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Your search keyword '"Hutchinson, Chelsea M"' showing total 6 results
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6 results on '"Hutchinson, Chelsea M"'

1. Endurance Training Reprograms The White Adipose Tissue Proteome Of Rats In A Sex-specific Manner

Author

Many, Gina M., primary, Sanford, James A., additional, Piehowski, Paul D., additional, Petyuk, Vlad A., additional, Gritsenko, Marina A., additional, Hansen, Joshua R., additional, Hutchinson, Chelsea M., additional, Taylor, Zane, additional, Moore, Ronald J., additional, Nestor, Michael, additional, Qian, Wei-Jun, additional, and Adkins, Joshua N., additional

Published
2021
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2. Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates

Author

Chroumpi, Tania, Peng, Mao, Markillie, Lye Meng, Mitchell, Hugh D, Nicora, Carrie D, Hutchinson, Chelsea M, Paurus, Vanessa, Tolic, Nikola, Clendinen, Chaevien S, Orr, Galya, Baker, Scott E, Mäkelä, Miia R, de Vries, Ronald P, Molecular Plant Physiology, Sub Molecular Plant Physiology, Molecular Plant Physiology, Sub Molecular Plant Physiology, Westerdijk Fungal Biodiversity Institute - Fungal Physiology, Westerdijk Fungal Biodiversity Institute, Department of Microbiology, Fungal Genetics and Biotechnology, and Helsinki Institute of Sustainability Science (HELSUS)

Subjects
0301 basic medicine, Histology, lcsh:Biotechnology, 030106 microbiology, Biomedical Engineering, Pentose, Biomass, Bioengineering, Polysaccharide, 03 medical and health sciences, chemistry.chemical_compound, lcsh:TP248.13-248.65, sugar beet pulp, Monosaccharide, L-rhamnose catabolic pathway, Sugar, pentose catabolic pathway, Original Research, 2. Zero hunger, chemistry.chemical_classification, biology, lignocellulosic substrates, Aspergillus niger, Bioengineering and Biotechnology, food and beverages, 15. Life on land, biology.organism_classification, 220 Industrial biotechnology, Xyloglucan, 030104 developmental biology, chemistry, Biochemistry, D-galacturonic acid catabolic pathway, Sugar beet, wheat bran, CAZymes, Biotechnology
Abstract

The filamentous ascomycete Aspergillus niger has received increasing interest as a cell factory, being able to efficiently degrade plant cell wall polysaccharides as well as having an extensive metabolism to convert the released monosaccharides into value added compounds. The pentoses D-xylose and L-arabinose are the most abundant monosaccharides in plant biomass after the hexose D-glucose, being major constituents of xylan, pectin and xyloglucan. In this study, the influence of selected pentose catabolic pathway (PCP) deletion strains on growth on plant biomass and re-routing of sugar catabolism was addressed to gain a better understanding of the flexibility of this fungus in using plant biomass-derived monomers. The transcriptome, metabolome and proteome response of three PCP mutant strains, ΔlarAΔxyrAΔxyrB, ΔladAΔxdhAΔsdhA and ΔxkiA, grown on wheat bran (WB) and sugar beet pulp (SBP), was evaluated. Our results showed that despite the absolute impact of these PCP mutations on pure pentose sugars, they are not as critical for growth of A. niger on more complex biomass substrates, such as WB and SBP. However, significant phenotypic variation was observed between the two biomass substrates, but also between the different PCP mutants. This shows that the high sugar heterogeneity of these substrates in combination with the high complexity and adaptability of the fungal sugar metabolism allow for activation of alternative strategies to support growth.

Published
2021

3. Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates

Author

Molecular Plant Physiology, Sub Molecular Plant Physiology, Chroumpi, Tania, Peng, Mao, Markillie, Lye Meng, Mitchell, Hugh D, Nicora, Carrie D, Hutchinson, Chelsea M, Paurus, Vanessa, Tolic, Nikola, Clendinen, Chaevien S, Orr, Galya, Baker, Scott E, Mäkelä, Miia R, de Vries, Ronald P, Molecular Plant Physiology, Sub Molecular Plant Physiology, Chroumpi, Tania, Peng, Mao, Markillie, Lye Meng, Mitchell, Hugh D, Nicora, Carrie D, Hutchinson, Chelsea M, Paurus, Vanessa, Tolic, Nikola, Clendinen, Chaevien S, Orr, Galya, Baker, Scott E, Mäkelä, Miia R, and de Vries, Ronald P

Published
2021

6. Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates.

Author

Chroumpi T, Peng M, Markillie LM, Mitchell HD, Nicora CD, Hutchinson CM, Paurus V, Tolic N, Clendinen CS, Orr G, Baker SE, Mäkelä MR, and de Vries RP

Abstract

The filamentous ascomycete Aspergillus niger has received increasing interest as a cell factory, being able to efficiently degrade plant cell wall polysaccharides as well as having an extensive metabolism to convert the released monosaccharides into value added compounds. The pentoses D-xylose and L-arabinose are the most abundant monosaccharides in plant biomass after the hexose D-glucose, being major constituents of xylan, pectin and xyloglucan. In this study, the influence of selected pentose catabolic pathway (PCP) deletion strains on growth on plant biomass and re-routing of sugar catabolism was addressed to gain a better understanding of the flexibility of this fungus in using plant biomass-derived monomers. The transcriptome, metabolome and proteome response of three PCP mutant strains, Δ larA Δ xyrA Δ xyrB , Δ ladA Δ xdhA Δ sdhA and Δ xkiA , grown on wheat bran (WB) and sugar beet pulp (SBP), was evaluated. Our results showed that despite the absolute impact of these PCP mutations on pure pentose sugars, they are not as critical for growth of A. niger on more complex biomass substrates, such as WB and SBP. However, significant phenotypic variation was observed between the two biomass substrates, but also between the different PCP mutants. This shows that the high sugar heterogeneity of these substrates in combination with the high complexity and adaptability of the fungal sugar metabolism allow for activation of alternative strategies to support growth., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Chroumpi, Peng, Markillie, Mitchell, Nicora, Hutchinson, Paurus, Tolic, Clendinen, Orr, Baker, Mäkelä and de Vries.)

Published
2021
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