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Purpose: Post-concussive visually induced dizziness (VID), in which symptoms are provoked by exposure to complex visual motion, is associated with protracted recovery. Although vestibular rehabilitation therapy (VRT) is recommended to treat post-concussive dizziness, there is sparse literature reporting on specific VRT interventions treating VID., Methods: A consecutive series of 26 individuals referred for VRT post-concussion were retrospectively assessed for inclusion in this case series. Each participant underwent a combination of conventional VRT and a technology-enhanced visual desensitization home exercise program (HEP). Self-report and objective measures were recorded from initial and discharge therapy evaluations. Twenty-three individuals (mean age 23.1 ± 12.4) with post-concussive dizziness (mean 109 ± 56 days post-injury) and no evidence for peripheral vestibular dysfunction were included. Treatment duration averaged 6.9 ± 2.5 weeks., Results: There were significant improvements in post-intervention on subjective and objective measures of dizziness and gait ( p  < .05). The response to intervention was independent of pre-injury migraine history but pre-injury depression/anxiety increased self-report of post-treatment anxiety. Concurrent treatment with medications did not influence response to treatment., Conclusions: The combination therapy intervention improved outcome measures consistent with VRT treatment outcomes in both concussion and non-traumatic vestibular conditions. Individuals referred for VRT post-concussion warrant assessment for VID and may benefit from the addition of technology-enhanced visual desensitization.

Background and Purpose: Vestibular symptoms are common after concussion. Vestibular Ocular Motor Screening identifies vestibular impairment, including postconcussive visual motion sensitivity, though the underlying functional brain alterations are not defined. We hypothesized that alterations in multisensory processing are responsible for postconcussive visual motion sensitivity, are detectable on fMRI, and correlate with symptom severity., Materials and Methods: Twelve patients with subacute postconcussive visual motion sensitivity and 10 healthy control subjects underwent vestibular testing and a novel fMRI visual-vestibular paradigm including 30-second "neutral" or "provocative" videos. The presence of symptoms/intensity was rated immediately after each video. fMRI group-level analysis was performed for a "provocative-neutral" condition. Z -statistic images were nonparametrically thresholded using clusters determined by Z > 2.3 and a corrected cluster significance threshold of P = .05. Symptoms assessed on Vestibular Ocular Motor Screening were correlated with fMRI mean parameter estimates using Pearson correlation coefficients., Results: Subjects with postconcussive visual motion sensitivity had significantly more Vestibular Ocular Motor Screening abnormalities and increased symptoms while viewing provocative videos. While robust mean activation in the primary and secondary visual areas, the parietal lobe, parietoinsular vestibular cortex, and cingulate gyrus was seen in both groups, selective increased activation was seen in subjects with postconcussive visual motion sensitivity in the primary vestibular/adjacent cortex and inferior frontal gyrus, which are putative multisensory visual-vestibular processing centers. Moderate-to-strong correlations were found between Vestibular Ocular Motor Screening scores and fMRI activation in the left frontal eye field, left middle temporal visual area, and right posterior hippocampus., Conclusions: Increased fMRI brain activation in visual-vestibular multisensory processing regions is selectively seen in patients with postconcussive visual motion sensitivity and is correlated with Vestibular Ocular Motor Screening symptom severity, suggesting that increased visual input weighting into the vestibular network may underlie postconcussive visual motion sensitivity., (© 2021 by American Journal of Neuroradiology.)

Background and Aims: Data on colorectal EMR (C-EMR) training are lacking. We aimed to evaluate C-EMR training among advanced endoscopy fellows (AEFs) by using a standardized assessment tool (STAT)., Methods: This multicenter prospective study used a STAT to grade AEF training in C-EMR during their 12-month fellowship. Cumulative sum analysis was used to establish learning curves and competence for cognitive and technical components of C-EMR and overall performance. Sensitivity analysis was performed by varying failure rates. AEFs completed a self-assessment questionnaire to assess their comfort level with performing C-EMR at the completion of their fellowship., Results: Six AEFs (189 C-EMRs; mean per AEF, 31.5 ± 18.5) were included. Mean polyp size was 24.3 ± 12.6 mm, and mean procedure time was 22.6 ± 16.1 minutes. Learning curve analyses revealed that less than 50% of AEFs achieved competence for key cognitive and technical C-EMR endpoints. All 6 AEFs reported feeling comfortable performing C-EMR independently at the end of their training, although only 2 of them achieved competence in their overall performance. The minimum threshold to achieve competence in these 2 AEFs was 25 C-EMRs., Conclusions: A relatively low proportion of AEFs achieved competence on key cognitive and technical aspects of C-EMR during their 12-month fellowship. The relatively low number of C-EMRs performed by AEFs may be insufficient to achieve competence, in spite of their self-reported readiness for independent practice. These pilot data serve as an initial framework for competence threshold, and suggest the need for validated tools for formal C-EMR training assessment., (Copyright © 2021. Published by Elsevier Inc.)

Introduction/purpose: Bariatric surgery (BS) has emerged as a cornerstone procedure to prevent and treat obesity-related comorbidities. As the Hispanic population continues to grow in the USA, their importance to the healthcare system cannot be understated. We aimed to assess the use of BS and related healthcare outcomes in Hispanics using a national database., Materials and Methods: Case-control study using the 2010 to 2014 National Inpatient Sample datasets. BS use in Hispanics compared to non-Hispanics was the primary outcome. Secondary outcomes included inpatient mortality, morbidity, resource use, length of hospital stay, hospital costs, and total hospitalization charges. Propensity scores were used to match Hispanic patients with BS with non-Hispanic patients with BS using sex, age, and Charlson Comorbidity Index as covariates. A multivariate model was then used to adjust for additional confounding factors., Results: From the 105,435 patients who underwent BS, a propensity-matched cohort of 20,440 was created (10,945 Hispanics). Mean (SD) age was 45 (17.2) years, and 73,594 (69.8%) were women. The prevalence of BS in Hispanics was 21/100,000 persons (281/100,000 admissions) compared to 36/100,000 persons (337/100,000 admissions) for non-Hispanics. On multivariate analysis, Hispanics displayed adjusted propensity-matched odds of 0.88 of having BS (P < 0.01). No differences were seen in the surgical approach performed. Hispanics and non-Hispanics had similar mortality, morbidity, hospital length of stay, and costs., Conclusion: Despite higher obesity rates, the use of BS is lower in Hispanics. For those who underwent BS, no difference in clinical outcomes and minor differences in resource use were observed.

Malnutrition is prevalent in cirrhosis. Vitamin and mineral deficiencies, including vitamin D, vitamin A, and zinc, are common and have been shown to correlate with survival. Our aim was to review the mechanisms of vitamin D, vitamin A, and zinc deficiencies in cirrhosis and the clinical assessment of affected patients, their outcomes based on the current literature, and management. This is a narrative review including the relevant literature for cirrhosis and vitamin D, vitamin A, and zinc deficiencies. Vitamin D deficiency has important effects in cirrhosis, regardless of the cause of chronic liver disease.These effects include associations with fibrosis and outcomes such as infections, hepatocellular carcinoma, and mortality. Vitamin A deficiency is associated with liver disease progression to cirrhosis and clinical decompensation, including occurrence of ascites or hepatic encephalopathy. Zinc deficiency can lead to hepatic encephalopathy and impaired immune function. Such deficiencies correlate with patient survival and disease severity. Caution should be applied when replacing vitamin D, vitamin A, and zinc to avoid toxicity. Identification and appropriate treatment of vitamin and mineral deficiencies in cirrhosis may reduce specific nutritional and cirrhosis-related adverse events. Routine monitoring of vitamin A, vitamin D and zinc levels in cirrhosis should be considered.

Combinatorial drug treatment strategies perturb biological networks synergistically to achieve therapeutic effects and represent major opportunities to develop advanced treatments across a variety of human disease areas. However, the discovery of new combinatorial treatments is challenged by the sheer scale of combinatorial chemical space. Here, we report a high-throughput system for nanoliter-scale phenotypic screening that formulates a chemical library in nanoliter droplet emulsions and automates the construction of chemical combinations en masse using parallel droplet processing. We applied this system to predict synergy between more than 4,000 investigational and approved drugs and a panel of 10 antibiotics against Escherichia coli , a model gram-negative pathogen. We found a range of drugs not previously indicated for infectious disease that synergize with antibiotics. Our validated hits include drugs that synergize with the antibiotics vancomycin, erythromycin, and novobiocin, which are used against gram-positive bacteria but are not effective by themselves to resolve gram-negative infections., Competing Interests: Conflict of interest statement: Broad Institute and MIT may seek to commercialize aspects of this work; related applications for intellectual property have been filed.

mRNA therapeutics hold promise for the treatment of diseases requiring intracellular protein expression and for use in genome editing systems, but mRNA must transfect the desired tissue and cell type to be efficacious. Nanoparticle vectors that deliver the mRNA are often evaluated using mRNA encoding for reporter genes such as firefly luciferase (FLuc); however, single-cell resolution of mRNA expression cannot generally be achieved with FLuc, and, thus, the transfected cell populations cannot be determined without additional steps or experiments. To more rapidly identify which types of cells an mRNA formulation transfects in vivo, we describe a Cre recombinase (Cre)-based system that permanently expresses fluorescent tdTomato protein in transfected cells of genetically modified mice. Following in vivo application of vectored Cre mRNA, it is possible to visualize successfully transfected cells via Cre-mediated tdTomato expression in bulk tissues and with single-cell resolution. Using this system, we identify previously unknown transfected cell types of an existing mRNA delivery vehicle in vivo and also develop a new mRNA formulation capable of transfecting lung endothelial cells. Importantly, the same formulations with mRNA encoding for fluorescent protein delivered to wild-type mice did not produce sufficient signal for any visualization in vivo, demonstrating the significantly improved sensitivity of our Cre-based system. We believe that the system described here may facilitate the identification and characterization of mRNA delivery vectors to new tissues and cell types., (Copyright © 2017 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights reserved.)

Objective: The Ages and Stages Questionnaires Edition 3 (ASQ-3) are a well-validated international screen for developmental delays in young children. However, previous studies demonstrate variable scores between children eligible to take the same ASQ-3 interval. This study aimed to determine a relationship between age and ASQ-3 score for each screening interval., Methods: This was a baseline exploratory cross-sectional study of infants under 2 years old evaluated for the Peruvian social programme Cuna Más. Participants were included in Cuna Más if they lived in districts with fewer than 2000 inhabitants or 400 homes, indicating a predominantly rural population. The appropriate ASQ-3 screening interval was administered to each subject. Subjects were divided into four 2-week chronological subgroups based on age within each 2-month screening window and aggregated across all 12 screening intervals. Fisher's exact test, analysis of variance and Bonferonni post hoc test were used to compare differences between age subgroups. Linear regression was performed to assess the relationship between ASQ-3 score and both aggregated and disaggregated age subgroup., Results: A total of 5850 Peruvian infants were evaluated in 2013. Mean age was 13 ± 6.6 months, 50.7% were male and mean maternal education was 6.6 ± 4.0 years; 34.8% infants were stunted, 7.8% were underweight, 0.9% were wasted and 2% had age adjusted greater than 35 days for prematurity for ASQ-3 interval assignment. Mean total ASQ-3 was 42.2 ± 8.2. The ASQ-3 allocated 49.6% with suspected delay in one or more developmental areas. Before and after adjusting for wealth quintile, maternal education level, infant nutritional status and prematurity adjustment, age subgroup remained significantly associated with total ASQ-3 score (β = 1.8, CI: 1.7-2.0, P < 0.001), sectional ASQ-3 score (all P < 0.001) and inversely associated with one or more scores indicating suspected developmental delay (P < 0.001)., Conclusions: The ASQ-3 may underestimate the sensitivity of child development to small differences in age in this population., (© 2017 John Wiley & Sons Ltd.)

Objectives: To understand the social representations of the Awajún and Wampis communities related to the symptoms and treatment of anemia in children younger than 3 years, as well as the relationship of these representations with the symbolism, constructs, and type of diet of these populations., Materials and Methods: This qualitative study was conducted from June to August 2015 in the districts of Río Santiago, Cenepa, and Nieva (Amazon region, Peru), and included in-depth interviews (IDIs) of health personnel, community authorities, parents, and focus groups (FGs) for mothers., Results: A total of 38 IDIs and 13 FGs were conducted. The evaluated populations had limited awareness about anemia and health personnel, but anemia with symptoms were correlated with social representations and cultural manifestations. This behavior was reflected in the parents' choice of treatments that were not necessarily the same as those indicated by the health personnel, and these social groups preferred the consumption of certain foods that were considered to cure the "putsumat". Visiting a health care center or using micronutrients was not their first treatment option., Conclusions: Social representations and traditional practices still exist, and include interpretative systems in health, disease, and disease management. The logic, meaning, and coherence of these practices depend on the cultural group considered. The "putsumat" or "putsuju" is an interpretive model for anemia, and the symptoms in children include pallor, thinness, and fatigue; this model is based on the cultural system of the Awajún and Wampis populations.

mRNA has broad potential for treating diseases requiring protein expression. However, mRNA can also induce an immune response with associated toxicity. Replacement of uridine bases with pseudouridine has been postulated to modulate both mRNA immunogenicity and potency. Here, we explore the immune response and activity of lipid nanoparticle-formulated unmodified and pseudouridine-modified mRNAs administered systemically in vivo. Pseudouridine modification to mRNA had no significant effect on lipid nanoparticle physical properties, protein expression in vivo, or mRNA immunogenicity compared to unmodified mRNA when delivered systemically with liver-targeting lipid nanoparticles, but reduced in vitro transfection levels. Indicators of a transient, extracellular innate immune response to mRNA were observed, including neutrophilia, myeloid cell activation, and up-regulation of four serum cytokines. This study provides insight into the immune responses to mRNA lipid nanoparticles, and suggests that pseudouridine modifications may be unnecessary for therapeutic application of mRNA in the liver., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

Introduction: Despite the reduction of poverty in Perú, the prevalence of anemia in the country remains high. , Objective: To identify socio-demographic, child and maternal-child care factors associated with anemia in children between 6 and 35 months in Perú. , Materials and Methods: We conducted an analytical and descriptive study that included registered data from the national survey on demography and family health, 2007-2013, on children between 6 and 35 months old, including the measurement of blood hemoglobin. Anemia was confirmed by hemoglobin-altitude corrected values below 11 mg/dl. We used multivariate logistic regression models to assess potential associated factors for anemia. , Results: Anemia prevalence was high (47.9%). Twelve factors were independently associated with anemia in children: Socio-demographic factors such as living outside Lima and Callao, in a low socioeconomic household, and having an adolescent mother with low education level; child-related factors as being male, younger than 24 months of age, and having fever in the previous two weeks, and maternal-child care factors such as lack of prenatal control in the first trimester of pregnancy, lack or short period of iron supplementation during pregnancy, house delivery, anemia detection at the moment of the survey, and lack of intestinal anti-parasite preventive treatment in the child. , Conclusions: The analysis of survey data provided valuable information about factors associated with anemia in children between 6 and 35 months, which can be used to increase the coverage and effectiveness of maternal-child care practices.

Objectives: To assess five elements of healthcare quality (physical space, equipment, personnel, waiting time, and counseling) in growth and development services (GDS) at 18 healthcare establishments in nine regions of Peru with high rates of poverty., Materials and Methods: A quali-quantitative study was carried out in the Amazonas, Apurímac, Ayacucho, Cajamarca, Cusco, Huánuco, Huancavelica, Puno, and Ucayali regions, that included direct observation (DO) during medical assistance in GDS, focus groups (FG, one user per healthcare establishment), and in-depth interviews (IDI) with healthcare workers (two per healthcare establishment)., Results: There were 18 DOs carried out to assess environment and equipment, 23 DOs of complete service shifts, 67 accompaniments to users during medical assistance in GDS, 18 FGs (118 participating mothers), and 36 IDIs. Both mothers and healthcare professionals agreed that there were limitations in infrastructure, equipment, materials, and personnel that impacted quality medical assistance in GDS. Delays in medical assistance, failure to comply with schedules, and lack of friendliness when providing medical care were the aspects that produced the greatest user dissatisfaction. Only 24.3% of the total amount of time spent at the healthcare establishment corresponded to beneficial activities for the user, although the Health Department was meeting the objective to promote improvement of childcare practices., Conclusions: The study was useful in identifying weaknesses and strengths that may help redirect GDS efforts within the framework of policies aimed at strengthening first-class medical assistance.

Objectives: To estimate the rate of neonatal mortality and to describe neonatal deaths in 2011 in hospitals of the Ministry of Health at Huanuco and Ucayali (Peru)., Materials and Methods: Cross-sectional study from September to November 2012 in Huanuco and Ucayali. Records of neonatal deaths in 2011 were reviewed from provincial municipalities, regional health directorates and four referral hospitals. To calculate mortality rates, we used the most reliable information sources by region. Reviewing 185 medical records in hospitals allowed us to describe the root causes of neonatal deaths., Results: In 2011, 10,886 live births and 158 neonatal deaths were reported in Huanuco, with a rate of 14.5 deaths per 1000 live births. In Ucayali, 11,441 live births and 138 neonatal deaths were reported, with a rate of 12.1 deaths per 1000 live births. Most hospital neonatal deaths occurred in the first 7 days of life (87%), in preterm infants (73.9%) and with low birth weight (67%). The most common underlying causes of neonatal deaths were infection (31.4%), congenital malformation (22.2%) and prematurity (18.9%)., Conclusions: Neonatal mortality rate in the studied regions was higher than the national average. The results suggest the need for effective and comprehensive interventions during pregnancy, childbirth and the early postnatal period; this last period is the most vulnerable in the neonate.

Objectives: To evaluate maternal and child care practices in areas with extreme poverty in Peru., Materials and Methods: Cross-sectional study conducted between August and September 2012; with probabilistic, three-stage stratification by "department" (geographic region) sampling. 540 households were selected that had at least one child younger than 36 months (475 households) and/or a pregnant women (80 households), in rural areas of Cajamarca, Amazonas, Huanuco, Ayacucho, Huancavelica, Apurimac, Cusco, Puno and Ucayali., Results: Regarding the last pregnancy, 69.0% of the mothers reported having had their first prenatal care in the first trimester; 65.3% reported having completed more than six check-ups throughout the pregnancy; 81.1% reported having given birth in a health facility, and only 31.0% chose a method of family planning within 42 days postpartum. With regard to the last child under 3 years old, 64.1% had early mother-infant contact, and 62.8% initiated breastfeeding within one hour of birth. In addition, 89.6% of children under 6 months old exclusively received breastfeeding and 89.1% of children aged 6-8 months old already had received complementary feeding. Fever, diarrhea, vomiting and breathing difficulty were the most mentioned warning signs for seeking care for children., Conclusions: Through this study, a baseline has been established on which a strategy can be designed and implemented to improve best practices for maternal and child care as part of the "Programa de Apoyo" within the Health Sector Reform.

Tuberculosis (TB) persists as a major public health problem in our country. The appearance of resistant strains has complicated its control and questioned the appropriateness of the current measures towards prevention and control. An analysis from social determinants related to TB, converge on irregular treatment that generates disease persistence and appearance of resistance to TB drugs. The objective of this paper is to identify the role of the government in the treatment of TB patients, to recognize difficulties of treatment adherence considering that its fulfillment depends on the patient, despite that it has direct consequences on public health, and to discuss TB management alternatives with an approach based on individual and collective human rights. International literature shows limit experiences of individual rights and collective ones, but based on health policies and health legislation. In Peru, a new approach is required to guarantee population health without infringing individual rights.