chromatin contacts. These data suggested that the genetic risk of schizophrenia and BD predicted elevated NISCH expression, which was also consistent with the observed higher NISCH mRNA levels in the brain tissues from psychiatric patients compared with controls. We then found that overexpression of NISCH resulted in a significantly decreased density of mushroom dendritic spines with a simultaneously increased density of thin dendritic spines in primary cultured neurons. Intriguingly, elevated expression of this gene in mice also led to impaired spatial working memory in the Y-maze. Given that Nischarin is the target of anti-hypertensive agents clonidine and tizanidine, which have shown therapeutic effects in patients with schizophrenia and patients with BD in preliminary clinical trials, we demonstrated that treatment with those antihypertensive drugs could reduce NISCH mRNA expression and rescue the impaired working memory in mice., Conclusions: We identify a psychiatric risk gene NISCH at 3p21.1 GWAS locus influencing dendritic spine morphogenesis and cognitive function, and Nischarin may have potentials for future therapeutic development., (© 2023. The Author(s).)
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- 2023
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10. An efficient mixed-valence copper pyrazolate catalyst for the conversion of carbon dioxide and epoxides into cyclic carbonates.
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Wang JG, Liu Y, Liu CM, Chen JH, and Yang G
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In this paper, a cyclic (Cu
I pz)3 ·CH3 CN (1) precursor and a mixed-valence pentanuclear complex Cu I3 CuII2(OH)pz6 ·CH3 CN (2) have been synthesized and structurally characterized by single-crystal X-ray diffraction, where pzH = 4-chloro-3,5-diphenylpyrazole. The excellent catalytic activity of 2 has been demonstrated in the chemical fixation of CO2 into value-added cyclic carbonates, which can be carried out at ambient pressure and room temperature along with ultra-high yield and perfect steric hindrance tolerance. Based on density functional theory (DFT) calculations and comparison with the catalytic performance of 1, it is proposed that the coordinatively unsaturated CuII atoms of 2 are probably the active sites for this catalytic reaction.- Published
- 2023
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11. Autologous blood transfusion promotes autophagy and inhibits hepatocellular carcinoma progression through HIF-1α signalling pathway.
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Bai Y, Liu T, Cui YH, Li ZZ, Zhou XF, Cheng Y, Wang JH, and Guo JR
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- Rats, Animals, Blood Transfusion, Autologous, Signal Transduction, Autophagy, Hypoxia-Inducible Factor 1, alpha Subunit genetics, Hypoxia-Inducible Factor 1, alpha Subunit metabolism, Cell Line, Tumor, Carcinoma, Hepatocellular pathology, Liver Neoplasms pathology
- Abstract
To explore the molecular mechanism of autologous blood transfusion promoting autophagy of hepatocellular carcinoma (HCC) cells and inhibiting the HCC progression through HIF-1α signalling pathway. This is a research paper. Rat hepatocellular carcinoma model and HepG2 cell model were built. The rats with HCC were conducted a surgery, and their blood was collected for detection to detect the recurrence and metastasis of the rats. Western blot was used to analysed the expression of HIF-1α, TP53, MDM2, ATG5 and ATG14 protein. The apoptosis rate of HepG2 cells was detected by flow cytometry, and autophagosomes were observed by transmission electron microscopy. HIF-1α expression was measured by immunofluorescence assay. The expressions of HIF-1α, TP53, MDM2, ATG5 and ATG14 protein were highest in model + autoblood group compared with the model group. HIF-1α content of model group was higher, but content of TP53, MDM2, ATG5 and ATG14 in the model group is the second. The highest apoptosis rate was found in HepG2 + autoblood group. The number of autophagosomes in HepG2 + autoblood was obviously larger than that of HepG2 + autoblood + inhibitor. HIF-1α expression of immunofluorescence assay showed that high expression of HIF-1α was clearly observed in HepG2 and HepG2 + autoblood group from confocal observation. However, there was no HIF-1α protein expression in HepG2 + autoblood + inhibitor group. The migration rate in HepG2 group, HepG2 + autoblood group and HepG2 + autoblood + inhibitor group was 85.71 ± 7.38%, 14.36 ± 6.54% and 61.25 ± 5.39%, respectively. Autologous blood transfusion promotes autophagy of HCC cells through HIF-1α signalling pathway, which further inhibits HCC migration and erosion., (© 2023 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.)
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- 2023
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12. The effect of parecoxib sodium on postoperative delirium in elderly patients with hip arthroplasty.
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Wang JH, Liu T, Bai Y, Chen YQ, Cui YH, Gao XY, and Guo JR
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Objective: This study aimed to clarify the effect of parecoxib sodium on the occurrence of postoperative delirium and to investigate its possible mechanism. Methods: A total of 80 patients who underwent elective hip arthroplasty in our hospital between December 2020 and December 2021 were selected and randomly divided into two groups: a parecoxib sodium group (group P, n = 40) and a control group (group C, n = 40). Patients in group P were intravenously injected with 40 mg of parecoxib sodium 30 min before anesthesia and at the end of the surgery. Patients in group C were intravenously injected with the same volume of normal saline at the same time points. The primary endpoint was the incidence of POD, and the secondary endpoints were the levels of inflammatory factors (tumor necrosis factor- α [TNF-α], interleukin [IL]-1β, IL-6, and IL-10), nerve injury-related factors (brain-derived neurotrophic factor [BDNF], S-100β protein, neuron-specific enolase [NSE], and neurofilament light chain [NfL]), and antioxidant factors (heme oxygenase-1 [HO-1]), as well as the Visual Analogue Scale (VAS) and Confusion Assessment Method-Chinese Reversion (CAM-CR) scores. Results: The incidence of POD was 10% in group P and 27.5% in group C. Intergroup comparison revealed that the levels of TNF-α, IL-1β, S-100β, NfL, and NSE were lower, and BDNF was higher, in group P than in group C at each postoperative time point. The levels of IL-6 were lower, and the levels of IL-10 and HO-1 were higher, in group P than in group C at 1 h and 1 day postoperatively ( p < 0.05). Three days after surgery, the differences in the levels of IL-6, IL-10, and HO-1 were not statistically significant between the two groups ( p > 0.05). The VAS and CAM-CR scores were lower at each postoperative time point in group P than in group C ( p < 0.05). Conclusion: Parecoxib sodium could reduce postoperative pain, decrease the plasma levels of inflammatory and nerve injury-related factors, upregulate HO-1 levels, and reduce the incidence of POD. The results of this study suggest that parecoxib sodium may reduce the occurrence of POD through the effects of anti-inflammation, analgesia, and antioxidants., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Wang, Liu, Bai, Chen, Cui, Gao and Guo.)
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- 2023
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13. Common and rare variants within SP4 exert distinct molecular mechanisms contributing to the risk of schizophrenia.
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Zhang Q, Huo JH, Guo L, Wang L, Wang C, and Li M
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- Humans, Genetic Predisposition to Disease, Schizophrenia genetics, Sp4 Transcription Factor genetics
- Abstract
Competing Interests: Declaration of Competing Interest The authors have no conflicts of interest to declare.
- Published
- 2022
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14. Bidirectional genetic overlap between bipolar disorder and intelligence.
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Shang MY, Wu Y, Zhang CY, Qi HX, Zhang Q, Huo JH, Wang L, Wang C, and Li M
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- Humans, Genome-Wide Association Study, Intelligence genetics, Brain, Alleles, Bipolar Disorder genetics
- Abstract
Background: Bipolar disorder (BD) is a highly heritable psychiatric illness exhibiting substantial correlation with intelligence., Methods: To investigate the shared genetic signatures between BD and intelligence, we utilized the summary statistics from genome-wide association studies (GWAS) to conduct the bivariate causal mixture model (MiXeR) and conjunctional false discovery rate (conjFDR) analyses. Subsequent expression quantitative trait loci (eQTL) mapping in human brain and enrichment analyses were also performed., Results: Analysis with MiXeR suggested that approximately 10.3K variants could influence intelligence, among which 7.6K variants were correlated with the risk of BD (Dice: 0.80), and 47% of these variants predicted BD risk and intelligence in consistent allelic directions. The conjFDR analysis identified 37 distinct genomic loci that were jointly associated with BD and intelligence with a conjFDR < 0.01, and 16 loci (43%) had the same directions of allelic effects in both phenotypes. Brain eQTL analyses found that genes affected by the "concordant loci" were distinct from those modulated by the "discordant loci". Enrichment analyses suggested that genes related to the "concordant loci" were significantly enriched in pathways/phenotypes related with synapses and sleep quality, whereas genes associated with the "discordant loci" were enriched in pathways related to cell adhesion, calcium ion binding, and abnormal emotional phenotypes., Conclusions: We confirmed the polygenic overlap with mixed directions of allelic effects between BD and intelligence and identified multiple genomic loci and risk genes. This study provides hints for the mesoscopic phenotypes of BD and relevant biological mechanisms, promoting the knowledge of the genetic and phenotypic heterogeneity of BD. The essential value of leveraging intelligence in BD investigations is also highlighted., (© 2022. The Author(s).)
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- 2022
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15. A functional population-specific variant rs77416373 in the Ca V 2.1 gene is associated with antidepressant treatment response in Han Chinese subjects with major depressive disorder.
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Zhang Y, Zhang CY, Li SW, Yuan J, Xu L, Wei YJ, Zhou F, Wang JY, Huo JH, Wang L, Feng LM, Kang CY, and Yang JZ
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- Antidepressive Agents pharmacology, Antidepressive Agents therapeutic use, Calcium Channels, N-Type, China, Humans, Depressive Disorder, Major drug therapy, Depressive Disorder, Major genetics
- Abstract
Competing Interests: Conflict of interest The authors have no conflicts of interest to declare.
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- 2022
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16. Two new terpenoids with anti-inflammatory activity from the fruits of Arenga pinnata (Wurmb) Merr.
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Wu JT, Algradi AM, Liu Y, Huo JH, Li XM, Yang BY, and Wang WM
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- Mice, Animals, Fruit chemistry, Anti-Inflammatory Agents chemistry, RAW 264.7 Cells, Terpenes pharmacology, Terpenes analysis, Arecaceae chemistry
- Abstract
Two new terpenoids ( 1 and 2 ), arenterpenoid D ( 1 ) and pinnasesquiterpene A ( 2 ), along with 16 phenylpropanoids ( 3 - 18 ) and 8 known terpenoids ( 19 - 26 ) were isolated from 70% EtOH extract of the Arenga pinnata (Wurmb) Merr. fruits. Their structures were elucidated by spectroscopic methods including HR-ESI-MS, 1D, and 2D NMR. The absolute configuration of arenterpenoid D ( 1 ) was defined by X-ray crystallographic analysis. Furthermore, we evaluated the anti-inflammatory activity of all compounds, and outcomes showed that 2 and 21 exposed moderate suppressive effects against NO generation in lipopolysaccharide-stimulated RAW 264.7 cells.
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- 2022
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17. Dietary β-carotene and vitamin A and risk of Parkinson disease: A protocol for systematic review and meta-analysis.
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Wu LY, Chen JX, Chen GS, Gao H, Huo JH, Pang YF, and Gao QH
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- Ascorbic Acid, Cross-Sectional Studies, Humans, Meta-Analysis as Topic, Retrospective Studies, Risk Factors, Systematic Reviews as Topic, Vitamin A, Vitamin E, Parkinson Disease epidemiology, beta Carotene
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Background: The beneficial effects of dietary β-carotene and vitamin A on Parkinson disease (PD) have been confirmed, but some studies have yielded questionable results. Therefore, this meta-analysis investigated the effect of dietary β-carotene and vitamin A on the risk of PD., Methods: The following databases were searched for relevant paper: PubMed, Embase, Medline, Scopus, Cochrane Library, CNKI, Wanfang Med online, and Weipu databases for the relevant paper from 1990 to March 28, 2022. The studies included were as follows: β-carotene and vitamin A intake was measured using scientifically recognized approaches, such as food frequency questionnaire (FFQ); evaluation of odds ratios using OR, RR, or HR; β-carotene and vitamin A intake for three or more quantitative categories; and PD diagnosed by a neurologist or hospital records., Results: This study included 11 studies (four cohort studies, six case-control studies, and one cross-sectional study). The high β-carotene intake was associated with a significantly lower chance of developing PD than low β-carotene intake (pooled OR = 0.83, 95%CI = 0.74-0.94). Whereas the risk of advancement of PD was not significantly distinctive among the highest and lowest vitamin A intake (pooled OR = 1.08, 95%CI = 0.91-1.29)., Conclusions: Dietary β-carotene intake may have a protective effect against PD, whereas dietary vitamin A does not appear to have the same effect. More relevant studies are needed to include into meta-analysis in the further, as the recall bias and selection bias in retrospective and cross-sectional studies cause misclassifications in the assessment of nutrient intake., Competing Interests: The authors have no conflicts of interest to disclose., (Copyright © 2022 the Author(s). Published by Wolters Kluwer Health, Inc.)
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- 2022
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18. Exploring the mechanism of Qinbaiqingfei-concentrate pills in the treatment of Mycoplasma pneumoniae pneumonia from the perspective of intestinal microbiota and mucosal immunity.
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Liu Z, Dong WT, Wei WF, Huo JH, and Wang WM
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- Animals, Immunoglobulin A, Secretory, Interleukin-10, Interleukin-4, Mycoplasma pneumoniae, NF-kappa B metabolism, Rats, Tumor Necrosis Factor-alpha, Drugs, Chinese Herbal therapeutic use, Gastrointestinal Microbiome, Immunity, Mucosal, Pneumonia, Mycoplasma drug therapy
- Abstract
Ethnopharmacological Relevance: Traditional Chinese medicine categorizes Mycoplasma pneumoniae pneumonia as "lung heat", and treatment with heat clear and detoxify. Traditional Chinese medicine believes that the lungs and intestines come from the same source, and the intestine is related to pneumonia. This is the same as the gut-lung axis theory. Qinbaiqingfei concentrate pills (QBs) were modified based on Cough San in the ancient medical book Medical Awareness. It clears lung heat, moisturizes the lungs and dredges collaterals, and has a good ability to treat Mycoplasma pneumoniae., Aim of the Study: A rat model of Mycoplasma pneumoniae was established. From the aspect of intestinal flora and mucosal immunity, the potential mechanism of the QBs was researched., Materials and Methods: First, the content of Mycoplasma pneumoniae in lung tissue and the levels of the inflammatory factors IL-4, IL-10, TNF-α and INF-γ were detected. To determine the expression of NF-kB related proteins in lung tissue, which can understand the ability in treating disease. Next, metagenomic sequencing was performed to detect changes in short-chain fatty acids, proving the ability of the drug to regulate intestinal microecology. Finally, HDAC, LPS, SIgA, etc. were detected to facilitate the correlation of the overall experimental indicators., Results: QBs reduces the levels of IL-4, IL-10, TNF-α and INF-γ in the serum by inhibiting the expression of MyD88, IKKα, IκBα, and NF-κB p65 in lung tissue. In addition, QBs restores the ratio of gram-negative bacteria to gram-positive bacteria in the intestine, restores the secretion of acetic acid, propionic acid, butyric acid, isobutyric acid and isovaleric acid, and promotes the secretion of NF-κB p65 and SIgA by HDAC1/3. The result is that the lung tissue is repaired and the proliferation of Mycoplasma pneumoniae is inhibited., Conclusions: From the "gut-lung axis", a new research perspective was discovered. QBs intervened in the intestines and lungs to treat Mycoplasma pneumoniae., (Copyright © 2022 Elsevier B.V. All rights reserved.)
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- 2022
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19. [Therapeutic material basis of Daqinglong Decoction: based on serum pharmacochemistry and network pharmacology].
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Yuan M, Sun GD, Liu HS, Huo JH, and Wang WM
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- Chromatography, High Pressure Liquid, Molecular Docking Simulation, Network Pharmacology, Drugs, Chinese Herbal analysis, Drugs, Chinese Herbal pharmacology, Tandem Mass Spectrometry
- Abstract
Based on the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) technology, the components of Daqinglong Decoction absorbed in serum were analyzed and identified, and the therapeutic material basis of the prescription was revealed via network pharmacology. UPLC conditions are as follows: Waters Acquity UPLC BEH C_(18) column(2.1 mm × 100 mm, 1.7 μm), mobile phase of 0.1% formic acid aqueous solution(A)-0.1% formic acid in acetonitrile(B), gradient elution. Peakview 2.0 and MetabolitePilot 1.5 were employed for the comparison of Daqinglong Decoction, blank serum, and serum after the administration of the decoction, and the components of Daqinglong Decoction absorbed in serum were analyzed based on MS/MS profiles in related database and literature. The targets of the components absorbed in serum were retrieved from SwissTargetPrediction, DrugBank, and Batman-TCM. With the search terms of common cold, influenza, flu, bronchitis, bronchiolitis, asthma, allergic rhinitis, rhinallergosis, allergic coryza, rheumatic arthritis, and nephritis, the related disease targets were screened out. Then the absorbed component-potential target gene network and absorbed component target-disease target network were constructed, followed by Gene Ontology(GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis of the core targets. iGEMDOCK was employed for molecular docking of the absorbed components and core targets. In the serum after the administration of the decoction, 28 components were preliminarily identified, with 21 prototypes and 7 metabolites. Among them, 5 core components of ephedrine, demethylephedrine, glycyrrhetinic acid, p-hydroxybenzoic acid, and 2-methoxybenzoic acid were screened out, and 9 core targets, such as JUN, tumor protein 53(TP53), and protein kinase B(AKT1), were identified. Molecular docking showed high binding affinity of core components and core targets. Therefore, Daqinglong Decoction may exert therapeutic effect by regulating mitogen-activated protein kinase(MAPK), cyclic adenosine monophosphate(cAMP), and cyclic guanosine monophosphate(cGMP)-protein kinase G(PKG) signaling pathways and further improving and regulating inflammatory response and other physiological and pathological processes. This study clarifies the components of Daqinglong Decoction absorbed in serum and explores the therapeutic material basis of the prescription, which provides a reference for further elucidating the mechanism of Daqinglong Decoction and its clinical application.
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- 2022
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20. [Mechanism of Qingwei Powder in treatment of periodontitis based on UPLC-Q-TOF-MS, GC-MS, network pharmacology and molecular docking].
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Meng Y, Jiang ZT, Yan GJ, Shen J, Sun KP, Wang YY, Cao JN, Xia MY, and Pan JH
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- Gas Chromatography-Mass Spectrometry, Humans, Medicine, Chinese Traditional, Molecular Docking Simulation, Network Pharmacology, Powders, Drugs, Chinese Herbal pharmacology, Drugs, Chinese Herbal therapeutic use, Periodontitis drug therapy
- Abstract
The present study explored the mechanism of Qingwei Powder(QP) in the treatment of periodontitis based on chromatography-mass spectrometry and network pharmacology-molecular docking techniques. UPLC-Q-TOF-MS and GC-MS were used to identify the chemical constituents of QP. The active components and targets were screened out through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP), and their targets were predicted using SwissTargetPrediction. Targets related to periodontitis were obtained from GeneCards, OMIM, and DisGeNET. Venn diagram was constructed using Venny 2.1 to obtain the intersection targets. Cytoscape 3.7.2 was used to construct the "chemical component-target-disease" network. The targets were analyzed for Gene Ontology(GO) function and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment by clusterProfiler R, and the "chemical component-target-pathway" network was constructed. The binding activity of the active components to the target proteins was verified by molecular docking. A total of 189 chemical components were obtained by UPLC-Q-TOF-MS and GC-MS, including 39 active components with 180 potential targets related to periodontitis. Target enrichment analysis of the active components yielded 92 KEGG pathways. Twenty KEGG pathways, 34 active components, and 99 targets were involved in the "chemical component-target-pathway" network. Molecular docking verified a good binding ability of the key targets to the key compounds. This study preliminarily indicates that QP is effective in treating periodontitis through multiple components, multiple targets, and multiple pathways, which reflects the complex system of Chinese medicine. This study provides the theoretical foundation for the subsequent research on the material basis and key quality attributes of QP.
- Published
- 2022
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21. [Quality value transmitting of substance benchmarks in Danggui Buxue Decoction].
- Author
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Zhuang XY, Zhang Q, Qi YL, Bai YL, Li WL, Pan JH, Mao CQ, Chen J, and Yan GJ
- Subjects
- Chromatography, High Pressure Liquid methods, Drugs, Chinese Herbal standards, Quality Control
- Abstract
To clarify the key quality attributes of substance benchmarks in Danggui Buxue Decoction(DBD), this study prepared 21 batches of DBD substance benchmarks, and established two methods for detecting their fingerprints, followed by the identification of peak attribution and similarity range as well as the determination of extract and transfer rate ranges and contents of index components ferulic acid, calycosin-7-O-β-D-glucoside, and astragaloside Ⅳ. The mass fractions and transfer rates of DBD substance benchmarks from different batches were calculated as follows: ferulic acid(index component in Angelicae Sinensis Radix): 0.037%-0.084% and 31.41%-98.88%; astragaloside Ⅳ(index component in Astragali Radix): 0.021%-0.059% and 32.18%-118.57%; calycosin-7-O-β-D-glucoside: 0.002%-0.023% and 11.51%-45.65%, with the extract rate being 18.4%-36.1%. The similarity of fingerprints among 21 batches of DBD substance benchmarks was all higher than 0.9. The quality control method for DBD substance benchmarks was preliminarily established based on the HPLC fingerprint analysis and index component determination, which has provided a basis for the subsequent development of DBD and the quality control of novel related preparations.
- Published
- 2022
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22. The Effect of Pre-operative Autologous Blood Donation on Bone Marrow Hematopoietic Functions in Rabbits after Hepatectomy.
- Author
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Zhou XF, Liu Y, Xu JM, Wang JH, Li ZZ, Zhou X, and Guo JR
- Subjects
- Animals, Blood Transfusion, Autologous, Flow Cytometry, Hepatectomy, Humans, Rabbits, Blood Donors, Bone Marrow
- Abstract
Background: Pre-operative autologous blood donation (PABD) is one of the most widely distributed autologous blood donation means, which has positive effects on erythropoiesis. However, whether PABD can stimulate the bone marrow hematopoiesis after hepatectomy has not been reported., Methods: Totally 80 New Zealand rabbits were randomly divided into 4 groups that included control group, surgery group, hemodilutional autotransfusion (HA) group and PABD group. Automatic reticulocyte examination was performed to detect the content of reticulocyte and immature reticulocyte fractions (IRF). Flow cytometric analysis was employed to monitor the level of CD34+ cells and the cell cycle status. Southern blotting was conducted to determine the telomere length of CD34+ cells., Results: The content of high fluorescence reticulocytes (HFR) and IRF was decreased at 6 h and 24 h after autotransfusion. However, the level of CD34+ cells was upregulated after PABD. Cell cycle status analysis revealed that the majority of the CD34+ cells in HA and PABD group were maintained in G0/G1 phase. The telomere length in HA and PABD group was shortened than that of the control group and surgery group., Conclusion: PABD could promote the bone marrow hematopoietic functions in rabbits after hepatectomy via stimulating proliferation of CD34+ cells and shortening the telomere length of CD34+ cells, but the content of HFR was not increased immediately because of the stuck of CD34+ cells in the G0/G1 phase., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
- Published
- 2022
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23. A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia.
- Author
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Liu Z, Huo JH, Dong WT, Sun GD, Li FJ, Zhang YN, Qin ZW, Pengna J, and Wang WM
- Abstract
Qinbaiqingfei concentrated pills (QB) are a commonly used medicine for the treatment of mycoplasma pneumonia in China, and the mechanism of action of QB needs to be studied further. Therefore, we use a combination of metabolomics and network pharmacology to clarify the mechanism of QB. Nontarget metabolomics studies were performed on rat serum, urine, and lung tissues, and 56 therapeutic biomarkers were found. Subsequently, the components of QB absorbed into the blood and lung tissues were clarified, and based on this finding, the core target of network pharmacology was predicted. The enrichment analysis of biomarkers-genes finally confirmed their close relationship with the NF-κB signaling pathway. By western blotting expression of the proteins in the lung tissue-related signaling pathways, it is finally confirmed that QB inhibits the NF-κB signaling pathway through SIRT1, IL-10 and MMP9, CTNNB1, EGFR, and other targets. It plays a role in regulating immunity, regulating metabolism, and treating diseases., Competing Interests: The authors declare that the research study was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Liu, Huo, Dong, Sun, Li, Zhang, Qin, pengna and Wang.)
- Published
- 2021
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24. The effect of erythrocyte transfusion on macrophage pyroptosis and inflammation in a sepsis model.
- Author
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Cheng Y, Li ZZ, Wang H, Wang JH, Zhou XF, Xu JM, Zhou X, and Guo JR
- Subjects
- Animals, China, Erythrocyte Transfusion, Inflammation, Macrophages, Rats, Pyroptosis, Sepsis
- Abstract
Background: Sepsis is one of most common causes of death in the intensive care unit (ICU) due to infection and inflammation. The Duffy antigen receptor for chemokines (DARC) regulates pro-inflammatory cytokines, thus playing an important role in inflammation., Objectives: This study aimed to elucidate the correlation among erythrocyte transfusion, macrophage pyroptosis and inflammation in the progression of sepsis., Material and Methods: Alanine aminotransferase (ALT/GPT) activity was measured with the ALT/GPT activity measurement kit (Jiancheng Bio, Nanjing, China) according to the kit manual. The ET-1 concentration was measured with enzyme-linked immunosorbent assay (ELISA) using the endothelin-1 (ET-1) measurement kit (Jiancheng Bio) according to the kit manual. Apoptosis was evaluated using flow cytometry-based Annexin V staining assay. The cells were collected using centrifugation and resuspended in binding buffer. Ultrastructural analysis of pyroptotic body, the levels of interleukin (IL)-1β, IL-18, IL-33, MIP-2, CXCL8, reactive oxygen species (ROS), and LTB4 were measured with ELISA., Results: Our results showed that septic rats had impaired hepatic function and ET-1 levels. Erythrocyte transfusion upregulated DARC expression in the sepsis model. Erythrocyte transfusion also affected pyroptosis in macrophages, reduced the production of inflammatory cytokines, such as IL-1β, IL-18 and IL-33, and alleviated cytotoxicity in the sepsis model., Conclusions: Erythrocyte transfusion may function as a therapeutic tool against sepsis by regulating pyroptosis, inflammation and cytotoxicity.
- Published
- 2021
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25. Complexation of triangular silver(I) or copper(I) nitropyrazolates with dibenzothiophenes having potential use in adsorptive desulfurization.
- Author
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Yang L, Wang L, Lv X, Chen JH, Wang Y, and Yang G
- Abstract
Triangular silver(i) and copper(i) 3,5-diethyl-4-nitropyrazolates (abbreviated as [Ag(denpz)]3 or Ag3pz3, and [Cu(denpz)]3 or Cu3pz3), as well as their adducts with dibenzothiophene (DBT), 4,6-dimethyldibenzothiophene (DMDBT) and benzothiophene (BT), have been prepared and characterized by a series of techniques. X-ray analyses show that these adducts are stabilized by MS, MC contacts and ππ stacking interactions. NMR measurements and theoretical calculations indicate that the intensity of interaction between the metal complexes and dibenzothiophenes follows the trend: Ag3pz3-DMDBT > Ag3pz3-DBT > Cu3pz3-DMDBT > Cu3pz3-DBT, which can be understood on the basis of a weak interaction between π-acid (Ag3pz3 or Cu3pz3) and π-base (DBT/DMDBT). Both complexes show good adsorptive ability and reusability toward the removal of DBT and DMDBT from model oil (n-octane), with the maximum adsorption capacity at room temperature being 39 mg S (DMDBT) per g Cu3pz3, 34 mg S (DMDBT) per g Ag3pz3, 40 mg S (DBT) per g Cu3pz3, 36 mg S (DBT) per g Ag3pz3, respectively. Compared to Ag3pz3, Cu3pz3 exhibits higher adsorptive capacities for DBT/DMDBT, which has been attributed to its lower molecular mass.
- Published
- 2021
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26. [Analysis of chemical constituents of Ginseng-Douchi compound fermentation products based on UPLC-Q-TOF-MS].
- Author
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Sun JF, Dong WT, Chen LY, Sun GD, Huo JH, and Wang WM
- Subjects
- Chromatography, High Pressure Liquid, Fermentation, Tandem Mass Spectrometry, Drugs, Chinese Herbal, Panax
- Abstract
In this experiment, ultra high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used to analyze and identify chemical constituents of Ginseng-Douchi(GD) compound fermentation, and explore the conversion rules of ginsenosides and soybean isoflavones after compound fermentation. Waters Acquity UPLC BEH C_(18) column(2.1 mm×100 mm, 1.7 μm) was adopted, with 0.1% formic acid aqueous solution(A)-0.1% formic acid acetonitrile solution(B) as mobile phase for gradient elution; electrospray ion source(ESI) was used to collect data in positive and negative ion modes; according to the exact mass number, the secondary spectrum comparison of the database and the existing literature reports, Peakview 2.0/masterview 1.0 software was used to determine the common ion structure formula. Finally, a total of 133 chemical constituents were analyzed and identified from the GD. Ginseng saponins and isoflavone glycosides were significantly converted after fermentation. Among them, peak areas of prototype ginsenosides Rk_3, Rh_1, Rh_2, Rh_3, daidzin, glycitin and genistin decreased significantly; whereas peak areas of se-condary ginsenoside Rb_1, Rb_2, Rk_1, glycitein, genistein and daidzein increased significantly. In this experiment, liquid-mass spectrometry technique was used to investigate the conversion of active ingredients of GD compound fermented products after co-fermentation, so as to provide a scientific basis for elucidating pharmacodynamics material basis and quality control.
- Published
- 2021
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27. Conversion of CO 2 to Heterocyclohexenol Carboxylic Acids through a Metal-Organic Framework Sponge.
- Author
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Wang ZQ, Chen JH, Qiu X, Ma JG, and Cheng P
- Abstract
The conversion of CO
2 into high value-added chemical products is the focus of current scientific research. We make use of the specific porous structure of nanosized metal-organic frameworks (MOFs) loading the highly active yet metastable nano Cu2 O to catalyze the conversion of CO2 into a series of high value-added bioactive pyridone/pyrone-3-carboxylic acid products via heterocyclic 4-hydroxy-2-pyridones/pyrones, which exhibit high activity, selectivity, and reusability. Nano MOF sponge-covered metastable nanoparticles (NPs) converting CO2 into high value-added bioproducts provide a facile "dual-side surfactant" strategy, a highly efficient composite catalyst, and a practicable pathway not only for the sustainable use of CO2 but also for environment-friendly production of bioproducts.- Published
- 2021
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28. Exploration on the effect of predeposit autotransfusion on bone marrow hematopoiesis after femoral shaft fracture.
- Author
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Li ZZ, Wang H, Jia DL, Wang JH, Xu JM, Ma L, and Guo JR
- Subjects
- Bone Marrow Cells, Hematopoiesis, Hematopoietic Stem Cells, Humans, Blood Transfusion, Autologous, Bone Marrow
- Abstract
Objective: By observing the changes in the number and activity of CD34+ cells in bone marrow after predeposit autotransfusion (PAT) to patients with femoral shaft fracture (FSF), to evaluate the effects of PAT on hematopoietic function and hematopoietic stem cells in bone marrow., Methods: Selected FSF patients were randomly divided into 2 groups: the control group (patients did not receive blood transfusion after surgery) and PAT group (patients received PAT after surgery). The content of RBC and Plt in blood samples were counted by blood routine. The cell cycle and proportion of CD34+ myelinated cells in blood samples was analyzed by flow cytometry. The telomere DNA length of hematopoietic stem cells (HSCs) in the control groups and PAT group at postoperation 24 was analyzed by southern blot., Results: The content of RBC and Plt in postoperation 6h and 24h in the control group was evidently higher compared to that in PAT group, while Hb content in control group was significantly lower compared to that in PAT group. The proportion of CD34+ myelinated cells in post-transfusion 6h and postoperation 24h in PAT group was evidently higher compared to that in the control group. In PAT group, S phase at postoperation 24h was significantly larger compared to that at post-transfusion 6h. The telomere DNA length of HSCs in PAT group was longer than that in the control group., Conclusion: PAT can increase the number of HSC, while does not cause the abnormal aging of HSCs. PAT is suitable for postoperative blood transfusion of patients with FSF., (Copyright © 2020. Published by Elsevier Masson SAS.)
- Published
- 2021
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29. Correction to: Cardiac rupture complicating acute myocardial infarction: the clinical features from an observational study and animal experiment.
- Author
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Lu Q, Liu P, Huo JH, Wang YN, Ma AQ, Yuan ZY, Du XJ, and Bai L
- Abstract
An amendment to this paper has been published and can be accessed via the original article.
- Published
- 2020
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30. Cardiac rupture complicating acute myocardial infarction: the clinical features from an observational study and animal experiment.
- Author
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Lu Q, Liu P, Huo JH, Wang YN, Ma AQ, Yuan ZY, Du XJ, and Bai L
- Subjects
- Aged, Aged, 80 and over, Animals, Disease Models, Animal, Female, Heart Rupture, Post-Infarction diagnosis, Heart Rupture, Post-Infarction mortality, Heart Rupture, Post-Infarction prevention & control, Hospital Mortality, Humans, Male, Mice, Inbred C57BL, Middle Aged, Myocardial Reperfusion, Retrospective Studies, Risk Assessment, Risk Factors, ST Elevation Myocardial Infarction diagnosis, ST Elevation Myocardial Infarction mortality, ST Elevation Myocardial Infarction therapy, Time Factors, Heart Rupture, Post-Infarction etiology, ST Elevation Myocardial Infarction complications
- Abstract
Background: Cardiac rupture (CR) is a fatal complication of ST-elevation myocardial infarction (STEMI) with its incidence markedly declined in the recent decades. However, clinical features of CR patients now and the effect of reperfusion therapy to CR remain unclear. We investigated the clinical features of CR in STEMI patients and the effect of reperfusion therapy to CR in mice., Methods: Two studies were conducted. In clinical study, data of 1456 STEMI patients admitted to the First Hospital, Xi'an Jiaotong University during 2015.12. ~ 2018.12. were analyzed. In experimental study, 83 male C57BL/6 mice were operated to induce MI. Of them, 39 mice were permanent MI (group-1), and remaining mice received reperfusion after 1 h ischemia (21 mice, group-2) or 4 h ischemia (23 mice, group-3). All operated mice were monitored up to day-10. Animals were inspected three times daily for the incidence of death and autopsy was done for all mice found died to determine the cause of death., Results: CR was diagnosed in 40 patients: free-wall rupture in 17, ventricular septal rupture in 20, and combined locations in 3 cases. CR presented in 19 patients at admission and diagnosed in another 21 patients during 1 ~ 14 days post-STEMI, giving an in-hospital incidence of 1.4%. The mortality of CR patients was high during hospitalization accounting for 39% of total in-hospital death. By multivariate logistic regression analysis, older age, peak CK-MB and peak hs-CRP were independent predictors of CR post-STEMI. In mice with non-reperfused MI, 17 animals (43.6%) died of CR that occurred during 3-6 days post-MI. In MI mice received early or delayed reperfusion, all mice survived to the end of experiment except one mouse died of acute heart failure., Conclusion: CR remains as a major cause of in-hospital death in STEMI patients. CR patients are characterized of being elderly, having larger infarct and more server inflammation. Experimentally, reperfusion post-MI prevented CR.
- Published
- 2020
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31. Altered resting-state functional connectivity and effective connectivity of the habenula in irritable bowel syndrome: A cross-sectional and machine learning study.
- Author
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Mao CP, Chen FR, Huo JH, Zhang L, Zhang GR, Zhang B, and Zhou XQ
- Subjects
- Adult, Cross-Sectional Studies, Dorsolateral Prefrontal Cortex diagnostic imaging, Feasibility Studies, Female, Habenula diagnostic imaging, Humans, Male, Middle Aged, Pain diagnostic imaging, Periaqueductal Gray diagnostic imaging, Thalamus diagnostic imaging, Young Adult, Connectome, Dorsolateral Prefrontal Cortex physiopathology, Habenula physiopathology, Irritable Bowel Syndrome diagnostic imaging, Irritable Bowel Syndrome physiopathology, Magnetic Resonance Imaging, Pain physiopathology, Periaqueductal Gray physiopathology, Support Vector Machine, Thalamus physiopathology
- Abstract
Irritable bowel syndrome (IBS) is a disorder involving dysfunctional brain-gut interactions characterized by chronic recurrent abdominal pain, altered bowel habits, and negative emotion. Previous studies have linked the habenula to the pathophysiology of negative emotion and pain. However, no studies to date have investigated habenular function in IBS patients. In this study, we investigated the resting-state functional connectivity (rsFC) and effective connectivity of the habenula in 34 subjects with IBS and 34 healthy controls and assessed the feasibility of differentiating IBS patients from healthy controls using a machine learning method. Our results showed significantly enhanced rsFC of the habenula-left dorsolateral prefrontal cortex (dlPFC) and habenula-periaqueductal grey (PAG, dorsomedial part), as well as decreased rsFC of the habenula-right thalamus (dorsolateral part), in the IBS patients compared with the healthy controls. Habenula-thalamus rsFC was positively correlated with pain intensity (r = .467, p = .005). Dynamic causal modeling (DCM) revealed significantly decreased effective connectivity from the right habenula to the right thalamus in the IBS patients compared to the healthy controls that was negatively correlated with disease duration (r = -.407, p = .017). In addition, IBS was classified with an accuracy of 71.5% based on the rsFC of the habenula-dlPFC, habenula-thalamus, and habenula-PAG in a support vector machine (SVM), which was further validated in an independent cohort of subjects (N = 44, accuracy = 65.2%, p = .026). Taken together, these findings establish altered habenular rsFC and effective connectivity in IBS, which extends our mechanistic understanding of the habenula's role in IBS., (© 2020 The Authors. Human Brain Mapping published by Wiley Periodicals, Inc.)
- Published
- 2020
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32. Tripterine Restrains the Aggressiveness of Hepatocellular Carcinoma Cell via Regulating miRNA-532-5p/CXCL2 Axis.
- Author
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Jiang ZT, Han Y, Liu XY, Lv LY, Pan JH, and Liu CD
- Abstract
Introduction: Triterpene has attracted considerable interests because it exhibits anticancer effects. However, the effects of tripterine on hepatocellular carcinoma (HCC) are not well studied. In the current study, the mechanism of tripterine on HCC cells growth and metastasis was examined., Methods: The inhibitory effect on the growth and aggressiveness in HCC cells was analyzed by Cell Counting Kit-8 (CCK-8), wound healing and Transwell assay. The levels of microRNA-532-5p (miR-532-5p) in HCC cells and tissues were measured using qRT-PCR. The expression of chemokine (C-X-C Motif) ligand 2 (CXCL2) was determined by Western blotting and immunohistochemistry (IHC). Luciferase reporter gene assay was used to validate the binding between miR-532-5p and CXCL2. The impact of tripterine on the growth and metastasis of HCC cells in vivo was analyzed using transplanted tumor model and experimental lung metastasis model, respectively., Results: We found that tripterine inhibited HCC cells proliferation, migration ability and invasion. Under tripterine treatment, the level of miR-532-5p was strikingly raised, and overexpression of miR‑532-5p reduced cell viability and metastatic-related traits. In addition, we identified CXCL2 as a target of miR-532-5p in HCC. Rescue experiments indicated that overexpression of CXCL2 restored the migration and invasive capacity of HCC cells inhibited by miR-532-5p or tripterine treatment. Finally, the tumor growth and metastatic ability of HCC MHCC97H cell in vivo were also significantly restrained by tripterine. The expression of CXCL2 was distinctly decreased and miR-532-5p level was increased by tripterine in vivo., Conclusion: In conclusion, tripterine inhibits the growth, migration ability and invasiveness of HCC cells through intervening miR-532-5p/CXCL2., Competing Interests: The authors report no conflicts of interest in this work., (© 2020 Jiang et al.)
- Published
- 2020
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33. A systematic investigation of structural transformation in a copper pyrazolato system: a case study.
- Author
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Chen JH, Wei D, Yang G, Ma JG, and Cheng P
- Abstract
Dissolution-recrystallization structural transformation (DRST) is a powerful tool to unravel unequivocally the mechanism of dynamic conversion processes, based on the structures of the initial reactants, final products and sometimes intermediates isolated from the reaction mixture. Herein, we illustrate the details of the conversion processes of (CuIpz)3 into [CuII(OH)pz]6 (pzH = 4-chloro-3,5-diphenylpyrazole) through DRSTs. Based on crystal structure determination and spectroscopic methods, the most encountered species, (CuIpz)3, is in equilibrium with (CuIpz)4 in solution with the tetramer becoming dominant at low temperature, indicating an entropy-controlled conversion between these two structural isomers. The CuI trimer/tetramer in solution further experiences an oxidation if exposed to the open air, resulting in the formation of a pentanuclear mixed-valence intermediate CuI3CuII2(OMe)2pz5 which can be isolated as single crystals at -20 °C and has been structurally characterized for the first time. The final product isolated from the solution is the fully oxidized hexanuclear [CuII(OMe)pz]6, which is easily transformed into [CuII(OH)pz]6 in the presence of humidity.
- Published
- 2020
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34. Plasma Transfusion Promoted Reprogramming CD4 + T Lymphocytes Immune Response in Severe Sepsis Mice Model Through Modulating the Exosome Protein Galectin 9.
- Author
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Zhang L, Zhang JP, Liu Y, Wang H, Cheng Y, Wang JH, Zhang WJ, Li ZZ, and Guo JR
- Subjects
- Animals, Disease Models, Animal, Mice, CD4-Positive T-Lymphocytes metabolism, Exosomes metabolism, Galectins metabolism, Plasma metabolism, Sepsis blood
- Abstract
Sepsis is a life-threatening disease that results in excessive stimulation of the host's immune cells. In the animal study, the purpose was to investigate the roles of fresh frozen plasma (FFP) transfusion in shaping the CD4
+ T lymphocytes immune response through modulating the secreted exosome protein Galectin-9 in mice with severe sepsis. By using Western blot analysis, we first identified that the protein Galectin-9 is highly accumulated in the blood plasma of severe sepsis mice, and with transmission electron microscopy (TEM) and protein analysis, we found that Galectin-9 is a secreted exosome protein. Thereafter, we treated the severe sepsis mice with the antibiotic Cefuroxime Axetil; one group of mice received FFP transfusion and the other group of mice received normal saline. Surprisingly, the FFP transfusion reduced the secretion of exosome protein Galectin-9 and there was crosstalking between the exosome protein Galectin-9 and CD4+ T lymphocytes in mice with severe sepsis. Results showed that the proliferation of T helper (Th) cells (Th1 and Th17) was promoted, and regulatory T (Treg) cells' maintenance was inhibited in the sepsis mice after receiving FFP transfusion. Correspondingly, this immune reprogrammed activity shaped the inflammatory cytokine secretion with an increase in the interleukin (IL)-1β, IL-6, and interferon-gamma levels, while it decreased IL-10 levels. Taken together, it was suggested that FFP transfusion promoted reprogramming of CD4+ T lymphocytes' immune response through inhibiting the secretion of exosome protein Galectin-9 in mice with severe sepsis to relieve immunosuppression.- Published
- 2020
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35. Adducts of triangular silver(i) 3,5-bis(trifluoromethyl)pyrazolate with thiophene derivatives: a weak interaction model of desulfurization.
- Author
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Liu R, Zhang W, Wei D, Chen JH, Ng SW, and Yang G
- Abstract
π-Acidic triangular silver(i) 3,5-bis(trifluoromethyl)pyrazolate (Ag
3 pz3 ) can form 1 : 1 adducts with dibenzothiophene (DBT), 4,6-dimethyldibenzothiophene (DMDBT), benzothiophene (BT), and 2,5-dimethylthiophene (DMT), which are stabilized by weak AgS and AgC contacts and sometimes by π-π stacking and, therefore, may represent a weak interaction model for some adsorptive desulfurization processes.- Published
- 2019
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36. New Diterpenes from Arenga pinnata (Wurmb.) Merr. Fruits.
- Author
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Liu JF, Huo JH, Wang C, Li FJ, Wang WM, and Huang LQ
- Subjects
- Carbon-13 Magnetic Resonance Spectroscopy, Diterpenes chemistry, Proton Magnetic Resonance Spectroscopy, Arecaceae chemistry, Diterpenes isolation & purification, Fruit chemistry
- Abstract
Three new ent -kauran-type diterpenes ( 1 ⁻ 3 ), named arenterpenoids A⁻C, and five known ones ( 4 ⁻ 8 ) were isolated and identified from Arenga pinnata (Wurmb.) Merr. Fruits. The structures of these compounds were established by 1D and 2D NMR spectra and HR-ESI-MS. To the best of our knowledge, this is the first scientific report of diterpenes from Arenga genus.
- Published
- 2018
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37. [Landscape pattern analysis and optimum design of park green space in Nanchang City, China based on GIS.]
- Author
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Wei XY, Cai JH, Ye YC, Zhou Y, and Liu CQ
- Subjects
- China, Cities, Ecosystem, City Planning methods, Conservation of Natural Resources methods, Geographic Information Systems
- Abstract
Based on the current map data of park green space in the main urban area of Nanchang, the spatial database of park green space was set up with GIS technique, with the corresponding landscape indices being calculated by FRAGSTATS, the software of landscape pattern. Based on analyzing current landscape pattern of green space in Nanchang, the optimization strategy and scheme were proposed and the optimized landscape pattern was evaluated. The results indicated that the spatial distribution of patches in current park green space was uneven and area discrepancy was large, which is especially true in densely populated areas with less patch number of park green space and obviously low available area for disaster shelter. By substantially increasing the quantity and area of patches, improving the inter-patch connectivity, and increasing landscape fragmentation index appropriately, the "point-line-plane" pattern of park green space system in Nanchang would be optimized and the spatial distribution would be more rational, which could effectively enhance its role in biodiversity conservation, disaster prevention, and risk avoidance. The optimized indices of patch diversity, evenness and aggregation would be significantly increased, the dominance index would be reduced, and the landscape diversity would be more abundant.
- Published
- 2018
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38. Perioperative Use of Levosimendan Improves Clinical Outcomes in Patients After Cardiac Surgery: A Systematic Review and Meta-Analysis.
- Author
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Qiang H, Luo X, Huo JH, and Wang ZQ
- Subjects
- Acute Kidney Injury mortality, Acute Kidney Injury prevention & control, Cardiac Surgical Procedures mortality, Cardiovascular Agents adverse effects, Drug Administration Schedule, Heart Diseases diagnosis, Heart Diseases mortality, Heart Diseases physiopathology, Humans, Incidence, Intensive Care Units, Length of Stay, Perioperative Care adverse effects, Renal Replacement Therapy, Respiration, Artificial, Risk Factors, Simendan adverse effects, Stroke Volume, Time Factors, Treatment Outcome, Ventricular Function, Left, Cardiac Surgical Procedures adverse effects, Cardiovascular Agents administration & dosage, Heart Diseases surgery, Perioperative Care methods, Simendan administration & dosage
- Abstract
Severe postoperative complications can affect cardiac surgery patients. Levosimendan is a novel calcium sensitizer commonly administered after cardiac surgery. However, the patient benefits are controversial. PubMed, Embase, and the Cochrane library were systematically searched for randomized controlled trials comparing levosimendan with control in adult cardiac surgery patients. Twenty-five studies (3247 patients) were included. Pooled data indicated that levosimendan reduced mortality after cardiac surgery [odds ratio (OR) 0.63, 95% confidence interval (CI): 0.47-0.84, P = 0.001]. However, this reduction was restricted to patients with low (<50%) left ventricular ejection fraction (OR 0.49, 95% CI: 0.35-0.70, P = 0.0001). It significantly reduced the incidence of postoperative acute kidney injury (OR 0.55, 95% CI: 0.41-0.74, P < 0.0001) and renal replacement therapy use (OR 0.56, 95% CI: 0.39-0.80, P = 0.002). Moreover, levosimendan significantly shortened the duration of the intensive care unit stay (weighted mean differences -0.49 day, 95% CI: -0.75 to -0.24, P = 0.0002) and mechanical ventilation use (weighted mean differences -2.30 hours, 95% CI: -3.76 to -0.84, P = 0.002). In conclusion, levosimendan reduced the mortality in patients with low left ventricular ejection fraction and decreased the incidence of acute renal injury and renal replacement therapy use. In addition, it shortened the duration of the intensive care unit stay and mechanical ventilation use.
- Published
- 2018
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39. Identification and characterization of major constituents in Juglans mandshurica using ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS).
- Author
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Huo JH, DU XW, Sun GD, Dong WT, and Wang WM
- Subjects
- Databases, Factual, Diarylheptanoids chemistry, Drugs, Chinese Herbal chemistry, Flavonoids chemistry, Hydroxybenzoates chemistry, Molecular Structure, Naphthoquinones chemistry, Reproducibility of Results, Triterpenes chemistry, Chromatography, High Pressure Liquid, Fruit chemistry, Juglans chemistry, Tandem Mass Spectrometry
- Abstract
The present study was designed to identify and characterize the major constituents in Juglans mandshurica Maxim. A simple, efficient and sensitive ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS) method was established and validated under positive and negative ion modes. The separation was performed on a Waters ACQUITY UPLC BEH C
18 column (2.1 mm × 100 mm, 1.7 μm) by gradient elution with a mobile phase (Phase A: 0.1% aqueous formic acid solution, Phase B: 0.1% formic acid acetonitrile solution). A total of 165 compounds were rapidly selected by Targeted and Non-Targeted Peak Finding approaches, and then tentatively identifled by comparing with reference substances or inferred through mass spectrometry fragment ion analysis and literature data. These compounds included 68 naphthalenequinones, 20 diarylheptanoids, 29 flavonoids, 20 triterpenes, and 28 phenolic acids. In conclusion, the present study provided an effective approach to identifying components in complex matrices of herbal medicines such as Juglans mandshurica Maxim., (Copyright © 2018 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.)- Published
- 2018
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40. miRNA-150-5p associate with antihypertensive effect of epigallocatechin-3-gallate revealed by aorta miRNome analysis of spontaneously hypertensive rat.
- Author
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Qian BJ, Tian CC, Ling XH, Yu LL, Ding FY, Huo JH, Zhu LC, Wen YL, Zhang JH, and Jing P
- Subjects
- Animals, Aorta drug effects, Biomarkers metabolism, Blood Pressure drug effects, Catechin pharmacology, Gene Expression Profiling, Hypertension drug therapy, Hypertension pathology, Male, Rats, Rats, Inbred SHR, Rats, Sprague-Dawley, Antihypertensive Agents pharmacology, Aorta metabolism, Catechin analogs & derivatives, Gene Expression Regulation drug effects, Hypertension genetics, MicroRNAs genetics, Tea chemistry
- Abstract
Aims: The antihypertensive mechanism (s) of the epigallocatechin-3-gallate (EGCG), a major effective component in green tea, might associate with microRNAs (miRNAs). Here, we aimed to investigate which microRNA in aorta of spontaneously hypertensive rats (SHRs) were modulated by administration of EGCG and its mechanism., Main Methods: The pharmacokinetic behaviors of EGCG and epigallocatechin (EGC) in Sprague-Dawley rats were analyzed by HPLC and DRUG AND STATISTICS software. Blood pressure of SHRs was monitored by the tail-cuff method, the miRNomes of aorta from SHRs was analyzed with deep sequencing, and expression of hypertension-associated miRNAs with significant change and their host genes and target genes were validated by real-time PCR and Western blot., Key Findings: The plasma deposition of EGCG and EGC best fitted a mono-compartmental model with maximum plasma concentration post-dose (C
max , 6.65 vs 4.45 μg/ml) and the corresponding time (Tmax , 15 vs 10 min). Systolic blood pressure (SBP) of SHRs decreased to the lowest point by 34.04 mmHg and recovered by 23.39 mmHg after 15 and 30 min of administration at dose of 300 mg/kg BW EGCG, respectively, and it decreased again at 60 min and recovered at time 2 h. Total 35 upregulated and 18 downregulated miRNAs were identified compared to the control group (p < .01) after EGCG administration. Expression of hypertension-associated miRNA-126a-3p and miRNA-150-5p were further validated. In turn, their host gene and target genes were up-regulated and down-regulated, respectively., Significance: Our results indicated that miRNA-150-5p might be involved in the antihypertensive effect of EGCG through SP1/AT1R pathway., (Copyright © 2018 Elsevier Inc. All rights reserved.)- Published
- 2018
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41. [Advances in application of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 system in stem cells research].
- Author
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Sun SJ, Huo JH, Geng ZJ, Sun XY, and Fu XB
- Subjects
- Animals, Humans, Stem Cells, CRISPR-Cas Systems, Clustered Regularly Interspaced Short Palindromic Repeats, Gene Editing methods
- Abstract
Gene engineering has attracted worldwide attention because of its ability of precise location of disease mutations in genome. As a new gene editing technology, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system is simple, fast, and accurate to operate at a specific gene site. It overcomes the long-standing problem of conventional operation. At the same time, stem cells are a good foundation for establishing disease model in vitro. Therefore, it has great significance to combine stem cells with the rapidly developing gene manipulation techniques. In this review, we mainly focus on the mechanism of CRISPR/Cas9 technology and its application in stem cell genomic editing, so as to pave the way for promoting rapid application and development of CRISPR/Cas9 technology.
- Published
- 2018
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42. [Fitness of sexual reproduction of Toona ciliata var. pubescens natural populations and their sexual reproduction and regeneration].
- Author
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Huang HL, Zhang L, Jia LM, Liang YL, and Cai JH
- Subjects
- Breeding, Genetic Variation, Pollination, Seedlings, Seeds, Trees, Genetic Fitness, Meliaceae, Reproduction
- Abstract
To examine the reproduction fitness coefficients and individual-level fitness of Toona ciliata var. pubescens, their sexual reproduction and natural regeneration were investigated during 2006-2016, with four natural populations in Jiulianshan National Reserve as test objects. The results showed that there were only 2-10 trees for the natural populations of T. ciliata var. pubescens with a small initial number of fruiting plants (3-9 trees), which were from the initial fruiting plants or their first/second generation. The sexual reproduction of these isolated populations were significantly different, and their seed production capacities tended to decline over time. With the maturing of communities, soil seed banks and seed germinations were extremely poor, and the number of trees that could be growing to mature stage was nearly zero. The optimum maturity age of T. ciliata var. pubescens was about 40 a, and the fitness coefficients (2.0-2.8) rapidly increased in early development stage, but then was sharply reduced (0.3-0.5), and then gradually dropped to almost 0. There were significant differences in the fitness at individual level (0-14 tree·cm
-2 ) among different populations, but their values were low (close to zero). Based on the existing reproduction rate, the actual values of sexual reproduction and regeneration fitness were much lower than the predicted ones. Due to the low level of genetic fitness, the sexual reproductive ability of different populations all showed decreasing trends. The natural sexual regeneration ability tended to decline, while the fitness of T. ciliata var. pubescens further decreased. All those factors suggested higher investment risks. Therefore, the systems of sexual reproduction became unbalanced and deteriorating. We proposed that more studies, including breeding mating, pollination, seed setting, and genetic diversity evaluation, are needed. Moreover, we should provide suitable forest environment through cleaning up litter in the fruiting stage and applying appropriate thinning during the transition period from seedling to young tree growth.- Published
- 2018
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43. Research on Controllable Degradation of Novel Sulfonylurea Herbicides in Acidic and Alkaline Soils.
- Author
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Zhou S, Hua XW, Wei W, Gu YC, Liu XQ, Chen JH, Chen MG, Xie YT, Zhou S, Meng XD, Zhang Y, Li YH, Wang BL, Song HB, and Li ZM
- Subjects
- China, Kinetics, Soil chemistry, Herbicides chemistry, Soil Pollutants chemistry, Sulfonylurea Compounds chemistry
- Abstract
The degradation issue of sulfonylurea (SU) has become one of the biggest challenges that hamper the development and application of this class of herbicides, especially in the alkaline soils of northern China. On the basis of the previous discovery that some substituents on the fifth position of the benzene ring in Chlorsulfuron could hasten its degradation rate, apparently in acidic soil, this work on Metsulfuron-methyl showed more convincing results. Two novel compounds (I-1 and I-2) were designed and synthesized, and they still retained potent herbicidal activity in tests against both dicotyledons and monocotyledons. The half-lives of degradation (DT
50 ) assay revealed that I-1 showed an accelerated degradation rate in acidic soil (pH 5.59). Moreover, we delighted to find that the degradation rate of I-1 was 9-10-fold faster than that of Metsulfuron-methyl and Chlorsulfuron when in alkaline soil (pH 8.46), which has more practical value. This research suggests that a modified structure that has potent herbicidal activity as well as accelerated degradation rate could be realized and this approach may provide a way to improve the residue problem of SUs in farmlands with alkaline soil.- Published
- 2017
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44. [Dynamic variation of components in exocarp of Juglans mandshurica with browning based on UPLC-Q-TOF/MS].
- Author
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Sun GD, Huo JH, Xie RJ, and Wang WM
- Subjects
- Chromatography, High Pressure Liquid, Drugs, Chinese Herbal chemistry, Flavones analysis, Glycosides analysis, Hydroxybenzoates analysis, Naphthoquinones analysis, Oxidation-Reduction, Tandem Mass Spectrometry, Fruit chemistry, Juglans chemistry
- Abstract
To analyze the dynamic changes in components in exocarp of Juglans mandshurica at different browning periods. Twenty-six batches of exocarp of J. mandshurica samples from thirteen browning periods were assessed by UPLC-Q-TOF-MS/MS. The formula of different compounds were determined by accurate mass and isotopic abundance ratio from target screening function of Peakview 2.0/masterview1.0 software. Then their structures were determined by analysis of MS/MS fragment or comparison with standard substances and references. The contents of chemical components were changed significantly in different browning periods and twenty five compounds were identified or inferred. Of the 13 naphthoquinone compounds, the contents of 6 compounds with similar parent nucleus as juglone and 3 naphthoquinone glycosides compounds were decreased significantly, and 4 naphthoquinone derivatives such as regiolone were produced; the contents of four flavones and two phenolic acids compounds were decreased significantly; and the contents of 6 diarylheptanoids compounds were increased significantly. UPLC-Q-TOF/MS method can be used to identify and analyze the chemical constituents from exocarp of J. mandshurica rapidly and accurately, and analyze the rules of dynamic changes, to reveal the browning of Chinese medicinal materials and its effects on compositions of fruits and vegetables., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2017
- Full Text
- View/download PDF
45. The electrochemical discrimination of pinene enantiomers by a cyclodextrin metal-organic framework.
- Author
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Deng CH, Li T, Chen JH, Ma JG, and Cheng P
- Subjects
- Bicyclic Monoterpenes, Electrochemical Techniques, Electrodes, Oxidation-Reduction, Stereoisomerism, Bridged Bicyclo Compounds chemistry, Cyclodextrins chemistry, Metal-Organic Frameworks chemistry, Monoterpenes chemistry
- Abstract
Pinene is a family of bicyclic monoterpenes found in nature, which exhibits important applications in chemical industry and biomedicine; however, the discrimination methods used for pinene enantiomers are still rare. The alpha- and beta-pinene enantiomers were recognized and discriminated via an electrochemical method for the first time based on a cyclodextrin metal-organic framework (CD-MOF) as an electrochemical chiral sensor.
- Published
- 2017
- Full Text
- View/download PDF
46. [Nasal resorption of prim-O-glucosylcimifugin and 5-O-methylvisammioside in rats].
- Author
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Wang TF, Wang JP, Pan JH, Jiang ZT, Chen XF, and Wang JC
- Subjects
- Administration, Intranasal, Animals, Chromatography, High Pressure Liquid, Drugs, Chinese Herbal pharmacokinetics, Rats, Drugs, Chinese Herbal administration & dosage, Monosaccharides pharmacokinetics, Nasal Mucosa metabolism, Xanthenes pharmacokinetics
- Abstract
In this experiment, rat nasal mucosa absorption characteristics of prim-O-glucosylcimifugin and 5-O-methylvisammioside were studied to provide a basis for drug delivery of Toutongning nasal spray. The nasal mucosa absorption test in rats was conducted with in situ nasal perfusion method after pH 6 buffer solution was used to prepare high, medium and low concentrations of prim-O-glucosylcimifugin, 5-O-methylvisammioside mixed solution as liquid circulation in nasal cavity. Then the concentrations of the circulating liquid compositions to be measured were determined by HPLC, and the absorption rates of prim-O-glucosylcimifugin and 5-O-methylvisammioside under different pH conditions were also investigated. According to the results, the absorption rate constant was (0.588±0.041)×10⁻³, (0.547±0.023)×10⁻³, (0.592±0.063)×10⁻³ min⁻¹ for prim-O-glucosylcimifugin high, middle and low concentrations, and (0.438±0.041)×10⁻³, (0.407±0.023)×10⁻³, and (0.412±0.063)×10⁻³ min⁻¹ for 5-O-methylvisammioside high, middle and low concentrations. There was no significant difference among high, middle and low concentration groups, and the absorption under pH 6 was better than that under other pH conditions. Therefore, we can get the conclusion that the main active ingredient of Toutongning nasal sprays can be absorbed through the nasal mucosa, and it is feasible to make nasal spray; in addition, pH 6 of nasal spray is scientific and reasonable., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2017
- Full Text
- View/download PDF
47. An Adaptable Continuous Restricted Boltzmann Machine in VLSI for Fusing the Sensory Data of an Electronic Nose.
- Author
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Wang JH, Tang CT, and Chen H
- Abstract
An embedded system capable of fusing sensory data is demanded for many portable or implantable microsystems. The continuous restricted Boltzmann machine (CRBM) is a probabilistic neural network not only capable of classifying data reliably but also amenable to very-large-scale-integration (VLSI) implementation. Although the embedded system based on the CRBM has been demonstrated with analog VLSI, the precision required by the learning algorithm is hardly achievable with analog circuits. Therefore, this paper investigates the feasibility of realizing the CRBM as a digital embedded system for fusing the sensory data of an electronic nose (eNose). The fusion here refers to data clustering and dimensional reduction that facilitates reliable classification. The capability of the CRBM to model different types of eNose data is first examined by MATLAB simulation. Afterward, the CRBM algorithm is customdesigned as a digital embedded system within an eNose microsystem. The functionality of the embedded CRBM system is then tested and discussed. With on-chip learning ability, the CRBM-embedded eNose is able to adapt its parameters in response to new data inputs or environmental changes.
- Published
- 2017
- Full Text
- View/download PDF
48. [Serum metabonomics in mice infected with mycoplasma pneumoniae by UPLC-Q-TOF-MS].
- Author
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Wei WF, Chu YT, Liu Y, Huo JH, and Wang WM
- Subjects
- Animals, Biomarkers blood, Chromatography, High Pressure Liquid, Mice, Mycoplasma pneumoniae, Pneumonia, Mycoplasma blood, Tandem Mass Spectrometry, Metabolome, Metabolomics, Pneumonia, Mycoplasma metabolism
- Abstract
Ultra high performance liquid chromatography coupled with tandem quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS) was applied to metabonomics study in BALB/c mice infected with mycoplasma pneumoniae(MP) to analyze the changes in serum endogenous metabolites, identify potential biomarkers associated with mycoplasma pneumoniae pneumonia(MPP), analyze the metabolic pathway and explore the pathogenic mechanism of MPP. The BALB/c mice were inoculated with MP by repeated intranasal infectious routes to establish MPP models, and the results of the lung tissue biopsy, IgM and mycoplasma nucleic acid content determination showed that the models of MP in BALB/c mice were successfully established. UPLC-Q-TOF-MS was used to analyze the serum metabolic profiling of BALB/c mice infected with MP, and then principal component analysis(PCA) was combined with orthogonal partial least squares discriminant analysis(OPLS-DA) for data processing. The results showed that there were significant differences in serum metabolic profile between the MP infected mice and the normal mice. Forty-seven potential biomarkers such as ornithine, cortisol, vitamin A and tryptophan were screened out by database searching and MS information matching. These potential biomarkers related to 17 metabolic pathways including retinol metabolism, arginine and proline metabolism, steroid hormone synthesis and so on. The metabonomic research method for serum of mice infected with mycoplasma pneumoniae based on UPLC-Q-TOF-MS was established in this study. The metabolic changes of endogenous small molecules in mice infected with MP were reflected in the overall level, laying the foundation for the selection and evaluation of MPP drugs., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2017
- Full Text
- View/download PDF
49. [Serum pharmacochemistry of Qinbai Qingfei concentrated pellets based on UPLC-Q-TOF-MS].
- Author
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Liu Y, Wei WF, Huo JH, and Wang WM
- Subjects
- Animals, Chromatography, High Pressure Liquid, Rats, Tandem Mass Spectrometry, Drugs, Chinese Herbal pharmacology, Serum chemistry
- Abstract
To analyze the main components of Qinbai Qingfei concentrated pellets in rat serum with UPLC-Q-TOF-MS technology and serum pharmacochemistry theory. After gavage administration with Qinbai Qingfei concentrated pellets, blood was collected from hepatic portal vein. ACQUITY UPLC BEH C₁₈(2.1 mm×100 mm, 1.7 μm) was used, with 0.1% formic acid agueous solution(A)-0.1%formic acid and acetonitrile(B) as the mobile phase for gradient elution. The flow rate was 0.3 mL•min⁻¹, the column temperature was maintained at 35 ℃. Through the comparative analysis fingerprints of Qinbai Qingfei concentrated pellets, drug containing-serum and blank serum, and with the help Peakview and Metabolitepilot software, components in serum were defined. A total of 28 compounds were identified, including 18 prototypes and 10 metabolites. As a result, UPLC-Q-TOF-MS technology and serum pharmacochemistry theory were applied to comprehensively expound Qinbai Qingfei concentrated pellets'constituents migrating to rat serum, and provide scientific basis for further studies for in vivo metabolic process and effective material base., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2017
- Full Text
- View/download PDF
50. [Screen absorption enhancer for intranasal administration preparations of paeoniflorin based on nasal perfusion method in rats].
- Author
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Zhu ZT, Pan JH, Wu XH, and Jiang ZT
- Subjects
- Animals, Chromatography, High Pressure Liquid, Rats, Administration, Intranasal, Glucosides administration & dosage, Monoterpenes administration & dosage, Nasal Mucosa drug effects
- Abstract
This experiment was aimed to screen the absorption enhancer for intranasal administration preparations of paeoniflorin. In this study, HPLC method for determination of paeoniflorin in perfusion liquid was established and the improved model of nasal perfusion in rats was used to screen out the species and amounts of absorption enhancer. In order to avoid the influence of the secretion and absorption of nasal cavity on the volume of perfusion fluid, the residual dose was calculated by using the volume correction method. Linear regression was carried out between the logarithm to the percentage of the residual dose and the corresponding time, and the slope of the regression line was exactly the absorption rate constant. Experimental results showed that hydroxypropyl-β-cyclodextrin and water-soluble azone can significantly improve the nasal absorption of paeoniflorin. Furthermore, water-soluble azone had the highest absorption rate constant and the best promoting penetration effect on intranasal administration preparations of paeoniflorin. It was also found that when the mass concentration of water-soluble azone in the perfusion liquid increased from 5 g•L⁻¹ to 20 g•L⁻¹, the absorption rate constant was gradually increased and peaked at 20 g•L⁻¹. When the mass concentration was increased to 30 g•L⁻¹, the absorption rate constant was decreased, indicating that the best mass concentration of water-soluble azone was 20 g•L⁻¹., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2017
- Full Text
- View/download PDF
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