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Precise arrangements of plasmonic nanoparticles on substrates are important for designing optoelectronics, sensors and metamaterials with rational electronic, optical and magnetic properties. Bottom-up synthesis offers unmatched control over morphology and optical response of individual plasmonic building blocks. Usually, the incorporation of nanoparticles made by bottom-up wet chemistry starts from batch synthesis of colloids, which requires time-consuming and hard-to-scale steps like ligand exchange and self-assembly. Herein, an unconventional bottom-up wet-chemical synthetic approach for producing gold nanoparticle ordered arrays is developed. Water-processable hydroxypropyl cellulose stencils facilitate the patterning of a reductant chemical ink on which nanoparticle growth selectively occurs. Arrays exhibiting lattice plasmon resonances in the visible region and near infrared (quality factors of >20) are produced following a rapid synthetic step (, The authors thank Camilla Dore for her advice and contributed expertise on the fabrication of HPC films. The authors extend their gratitude to Jose Mendoza Carreño for his assistance with the optical characterization of the gold nanoparticle arrays. This project had received funding from the Spanish Ministry of Science and Innovation through grants FUNFUTURE (CEX2019-000917-S), (FUNFUTURE, in the framework of the Spanish Severo Ochoa Centre of Excellence program) and PID2019-106860GB-I00 (HIGHN). L.S. and A.M. thank the Spanish National Research Council (CSIC) for funding via the I-LINK 2020 international travel grant, which facilitated international exchange period necessary for completion of this work. L.S. and Y.C. research is supported by the 2020 Post-doctoral Junior Leader-Incoming Fellowship by “la Caixa” Foundation (ID 100010434, fellow-ship code LCF/BQ/PI20/11760028). Y.C. acknowledges the auspices of the UAB material science doctoral program. G.A.V.W. thanks the UCLA graduate division for funding through the University of California Office of the President Dissertation Year Fellowship. S.J.J. acknowledges support from the National Institutes of Health (NIH) Common Fund through a NIH Director's Early Independence Award co-funded by the National Institute of Dental and Craniofacial Research and Office of the Director, NIH Grant DP5OD028181. S.J.J. and G.A.V.W. acknowledge support through a Scholar Award from the Hyundai Hope on Wheels Foundation for Pediatric Cancer Research. P.S.W. thanks the National Science Foundation for support through Grant #CHE-2004238., With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000917-S).

Micro- and nanoscale patterned monolayers of plasmonic nanoparticles were fabricated by combining concepts from colloidal chemistry, self-assembly, and subtractive soft lithography. Leveraging chemical interactions between the capping ligands of pre-synthesized gold colloids and a polydimethylsiloxane stamp, we demonstrated patterning gold nanoparticles over centimeter-scale areas with a variety of micro- and nanoscale geometries, including islands, lines, and chiral structures (e.g., square spirals). By successfully achieving nanoscale manipulation over a wide range of substrates and patterns, we established a powerful and straightforward strategy, nanoparticle chemical lift-off lithography (NP-CLL), for the economical and scalable fabrication of functional plasmonic materials with colloidal nanoparticles as building blocks, offering a transformative solution for designing next-generation plasmonic technologies., The authors would like to thank the Nanoquim clean room facility at ICMAB-CSIC and, in particular, Dr. Luigi Morrone for help with the use of the microwriter for master preparation. We acknowledge the CNSI-EICN, ICMAB-CSIC, and ICN2 electron microscopy facilities for TEM and SEM imaging, WiTEC for access to their microscopy instrumentation, and Dr. Thomas Young and Michael Mellody for the preparation of silicon masters. Special thanks to Dr. Leonora Velleman for the fruitful discussion on nanoparticle self-assembly. L.S. research is supported by the Marie Sklodowska-Curie Actions SHINE (H2020-MSCA-IF-2019, grant agreement No. 894847) and the 2020 Post-doctoral Junior Leader-Incoming Fellowship by “la Caixa” Foundation (ID 100010434, fellowship code LCF/BQ/PI20/11760028). L.A.P. thanks the Marie Sklodowska-Curie Actions (H2020-MSCA-IF-2018) for grant agreement No. 839402, PLASMIONICO. L.S., L.A.P., C.D., and A.M. acknowledge funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (Grant Agreement No. 637116, ENLIGHTMENT). ICMAB acknowledges the Spanish Ministry of Economy and Competitiveness under grants PID2019-106860GB-I00 (AEI/FEDER, UE) and FUNFUTURE (CEX2019-000917-S) center of excellence Severo Ochoa program. This work has been performed in the framework of the doctorate in Materials Science of the Autonomous University of Barcelona. N.C. thanks the NIH NIBIB Pathway to Independence Award (K99EB028325) for support. S.J.J. is supported by the NIH Common Fund through a NIH Director’s Early Independence Award co-funded by the National Institute of Dental and Craniofacial Research and Office of the Director, NIH Grant DP5OD028181. S.J.J. also acknowledges Young Investigator Award funds from the Alex’s Lemonade Stand Foundation for Childhood Cancer Research and the Hyundai Hope on Wheels Foundation for Pediatric Cancer Research. P.S.W. thanks the National Science Foundation (2004238) for support of this work. Funds for core facility use were provided via a support voucher awarded to N.C. and S.J.J. via the UCLA Clinical and Translational Science Institute (CTSI) Core Voucher Program, which is administered through Grant Number UL1TR001881.

© 2021 Rodríguez-Hernández, Casado-García, Isidro-Hernández, Picard, Raboso-Gallego, Alemán-Arteaga, Orfao, Blanco, Riesco, Prieto-Matos, García Criado, García Cenador, Hock, Enver, Sanchez-Garcia and Vicente-Dueñas., ETV6-RUNX1 is almost exclusively associated with childhood B-cell acute lymphoblastic leukemia (B-ALL), but the consequences of ETV6-RUNX1 expression on cell lineage decisions during B-cell leukemogenesis are completely unknown. Clinically silent ETV6-RUNX1 preleukemic clones are frequently found in neonatal cord blood, but few carriers develop B-ALL as a result of secondary genetic alterations. The understanding of the mechanisms underlying the first transforming steps could greatly advance the development of non-toxic prophylactic interventions. Using genetic lineage tracing, we examined the capacity of ETV6-RUNX1 to instruct a malignant phenotype in the hematopoietic lineage by cell-specific Cre-mediated activation of ETV6-RUNX1 from the endogenous Etv6 gene locus. Here we show that, while ETV6-RUNX1 has the propensity to trigger both T- and B-lymphoid malignancies, it is the second hit that determines tumor cell identity. To instigate leukemia, both oncogenic hits must place early in the development of hematopoietic/precursor cells, not in already committed B-cells. Depending on the nature of the second hit, the resulting B-ALLs presented distinct entities that were clearly separable based on their gene expression profiles. Our findings give a novel mechanistic insight into the early steps of ETV6-RUNX1+ B-ALL development and might have major implications for the potential development of ETV6-RUNX1+ B-ALL prevention strategies., Research in CV-D group has been funded by Instituto de Salud Carlos III through the project “PI17/00167” and by a “Miguel Servet Grant” (CPII19/00024—AES 2017–2020), co-funded by European Regional Development Fund/European Social Fund (“A way to make Europe”/“Investing in your future”). Research in the IS-G group is partially supported by FEDER and SAF2015-64420-R MINECO/FEDER, UE, RTI2018-093314-B-I00 MCIU/AEI/FEDER, UE, and by Junta de Castilla y León (UIC-017, CSI001U16, CSI234P18, and CSI144P20). The IS-G lab is a member of the EuroSyStem and the DECIDE Network funded by the European Union under the FP7 program. CV-D and IS-G have been supported by the German Federal Office for Radiation Protection (BfS)–Germany (FKZ: 3618S32274). IS-G has been supported by the Fundacion Unoentrecienmil (CUNINA project). HH was supported by a Hyundai Hope on Wheels scholar grant. GR-H was supported by FSE-Conserjería de Educación de la Junta de Castilla y León (CSI001-15). AC-G and MI-H are supported by FSE-Conserjería de Educación de la Junta de Castilla y León 2019 and 2020 (ESF—European Social Fund) fellowship, respectively (REF. CSI067-18 and CSI021-19). JR-G was supported by a scholarship from the University of Salamanca, co-financed by Banco Santander and ESF. SA-A was supported by RTI2018-093314-B-I00 MCIU/AEI/FEDER fellowship (PRE2019-088887).