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157 results on '"Iaffaldano N."'

7. Concentration dependent effect of dimethylacetamide and N-methylacetamide on the quality and fertility of cryopreserved chicken semen

Author

Luisa Zaniboni, MANUELA MADEDDU, Fabio Mosca, Ahmad Abdel Sayed, Stefano Paolo Marelli, Di Iorio, M., Iaffaldano, N., and SILVIA CEROLINI

Subjects
Cryopreservation, Male, Dimethylacetamide, Trehalose, Cryoprotectant, Spermatozoa, N-methylcetamide, Cryoprotective Agents, Fertility, Embryo viability, Semen, Chicken semen, Acetamides, Seeds, Sperm Motility, Animals, Sperm cryopreservation, Chickens, Semen Preservation
Abstract

The aim of this study was to compare the effect of two permeant-cryoprotectants, dimethylacetamide (DMA) and N-methylacetamide (NMA) used at different concentrations (0%, 2%, 4%, 6%) on the quality and fertility of post-thaw rooster semen. Ejaculates were processed in 7 treatments: Lake pre-freezing+0.1 M trehalose (LPF-T) (control treatment), LPF-T+2% DMA, LPF-T+4% DMA, LPF-T+6% DMA, LPF-T+2% NMA, LPF-T+4% NMA, LPF-T+6% NMA. Sperm quality [sperm membrane integrity (SMI), motility and kinetic parameters] was assessed before and after cryopreservation. Fertility and embryo viability were recorded. Increasing both DMA and NMA concentration from 2 to 6% improved SMI, total motile sperm, progressive motile sperm (PMS), VCL, VSL and VAP values. PMS recovery rates were significantly the highest in 6% DMA, 4% NMA and 6% NMA treatments. Semen cryopreserved with DMA produced the best fertility and embryo viability at 6%; progressive lower values were recorded at lower concentrations, with no viable embryos at 2%. Semen cryopreserved with NMA showed the best fertility values at 2% and lower values were recorded at higher concentrations; live embryos were found in all NMA treatments. Finally, NMA and DMA showed a similar positive concentration dependent effect of the quality of cryopreserved semen. NMA, not DMA, provided the highest fertility and embryo viability values at the lowest 2%. Therefore, the use of NMA is recommended in order to reduce the cryoprotectant concentration, with a concomitant reduction in the risk of toxicity, providing at the same time the adequate cryoprotective action to obtain viable embryos after artificial insemination of cryopreserved chicken semen.

Published
2021

32. Effect of cryoprotectants and thawing temperatures on chicken sperm quality.

Author

Miranda, M., Kulíková, B., Vašíček, J., Olexiková, L., Iaffaldano, N., and Chrenek, P.

Subjects
CHICKEN embryos, SPERMATOZOA analysis, CRYOPROTECTIVE agents, THAWING, APOPTOSIS
Abstract

Contents: There is need for standardization of freezing–thawing protocol for rooster semen to minimize variability among results. Therefore, we aimed to compare effect of four different permeating cryoprotectants and two thawing temperatures (37 vs. 5°C) on sperm post‐thaw motility and to analyse combined effect of the best permeating cryoprotectant (P‐CPA) with one of four non‐permeating cryoprotectants (N‐CPA) on post‐thaw quality of rooster semen evaluated in vitro. Pooled semen from Ross PM3 rooster heavy line was diluted in Kobidil extender and frozen in cryoprotectant solution containing 6% dimethylacetamide, 7.5% dimethylformamide, 9% N‐methylacetamide or 8% ethylene glycol (EG) in liquid nitrogen vapours. To determine the best thawing rate, straws were thawed either at 37 or 5°C. Furthermore, samples were frozen in the presence of the best N‐CPA either with 0.75 mol/L ficoll, 0.2 mol/L sucrose, 0.2 mol/L trehalose or 0.05 mol/L glycine. Sperm motility, membrane destabilization and viability were analysed to compare different freezing–thawing conditions. In addition, morphology and ultrastructure analysis were performed to compare fresh and frozen‐thawed sperm quality. Our results indicate that the combination of EG and the thawing at 5°C improves (p ≤ .05) sperm post‐thaw motility. Moreover, ficoll addition to EG‐based freezing extender provided additional beneficial effect (p ≤ .05) on progressive movement and apoptosis incidence. Further work should evaluate different N‐CPA concentrations to improve freezing protocol. In addition, fertility evaluation and testing on different chicken lines are needed in order to contribute to animal genetic resources bank. [ABSTRACT FROM AUTHOR]

Published
2018
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48. Oxidative Stability And Quality Traits Of n-3 Pufa Enriched Chicken Meat

Author

FEDERICO SIRRI, Minelli, G., Iaffaldano, N., Tallarico, N., and Franchini, A.

Subjects
food and beverages, chicken, n-3 PUFA, oxidative stability, organoleptic properties, eye diseases, n-3 polyunsaturated fatty acids, organoleptic analysis, lipid oxidation, lipids (amino acids, peptides, and proteins), animal feeding, sense organs, lcsh:Animal culture, human activities, chicken meat, lcsh:SF1-1100
Abstract

Considering the beneficial effects of n-3 polyunsaturated fatty acids (PUFA) for human health (Leskanich and Noble, 1997) several studies have been carried out to enrich animal products with these compounds. Both eggs and poultry meat enriched with n-3 PUFA may be considered as valid n-3 PUFA alternative sources to natural occurring fish products (Meluzzi et al., 2001; Sirri et al., 2002)...

Published
2003

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