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13 results on '"Huvenne, Hanneke"'

2. RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design

Author

Terenius, Olle, Papanicolaou, Alexie, Garbutt, Jennie S., Eleftherianos, Ioannis, Huvenne, Hanneke, Kanginakudru, Sriramana, Albrechtsen, Merete, An, Chunju, Aymeric, Jean-Luc, Barthel, Andrea, Bebas, Piotr, Bitra, Kavita, Bravo, Alejandra, Chevalier, François, Collinge, Derek P., Crava, Cristina M., de Maagd, Ruud A., Duvic, Bernard, Erlandson, Martin, Faye, Ingrid, Felföldi, Gabriella, Fujiwara, Haruhiko, Futahashi, Ryo, Gandhe, Archana S., Gatehouse, Heather S., Gatehouse, Laurence N., Giebultowicz, Jadwiga M., Gómez, Isabel, Grimmelikhuijzen, Cornelis J.P., Groot, Astrid T., Hauser, Frank, Heckel, David G., Hegedus, Dwayne D., Hrycaj, Steven, Huang, Lihua, Hull, J. Joe, Iatrou, Kostas, Iga, Masatoshi, Kanost, Michael R., Kotwica, Joanna, Li, Changyou, Li, Jianghong, Liu, Jisheng, Lundmark, Magnus, Matsumoto, Shogo, Meyering-Vos, Martina, Millichap, Peter J., Monteiro, Antónia, Mrinal, Nirotpal, Niimi, Teruyuki, Nowara, Daniela, Ohnishi, Atsushi, Oostra, Vicencio, Ozaki, Katsuhisa, Papakonstantinou, Maria, Popadic, Aleksandar, Rajam, Manchikatla V., Saenko, Suzanne, Simpson, Robert M., Soberón, Mario, Strand, Michael R., Tomita, Shuichiro, Toprak, Umut, Wang, Ping, Wee, Choon Wei, Whyard, Steven, Zhang, Wenqing, Nagaraju, Javaregowda, ffrench-Constant, Richard H., Herrero, Salvador, Gordon, Karl, Swevers, Luc, and Smagghe, Guy

Published
2011
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9. Delivery of dsRNA for RNAi in insects: An overview and future directions

Author

Yu, Na, Christiaens, Olivier, Liu, Jisheng, Niu, Jinzhi, Cappelle, Kaat, Huvenne, Hanneke, Smagghe, Guy, CACCIA, SILVIA, Yu, Na, Christiaens, Olivier, Liu, Jisheng, Niu, Jinzhi, Cappelle, Kaat, Caccia, Silvia, Huvenne, Hanneke, and Smagghe, Guy

Subjects
Biochemistry, Genetics and Molecular Biology (all), Animal, Ingestion, Gene Transfer Technique, Ecology, Evolution, Behavior and Systematic, Microinjection, DsRNA uptake, Pest control, RNA interference, Insect Protein, Insect Science, Soaking, Insect, Agronomy and Crop Science, RNA, Double-Stranded
Published
2013

10. Search for limiting factors in the RNAi pathway in silkmoth tissues and the silkmoth-derived Bm5 cell line : the RNA-binding proteins R2D2 and Translin

Author

Swevers, Luc, Liu, Jisheng, Huvenne, Hanneke, and Smagghe, Guy

Subjects
EXPRESSION, GENOME, INTERFERENCE, DROSOPHILA, GENES, SILKWORM BOMBYX-MORI, fungi, INSECTS, Biology and Life Sciences, TRIBOLIUM-CASTANEUM, DOUBLE-STRANDED-RNA, NUCLEAR POLYHEDROSIS-VIRUS
Abstract

RNA interference (RNAi), an RNA-dependent gene silencing process that is initiated by double-stranded RNA (dsRNA) molecules, has been applied with variable success in lepidopteran insects, in contrast to the high efficiency achieved in the coleopteran Tribolium castaneum. To gain insight into the factors that determine the efficiency of RNAi, a survey was carried out to check the expression of factors that constitute the machinery of the small interfering RNA (siRNA) and microRNA (miRNA) pathways in different tissues and stages of the silkmoth, Bombyx mori. It was found that the dsRNA-binding protein R2D2, an essential component in the siRNA pathway in Drosophila, was expressed at minimal levels in silkmoth tissues. The silkmoth-derived Bm5 cell line was also deficient in expression of mRNA encoding full-length BmTranslin, an RNA-binding factor that has been shown to stimulate the efficiency of RNAi. However, despite the lack of expression of the RNA-binding proteins, silencing of a luciferase reporter gene was observed by co-transfection of luc dsRNA using a lipophilic reagent. In contrast, gene silencing was not detected when the cells were soaked in culture medium supplemented with dsRNA. The introduction of an expression construct for Tribolium R2D2 (TcR2D2) did not influence the potency of luc dsRNA to silence the luciferase reporter. Immunostaining experiments further showed that both TcR2D2 and BmTranslin accumulated at defined locations within the cytoplasm of transfected cells. Our results offer a first evaluation of the expression of the RNAi machinery in silkmoth tissues and Bm5 cells and provide evidence for a functional RNAi response to intracellular dsRNA in the absence of R2D2 and Translin. The failure of TcR2D2 to stimulate the intracellular RNAi pathway in Bombyx cells is discussed.

Published
2011

13. Delivery of dsRNA for RNAi in insects: an overview and future directions.

Author

Yu N, Christiaens O, Liu J, Niu J, Cappelle K, Caccia S, Huvenne H, and Smagghe G

Subjects
Animals, Insect Proteins genetics, Insect Proteins metabolism, Insecta metabolism, Gene Transfer Techniques trends, Insecta genetics, RNA, Double-Stranded genetics
Abstract

RNA interference (RNAi) refers to the process of exogenous double-stranded RNA (dsRNA) silencing the complementary endogenous messenger RNA. RNAi has been widely used in entomological research for functional genomics in a variety of insects and its potential for RNAi-based pest control has been increasingly emphasized mainly because of its high specificity. This review focuses on the approaches of introducing dsRNA into insect cells or insect bodies to induce effective RNAi. The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research. In this review, we also briefly introduce the two possible dsRNA uptake machineries, other dsRNA delivery methods and the history of RNAi in entomology. Factors that influence the specificity and efficiency of RNAi such as transfection reagents, selection of dsRNA region, length, and stability of dsRNA in RNAi research are discussed for further studies., (© 2012 The Authors Insect Science © 2012 Institute of Zoology, Chinese Academy of Sciences.)

Published
2013
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