1. Tuning ultrasmall theranostic nanoparticles for MRI contrast and radiation dose amplification
- Author
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Brown, Needa, Rocchi, Paul, Carmès, Léna, Guthier, Romy, Iyer, Meghna, Seban, Léa, Morris, Toby, Bennett, Stephanie, Lavelle, Michael, Penailillo, Johany, Carrasco, Ruben, Williams, Chris, Huynh, Elizabeth, Han, Zhaohui, Kaza, Evangelia, Doussineau, Tristan, Toprani, Sneh M, Qin, Xingping, Nagel, Zachary D, Sarosiek, Kristopher A, Hagège, Agnès, Dufort, Sandrine, Bort, Guillaume, Lux, François, Tillement, Olivier, and Berbeco, Ross
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Condensed Matter - Materials Science - Abstract
Background: The introduction of magnetic resonance (MR)-guided radiation treatment planning has opened a new space for theranostic nanoparticles to reduce acute toxicity while improving local control. In this work, second-generation AGuIX nanoparticles (AGuIX-Bi) are synthesized and validated. AGuIX-Bi are shown to maintain MR positive contrast while further amplifying the radiation dose by the replacement of some Gd$^{3+}$ cations with higher Z Bi$^{3+}$. These next-generation nanoparticles are based on the AGuIX platform, which is currently being evaluated in multiple Phase II clinical trials in combination with radiotherapy. Methods: In this clinically scalable methodology, AGuIX is used as an initial chelation platform to exchange Gd$^{3+}$ for Bi$^{3+}$. AGuIX-Bi nanoparticles are synthesized with three ratios of Gd/Bi, each maintaining MR contrast while further amplifying radiation dose relative to Bi$^{3+}$. Safety, efficacy, and theranostic potential of the nanoparticles were evaluated in vitro and in vivo in a human non-small cell lung cancer model. Results: We demonstrated that increasing Bi$^{3+}$ in the nanoparticles is associated with more DNA damage and improves in vivo efficacy with a statistically significant delay in tumor growth and 33% complete regression for the largest Bi/Gd ratio tested. The addition of Bi$^{3+}$ by our synthetic method leads to nanoparticles that present slightly altered pharmacokinetics and lengthening of the period of high tumor accumulation with no observed evidence of toxicity. Conclusions: We confirmed the safety and enhanced efficacy of AGuIX-Bi with radiation therapy at the selected ratio of 30Gd/70Bi. These results provide crucial evidence towards patient translation.
- Published
- 2023
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