Search

In vitro studies of gas production (GP) using rumen fluid obtained from rumen-cannulated animals are common in feed evaluation for ruminants. The objective was to compare sheep faeces with rumen fluid from dairy cows as inocula in the Hohenheim gas test (HGT) using a large number of different-quality feeds. It then was evaluated whether GP obtained by using rumen inoculum (RI-GP) can be estimated from GP using faecal inoculum (FI-GP). Ninety feeds for ruminants including roughages (n = 27), total mixed rations (n = 6), commercial compound feeds for dairy cows (n = 18), cereal grains (n = 10), energy-rich by-products (n = 5), legume grains (n = 6), oilseed meals (n = 6), and other protein-rich feeds (n = 12) were incubated with either rumen inoculum (RI) or faecal inoculum (FI), and GP was recorded at 2, 4, 8, 12, 24, 48, and 72 h. Nonlinear equations were fitted to the GP data to determine GP kinetic parameters. Correlations between RI-GP and FI-GP were calculated for different time points. Linear regressions were calculated to estimate RI-GP from FI-GP using the entire data set or subsets of roughages and concentrates. GP kinetic parameters were lower for all feed categories when incubated with FI instead of RI. On average of all feed samples, the potential GP was 9 mL/200 mg dry matter (DM) lower and the rate of GP was 3.1%/h lower with FI than RI. The estimation of kinetic data for FI included a lag phase of 1.51 h on average, whereas no lag phase was estimated for RI. Estimated parameters indicated an overall lower fermentation activity of FI than RI. However, there was a very similar progression of GP curves of RI-GP and FI-GP. RI-GP after 24 h was significantly correlated with FI-GP in the time period of 24 to 60 h (r = 0.973 – 0.982, including all feed samples). Linear regression analysis showed that RI-GP after 24 h can be estimated from FI-GP after 24 h (Slope = 1.02, R2 = 0.97) and 48 h (Slope = 1.1, R2 = 0.97) including the complete data set. Splitting the data set into roughages and concentrate feeds did not lead to distinctly higher estimation accuracy. In conclusion, sheep faeces can be used as an alternative inoculum for studying a wide range of different-quality feedstuffs. The standard gas test may be modified without the need for rumen-cannulated animals. [ABSTRACT FROM AUTHOR]

BACKGROUND AND OBJECTIVES: Trichoderma species (Trichoderma sp.) is commonly found in nature, particularly in soil and roots, often thriving in plants rich in cellulose such as oil palm. Therefore, this study aimed to characterize Trichoderma sp. in each substrate as well as to assess cellulolytic and ligninolytic potential in vitro using a specific medium. Characterization and utilization of the plant were examined to efficiently manage empty fruit bunch (EFB) waste in Indonesian oil palm plantations. By exploring the biological, ecological, and biochemical attributes of Trichoderma sp., this study examined the mechanisms associated with efficacy in bioconversion processes and waste remediation. METHODS: A survey method was used to determine sampling locations, and purposive sampling was utilized in the field. Furthermore, several stages were undertaken including isolation, identification, as well as testing of morphology and potential in vitro. FINDINGS: The results showed that the growth characteristics of Trichoderma sp. in filling a petri dish spanned approximately five days, forming colonies with a dark green hue. Microscopically, the isolates consisted of conidiophores, phialides, and conidia. Meanwhile, in vitro potential was assessed through cellulolytic and lignocellulolytic assays, with each isolate demonstrating varying capabilities in breaking down cellulose and lignocellulose. The highest cellulase activity (3.55 units per gram) was observed in enzyme starter granules treated with air drying. The highest viability per gram was found in spore inoculum (17.0 x 107) with air drying treatment. The greatest reduction in weight of oil palm EFB waste (65.3 percent) was achieved through the application of Trichoderma sp. enzyme starter granules with air drying treatment. Furthermore, eight isolates demonstrated cellulolytic and ligninolytic potential. Isolates sourced from soil had rapid growth on starter and baglog mediums, indicating potential for subsequent field applications as a biodegradation agent for organic waste. CONCLUSION: Based on the macroscopic (shape and color of mycelium) and microscopic characteristics (mycelium form), a total of eight Trichoderma sp. isolates were obtained. During the potential test, it was observed each isolate had different cellulolytic and ligninolytic activities. This study provided valuable insights into the diversity and enzymatic potential of Trichoderma sp. fungi, underscoring the importance of selecting suitable strains and optimizing cultivation conditions for various biotechnological applications. By exploring cellulolytic and ligninolytic capabilities, the results contributed to the development of more efficient and sustainable strategies for organic waste management and bioconversion. In general, this study represented a significant milestone in the quest for sustainable waste management solutions in agriculture, offering a promising pathway towards mitigating environmental impact and promoting the long-term sustainability of oil palm cultivation. [ABSTRACT FROM AUTHOR]

Deciduous forests in northeastern North America are comprised of a mixture of ectomycorrhizal (EM) and arbuscular mycorrhizal (AM) trees. However, land use legacies and other factors have decreased EM and concomitantly increased AM tree dominance, potentially limiting forest ecosystem functioning. We investigated how scattered EM tree patches and soil inoculum from local EM tree‐dominated forests influence EM fungal colonisation, survival and growth of EM Tsuga canadensis, Pinus strobus and Quercus spp. tree seedlings in AM‐dominated secondary forests. Seedlings were planted next to dominant AM Acer and scattered EM Betula trees across three New York secondary forests. A subset of seedlings also received soil inoculum from local EM‐dominated forests. We monitored the survival and height growth of seedlings for 2 years and then measured seedling shoot biomass, assessed EM colonisation and identified EM fungi from seedling roots. All genera of seedlings planted near EM Betula had higher EM colonisation and fungal richness than those planted near AM Acer trees. Inoculation with EM forest soil increased EM colonisation and fungal richness of seedlings only near AM Acer, showing great effectiveness in areas lacking native EM fungi. Total EM fungal diversity on seedling roots was highest near EM Betula, which included numerous taxa usually associated with mature trees. In contrast, EM fungal communities near AM Acer were sparse and mostly dominated by relatively few taxa of spore‐bank fungi. Although seedling survival did not differ between treatments, soil inoculation and proximity to EM Betula increased second‐year height growth of Pinus and Tsuga while soil inoculation alone significantly enhanced shoot mass of Quercus seedlings. Synthesis. Agricultural land use legacies have resulted in expansive secondary forests dominated by AM trees. In these forests, establishment of EM tree seedlings outside existing EM tree patches may be hindered by a lack of EM fungi, but local soil inoculum from EM tree‐dominated forests can reintroduce native EM fungi into secondary forests lacking established EM trees. [ABSTRACT FROM AUTHOR]

Compost is a natural and sustainable way to improve soil fertility and enhance plant growth. Moringa leaves have high mineral, cytokinin, and vitamin content which are useful for growth so that they can be used as organic fertilizer. Azotobacter and Streptomyces are from soil and have many biological activities. This study aimed to detect the importance of bioagents formula with Moringa Compost (MC) to enhance plant growth in poor sterile soil and plants were irrigated with half strength of Hoagland nutrient solution. Moringa leaves were collected and cleaned, and organic compost was prepared and analyzed for microbial and chemical composition. The prepared MC was rich in nitrogen and minerals and had high content of bacteria and fungi. The two bioagents used were isolate MB5 and MB11 which were characterized and molecular identified as Azotobacter chroococcum MB5 and Streptomyces griseus MB11. The free-living A. chroococcum can fix atmospheric nitrogen while Streptomyces is a filamentous bacterium with a high ability to produce secondary metabolites. The addition of 20% MC to soil increased soil EC and microbial counts compared to MC-free soil. Moreover, inoculation of soil with either AZ or ST increased the microbial counts and soil EC and the clearest increase was in the case of inoculation of soil with MC+AZ+ST. It also found that MC extract alone with the bacterial filtrates increases seed germination of Phaseolus vulgaris L. (common bean), which is a herbaceous annual worldwide plant, grown for its edible dry seeds or green unripe pods. In this regard, inoculation of soil with inoculum of both A. chroococcum MB5, and S. griseus MB11, in the presence of MC has the most pronounced effect and enhances both the growth, fresh and dry weights, leaf number, plant height, and root length of P. vulgaris grown under greenhouse conditions for one month and chemical content of the plant protein carbohydrates, P, N, Ca++ and K+. In conclusion, the combined application A. chroococcum MB5 and S. griseus MB11, as a biofertilizers with Moringa compost is recommended to enhance P. vulgaris growth. The use of these biofertilizers can reduce the use of chemical fertilizers, which can have detrimental effects on soil and the environment. Therefore, further research on the inoculation and application of these microorganisms with MC is essential for sustainable agriculture. [ABSTRACT FROM AUTHOR]

The waste generated by the mezcal and seafood restaurant industries has significant negative environmental impacts on soils, water, and air. However, these waste materials offer the potential for methane production through anaerobic digestion. This research focuses on evaluating a methodological strategy that involves using an enriched inoculum, co-digesting waste materials, and applying specific pretreatment (chemical, thermal, and adjusting the volumetric ratio of residues) to produce biogas with a high methane content, as assessed through the Biochemical Methane Potential (BMP) test. The results demonstrate that the enriched inoculum used in the test exhibited a pH of 8.18, an Alkalinity of 31 g CaCO3/L, and an alpha (α) ratio of 0.75. The BMP test was conducted over 29 days. In the most successful test, which involved pretreatment at a temperature of 50 °C, a residue ratio of 1 VSW (Volume of Seafood Waste)/2 VMV (Volume of Mezcal Vinasses), and a pH of 6.5, we achieved a methane production of 1420.51 ± 0.038 N mL CH4, with a biogas containing 81.5% methane. This process also yielded 175.37 N mL CH4 per gram of Volatile Solids (VS) and a degradation kinetic constant of 0.14 1/d. Through the methodological strategy used with the combination of an enriched and stabilized inoculum, the co-digestion of the waste for nutrient complementation, and the application of pretreatments, it was achieved that the different stages that constitute the anaerobic process allowed for the establishment of optimal conditions for the generation of biogas with a high methane content. [ABSTRACT FROM AUTHOR]

Hydrogen (H2) is a highly efficient and clean energy source with the potential for renewable energy. The production of H2 from biological routes such as biophotolysis, photofermentation, dark fermentation, and bioelectrochemical production is characterized as a renewable alternative to current production, which is mainly based on energy-intensive electrochemical and thermochemical processes and responsible for the emission of high amounts of environmentally harmful compounds. Dark fermentation is the most efficient and cost-effective method for producing biohydrogen, making it a key research focus. This article offers a comprehensive overview of the dark fermentation process with the aim of enhancing hydrogen productivity and yields. Aspects related to the main substrates used, the inoculum sources and their pretreatment, and physical-chemical parameters of the process are covered. Furthermore, this manuscript addresses topics such as process integration, genetic and metabolic engineering of fermentative microorganisms, and the main types of bioreactors aimed at greater yields and productivity of biohydrogen to enable its production through dark fermentation on a larger scale. [ABSTRACT FROM AUTHOR]

BACKGROUND AND OBJECTIVES: Trichoderma species (Trichoderma sp.) is commonly found in nature, particularly in soil and roots, often thriving in plants rich in cellulose such as oil palm. Therefore, this study aimed to characterize Trichoderma sp. in each substrate as well as to assess cellulolytic and ligninolytic potential in vitro using a specific medium. Characterization and utilization of the plant were examined to efficiently manage empty fruit bunch (EFB) waste in Indonesian oil palm plantations. By exploring the biological, ecological, and biochemical attributes of Trichoderma sp., this study examined the mechanisms associated with efficacy in bioconversion processes and waste remediation.METHODS: A survey method was used to determine sampling locations, and purposive sampling was utilized in the field. Furthermore, several stages were undertaken including isolation, identification, as well as testing of morphology and potential in vitro.RESULTS: The results showed that the growth characteristics of Trichoderma sp. in filling a petri dish spanned approximately five days, forming colonies with a dark green hue. Microscopically, the isolates consisted of conidiophores, phialides, and conidia. Meanwhile, in vitro potential was assessed through cellulolytic and lignocellulolytic assays, with each isolate demonstrating varying capabilities in breaking down cellulose and lignocellulose. The highest cellulase activity (3.55 units per gram) was observed in enzyme starter granules treated with air drying. The highest viability per gram was found in spore inoculum (17.0 x 107) with air drying treatment. The greatest reduction in weight of oil palm EFB waste (65.3 percent) was achieved through the application of Trichoderma sp. enzyme starter granules with air drying treatment. Furthermore, eight isolates demonstrated cellulolytic and ligninolytic potential. Isolates sourced from soil had rapid growth on starter and baglog mediums, indicating potential for subsequent field applications as a biodegradation agent for organic waste.CONCLUSION: Based on the macroscopic (shape and color of mycelium) and microscopic characteristics (mycelium form), a total of eight Trichoderma sp. isolates were obtained. During the potential test, it was observed each isolate had different cellulolytic and ligninolytic activities. This study provided valuable insights into the diversity and enzymatic potential of Trichoderma sp. fungi, underscoring the importance of selecting suitable strains and optimizing cultivation conditions for various biotechnological applications. By exploring cellulolytic and ligninolytic capabilities, the results contributed to the development of more efficient and sustainable strategies for organic waste management and bioconversion. In general, this study represented a significant milestone in the quest for sustainable waste management solutions in agriculture, offering a promising pathway towards mitigating environmental impact and promoting the long-term sustainability of oil palm cultivation.

Hydrogen (H2) is a highly efficient and clean energy source with the potential for renewable energy. The production of H2 from biological routes such as biophotolysis, photofermentation, dark fermentation, and bioelectrochemical production is characterized as a renewable alternative to current production, which is mainly based on energy-intensive electrochemical and thermochemical processes and responsible for the emission of high amounts of environmentally harmful compounds. Dark fermentation is the most efficient and cost-effective method for producing biohydrogen, making it a key research focus. This article offers a comprehensive overview of the dark fermentation process with the aim of enhancing hydrogen productivity and yields. Aspects related to the main substrates used, the inoculum sources and their pretreatment, and physical-chemical parameters of the process are covered. Furthermore, this manuscript addresses topics such as process integration, genetic and metabolic engineering of fermentative microorganisms, and the main types of bioreactors aimed at greater yields and productivity of biohydrogen to enable its production through dark fermentation on a larger scale.

Compost is a natural and sustainable way to improve soil fertility and enhance plant growth. Moringa leaves have high mineral, cytokinin, and vitamin content which are useful for growth so that they can be used as organic fertilizer. Azotobacter and Streptomyces are from soil and have many biological activities. This study aimed to detect the importance of bioagents formula with Moringa Compost (MC) to enhance plant growth in poor sterile soil and plants were irrigated with half strength of Hoagland nutrient solution. Moringa leaves were collected and cleaned, and organic compost was prepared and analyzed for microbial and chemical composition. The prepared MC was rich in nitrogen and minerals and had high content of bacteria and fungi. The two bioagents used were isolate MB5 and MB11 which were characterized and molecular identified as Azotobacter chroococcum MB5 and Streptomyces griseus MB11. The free-living A. chroococcum can fix atmospheric nitrogen while Streptomyces is a filamentous bacterium with a high ability to produce secondary metabolites. The addition of 20% MC to soil increased soil EC and microbial counts compared to MC-free soil. Moreover, inoculation of soil with either AZ or ST increased the microbial counts and soil EC and the clearest increase was in the case of inoculation of soil with MC+AZ+ST. It also found that MC extract alone with the bacterial filtrates increases seed germination of Phaseolus vulgaris L. (common bean), which is a herbaceous annual worldwide plant, grown for its edible dry seeds or green unripe pods. In this regard, inoculation of soil with inoculum of both A. chroococcum MB5, and S. griseus MB11, in the presence of MC has the most pronounced effect and enhances both the growth, fresh and dry weights, leaf number, plant height, and root length of P. vulgaris grown under greenhouse conditions for one month and chemical content of the plant protein carbohydrates, P, N, Ca++ and K+. In conclusion, the combined application A. chroococcum MB5 and S. griseus MB11, as a biofertilizers with Moringa compost is recommended to enhance P. vulgaris growth. The use of these biofertilizers can reduce the use of chemical fertilizers, which can have detrimental effects on soil and the environment. Therefore, further research on the inoculation and application of these microorganisms with MC is essential for sustainable agriculture.

The present study compared the supplementation of Litopenaeus vannamei tanks with inoculums generated by different nitrogen sources (NH4Cl or feed). The interactions of inoculums and C/N adjustment strategies on water quality and growth performance were also investigated. After preparation of inoculums, shrimp juveniles were reared for 39 days at 142 shrimps/m3 in four treatments: T1, NH4Cl-based inoculum + heterotrophic C/N adjustment; T2, NH4Cl-based inoculum + mixotrophic C/N adjustment; T3, feed-based inoculum + heterotrophic C/N adjustment; and T4, feed-based inoculum + mixotrophic C/N adjustment. At the end, two-way ANOVA analysis showed significant interactions of time × inoculum or time × C/N adjustment strategy on dissolved oxygen, pH, and settleable solids. Alkalinity was significantly affected by the time × C/N adjustment strategy interaction, while inorganic nitrogen species (TAN, NO2-N, and NO3-N) were altered depending on the time × inoculum effect. Inoculum and C/N adjustment strategy significantly altered alkalinity and TAN levels. TAN levels in T1 and T2 treatments accumulated for 2 weeks, and organic carbon addition was not effective in mitigating high TAN levels. The mixotrophic strategy significantly reduced solids levels, while enhancing DO levels. No significant interactive effects of inoculum × C/N adjustment strategy were detected on the water quality indicators. The final body weight (FBW), feed conversion ratio (FCR), and weekly growth rate (WGR) of shrimp were significantly improved in tanks supplied with feed-based inoculum. Also, the inoculum × C/N adjustment strategy led to significantly higher FBW, weight gain, specific growth rate, and WGR in tanks supplied with feed-based inoculum and heterotrophic C/N adjustment. In conclusion, feed-based inoculum prevented the accumulation/peak of nitrogen species, reduced the nitrification establishment time, and enhanced growth performance. In addition, inoculum effectiveness was affected by the C/N adjustment strategy, highlighting the advantageous performance of mixotrophic approach. This information can develop efficient management practices for biofloc systems. [ABSTRACT FROM AUTHOR]

Abstract Bacterial canker disease caused by Clavibacter michiganensis is a substantial threat to the cultivation of tomatoes, leading to considerable economic losses and global food insecurity. Infection is characterized by white raised lesions on leaves, stem, and fruits with yellow to tan patches between veins, and marginal necrosis. Several agrochemical substances have been reported in previous studies to manage this disease but these were not ecofriendly. Thus present study was designed to control the bacterial canker disease in tomato using green fabricated silver nanoparticles (AgNps). Nanosilver particles (AgNPs) were synthesized utilizing Moringa oleifera leaf extract as a reducing and stabilizing agent. Synthesized AgNPs were characterized using UV–visible spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), energy-dispersive X-ray (EDX), and Fourier transform infrared spectrometry (FTIR). FTIR showed presence of bioactive compounds in green fabricated AgNPs and UV-visible spectroscopy confirmed the surface plasmon resonance (SPR) band in the range of 350 nm to 355 nm. SEM showed the rectangular segments fused together, and XRD confirmed the crystalline nature of the synthesized AgNPs. The presence of metallic silver ions was confirmed by an EDX detector. Different concentrations (10, 20, 30, and 40 ppm) of the green fabricated AgNPs were exogenously applied on tomato before applying an inoculum of Clavibacter michigensis to record the bacterial canker disease incidence at different day intervals. The optimal concentration of AgNPs was found to be 30 µg/mg that exhibited the most favorable impact on morphological (shoot length, root length, plant fresh and dry weights, root fresh and dry weights) and physiological parameters (chlorophyll contents, membrane stability index, and relative water content) as well as biochemical parameters (proline, total soluble sugar and catalase activity). These findings indicated a noteworthy reduction in biotic stress through the increase of both enzymatic and non-enzymatic activities by the green fabricated AgNPs. This study marks a first biocompatible approach in assessing the potential of green fabricated AgNPs in enhancing the well-being of tomato plants that affected with bacterial canker and establishing an effective management strategy against Clavibacter michiganensis. This is the first study suggests that low concentration of green fabricated nanosilvers (AgNPs) from leaf extract of Moringa oleifera against Clavibacter michiganensis is a promisingly efficient and eco-friendly alternative approach for management of bacterial canker disease in tomato crop.

The efficiency of anaerobic night soil (human excreta) biodegradation depends on the characteristics of the inoculum used. This anaerobic microbial inoculum (AMI) contains a microbial consortium, including rate-limiting methanogens whose growth depends on various physicochemical and biological parameters necessitating the inoculum characterisation essential for optimum night soil (NS) biodegradation. The inoculum contained 24.567 g/L (±0.07) total solid, 5.022 (±0.57) g/L total dissolved solid, 18.148 (±0.0.058) g/L volatile solid (VS), and 7.0±0.2 pH. The Gas chromatography analysis confirmed the presence of a small amount of volatile fatty acid (VFA), (≤ 4 mM except propionate) showing rapid conversion of VFA to methane, and 55% methane. Further, the presence of methanosaetaceae group of methanogens was detected through Taqman probe-based real-time PCR. The inoculum performance was evaluated for NS biodegradation in semicontinuous mode of feeding in four 2 L anaerobic biodigesters (2D HRT, 4D HRT, 5D HRT, and 10D HRT) with varying hydraulic retention time (HRT) (2, 5, 8, and 10 days, respectively) at mesophilic temperature (35°C). After 5 HRT, VS reduction for 2D HRT and 5D HRT was 45-50% and >45-60%, respectively. The methane content was 45-50% and 50-65% methane, respectively. The % VS reduction and methane content showed the overfeeding of NS in biodigesters 2D HRT and 5D HRT while 8D HRT showed towards optimum and 10D HRT optimum with improved VS reduction and methane content.

Biogas, derived from organic waste treatment, harnesses methane for clean energy, reducing waste and bolstering economic sustainability. In this study we evaluate the physiochemical analysis of the waste samples, generated biogas from a well design biodigester, purified the biogas generated and characterize both the untreated and treated biogas. The physicochemical analysis was carried out using a standard method, the gases were characterized using of GC-MS. The result of the physicochemical analysis of the domestic wastes includes; Moisture content (55.2-59.2 %), Total solid (40.8-44.7 %), Volatile solid (76.5-80.1%) and Carbon content (41.7-44.7%). The untreated biogas generated revealed CH4 (61.2 %), CO2 (36.7 %), CO (1.3 %), NH3 (0.5 %), and H2S (0.3 %) with a calorific value of 23.41 MJ/m3, while the treated biogas revealed CH4 (70 %), CO2 (28 %), CO (1.3 %), NH3 (0.5 %), H2S (0.2 %) with a calorific value of 26.78 MJ/m3. In addition, the production and use of biogas contribute to sustainability by providing access to contemporary, clean energy that is dependable, as well as by reducing emissions and reducing climate change's effects. [ABSTRACT FROM AUTHOR]

Restoring native plant species on degraded landscapes is challenging. Symbiotic partners in the plant rhizosphere can aid in nutrient acquisition, pathogen protection, stress tolerance, and many other processes. However, these microbes are often absent in altered landscapes and need to be re-integrated to improve restoration efforts. We evaluated, within a laboratory setting, the ability of commercial and indigenous rhizobia strains to form nodules on lupine species used for rangeland seedings in the Great Basin region of the Western United States and ascertained if these strains could be applied through a seed coating. We also evaluated if a compost amendment applied via seed coating could further enhance the performance of the rhizobia strains. Our analysis showed that successful nodulation could occur using commercial and wildland-collected indigenous strains through either a liquid culture applied to seedlings or as a dry seed coating. However, the number of root nodules and the presence of a pink color (indicating nitrogen fixation) were typically higher in the commercial product than in the indigenous strains. Compost did not improve nodulation or the performance of the nodules; however, this treatment alone improved shoot growth. Overall, these results suggest that commercial rhizobium may be more effective in improving plant growth, and future research with native rhizobia may want to consider identifying strains compatible with seed-coating delivery. Longer-term studies are now merited for assessing how the rhizobia strains evaluated in this study influence plant growth, particularly in a field setting. [ABSTRACT FROM AUTHOR]

Biological nitrogen fixation (BNF) can provide the necessary nitrogen for bean crops; however, for this to occur, important limitations involving the inoculant application technology need to be overcome.The use of co-inoculation is a management technique used to obtain benefits and increase the potential of N2 fixation from the association between bacteria from the rhizobia group, such as R. tropici, and bacteria that promote plant growth, such as A. brasilense, in association with the addition of nutrients that allow greater efficiency of bacteria fixing atmospheric N2. This study aimed to evaluate the bean response to the reinoculation of R. tropici in co-inoculation with A. brasilense in a mixture with the micronutrients Co/Mo, in the winter season of 2021, in Anápolis-GO, Brazil. A randomized block design was used, with four replications, and the following treatments (TRs) were studied: TR1—reinoculation with R. tropici; TR2—reinoculation with co-inoculation of R. tropici + A. brasilense; TR3—reinoculation of R. tropici + Mo/Co micronutrients; TR4—reinoculation with co-inoculation R. tropici + A. brasilense + Mo/Co micronutrients; TR5—inoculation via seed, without reinoculation; TR6—mineral N fertilization in the sowing furrow and topdressing; TR7—control, without any N source. At stage R6, nodulation characteristics (number and dry mass of nodules) and the morphophysiological parameters of the plants (main root length, root dry mass, plant height, shoot dry mass, leaf area, and leaf N content in the shoot) were evaluated. At harvest, the final plant stand and components (number of pods per plant, number of grains per pod, and average weight of one hundred grains) were determined, in addition to grain yield. It was concluded that inoculation followed by reinoculation in topdressing with R. tropici in co-inoculation with A. brasilense plus Mo/Co, compared to mineral nitrogen fertilization, improves the efficiency of the nodulation process and the morphophysiological characteristics of the common bean crop. Seed inoculation and topdressing application with R. tropici, associated with co-inoculation with A. brasilense + Mo and Co, have the potential to completely replace mineral nitrogen fertilization in common bean crops. [ABSTRACT FROM AUTHOR]

Aims Incorporating biofertilizers, such as arbuscular mycorrhizal fungal (AM) fungal inoculants, into vineyard management practices may enhance vine growth and reduce environmental impact. Here, we evaluate the effects of commercially available and local AM fungal inoculants on the growth, root colonization, and nutrient uptake of wine grapes (Vitis vinifera) when planted in a field soil substrate. Methods and results In a greenhouse experiment, young wine grapes were planted in a field soil substrate and inoculated with one of three commercially available mycorrhizal inoculant products, or one of two locally collected whole soil inoculants. After 4 months of growth, inoculated vines showed no differences in plant biomass, colonization of roots by AM fungi, or foliar macronutrient concentrations compared to uninoculated field soil substrate. However, vines grown with local inoculants had greater shoot biomass than vines grown with mycorrhizal inoculant products. Conclusions Although effects from inoculations with AM fungi varied by inoculant type and source, inoculations may not improve young vine performance in field soils with a resident microbial community. [ABSTRACT FROM AUTHOR]

Carbapenem-resistant Pseudomonas aeruginosa infections represent a critical public health concern, highlighting the need for the development of effective antibiotics. Cefiderocol demonstrated potent in vitro activity against Pseudomonas aeruginosa, particularly in strains that are resistant to other drugs. However, concerns regarding the emergence of drug-resistant strains persist. This study, conducted with 109 carbapenem-resistant Pseudomonas aeruginosa strains from the Spanish Hospital (Dr. Balmis, Alicante). The study evaluated susceptibility to cefiderocol in comparison to alternative antibiotics and including their susceptibility to bacterial inoculum, while assessing various testing methods. Our findings revealed high susceptibility to cefiderocol against carbapenem-resistant strains, with only 2 of 109 strains exhibiting resistance. Comparative analysis demonstrated superiority of cefiderocol towards alternative antibiotics. Both the E-test and disk-diffusion methods showed 100% concordance with the microdilution method in classifying strains as susceptible or resistant. However, 4.6% (5/109) of disc zone diameters fell within the technical uncertainty zone, so the E-test technique was found to be more useful in routine clinical practice. Additionally, escalating bacterial inoculum correlated with decreases in vitro activity, so this parameter should be adjusted very carefully in in vivo studies. This study underscores cefiderocol's potential as a therapeutic option for carbapenem-resistant Pseudomonas aeruginosa infections. However, the emergence of drug-resistant strains emphasizes the critical need for a wise use of antibiotics and a continuous monitoring of resistance to antibiotics. Based on our in vitro data, further investigation concerning the impact of bacterial inoculum on drug efficacy is warranted in order to detect resistance mechanisms and optimize treatment strategies, thereby mitigating the risk of resistance. [ABSTRACT FROM AUTHOR]

Bacterial canker disease caused by Clavibacter michiganensis is a substantial threat to the cultivation of tomatoes, leading to considerable economic losses and global food insecurity. Infection is characterized by white raised lesions on leaves, stem, and fruits with yellow to tan patches between veins, and marginal necrosis. Several agrochemical substances have been reported in previous studies to manage this disease but these were not ecofriendly. Thus present study was designed to control the bacterial canker disease in tomato using green fabricated silver nanoparticles (AgNps). Nanosilver particles (AgNPs) were synthesized utilizing Moringa oleifera leaf extract as a reducing and stabilizing agent. Synthesized AgNPs were characterized using UV–visible spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), energy-dispersive X-ray (EDX), and Fourier transform infrared spectrometry (FTIR). FTIR showed presence of bioactive compounds in green fabricated AgNPs and UV-visible spectroscopy confirmed the surface plasmon resonance (SPR) band in the range of 350 nm to 355 nm. SEM showed the rectangular segments fused together, and XRD confirmed the crystalline nature of the synthesized AgNPs. The presence of metallic silver ions was confirmed by an EDX detector. Different concentrations (10, 20, 30, and 40 ppm) of the green fabricated AgNPs were exogenously applied on tomato before applying an inoculum of Clavibacter michigensis to record the bacterial canker disease incidence at different day intervals. The optimal concentration of AgNPs was found to be 30 µg/mg that exhibited the most favorable impact on morphological (shoot length, root length, plant fresh and dry weights, root fresh and dry weights) and physiological parameters (chlorophyll contents, membrane stability index, and relative water content) as well as biochemical parameters (proline, total soluble sugar and catalase activity). These findings indicated a noteworthy reduction in biotic stress through the increase of both enzymatic and non-enzymatic activities by the green fabricated AgNPs. This study marks a first biocompatible approach in assessing the potential of green fabricated AgNPs in enhancing the well-being of tomato plants that affected with bacterial canker and establishing an effective management strategy against Clavibacter michiganensis. This is the first study suggests that low concentration of green fabricated nanosilvers (AgNPs) from leaf extract of Moringa oleifera against Clavibacter michiganensis is a promisingly efficient and eco-friendly alternative approach for management of bacterial canker disease in tomato crop. [ABSTRACT FROM AUTHOR]

This study focused on determining the effect of the inoculum to substrate ratio (ISR) on biogas production efficiency from the anaerobic co-digestion of two substrates: synthetic food waste and common reeds (Phragmites australis) that were ground and pre-treated using sodium hydroxide at a concentration of 2% to increase access to their cellulose. It also studied the role of different mixing ratios of the two substrates in improving the stability of the digestion process and increasing biogas production. A series of batch tests were carried out under mesophilic conditions using three ratios of ISR: 1:4, 1:2, and 1:1, and five substrate mixing ratios (synthetic food waste: pre-treated P. australis): 25:75, 50:50, 75:25, 100:0, and 0:100. The results showed low biogas production at the ISR 1:4 (21.58±0.00–44.46±0.01 mL/g volatile solid (VS) added), and the reactors suffered from acidification at the different substrates mixing ratios, while the biogas production increased at an ISR of 1:2, where the reactors with the substrate mixing ratio of 25:75 presented the highest biogas production (82.17±0.62 mL/g VS added), and the digestion process was stable. However, the reactors with substrate mixing ratios of 50:50, 75:25, and 100:0 suffered from acidification effects at this ISR. In contrast, at ISR of 1:1, the reactors did not expose to acidification inhibition at all the substrates mixing ratios, and the highest biogas production was found at synthetic food waste: pre-treated P. australis mixing ratios of 75:25 and 100:0 (76.15±1.85 and 82.47±1.85 mL/g VS added, respectively). [ABSTRACT FROM AUTHOR]

Over the past three decades, growing global interest has been in replacing fossil fuels with clean energy from renewable resources. As a result, biogas generation from biodegradable organic waste has become increasingly popular, and water hyacinth is a menace wherever it exists. This study aimed to assess the effectiveness of water hyacinth (Eichhornia crassipes) from River Wouri as a biogas substrate. Equal volumes (700 mL) of water hyacinth and poultry-based inoculum were prepared, each with a duplicate. The 8 substrates were placed in 1-liter plastic and incubated in a water bath at 37± 2 °C for 106 days. The substrates were subsampled before and after incubation to analyse the dry matter, moisture, volatile solids, ash contents, and pH. The volume of biogas produced from each digester was collected in plastic bags and measured every three days; hence, the methane produced was calculated. Results indicated an increase in the moisture contents of the substrates, while pH levels remained unchanged after incubation. The rate of destruction of volatile solids increased with the level of added inoculum. Biogas production started almost immediately for all the samples and largely followed a dome shape over the incubation period. Cumulative methane produced were 10.23, 11.84, 5.88, and 0.71 LCH4/kgVS, respectively, for 100% water hyacinth, 90% water hyacinth, 80% water hyacinth and 0% water hyacinth. This study demonstrates the effectiveness of the weed in biogas production, which could be an important method to manage River Wouri while generating renewable energy. [ABSTRACT FROM AUTHOR]

The efficiency of anaerobic night soil (human excreta) biodegradation depends on the characteristics of the inoculum used. This anaerobic microbial inoculum (AMI) contains a microbial consortium, including rate-limiting methanogens whose growth depends on various physicochemical and biological parameters necessitating the inoculum characterisation essential for optimum night soil (NS) biodegradation. The inoculum contained 24.567 g/L (±0.07) total solid, 5.022 (±0.57) g/L total dissolved solid, 18.148 (±0.0.058) g/L volatile solid (VS), and 7.0±0.2 pH. The Gas chromatography analysis confirmed the presence of a small amount of volatile fatty acid (VFA), (≤ 4 mM except propionate) showing rapid conversion of VFA to methane, and 55% methane. Further, the presence of methanosaetaceae group of methanogens was detected through Taqman probe-based real-time PCR. The inoculum performance was evaluated for NS biodegradation in semicontinuous mode of feeding in four 2 L anaerobic biodigesters (2D HRT, 4D HRT, 5D HRT, and 10D HRT) with varying hydraulic retention time (HRT) (2, 5, 8, and 10 days, respectively) at mesophilic temperature (35°C). After 5 HRT, VS reduction for 2D HRT and 5D HRT was <40% while for 8D HRT and 10D HRT was >45-50% and >45-60%, respectively. The methane content was <20% in biogas for 2 and 5 days HRT while for 8 and 10 days >45-50% and 50-65% methane, respectively. The % VS reduction and methane content showed the overfeeding of NS in biodigesters 2D HRT and 5D HRT while 8D HRT showed towards optimum and 10D HRT optimum with improved VS reduction and methane content. [ABSTRACT FROM AUTHOR]

In the face of persistent soil degradation in Benin caused by poor agricultural practices, including excessive use of chemical fertilizers, it is urgent to find solutions that take into account the microorganisms of interest. This study aimed to assess the effect of combining three strains of indigenous arbuscular mycorrhizal fungi (AMF) on maize production in northern Benin. The study involved 34 growers in Ouénou, Bagou and Kokey. The experimental setup consisted of three elementary plots with three treatments. Growth parameters were measured every 15 days, from the 15th to the 60th day after sowing, on ten plants per plot. Plant nutritional status, grain yield and mycorrhization were measured. The results showed that biostimulant + 50% NPK_Urea (N = nitrogen, P = phosphorus and K = potassium) had similar positive effects on growth parameters to those induced by the application of 100% NPK_Urea. Gains of 30.25% to 36.35% were recorded in plant height at Kokey. On the other hand, biostimulant+ 50% NPK_Urea induced a better phosphorus uptake of 21.08% to 27.77%. In addition, the grain yield of mycorrhizal plants was 8.37% higher than that of plants receiving 100% NPK_Urea at Ouénou. These results show that this technology could be integrated into the agricultural system to promote sustainable maize growing in Benin. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate the effect of different management strategies on the toxic nitrogen compounds and alkalinity patterns in nurseries and pre-grow-out of Penaeus vannamei culture with low salinity water (2.5 g L−1) and synbiotic system. A study case is presented using three individual experiments (E): two nurseries (EI and EII: 2000 shrimp m−3 or 600 shrimp m−2) and one pre-grow-out (EIII: 300 shrimp m−3 or 90 shrimp m−2). Key water quality management strategies included the adoption of artificial substrate and water reuse with a microbial-based synbiotic system. In EI experiment, mean TAN was less than 0.50 mg L−1 throughout the culture, NO2−-N reached a maximum concentration of 0.80 mg L−1, and NO3−-N showed a decrease over the time. In EII, mean TAN was 0.64 mg L−1 with a peak on day 10 of the experimental course, with the same pattern occurring for NO2−-N. On the other hand, NO3−-N constantly increased from 0.10 mg L−1 at the beginning to 2.32 mg L−1 at the end of the trial. In EIII, mean TAN was less than 0.50 mg L−1 reaching a maximum concentration of 0.93 mg L−1; NO2−-N reached a maximum mean concentration of 0.70 mg L−1 at the end of the experiment. NO3−-N had a mean of 1.50 mg L−1 at the beginning of the experiment and a reduction to 0.53 mg L−1 at the end of the trial. Alkalinity showed mean concentrations of 114.90 mg L−1, 88.04 mg L−1, and 88.27 mg L−1 for EI, EII, and EIII, respectively. The results demonstrated that the proposed management protocols adopted in EI, EII, and EIII were efficient for toxic nitrogen control in culture conditions using low salinity water and synbiotic system. [ABSTRACT FROM AUTHOR]

Small slaughterhouses generate biowaste, which for economic reasons, is generally destined for composting. Inoculating appropriate microorganisms can improve biodegradation efficiency and mitigate odor generation during the composting process and can give rise to composts with neutral or pleasant odors. Therefore, the aim of this study was to compare the odor intensity reduction of compost generated with and without a formulated inoculum (Saccharomyces cerevisiae, Bacillus subtilis, and Rhodopseudomonas palustris). A set of experimental data was collected and analyzed according to the German "Verein Deutscher Ingenieure" odor protocol. The results showed that adding microorganisms was effective in reducing unpleasant odors in all three composts generated from swine, cattle, and poultry slaughterhouse by-products during both summer and winter seasons. Additionally, soil odor was predominant in composts that were inoculated in the two tested seasons (i.e., summer and winter). On the other hand, composts without inoculation had odors similar to peat for swine compost, ammonia for cattle compost, and manure for poultry compost, regardless of the season tested. Overall, composting process with appropriate inoculum can help in the correct disposal of slaughterhouse wastes by transforming organic matter into composts, which can have economic and environmental value as a soil conditioner and/or fertilizer. [ABSTRACT FROM AUTHOR]

Acidogenic fermentation is an emerging biotechnology that allows for the utilization of food waste as a feedstock to produce high-value products such as short-chain fatty acids (SCFAs), effectively offering a tangible solution for food waste management as well as resource recovery. The objectives of the current study were to identify the ideal inoculum, waste-activated sludge (WAS) or anaerobic digester sludge (AD), for the acidogenic fermentation of food waste at room temperature, as well as to evaluate the impact of heat pretreatment of these inoculums on fermentation performance. The maximum hydrolysis yield of 399 g sCOD/kg VS added was obtained when untreated AD was used as the inoculum, whereas the pretreated AD inoculum provided the highest SCFA yield and conversion efficiency of 238 g sCODSCFA/kg VS added and 71%, respectively. Heat pretreatment had a detrimental impact on the WAS inoculum, leading to lower hydrolysis and SCFA yields, but exerted a positive influence on the AD inoculum. The microbial community showed that heat pretreatment negatively impacted the abundance of non-spore-forming hydrolytic and acidogenic microorganisms. Overall, this study demonstrates the critical role of inoculum type and heat pretreatment in optimizing the acidogenic fermentation process, laying the groundwork for future refinements in SCFA production from food waste through inoculum design. [ABSTRACT FROM AUTHOR]

Pineapple (Ananas comosus) is commonly infected by Fusarium oxysporum, causal agent of the fusarium wilt disease. Conventionally, growers use synthetic fungicides to control the disease, which lead to environmental pollution, hazardous effects on non-target organisms and risks on human health. The aim of this work was to assess the effectiveness of Bacillus subtilis ANT01 and Rhizobium sp. 11B to control fusarium wilt on pineapple plants. Four treatments derived from a complete factorial design were tested under field conditions. Treatments composed of B. subtilis ANT01 and the combination B. subtilis ANT01-Rhizobium sp. 11B decreased disease severity by 94.4% and 86.1%, respectively. On the other hand, the treatment prepared with Rhizobium sp. 11B alone showed a reduction of 75.0%. Size of leaves and nutritional condition (SPAD units) of the biocontrol agents-treated plants showed no statistical differences. Moreover, B. subtilis ANT01 decreased by 46% the initial soil population of F. oxysporum, while Rhizobium sp. 11B, B. subtilis ANT01 plus Rhizobium sp. 11B and control, showed a population reduction of 12.5%, 24.2% and 23.0%, respectively. These results make evident the potential of B. subtilis ANT01 as biocontrol agent of the pathogen under field conditions. [ABSTRACT FROM AUTHOR]

Biogas, derived from organic waste treatment, harnesses methane for clean energy, reducing waste and bolstering economic sustainability. In this study we evaluate the physiochemical analysis of the waste samples, generated biogas from a well design biodigester, purified the biogas generated and characterize both the untreated and treated biogas. The physicochemical analysis was carried out using a standard method, the gases were characterized using of GC-MS. The result of the physicochemical analysis of the domestic wastes includes; Moisture content (55.2- 59.2 %), Total solid (40.8- 44.7 %), Volatile solid (76.5- 80.1%) and Carbon content (41.7- 44.7%). The untreated biogas generated revealed CH4 (61.2 %), CO2 (36.7 %), CO (1.3 %), NH3 (0.5 %), and H2S (0.3 %) with a calorific value of 23.41 MJ/ m3, while the treated biogas revealed CH4 (70 %), CO2 (28 %), CO (1.3 %), NH3 (0.5 %), H2S (0.2 %) with a calorific value of 26.78 MJ/ m3. In addition, the production and use of biogas contribute to sustainability by providing access to contemporary, clean energy that is dependable, as well as by reducing emissions and reducing climate change's effects.

Aerobic granular sludge (AGS) biotechnology has recently obtained considerable interest as a viable alternative to the activated sludge process (ASP) technique. This is because AGS can improve the performance of wastewater treatment and its significant capability for attaining sustainable growth. One of the main challenging issues for the aerobic granular sludge is the long startup time in wastewater treatment. This review presents a comprehensive analysis of AGS biotechnology, aiming specifically at its global adoption, startup duration, and granule stability. Aerobic granular sludge (AGS) is becoming more widespread globally. To ensure the successful implementation of AGS biotechnology, it is crucial to thoroughly identify the development of dense and stable granules, which is vital for the proper operation of wastewater treatment plants (WWTPs). Additionally, it offers a comprehensive summary of the latest advancements in the inoculum and polymer additives and their respective contributions to accelerate various processes through distinct mechanisms. In addition, this paper reviews the prevailing research patterns in the prompt initiation of rapid startup of AGS technology and outlines specific issues for future investigations.

Biohydrogen generation from various waste materials is quite promising in renewable energy exploration. Biohydrogen is a cost-effective biofuel that produces both water vapor and energy when burned. However, biohydrogen production is more appreciable in utilizing various waste materials, thereby compromising both socioeconomic and technical strategies of energy exploration. The substrate, inoculum employed and their concentrations, culture kinds, and pretreatment procedure have all been found to be important in biohydrogen production. Physiological variables such as pH, temperature, redox potential, and partial pressure also significantly impact biohydrogen generation. The utilization of several growth factors, mainly the substrate, nitrogen, and phosphorus, also confronts extensive applications during biohydrogen production. This present study explores the enhancing activity engaged by the parameters and focuses on the inhibitory effects of the operating conditions. • Less expensive bio-hydrogen production can be attained using facultative anaerobes. • Higher hydrogen production can be attained from the base-treated bacterial inoculum. • Temperature and pH are the prime factors confiding in more outstanding hydrogen production. • Protons liberated during organic acids breakdown would inhibit bacterial growth. • Ligninolytic enzyme-producing fungi are very promising in lignocellulose pretreatment. [ABSTRACT FROM AUTHOR]

Due to the slow growth rate of anammox bacteria, enriched sludge is required for the rapid start-up of anammox-based reactors. However, it is still unclear if long-term stored anammox sludge (SAS) is an effective source of inoculum to accelerate reactor start-up. This study explored the reactivation of long-term SAS and developed an efficient protocol to reduce the start-up period of an anammox reactor. Although stored for 13 months, a low level of the specific anammox activity of 28 mg N/g VSS/d was still detected. Experimental Phase 1 involved the direct application of SAS to an upflow sludge bed reactor (USB) operated for 90 d under varying conditions of hydraulic retention time and nitrogen concentrations. In Phase 2, batch runs were executed prior to the continuous operation of the USB reactor. The biomass reactivation in the continuous flow reactor was unsuccessful. However, the SAS was effectively reactivated through a combination of batch runs and continuous flow feed. Within 75 days, the anammox process achieved a stable rate of nitrogen removal of 1.3 g N/L/day and a high nitrogen removal efficiency of 84.1 ± 0.2%. Anammox bacteria (Ca. Brocadia) abundance was 37.8% after reactivation. These overall results indicate that SAS is a feasible seed sludge for faster start-up of high-rate mainstream anammox reactors. [ABSTRACT FROM AUTHOR]

The standardization of cultivation processes that allow high levels of conidia growth and formation is required to formulate Trichoderma products to combat fungal diseases in agronomically important crops. This study evaluated the effects of inoculation using different inoculum concentrations (1.0x105, 1.0x106, and 1.0x107 conidia mL-1) and inoculum volumes (10, 30, and 50 mL). Later, it evaluated the effect of adding microelements (CaCO3, KH2PO4, MgSO4*7H2O, and (NH4)2SO4) on the conidiogenesis of two strains of Trichoderma asperellum (GRB-HA01 and GRB-HA02) in solidstate and liquid fermentation processes. After 12 days of fermentation, the highest conidiogenesis values for Trichoderma asperellum GRB-HA01 (6.9x109±5.7x10² conidia g-1) and Trichoderma asperellum GRB-HA02 (1.3x109±1.4x10² conidia g-1) were achieved using an inoculum volume of 10 mL at a concentration of 1.0x07 conidia mL-1. Adding CaCO3 (1 g g-1), resulted in the highest conidia concentrations for Trichoderma asperellum GRB-HA01 (3.0x1011±2.5x10² conidia g-1) and Trichoderma asperellum GRB-HA02 (8.6x1010±1.1x10¹ conidia g-1), reducing fermentation times to 9 days. The conidiogenesis obtained with liquid fermentation was lower and affected Trichoderma asperellum GRB-HA01 (3.1x107±1.1x10² conidia g-1) and Trichoderma asperellum GRB-HA02 (3.1x109±2.8x10² conidia g-1). This study showed that inoculation and adding microelements were important factors in the conidiogenesis processes of Trichoderma asperellum GRB-HA01 and GRB-HA02. Additionally, it was evidenced that solid-state fermentations are more efficient than liquid fermentation processes. [ABSTRACT FROM AUTHOR]

Tomato brown rugose fruit virus (ToBRFV) is an economically important seed and mechanically transmitted pathogen of significant importance to tomato production around the globe. Synergistic interaction with pepino mosaic virus (PepMV), another seed and mechanically transmitted virus, and long-distance dissemination of these two viruses via contaminated tomato fruits through global marketing were previously suggested. In 2019, we detected both viruses in several grocery store-purchased tomatoes in South Florida, USA. In this study, to identify potential sources of inoculum, co-infection status, prevalence, and genomic diversity of these viruses, we surveyed symptomatic and asymptomatic imported tomatoes sold in ten different groceries in four cities in South Florida. According to the product labels, all collected tomatoes originated from Canada, Mexico, or repacking houses in the United States. With high prevalence levels, 86.5% of the collected samples were infected with ToBRFV, 90% with PepMV alone, and 73% were mixed-infected. The phylogenetic study showed no significant correlations between ToBRFV genomic diversity and the tomato label origin. Phylogenetic analysis of PepMV isolates revealed the prevalence of the PepMV strains, Chilean (CH2) and recombinant (US2). The results of this study highlight the continual presence of PepMV and ToBRFV in imported tomatoes in Florida grocery stores. [ABSTRACT FROM AUTHOR]

No other material in the world around us can equal bacterial cellulose in terms of the application scope. This is a kind of unique material that gained popularity in the 20th century and provided an excellent target of scientific inquiry. Its production and practical application in various areas of human activity are currently of much importance. Also, a broad range of research into the basic principles of bacterial cellulose production revealed many promising applications for food industry byproducts as energy sources for its growth, which makes this material more environmentally friendly than its plant-derived analog. Despite rich research and production history, bacterial cellulose is still considered to be a not fully studied material. This allows researchers to identify new energy sources for the growth of bacterial cellulose, to improve its quality, and to increase its quantity both on laboratory and industrial scales, and also to expand the application of bacterial cellulose to areas where, it would seem, it does not belong. In the modern scientific world, bacterial cellulose holds much promise as a matter for scientific inquiry and further technological applications. [ABSTRACT FROM AUTHOR]

The comprehensive management of organic urban solid waste is a concern due to its direct and indirect impact on the environment. Anaerobic Digestion (AD) has been recognized as an alternative and environmentally friendly technology for waste disposal, converting them into organic fertilizers and renewable energy. This research presents an experiment involving four reactors fed with household organic waste, three inoculated with canine, goat, and rabbit manure, and one without inoculum. The experiment was observed for 30 consecutive days to analyze the pH and temperature parameters involved in the AD process in domestic reactors. Statistical methodology, including one-way analysis of variance for assessing the effect of the type of inoculum, Tukey's simultaneous confidence intervals for mean differences, and 90 % confidence intervals for μ in temperature and manure, was utilized. Additionally, main effects analysis of the factors of average temperature and pH were conducted. The results of the one-factor experiment show that the type of inoculum does not significantly influence the variation in pH, while temperature remains relatively stable throughout the AD process. However, the analysis of main effects indicates that goat manure tends to stabilize the temperature with minimal variation, whereas variation is more heterogeneous in the other experiments.

This study aims to enhance biodegradation in WSP through the integrated application of thermal coating and inoculation. The integrated-coated concrete ponds (ICCPs) were designed as single-cell units of different aspect ratios and operated as a discontinuous-flow system. Settled sewage was first uniformly dosed in a constant head tank with mixed mesotrophic bacteria culture at 1.7 %, 3.3 %, and 5.0 % volume/volume ratio before being treated in the ponds. The result showed that it takes ten days to reduce BOD to 20 mg/L and 6 mg/L in the control pond and ICCP respectively. The heat energy causes the water temperature to increase due to the thermal capacity of the coated concrete surface maintaining a temperature condition in the pond system. Hence, this study was able to bridge the gap in the daily fluctuation of temperatures which influences microbial activity. Therefore, the effluent of the novel design was found suitable for irrigation purposes.

Pineapple (Ananas comosus) is commonly infected by Fusarium oxysporum, causal agent of the fusarium wilt disease. Conventionally, growers use synthetic fungicides to control the disease, which lead to environmental pollution, hazardous effects on non-target organisms and risks on human health. The aim of this work was to assess the effectiveness of Bacillus subtilis ANT01 and Rhizobium sp. 11B to control fusarium wilt on pineapple plants. Four treatments derived from a complete factorial design were tested under field conditions. Treatments composed of B. subtilis ANT01 and the combination B. subtilis ANT01–Rhizobium sp. 11B decreased disease severity by 94.4% and 86.1%, respectively. On the other hand, the treatment prepared with Rhizobium sp. 11B alone showed a reduction of 75.0%. Size of leaves and nutritional condition (SPAD units) of the biocontrol agents-treated plants showed no statistical differences. Moreover, B. subtilis ANT01 decreased by 46% the initial soil population of F. oxysporum, while Rhizobium sp. 11B, B. subtilis ANT01 plus Rhizobium sp. 11B and control, showed a population reduction of 12.5%, 24.2% and 23.0%, respectively. These results make evident the potential of B. subtilis ANT01 as biocontrol agent of the pathogen under field conditions.

Restoring native plant species on degraded landscapes is challenging. Symbiotic partners in the plant rhizosphere can aid in nutrient acquisition, pathogen protection, stress tolerance, and many other processes. However, these microbes are often absent in altered landscapes and need to be re-integrated to improve restoration efforts. We evaluated, within a laboratory setting, the ability of commercial and indigenous rhizobia strains to form nodules on lupine species used for rangeland seedings in the Great Basin region of the Western United States and ascertained if these strains could be applied through a seed coating. We also evaluated if a compost amendment applied via seed coating could further enhance the performance of the rhizobia strains. Our analysis showed that successful nodulation could occur using commercial and wildland-collected indigenous strains through either a liquid culture applied to seedlings or as a dry seed coating. However, the number of root nodules and the presence of a pink color (indicating nitrogen fixation) were typically higher in the commercial product than in the indigenous strains. Compost did not improve nodulation or the performance of the nodules; however, this treatment alone improved shoot growth. Overall, these results suggest that commercial rhizobium may be more effective in improving plant growth, and future research with native rhizobia may want to consider identifying strains compatible with seed-coating delivery. Longer-term studies are now merited for assessing how the rhizobia strains evaluated in this study influence plant growth, particularly in a field setting.

Carbapenem-resistant Pseudomonas aeruginosa infections represent a critical public health concern, highlighting the need for the development of effective antibiotics. Cefiderocol demonstrated potent in vitro activity against Pseudomonas aeruginosa, particularly in strains that are resistant to other drugs. However, concerns regarding the emergence of drug-resistant strains persist. This study, conducted with 109 carbapenem-resistant Pseudomonas aeruginosa strains from the Spanish Hospital (Dr. Balmis, Alicante). The study evaluated susceptibility to cefiderocol in comparison to alternative antibiotics and including their susceptibility to bacterial inoculum, while assessing various testing methods. Our findings revealed high susceptibility to cefiderocol against carbapenem-resistant strains, with only 2 of 109 strains exhibiting resistance. Comparative analysis demonstrated superiority of cefiderocol towards alternative antibiotics. Both the E-test and disk-diffusion methods showed 100% concordance with the microdilution method in classifying strains as susceptible or resistant. However, 4.6% (5/109) of disc zone diameters fell within the technical uncertainty zone, so the E-test technique was found to be more useful in routine clinical practice. Additionally, escalating bacterial inoculum correlated with decreases in vitro activity, so this parameter should be adjusted very carefully in in vivo studies. This study underscores cefiderocol’s potential as a therapeutic option for carbapenem-resistant Pseudomonas aeruginosa infections. However, the emergence of drug-resistant strains emphasizes the critical need for a wise use of antibiotics and a continuous monitoring of resistance to antibiotics. Based on our in vitro data, further investigation concerning the impact of bacterial inoculum on drug efficacy is warranted in order to detect resistance mechanisms and optimize treatment strategies, thereby mitigating the risk of resistance.

Biological nitrogen fixation (BNF) can provide the necessary nitrogen for bean crops; however, for this to occur, important limitations involving the inoculant application technology need to be overcome.The use of co-inoculation is a management technique used to obtain benefits and increase the potential of N2 fixation from the association between bacteria from the rhizobia group, such as R. tropici, and bacteria that promote plant growth, such as A. brasilense, in association with the addition of nutrients that allow greater efficiency of bacteria fixing atmospheric N2. This study aimed to evaluate the bean response to the reinoculation of R. tropici in co-inoculation with A. brasilense in a mixture with the micronutrients Co/Mo, in the winter season of 2021, in Anápolis-GO, Brazil. A randomized block design was used, with four replications, and the following treatments (TRs) were studied: TR1—reinoculation with R. tropici; TR2—reinoculation with co-inoculation of R. tropici + A. brasilense; TR3—reinoculation of R. tropici + Mo/Co micronutrients; TR4—reinoculation with co-inoculation R. tropici + A. brasilense + Mo/Co micronutrients; TR5—inoculation via seed, without reinoculation; TR6—mineral N fertilization in the sowing furrow and topdressing; TR7—control, without any N source. At stage R6, nodulation characteristics (number and dry mass of nodules) and the morphophysiological parameters of the plants (main root length, root dry mass, plant height, shoot dry mass, leaf area, and leaf N content in the shoot) were evaluated. At harvest, the final plant stand and components (number of pods per plant, number of grains per pod, and average weight of one hundred grains) were determined, in addition to grain yield. It was concluded that inoculation followed by reinoculation in topdressing with R. tropici in co-inoculation with A. brasilense plus Mo/Co, compared to mineral nitrogen fertilization, improves the efficiency of the nodulation process and the morphophysiological characteristics of the common bean crop. Seed inoculation and topdressing application with R. tropici, associated with co-inoculation with A. brasilense + Mo and Co, have the potential to completely replace mineral nitrogen fertilization in common bean crops.

In the face of persistent soil degradation in Benin caused by poor agricultural practices, including excessive use of chemical fertilizers, it is urgent to find solutions that take into account the microorganisms of interest. This study aimed to assess the effect of combining three strains of indigenous arbuscular mycorrhizal fungi (AMF) on maize production in northern Benin. The study involved 34 growers in Ouénou, Bagou and Kokey. The experimental setup consisted of three elementary plots with three treatments. Growth parameters were measured every 15 days, from the 15th to the 60th day after sowing, on ten plants per plot. Plant nutritional status, grain yield and mycorrhization were measured. The results showed that biostimulant + 50% NPK_Urea (N = nitrogen, P = phosphorus and K = potassium) had similar positive effects on growth parameters to those induced by the application of 100% NPK_Urea. Gains of 30.25% to 36.35% were recorded in plant height at Kokey. On the other hand, biostimulant+ 50% NPK_Urea induced a better phosphorus uptake of 21.08% to 27.77%. In addition, the grain yield of mycorrhizal plants was 8.37% higher than that of plants receiving 100% NPK_Urea at Ouénou. These results show that this technology could be integrated into the agricultural system to promote sustainable maize growing in Benin.

Papaya (Carica papaya L.) is a climacteric fruit with a resilient and distinctive aroma. The consumption of the fruit is global due to its high nutritive and medicinal values. However, there has been low production of quality papaya fruits due to unavailability of crucial mineral elements in the soils. The current study therefore, focuses on the effect of indigenous arbuscular mycorrhizal fungi (AMF) as a biofertilizer on the quality of papaya fruits. In order to verify AMF effectiveness on papaya fruits, four treatments were used: AMF inoculum only, composted farm yard manure (FYM) only, combination of AMF inoculum and compost FYM and control where only soil and sand media were used at a ratio of 1:1. Jomo Kenyatta University of Agriculture and Technology (JKUAT) and Malkia papaya hybrids were used. The papaya seeds from JKUAT and Malkia papaya hybrids were sown in trays and transplanted at 3 leaves stage into 5 litre pots within a green house. The AMF spores were bulked using sorghum plants to obtain the AMF inoculum. The treatments were added into the soil media of the papaya plantlets at a ratio of 1:3, every 4 weeks after first transplanting until they were 20 weeks old. They were then transplanted to 100 litre containers, where completely randomized design was used and replication of six papaya plants for each treatment and hybrid. Watering, weeding and cooling the green house with water fumes was carried out when necessary; as the papaya plants grew until the fruits attained physiological maturity. The fruits were separately harvested and ripened to a predetermined stage. They were then analysed for moisture content, crude fibre, minerals (nitrogen, phosphorous, potassium, magnesium, calcium, iron and zinc), ascorbic acid, total carotenoids and total polyphenols. Data obtained was subjected to twoway ANOVA at p = 0.05 significance level; means were separated using Tukey's HSD test in Genstat's 15th edition. JKUAT hybrid with AMF inoculum treatment had 3.07%crude fibre and 8.42mg/100g phosphorous content while JKUAT hybrid with both AMF inoculum and manure treatments had 4.9 % crude fibre and 9.88 mg/100g phosphorous content. Malkia and JKUAT hybrids with compost FYM treatment had potassium content of 98.31mg/100g and 109.4 mg/100g respectively while the controls had 31.58 mg/100g and 35.32mg/100g respectively. Incorporating soil media with manure and AMF inoculum improved the nutritive quality of papaya fruits and this was contingent on papaya hybrids. [ABSTRACT FROM AUTHOR]

The application of biochar as an additive to enhance the anaerobic digestion (AD) of biomass has been extensively studied from various perspectives. This study reported, for the first time, the influence of biochar incubation in the inoculum on the anaerobic fermentation of glucose in a batch-type reactor over 20 days. Three groups of inoculum with the same characteristics were pre-mixed once with biochar for different durations: 21 days (D21), 10 days (D10), and 0 days (D0). The BC was mixed in the inoculum at a concentration of 8.0 g/L. The proportion of the inoculum and substrate was adjusted to an inoculum-to-substrate ratio of 2.0 based on the volatile solids. The results of the experiment revealed that D21 had the highest cumulative methane yield, of 348.98 mL, compared to 322.66, 290.05, and 25.15 mL obtained from D10, D0, and the control, respectively. Three models—modified Gompertz, first-order, and Autoregressive Integrated Moving Average (ARIMA)—were used to interpret the biomethane production. All models showed promising fitting of the cumulative biomethane production, as indicated by high R 2 and low RMSE values. Among these models, the ARIMA model exhibited the closest fit to the actual data. The biomethane production rate, derived from the modified Gompertz Model, increased as the incubation period increased, with D21 yielding the highest rate of 31.13 mL/gVS. This study suggests that the application of biochar in the anaerobic fermentation of glucose, particularly considering the short incubation period, holds significant potential for improving the overall performance of anaerobic digestion. [ABSTRACT FROM AUTHOR]

Stevia rebaudiana Bertoni is a perennial species of high commercial value for its medicinal properties. In order to determine the effect of the inoculation of efficient strains of arbuscular mycorrhizal fungi in stevia cuttings, this research was carried out under semi-controlled conditions at the National Institute of Agricultural Sciences. Disease-free cuttings were used, with a length of 10 cm and with five pairs of leaves for each one. Three AMF strains were studied: Rhizoglomus irregulare, Funneliformis mosseae and Glomus cubense, in addition to a control without inoculation, in the first experiment. For the second experiment, the strain with the best performance from the previous test was used, in addition to two combinations of substrate (soil+organic fertilizer). Treatments were distributed in a completely randomized design with three repetitions and 20 plants for each one. Evaluations were carried out at the end of the nursery stage of the crop and variables related to the fungal function and the growth and development of plants were analyzed. Results demonstrated the AMF inoculation effectiveness on the variables related to the growth and development of stevia cuttings at 40 days after planting. Rhizoglomus irregulare strain showed a better performance under the experimental conditions evaluated. [ABSTRACT FROM AUTHOR]

Acidogenic fermentation is an emerging biotechnology that allows for the utilization of food waste as a feedstock to produce high-value products such as short-chain fatty acids (SCFAs), effectively offering a tangible solution for food waste management as well as resource recovery. The objectives of the current study were to identify the ideal inoculum, waste-activated sludge (WAS) or anaerobic digester sludge (AD), for the acidogenic fermentation of food waste at room temperature, as well as to evaluate the impact of heat pretreatment of these inoculums on fermentation performance. The maximum hydrolysis yield of 399 g sCOD/kg VS added was obtained when untreated AD was used as the inoculum, whereas the pretreated AD inoculum provided the highest SCFA yield and conversion efficiency of 238 g sCODSCFA/kg VS added and 71%, respectively. Heat pretreatment had a detrimental impact on the WAS inoculum, leading to lower hydrolysis and SCFA yields, but exerted a positive influence on the AD inoculum. The microbial community showed that heat pretreatment negatively impacted the abundance of non-spore-forming hydrolytic and acidogenic microorganisms. Overall, this study demonstrates the critical role of inoculum type and heat pretreatment in optimizing the acidogenic fermentation process, laying the groundwork for future refinements in SCFA production from food waste through inoculum design.

Lignocellulosic is a carbon source biomass composed of cellulose, hemicelluloses, and lignin, which are strongly associated with each other. This fact makes them hardly degradable by produced microbial enzymes when introduced to compost piles. To address this problem, a primary single or combined pretreatment method of this biomass allows for the separation of these complex, interlinked fractions, allowing a better accessibility for microbial enzymes. However, the rugged lignin component, in addition to several produced by products from these pretreatments, inhibit the microbial activity. For this, the optimization of these treatments with other interfering parameters is the base for a successful composting process. In this work, nine compost piles were initiated, in which their lignocellulosic fraction was subjected to chemical and microbiological treatments alone or combined while preserving a control. The obtained results showed that the combined pre-treatment of the primary organic raw materials with 10% NaOH and adapted microbial inoculum at 2.5% was the best suited for compost piles in Mediterranean regions. This treatment ensured the quickening of the composting process by 15 days, while yielding a final compost of a higher quality in regard to its physic-chemical characteristics, especially its C:N and CC values. Furthermore, it ensured a higher sanitation through the elimination of different microbial pathogens from the final compost, by means of the secondary metabolites produced by the microbial adapted consortia. This 'tailor-made' process could be replicated for the treatment of other generated sources of organic raw materials within the Mediterranean region. [ABSTRACT FROM AUTHOR]

Yoghurt was produced from fresh cow milk inoculated with baobab pulp powder. Baobab pulp powder was added at 0.34%, 0.52%, 0.69%, and 0.85% respectively, while the control sample (A) was yoghurt produced from fresh cow milk inoculated with regular commercial starter culture. The physical, chemical, microbiological, anti-nutritional and sensory properties of the samples were analysed using standard procedures. The moisture content, crude protein and fat content decreased with the added baobab pulp powder, while the fibre, ash and carbohydrate contents increased. The pH ranged from 4.34 to 4.74, coupled with negligible anti-nutritional composition. The titratable acidity increased with added baobab pulp powder from 0.52-0.69%. Brix and viscosity of treated samples increased respectively from 30.85-40.02 and 200.05-200.14. Total bacterial and fungi counts ranged from 8.65×104 to 15.51×104 Cfu/mL and 1.12×104 to 4.54×104 Cfu/mL respectively, with the control sample having the higher loads. The over-all acceptability of the samples were significant (p>0.05); sample E (0.85% inoculation) was the most preferred, followed by sample B (0.34% inoculation), while the least accepted sample was the control. Inoculating milk with baobab pulp powder produced yoghurt with improved and acceptable qualities. [ABSTRACT FROM AUTHOR]

Cereals are a major component in the rations of ruminants and their management in it has a significant role. The aim of the study is to determine the influence of the variety and cultivation technologies of wheat, barley and corn on gas production, digestibility and metabolic energy through in vitro methodologies in fresh and lyophilized inoculum. By means of a probe and a vacuum machine we collect inoculum (rumen fluid) from rams 2 - 3 years old from the breed Bulgarian dairy synthetic population (BDSP). An in vitro methodology was used to perform analyzes of rough and succulent feed's with the Ankom RF Gas Production System. Digestibility and metabolic energy was evaluated and gas production was analyzed at four levels: 24, 48, 72 and 120 hours of incubation, with fresh and lyophilized rumen fluid. Determined energy value with fresh rumen fluid in these varieties of corn is 10 - 16% higher than in lyophilization. The trend is similar for barley. In the case of wheat varieties, the lowest levels of the total amount of released gases in both types of inoculum (fresh and lyophilized) were found at 24 h incubation. At 48 and 72 hours these quantities increase, the most significant being in the variety Miryana - from 130.20 dm/mL to 195.41 dm/mL at 24 and 48 hours, and the least in the variety Sobel - 160.30 dm/mL and 176.01 dm/mL respectively. In the case of barley, the increase in gas production is more significant at 48 hours compared to 24 hours, and insignificant at 72 hours compared to 48 hours. For Krasi variety this increase is the smallest - from 147.38 dm/mL to 172.30 dm/mL at 24 and 72 hours respectively. The most significant increase was in gas production in the Obzor variety - from 117.88 dm/mL to 170.38, respectively. The data show lower emissions of maize using lyophilised inoculum at the three incubation levels compared to fresh. This trend is maintained in all four varieties of corn. Digestibility in different wheat varieties is high with close values of lyophilized and fresh rumen fluid. The trend is similar for barley and corn. The established metabolic energy is higher in all wheat varieties with lyophilized inoculum compared to fresh. [ABSTRACT FROM AUTHOR]

The efficiency of the natural symbiotic relationship between communally produced legumes and nitrogen fixing bacteria has been reported to be very poor, mainly due to poor soils in communal farming areas. With the use of synthetic fertilizers to increase soil fertility being both environment-unfriendly and unaffordable for majority of communal farmers, strategies that are cost-effective and climate smart need to be put in place to improve the productivity of communal legumes such as Bambara groundnuts. A 5 x 3 factorial arrangement experiment in a randomised complete block design with five replications was established under greenhouse conditions. Factors consisted of bacterial species: [Bradyrhizobium japonicum (Bj), Rhizobium loti (Rl), Rhizobium meliloti (Rm), Rhizobium leguminosarum biovar phaseoli (Rlt) and a negative control (untreated)], and local Bambara groundnut varieties: black, creamy white-eye, and red. Bambara groundnut seeds were inoculated with levels recommended for cowpea and soyabean. Nitrogen fixing bacteria application on Bambara groundnuts increased plant growth and nodulation variables, with the effects varying with Bambara groundnut variety and rhizobium type. Bj outperformed the other tested bacteria on nodulation variables, with the red cultivar performing better in all growth variables, making it suitable for pod and vegetative production. [ABSTRACT FROM AUTHOR]