223 results on '"Ilboudo, Hamidou"'
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2. Performance of clinical signs and symptoms, rapid and reference laboratory diagnostic tests for diagnosis of human African trypanosomiasis by passive screening in Guinea: a prospective diagnostic accuracy study
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Camara, Oumou, Camara, Mamadou, Falzon, Laura Cristina, Ilboudo, Hamidou, Kaboré, Jacques, Compaoré, Charlie Franck Alfred, Fèvre, Eric Maurice, Büscher, Philippe, Bucheton, Bruno, and Lejon, Veerle
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- 2023
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3. Interleukin 21 is a marker of Human African Trypanosomiasis Infection and a contributor to pathology in mice
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Capewell, Paul, primary, Ilboudo, Hamidou, additional, Cooper, Anneli, additional, Kabore, Windingoudi Justin, additional, Noyes, Harry, additional, O Neill, Kerry, additional, Camara, Mamadou, additional, Jamonneau, Vincent, additional, MacLeod, Annette, additional, and BUCHETON, Bruno, additional
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- 2024
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4. Evaluation of the Re-emergence Risk of Human African Trypanosomiasis in the Southwestern Burkina Faso, A Gold-Bearing Mutation Area
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Somda, Martin Bienvenu, Kaboré, Jacques, Karambiri, Sheila Médina, Dama, Emilie, Dabiré, Der, Compaoré, Charlie Franck Alfred, Salou, Ernest Wendemanedgé, Ilboudo, Hamidou, Houaga, Isidore, Courtin, Fabrice, Belem, Adrien Marie Gaston, Jamonneau, Vincent, and Bengaly, Zakaria
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- 2022
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5. Evaluation of antibody responses to tsetse fly saliva in domestic animals in the sleeping sickness endemic foci of Bonon and Sinfra, Côte d'Ivoire
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Somda, Martin Bienvenu, N'Djetchi, Martial Kassi, Kaboré, Jacques, Ilboudo, Hamidou, Dama, Emilie, Boma, Soudah, Courtin, Fabrice, Poinsignon, Anne, Bengaly, Zakaria, Remoué, Franck, Belem, Adrien Marie Gaston, Bucheton, Bruno, Jamonneau, Vincent, and Koffi, Mathurin
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- 2022
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6. Boosting the impact of seasonal malaria chemoprevention (SMC) through simultaneous screening and treatment of household members of children receiving SMC in Burkina Faso: a protocol for a randomized open label trial
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Sondo, Paul, Tahita, Marc Christian, Ilboudo, Hamidou, Rouamba, Toussaint, Derra, Karim, Tougri, Gauthier, Ouédraogo, Florence, Konseibo, Béatrice Marie Adélaïde, Roamba, Eli, Otienoburu, Sabina Dahlström, Kaboré, Bérenger, Kennon, Kalynn, Ouédraogo, Kadija, Zongo, Wend-Timbe-Noma Arlette Raïssa, Bocoum, Fadima Yaya, Stepniewska, Kasia, Dhorda, Mehul, Guérin, Philippe J., and Tinto, Halidou
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- 2022
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7. Impact and operational feasibility of adding malaria infection screening using an ultrasensitive RDT for placental and fetal outcomes in an area of high IPTP-SP coverage in Burkina Faso: the ASSER MALARIA pilot study protocol
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Tahita, Marc Christian, Sondo, Paul, Kabore, Berenger, Ilboudo, Hamidou, Rouamba, Toussaint, Sanou, Hyacinthe, Ouédraogo, Kadija, Compaoré, Adélaïde, Lompo, Palpouguini, Ouedraogo, Florence, Sawadogo, Seydou, Derra, Karim, Sawadogo, Yabré Edmond, Somé, Athanase M., Nana, Macaire, Sorgho, Hermann, Traore-Coulibaly, Maminata, Bassat, Quique, and Tinto, Halidou
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- 2022
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8. Prevalence of dermal trypanosomes in suspected and confirmed cases of gambiense human African trypanosomiasis in Guinea.
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Soumah, Alseny M'mah, Camara, Mariame, Kaboré, Justin Windingoudi, Sadissou, Ibrahim, Ilboudo, Hamidou, Travaillé, Christelle, Camara, Oumou, Tichit, Magali, Kaboré, Jacques, Boiro, Salimatou, Crouzols, Aline, Ngoune, Jean Marc Tsagmo, Hardy, David, Camara, Aïssata, Jamonneau, Vincent, MacLeod, Annette, Bart, Jean-Mathieu, Camara, Mamadou, Bucheton, Bruno, and Rotureau, Brice
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AFRICAN trypanosomiasis ,SKIN biopsy ,BLOOD testing ,TRYPANOSOMA brucei ,BLOOD sampling - Abstract
The skin is an anatomical reservoir for African trypanosomes, yet the prevalence of extravascular parasite carriage in the population at risk of gambiense Human African Trypanosomiasis (gHAT) remains unclear. Here, we conducted a prospective observational cohort study in the HAT foci of Forecariah and Boffa, Republic of Guinea. Of the 18,916 subjects serologically screened for gHAT, 96 were enrolled into our study. At enrolment and follow-up visits, participants underwent a dermatological examination and had blood samples and superficial skin snip biopsies taken for examination by molecular and immuno-histological methods. In seropositive individuals, dermatological symptoms were significantly more frequent as compared to seronegative controls. Trypanosoma brucei DNA was detected in the blood of 67% of confirmed cases (22/33) and 9% of unconfirmed seropositive individuals (3/32). However, parasites were detected in the extravascular dermis of up to 71% of confirmed cases (25/35) and 41% of unconfirmed seropositive individuals (13/32) by PCR and/or immuno-histochemistry. Six to twelve months after treatment, trypanosome detection in the skin dropped to 17% of confirmed cases (5/30), whereas up to 25% of unconfirmed, hence untreated, seropositive individuals (4/16) were still found positive. Dermal trypanosomes were observed in subjects from both transmission foci, however, the occurrence of pruritus and the PCR positivity rates were significantly higher in unconfirmed seropositive individuals in Forecariah. The lower sensitivity of superficial skin snip biopsies appeared critical for detecting trypanosomes in the basal dermis. These results are discussed in the context of the planned elimination of gHAT. Author summary: The skin is a reservoir for African trypanosomes. Here, we conducted a prospective study in Forecariah and Boffa, Guinea, to estimate the proportion of skin-dwelling parasites in the population. Of the 18,916 subjects screened for HAT, 96 were enrolled into our study. Participants underwent a dermatological examination and had blood samples and superficial skin biopsies taken for examination by molecular and immuno-histological methods. In individuals seropositive for HAT, dermatological symptoms were significantly more frequent. Trypanosome DNA was detected in the blood of 67% of confirmed cases and 9% of unconfirmed seropositive individuals. However, parasites were detected in the skin of up to 71% of confirmed cases and 41% of unconfirmed seropositive individuals. After treatment, trypanosome detection in the skin dropped to 17% of confirmed cases, whereas up to 25% of unconfirmed, hence untreated, seropositive individuals were still found positive. Dermal trypanosomes were observed in subjects from both regions; however, the occurrence of itching and the PCR positivity were significantly higher in unconfirmed seropositive individuals in Forecariah. The lower sensitivity of superficial skin biopsies appeared critical for detecting trypanosomes. These results are discussed in the context of the planned elimination of HAT. [ABSTRACT FROM AUTHOR]
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- 2024
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9. OA-765 Molecular diagnostic tests specificities and their contribution for HAT postelimination monitoring in Burkina Faso and Côte d’Ivoire
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Alfred Compaoré, Charlie Franck, primary, Koné, Minayégninrin, additional, Kaboré, Jacques, additional, Ilboudo, Hamidou, additional, Bamba, Mohamed, additional, Sakande, Hassane, additional, Kaba, Dramane, additional, Gaston Belem, Adrien Marie, additional, Büscher, Philippe, additional, Lejon, Veerle, additional, and Jamonneau, Vincent, additional
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- 2023
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10. PA-730 Impact of additional screening using highly-sensitive rapid diagnostic tests and treatment combined with monthly Sulfadoxine-Pyrimethamine on LBW and peripheral malaria infection: asser malaria study
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Tahita, Marc Christian, primary, Sondo, Paul, additional, Kabore, Berenger, additional, Ilboudo, Hamidou, additional, Rouamba, Toussaint, additional, Sanou, Hyacinthe, additional, Ouedraogo, Kadija, additional, Lompo, Palpouguini, additional, Ouedraogo, Florence, additional, Sawadogo, Seydou, additional, Derra, Karim, additional, Some, Athanase M, additional, Sorgho, Hermann, additional, Bassat, Quique, additional, and Tinto, Halidou, additional
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- 2023
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11. Plasmodium falciparum gametocyte carriage in symptomatic patients shows significant association with genetically diverse infections, anaemia, and asexual stage density
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Sondo, Paul, Bihoun, Biebo, Tahita, Marc Christian, Derra, Karim, Rouamba, Toussaint, Nakanabo Diallo, Seydou, Kazienga, Adama, Ilboudo, Hamidou, Valea, Innocent, Tarnagda, Zekiba, Sorgho, Hermann, Lefèvre, Thierry, and Tinto, Halidou
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- 2021
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12. Assessment of a combined strategy of seasonal malaria chemoprevention and supplementation with vitamin A, zinc and Plumpy’Doz™ to prevent malaria and malnutrition in children under 5 years old in Burkina Faso: a randomized open-label trial (SMC-NUT)
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Sondo, Paul, Tahita, Marc Christian, Rouamba, Toussaint, Derra, Karim, Kaboré, Bérenger, Compaoré, Cheick Saïd, Ouédraogo, Florence, Rouamba, Eli, Ilboudo, Hamidou, Bambara, Estelle Aïssa, Nana, Macaire, Sawadogo, Edmond Yabré, Sorgho, Hermann, Somé, Athanase Mwinessobaonfou, Valéa, Innocent, Dahal, Prabin, Traoré/Coulibaly, Maminata, and Tinto, Halidou
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- 2021
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13. Do Cryptic Reservoirs Threaten Gambiense-Sleeping Sickness Elimination?
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Büscher, Philippe, Bart, Jean-Mathieu, Boelaert, Marleen, Bucheton, Bruno, Cecchi, Giuliano, Chitnis, Nakul, Courtin, David, Figueiredo, Luisa M., Franco, José-Ramon, Grébaut, Pascal, Hasker, Epco, Ilboudo, Hamidou, Jamonneau, Vincent, Koffi, Mathurin, Lejon, Veerle, MacLeod, Annette, Masumu, Justin, Matovu, Enock, Mattioli, Raffaele, Noyes, Harry, Picado, Albert, Rock, Kat S., Rotureau, Brice, Simo, Gustave, Thévenon, Sophie, Trindade, Sandra, Truc, Philippe, and Van Reet, Nick
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- 2018
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14. Monitoring the elimination of gambiense human African trypanosomiasis in the historical focus of Batié, South–West Burkina Faso
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Compaoré Charlie Franck Alfred, Kaboré Jacques, Ilboudo Hamidou, Thomas Lian Francesca, Falzon Laura Cristina, Bamba Mohamed, Sakande Hassane, Koné Minayégninrin, Kaba Dramane, Bougouma Clarisse, Adama Ilboudo, Amathe Ouedraogo, Belem Adrien Marie Gaston, Fèvre Eric Maurice, Büscher Philippe, Lejon Veerle, and Jamonneau Vincent
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human african trypanosomiasis ,trypanosoma brucei gambiense ,elimination ,diagnosis ,rapid diagnostic test ,specificity ,dried blood spot ,burkina faso ,Infectious and parasitic diseases ,RC109-216 - Abstract
The World Health Organisation has targeted the elimination of human African trypanosomiasis (HAT) as zero transmission by 2030. Continued surveillance needs to be in place for early detection of re-emergent cases. In this context, the performance of diagnostic tests and testing algorithms for detection of the re-emergence of Trypanosoma brucei gambiense HAT remains to be assessed. We carried out a door-to-door active medical survey for HAT in the historical focus of Batié, South–West Burkina Faso. Screening was done using three rapid diagnostic tests (RDTs). Two laboratory tests (ELISA/T. b. gambiense and immune trypanolysis) and parasitological examination were performed on RDT positives only. In total, 5883 participants were screened, among which 842 (14%) tested positive in at least one RDT. Blood from 519 RDT positives was examined microscopically but no trypanosomes were observed. The HAT Sero-K-Set test showed the lowest specificity of 89%, while the specificities of SD Bioline HAT and rHAT Sero-Strip were 92% and 99%, respectively. The specificity of ELISA/T. b. gambiense and trypanolysis was 99% (98–99%) and 100% (99–100%), respectively. Our results suggest that T. b. gambiense is no longer circulating in the study area and that zero transmission has probably been attained. While a least cost analysis is still required, our study showed that RDT preselection followed by trypanolysis may be a useful strategy for post-elimination surveillance in Burkina Faso.
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- 2022
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15. Baseline malarial and nutritional profile of children under seasonal malaria chemoprevention coverage in the health district of Nanoro, Burkina Faso
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Sondo, Paul, primary, Rouamba, Toussaint, additional, Tahita, Marc Christian, additional, Derra, Karim, additional, Kabore, Berenger, additional, Tibiri, Yssimini Nadège Guillène, additional, Kabore, Hyacinthe Abd-El Latif Faïçal, additional, Hien, So-vii Franck, additional, Ouedraogo, Florence, additional, Kazienga, Adama, additional, Ilboudo, Hamidou, additional, Rouamba, Eli, additional, Lefevre, Thiery, additional, and Tinto, Halidou, additional
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- 2023
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16. Human Leukocyte Antigen-G: A Promising Prognostic Marker of Disease Progression to Improve the Control of Human African Trypanosomiasis
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Gineau, Laure, Courtin, David, Camara, Mamadou, Ilboudo, Hamidou, Jamonneau, Vincent, Dias, Fabricio C., Tokplonou, Leonidas, Milet, Jacqueline, Mendonça, Priscila B., Castelli, Erick C., Camara, Oumou, Camara, Mariam, Favier, Benoit, Rouas-Freiss, Nathalie, Moreau, Philippe, Donadi, Eduardo A., Bucheton, Bruno, Sabbagh, Audrey, and Garcia, André
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- 2016
17. Determinants of Plasmodium falciparum multiplicity of infection and genetic diversity in Burkina Faso
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Sondo, Paul, Derra, Karim, Rouamba, Toussaint, Nakanabo Diallo, Seydou, Taconet, Paul, Kazienga, Adama, Ilboudo, Hamidou, Tahita, Marc Christian, Valéa, Innocent, Sorgho, Hermann, Lefèvre, Thierry, and Tinto, Halidou
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- 2020
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18. Trypanosoma brucei gambiense Spliced Leader RNA Is a More Specific Marker for Cure of Human African Trypanosomiasis Than T. b. gambiense DNA
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Ilboudo, Hamidou, Camara, Oumou, Ravel, Sophie, Bucheton, Bruno, Lejon, Veerle, Camara, Mamadou, Kaboré, Jacques, Jamonneau, Vincent, and Deborggraeve, Stijn
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- 2015
19. Performance of clinical signs and symptoms, rapid and laboratory diagnostic tests for diagnosis of human African trypanosomiasis by passive screening in Guinea: a non-interventional, prospective cross-sectional study
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Camara, Oumou, primary, Camara, Mamadou, additional, Falzon, Laura Cristina, additional, Ilboudo, Hamidou, additional, Kaboré, Jacques, additional, Compaoré, Charlie Franck Alfred, additional, Fèvre, Eric Maurice, additional, Büscher, Philippe, additional, Bucheton, Bruno, additional, and Lejon, Veerle, additional
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- 2022
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20. Molecular diagnosis of Plasmodium falciparum in three active foci of human African trypanosomiasis (Boffa, Dubreka and Forecariah) in Guinea
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Kabore, Jacques, Camara, Oumou, Somda, Martin Bienvenu, Ilboudo, Hamidou, Kabore, Windingoudi Justin, Ouedraogo, Kouka Zoéwentallé Thibaut, Salou, Ernest, Sakande, Hassane, Bamba, Mohamed, Belem, Adrien Marie Gaston, Bengaly, Zakaria, and Camara, Mamadou
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PCR diagnosis, prevalence malaria, human African trypanosomiasis, coastal Guinea ,PCR diagnostic, prévalence du paludisme, trypanosomiase humaine africaine, Guinée côtière - Abstract
Objectives: the overall objective of this study was to determine the prevalence of Plasmodium (P.) falciparum infection in the three foci of human African trypanosomiasis (HAT) (Boffa, Dubreka and Forecariah) in coastal Guinea using the polymerase chain reaction (PCR).Methodology and results: DNA from blood samples was extracted and amplified using pf1/2 primers. A total of 1001 individuals were included. The population was predominantly male (57.34 %) and female (42.66%) with a sex ratio of 1.34. The mean age of the study population was 34 ± 16.5 years. The overall molecular prevalence of P. falciparum infection was 17.78 % (178/1001). In the three HAT foci, the prevalences were 10.37 % (31/299) in Boffa, 20.12 % (99/492) in Dubreka and 22.86 % (48/210) in Forecariah. Women (71/178 or 39.89 %) were significantly less infected than men (107/178 or 60.11 %) (p-value = 0.0013).Conclusion and application of results: These results showed the positive PCR results in the three HAT foci. The diagnosis of malaria could be improved by using PCR to determine the true extent of the disease, but this test is higher cost comparing to classical methods. The malaria/HAT co-infection in this coastal area could be a factor of aggravation of the sleeping sickness, and should be taken into account by the HAT control programme in Guinea. Objectifs : l’objectif général de cette étude était de déterminer la prévalence de l’infection à Plasmodium (P.) falciparum dans les trois foyers de trypanosomiase humaine africaine (THA) (Boffa, Dubréka et Forécariah) en Guinée côtière en utilisant la réaction d’amplification en chaîne (PCR).Méthodologie et résultats : l’ADN des échantillons de sang a été extrait et amplifié en utilisant les amorces pf1/2. Au total, 1001 individus ont été inclus. La population était constituée essentiellement de 57,34 % d’hommes et de 42,66 % de femmes, soit un sexe ratio H/F de 1,34. L’âge moyen de la population d’étude était de 34 ans ± 16,5. La prévalence moléculaire globale de l’infection à P. falciparum était de 17,78 % (178/1001). Dans les trois foyers de THA, les prévalences ont été de 10,37 % (31/299) à Boffa, de 20,12 % (99/492) à Dubréka et de 22,86 % (48/210) à Forécariah. Les femmes (71/178 soit 39,89 %) étaient moins infectées que les hommes (107/178 soit 60,11 %) (p-value = 0,0013).Conclusion et application des résultats : Nos résultats ont montré des résultats positifs de la PCR dans les trois foyers de THA. Le diagnostic du paludisme pourrait être amélioré en utilisant la PCR pour déterminer l’étendue réelle de la maladie, mais ce test est plus coûteux que les méthodes classiques. La co-infection paludisme/THA dans cette zone côtière pourrait être un facteur d'aggravation de la maladie du sommeil, et devrait être prise en compte par le programme de lutte contre la THA en Guinée.
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- 2022
21. Immune trypanolysis test as a promising bioassay to monitor the elimination of gambiense human African trypanosomiasis
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Dama Emilie, Camara Oumou, Kaba Dramane, Koffi Mathurin, Camara Mamadou, Compaoré Charlie, Ilboudo Hamidou, Courtin Fabrice, Kaboré Jacques, N’Gouan Emmanuel Kouassi, Büscher Philippe, Lejon Veerle, Bucheton Bruno, and Jamonneau Vincent
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human african trypanosomiasis ,trypanosoma brucei gambiense ,elimination ,diagnosis ,transmission ,immune trypanolysis ,Infectious and parasitic diseases ,RC109-216 - Abstract
The World Health Organization (WHO) has set the goal of gambiense-Human African trypanosomiasis (HAT) elimination as a public health problem for 2020 and interruption of transmission in humans for 2030. In this context, it is crucial to monitor progress towards these targets using accurate tools to assess the level of transmission in a given area. The aim of this study was to investigate the relevance of the immune trypanolysis test (TL) as a population-based bioassay to evaluate Trypanosoma brucei gambiense transmission in various epidemiological contexts. Significant correlations were observed between HAT endemicity levels and the percentage of TL-positive individuals in the population. TL therefore appears to be a suitable population-based biomarker of the intensity of transmission. In addition to being used as a tool to assess the HAT status at an individual level, assessing the proportion of TL positive individuals in the population appears as a promising and easy alternative to monitor the elimination of gambiense HAT in a given area.
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- 2019
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22. Diversity of response to Trypanosoma brucei gambiense infections in the Forecariah mangrove focus (Guinea): perspectives for a better control of sleeping sickness
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Ilboudo, Hamidou, Jamonneau, Vincent, Camara, Mamadou, Camara, Oumou, Dama, Emilie, Léno, Mamadou, Ouendeno, Frédéric, Courtin, Fabrice, Sakande, Hassane, Sanon, René, Kaboré, Jacques, Coulibaly, Bamoro, N’Dri, Louis, Diarra, Abdoulaye, N’Goran, Eliezer, and Bucheton, Bruno
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- 2011
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23. Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d’Ivoire
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Traoré, Barkissa Mélika, primary, Koffi, Mathurin, additional, N’Djetchi, Martial Kassi, additional, Kaba, Dramane, additional, Kaboré, Jacques, additional, Ilboudo, Hamidou, additional, Ahouty, Bernadin Ahouty, additional, Koné, Minayégninrin, additional, Coulibaly, Bamoro, additional, Konan, Thomas, additional, Segard, Adeline, additional, Kouakou, Lingué, additional, De Meeûs, Thierry, additional, Ravel, Sophie, additional, Solano, Philippe, additional, Bart, Jean-Mathieu, additional, and Jamonneau, Vincent, additional
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- 2021
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24. Extravascular dermal trypanosomes in suspected and confirmed cases of gambiense Human African Trypanosomiasis
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Camara, Mariame, Soumah, Alseny M’mah, Ilboudo, Hamidou, Travaillé, Christelle, Clucas, Caroline, Cooper, Anneli, Kuispond Swar, Nono-Raymond, Camara, Oumou, Sadissou, Ibrahim, Calvo Alvarez, Estefania, Crouzols, Aline, Bart, Jean-Mathieu, Jamonneau, Vincent, Camara, Mamadou, MacLeod, Annette, Bucheton, Bruno, Rotureau, Brice, Ministère de la Santé [Conakry, Guinea], Hôpital Donka, Institut de Recherche en Sciences de la Santé [Ouagadougou, Burkina Faso] (IRSS), Institut de Recherche pour le Développement (IRD), Biologie cellulaire des Trypanosomes - Trypanosome Cell Biology, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), College of Medical, Veterinary and Life Sciences [Glasgow], Wellcome Trust Centre for Molecular Parasitology, Institut National de Recherche Biomédicale [Kinshasa] (INRB), Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université de Bordeaux (UB), This work was supported by the Wellcome Trust (209511/Z/17/Z), the Institut de Recherche pour le Développement, the Institut Pasteur, the French Government Investissement d'Avenir programme - Laboratoire d'Excellence Integrative Biology of Emerging Infectious Diseases (ANR-10-LABX-62-IBEID) and the French National Agency for Scientific Research (projects ANR-14-CE14-0019-01 EnTrypa and ANR-18-CE15-0012 TrypaDerm)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-14-CE14-0019,ENTRYPA,Dynamique des étapes précoces de l'infection de l'hôte mammifère par les trypanosomes africains(2014), ANR-18-CE15-0012,TrypaDerm,Comprendre la biologie des trypanosomes africains dans la peau(2018), Rotureau, Brice, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Appel à projets générique - Dynamique des étapes précoces de l'infection de l'hôte mammifère par les trypanosomes africains - - ENTRYPA2014 - ANR-14-CE14-0019 - Appel à projets générique - VALID, and APPEL À PROJETS GÉNÉRIQUE 2018 - Comprendre la biologie des trypanosomes africains dans la peau - - TrypaDerm2018 - ANR-18-CE15-0012 - AAPG2018 - VALID
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reservoir ,skin ,MESH: Guinea ,MESH: Humans ,Trypanosoma brucei gambiense ,human African trypanosomiasis ,[SDV]Life Sciences [q-bio] ,MESH: Prospective Studies ,MESH: Trypanosoma brucei gambiense ,[SDV] Life Sciences [q-bio] ,Major Articles and Commentaries ,Trypanosomiasis, African ,AcademicSubjects/MED00290 ,MESH: Trypanosomiasis, African ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Animals ,Humans ,Guinea ,MESH: Animals ,[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,Prospective Studies ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology ,[SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology - Abstract
Background The diagnosis of gambiense human African trypanosomiasis (gHAT) typically involves 2 steps: a serological screen, followed by the detection of living trypanosome parasites in the blood or lymph node aspirate. Live parasites can, however, remain undetected in some seropositive individuals, who, we hypothesize, are infected with Trypanosoma brucei gambiense parasites in their extravascular dermis. Methods To test this hypothesis, we conducted a prospective observational cohort study in the gHAT focus of Forecariah, Republic of Guinea. Of the 5417 subjects serologically screened for gHAT, 66 were enrolled into our study and underwent a dermatological examination. At enrollment, 11 seronegative, 8 unconfirmed seropositive, and 18 confirmed seropositive individuals had blood samples and skin biopsies taken and examined for trypanosomes by molecular and immunohistological methods. Results In seropositive individuals, dermatological symptoms were significantly more frequent, relative to seronegative controls. T.b. gambiense parasites were present in the blood of all confirmed cases (n = 18) but not in unconfirmed seropositive individuals (n = 8). However, T. brucei parasites were detected in the extravascular dermis of all unconfirmed seropositive individuals and all confirmed cases. Skin biopsies of all treated cases and most seropositive untreated individuals progressively became negative for trypanosomes 6 and 20 months later. Conclusions Our results highlight the skin as a potential reservoir for African trypanosomes, with implications for our understanding of this disease’s epidemiology in the context of its planned elimination and underlining the skin as a novel target for gHAT diagnostics., Live trypanosomes can remain undetected in the blood of individuals seropositive for sleeping sickness. Here, we show that they could be infected with parasites in their extravascular dermis, highlighting the skin as a potential reservoir for trypanosomes.
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- 2021
25. HLA-G 3’UTR 14 bp Insertion Is Associated with a Decreased Risk of Developing Human African Trypanosomiasis in the Côte d’Ivoire Population
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Tidou Abiba Sanogo, Bruno Bucheton, Lingué Kouakou, Laure Gineau, Ilboudo Hamidou, Enock Matovu, Simon-Pierre A. N’guetta, Thomas Konan Konan, Mathurin Koffi, Didier Sokouri, David Courtin, Vincent Jamonneau, Bernardin Ahouty, and Innocent Allepo Abe
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0303 health sciences ,education.field_of_study ,030306 microbiology ,Three prime untranslated region ,Population ,030232 urology & nephrology ,Human leukocyte antigen ,Biology ,Lower risk ,medicine.disease ,Virology ,03 medical and health sciences ,0302 clinical medicine ,Immunity ,HLA-G ,parasitic diseases ,medicine ,African trypanosomiasis ,Indel ,education - Abstract
Human African trypanosomiasis (HAT), or sleeping sickness, caused by Trypanosoma brucei gambiense, is associated with diverse clinical outcomes. Host’s genetic factors involved in immunity are potential factors that can regulate infection. Genetic polymorphisms within HLA-G could influence the level of HLA-G expression and therefore play a critical role in infection outcomes. The goal of our study was to investigate the association of 14 bp Indel HLA-G polymorphism with the susceptibility/resistance to HAT. DNA samples were collected from 119 cases, 221 controls and 43 seropositive individuals living in Ivorian HAT foci. The 14 bp Indel polymorphism was determined by PCR. Homozygous individuals for 14 bp insertion had a lower risk of progressing to active HAT (p = 0.012, OR = 0.27, 95% CI: 0.09 - 0.8). Moreover, the frequency of 14 bp insertion homozygous genotype was higher in the seropositive group (11%) than in the HAT cases group (3%) (p = 0.043, OR = 0.27, 95% CI: 0.07 - 0.99), which suggested a protective effect of 14 bp insertion homozygous genotype. Genetic polymorphisms in HLA-G may be associated with a variable risk to develop HAT. The 14 bp insertion appears to favour the occurrence of long-lasting T. b. gambiense latent infections.
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- 2019
26. Des trypanosomes dans la peau
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Travaillé, Christelle, Soumah, Alseny M’mah, Ngoune, Jean Marc Tsagmo, Camara, Mariame, Ilboudo, Hamidou, Camara, Oumou, Sadissou, Ibrahim, Bart, Jean-Mathieu, Jamonneau, Vincent, Camara, Mamadou, Bucheton, Bruno, Rotureau, Brice, HORIZON, IRD, Biologie cellulaire des Trypanosomes - Trypanosome Cell Biology, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Ministère de la Santé [Conakry, Guinea], Hôpital National Donka, Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université de Bordeaux (UB), Institut Pasteur de Guinée, and Réseau International des Instituts Pasteur (RIIP)
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réservoir ,niche anatomique ,[SDV] Life Sciences [q-bio] ,[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology ,peau ,[SDV]Life Sciences [q-bio] ,Trypanosoma brucei gambiense ,transmission ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,trypanosomiase humaine africaine - Abstract
Le trypanosome africain Trypanosoma brucei gambiense, parasite responsable de la trypanosomiase humaine africaine, est transmis à l'homme par la piqûre d'une mouche tsé-tsé infectée, puis il prolifère dans le sang et la lymphe et enfin dans le système nerveux central. Le diagnostic de trypanosomiase implique deux étapes : un dépistage sérologique suivi de la détection de parasites vivants dans un fluide biologique. Cependant, les parasites peuvent rester indétectables chez certains individus qualifiés de séropositifs non confirmés qui restent alors sans traitement. Dans différents modèles de laboratoire, des chercheurs ont récemment démontré l'existence de réservoirs anatomiques de parasites extravasculaires, en particulier la peau, qui pourraient expliquer les infections latentes. Récemment, la présence de trypanosomes extravasculaires dermiques a également été démontrée chez l'homme, soulignant l'importance des individus séropositifs non confirmés comme réservoir de parasites qui pourrait compromettre l'élimination de la maladie. Les impacts cliniques et épidémiologiques de ces trypanosomes dermiques sont ici discutés.
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- 2021
27. Assessment of a combined strategy of seasonal malaria chemoprevention and supplementation with Vitamin A, Zinc and Plumpy’DozTM to prevent malaria and malnutrition in children under five years old in Burkina Faso: a randomized open label trial (SMC-NUT)
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Sondo, Paul, primary, TAHITA, Marc Christian, additional, ROUAMBA, Toussaint, additional, DERRA, Karim, additional, KABORE, Berenger, additional, COMPAORE, Cheick Saïd, additional, OUEDRAOGO, Florence, additional, ROUAMBA, Eli, additional, ILBOUDO, Hamidou, additional, BAMBARA, Estelle, additional, NANA, Macaire, additional, SAWADOGO, Edmond, additional, SORGHO, Herman, additional, SOME, Athanase Mwinessobaonfou, additional, VALEA, Innocent, additional, DAHAL, Prabin, additional, TRAORE/COULIBALY, Maminata, additional, and TINTO, Halidou, additional
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- 2021
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28. Immune trypanolysis test with blood spotted on filter paper for epidemiological surveillance of sleeping sickness
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Camara, Oumou, Camara, Mamadou, Lejon, Veerle, Ilboudo, Hamidou, Sakande, Hassane, Léno, Mamadou, Büscher, Philippe, Bucheton, Bruno, and Jamonneau, Vincent
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- 2014
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29. Polymorphisme de Plasmodium falciparum et mutations des gènes de résistance Pfcrt et Pfmdr1 dans la zone de Nanoro, Burkina Faso
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Sondo, Paul, primary, Bihoun, Biebo, additional, Kabore, Bérenger, additional, Tahita, Marc Christian, additional, Derra, Karim, additional, Rouamba, Toussaint, additional, Diallo, Seydou Nakanabo, additional, Kazienga, Adama, additional, Ilboudo, Hamidou, additional, Valea, Innocent, additional, Tarnagda, Zekiba, additional, Sorgho, Hermann, additional, Lefevre, Thierry, additional, and Tinto, Halidou, additional
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- 2021
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30. A targeted door-to-door strategy for sleeping sickness detection in low-prevalence settings in Côte d’Ivoire
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Koffi Mathurin, N’Djetchi Martial, Ilboudo Hamidou, Kaba Dramane, Coulibaly Bamoro, N’Gouan Emmanuel, Kouakou Lingué, Bucheton Bruno, Solano Philippe, Courtin Fabrice, Ehrhardt Stephan, and Jamonneau Vincent
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Human African trypanosomiasis ,Trypanosoma brucei gambiense ,Diagnosis ,Elimination strategy ,Côte d’Ivoire ,Medical survey ,Infectious and parasitic diseases ,RC109-216 - Abstract
Significant efforts to control human African trypanosomiasis (HAT) over the three past decades have resulted in drastic reductions of disease prevalence in Côte d’Ivoire. In this context, the costly and labor-intensive active mass screening strategy is no longer efficient. In addition to a more cost-effective passive surveillance system being implemented in this low-prevalence context, our aim was to develop an alternative targeted active screening strategy. In 2012, we carried out a targeted door-to-door (TDD) survey focused on the immediate vicinities of former HAT patients detected in the HAT focus of Bonon and compared the results to those obtained during classical active mass screening (AMS) surveys conducted from 2000 to 2012 in the same area. The TDD that provides a friendlier environment, inviting inhabitants to participate and gain awareness of the disease, detected significantly more HAT cases than the AMS. These results suggest that the TDD is an efficient and useful strategy in low-prevalence settings where very localized transmission cycles may persist and, in combination with passive surveillance, could help in eliminating HAT.
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- 2016
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31. Analytical sensitivity of loopamp and quantitative real-time PCR on dried blood spots and their potential role in monitoring human African trypanosomiasis elimination
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Alfred Compaoré, Charlie Franck, primary, Ilboudo, Hamidou, additional, Kaboré, Jacques, additional, Kaboré, Justin Windingoudi, additional, Camara, Oumou, additional, Bamba, Mohamed, additional, Sakande, Hassane, additional, Koné, Minayégninrin, additional, Camara, Mamadou, additional, Kaba, Dramane, additional, Gaston Belem, Adrien Marie, additional, Deborggraeve, Stijn, additional, Büscher, Philippe, additional, Bucheton, Bruno, additional, Lejon, Veerle, additional, and Jamonneau, Vincent, additional
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- 2020
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32. High Levels of Genetic Diversity within Nilo-Saharan Populations: Implications for Human Adaptation
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Mulindwa, Julius, primary, Noyes, Harry, additional, Ilboudo, Hamidou, additional, Pagani, Luca, additional, Nyangiri, Oscar, additional, Kimuda, Magambo Phillip, additional, Ahouty, Bernardin, additional, Asina, Olivier Fataki, additional, Ofon, Elvis, additional, Kamoto, Kelita, additional, Kabore, Justin Windingoudi, additional, Koffi, Mathurin, additional, Ngoyi, Dieudonne Mumba, additional, Simo, Gustave, additional, Chisi, John, additional, Sidibe, Issa, additional, Enyaru, John, additional, Simuunza, Martin, additional, Alibu, Pius, additional, Jamonneau, Vincent, additional, Camara, Mamadou, additional, Tait, Andy, additional, Hall, Neil, additional, Bucheton, Bruno, additional, MacLeod, Annette, additional, Hertz-Fowler, Christiane, additional, Matovu, Enock, additional, Mumba, Dieuodonne, additional, Alibu, Vincent P., additional, Macleod, Annette, additional, Hertzfowler, Christianne, additional, Elliot, Alison, additional, Bishop, Ozlem, additional, Mulindwa, Julius, additional, Ahouty, Bernadin, additional, and Kabore, Justin, additional
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- 2020
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33. Extravascular Dermal Trypanosomes in Suspected and Confirmed Cases of gambiense Human African Trypanosomiasis
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Camara, Mariame, primary, Soumah, Alseny M’mah, additional, Ilboudo, Hamidou, additional, Travaillé, Christelle, additional, Clucas, Caroline, additional, Cooper, Anneli, additional, Kuispond Swar, Nono-Raymond, additional, Camara, Oumou, additional, Sadissou, Ibrahim, additional, Calvo Alvarez, Estefania, additional, Crouzols, Aline, additional, Bart, Jean-Mathieu, additional, Jamonneau, Vincent, additional, Camara, Mamadou, additional, MacLeod, Annette, additional, Bucheton, Bruno, additional, and Rotureau, Brice, additional
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- 2020
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- View/download PDF
34. Sleeping sickness diagnosis: use of buffy coats improves the sensitivity of the mini anion exchange centrifugation test
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Camara, Mamadou, Camara, Oumou, Ilboudo, Hamidou, Sakande, Hassan, Kaboré, Jacques, NʼDri, Louis, Jamonneau, Vincent, and Bucheton, Bruno
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- 2010
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35. PS-003: EVIDENCE-INFORMED POLICY MAKING: CHALLENGES AND OPPORTUNITIES
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Makanga, Michael, Beattie, Pauline, Breugelmans, Gabrielle, Nyirenda, Thomas, Bockarie, Moses, Tanner, Marcel, Volmink, Jimmy, Hankins, Catherine, Walzl, Gerhard, Chegou, Novel, Malherbe, Stephanus, Hatherill, Mark, Scriba, Thomas J., Zak, Daniel E., Barry, Clifton E., Kaufmann, Stefan H.E., Noor, Abdisalan, Strub-Wourgaft, Nathalie, Phillips, Patrick, Munguambe, Khátia, Ravinetto, Raffaella, Tinto, Halidou, Diro, Ermias, Mahendrahata, Yodi, Okebe, Joseph, Rijal, Suman, Garcia, Coralith, Sundar, Shyam, Ndayisaba, Gilles, Sopheak, Thai, Ngoduc, Thang, Loen, Harry Van, Jacobs, Jan, D'Alessandro, Umberto, Boelaert, Marleen, Buvé, Anne, Kamalo, Patrick, Manda-Taylor, Lucinda, Rennie, Stuart, Mokgatla, Boitumelo, Bahati, Prince, Ijsselmuiden, Carel, Afolabi, Muhammed, Mcgrath, Nuala, Kampmann, Beate, Imoukhuede, Egeruan, Alexander, Neal, Larson, Heidi, Chandramohan, Daniel, Bojang, Kalifa, Kasaro, Margaret Phiri, Muluka, Brenda, Kaunda, Kaunda, Morse, Jill, Westfall, Andrew, Kapata, Nathan, Kruuner, Annika, Henostroza, German, Reid, Stewart, Alabi, Abraham, Foguim, Francis, Sankarganesh, Jeyaraj, Bruske, Ellen, Mfoumbi, Arnault, Mevyann, Chester, Adegnika, Ayola, Lell, Bertrand, Kranzer, Katharina, Kremsner, Peter, Grobusch, Martin, Sabiiti, Wilber, Ntinginya, Nyanda, Kuchaka, Davis, Azam, Khalide, Kampira, Elizabeth, Mtafya, Bariki, Bowness, Ruth, Bhatt, Nilesh, Davies, Gerry, Kibiki, Gibson, Gillespie, Stephen, Lejon, Veerle, Ilboudo, Hamidou, Mumba, Dieudonné, Camara, Mamady, Kaba, Dramane, Lumbala, Crispin, Fèvre, Eric, Jamonneau, Vincent, Bucheton, Bruno, Büscher, Philippe, Chisenga, Caroline, Sinkala, Edford, Chilengi, Roma, Chitundu, Hellen, Zyambo, Zude, Wandeler, Gilles, Vinikoor, Michael, Emilie, Dama, Camara, Oumou, Mathurin, Koffi, Guiguigbaza-Kossigan, Dayo, Philippe, Büscher, Regassa, Fikru, Hassane, Sakande, Bienvenu, Somda Martin, Fabrice, Courtin, Ouédraogo, Elie, Kouakou, Lingue, Owusu, Michael, Mensah, Eric, Enimil, Anthony, Mutocheluh, Mohamed, Ndongo, Francis Ateba, Tejiokem, Mathurin Cyrille, Texier, Gaetan, Penda, Calixte, Ndiang, Suzie, Ndongo, Jean-Audrey, Guemkam, Georgette, Sofeu, Casimir Ledoux, Afumbom, Kfutwa, Faye, Albert, Msellati, Philippe, Warszawski, Josiane, Vos, Alinda, Devillé, Walter, Barth, Roos, Klipstein-Grobusch, Kerstin, Tempelman, Hugo, Venter, François, Coutinho, Roel, Grobbee, Diederick, Ssemwanga, Deogratius, Lyagoba, Frederick, Magambo, Brian, Kapaata, Anne, Kirangwa, Joseph, Nannyonjo, Maria, Nassolo, Faridah, Nsubuga, Rebecca, Yebra, Gonzalo, Brown, Andrew, Kaleebu, Pontiano, Nylén, Hanna, Habtewold, Abiy, Makonnen, Eyasu, Yimer, Getnet, Burhenne, Jürgen, Diczfalusy, Ulf, Aklillu, Eleni, Steele, Duncan, Walker, Richard, Simuyandi, Michelo, Beres, Laura, Bosomprah, Samuel, Ansumana, Rashid, Taitt, C., Lamin, J.M., Jacobsen, K.H., Mulvaney, S.P., Leski, T., Bangura, U., Stenger, D., Vries, Sophie De, Zinsou, Frejus Jeannot, Honkpehedji, J, Dejon, Jean Claude, Loembe, Marguerite Massinga, Bache, Bache, Pakker, Nadine, Leeuwen, Remko Van, Hounkpatin, Aurore Bouyoukou, Yazdanbakhsh, Maria, Bethony, Jeffrey, Hotez, Peter, Diemert, David, Bache, Bache Emmanuel, Fernandes, José F., Mba, Régis M Obiang, Kabwende, Anita L., Grobusch, Martin P., Krishna, Sanjeev, Kremsner, Peter G., Todagbe, Agnandji Selidji, Nambozi, Michael, Kabuya, Jean-Bertin, Hachizovu, Sebastian, Mwakazanga, David, Kasongo, Webster, Buyze, Jozefien, Mulenga, Modest, Geertruyden, Jean-Pierre, Gitaka, Jesse, Chan, Chim, Kongere, James, Kagaya, Wataru, Kaneko, Akira, Kabore, Naomie, Barry, Nouhoun, Kabre, Zachari, Werme, Karidia, Fofana, Aminata, Compaore, Daniel, Nikiema, Frederic, Some, Fabrice, Djimde, Abdoulaye, Zongo, Issaka, Ouedraogo, Bosco, Kone, Aminatou, Sagara, Issaka, Björkman, Anders, Gil, Jose Pedro, Nchinda, Godwin, Bopda, Alain, Nji, Nadesh, Ambada, Georgia, Ngu, Loveline, Tchadji, Jules, Sake, Carol, Magagoum, Suzanne, Njambe, Ghislain D., Lisom, Abel, Park, Chae Gyu, Tait, Dereck, Sibusiso, Hlatjwako, Manda, Olga, Croucher, Kristin, Westhuizen, Anja Van Der, Mshanga, Isaac, Levin, Jonathan, Nanvubya, Annet, Kibengo, Freddie, Jaoko, Walter, Pala, Pietro, Perreau, Matthieu, Namuniina, Annemarie, Kitandwe, Paul, Tapia, Gonzalo, Serwanga, Jennifer, Yates, Nicole, Fast, Pat, Mayer, Bryan, Montefiori, David, Tomaras, Georgia, Robb, Merlin, Lee, Carter, Wagner, Ralf, Sanders, Edward, Kilembe, William, Kiwanuka, Noah, Gilmour, Jill, Kuipers, Hester, Vooij, Dani, Chinyenze, Kundai, Priddy, Frances, Ding, Song, Hanke, Tom, Pantaleo, Giuseppe, Ngasala, Billy, Jovel, Irina, Malmberg, Maja, Mmbando, Bruno, Premji, Zul, Mårtensson, Andreas, Mwaiswelo, Richard, Agbor, Lenshina, Apinjoh, Tobias, Mwanza, Sydney, Chileshe, Justin, Joshi, Sudhaunshu, Malunga, Phidelis, Manyando, Christine, Laufer, Miriam, Dara, Antoine, Niangaly, Amadou, Sinha, Indranil, Brodin, David, Fofana, Bakary, Dama, Souleymane, Dembele, Demba, Sidibe, Bakary, Diallo, Nouhoum, Thera, Mahamadou, Wright, Karin, Gil, Jose, Doumbo, Ogobara, Baraka, Vito, Nabasumba, Carolyn, Francis, Filbert, Lutumba, Pascal, Mavoko, Hypolite, Alifrangis, Michael, Geertruyden, Jean-Pierre Van, Sissoko, Sekou, Sangaré, Cheick, Toure, Sekou, Sanogo, Kassim, Diakite, Hamadoun, Toure, Siaka, Doumbia, Diagassan, Haidara, Kadiatou, Julé, Amélie, Ashurst, Hazel, Merson, Laura, Olliaro, Piero, Marsh, Vicki, Lang, Trudie, Guérin, Philippe, Awuondo, Kennedy, Njenga, Daniel, Nyakarungu, Elizabeth, Titus, Pauline, Sutamihardja, Awalludin, Lowe, Brett, Ogutu, Bernhards, Billingsley, Peter, Soulama, Issiaka, Kaboré, Moïse, Coulibaly, Aboubacar, Ouattara, Maurice, Sanon, Souleymane, Diarra, Amidou, Bougouma, Edith, Ouedraogo, Alphonse, Sombie, Benjamin, Ouedraogo, Amidou, Kargougou, Désiré, Ouattara, Daouda, Issa, Nebie, Tiono, Alfred, Sirima, Sodiomon, Chaponda, Mike, Dabira, Edgard, Dao, François, Dara, Nianwalou, Sidibe, Bouran, Coulibaly, Moctar, Tolo, Allaye, Maiga, Hamma, Ouologuem, Nouhoum, Niangaly, Hamidou, Botchway, Felix, Wilson, Nana, Dickinson-Copeland, Carmen M, Adjei, Andrew A., Wilson, Michael, Stiles, Jonathan K., Hamid, Muzamil Abdel, Awad-Elgeid, Mona, Nasr, Awad, Netongo, Palmer, Kamdem, Séverin, Velavan, Thirumalaisamy, Lasry, Estrella, Diarra, Modibo, Bamadio, Amadou, Traore, Aliou, Coumare, Samba, Soma, Bahonan, Dicko, Yeyia, Sangare, Boubou, Tembely, Aly, Traore, Djibril, Haidara, Aboubecrin, Dicko, Alassane, Diawara, Elisabeth, Beavogui, Abdoul, Camara, Daouda, Sylla, Malick, Yattara, Mohamed, Sow, Amadou, Camara, Gnèpou Camara, Diallo, Saliou, Mombo-Ngoma, Ghyslain, Remppis, Jonathan, Sievers, Moritz, Manego, Rella Zoleko, Endamne, Lilian, Hutchinson, David, Held, Jana, Supan, Christian, Salazar, Carmen L. Ospina, Bonkian, Léa Nadège, Nahum, Alain, Sié, Ali, Abdulla, Salim, Cantalloube, Cathy, Djeriou, Elhadj, Bouyou-Akotet, Marielle, Mordmüller, Benjamin, Siribie, Mohamadou, Sirima, Sodiomon B., Ouattara, San Maurice, Coulibaly, Sam, Kabore, Jean Moïse, Amidou, Diarra, Tekete, Mamadou, Burhenne, Juergen, Traore, Oumar, Haefeli, Walter, Borrmann, Steffen, Kaboré, Naomie, Kabré, Zachari, Nikèma, Fréderic, Compaoré, Daniel, Somé, Fabrice, Djimdé, Abdoulaye, Ouédraogo, Jean, Chalwe, Victor, Miller, John, Diakité, Hamadoun, Greco, Beatrice, Spangenberg, Thomas, Kourany-Lefoll, Elly, Oeuvray, Claude, Mulry, Jim, Tyagarajan, Kamala, Magsaam, Bettina, Barnes, Karen, Hodel, Eva Maria, Humphreys, Georgina, Pace, Cheryl, Banda, C.G, Denti, Paulo, Allen, Elizabeth, Lalloo, David, Mwapasa, Victor, Terlouw, Anja, Mwesigwa, Julia, Achan, Jane, Jawara, Musa, Ditanna, Gian, Worwui, Archibald, Affara, Muna, Koukouikila-Koussounda, Félix, Kombo, Michael, Vouvoungui, Christevy, Ntoumi, Francine, Etoka-Beka, Mandingha Kosso, Deibert, Julia, Poulain, Pierre, Kobawila, Simon, Gueye, Nerly Gampio, Koukouikila-Koussounda, Felix, Seda, Brian, Kwambai, Titus, Jangu, Phelix, Samuels, Aaron, ter Kuile, Feike, Kariuki, Simon, Barry, Aissata, Bousema, Teun, Okech, Brenda, Egwang, Thomas, Corran, Patrick, Riley, Eleanor, Ezennia, Ifeoma, Ekwunife, Obinna, Muleba, Mbanga, Stevenson, Jennifer, Mbata, Keith, Coetzee, Maureen, Norris, Douglas, Moneke-Anyanwoke, Ngozi, Momodou, Jasseh, Clarke, Ed, Scott, Susana, Tijani, Adelani, Djimde, Moussa, Vaillant, Michel, Samouda, Hanen, Mensah, Victorine, Roetynck, Sophie, Kanteh, Ebrima, Bowyer, Georgina, Ndaw, Amy, Oko, Francis, Bliss, Carly, Jagne, Ya Jankey, Cortese, Riccardo, Nicosia, Alfredo, Roberts, Rachel, D'Alessio, Flavia, Leroy, Odile, Faye, Babacar, Cisse, Badara, Gerry, Stephen, Viebig, Nicola, Lawrie, Alison, Ewer, Katie, Hill, Adrian, Nebie, Issa, Tiono, Alfred B, Sanou, Guillaume, Konate, Amadou T, Yaro, Baptiste J, Sodiomon, Sirima, Honkpehedji, Yabo, Agobe, Jean Claude Dejon, Zinsou, Frejus, Mengue, Juliana, Richie, Thomas, Hoffman, Stephen, Nouatin, Odilon, Ngoa, Ulysse Ateba, Edoa, Jean R, Homoet, Andreas, Engelhon, Julie Englhon, Massinga-Louembe, Marguerite, Esen, Meral, Theisen, Michael, Sim, Kim Lee, Luty, Adrian Jf, Moutairou, Kabirou, Dinko, Bismarck, King, Elizabeth, Targett, Geoffrey, Sutherland, Colin, Likhovole, Clement, Ouma, Collins, Vulule, John, Musau, Susan, Khayumbi, Jeremiah, Okumu, Albert, Murithi, Wilfred, Otu, Jacob, Gehre, Florian, Zingue, Dezemon, Kudzawu, Samuel, Forson, Audrey, Mane, Morto, Rabna, Paulo, Diarra, Bassirou, Kayede, Salako, Adebiyi, Emmanuel, Kehinde, Aderemi, Onyejepu, Nneka, Onubogu, Catherine, Idigbe, Emmanuel, Ba, Awa, Diallo, Aissatou, Mboup, Souleymane, Disse, Kodjo, Kadanga, Gerard, Dagnra, Yaotse, Baldeh, Ignatius, Corrah, Tumani, Jong, Bouke De, Antonio, Martin, Musanabaganwa, Clarisse, Musabyimana, Jean Pierre, Karita, Etienne, Diop, Blondin, Nambajimana, Abidan, Dushimiyimana, Valentine, Karame, Prosper, Russell, Jim, Ndoli, Jules, Hategekimana, Theobald, Sendegeya, Augustin, Condo, Jeannine, Binagwaho, Agnes, Okonko, Iheanyi, Okerentugba, Phillip, Opaleye, Oluyinka, Awujo, Ezinwanne, Frank-Peterside, Nnenna, Moyo, Sikhulile, Kotokwe, Kenanao, Mohammed, Terence, Boleo, Coretah, Mupfumi, Lucy, Chishala, Samuel, Gaseitsiwe, Simani, Tsalaile, Lesedi, Bussmann, Herman, Makhema, Joseph, Baum, Marianna, Marlink, Richard, Engelbretch, Susan, Essex, Max, Novitsky, Vladimir, Saka, Emmanuel, Kalipalire, Zex, Bhairavabhotla, Ravikiran, Midiani, Dalitso, Sherman, Judith, Mgode, Georgies, Cox, Christophe, Bwana, Dickens, Mtui, Leah, Magesa, Daniel, Kahwa, Amos, Mfinanga, Godfrey, Mulder, Christiaan, Borain, Nick, Petersen, Lizette, Plessis, Julianne Du, Theron, Grant, Holm-Hansen, Carol, Tekwu, Emmanuel Mouafo, Sidze, Larissa Kamgue, Assam, Jean Paul Assam, Eyangoh, Sarah, Niemann, Stefan, Beng, Veronique Penlap, Frank, Matthias, Atiadeve, Samuel, Hilmann, Doris, Awoniyi, Dolapo, Baumann, Ralf, Kriel, Belinda, Jacobs, Ruschca, Kidd, Martin, Loxton, Andre, Kaempfer, Susanne, Singh, Mahavir, Mwanza, Winnie, Milimo, Deborah, Moyo, Maureen, Kasese, Nkatya, Cheeba-Lengwe, Maina, Munkondya, Stembiso, Ayles, Helen, Haas, Petra De, Muyoyeta, Monde, Namuganga, Anna Ritah, Kizza, Harriet Mayanja, Mendy, Alieu, Tientcheu, Leopold, Ayorinde, Abigail, Coker, Edward, Egere, Uzochukwu, Coussens, Anna, Naude, Celeste, Chaplin, George, Noursadeghi, Mahdad, Martineau, Adrian, Jablonski, Nina, Wilkinson, Robert, Ouedraogo, Henri Gautier, Matteelli, Alberto, Regazzi, Mario, Tarnagda, Grissoum, Villani, Paola, Sulis, Giorgia, Diagbouga, Serge, Roggi, Alberto, Giorgetti, Francesco, Kouanda, Seni, Bidias, Amel, Ndjonka, Dieudonné, Olemba, Clémence, Souleymanou, Arabo, Mukonzo, Jackson, Kuteesa, Ronald, Ogwal-Okeng, Jasper, Gustafsson, Lars L., Owen, Joel, Bassi, Peter, Gashau, Wadzani, Olaf, Klungel, Dodoo, Alexander, Okonkwo, Prosper, Kanki, Phyllis, Maruapula, Dorcas, Seraise, Boitumelo, Einkauf, Kevin, Reilly, Amanda, Rowley, Christopher, Musonda, Rosemary, Framhein, Anna, Mpagama, Stella, Semvua, Hadija, Maboko, Leonard, Hoelscher, Michael, Heinrich, Norbert, Mulenga, Lloyd, Kaayunga, Callistus, Davies, Mary-Ann, Egger, Matthias, Musukuma, Kalo, Dambe, Rosalia, Usadi, Benjamin, Ngari, Moses, Thitiri, Johnstone, Mwalekwa, Laura, Fegan, Greg, Berkley, James, Nsagha, Dickson, Munamunungu, Virginia, Bolton, Carolyn, Siyunda, Alice, Shilimi, Jacinta, Bucciardini, Raffaella, Fragola, Vincenzo, Abegaz, Teshome, Lucattini, Stefano, Halifom, Atakilt, Tadesse, Eskedar, Berhe, Micheal, Pugliese, Katherina, Castro, Paola De, Terlizzi, Roberta, Fucili, Luca, Gregorio, Massimiliano Di, Mirra, Marco, Zegeye, Teame, Binelli, Andrea, Vella, Stefano, Abraham, Loko, Godefay, Hagos, Rakotoarivelo, Rivo, Raberahona, Mihaja, Randriamampionona, Njary, Andriamihaja, Rabezanahary, Rasamoelina, Tahinamandranto, Cornet, Muriel, Randria, Mamy Jean De Dieu, Benet, Thomas, Vanhems, Philippe, Andrianarivelo, Mala Rakoto, Chirwa, Uchizi, Michelo, Charles, Hamoonga, Raymond, Wandiga, Steve, Oduor, Patience, Agaya, Janet, Sharma, Aditya, Cavanaugh, Sean, Cain, Kevin, Mukisa, John, Mupere, Ezekiel, Worodria, William, Ngom, Justice Trésor, Koro, Francioli, Godwe, Celestin, Adande, Clemence, Ateugieu, Romaric, Onana, Tatiana, Ngono, Annie, Kamdem, Yannick, Ngo-Niobe, Sara, Etoa, François-Xavier, Kanengoni, Muchineripi, Ruzario, Sithembile, Ndebele, Paul, Shana, Melody, Tarumbiswa, Fadzai, Musesengwa, Rosemary, Gutsire, Rutendo, Fisher, Kevin, Thyagarajan, Bargavi, Akanbi, Olusola, Binuyo, Michael, Ssengooba, Willy, Respeito, Durval, Mambuque, Edson, Blanco, Silvia, Mandomando, Inacio, Cobelens, Frank, Garcia-Basteiro, Alberto, Tamene, Ayele, Topp, Stephanie, Mwamba, Chanda, Padian, Nancy, Sikazwe, Izukanji, Geng, Elvin, Holmes, Charles, Sikombe, Kombatende, Hantuba, Cardinal, Czaicki, Nancy, Simbeza, Sandra, Somwe, Paul, Umulisa, Michele, Ilo, Jennifer, Kestelyn, Evelyne, Uwineza, Mireille, Agaba, Stephen, Delvaux, Therese, Wijgert, Janneke, Gethi, Dickson, Odeny, Lazarus, Tamandjou, Cynthia, Kaindjee-Tjituka, Francina, Brandt, Laura, Cotton, Mark, Nel, Etienne, Preiser, Wolfgang, Andersson, Monique, Adepoju, Abiola, Magana, Musa, Etsetowaghan, Andrew, Chilikwazi, Mutinta, Sutcliffe, Catherine, Thuma, Philip, Sinywimaanzi, Kathy, Matakala, Hellen, Munachoonga, Passwell, Moss, William, Masenza, Issa Sabi, Geisenberger, Otto, Agrea, Peter, Rwegoshora, France, Mahiga, Hellen, Olomi, Willyhelmina, Kroidl, Arne, Kayode, Gbenga, Amoakoh-Coleman, Mary, Ansah, Evelyn, Uthman, Olalekan, Fokam, Joseph, Santoro, Maria-Mercedes, Musolo, Chrissie, Chimbiri, Isabel, Chikwenga, Gloria, Deula, Ruth, Massari, Riccardo, Lungu, Agness, Perno, Carlo-Federico, Ndzengue, Georgia, Loveline, Ngu, Lissom, Abel, Flaurent, Tchouangueu, Sosso, Samuel, Essomba, Claudine, Kpeli, Grace, Otchere, Isaac, Lamelas, Araceli, Buultjens, Andrew, Bulach, Dieter, Baines, Sarah, Seemann, Torsten, Giulieri, Stefano, Nakobu, Zuliehatu, Aboagye, Samuel, Owusu-Mireku, Evelyn, Danso, Emelia, Hauser, Julia, Hinic, Vladimira, Pluschke, Gerd, Stinear, Timothy, Yeboah-Manu, Dorothy, Elshayeb, Ayman, Siddig, Marmar El, Ahmed, Abdel Azim, Hussien, Adil El, Kabwe, Mwila, Tembo, John, Chilukutu, Lophina, Chilufya, Moses, Ngulube, Francis, Lukwesa, Chileshe, Enne, Virve, Wexner, Hannah, Mwananyanda, Lawrence, Hamer, Davidson, Sinyangwe, Sylvester, Ahmed, Yusuf, Klein, Nigel, Maeurer, Markus, Zumla, Ali, Bates, Matthew, Beyala, Landry, Etienne, Guenou, Anthony, Njimbia, Benjamin, Azike, Ateudjieu, Jerome, Chibwe, Bertha, Ojok, David, Tarr, Christine Attia, Perez, Guillermo Martinez, Omeonga, Senga, Kibungu, Fanta, Meyer, Ana, Lansana, Peter, Mayor, Alfredo, Onyango, Peter, Loggerenberg, François Van, Furtado, Tamzin, Boggs, Liam, Segrt, Alexis, Dochez, Carine, Burnett, Rosemary, Mphahlele, M. Jeffrey, Miiro, George, Mbidde, Edward, Peshu, Norbert, Kivaya, Esther, Ngowi, Bernard, Kavishe, Reginald, Maowia, Mukhtar, Sandstrom, Eric, Ayuo, Elizabeth, Mmbaga, Blandina, Leisegang, Cordelia, Thorpe, Marie, Batchilly, Elizabeth, N'Guessan, Jean-Pierre, Kanteh, Dembo, Søfteland, Solrun, Sebitloane, Motshedisi, Vwalika, Bellington, Taylor, Myra, Galappaththi-Arachchige, Hashini, Holmen, Sigve, Gundersen, Svein Gunnar, Ndhlovu, Patricia, Kjetland, Eyrun Floerecke, Kombe, Francis, Toohey, Jacintha, Pienaar, Elizabeth, Kredo, Tamara, Cham, Pa Modou, Abubakar, Ismaela, Dondeh, Bai Lamin, Vischer, Nerina, Pfeiffer, Constanze, Burri, Christian, Musukwa, Kalo, Zürcher, Samuel, Mwandu, Temwani, Bauer, Sophie, Adriko, Moses, Mwaura, Peter, Omolloh, Kevin, Jones, Clarer, Malecela, Mwelecele, Hamidu, Buhari Adamu, Jenner, Tettevi Edward, Asiedu, Larbi John, Osei-Atweneboana, Mike, Afeke, Innocent, Addo, Phyllis, Newman, Mercy, Durnez, Lies, Eddyani, Miriam, Ammisah, Nana, Abas, Mona, Quartey, Maxwell, Ablordey, Anthony, Akinwale, Olaoluwa, Adeneye, Adeniyi, Ezeugwu, Sylvanus, Olukosi, Yetunde, Adewale, Babatunde, Sulyman, Medinat, Mafe, Margaret, Okwuzu, Jane, Gyang, Pam, Nwafor, Timothy, Henry, Uzoma, Musa, Bilkisu, Ujah, Innocent, Agobé, Jean Claude Dejon, Grau-Pujol, Berta, Sacoor, Charfudin, Nhabomba, Augusto, Casellas, Aina, Quintó, Llorenç, Subirà, Carme, Giné, Ricard, Valentín, Antònia, Muñoz, Jose, Nikiema, Marguerite, Ky-Ba, Absatou, Comapore, Kiswendsida Abdou Muller, Traore, Alfred, Sangare, Lassana, Oluremi, Adeolu, Michel, Mandro, Camara, Yaya, Sanneh, Bakary, Cuamba, Inocencia, Gutiérrez, Jose, Lázaro, Carlota, Mejia, Rojelio, Adedeji, Abimbola, Folorunsho, Sola, Demehin, Pelumi, Akinsanya, Bamidele, Cowley, Giovanna, Silva, Eunice Teixeira Da, Nabicassa, Meno, Barros, Pedrozinho Duarte Pereira De, Blif, Milena Mbote, Bailey, Robin, Last, Anna, Mahendradhata, Yodi, Gotuzzo, Eduardo, Nys, Kateljine De, Casteels, Minnes, Nona, Sylvie Kwedi, Lumeka, Kabwende, Todagbe, Agnandji, Djima, Mariam Mama, Ukpong, Morenike, Sagay, Atiene, Khamofu, Hadiza, Torpey, Kwasi, Afiadigwe, Evaristus, Anenih, James, Ezechi, Oliver, Nweneka, Chidi, Idoko, John, Muhumuza, Simon, Katahoire, Anne, Nuwaha, Fred, Olsen, Annette, Okeyo, Seth, Omollo, Raymond, Kimutai, Robert, Ochieng, Michael, Egondi, Thaddaeus, Moonga, Clement, Chileshe, Chisele, Magwende, George, Anumudu, Chiaka, Onile, Olugbenga, Oladele, Victoria, Adebayo, Adewale, Awobode, Henrietta, Oyeyemi, Oyetunde, Odaibo, Alexander, Kabuye, Emily, Lutalo, Tom, Njua-Yafi, Clarisse, Nkuo-Akenji, Theresa, Anchang-Kimbi, Judith, Mugri, Regina, Chi, Hanesh, Tata, Rolland, Njumkeng, Charles, Dodoo, Daniel, Achidi, Eric, Fernandes, José, Bache, Emmanuel B., Matakala, Kalumbu, Searle, Kelly, Greenman, Michelle, and Rainwater-Lovett, Kaitlin
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Abstracts of Poster Presentations ,Abstracts of Oral Presentations ,Author Index ,Abstracts of Presentations in Plenary Sessions ,Article ,Abstracts of the Eighth Edctp Forum, 6–9 November 2016 - Published
- 2017
36. Macrophage migrating inhibitory factor expression is associated with Trypanosoma brucei gambiense infection and is controlled by trans-acting expression quantitative trait loci in the Guinean population
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Kabore, Justin Windingoudi, Camara, Oumou, Ilboudo, Hamidou, Capewell, Paul, Clucas, Caroline, Cooper, Anneli, Kabore, Jacques, Camara, Mamadou, Jamonneau, Vincent, Hertz-Fowler, Christiana, Belem, Adrien Marie Gaston, Matovu, Enock, Macleod, Annette, Sidibe, Issa, Noyes, Harry, Bucheton, Bruno, Grp, TrypanoGEN Res, Consortium, H3Africa, Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université de Bordeaux (UB)
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Adult ,Male ,0301 basic medicine ,Microbiology (medical) ,Chemokine ,Adolescent ,Trypanosoma brucei gambiense ,medicine.medical_treatment ,[SDV]Life Sciences [q-bio] ,Quantitative Trait Loci ,030106 microbiology ,Population ,Microbiology ,Young Adult ,03 medical and health sciences ,Genotype ,Genetics ,medicine ,Humans ,CXCL13 ,Child ,education ,Macrophage Migration-Inhibitory Factors ,Molecular Biology ,Gene ,Ecology, Evolution, Behavior and Systematics ,Aged ,Aged, 80 and over ,education.field_of_study ,biology ,Gene Expression Profiling ,Middle Aged ,Acquired immune system ,Chemokine CXCL13 ,3. Good health ,Trypanosomiasis, African ,030104 developmental biology ,Infectious Diseases ,Cytokine ,Gene Expression Regulation ,Child, Preschool ,Immunology ,Expression quantitative trait loci ,biology.protein ,Female ,Guinea - Abstract
Infection by Trypanosoma brucei gambiense is characterized by a wide array of clinical outcomes, ranging from asymptomatic to acute disease and even spontaneous cure. In this study, we investigated the association between macrophage migrating inhibitory factor (MIF), an important pro-inflammatory cytokine that plays a central role in both innate and acquired immunity, and disease outcome during T. b. gambiense infection. A comparative expression analysis of patients, individuals with latent infection and controls found that MIF had significantly higher expression in patients (n = 141; 1.25 ± 0.07; p
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- 2019
37. Do cryptic reservoirs threaten gambiense-sleeping sickness elimination?
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Informal Expert Group on Gambiense HAT Reservoirs, Büscher, Philippe, Bart, Jean-Mathieu, Boelaert, Marleen, Bucheton, Bruno, Cecchi, Giuliano, Chitnis, Nakul, Courtin, David, Figueiredo, Luisa M., Franco, José-Ramon, Grébaut, Pascal, Hasker, Epco, Ilboudo, Hamidou, Jamonneau, Vincent, Koffi, Mathurin, Lejon, Veerle, MacLeod, Annette, Masumu, Justin, Matovu, Enock, Mattioli, Raffaele, Noyes, Harry, Picado, Albert, Rock, Kat S., Rotureau, Brice, Simo, Gustave, Thévenon, Sophie, Trindade, Sandra, Truc, Philippe, and Van Reet, Nick
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- 2018
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38. PO 8441 EXPERIMENTAL COMPARISON OF SENSITIVITY OF LAMP AND REAL-TIME PCR
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Kaboré, Jacques, primary, Ilboudo, Hamidou, additional, Compaoré, Charlie FA, additional, Camara, Oumou, additional, Bamba, Mohamed, additional, Sakandé, Hassane, additional, Camara, Mamadou, additional, Bucheton, Bruno, additional, Kaba, Dramane, additional, Jamonneau, Vincent, additional, Deborggraeve, Stijn, additional, and Lejon, Veerle, additional
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- 2019
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39. PO 8473 IMPACT OF EBOLA ON SLEEPING SICKNESS IN COASTAL GUINEA: A RETROSPECTIVE ANALYSIS (2012–2017) FROM THE GUINEAN NATIONAL CONTROL PROGRAMME
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Camara, Oumou, primary, Ilboudo, Hamidou, additional, Camara, Mariame, additional, Ouattara, Eric, additional, Duvignaud, Alexandre, additional, Leno, Amadou, additional, Solano, Philippe, additional, Malvy, Denis, additional, Bucheton, Bruno, additional, and Camara, Mamadou, additional
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- 2019
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40. A polymorphism in the haptoglobin, haptoglobin related protein locus is associated with risk of human sleeping sickness within Cameroonian populations
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Ofon, Elvis, Noyes, Harry, Mulindwa, Julius, Ilboudo, Hamidou, Simuunza, Martin, Eboo, Vincent, Njiokou, Flobert, Koffi, Mathurin, Bucheton, Bruno, Fogue, Pythagore, Hertz-Fowler, Christiane, MacLeod, Annette, Simo, Gustave, Grp, TrypanoGEN Res, and Consortium, H3Africa
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Male ,0301 basic medicine ,Heredity ,Trypanosoma brucei gambiense ,Biochemistry ,Geographical Locations ,Gene Frequency ,Risk Factors ,Zoonoses ,Medicine and Health Sciences ,Cameroon ,Child ,Aged, 80 and over ,Protozoans ,Genetics ,biology ,lcsh:Public aspects of medicine ,Haptoglobin ,Neglected Diseases ,Eukaryota ,Middle Aged ,3. Good health ,Genetic Mapping ,Infectious Diseases ,Female ,Research Article ,Neglected Tropical Diseases ,Adult ,Trypanosoma ,Genotyping ,medicine.medical_specialty ,lcsh:Arctic medicine. Tropical medicine ,Adolescent ,Genotype ,lcsh:RC955-962 ,Variant Genotypes ,Locus (genetics) ,Single-nucleotide polymorphism ,Research and Analysis Methods ,Polymorphism, Single Nucleotide ,African Trypanosomiasis ,Molecular Genetics ,Young Adult ,03 medical and health sciences ,Antigens, Neoplasm ,Trypanosomiasis ,Molecular genetics ,Parasitic Diseases ,medicine ,Humans ,SNP ,Genetic Predisposition to Disease ,Molecular Biology Techniques ,Molecular Biology ,Alleles ,Genetic Association Studies ,Aged ,Protozoan Infections ,Plasma Proteins ,Haptoglobins ,Organisms ,Public Health, Environmental and Occupational Health ,Biology and Life Sciences ,Proteins ,lcsh:RA1-1270 ,Tropical Diseases ,Parasitic Protozoans ,Minor allele frequency ,Trypanosomiasis, African ,030104 developmental biology ,Genetic Loci ,Case-Control Studies ,Asymptomatic Diseases ,People and Places ,Africa ,Immunology ,biology.protein ,Asymptomatic carrier - Abstract
Background Human African Trypanosomiasis (HAT) is a neglected disease targeted for elimination as a public health problem by 2020. Elimination requires a better understanding of the epidemiology and clinical evolution of HAT. In addition to the classical clinical evolution of HAT, asymptomatic carriers and spontaneous cure have been reported in West Africa. A genetic component to human susceptibility to HAT has been suggested to explain these newly observed responses to infection. In order to test for genetic associations with infection response, genetic polymorphism in 17 genes were tested (APOL1, IL1B, IL4, IL4R, IL6, IL8, IL12B, IL12RB1, IL10, TNFA, INFG, MIF, HLA-G, HLA-A, HP, HPR and CFH). Methodology A case-control study was performed on 180 blood samples collected from 56 cases and 124 controls from Cameroon. DNA was extracted from blood samples. After quality control, 25 samples (24 controls and 1 case) were eliminated. The genotyping undertaken on 155 individuals including 55 cases and 100 controls were investigated at 96 loci (88 SNPs and 8 indels) located on 17 genes. Associations between these loci and HAT were estimated via a case-control association test. Results Analyses of 64 SNPs and 4 indels out of 96 identified in the selected genes reveal that the minor allele (T) of rs8062041 in haptoglobin (HP) appeared to be protective against HAT (p = 0.0002395, OR 0.359 (CI95 [0.204–0.6319])); indicating higher frequency in cases compared to controls. This minor allele with adjusted p value of 0.0163 is associated with a lower risk (protective effect) of developing sleeping sickness. Conclusion The haptoglobin related protein HPR and HP are tightly linked and both are duplicated in some people and may lead to higher activity. This increased production could be responsible of the protection associated with rs8062041 even though this SNP is within HP., Author summary Human African trypanosomiasis (HAT) or sleeping sickness is a neglected tropical disease targeted for elimination by 2020. This elimination requires a better understanding of the epidemiology and clinical evolution of this disease. Beside the classical clinical evolution, asymptomatic carriers, seropositive and spontaneous cure of infected persons have been reported in West Africa. Arguments in favor of human genetic susceptibility to HAT have been raised to explain this variability in clinical presentation. This study investigated the genetic polymorphism of 17 genes between controls and sleeping sickness patients in Southern Cameroon in order to improve our knowledge of human susceptibility to trypanosome infections. We identified single nucleotide polymorphisms and indels in 17 selected genes involved in immune responses and carried out a case-control candidate gene association study and demonstrated differences between variants associated with the disease. From these genes, only haptoglobin (HP) at the SNP rs8062041 was found to have polymorphisms which were strongly associated with trypanosomiasis. The minor allele (T) at this SNP position appeared to be protective against HAT (p = 0.0002395, OR 0.359 (CI95 [0.204–0.6319])) reducing the risk of developing disease approximately threefold. The haptoglobin related protein (HPR) is adjacent to HP and is a component of the Trypanolytic factor that kills trypanosomes. The HP and HPR locus is duplicated in some people. The rs8062041 variant may be associated with this duplication and it is possible that increased production of HPR is the cause of the protection associated with rs8062041. The results reported here will contribute to the knowledge of the role of human genetics in disease progression, and thus lead to the identification of novel biomarkers which could involve development of new diagnostics, treatments and intervention strategies.
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- 2017
41. Introducing the TrypanoGEN biobank: A valuable resource for the elimination of human African trypanosomiasis
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Ilboudo, Hamidou, Noyes, Harry, Mulindwa, Julius, Kimuda, Magambo Phillip, Koffi, Mathurin, Kabore, Justin Windingoudi, Ahouty, Bernadin, Ngoyi, Dieudonne Mumba, Fataki, Olivier, Simo, Gustave, Ofon, Elvis, Enyaru, John, Chisi, John, Kamoto, Kelita, Simuunza, Martin, Alibu, Vincent P, Lejon, Veerle, Jamonneau, Vincent, Macleod, Annette, Camara, Mamadou, Bucheton, Bruno, Hertz-Fowler, Christiane, Sidibe, Issa, Matovu, Enock, Grp, TrypanoGEN Res, and Consortium, H3Africa
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0301 basic medicine ,Aging ,Geographical Locations ,Plasma ,Environmental protection ,Zoonoses ,Medicine and Health Sciences ,African trypanosomiasis ,Uganda ,Cameroon ,Biological Specimen Banks ,Aged, 80 and over ,lcsh:Public aspects of medicine ,Genomics ,Middle Aged ,Biobank ,Infectious Diseases ,Neglected Tropical Diseases ,Adult ,Resource (biology) ,lcsh:Arctic medicine. Tropical medicine ,Adolescent ,lcsh:RC955-962 ,Zambia ,Biology ,African Trypanosomiasis ,03 medical and health sciences ,Young Adult ,Trypanosomiasis ,medicine ,Parasitic Diseases ,Genome-Wide Association Studies ,Genetics ,Humans ,Genetic Predisposition to Disease ,Disease Eradication ,Environmental planning ,Africa South of the Sahara ,Aged ,Symposium ,Protozoan Infections ,Public Health, Environmental and Occupational Health ,Biology and Life Sciences ,Computational Biology ,Human Genetics ,lcsh:RA1-1270 ,DNA ,medicine.disease ,Tropical Diseases ,Genome Analysis ,030104 developmental biology ,Trypanosomiasis, African ,People and Places ,Africa ,Parasitology - Abstract
No abstract available.
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- 2017
42. The study of trypanosome species circulating in domestic animals in two human African trypanosomiasis foci of Cote d'Ivoire identifies pigs and cattle as potential reservoirs of Trypanosoma brucei gambiense
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N’Djetchi, Martial Kassi, Ilboudo, Hamidou, Koffi, Mathurin, Kaboré, Jacques, Kaboré, Justin Windingoudi, Kaba, Dramane, Courtin, Fabrice, Coulibaly, Bamoro, Fauret, Pierre, Kouakou, Lingué, Ravel, Sophie, Deborggraeve, Stijn, Solano, Philippe, De Meeûs, Thierry, Bucheton, Bruno, and Jamonneau, Vincent
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Swine Diseases ,lcsh:Arctic medicine. Tropical medicine ,lcsh:RC955-962 ,Swine ,lcsh:Public aspects of medicine ,Trypanosoma brucei gambiense ,Cattle Diseases ,lcsh:RA1-1270 ,Cote d'Ivoire ,Trypanosomiasis, African ,parasitic diseases ,Animals ,Humans ,Cattle ,Research Article ,Disease Reservoirs - Abstract
Background Important control efforts have led to a significant reduction of the prevalence of human African trypanosomiasis (HAT) in Côte d’Ivoire, but the disease is still present in several foci. The existence of an animal reservoir of Trypanosoma brucei gambiense may explain disease persistence in these foci where animal breeding is an important source of income but where the prevalence of animal African trypanosomiasis (AAT) is unknown. The aim of this study was to identify the trypanosome species circulating in domestic animals in both Bonon and Sinfra HAT endemic foci. Methodology/Principal findings 552 domestic animals (goats, pigs, cattle and sheep) were included. Blood samples were tested for trypanosomes by microscopic observation, species-specific PCR for T. brucei sl, T. congolense, T. vivax and subspecies-specific PCR for T. b. gambiense and T. b. gambiense immune trypanolysis (TL). Infection rates varied significantly between animal species and were by far the highest in pigs (30%). T. brucei s.l was the most prevalent trypanosome species (13.7%) followed by T. congolense. No T. b. gambiense was identified by PCR while high TL positivity rates were observed using T. b. gambiense specific variants (up to 27.6% for pigs in the Bonon focus). Conclusion This study shows that domestic animals are highly infected by trypanosomes in the studied foci. This was particularly true for pigs, possibly due to a higher exposure of these animals to tsetse flies. Whereas T. brucei s.l. was the most prevalent species, discordant results were obtained between PCR and TL regarding T. b. gambiense identification. It is therefore crucial to develop better tools to study the epidemiological role of potential animal reservoir for T. b. gambiense. Our study illustrates the importance of “one health” approaches to reach HAT elimination and contribute to AAT control in the studied foci., Author summary In Africa, significant efforts to control human African trypanosomiasis (HAT) over the past three decades have drastically reduced the prevalence of the disease and elimination seems today an achievable goal. However, potential animal reservoirs of Trypanosoma brucei gambiense may compromise this ambitious objective. In the Bonon and Sinfra HAT endemic foci in Côte d’Ivoire, no recent data are available about the prevalence of animal African trypanosomiasis (AAT). The aim of this study was to identify trypanosomes circulating in domestic animals in these two HAT foci using serological, parasitological and molecular tools. We showed that T. brucei s.l. and T. congolense were the most prevalent trypanosome species and that pigs and cattle were the most infected animals. Discordant results were observed between the T. b. gambiense specific molecular and serological tools and the presence of an animal reservoir for T. b. gambiense remains unclear. Nevertheless, improved control strategies can be proposed based on this study to reach HAT elimination and contribute to AAT control in the study areas.
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- 2017
43. Copy number variation in human genomes from three major ethno-linguistic groups in Africa.
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Nyangiri, Oscar A., Noyes, Harry, Mulindwa, Julius, Ilboudo, Hamidou, Kabore, Justin Windingoudi, Ahouty, Bernardin, Koffi, Mathurin, Asina, Olivier Fataki, Mumba, Dieudonne, Ofon, Elvis, Simo, Gustave, Kimuda, Magambo Phillip, Enyaru, John, Alibu, Vincent Pius, Kamoto, Kelita, Chisi, John, Simuunza, Martin, Camara, Mamadou, Sidibe, Issa, and MacLeod, Annette
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GENOMES ,DNA copy number variations ,AFRICANS ,DISEASE susceptibility ,HUMAN genome ,HAPLOTYPES - Abstract
Background: Copy number variation is an important class of genomic variation that has been reported in 75% of the human genome. However, it is underreported in African populations. Copy number variants (CNVs) could have important impacts on disease susceptibility and environmental adaptation. To describe CNVs and their possible impacts in Africans, we sequenced genomes of 232 individuals from three major African ethno-linguistic groups: (1) Niger Congo A from Guinea and Côte d'Ivoire, (2) Niger Congo B from Uganda and the Democratic Republic of Congo and (3) Nilo-Saharans from Uganda. We used GenomeSTRiP and cn.MOPS to identify copy number variant regions (CNVRs). Results: We detected 7608 CNVRs, of which 2172 were only deletions, 2384 were only insertions and 3052 had both. We detected 224 previously un-described CNVRs. The majority of novel CNVRs were present at low frequency and were not shared between populations. We tested for evidence of selection associated with CNVs and also for population structure. Signatures of selection identified previously, using SNPs from the same populations, were overrepresented in CNVRs. When CNVs were tagged with SNP haplotypes to identify SNPs that could predict the presence of CNVs, we identified haplotypes tagging 3096 CNVRs, 372 CNVRs had SNPs with evidence of selection (iHS > 3) and 222 CNVRs had both. This was more than expected (p < 0.0001) and included loci where CNVs have previously been associated with HIV, Rhesus D and preeclampsia. When integrated with 1000 Genomes CNV data, we replicated their observation of population stratification by continent but no clustering by populations within Africa, despite inclusion of Nilo-Saharans and Niger-Congo populations within our dataset. Conclusions: Novel CNVRs in the current study increase representation of African diversity in the database of genomic variants. Over-representation of CNVRs in SNP signatures of selection and an excess of SNPs that both tag CNVs and are subject to selection show that CNVs may be the actual targets of selection at some loci. However, unlike SNPs, CNVs alone do not resolve African ethno-linguistic groups. Tag haplotypes for CNVs identified may be useful in predicting African CNVs in future studies where only SNP data is available. [ABSTRACT FROM AUTHOR]
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- 2020
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44. Trypanosome-induced Interferon-γ production in whole blood stimulation assays is associated with latent Trypanosoma brucei gambiense infections
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Ilboudo, Hamidou, Jamonneau, Vincent, Koffi, Mathurin, Kaboré, Jacques, Amoussa, Roukiyath, Holzmuller, Philippe, Garcia, André, Bucheton, Bruno, and Courtin, David
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- 2016
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45. Differences in pathogenicity and virulence of Trypanosoma brucei gambiense field isolates in experimentally infected Balb/C mice
- Author
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Kaboré, Jacques, primary, Camara, Oumou, additional, Koffi, Mathurin, additional, Sanou, Djénéba, additional, Ilboudo, Hamidou, additional, Sakandé, Hassane, additional, Camara, Mamadou, additional, De Meeûs, Thierry, additional, Ravel, Sophie, additional, Belem, Adrien Marie Gaston, additional, MacLeod, Annette, additional, Bucheton, Bruno, additional, Jamonneau, Vincent, additional, and Thévenon, Sophie, additional
- Published
- 2018
- Full Text
- View/download PDF
46. Description of the first sleeping sickness case diagnosed in Burkina Faso since two decades
- Author
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Dama, Emilie, primary, Drabo, Aboubacar, additional, Kaboré, Jacques, additional, Ouédraogo, Elie, additional, Coulibaly, Bamoro, additional, Ilboudo, Hamidou, additional, Kaboré, Justin, additional, Compaoré, Charlie Franck, additional, Sakandé, Hassane, additional, Ouédraogo, Micheline, additional, Rayaissé, Jean-Baptiste, additional, Courtin, Fabrice, additional, Solano, Philippe, additional, Drabo, François, additional, and Jamonneau, Vincent, additional
- Published
- 2018
- Full Text
- View/download PDF
47. Ebola outbreak brings to light an unforeseen impact of tsetse control on sleeping sickness transmission in Guinea
- Author
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Kagabadouno, Moïse, primary, Camara, Oumou, additional, Camara, Mamadou, additional, Ilboudo, Hamidou, additional, Camara, Mariame, additional, Rayaisse, Jean-Baptiste, additional, Diaby, Abdoulaye, additional, Traoré, Balla, additional, Leno, Mamadou, additional, Courtin, Fabrice, additional, Jamonneau, Vincent, additional, Solano, Philippe, additional, and Bucheton, Bruno, additional
- Published
- 2018
- Full Text
- View/download PDF
48. The study of trypanosome species circulating in domestic animals in two human African trypanosomiasis foci of Côte d'Ivoire identifies pigs and cattle as potential reservoirs of Trypanosoma brucei gambiense
- Author
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N’Djetchi, Martial Kassi, primary, Ilboudo, Hamidou, additional, Koffi, Mathurin, additional, Kaboré, Jacques, additional, Kaboré, Justin Windingoudi, additional, Kaba, Dramane, additional, Courtin, Fabrice, additional, Coulibaly, Bamoro, additional, Fauret, Pierre, additional, Kouakou, Lingué, additional, Ravel, Sophie, additional, Deborggraeve, Stijn, additional, Solano, Philippe, additional, De Meeûs, Thierry, additional, Bucheton, Bruno, additional, and Jamonneau, Vincent, additional
- Published
- 2017
- Full Text
- View/download PDF
49. T cell activation and the HLA locus associate with latent infections of human African trypanosomiasis
- Author
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Capewell, Paul, primary, Bucheton, Bruno, additional, Clucas, Caroline, additional, Ilboudo, Hamidou, additional, Cooper, Anneli, additional, Gorman, Taylor-Anne, additional, O’Neill, Kerry, additional, Patakas, Agapitos, additional, Platt, Andrew, additional, Vaikkinen, Heli, additional, Weir, William, additional, Camara, Mamadou, additional, Garside, Paul, additional, Jamonneau, Vincent, additional, and MacLeod, Annette, additional
- Published
- 2017
- Full Text
- View/download PDF
50. APOL1 renal risk variants have contrasting resistance and susceptibility associations with African trypanosomiasis
- Author
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Cooper, Anneli, primary, Ilboudo, Hamidou, additional, Alibu, V Pius, additional, Ravel, Sophie, additional, Enyaru, John, additional, Weir, William, additional, Noyes, Harry, additional, Capewell, Paul, additional, Camara, Mamadou, additional, Milet, Jacqueline, additional, Jamonneau, Vincent, additional, Camara, Oumou, additional, Matovu, Enock, additional, Bucheton, Bruno, additional, and MacLeod, Annette, additional
- Published
- 2017
- Full Text
- View/download PDF
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