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Bulk high density MgB2 and a composite material made of a PLA matrix and MgB2 powder inclusions were in vivo tested as candidates for biodegradable materials for orthopedic implants. A rat model was used. Implants were introduced into femoral bone, in transversal and longitudinal directions. Assessment of the implant–tissue interaction was performed by X-ray imaging, X-ray computer tomography, electron microscopy, cytology, and histopathology on samples at 40 and 90 days after surgery. Both materials are biocompatible, bone and adjacent soft tissue showing good tolerance of implants. Biodegradation of MgB2 is faster than for PLA-MgB2 composite, but in both cases, it is accompanied by bone regeneration. Results suggest that use of MgB2-containing composites can promote space and time control of degradation and promotes MgB2 as a promising material for fracture repair.

Abstract Sesquipedalian mud and burnt bricks (second to third century AD) were excavated from the Roman city of Romula located in the Lower Danube Region (Olt county, Romania). Along with local soils, bricks are investigated by petrographic analysis, X-ray fluorescence (XRF), X-ray diffraction (XRD), Fourier transformed infrared spectroscopy (FT-IR), electron microscopy (SEM/EDX), X-ray microtomography (XRT), thermal analysis (DTA-TG), Mӧssbauer spectroscopy, magnetometry, colorimetry, and mechanical properties assessment. The results correlate well with each other, being useful for conservation/restoration purposes and as reference data for other ceramic materials. Remarkably, our analysis and comparison with literature data indicate possible control and wise optimization by the ancient brickmakers through the recipe, design (size, shape, and micro/macrostructure), and technology of the desired physical–chemical–mechanical properties. We discuss the Roman bricks as materials that can adapt to external factors, similar, to some extent, to modern “smart” or “intelligent” materials. These features can explain their outstanding durability to changes of weather/climate and mechanical load.

The emergence of antimicrobial resistance (AMR) is a significant threat to global food security, human health, and the future of livestock production. Higher rates of antimicrobial use in dairy farming and the sheer lack of new antimicrobials available for use focused attention on the question of how the dairy production sector contributed to the development of AMR and paved the path toward taking action to curtail it on the targeted type of farms. This paper aims to provide an introduction to a phenomenon that has gained considerable attention in the recent past due to its ever-increasing impact, the use of antimicrobial drugs, the emergence of antimicrobial resistance (AMR) on dairy farms, and seeks to discuss the possibilities of approaches such as digital health monitoring and precision livestock farming. Using sensors, data, knowledge, automation, etc., digital health monitoring, as well as Precision Livestock Farming (PLF), is expected to enhance health control and minimize disease and antimicrobial usage. The work presents a literature review on the current status and trends of AMR in dairy farms, an understanding of the concept of digital health monitoring and PLF, and the presentation and usefulness of digital health monitoring and PLF in preventing AMR. The study also analyses the strengths and weaknesses of adopting and incorporating digital technologies and artificial intelligence for dairy farming and presents areas for further study and level of use.

Radiation delivery at ultrahigh dose rates (UHDRs) has potential for use as a new anticancer therapeutic strategy. The FLASH effect induced by UHDR irradiation has been shown to maintain antitumour efficacy with a reduction in normal tissue toxicity; however, the FLASH effect has been difficult to demonstrate in vitro. The objective to demonstrate the FLASH effect in vitro is challenging, aiming to reveal a differential response between cancer and normal cells to further identify cell molecular mechanisms. New high-intensity petawatt laser-driven accelerators can deliver very high-energy electrons (VHEEs) at dose rates as high as 10 13  Gy/s in very short pulses (10 -13  s). Here, we present the first in vitro experiments carried out on cancer cells and normal non-transformed cells concurrently exposed to laser-plasma accelerated (LPA) electrons. Specifically, melanoma cancer cells and normal melanocyte co-cultures grown on chamber slides were simultaneously irradiated with LPA electrons. A non-uniform dose distribution on the cell cultures was revealed by Gafchromic films placed behind the chamber slide supporting the cells. In parallel experiments, cell co-cultures were exposed to pulsed X-ray irradiation, which served as positive controls for radiation-induced nuclear DNA double-strand breaks. By measuring the impact on discrete areas of the cell monolayers, the greatest proportion of the damaged DNA-containing nuclei was attained by the LPA electrons at a cumulative dose one order of magnitude lower than the dose obtained by pulsed X-ray irradiation. Interestingly, in certain discrete areas, we observed that LPA electron exposure had a different effect on the DNA damage in healthy normal human epidermal melanocyte (NHEM) cells than in A375 melanoma cells; here, the normal cells were less affected by the LPA exposure than cancer cells. This result is the first in vitro demonstration of a differential response of tumour and normal cells exposed to FLASH irradiation and may contribute to the development of new cell culture strategies to explore fundamental understanding of FLASH-induced cell effect., (© 2024. The Author(s).)

The innovative strategy of using nanoparticles in radiotherapy has become an exciting topic due to the possibility of simultaneously improving local efficiency of radiation in tumors and real-time monitoring of the delivered doses. Yttrium oxide (Y 2 O 3 ) nanoparticles (NPs) are used in material science to prepare phosphors for various applications including X-ray induced photodynamic therapy and in situ nano-dosimetry, but few available reports only addressed the effect induced in cells by combined exposure to different doses of superficial X-ray radiation and nanoparticles. Herein, we analyzed changes induced in melanoma cells by exposure to different doses of X-ray radiation and various concentrations of Y 2 O 3 NPs. By evaluation of cell mitochondrial activity and production of intracellular reactive oxygen species (ROS), we estimated that 2, 4, and 6 Gy X-ray radiation doses are visibly altering the cells by inducing ROS production with increasing the dose while at 6 Gy the mitochondrial activity is also affected. Separately, high-concentrated solutions of 25, 50, and 100 µg/mL Y 2 O 3 NPs were also found to affect the cells by inducing ROS production with the increase of concentration. Additionally, the colony-forming units assay evidenced a rather synergic effect of NPs and radiation. By adding the NPs to cells before irradiation, a decrease of the number of proliferating cell colonies was observed with increase of X-ray dose. DNA damage was evidenced by quantifying the γ-H2AX foci for cells treated with Y 2 O 3 NPs and exposed to superficial X-ray radiation. Proteomic profile confirmed that a combined effect of 50 µg/mL Y 2 O 3 NPs and 6 Gy X-ray dose induced mitochondria alterations and DNA changes in melanoma cells.

In recent years, luminescence nanothermometers with near infrared light (NIR) emission excited in the NIR range have attracted much attention due to their potential in bio applications. Here, we propose a new nanothermometer based on triple doped 1%Ho, 1%Er, 1%Yb - Y 2 O 3 that operates in the second and third biological windows around 1200 and 1530 nm under pulsed excitation at 905 nm. The NIR emissions were analysed in the temperature range of 298-473 K in terms of intensity, shape and dynamics. The nanothermometer performances were described using the luminescent intensity ratio (LIR) corresponding to the 5 I 6 - 5 I 8 and 4 I 13/2 - 4 I 15/2 emissions transitions of Ho and Er, respectively. A maximum relative sensitivity of 1.5% K -1 was achieved at 309 K, which is among the highest five values reported so far for the NIR to NIR downconversion nanothermometers. The thermometer performance for biological application was assessed in terms of nanothermometer reliability and stability as well as emission shape changes induced by water and custom designed optical phantoms. Combination between use of pulsed excitation and identification of Ln doping configuration offering both excitation and emission in the biological windows represent a solid approach that can be easily translated to other hosts to develop a new class of near infrared nanothermometers.

Ionizing radiation exposure produces direct or indirect biological effects on genomic DNA. The latter are ionizing radiation mediated by induction of free radicals and oxygen species (ROS). The study was conducted to evaluate the dose-effect/time-effect of antioxidant treatments in reducing the induction of double-strand breaks in human blood lymphocytes. Human peripheral blood samples of 2 mL each from healthy donors were irradiated with 10 mGy after pre-incubation with different antioxidants (β-carotene, vitamin E, vitamin C, N-acetyl L-cysteine). In order to assess their efficiency as prophylactic therapy for irradiation, various concentrations and combinations of antioxidants, as well as different incubation times, have been evaluated. To assess double-strand breaks induced by ionizing radiation, the phosphorylated histone γ-H2AX has been used. A significant reduction (p < 0.001) in double-strand breaks studied with a γ-H2AX assay was observed with N-acetyl L-cysteine with a 1-h incubation time, followed by vitamin C, vitamin E, and β-carotene. The use of antioxidants, especially N-acetyl L-cysteine before irradiation, significantly decreased the occurrence of double-strand breaks, demonstrating the potential radiological protection for exposure to ionizing radiation.

Reduced activation ferritic and martensitic steel like EUROFER (9Cr-1W) are considered as potential structural materials for the first wall of the future next-generation DEMOnstration Power Station (DEMO) fusion reactor and as a reference material for the International Thermonuclear Experimental Reactor (ITER) test blanket module. The primary motivation of this work is to study the re-deposition of the main constituent materials of EUROFER, namely tungsten (W), iron (Fe), and chromium (Cr), in a DEMO type reactor by producing and analyzing complex W x Cr y Fe 1-x-y layers. The composite layers were produced in laboratory using the thermionic vacuum arc (TVA) method, and the morphology, crystalline structure, elemental composition, and mechanical properties were studied using scanning electron microscopy (SEM), X-ray diffraction (XRD), micro-X-ray fluorescence (micro-XRF), and glow discharge optical emission spectrometry (GDOES), as well as nanoindentation and tribology measurements. The results show that the layer morphology is textured and is highly dependent on sample positioning during the deposition process. The formation of polycrystalline W x Cr y Fe 1-x-y was observed for all samples with the exception of the sample positioned closer to Fe anode during deposition. The crystalline grain size dimension varied between 10 and 20 nm. The composition and thickness of the layers were strongly influenced by the in-situ coating position, and the elemental depth profiles show a non-uniform distribution of Fe and Cr in the layers. The highest hardness was measured for the sample positioned near the Cr anode, 6.84 GPa, and the lowest was 4.84 GPa, measured for the sample positioned near the W anode. The tribology measurements showed an abrasive sliding wear behavior for most of the samples with a reduction of the friction coefficient with the increase of the normal load.