Search

Your search keyword '"IFV Pôle Rhône-Méditerranée"' showing total 13 results
Start Over Author "IFV Pôle Rhône-Méditerranée"
13 results on '"IFV Pôle Rhône-Méditerranée"'

1. Development and validation of the SMOS-IC version 2 (V2) soil moisture product

Author

Frédéric Frappart, J. P. Wigneron, Xiangzhuo Liu, Lei Fan, Mengjia Wang, Xiaojun Li, Christophe Moisy, Amen Al-Yaari, Interactions Sol Plante Atmosphère (UMR ISPA), Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Géosciences Environnement Toulouse (GET), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), Nanjing University of Information Science and Technology (NUIST), Pôle Matériel Végétal, UMT Géno-Vigne, and IFV Pôle Rhône-Méditerranée

Subjects
validation, Vegetation optical depth, 010504 meteorology & atmospheric sciences, Moisture, Meteorology, 0211 other engineering and technologies, Biosphere, Inversion (meteorology), 02 engineering and technology, Vegetation, 01 natural sciences, Carbon cycle, vegetation optical depth, L-band, 13. Climate action, Environmental science, Product (category theory), soil moisture, SMOS-IC, [SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces, environment, Water content, 021101 geological & geomatics engineering, 0105 earth and related environmental sciences, SMOS
Abstract

International audience; Since the first version of the SMOS-IC retrieval product was released in 2017, its soil moisture (SM) and L-band Vegetation Optical depth (VOD) retrievals have proven to be a very interesting alternative product for the SMOS mission. This product relies on a two-parameter inversion of the L-MEB model (L-band Microwave Emission of the Biosphere) which is independent of auxiliary data, a key feature making it well-suited for application in hydrology, agriculture, climate, and carbon cycle. This paper describes the development and validation of the most recent SMOS-IC version (V2) soil moisture product. Compared with the previous version (V105), a new constraint was applied on VOD in the cost function which is minimized in the retrieval process. Soil moisture retrievals from SMOS-IC V2 & V105 were inter-compared against the “European Centre for Medium-Range Weather Forecasts” (ECMWF) modelled SM and the “International Soil Moisture Network” (ISMN) in-situ measurements during 2011-2017 over France. It was found that the average retrieval uncertainty of the new version product was lower than that of the old version, particularly when vegetation density increased. The new version of the SMOS-IC soil moisture product will be made available to the public through the CATDS (Centre Aval de Traitements des Données SMOS) website.

Published
2020
Full Text
View/download PDF

2. La déstabilisation colloïdale des boissons limpides : recherche sur les mécanismes impliqués et développement d'itinéraires technologiques de prévention

Author

Bauduin, Rémi, Poupard, Pascal, Cottereau, Philippe, Charrier, Frederic, Meistermann, Eric, Vernhet, Aude, Poncet-Legrand, Céline, Millet, Mélanie, Guyot, Sylvain, Lequere, J-M, Salmon, J-M, Zanchi, Drazen, UMT Actia Nova2Cidre (Domaine de la motte), Insitut Français des Productions Cidricoles (IFPC), IFV Pôle Rhône-Méditerranée, Institut Français de la Vigne et du Vin (IFV), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Expérimentale de Pech-Rouge (PECH ROUGE), Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640) (PASTEUR), Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), CasDAR, Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SPI.OTHER]Engineering Sciences [physics]/Other, procédés, interaction, mécanismes, bière, Mots-clés : Troubles colloïdaux, matériel végétal, macromolécules, vin, pommeau, cidre, réactivité, [CHIM.OTHE]Chemical Sciences/Other, jus de pomme
Abstract

Ce projet concerne les boissons limpides produites à partir de pommes à cidre (cidre, pommeau, jus de pomme), les vins (rouges et blancs) et les bières, produits qui présentent assez fréquemment une perte de limpidité néfaste pour l'appréciation du produit par le consommateur. Les résultats de recherche acquis au cours du projet ont permis d'identifier les acteurs de la déstabilisation colloïdale des différents produits mais aussi de lever, au moins partiellement, les verrous de connaissance sur les mécanismes impliqués. Ces nouvelles connaissances ont été mises à profit à la fois pour améliorer la fiabilité des outils de diagnostic prédictifs pour les praticiens, mais aussi pour proposer et optimiser les traitements curatifs ou préventifs de l'instabilité colloïdale. Abstract: Colloidal destabilization of clear beverages: research on the mechanisms involved and development of technological prevention routes This project concerns clear drinks produced from cider apples (cider, pommeau, apple juice), wines (red and white) and beers, products that quite often exhibit a loss of clarity that is detrimental to the consumer appreciation of the final product. The research results acquired during the project made it possible to identify the sources involved in the colloidal destabilization of the various products but also to remove, at least partially, the knowledge barriers on the mechanisms involved. This new knowledge has been used both to improve the reliability of predictive diagnostic tools for producers and to propose and optimize curative or preventive treatments for colloidal instability.

Published
2020
Full Text
View/download PDF

3. Terahertz Study of Wood Structure as Impacted by Grapevine Trunk Diseases

Author

A-S. Spilmont, S. Merigeaud, Cédric Moisy, P. Larignon, Christophe Consejo, J-P. Peros, Yoann Meriguet, Maida Cardoso, J. Perry, N. Dyakonova, Dominique Coquillat, Luca Varani, R. Fernandez, P. Buzatu, L. Le Cunff, Jérémi Torres, J-L. Verdeil, Christophe Goze-Bac, Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut d’Electronique et des Systèmes (IES), Térahertz, hyperfréquence et optique (TéHO), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), CIVC, Comité Champagne, Epernay, Géno-vigne® (UMT Géno-vigne®), Institut National de la Recherche Agronomique (INRA)-Institut Français de la Vigne et du Vin (IFV)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Tridilogy SARL, Saint-Gély du Fesc, Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institut Français de la Vigne et du Vin (IFV), Diversité, Adaptation et Amélioration de la Vigne [AGAP] (DAAV), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut Français de la Vigne et du Vin (IFV)-Institut National de la Recherche Agronomique (INRA), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), and IFV Pôle Rhône-Méditerranée

Subjects
[PHYS]Physics [physics], [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, Horticulture, Terahertz radiation, 15. Life on land, Biology, Trunk, ComputingMilieux_MISCELLANEOUS, [SPI.TRON]Engineering Sciences [physics]/Electronics
Abstract

Grapevine trunk diseases affect grapevines worldwide and dramatically shorten the longevity of vineyards. This study ·has successfully demonstrated the use of THz timedomain imaging to identify the grapevine wood tissues degraded by the fungi responsible for grapevine trunk diseases.

Published
2019
Full Text
View/download PDF

4. Preliminary study of ancient DNA from a 215-year-old grapevine herbarium

Author

Mauricio Velayos, J.L. Santiago, Valérie Laucou, Denis Legrand, María-Carmen Martínez, S. Boso, Jean-Michel Boursiquot, Pilar Gago, Thierry Lacombe, Misión Biológica de Galicia, Spanish National Research Council (CSIC), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Real Jardín Botánico de Madrid, Pôle Matériel Végétal, UMT Géno-Vigne, IFV Pôle Rhône-Méditerranée, and Bodegas Grupo Estevez S. A.

Subjects
0106 biological sciences, aDNA, Genetic diversity, Horticulture, Sequence repeat, 010603 evolutionary biology, 01 natural sciences, Ampelography, microsatellites, Geography, Herbarium, Ancient DNA, Vitis vinifera, Botany, historic herbarium specimens, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Botanical garden, Clemente, 010606 plant biology & botany, Food Science
Abstract

International audience; Simon de Rojas Clemente y Rubio (1777 to 1827) is considered the father of modern ampelography. He developed the first scientific method for describing grapevine varieties through the examination of material he collected in Andalusia between 1802 and 1804. The Royal Botanical Garden in Madrid conserves the material he collected and preserved (leaves and shoots). This herbarium, the oldest of all grapevine variety herbaria, is invaluable for ampelographic and other studies (such as genetic diversity, molecular characterization, or viticultural history studies) and provides unique insight into early 19th century (the pre-phylloxera era) grapevine cultivation. The present work reports a DNA extraction protocol and a redefining of the simple sequence repeat primers routinely used in grapevine characterization, which allowed successful amplification of the ancient DNA extracted from Clemente's herborized material.

Published
2019
Full Text
View/download PDF

5. VITIRAMA: A program to characterize disease susceptibility in French ampelographic collections

Author

Viviana Rodriguez Cano, Thierry Lacombe, Cécile Marchal, Sabine Merdinoglu-Wiedemann, Yobrégat, O., Sereno, C., Moisy, C., Le Cunff, L., Timothée Flutre, Agnes Doligez, Luc Bidel, Roberto Bacilieri, Eric Duchêne, Christophe Moisy, Lambin, P., Laurent Torregrosa, Jean-Michel Boursiquot, Jean-Pierre Peros, Domaine expérimental de Vassal (MONTP DOM VASSAL), Institut National de la Recherche Agronomique (INRA), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, V'innopôleSud-Ouest, UMT Géno-Vigne, IFV Pôle Sud-Ouest, Pôle Matériel Végétal, UMT Géno-Vigne, IFV Pôle Rhône-Méditerranée, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Strasbourg (UNISTRA)-Institut National de la Recherche Agronomique (INRA), Diversité, Adaptation et Amélioration de la Vigne [AGAP] (DAAV), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
maladie, caractérisation phénotypique, education, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, pathology, ampelographie, vigne, health care economics and organizations, grapevine
Abstract

VITIRAMA: A program to characterize disease susceptibility in French ampelographic collections. 12. International Conference on Grapevine Breeding and Genetics

Published
2018

6. Precise editing of a target base in Grapevine genome

Author

HERBERT, Léo, Laucou, Valerie, MEUNIER, Anne-Cécile, LECUNFF, Loic, Lauvergeat, Virginie, Chatelet, Philippe, LIGOUT, Théo, Périn, Christophe, This, Patrice, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Pôle Matériel Végétal, UMT Géno-Vigne, IFV Pôle Rhône-Méditerranée, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and ProdInra, Migration

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDV.SA] Life Sciences [q-bio]/Agricultural sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

7. Duravitis & VDQA programs open new breeding strategies for grapevine facing T° rises

Author

Laurent Torregrosa, Antoine Bigard, Agnes Doligez, David Lecourieux, Markus Rienth, Nathalie Luchaire, Philippe Pieri, Ratthaphon Chatbanyong, Rezk Shahood, Marc Farnos, Catherine Roux, Angelique Adiveze, Jérémy Pillet, Yannick Sire, Emmanuelle Zumstein, Mélanie Veyret, Loic Lecunff, Fatma Lecourieux, Nicolas Saurin, Bertrand Muller, Hernan Ojeda, Cléa Houel, Jean-Pierre Peros, Patrice This, Anne Pellegrino, Charles Romieu, ProdInra, Migration, Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Unité expérimentale de Pech-Rouge (PECH ROUGE), Institut National de la Recherche Agronomique (INRA), Pôle Matériel Végétal, UMT Géno-Vigne, IFV Pôle Rhône-Méditerranée, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Changement climatique, [SDE] Environmental Sciences, Viticulture, Adaptation au changement climatique, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA] Life Sciences [q-bio]/Food engineering, [SDV] Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Vigne, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

8. Toxicity of extracellular proteins from Diplodia seriata and Neofusicoccum parvum involved in grapevine Botryosphaeria dieback

Author

F. Mazet-Kieffer, C. Tarnus, Sibylle Farine, M. Goddard, Christophe Bertsch, Elodie Stempien, Hélène Laloue, Julie Chong, Florence Fontaine, M. Bénard-Gellon, Philippe Larignon, Marjorie Schmitt, Flora Pensec, Laboratoire Vigne Biotechnologie et Environnement EA 3991, Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Laboratoire d'innovation moléculaire et applications (LIMA), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), IFV Pôle Rhône-Méditerranée, and Gwangju Institute of Science and Technology (GIST)

Subjects
0106 biological sciences, [SDV]Life Sciences [q-bio], Diplodia seriata, Virulence, Context (language use), Plant Science, 01 natural sciences, Microbiology, Fungal Proteins, 03 medical and health sciences, Seriata, Ascomycota, Botany, [CHIM]Chemical Sciences, Vitis, Botryosphaeria, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Disease Resistance, Plant Diseases, 2. Zero hunger, 0303 health sciences, biology, fungi, Cell Biology, General Medicine, Phytotoxin, Botryosphaeriaceae, biology.organism_classification, Host-Pathogen Interactions, Eutypa, 010606 plant biology & botany
Abstract

Botryosphaeria dieback, esca and Eutypa dieback are three economic major grapevine trunk diseases that cause severe yield reduction in vineyards worldwide. The frequency of disease symptoms has increased considerably over the past decade, and no efficient treatment is currently available to control these diseases. The different fungi associated with grapevine trunk diseases mainly induce necrotic wood and characteristic foliar symptoms. In this context, fungi virulence factors and host invasion are not well understood. We hypothesise that extracellular proteins produced by Diplodia seriata and Neofusicoccum parvum, two causal agents associated with Botryosphaeria dieback, are virulence factors responsible for the pathogenicity. In our previous work, we demonstrated that the total extracellular compounds produced by N. parvum induced more necrosis on Chardonnay calli and triggered a different defence gene expression pattern than those produced by D. seriata. Furthermore, this aggressiveness was not clearly correlated with the production of mellein, a characteristic phytotoxin of Botryosphaeriaceae, in our in vitro calli model. To characterise other potential virulence factors and to understand the mechanisms of host invasion by the fungus, we evaluated the profile, quantity and the impact of extracellular proteins produced by these fungi on Vitis vinifera calli necrosis and defence gene expression. Our results reveal that, under the same conditions, N. parvum produces more extracellular proteins and in higher concentrations than D. seriata. With Vitis vinifera cv. Chardonnay cells, we showed that equivalent concentrations of proteins secreted by N. parvum were more aggressive than those of D. seriata in producing necrosis and that they clearly induced more grapevine defence genes.

Published
2015
Full Text
View/download PDF

9. Physiological and developmental disturbances caused by Botryosphaeria dieback in the annual stems of grapevine.

Author

Moret F, Jacquens L, Larignon P, Clément G, Coppin C, Noirot E, Courty PE, Fontaine F, Adrian M, and Trouvelot S

Abstract

Botryosphaeria dieback is a grapevine trunk disease caused by fungi of the Botryosphaeriaceae family, which attacks more specifically the woody tissues. The infection leads to different symptoms including a severe form with a leaf drop as well as premature plant death. Botryosphaeria dieback causes major economic losses, since no effective treatment is yet available. A better understanding is necessary to find solutions to fight this disease. In this study, our objective was to characterize the "leaf drop" form by (1) looking for the presence of pathogens in the basal internodes of stems, (2) quantifying blocked vessels by tylosis and/or gummosis, and (3) describing the impact of the disease on vine physiology (gene expression and metabolome) and development (establishment and functioning of the cambium and phellogen) at the level of xylem and phloem of basal stem internodes. Our study has shown that Botryosphaeriaceae were present in both phloem and xylem of the basal internodes of the annual stem, with xylem vessels obturated. We have also clearly demonstrated that gene expression and metabolite profiles were strongly modified in both xylem and phloem of diseased plants. Differences in stems between healthy (control, C) and diseased (D) plants were low at flowering (vines not yet symptomatic), higher at the onset of symptom expression and still present, although less marked, at full disease expression. qRT-PCR analysis showed in both phloem and xylem an overexpression of genes involved in plant defense, and a repression of genes related to meristematic activity (i.e. vascular cambium and phellogen). Metabolomic analysis showed specific fingerprints in stems of healthy and diseased plants from the onset of symptom expression, with an increase of the level of phytoalexins and mannitol, and a decrease of 1-kestose one. At the structural level, many alterations were observed in internodes, even before the onset of symptoms: a classical obstruction of xylem vessels and, for the first time, a disorganization of the secondary phloem with an obstruction of the sieve plates by callose. The disease modifies the development of both secondary phloem (liber) and phellogen. Altogether, this study combining different approaches allowed to highlight deep vine dysfunction in the internodes at the base of stems, that may explain vine decline due to Botryosphaeria dieback., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Moret, Jacquens, Larignon, Clément, Coppin, Noirot, Courty, Fontaine, Adrian and Trouvelot.)

Published
2024
Full Text
View/download PDF

10. Sodium arsenite-induced changes in the wood of esca-diseased grapevine at cytological and metabolomic levels.

Author

Trouvelot S, Lemaitre-Guillier C, Vallet J, Jacquens L, Douillet A, Harir M, Larignon P, Roullier-Gall C, Schmitt-Kopplin P, Adrian M, and Fontaine F

Abstract

In the past, most grapevine trunk diseases (GTDs) have been controlled by treatments with sodium arsenite. For obvious reasons, sodium arsenite was banned in vineyards, and consequently, the management of GTDs is difficult due to the lack of methods with similar effectiveness. Sodium arsenite is known to have a fungicide effect and to affect the leaf physiology, but its effect on the woody tissues where the GTD pathogens are present is still poorly understood. This study thus focuses on the effect of sodium arsenite in woody tissues, particularly in the interaction area between asymptomatic wood and necrotic wood resulting from the GTD pathogens' activities. Metabolomics was used to obtain a metabolite fingerprint of sodium arsenite treatment and microscopy to visualize its effects at the histo-cytological level. The main results are that sodium arsenite impacts both metabolome and structural barriers in plant wood. We reported a stimulator effect on plant secondary metabolites in the wood, which add to its fungicide effect. Moreover, the pattern of some phytotoxins is affected, suggesting the possible effect of sodium arsenite in the pathogen metabolism and/or plant detoxification process. This study brings new elements to understanding the mode of action of sodium arsenite, which is useful in developing sustainable and eco-friendly strategies to better manage GTDs., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Trouvelot, Lemaitre-Guillier, Vallet, Jacquens, Douillet, Harir, Larignon, Roullier-Gall, Schmitt-Kopplin, Adrian and Fontaine.)

Published
2023
Full Text
View/download PDF

11. The Combination of Both Heat and Water Stresses May Worsen Botryosphaeria Dieback Symptoms in Grapevine.

Author

Fernandez O, Lemaître-Guillier C, Songy A, Robert-Siegwald G, Lebrun MH, Schmitt-Kopplin P, Larignon P, Adrian M, and Fontaine F

Abstract

(1) Background: Grapevine trunk diseases (GTDs) have become a global threat to vineyards worldwide. These diseases share three main common features. First, they are caused by multiple pathogenic micro-organisms. Second, these pathogens often maintain a long latent phase, which makes any research in pathology and symptomatology challenging. Third, a consensus is raising to pinpoint combined abiotic stresses as a key factor contributing to disease symptom expression. (2) Methods: We analyzed the impact of combined abiotic stresses in grapevine cuttings artificially infected by two fungi involved in Botryosphaeria dieback (one of the major GTDs), Neofusicoccum parvum and Diplodia seriata . Fungal-infected and control plants were subjected to single or combined abiotic stresses (heat stress, drought stress or both). Disease intensity was monitored thanks to the measurement of necrosis area size. (3) Results and conclusions: Overall, our results suggest that combined stresses might have a stronger impact on disease intensity upon infection by the less virulent pathogen Diplodia seriata . This conclusion is discussed through the impact on plant physiology using metabolomic and transcriptomic analyses of leaves sampled for the different conditions.

Published
2023
Full Text
View/download PDF

12. Impacts of Sodium Arsenite on Wood Microbiota of Esca-Diseased Grapevines.

Author

Bruez E, Larignon P, Bertsch C, Robert-Siegwald G, Lebrun MH, Rey P, and Fontaine F

Abstract

Although sodium arsenite was widely used in Europe until its ban in 2003, its effects on microorganisms is not clearly understood. To improve our understanding of sodium arsenite curative effect on GTDs, grapevines displaying esca-foliar symptoms from different French regions (Alsace, Champagne, Languedoc) were treated or not with sodium arsenite, and analyzed for their wood microbiota. Using metabarcoding, we identified the fungal and bacterial taxa composition of microbiota colonizing woody trunk tissues. Large differences in fungal microbiota composition between treated and untreated grapevines were observed while no major impacts were observed on bacteria microbiota. The main fungal species detected in untreated necrotic woody tissues was Fomitiporia mediterranea (63-94%), a fungal pathogen associated with esca. The relative abundance of this fungal species significantly decreased after sodium arsenite treatment in the three vineyards, in particular in white-rot necrotic tissues and their borders (-90%). F. mediterranea was the most sensitive to sodium arsenite among fungi from grapevine woody tissues. These results strongly suggest that the effect of sodium arsenite on GTDs is due to its ability to efficiently and almost specifically eliminate F. mediterranea from white-rot necrotic tissues, allowing saprobic fungi to colonize the tissues previously occupied by this pathogenic fungus.

Published
2021
Full Text
View/download PDF

13. Sodium arsenite effect on Vitis vinifera L. Physiology.

Author

Songy A, Vallet J, Gantet M, Boos A, Ronot P, Tarnus C, Clément C, Larignon P, Goddard ML, and Fontaine F

Subjects
Photosynthesis drug effects, Plant Diseases prevention & control, Plant Leaves drug effects, Plant Leaves metabolism, Plant Roots drug effects, Plant Roots metabolism, Plant Stems drug effects, Plant Stems metabolism, Polymerase Chain Reaction, Vitis growth & development, Vitis physiology, Arsenites pharmacology, Sodium Compounds pharmacology, Vitis drug effects
Abstract

Sodium arsenite (NaAsO 2 ) was especially used as a dormant spray to control grapevine trunk diseases (GTDs) in European vineyards until 2003 when it was banned. It was an efficient product but it was banned due to high risk for human health and the environment. Now, as one of the consequences with climatic changes, GTDs threaten the sustainability of vineyards since no similar and efficacious sprays are presently available to reduce the impact of GTDs. Research efforts were devoted to identify other active ingredients and biological control agents but they remained limited in term of efficacy. New solutions might follow from a better understanding of the modes of action of sodium arsenite which are currently lacking, specially its impact on grapevine physiology. For this study, grafted plants cv. Tempranillo were sprayed by sodium arsenite at the end of the winter. During the vegetative period, the impact on plant physiology was studied by measurement of the photosynthetic activity, the vine growth and development, and some defense responses. Our results showed that arsenic was translocated throughout the vine with an increasing gradient from the leaves to the root system, that photosynthesis was firstly reduced and then stimulated, and that plant tolerance responses were induced especially antioxidant system. The activation of grapevine defense responses by sodium arsenite could be a complementary action to fight fungal pathogens in addition to the fungicide effect., (Copyright © 2019 Elsevier GmbH. All rights reserved.)

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results