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In this paper, the theoretical analysis of the position estimation error in position-sensorless operation using pulsating square wave signal injection is presented. The purpose of this paper is to analyze the phenomenon that the position estimation performance varies depending on the injection frequency. Mathematical derivation supported by simulation and experimental results shows that, in no-load condition, the voltage distortion induces position estimation error with 3rd-order harmonics if the frequency of the injected square wave is the same to the switching frequency. While, if the frequency is a half of the switching frequency or heavier load condition in either injection frequency, the effect of nonideal characteristics of inverter disappears and there is no 3rd harmonics in the position estimation error. Therefore, only the effect of cross-coupling inductance is reflected in the estimation error. As the result, the position estimation error appears in the form of 6th-order harmonics due to the spatial harmonics of inductances, and the error is relatively small compared with the switching frequency injection case. The theoretical analysis was verified by simulation and experimental results. [ABSTRACT FROM AUTHOR]

Abstract The common bed bug, Cimex lectularius , possesses a cholinesterase expressed exclusively in the salivary gland (ClSChE). In this study, we investigated the molecular forms, tissue distribution patterns and biochemical properties of ClSChE and showed that ClSChE exists as a soluble monomeric form or a soluble dimeric form connected by a disulfide bridge. Immunohistochemical analysis confirmed that ClSChE was expressed in the epithelial cells of both the salivary gland and the duct. In addition, the secretion of monomeric ClSChE through the proboscis during feeding was confirmed by western blotting using a ClSChE-specific antibody. To predict the role of ClSChE injected into the tissue of an animal host, we analyzed the extent of hydrolysis of acetylcholine (ACh) by ClSChE by ultra-performance liquid chromatography-tandem mass spectrometry. ClSChE binding to ACh was not clearly resolved in the binding assay format used in this study, probably due to the weak but detectable ACh-hydrolytic activity of ClSChE. Nevertheless, kinetic analysis revealed that ClSChE possesses extremely low K m (high affinity to ACh) and V max values. These findings suggest that ClSChE functions virtually as an ACh-sequestering protein by having a very strong affinity to ACh but an extremely long turnover time. Given that ACh regulates a wide variety of host physiologies, we discuss the tentative roles of ClSChE in blood vessel constriction and itch/pain regulation in the host. Graphical abstract Image 1 Highlights • ClSChE is expressed in the epithelial cells of both salivary gland and duct. • Monomeric form of ClSChE is secreted through proboscis during feeding. • UPLC-MS/MS-based kinetic analysis reveals extremely low K m and V max of ClSChE. • ClSChE appears to function virtually as acetylcholine-sequestration protein. • Putative roles of ClSChE in blood feeding are discussed. [ABSTRACT FROM AUTHOR]

In this paper, the characteristics of ripple current of a multimegawatt drive system fed by a pulse-width modulation (PWM) inverter are investigated. Based on the investigation, a novel PWM scheme is devised to reduce the switching loss of the inverter under an identical amount of torque ripple of a motor. The proposed PWM is composed of two parts. The first is minimum torque ripple PWM (MTRPWM), which is optimized in terms of the minimum torque ripple in high modulation index (MI). The second determines the minimum switching frequency while holding the average torque ripple less than the allowable value under various operating conditions. It is termed reduced switching frequency PWM (RSFPWM). Consequently, by simply changing the PWM method from conventional fixed switching frequency continuous PWM to the proposed PWM, MTRPWM and RSFPWM, simulation results show that the switching loss of the inverter driving a 3.5 MW induction motor is reduced to 51.6% while keeping the torque ripple in the allowable boundary, where MI is 1.12. This approach is applied to small-scale experimental sets consisting of an interior permanent magnet synchronous machine (IPMSM) motor-generator set, and an induction machine MG set in an effort to verify the validity and feasibility of the proposed method. Based on these experimental results, the cost indexes of the IPMSM and the induction machine, as defined in this paper, decrease by up to 66% and 77%, respectively, when the proposed method is applied when MI is 1.12 compared with the outcome with the conventional PWM. [ABSTRACT FROM AUTHOR]

Highlights: [•] Molecular and enzymatic properties of ClAChE1 and ClAChE2 were investigated. [•] Both ClAChE1 and ClAChE2 are mainly distributed in the central nervous system. [•] ClAChE1 showed higher catalytic efficiency toward acetylcholine. [•] ClAChE1 is the more relevant toxicological target for organophosphates and carbamates. [Copyright &y& Elsevier]