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Your search keyword '"Imad M. Awan"' showing total 2 results
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2 results on '"Imad M. Awan"'

1. List of Contributors

Author

Enrique Amaya, Imad M. Awan, Hugo J. Bellen, Alessandro Bertero, David Brindley, Stephanie Brown, Eleanor Jane Budge, Eleni Chantzoura, John Collin, Amanda S. Coutts, Karel Dorey, Jefte M. Drijvers, Tomasz Gwozdz, Gregory A. Hawkins, Shoko Ishibashi, Tyler Jacks, Mehdi Jalali, Morteza Jalali, Sonali Joshi, Keisuke Kaji, Nicholas B. La Thangue, Laurens J. Lambert, Robert Lea, Martyna Lukoseviciute, Paul Martin, Mandar D. Muzumdar, Frederick D. Park, Cory A. Perugino, Adam Pettitt, David Pettitt, Shiv Pillai, Tannishtha Reya, William M. Rideout, MacKenna Roberts, Ian M. Rosenberg, Francesca Y.L. Saldanha, Roman Sasik, Charis-P. Segeritz, Semira Sheikh, James Smith, Ludovic Vallier, Michael F. Wangler, Dihua Yu, and Justyna Zaborowska

Published
2017
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2. The Enzyme-Linked Immunosorbent Assay

Author

Jefte M. Drijvers, Cory A. Perugino, Imad M. Awan, Shiv Pillai, and Ian Rosenberg

Subjects
0301 basic medicine, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Computer science, Research context, Immunology, Assay, Elisa assay, Computational biology, Antigen measurement, 030215 immunology, Antibody detection
Abstract

The enzyme-linked immunosorbent assay (ELISA) has evolved from other types of immunoassays in the early 1970s and is now one of the most widely used laboratory techniques in clinical, translational, and basic sciences as well as clinical medicine. In this chapter, the basic concepts of ELISA and its most commonly used forms, such as sandwich, indirect, and competitive assays, are discussed, as well as their applications and the considerations involved in designing a new ELISA assay. The focus of this chapter is on the use of ELISA in clinical and translational research, aiming to provide the reader with the necessary tools to apply ELISA in their own research context. An example of how ELISA can be employed in a translational research scenario is provided, as well as a detailed protocol describing how to do so in that example.

Published
2017
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