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236 results on '"Hyypiä, Timo"'

12. Parechovirus

Author

Stanway, Glyn, primary, Williams, Çigdem H., additional, and Hyypiä, Timo, additional

Published
2011
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25. Paramyxoviruses

Author

Vainionpää, Raija, Hyypiä, Timo, Bendinelli, Mauro, editor, Friedman, Herman, editor, and Specter, Steven, editor

Published
1989
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27. Single-cell analysis of population context advances RNAi screening at multiple levels

Author

Snijder, Berend, Sacher, Raphael, Rämö, Pauli, Liberali, Prisca, Mench, Karin, Wolfrum, Nina, Burleigh, Laura, Scott, Cameron C, Verheije, Monique H, Mercer, Jason, Moese, Stefan, Heger, Thomas, Theusner, Kristina, Jurgeit, Andreas, Lamparter, David, Balistreri, Giuseppe, Schelhaas, Mario, De Haan, Cornelis A M, Marjomäki, Varpu, Hyypiä, Timo, Rottier, Peter J M, Sodeik, Beate, Marsh, Mark, Gruenberg, Jean, Amara, Ali, Greber, Urs, Helenius, Ari, Pelkmans, Lucas, LS Pathologie, LS Virologie, PB SIB, Strategic Infection Biology, University of Zurich, Pelkmans, Lucas, LS Pathologie, LS Virologie, PB SIB, and Strategic Infection Biology

Subjects
to, Image Processing, Druggability, Genome, Image analysis, 0302 clinical medicine, Computer-Assisted, SX00 SystemsX.ch, 2604 Applied Mathematics, Single-cell analysis, RNA interference, Models, 2400 General Immunology and Microbiology, Image Processing, Computer-Assisted, Viral, RNA, Small Interfering, 0303 health sciences, education.field_of_study, Applied Mathematics, Systems Biology, Genomics, 10124 Institute of Molecular Life Sciences, Cell biology, cell variability, Computational Theory and Mathematics, Cellular Microenvironment, Virus Diseases, Viruses, RNA, Viral, RNA Interference, Single-Cell Analysis, General Agricultural and Biological Sciences, Information Systems, Systems biology, Virus infection, Population, Context (language use), 1100 General Agricultural and Biological Sciences, Biology, Small Interfering, Models, Biological, General Biochemistry, Genetics and Molecular Biology, SX08 LipidX, 03 medical and health sciences, Viral Proteins, Cell-to-cell variability, Population context, RNAi, 1300 General Biochemistry, Genetics and Molecular Biology, Humans, Computer Simulation, education, 030304 developmental biology, General Immunology and Microbiology, Reproducibility of Results, Bayes Theorem, cell, Biological, 570 Life sciences, biology, RNA, 030217 neurology & neurosurgery, HeLa Cells
Abstract

Isogenic cells in culture show strong variability, which arises from dynamic adaptations to the microenvironment of individual cells. Here we study the influence of the cell population context, which determines a single cell's microenvironment, in image‐based RNAi screens. We developed a comprehensive computational approach that employs Bayesian and multivariate methods at the single‐cell level. We applied these methods to 45 RNA interference screens of various sizes, including 7 druggable genome and 2 genome‐wide screens, analysing 17 different mammalian virus infections and four related cell physiological processes. Analysing cell‐based screens at this depth reveals widespread RNAi‐induced changes in the population context of individual cells leading to indirect RNAi effects, as well as perturbations of cell‐to‐cell variability regulators. We find that accounting for indirect effects improves the consistency between siRNAs targeted against the same gene, and between replicate RNAi screens performed in different cell lines, in different labs, and with different siRNA libraries. In an era where large‐scale RNAi screens are increasingly performed to reach a systems‐level understanding of cellular processes, we show that this is often improved by analyses that account for and incorporate the single‐cell microenvironment., Molecular Systems Biology, 8 (1), ISSN:1744-4292

Published
2012
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35. Cardiomyocyte apoptosis after antiviral WIN 54954 treatment in murine coxsackievirus B3 myocarditis.

Author

Kytö, Ville, Saraste, Antti, Fohlman, Jan, Ilbäck, Nils-G., Harvala, Heli, Vuorinen, Tytti, Hyypiä, Timo, Kytö, Ville, Ilbäck, Nils-G, and Hyypiä, Timo

Subjects
HEART cells, APOPTOSIS, COXSACKIEVIRUS diseases, TREATMENT of myocarditis, THERAPEUTICS
Abstract

Objective: Cardiomyocyte apoptosis (CA) is known to occur in experimental coxsackievirus B3 (CVB3) myocarditis. However, the mechanisms of CA induction are not well known. In this study we investigate the role of direct viral induction of CA in CVB3 myocarditis.Design: A/J mice were infected with the Woodruff strain of CVB3 and treated with WIN 54954 for 5 days thereafter. WIN 54954, a compound that inhibits early events of picornavirus infection, is known to dramatically reduce mortality in murine CVB3 myocarditis without abrogating systemic or myocardial inflammation. Presence of viral RNA (in situ hybridization), CA (TUNEL method) and histopathology were studied in transverse ventricular sections at day 7 post infection (n = 8 treated and n = 8 non-treated).Results: The proportion of cardiomyocytes containing viral RNA was 89% lower in WIN 54954 treated mice when compared with non-treated mice (0.29 +/- 0.56% vs 2.76 +/- 1.65%, p = 0.003). Treatment also reduced the amount of CA by 52% compared with non-treated mice (0.20 +/- 0.06% vs 0.42 +/- 0.06%, p < 0.001). The reduction of CA by WIN treatment did not result in any increase of necrosis, in fact treatment reduced the area of necrotic lesions by 77% (2.51 +/- 1.64% vs 11.10 +/- 8.76%, p = 0.028).Conclusion: Taking the results of the reduced CA, necrosis and viral RNA with no effect on inflammation into account, our findings suggest the importance of direct viral effect in cardiomyocyte damage by both apoptosis and necrosis in CVB3 myocarditis. [ABSTRACT FROM AUTHOR]

Published
2002
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37. Guiding plant virus particles to integrin-displaying cells

Author

Hovlid, Marisa L., primary, Steinmetz, Nicole F., additional, Laufer, Burkhardt, additional, Lau, Jolene L., additional, Kuzelka, Jane, additional, Wang, Qian, additional, Hyypiä, Timo, additional, Nemerow, Glen R., additional, Kessler, Horst, additional, Manchester, Marianne, additional, and Finn, M. G., additional

Published
2012
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41. Molecular mechanism of α2β1 integrin interaction with human echovirus 1

Author

Jokinen, Johanna, primary, White, Daniel J, additional, Salmela, Maria, additional, Huhtala, Mikko, additional, Käpylä, Jarmo, additional, Sipilä, Kalle, additional, Puranen, J Santeri, additional, Nissinen, Liisa, additional, Kankaanpää, Pasi, additional, Marjomäki, Varpu, additional, Hyypiä, Timo, additional, Johnson, Mark S, additional, and Heino, Jyrki, additional

Published
2009
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