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3 results on '"IVM-IVF"'

1. Porcine circovirus type 2 detection in in vitro produced porcine blastocysts after virus sperm exposure.

Author

Galeati, Giovanna, Zannoni, Augusta, Spinaci, Marcella, Bucci, Diego, Ostanello, Fabio, Panarese, Serena, Tamanini, Carlo, and Sarli, Giuseppe

Subjects
*CIRCOVIRUS diseases, *BLASTOCYST, *PARTHENOGENESIS in animals, *SPERMATOZOA, *FERTILIZATION in vitro, *DIAGNOSIS
Abstract

This study was aimed at assessing the capability of semen experimentally infected with porcine circovirus type 2 (PCV2) to produce porcine blastocysts PCR positive for PCV2. Embryos were obtained from in vitro maturation (IVM) and in vitro fertilization (IVF) of porcine oocytes or by parthenogenesis. Sperm suspension was exposed to PCV2b and utilized for IVF. PCV2 spiked semen did not reveal any reduction in sperm viability or motility but its ability to produce infected blastocysts was irrelevant as only one out of 15 blastocysts obtained by IVF were PCV2b; however two blastocysts were PCV2a positive. Furthermore, the presence of PCV2 was demonstrated also in embryos obtained by parthenogenesis (one out of 17 was PCV2b and one PCV2a positive). Even if PCV2 firmly attaches to the surface of spermatozoa, experimentally spiked sperm were not effective in infecting oocytes during IVF and in producing PCR positive embryos. The infected blastocysts we obtained derivedmost probably from infected oocytes recovered at the abattoir. [ABSTRACT FROM AUTHOR]

Published
2016
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2. Porcine circovirus type 2 detection in in vitro produced porcine blastocysts after virus sperm exposure

Author

GALEATI, GIOVANNA, ZANNONI, AUGUSTA, SPINACI, MARCELLA, BUCCI, DIEGO, OSTANELLO, FABIO, PANARESE, SERENA, TAMANINI, CARLO, SARLI, GIUSEPPE, Galeati, Giovanna, Zannoni, Augusta, Spinaci, Marcella, Bucci, Diego, Ostanello, Fabio, Panarese, Serena, Tamanini, Carlo, and Sarli, Giuseppe

Subjects
Circovirus, Male, Pig, urogenital system, Swine, animal diseases, Parthenogenesis, virus diseases, Virus Attachment, Fertilization in Vitro, Spermatozoa, IVM-IVF, PCV2, In Vitro Oocyte Maturation Techniques, Blastocyst, Agricultural and Biological Sciences (all), Semen, embryonic structures, Animals, Female, reproductive and urinary physiology
Abstract

This study was aimed at assessing the capability of semen experimentally infected with porcine circovirus type 2 (PCV2) to produce porcine blastocysts PCR positive for PCV2. Embryos were obtained from in vitro maturation (IVM) and in vitro fertilization (IVF) of porcine oocytes or by parthenogenesis. Sperm suspension was exposed to PCV2b and utilized for IVF. PCV2 spiked semen did not reveal any reduction in sperm viability or motility but its ability to produce infected blastocysts was irrelevant as only one out of 15 blastocysts obtained by IVF were PCV2b; however two blastocysts were PCV2a positive. Furthermore, the presence of PCV2 was demonstrated also in embryos obtained by parthenogenesis (one out of 17 was PCV2b and one PCV2a positive). Even if PCV2 firmly attaches to the surface of spermatozoa, experimentally spiked sperm were not effective in infecting oocytes during IVF and in producing PCR positive embryos. The infected blastocysts we obtained derived most probably from infected oocytes recovered at the abattoir.

Published
2014

3. Daidzein does affect progesterone secretion by pig cumulus cells but it does not impair oocytes IVM

Author

Chiara Bernardini, Carlo Tamanini, Albamaria Parmeggiani, Claudia Vallorani, Giovanna Galeati, Diego Bucci, Marcella Spinaci, Galeati G., Vallorani C., Bucci D., Bernardini C., Tamanini C., Parmeggiani A., and Spinaci M.

Subjects
medicine.medical_specialty, endocrine system, DAIDZEIN, medicine.drug_class, Swine, Embryonic Development, Biology, IVM-IVF, chemistry.chemical_compound, Human fertilization, Food Animals, Internal medicine, medicine, Animals, HSP70 Heat-Shock Proteins, Blastocyst, HSP90 Heat-Shock Proteins, RNA, Messenger, Small Animals, ESTRADIOL, Progesterone, Cumulus Cells, Equine, urogenital system, Embryogenesis, Daidzein, food and beverages, Gene Expression Regulation, Developmental, Embryo, Progesterone secretion, Oocyte, Embryo, Mammalian, Isoflavones, Endocrinology, medicine.anatomical_structure, chemistry, Estrogen, Oocytes, Animal Science and Zoology, Female
Abstract

Daidzein, an isoflavone abundant in soybeans and other legumes, displays estrogen like properties. This study was aimed at evaluating the effect of daidzein (1 and 10 M ) on nuclear and cytoplasmic maturation of pig oocytes and on steroidogenic activity of cumulus cells. Daidzein supplementation during IVM had no effect on nuclear maturation and on fertilization traits. By contrast, both concentrations significantly (P < 0.05) inhibited progesterone production by cumulus cells after 24 and 48 h of culture while they did not induce any effect on estradiol production. Furthermore, daidzein did not exert any effect on the percentage of embryos that developed to blastocyst stage, on the number of blastomeres per blastocyst, or on the level of Hsp-70 and -90 gene transcript. Overall, our data demonstrate that daidzein added during oocyte maturation does not affect pig embryo development even if it markedly inhibits progesterone production by cumulus cells. Further studies are needed to evaluate the possible effect of daidzein during embryonic development.

Published
2009

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