1. Transcriptome analysis reveals that gga-miR-2954 inhibits the inflammatory response against Eimeria tenella infection.
- Author
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Yu H, Tang J, Dong L, Tang M, Arif A, Zhang T, Zhang G, Xie K, Zhao Z, and Dai G
- Subjects
- Animals, Cecum parasitology, Cell Line, Cytokines metabolism, Cytokines genetics, Gene Expression Regulation, Inflammation genetics, Inflammation immunology, Inflammation parasitology, Signal Transduction, Transcriptome, Male, Female, Chickens, Coccidiosis veterinary, Coccidiosis immunology, Coccidiosis genetics, Coccidiosis parasitology, Eimeria tenella, Gene Expression Profiling, MicroRNAs genetics, Poultry Diseases parasitology, Poultry Diseases genetics, Poultry Diseases immunology
- Abstract
Coccidiosis is an important parasitic protozoan disease in poultry farming, causing huge economic losses in the global poultry industry every year. MicroRNAs (miRNAs) are a class of RNA macromolecules that play important roles in the immune response to pathogens. However, the expression profiles and functions of miRNAs during Eimeria tenella (E. tenella) infection in chickens remain mostly uncharacterized. In this study, high-throughput sequencing of cecal tissues of control (JC), resistant (JR), and susceptible (JS) chickens led to the identification of 35 differentially expressed miRNAs among the three groups. Functional enrichment analysis showed that the differentially expressed miRNAs were mainly associated with the TGF-beta, NF-kB, and Jak-STAT signaling pathways. Notably, gga-miR-2954 was found to be significantly upregulated after coccidial infection. Functional analysis showed that gga-miR-2954 inhibited the production of the inflammatory cytokines IL-6, IL-1β, TNF-α, and IL-8 in sporozoite-stimulated DF-1 cells. Mechanistically, we found that gga-miR-2954 targeted the RORC gene and that RORC promoted the inflammatory response in sporozoite-stimulated DF-1 cells. In conclusion, our study was the first to identify differentially expressed miRNAs in chicken cecal tissue during E. tenella infection and found that gga-miR-2954 regulates the host immune response to coccidial infection in chickens by targeting the RORC gene., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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