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8 results on '"Huy-Hyen Oh"'

1. Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice

Author

Hye Lim Cha, Jung-Mi Choi, Huy-Hyen Oh, Narayan Bashyal, Sung-Soo Kim, Lutz Birnbaumer, and Haeyoung Suh-Kim

Subjects
Go alpha subunit (Gαo, GTP-binding protein alpha subunit of Go), Purkinje cell, Cerebellum, Hypoplasia, Synaptic boutons, Neurology. Diseases of the nervous system, RC346-429
Abstract

Abstract Go is a member of the pertussis toxin-sensitive Gi/o family. Despite its abundance in the central nervous system, the precise role of Go remains largely unknown compared to other G proteins. In the present study, we explored the functions of Go in the developing cerebellar cortex by deleting its gene, Gnao. We performed a histological analysis with cerebellar sections of adult mice by cresyl violet- and immunostaining. Global deletion of Gnao induced cerebellar hypoplasia, reduced arborization of Purkinje cell dendrites, and atrophied Purkinje cell dendritic spines and the terminal boutons of climbing fibers from the inferior olivary nucleus. These results indicate that Go-mediated signaling pathway regulates maturation of presynaptic parallel fibers from granule cells and climbing fibers during the cerebellar cortical development.

Published
2019
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2. Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice

Author

Jung-Mi Choi, Lutz Birnbaumer, Haeyoung Suh-Kim, Narayan Bashyal, Huy-Hyen Oh, Sung-Soo Kim, and Hye Lim Cha

Subjects
0301 basic medicine, Cerebellum, Go alpha subunit (Gαo, Dendritic spine, PURKINJE CELL, Purkinje cell, SYNAPTIC BOUTONS, GTP-Binding Protein alpha Subunits, Gi-Go, lcsh:RC346-429, GO ALPHA SUBUNIT (GΑO, purl.org/becyt/ford/1 [https], Purkinje Cells, chemistry.chemical_compound, 0302 clinical medicine, Heterotrimeric G protein, Inferior olivary nucleus, Synaptic boutons, CEREBELLAR DEVELOPMENT, Climbing fiber, Cerebral Cortex, Neurons, CEREBELO, PROTEINAS, Cerebellar development, HIPOPLASIA, humanities, 3. Good health, Cell biology, medicine.anatomical_structure, Cerebellar cortex, GTP-binding protein alpha subunit of Go), CIENCIAS NATURALES Y EXACTAS, GTP-BINDING PROTEIN ALPHA SUBUNIT OF GO), Presynaptic Terminals, Synaptic Membranes, Biology, Ciencias Biológicas, 03 medical and health sciences, Cellular and Molecular Neuroscience, Cresyl violet, Biología Celular, Microbiología, medicine, Animals, RNA, Messenger, purl.org/becyt/ford/1.6 [https], Molecular Biology, CEREBELLUM, lcsh:Neurology. Diseases of the nervous system, Research, SISTEMA NERVIOSO CENTRAL, Dendrites, Hypoplasia, Mice, Inbred C57BL, 030104 developmental biology, nervous system, chemistry, CLIMBING FIBER, HYPOPLASIA, Gene Deletion, 030217 neurology & neurosurgery
Abstract

Fil: Cha, Hye Lim. Ajou University School of Medicine. Departments of Anatomy; South Korea Fil: Choi, Jung-Mi. Ajou University School of Medicine. Departments of Anatomy; South Korea Fil: Oh, Huy-Hyen. Ajou University School of Medicine. Departments of Anatomy; South Korea Fil: Bashyal, Narayan. Ajou University School of Medicine. Departments of Anatomy; South Korea Fil: Bashyal, Narayan. Ajou University School of Medicine. The Graduate School. Departments of Biomedical Sciences; South Korea Fil: Kim, Sung-Soo. Ajou University School of Medicine. Departments of Anatomy; South Korea Fil: Birnbaumer, Lutz. Pontificia Univerisdad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina Fil: Birnbaumer, Lutz. Research Triangle Park. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados Unidos Fil: Suh-Kim, Haeyoung. Ajou University School of Medicine. Departments of Anatomy; South Korea Fil: Suh-Kim, Haeyoung. Ajou University School of Medicine. Departments of Anatomy; South Korea Abtract: Go is a member of the pertussis toxin-sensitive Gi/o family. Despite its abundance in the central nervous system, the precise role of Go remains largely unknown compared to other G proteins. In the present study, we explored the functions of Go in the developing cerebellar cortex by deleting its gene, Gnao. We performed a histological analysis with cerebellar sections of adult mice by cresyl violet- and immunostaining. Global deletion of Gnao induced cerebellar hypoplasia, reduced arborization of Purkinje cell dendrites, and atrophied Purkinje cell dendritic spines and the terminal boutons of climbing fibers from the inferior olivary nucleus. These results indicate that Go-mediated signaling pathway regulates maturation of presynaptic parallel fibers from granule cells and climbing fibers during the cerebellar cortical development.

Published
2019

7. Development of the main olfactory system and main olfactory epithelium-dependent male mating behavior are altered in Go-deficient mice

Author

Hye Lim Cha, Sung-Soo Kim, Chan-Il Choi, Young-Don Lee, Lutz Birnbaumer, Jung-Mi Choi, Huy-Hyen Oh, Haeyoung Suh-Kim, and Sungho Ghil

Subjects
0301 basic medicine, Olfactory system, Male, Cell type, Vomeronasal organ, Tyrosine 3-Monooxygenase, COMPORTAMIENTO, Neurogenesis, Sensory system, Apoptosis, Cell Count, Biology, GTP-Binding Protein alpha Subunits, Gi-Go, Models, Biological, 03 medical and health sciences, Mice, Sexual Behavior, Animal, 0302 clinical medicine, Olfactory Mucosa, SEXUALIDAD, Interneurons, medicine, Animals, RNA, Messenger, Cells, Cultured, Multidisciplinary, PROTEINAS, OLFATO, Biological Sciences, Olfactory Bulb, humanities, Olfactory bulb, APOPTOSIS, 030104 developmental biology, medicine.anatomical_structure, nervous system, Pheromone, Vomeronasal Organ, Neuroscience, Olfactory epithelium, 030217 neurology & neurosurgery, Gene Deletion, Signal Transduction
Abstract

Fil: Choi, Jung-Mi. Ajou University School of Medicine. Department of Anatomy; Corea del Sur Fil: Kim, Sung-Soo. Ajou University School of Medicine. Department of Anatomy; Corea del Sur Fil: Choi, Chan-Il. Ajou University School of Medicine. Department of Anatomy; Corea del Sur Fil: Cha, Hye Lim. Ajou University School of Medicine. Department of Anatomy; Corea del Sur Fil: Oh, Huy-Hyen. Ajou University School of Medicine. Department of Anatomy; Corea del Sur Fil: Oh, Huy-Hyen. Ajou University School of Medicine. The Graduate School. Department of Biomedical Sciences; Corea del Sur Fil: Ghil, Sungho. Kyonggi University. Department of Life Science; Corea del Sur Fil: Lee, Young-Don. Ajou University School of Medicine. Department of Anatomy; Corea del Sur Fil: Lee, Young-Don. Ajou University School of Medicine. The Graduate School. Department of Biomedical Sciences; Corea del Sur Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina Fil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados Unidos Fil: Suh-Kim, Haeyoung. Ajou University School of Medicine. Department of Anatomy; Corea del Sur Fil: Suh-Kim, Haeyoung. Ajou University School of Medicine. The Graduate School. Department of Biomedical Sciences; Corea del Sur Abstract: In mammals, initial detection of olfactory stimuli is mediated by sensory neurons in the main olfactory epithelium (MOE) and the vomeronasal organ (VNO). The heterotrimeric GTP-binding protein Go is widely expressed in the MOE and VNO of mice. Early studies indicated that Go expression in VNO sensory neurons is critical for directing social and sexual behaviors in female mice [Oboti L, et al. (2014) BMC Biol 12:31]. However, the physiological functions of Go in the MOE have remained poorly defined. Here, we examined the role of Go in the MOE using mice lacking the α subunit of Go Development of the olfactory bulb (OB) was perturbed in mutant mice as a result of reduced neurogenesis and increased cell death. The balance between cell types of OB interneurons was altered in mutant mice, with an increase in the number of tyrosine hydroxylase-positive interneurons at the expense of calbindin-positive interneurons. Sexual behavior toward female mice and preference for female urine odors by olfactory sensory neurons in the MOE were abolished in mutant male mice. Our data suggest that Go signaling is essential for the structural and functional integrity of the MOE and for specification of OB interneurons, which in turn are required for the transmission of pheromone signals and the initiation of mating behavior with the opposite sex.

Published
2016

8. Development of the main olfactory system and main olfactory epithelium-dependent male mating behavior are altered in Go-deficient mice.

Author

Jung-Mi Choi, Sung-Soo Kim, Chan-Il Choi, Hye Lim Cha, Huy-Hyen Oh, Sungho Ghil, Young-Don Lee, Birnbaumer, Lutz, and Haeyoung Suh-Kim

Subjects
OLFACTORY nerve, EPITHELIUM, MICE reproduction, DEVELOPMENTAL neurobiology, CALBINDIN, INTERNEURONS
Abstract

In mammals, initial detection of olfactory stimuli is mediated by sensory neurons in the main olfactory epithelium (MOE) and the vomeronasal organ (VNO). The heterotrimeric GTP-binding protein Go is widely expressed in the MOE and VNO of mice. Early studies indicated that Go expression in VNO sensory neurons is critical for directing social and sexual behaviors in female mice [Oboti L, et al. (2014) BMC Biol 12:31]. However, the physiological functions of Go in the MOE have remained poorly defined. Here, we examined the role of Go in theMOE using mice lacking the a subunit of Go. Development of the olfactory bulb (OB) was perturbed in mutant mice as a result of reduced neurogenesis and increased cell death. The balance between cell types of OB interneurons was altered in mutant mice, with an increase in the number of tyrosine hydroxylase-positive interneurons at the expense of calbindin-positive interneurons. Sexual behavior toward female mice and preference for female urine odors by olfactory sensory neurons in theMOE were abolished in mutant male mice. Our data suggest that Go signaling is essential for the structural and functional integrity of the MOE and for specification of OB interneurons, which in turn are required for the transmission of pheromone signals and the initiation of mating behavior with the opposite sex. [ABSTRACT FROM AUTHOR]

Published
2016
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