164 results on '"Institut Pasteur du Laos"'
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2. A Six Years (2010–2016) Longitudinal Survey of the Four Serotypes of Dengue Viruses in Lao PDR
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Charlotte Balière, Elodie Calvez, Jean-Michel Thiberge, Somphavanh Somlor, Mathias Vandenbogaert, Marc Grandadam, Valérie Caro, Environnement et Risques infectieux - Environment and Infectious Risks (ERI), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), This research was funded by UNITEDengue and Global Partnership Program, Canada (ASEAN-GPP Grant Phase 3—Laboratory Capacity Development for diagnostics of Emerging Dangerous Pathogens) and Actions Concertées Inter-Pasteuriennes (ACIP) A09-2014., and We thank all the medical staff of the hospital network in Vientiane Capital and the different provinces for their active and motivated participation in the surveillance of dengue. We also thank the Arbovirus and Emerging Viral diseases Laboratory staff of Institut Pasteur du Laos for the biological analysis. We thank Paul T. Brey, Director of Institut Pasteur du Laos, for his support throughout the UnitedDengue Project. We thank Véronique Hourdel (Institut Pasteur, Environment and Infectious Risks Unit, Paris, France) for her help in phylogenetic analyses.
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Microbiology (medical) ,Lao PDR ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Virology ,DENV-2 ,dengue ,DENV-1 ,DENV-3 ,DENV-4 ,envelope gene ,phylogeny ,Microbiology - Abstract
International audience; Dengue fever is the most prevalent arthropod-borne viral infection of humans in tropical and subtropical countries. Since 1979, dengue has been reported to be endemic in the Lao People’s Democratic Republic (PDR), as in many countries in Southeast Asia, with a complex circulation of the four dengue viruses’ serotypes (DENV-1 to DENV-4). By sequencing the complete envelope protein, we explored a panel of samples from five Lao Provinces (Vientiane capital, Luangprabang, Bolikhamxay, Saravane, Attapeu) to enrich knowledge about the co-circulation of DENVs in Lao PDR between 2010 and 2016. Phylogenetic analyses highlighted the specific circulation of DENV-1 genotype I, DENV-2 genotype Asian I, DENV-4 genotype I and the co-circulation of DENV-3 genotype II and III. The continuous co-circulation of the four serotypes was underlined, with genotype or cluster shifts among DENV-3 and DENV-1. These data suggested the emergence or re-emergence of DENV strains associated with epidemic events, potentially linked to the exchanges within the territory and with neighboring countries. Indeed, the increasing local or regional connections favored the dissemination of new isolates or new clusters around the country. Since 2012, the surveillance and alert system created in Vientiane capital by the Institut Pasteur du Laos appears to be a strategic tool for monitoring the circulation of the four serotypes, especially in this endemic country, and allows for improving dengue epidemiological knowledge to anticipate epidemic events better.
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- 2023
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3. Trends of the dengue serotype-4 circulation with epidemiological, phylogenetic, and entomological insights in Lao PDR between 2015 and 2019
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Phaithong Bounmany, Thonglakhone Xaybounsou, Marc Choisy, Sitsana Keosenhom, Julien Pompon, Elodie Calvez, Marc Grandadam, Thep Aksone Chindavong, Paul T. Brey, Virginie Pommelet, Phoyphaylinh Prasayasith, Somphavanh Somlor, Souksakhone Viengphouthong, Sébastien Marcombe, Olivier Telle, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Duke-NUS Medical School [Singapore], Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre de sciences humaines de New Delhi (CSH), Ministère de l'Europe et des Affaires étrangères (MEAE)-Centre National de la Recherche Scientifique (CNRS), Centre for Policy Research [New Delhi] (CPR), Nuffield Department of Medicine [Oxford, UK] (Big Data Institute), University of Oxford [Oxford], Oxford University Clinical Research Unit [Ho Chi Minh City] (OUCRU), Institut de Recherche Biomédicale des Armées (IRBA), This work was funded by the Agence Française de Développement grant n° CZZ 2146 01 (Ecomore2 project) and by UNITEDengue and Global Partnership Programme, Canada (ASEAN-GPP Grant Phase 3—Laboratory Capacity Development for diagnostics of Emerging Dangerous Pathogens)., We thank Lee Ching Ng, Director, Environmental Health Institute (EHI), National Environment Agency, Chanditha Hapuarachchi, and Carmen Koo for their sequencing technical assistance. We also thank the Institut Pasteur du Laos staff for the mosquito collections and identification (Phoutmany Thammavong, Phonesavanh Luangamath, Kaithong Lakeomany, Somphat Nilaxay, and Vaekey Vungkyly)., Géographie-cités (GC (UMR_8504)), Université Paris 1 Panthéon-Sorbonne (UP1)-École des hautes études en sciences sociales (EHESS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), University of Oxford, and Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA)
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Microbiology (medical) ,Serotype ,medicine.medical_specialty ,Aedes vectors ,viruses ,030231 tropical medicine ,lcsh:Medicine ,PARIS team ,phylogeny ,Arbovirus ,Article ,epidemic ,Dengue fever ,03 medical and health sciences ,0302 clinical medicine ,Lao PDR ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Epidemiology ,medicine ,Immunology and Allergy ,Socioeconomics ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,General Immunology and Microbiology ,Phylogenetic tree ,ACL ,lcsh:R ,Significant difference ,virus diseases ,Outbreak ,DENV-4 ,Integrated approach ,medicine.disease ,dengue ,3. Good health ,Infectious Diseases ,Geography - Abstract
Dengue outbreaks have regularly been recorded in Lao People&rsquo, s Democratic Republic (PDR) since the first detection of the disease in 1979. In 2012, an integrated arbovirus surveillance network was set up in Lao PDR and an entomological surveillance has been implemented since 2016 in Vientiane Capital. Here, we report a study combining epidemiological, phylogenetic, and entomological analyzes during the largest DENV-4 epidemic ever recorded in Lao PDR (2015&ndash, 2019). Strikingly, from 2015 to 2019, we reported the DENV-4 emergence and spread at the country level after two large epidemics predominated by DENV-3 and DENV-1, respectively, in 2012&ndash, 2013 and 2015. Our data revealed a significant difference in the median age of the patient infected by DENV-4 compared to the other serotypes. Phylogenetic analysis demonstrated the circulation of DENV-4 Genotype I at the country level since at least 2013. The entomological surveillance showed a predominance of Aedesaegypti compared to Aedesalbopictus and high abundance of these vectors in dry and rainy seasons between 2016 and 2019, in Vientiane Capital. Overall, these results emphasized the importance of an integrated approach to evaluate factors, which could impact the circulation and the epidemiological profile of dengue viruses, especially in endemic countries like Lao PDR.
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- 2021
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4. Low seroprevalence of COVID-19 in Lao PDR, late 2020
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Darouny Phonekeo, Thierry Rose, Sengchanh Kounnavong, Somphavanh Somlor, Nicolas Escriou, Khamsing Vongphayloth, Somphou Sayasone, Mayfong Mayxay, Vilaysone Khounvisith, Phonepadith Xangsayarath, Siriphone Virachith, Antony P. Black, Paul T. Brey, Elodie Calvez, Marc Eloit, Virginie Pommelet, Vincent Lacoste, Judith M. Hübschen, Sarah Temmam, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Lao Tropical Public Health Institute, Institute of Research and Education Development [Vientiane, Laos] (IRED), University of Health Sciences [Vientiane, Laos] (UHS)-Ministry of Health [Laos], Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Mahidol University [Bangkok]-Mahosot Hospital, Centre for Tropical Medicine and Global Health [Oxford, UK], Nuffield Department of Medicine [Oxford, UK] (Big Data Institute), University of Oxford-University of Oxford, National Centre for Laboratory and Epidemiology [Vientiane, Laos], Découverte de pathogènes – Pathogen discovery, Institut Pasteur [Paris] (IP), Département de Santé Globale - Department Global Health, Biologie Cellulaire des Lymphocytes - Lymphocyte Cell Biology, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Luxembourg Institute of Health (LIH), Agence Française de Développement. Additional, Institut Pasteur du Laos, Institute Pasteur, Paris and Luxembourg Ministry of Foreign and European Affairs ('PaReCIDS II')., and TEMMAM, Sarah
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[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Health Policy ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Public Health, Environmental and Occupational Health ,Obstetrics and Gynecology ,Age and sex ,Article ,Psychiatry and Mental health ,Infectious Diseases ,[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Pediatrics, Perinatology and Child Health ,Internal Medicine ,Medicine ,Seroprevalence ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Christian ministry ,Geriatrics and Gerontology ,Public aspects of medicine ,RA1-1270 ,business ,Demography - Abstract
Background In 2020 Lao PDR had low reported COVID-19 cases but it was unclear whether this masked silent transmission. A seroprevalence study was done August - September 2020 to determine SARS-CoV-2 exposure. Methods Participants were from the general community (n=2433) or healthcare workers (n=666) in five provinces and bat/wildlife contacts (n=74) were from Vientiane province. ELISAs detected anti- SARS-CoV-2 Nucleoprotein (N; n=3173 tested) and Spike (S; n=1417 tested) antibodies. Double-positive samples were checked by IgM/IgG rapid tests. Controls were confirmed COVID-19 cases (n=15) and pre-COVID-19 samples (n=265). Seroprevalence for the general community was weighted to account for complex survey sample design, age and sex. Findings In pre-COVID-19 samples, 5·3%, [95% CI=3·1-8·7%] were anti-N antibody single-positive and 1·1% [0·3-3·5%] were anti-S antibody single positive. None were double positive. Anti-N and anti-S antibodies were detected in 5·2% [4·2-6·5%] and 2·1% [1·1-3·9%] of the general community, 2·0% [1·1-3·3%] and 1·4% [0·5-3·7%] of healthcare workers and 20·3% [12·6-31·0%] and 6·8% [2·8-15·3%] of bat/wildlife contacts. 0·1% [0·02-0·3%] were double positive for anti-N and anti-S antibodies (rapid test negative). Interpretation We find no evidence for significant SARS-CoV-2 circulation in Lao PDR before September 2020. This likely results from early decisive measures taken by the government, social behavior, and low population density. High anti-N /low anti-S seroprevalence in bat/wildlife contacts may indicate exposure to cross-reactive animal coronaviruses with threat of emerging novel viruses. Funding Agence Française de Développement. Additional; Institut Pasteur du Laos, Institute Pasteur, Paris and Luxembourg Ministry of Foreign and European Affairs (“PaReCIDS II”).
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- 2021
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5. First probable case of congenital Zika syndrome in Lao People’s Democratic Republic
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Souksakhone Viengphouthong, Phommady Vetsaphong, Somphavanh Somlor, Virginie Pommelet, Elodie Calvez, Myrielle Dupont-Rouzeyrol, Paul T. Brey, Thonglakhone Xaybounsou, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Mother and Child Hospital [Vientiane, Laos], Institut Pasteur de Nouvelle-Calédonie, The work was supported by the Institut Pasteur du Laos., and We thank the Neonatal Intensive Care Unit of the Mother and Child Hospital in Vientiane capital city for their clinical support. We also thank the Arbovirus and Emerging Viral Diseases Laboratory of the Institut Pasteur du Laos for technical support.
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0301 basic medicine ,Microbiology (medical) ,Microcephaly ,Probable Case ,030106 microbiology ,LAO PEOPLE'S DEMOCRATIC REPUBLIC ,education ,MESH: Asia, Southeastern ,MESH: Zika Virus ,Infectious and parasitic diseases ,RC109-216 ,MESH: Microcephaly ,Zika virus ,03 medical and health sciences ,0302 clinical medicine ,Lao PDR ,MESH: Pregnancy ,MESH: Zika Virus Infection ,medicine ,ZikV Infection ,MESH: Animals ,030212 general & internal medicine ,MESH: Disease Outbreaks ,Congenital Zika syndrome ,Aedes ,MESH: Humans ,biology ,MESH: Infant, Newborn ,Outbreak ,MESH: Adult ,General Medicine ,MESH: Aedes ,biology.organism_classification ,medicine.disease ,Virology ,MESH: Male ,3. Good health ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Flavivirus ,Infectious Diseases ,Geography ,MESH: Laos ,MESH: Female - Abstract
International audience; Zika virus (ZIKV) is a Flavivirus transmitted by Aedes mosquitoes, and was responsible for a worldwide outbreak between 2013 and 2016. However, no ZIKV outbreak has been described in Southeast Asia since 2017. In this study, we report the first microcephaly case with probable ZIKV infection during pregnancy in Lao People's Democratic Republic.
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- 2021
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6. Description of a new species of Dermacentor Koch, 1844 (Acari: Ixodidae) from the mountains of Laos and Thailand
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Wachareeporn Trinachartvanit, Dmitry A. Apanaskevich, Maria A. Apanaskevich, Aummarin Chaloemthanetphong, Paul T. Brey, Arunee Ahantarig, Pattraporn Jeangkhwoa, Khaithong Lakeomany, Khamsing Vongphayloth, Georgia Southern University, University System of Georgia (USG), Russian Academy of Sciences [Moscow] (RAS), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Mahidol University [Bangkok], and This project was partially supported by Mahidol University and BDC PERDO (BDC-PG3-161006), Thailand. This study in Laos was funded by Institut Pasteur du Laos.
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0106 biological sciences ,Dorsum ,Male ,Sus scrofa ,010607 zoology ,Zoology ,01 natural sciences ,030308 mycology & parasitology ,03 medical and health sciences ,Wild boar ,Species Specificity ,biology.animal ,medicine ,MESH: Species Specificity ,Animals ,Acari ,MESH: Animals ,MESH: Thailand ,Dermacentor ,0303 health sciences ,biology ,biology.organism_classification ,Thailand ,MESH: Male ,MESH: Sus scrofa ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,MESH: Laos ,Animal ecology ,Laos ,MESH: Dermacentor ,Parasitology ,Female ,medicine.symptom ,Vegetation (pathology) ,MESH: Female ,Ixodidae - Abstract
International audience; Dermacentor pasteuri n. sp. (Acari: Ixodidae) is described based on adults ex wild boar and vegetation from Laos and Thailand. Adults of D. pasteuri n. sp. are similar to those of D. compactus Neumann, 1901, but can be distinguished by the shape of conscutum in the male, development of dorsal cornua in the female, size and shape of spurs on coxae and coloration of leg segments in both sexes.
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- 2019
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7. Risk of arbovirus emergence via bridge vectors: case study of the sylvatic mosquito Aedes malayensis in the Nakai district, Laos
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Stéphanie Dabo, Catherine Oke, Elodie Calvez, Louis Lambrechts, Elliott F. Miot, James G. Logan, Paul T. Brey, Fabien Aubry, Sébastien Marcombe, Marc Grandadam, Interactions Virus-Insectes - Insect-Virus Interactions (IVI), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Sorbonne Université (SU), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), London School of Hygiene and Tropical Medicine (LSHTM), This work was funded by the Institut Pasteur du Laos, the Institut Pasteur’s International Division (ACIP 2016-16), the Agence Nationale de la Recherche (grant ANR-17-ERC2-0016-01), the French Government’s Investissement d’Avenir program Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID), the City of Paris Emergence(s) program in Biomedical Research, and a JICA/AMED SATREPS project for 'the development of innovative research technique in genetic epidemiology of malaria and other parasitic diseases in the Lao PDR for containing their expanding endemicity.' The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication., ANR-17-ERC2-0016,GxG,Base génétique de la spécificité génotype-génotype dans l'interaction naturelle entre un virus et son insecte vecteur(2017), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
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0301 basic medicine ,Risk ,Conservation of Natural Resources ,[SDV]Life Sciences [q-bio] ,viruses ,030231 tropical medicine ,Population ,lcsh:Medicine ,Zoology ,Aedes aegypti ,Mosquito Vectors ,Dengue virus ,medicine.disease_cause ,Arbovirus ,Article ,03 medical and health sciences ,0302 clinical medicine ,Species Specificity ,Aedes ,medicine ,Animals ,Humans ,lcsh:Science ,education ,Ecological epidemiology ,education.field_of_study ,Multidisciplinary ,biology ,lcsh:R ,Yellow fever ,virus diseases ,biology.organism_classification ,Blood feeding ,medicine.disease ,Aedes malayensis ,3. Good health ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,030104 developmental biology ,Olfactometer ,Laos ,Viral infection ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Odorants ,lcsh:Q ,Arboviruses - Abstract
Many emerging arboviruses of global public health importance, such as dengue virus (DENV) and yellow fever virus (YFV), originated in sylvatic transmission cycles involving wild animals and forest-dwelling mosquitoes. Arbovirus emergence in the human population typically results from spillover transmission via bridge vectors, which are competent mosquitoes feeding on both humans and wild animals. Another related, but less studied concern, is the risk of ‘spillback’ transmission from humans into novel sylvatic cycles. We colonized a sylvatic population of Aedes malayensis from a forested area of the Nakai district in Laos to evaluate its potential as an arbovirus bridge vector. We found that this Ae. malayensis population was overall less competent for DENV and YFV than an urban population of Aedes aegypti. Olfactometer experiments showed that our Ae. malayensis colony did not display any detectable attraction to human scent in laboratory conditions. The relatively modest vector competence for DENV and YFV, combined with a lack of detectable attraction to human odor, indicate a low potential for this sylvatic Ae. malayensis population to act as an arbovirus bridge vector. However, we caution that opportunistic blood feeding on humans by sylvatic Ae. malayensis may occasionally contribute to bridge sylvatic and human transmission cycles.
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- 2019
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8. Population genetics of Aedes albopictus (Diptera: Culicidae) in its native range in Lao People’s Democratic Republic
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Elliott F. Miot, Maysa T Motoki, Phoutmany Thammavong, Bruna Demari-Silva, Paul T. Brey, Pattamaporn Kittayapong, Nothasine Phommavanh, Dina M. Fonseca, Sébastien Marcombe, Somsanith Chonephetsarath, Jeffrey C. Hertz, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Vysnova Partners, Inc. [Landover, MD], Smithsonian Institution, Rutgers, The State University of New Jersey [New Brunswick] (RU), Rutgers University System (Rutgers), Cellule Pasteur UPMC, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris], Interactions Virus-Insectes - Insect-Virus Interactions (IVI), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Universidade de São Paulo (USP), Naval Medical Research Unit Two [Singapore] (NAMRU-2), Naval Medical Research, Mahidol University [Bangkok], The views expressed in this publication are those of the author and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the U.S. Government. This study was partially supported by the U.S. Naval Medical Research Unit TWO, work unit number D1428, in support of the Military Infectious Diseases Research Program and Institut Pasteur du Laos. I (JCH) am a military service member. This work was prepared as part of my official duties. Title 17, U.S.C., §105 provides that copyright protection under this title is not available for any work of the U.S. Government. Title 17, U.S.C., §101 defines a U.S. Government work as a work prepared by a military service member or employee of the U.S. Government as part of that person’s official duties., Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Universidade de São Paulo = University of São Paulo (USP)
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0301 basic medicine ,MESH: Sequence Analysis, DNA ,Mosquito Control ,viruses ,MESH: Asia, Southeastern ,Population genetics ,cox1 gene ,Gene flow ,0302 clinical medicine ,Aedes ,Cluster Analysis ,MESH: Animals ,MESH: Genetic Variation ,MESH: Phylogeny ,Asia, Southeastern ,Phylogeny ,MESH: Mosquito Control ,education.field_of_study ,biology ,Asia, Eastern ,Ecology ,MESH: DNA ,MESH: Aedes ,Aedes albopictus ,Mitochondria ,Infectious Diseases ,Italy ,Laos ,Genetic structure ,MESH: Tropical Climate ,Female ,MESH: Mosquito Vectors ,Algorithms ,Genetic population ,MESH: Mitochondria ,MESH: Bayes Theorem ,030231 tropical medicine ,Population ,MESH: Genetics, Population ,MESH: Algorithms ,Mosquito Vectors ,lcsh:Infectious and parasitic diseases ,Electron Transport Complex IV ,03 medical and health sciences ,Lao PDR ,MESH: Electron Transport Complex IV ,MESH: United States ,Animals ,lcsh:RC109-216 ,Genetic variability ,education ,Isolation by distance ,Tropical Climate ,Research ,fungi ,Genetic Variation ,MESH: Italy ,Bayes Theorem ,DNA ,Sequence Analysis, DNA ,MESH: Haplotypes ,15. Life on land ,biology.organism_classification ,MESH: Far East ,MESH: Cluster Analysis ,United States ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Genetics, Population ,030104 developmental biology ,Haplotypes ,MESH: Laos ,Parasitology ,MESH: Female - Abstract
BackgroundThe Asian tiger mosquito,Aedes(Stegomyia)albopictus(Skuse) is an important worldwide invasive species and can be a locally important vector of chikungunya, dengue and, potentially, Zika. This species is native to Southeast Asia where populations thrive in both temperate and tropical climates. A better understanding of the population structure ofAe. albopictusin Lao PDR is very important in order to support the implementation of strategies for diseases prevention and vector control. In the present study, we investigated the genetic variability ofAe. albopictusacross a north-south transect in Lao PDR.MethodsWe used variability in a 1337-bp fragment of the mitochondrial cytochromecoxidase subunit 1 gene (cox1), to assess the population structure ofAe. albopictusin Lao PDR. For context, we also examined variability at the same genetic locus in samples ofAe. albopictusfrom Thailand, China, Taiwan, Japan, Singapore, Italy and the USA.ResultsWe observed very high levels of genetic polymorphism with 46 novel haplotypes inAe. albopictusfrom 9 localities in Lao PDR and Thailand populations. Significant differences were observed between the Luangnamtha population and other locations in Lao PDR. However, we found no evidence of isolation by distance. There was overall little genetic structure indicating ongoing and frequent gene flow among populations or a recent population expansion. Indeed, the neutrality test supported population expansion in LaotianAe. albopictusand mismatch distribution analyses showed a lack of low frequency alleles, a pattern often seen in bottlenecked populations. When samples from Lao PDR were analyzed together with samples from Thailand, China, Taiwan, Japan, Singapore, Italy and the USA, phylogenetic network and Bayesian cluster analysis showed that most populations from tropical/subtropical regions are more genetically related to each other, than populations from temperate regions. Similarly, most populations from temperate regions are more genetically related to each other, than those from tropical/subtropical regions.ConclusionsAedes albopictusin Lao PDR are genetically related to populations from tropical/subtropical regions (i.e. Thailand, Singapore, and California and Texas in the USA). The extensive gene flow among locations in Lao PDR indicates that local control is undermined by repeated introductions from untreated sites.
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- 2019
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9. New records and updated checklist of mosquitoes (Diptera: Culicidae) from Lao People's Democratic Republic, with special emphasis on adult and larval surveillance in Khammuane Province
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Maysa T Motoki, Leopoldo M. Rueda, Elliott F. Miot, Paul T. Brey, Khamsing Vongphayloth, Jeffrey C. Hertz, Alexandra Hiscox, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Smithsonian Institution, Cellule Pasteur UPMC, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris] (IP), Interactions Virus-Insectes - Insect-Virus Interactions (IVI), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Wageningen University and Research [Wageningen] (WUR), Naval Medical Research Unit Two [Singapore] (NAMRU-2), Naval Medical Research, This study was partially supported by the U.S. Naval Medical Research Unit TWO, work unit number D1428, in support of the Military Infectious Diseases Research Program and Institut Pasteur du Laos., Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris], and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
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Fauna ,030231 tropical medicine ,LAO PEOPLE'S DEMOCRATIC REPUBLIC ,Biodiversity ,Biology ,DNA barcoding ,03 medical and health sciences ,0302 clinical medicine ,Lao PDR ,Species Specificity ,parasitic diseases ,DNA barcode ,Animals ,MESH: Species Specificity ,MESH: Animals ,Laboratory of Entomology ,MESH: Phylogeny ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,030304 developmental biology ,0303 health sciences ,Larva ,Ecology ,Khammuane Province ,Cytochrome c oxidase subunit I ,MESH: Animal Distribution ,Forestry ,PE&RC ,Laboratorium voor Entomologie ,Checklist ,mosquito surveillance ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Culicidae ,MESH: Laos ,Laos ,mosquito species list ,Protected area ,MESH: Culicidae ,Animal Distribution ,MESH: Larva - Abstract
International audience; A list of mosquitoes from the Nakai Nam Theun National Protected Area along the Nam Theun, Nam Mon, Nam Noy, and Nam On rivers, Nakai District, Khammuane Province, Lao People's Democratic Republic (Lao PDR) is presented. Fifty-four mosquito taxa were identified, including 15 new records in the Lao PDR. A fragment of the mtDNA cytochrome c oxidase subunit I (COI) gene, barcode region, was generated for 34 specimens, and together with four specimens already published, it represented 23 species in eight genera. In addition, an updated checklist of 170 mosquito taxa from Lao PDR is provided based on field collections from Khammuane Province, the literature, and specimens deposited in the Smithsonian Institution, National Museum of Natural History (SI-NMNH), Washington, DC, U.S.A. This paper provides additional information about the biodiversity of mosquito fauna in Lao PDR.
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- 2019
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10. On the true identity of Sergentomyia gemmea and description of a closely related species : Se. raynali n. sp
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J. Depaquit, K. Vongphayloth, P. Siriyasatien, R. Polseela, A. Phumee, M. Loyer, A. Vol, G. Varlot, N. Rahola, P. T. Brey, I. W. Sutherland, J. C. Hertz, F. Gay, N. Léger, Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle (ESCAPE), Université de Reims Champagne-Ardenne (URCA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Centre Hospitalier Universitaire de Reims (CHU Reims), Medical Entomology and Biology of Disease Vectors [Vientiane, Lao], Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Chulalongkorn University [Bangkok], Naresuan University, Transmission Vectorielle et Épidémiosurveillance de Maladies Parasitaires - EA 4688 (VECPAR), SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), United States Navy - U.S. Navy (USA), Naval Medical Research Unit Two [Singapore] (NAMRU-2), Naval Medical Research, Sorbonne Université (SU), and This work was partially supported by the U.S. Naval Medical Research Unit TWO, work unit number D1428, in support of the Armed Forces Health Surveillance Branch – Global Emerging Infections Surveillance and Response System (AFHSB-GEIS) and the Institut Pasteur du Laos.
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Male ,Mitochondrial DNA ,Asia ,030231 tropical medicine ,Zoology ,Biology ,DNA, Mitochondrial ,030308 mycology & parasitology ,03 medical and health sciences ,taxonomy ,0302 clinical medicine ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,parasitic diseases ,Animals ,Ecology, Evolution, Behavior and Systematics ,molecular systematics ,[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health ,0303 health sciences ,General Veterinary ,Cytochrome b ,Sergentomyia gemmea ,Sequence Analysis, DNA ,Cytochromes b ,Thailand ,Leishmania martiniquensis - Phlebotomus ,Insect Vectors ,Sergentomyia ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Laos ,Insect Science ,GenBank ,Molecular phylogenetics ,Insect Proteins ,Parasitology ,Taxonomy (biology) ,Female ,Psychodidae ,Leishmania martiniquensis ,vector - Abstract
International audience; Several species of Leishmania are responsible for leishmaniases in Thailand, although little is known about their transmission. Sergentomyia gemmea has been suspected several times to transmit Leishmania martiniquensis. Some captures carried out in Thailand and Lao People's Democratic Republic have emphasized the scarcity of Se. gemmea, comprising only 1% of the collected females. The sequencing of cytochrome B mtDNA of our specimens showed that our specimens are not grouped with other Se. gemmea previously deposited in GenBank. The latter are grouped with some Se. khawi and Se. hivernus that we processed in the present study. We suspect misidentifications and propose focusing on the most useful characters for identification of Se. gemmea based on the examination of type-specimens. The examination of the ascoids exhibiting anterior spurs is the most important one. However, we also describe Se. raynali n. sp. exhibiting comparable spurs but differing from Se. gemmea by its original cibarium. Finally, the vectorial role of Se. gemmea appears very questionable in the absence of new evidence.
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- 2019
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11. Predicting the Effects of Climate Change on Dengue Vector Densities in Southeast Asia through Process-Based Modeling
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Lucas Bonnin, Annelise Tran, Vincent Herbreteau, Sébastien Marcombe, Sébastien Boyer, Morgan Mangeas, Christophe Menkes, Ecologie marine tropicale des océans Pacifique et Indien (ENTROPIE [Nouvelle-Calédonie]), Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Ifremer - Nouvelle-Calédonie, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Nouvelle-Calédonie (UNC), Territoires, Environnement, Télédétection et Information Spatiale (UMR TETIS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UMR 228 Espace-Dev, Espace pour le développement, Institut de Recherche pour le Développement (IRD)-Université de Perpignan Via Domitia (UPVD)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Guyane (UG)-Université des Antilles (UA)-Université de Montpellier (UM), Univ Réunion espace-dev IRD Univ Guyane Uiv Montpellier (Espace Dev), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur du Cambodge, and Agence Française de Développement (AFD)
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Risk ,Chikundunya ,albopictus skuse ,Aedes-aegypti diptera ,Culicidae ,Mosquito ,Fever ,Health, Toxicology and Mutagenesis ,Population ,[SDE]Environmental Sciences ,Public Health, Environmental and Occupational Health ,Temperature ,Driven abundance model - Abstract
Background: Aedes aegypti and Ae. albopictus mosquitoes are major vectors for several human diseases of global importance, such as dengue and yellow fever. Their life cycles and hosted arboviruses are climate sensitive and thus expected to be impacted by climate change. Most studies investigating climate change impacts on Aedes at global or continental scales focused on their future global distribution changes, whereas a single study focused on its effects on Ae. aegypti densities regionally. Objectives: A process-based approach was used to model densities of Ae. aegypti and Ae. albopictus and their potential evolution with climate change using a panel of nine CMIP6 climate models and climate scenarios ranging from strong to low mitigation measures at the Southeast Asian scale and for the next 80 y. Methods: The process-based model described, through a system of ordinary differential equations, the variations of mosquito densities in 10 compartments, corresponding to 10 different stages of mosquito life cycle, in response to temperature and precipitation variations. Local field data were used to validate model outputs. Results: We show that both species densities will globally increase due to future temperature increases. In Southeast Asia by the end of the century, Ae. aegypti densities are expected to increase from 25% with climate mitigation measures to 46% without; Ae. albopictus densities are expected to increase from 13%–21%, respectively. However, we find spatially contrasted responses at the seasonal scales with a significant decrease in Ae. albopictus densities in lowlands during summer in the future. Discussion: These results contrast with previous results, which brings new insight on the future impacts of climate change on Aedes densities. Major sources of uncertainties, such as mosquito model parametrization and climate model uncertainties, were addressed to explore the limits of such modeling. https://doi.org/10.1289/EHP11068
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- 2022
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12. Identification of a novel neuropathogenic theiler’s murine encephalomyelitis virus
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Buckwalter , Matthew R., Nga , Phan Thi, Gouilh , Meriadeg Ar., Fiette , Laurence, Bureau , Jean-François, Laird , Melissa E., Buchrieser , Julian, Ozden , Simona, Cheval , Justine, Eloit , Marc, Manuguerra , Jean-Claude, Gessain , Antoine, Brey , Paul T., Fontanet , Arnaud, Albert , Matthew L., Immunobiologie des Cellules Dendritiques, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), National Institute of Hygiene and Epidemiology ( NIHE ), Cellule d'Intervention Biologique d'Urgence ( CIBU ), Institut Pasteur [Paris], Histopathologie humaine et Modèles animaux, Département de Virologie, Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Génotypage des Pathogènes et Santé Publique (Plate-forme) ( PF8 ), Virologie UMR1161 ( VIRO ), Institut National de la Recherche Agronomique ( INRA ) -Ecole Nationale Vétérinaire d'Alfort-ANSES, Institut Pasteur du Laos, Réseau International des Instituts Pasteur ( RIIP ) -Institut Pasteur du Laos, Epidémiologie des Maladies Emergentes, Conservatoire National des Arts et Métiers [CNAM] ( CNAM ) -Institut Pasteur [Paris]-Pasteur-Cnam risques infectieux et émergents ( PACRI ), Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] : EA4628-Conservatoire National des Arts et Métiers [CNAM] : EA4628, and Université Paris Descartes - Paris 5 ( UPD5 )
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[ SDV ] Life Sciences [q-bio] ,health care facilities, manpower, and services ,Neuropathogenic ,education ,Theiler’s ,Murine ,health care economics and organizations - Abstract
International audience; absent
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- 2011
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13. A genomic amplification affecting a carboxylesterase gene cluster confers organophosphate resistance in the mosquito Aedes aegypti: From genomic characterization to high‐throughput field detection
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Jean-Marc Bonneville, Frederic Laporte, Ian W. Sutherland, Benedicte Fustec, Jeffrey C. Hertz, Tristan Cumer, Sébastien Marcombe, Julien Renaud, Thierry Gaude, Jean-Philippe David, Sébastien Boyer, Camille Noûs, Victor Arnaud, Chloé Haberkorn, Julien Cattel, Laboratoire d'Ecologie Alpine (LECA ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Naval Air Station Jacksonville (NAS Jacksonville), United States Navy - U.S. Navy (USA), Naval Medical Research Unit Two [Singapore] (NAMRU-2), Naval Medical Research, Khon Kaen University [Thailand] (KKU), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur du Laos, This work was conducted in the framework of the U.S. Naval Medical Research Unit TWO projects BIO‐LAO‐2 (work unit number D1425) and ARBOVEC‐PLUS (work unit number D1428), in support and funded by the Department of Defense Global Emerging Infections Surveillance Program and Military Infectious Disease Research Program. I (IWS and JCH) am a military Service member. This work was prepared as part of my official duties. Title 17, U.S.C., Section 105 provides that copyright protection under this title is not available for any work of the U.S. Government. Title 17, U.S.C., Section 101 defines a U.S. Government work as a work prepared by a military Service member or employee of the U.S. Government as part of that person's official duties. This publication was also supported by the project, Research Infrastructures for the control of vector‐borne diseases (Infravec2), which has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 731060. Dr. Julien Cattel was supported by funding from the European Union's Horizon 2020 Research and Innovation Programme under ZIKAlliance Grant Agreement no. 734548. The field study in Cambodia was supported by ECOMORE 2 project, coordinated by Institut Pasteur and financially supported by AFD (Agence Française pour le Développement)., European Project: 731060,INFRAVEC2(2017), European Project: 734548,ZIKAlliance(2016), United States Navy Entomology, Laboratoire Cogitamus, Laboratoire d'Ecologie Alpine (LECA), and Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
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0106 biological sciences ,0301 basic medicine ,Evolution ,[SDV]Life Sciences [q-bio] ,030231 tropical medicine ,Population ,Locus (genetics) ,mosquito ,Aedes aegypti ,Biology ,010603 evolutionary biology ,01 natural sciences ,03 medical and health sciences ,0302 clinical medicine ,diagnostic assay ,Gene cluster ,Gene duplication ,Genetics ,QH359-425 ,Multiplex ,Copy-number variation ,education ,Gene ,ComputingMilieux_MISCELLANEOUS ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,Whole genome sequencing ,0303 health sciences ,education.field_of_study ,Haplotype ,Original Articles ,insecticide resistance ,carboxylesterase ,biology.organism_classification ,030104 developmental biology ,[SDE]Environmental Sciences ,genomic amplification ,Original Article ,General Agricultural and Biological Sciences - Abstract
By altering gene expression and creating paralogs, genomic amplifications represent a key component of short-term adaptive processes. In insects, the use of insecticides can select gene amplifications causing an increased expression of detoxification enzymes, supporting the usefulness of these DNA markers for monitoring the dynamics of resistance alleles in the field. In this context, the present study aims to characterise a genomic amplification event associated with resistance to organophosphate insecticides in the mosquito Aedes aegypti and to develop a molecular assay to monitor the associated resistance alleles in the field. An experimental evolution experiment using a composite population from Laos supported the association between the over-transcription of multiple contiguous carboxylesterase genes on chromosome 2 and resistance to multiple organophosphate insecticides. Combining whole genome sequencing and qPCR on specific genes confirmed the presence of a ~100 Kb amplification spanning at least five carboxylesterase genes at this locus with the co-existence of multiple structural duplication haplotypes. Field data confirmed their circulation in South-East Asia and revealed high copy number polymorphism among and within populations suggesting a trade-off between this resistance mechanism and associated fitness costs. A dual-colour multiplex TaqMan assay allowing the rapid detection and copy number quantification of this amplification event in Ae. aegypti was developed and validated on field populations. The routine use of this novel assay will improve the tracking of resistance alleles in this major arbovirus vector.
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- 2021
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14. MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species
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Antsa Rakotonirina, Morgane Pol, Fara Nantenaina Raharimalala, Valentine Ballan, Malia Kainiu, Sébastien Boyer, Sosiasi Kilama, Sébastien Marcombe, Sylvie Russet, Emilie Barsac, Rama Vineshwaran, Malia Kaleméli Selemago, Vincent Jessop, Geneviève Robic, Romain Girod, Paul T. Brey, Julien Colot, Myrielle Dupont-Rouzeyrol, Vincent Richard, Nicolas Pocquet, Institut Pasteur de Nouvelle-Calédonie, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Madagascar, Institut Pasteur du Cambodge, Écologie et Émergence des Pathogènes Transmis par les Arthropodes / Ecology and Emergence of Arthropod-borne Pathogens, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut Pasteur du Laos, Ministry of Health [Fiji], Agence de Santé des îles Wallis & Futuna, Direction Internationale de l'Institut Pasteur, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), and The project leading to this publication received funding from the French Fund for Economic, Social, Cultural and Scientific Cooperation in the Pacific ('Pacific Fund') and the and the Institut Pasteur of New Caledonia. AR obtained a scholarship from the Institut Pasteur International Network (Bourse Calmette & Yersin).
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[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Dengue ,Multidisciplinary ,Internationality ,Aedes ,Zika Virus Infection ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Commerce ,Humans ,Animals ,Mosquito Vectors ,Zika Virus ,Arboviruses - Abstract
Dengue, Zika and chikungunya viruses cause significant human public health burdens in the world. These arboviruses are transmitted by vector mosquito species notably Aedes aegypti and Aedes albopictus. In the Pacific region, more vector species of arboviruses belonging to the Scutellaris Group are present. Due to the expansion of human travel and international trade, the threat of their dispersal in other world regions is on the rise. Strengthening of entomological surveillance ensuring rapid detection of introduced vector species is therefore required in order to avoid their establishment and the risk of arbovirus outbreaks. This surveillance relies on accurate species identification. The aim of this study was to assess the use of the Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) as a tool for an international identification and surveillance of these mosquito vectors of arboviruses. Field-mosquitoes belonging to 8 species (Ae. aegypti, Ae. albopictus, Aedes polynesiensis, Aedes scutellaris, Aedes pseudoscutellaris, Aedes malayensis, Aedes futunae and Culex quinquefasciatus) from 6 countries in the Pacific, Asian and Madagascar, were included in this study. Analysis provided evidence that a MALDI-TOF database created using mosquitoes from the Pacific region allowed suitable identification of mosquito species from the other regions. This technic was as efficient as the DNA sequencing method in identifying mosquito species. Indeed, with the exception of two Ae. pseudoscutellaris, an exact species identification was obtained for all individual mosquitoes. These findings highlight that the MALDI-TOF MS is a promising tool that could be used for a global comprehensive arbovirus vector surveillance.
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- 2022
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15. Bat coronaviruses related to SARS-CoV-2 and infectious for human cells
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Sarah Temmam, Khamsing Vongphayloth, Eduard Baquero, Sandie Munier, Massimiliano Bonomi, Béatrice Regnault, Bounsavane Douangboubpha, Yasaman Karami, Delphine Chrétien, Daosavanh Sanamxay, Vilakhan Xayaphet, Phetphoumin Paphaphanh, Vincent Lacoste, Somphavanh Somlor, Khaithong Lakeomany, Nothasin Phommavanh, Philippe Pérot, Océane Dehan, Faustine Amara, Flora Donati, Thomas Bigot, Michael Nilges, Félix A. Rey, Sylvie van der Werf, Paul T. Brey, Marc Eloit, Découverte de pathogènes – Pathogen discovery, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Centre Collaborateur de l'OIE de Détection et identification chez l’homme des pathogènes animaux émergents et développement d’outils pour leur diagnostic / Collaborating Center for the Detection and identification in humans of emerging animal pathogens and development of tools for their diagnoses (CCOIE-OIECC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé Animale / World Organisation Animal Health [Paris] (OIE)-Université Paris Cité (UPCité), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Virologie Structurale - Structural Virology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses (GMV-ARN (UMR_3569 / U-Pasteur_2)), Bioinformatique structurale - Structural Bioinformatics, National University of Laos, Centre National de Référence des virus des infections respiratoires (dont la grippe) - National Reference Center Virus Influenzae [Paris] (CNR - laboratoire coordonnateur), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, École nationale vétérinaire - Alfort (ENVA), NGS was performed with the help of the Biomics Platform, Center for Technological Resources and Research, Institut Pasteur, Paris, France, supported by France Génomique (ANR-10-INBS-09-09), Inclusive Blockchain Insurance using Space Assets and the Illumina COVID-19 Projects’ offer. The work was granted access to the High Performance Computing resources of the Institute for Development and Resources in Intensive Scientific Computing under the allocation 2020-101592 made by Grand Équipement National de Calcul Intensif. We thank the Ministry of Health and the Ministry of Natural Resources and Environments, Lao People’s Democratic Republic, for their authorization of the field work and the Faculty of Environmental Science for its authorization of the field research collaboration. The work was supported by an Institut Pasteur 'Covid Taskforce' and in part by the H2020 project 101003589 (RECOVER) and Labex IBEID (ANR-10-LABX62-IBEID) grants. Field and laboratory work at IPL was also supported by a UK embassy grant (grant no. INT 2021/LOV C19 02) and a Luxembourg Development special grant (grant no. LAO/030·202324)., We thank S. Mohamed Ali, N. Da Rocha, A. Brisebarre, T. Xaybounsou and S. Chonephetsarath for their help at the bench, A. Haouz and the staff of the protein crystallogenesis facility at Institut Pasteur for help with crystallization trials, P. England from the Molecular Biophysics facility at Institut Pasteur for his support and access to the BLI equipment, P. Legrand from the PX1 beamline at Synchrotron SOLEIL for data collection support, and P. Guardado-Calvo for discussion and support with BLI experiments., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), and European Project: 101003589, H2020-SC1-PHE-CORONAVIRUS-2020,RECOVER(2020)
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Asia ,viruses ,MESH: Spike Glycoprotein, Coronavirus ,MESH: Angiotensin-Converting Enzyme 2 ,Chiroptera ,Animals ,Humans ,MESH: Protein Binding ,MESH: COVID-19 ,MESH: Animals ,MESH: SARS-CoV-2 ,skin and connective tissue diseases ,Taxonomy ,Disease Reservoirs ,MESH: Disease Reservoirs ,Multidisciplinary ,MESH: Humans ,SARS-CoV-2 ,MESH: Asia ,[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] ,fungi ,COVID-19 ,MESH: Chiroptera ,Biodiversity ,body regions ,Caves ,Spike Glycoprotein, Coronavirus ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,MESH: Caves ,Angiotensin-Converting Enzyme 2 ,Protein Binding - Abstract
International audience; The animal reservoir of SARS-CoV-2 is unknown despite reports of SARS-CoV-2-related viruses in Asian Rhinolophus bats1-4, including the closest virus from R. affinis, RaTG13 (refs. 5,6), and pangolins7-9. SARS-CoV-2 has a mosaic genome, to which different progenitors contribute. The spike sequence determines the binding affinity and accessibility of its receptor-binding domain to the cellular angiotensin-converting enzyme 2 (ACE2) receptor and is responsible for host range10-12. SARS-CoV-2 progenitor bat viruses genetically close to SARS-CoV-2 and able to enter human cells through a human ACE2 (hACE2) pathway have not yet been identified, although they would be key in understanding the origin of the epidemic. Here we show that such viruses circulate in cave bats living in the limestone karstic terrain in northern Laos, in the Indochinese peninsula. We found that the receptor-binding domains of these viruses differ from that of SARS-CoV-2 by only one or two residues at the interface with ACE2, bind more efficiently to the hACE2 protein than that of the SARS-CoV-2 strain isolated in Wuhan from early human cases, and mediate hACE2-dependent entry and replication in human cells, which is inhibited by antibodies that neutralize SARS-CoV-2. None of these bat viruses contains a furin cleavage site in the spike protein. Our findings therefore indicate that bat-borne SARS-CoV-2-like viruses that are potentially infectious for humans circulate in Rhinolophus spp. in the Indochinese peninsula.
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- 2022
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16. De nouveaux coronavirus de chauve-souris similaires à SARS-CoV-2 éclairent l'origine de la pandémie de COVID-19
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Temmam, Sarah, Vongphayloth, Khamsing, Baquero Salazar, Eduard, Munier, Sandie, Bonomi, Massimiliano, Regnault, Béatrice, Douangboubpha, Bounsavane, Karami, Yasaman, Chrétien, Delphine, Sanamxay, Daosavanh, Xayaphet, Vilakhan, Paphaphanh, Phetphoumin, Lacoste, Vincent, Somlor, Somphavanh, Lakeomany, Khaithong, Phommavanh, Nothasin, Pérot, Philippe, Dehan, Océane, Amara, Faustine, Donati, Flora, Bigot, Thomas, Nilges, Michael, Rey, Félix, van Der Werf, Sylvie, Brey, Paul, Eloit, Marc, Découverte de pathogènes – Pathogen discovery, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Centre Collaborateur de l'OIE de Détection et identification chez l’homme des pathogènes animaux émergents et développement d’outils pour leur diagnostic / Collaborating Center for the Detection and identification in humans of emerging animal pathogens and development of tools for their diagnoses (CCOIE-OIECC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé Animale / World Organisation Animal Health [Paris] (OIE)-Université Paris Cité (UPCité), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Virologie Structurale - Structural Virology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses (GMV-ARN (UMR_3569 / U-Pasteur_2)), Bioinformatique structurale - Structural Bioinformatics, National University of Laos, Centre National de Référence des virus des infections respiratoires (dont la grippe) - National Reference Center Virus Influenzae [Paris] (CNR - laboratoire coordonnateur), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, and TEMMAM, Sarah
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[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDE.BE] Environmental Sciences/Biodiversity and Ecology ,pandémie ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,coronavirus ,chauve-souris ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
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- 2022
17. Coronaviruses with a SARS-CoV-2-like receptor-binding domain allowing ACE2-mediated entry into human cells isolated from bats of Indochinese peninsula
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Khamsing Vongphayloth, Delphine Chrétien, Yasaman Karami, Vilakhan Xayaphet, Sarah Temmam, Félix A. Rey, Max Bonomi, Somphavanh Somlor, Khaithong Lakeomany, Bounsavane Douangboubpha, Marc Eloit, Phetphoumin Paphaphanh, Nothasin Phommavanh, Michael Nilges, Flora Donati, Paul T. Brey, Daosavanh Sanamxay, Vincent Lacoste, Thomas Bigot, Sandie Munier, Béatrice Regnault, Sylvie van der Werf, Philippe Pérot, Eduard Baquero Salazar, Institut Pasteur [Paris] (IP), National University of Laos, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), The work was funded by an Institut Pasteur 'Covid Taskforce' and in part by the H2020 project 101003589 (RECOVER) grants. Field and laboratory work at IP-Laos was also funded by a UK embassy grant (Grant No. INT 2021/LOV C19 02) and Luxembourg Development special grant (Grant No. LAO/030·202324)., The authors want to thank Souand Mohamed Ali, Nicolas Da Rocha, Thonglakhone Xaybounsou, and Somsanith Chonephetsarath for their help at the bench. Next-generation sequencing was performed with the help of Biomics Platform, C2RT, Institut Pasteur, Paris, France, supported by France Génomique (ANR-10-INBS-09-09), IBISA, and the Illumina COVID-19 Projects’ offer. The work was granted access to the HPC resources of IDRIS under the allocation 2020-101592 made by GENCI. We thank the Ministry of Health and the Ministry of Natural Resources and Environments, Lao PDR, for their authorization of the field work and the Faculty of Environmental Science for its authorization of the field research collaboration., ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), European Project: 101003589, H2020-SC1-PHE-CORONAVIRUS-2020,RECOVER(2020), and Institut Pasteur [Paris]
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0303 health sciences ,Indochinese peninsula ,030306 microbiology ,Coronaviruses ,SARS-CoV-2 ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,viruses ,[SDV]Life Sciences [q-bio] ,fungi ,virus diseases ,Biology ,Virology ,cave bats ,3. Good health ,Domain (software engineering) ,body regions ,03 medical and health sciences ,spike sequence ,receptor-binding domain ,skin and connective tissue diseases ,angiotensin-converting enzyme 2 pathway ,030304 developmental biology - Abstract
The animal reservoir of SARS-CoV-2 is unknown despite reports of various SARS-CoV-2-related viruses in Asian Rhinolophus bats, including the closest virus from R. affinis, RaTG13. Several studies have suggested the involvement of pangolin coronaviruses in SARS-CoV-2 emergence. SARS-CoV-2 presents a mosaic genome, to which different progenitors contribute. The spike sequence determines the binding affinity and accessibility of its receptor-binding domain (RBD) to the cellular angiotensin-converting enzyme 2 (ACE2) receptor and is responsible for host range. SARS-CoV-2 progenitor bat viruses genetically close to SARS-CoV-2 and able to enter human cells through a human ACE2 pathway have not yet been identified, though they would be key in understanding the origin of the epidemics. Here we show that such viruses indeed circulate in cave bats living in the limestone karstic terrain in North Laos, within the Indochinese peninsula. We found that the RBDs of these viruses differ from that of SARS-CoV-2 by only one or two residues, bind as efficiently to the hACE2 protein as the SARS-CoV-2 Wuhan strain isolated in early human cases, and mediate hACE2-dependent entry into human cells, which is inhibited by antibodies neutralizing SARS-CoV-2. None of these bat viruses harbors a furin cleavage site in the spike. Our findings therefore indicate that bat-borne SARS-CoV-2-like viruses potentially infectious for humans circulate in Rhinolophus spp. in the Indochinese peninsula.
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- 2021
18. The influence of habitat on viral diversity in neotropical rodent hosts
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Anne Lavergne, Damien Donato, Alain Franc, Sourakhata Tirera, Benoit de Thoisy, Christiane Bouchier, Vincent Lacoste, Laboratoire des Interactions Virus-Hôtes [Cayenne, Guyane Française], Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institut Pasteur [Paris], Département de Virologie - Department of Virology, Arbovirus & Emerging Viral Diseases [Vientiane] (A&EVD), Institut Pasteur du Laos, Biodiversité, Gènes & Communautés (BioGeCo), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), from patterns to models in computational biodiversity and biotechnology (PLEIADE), Laboratoire Bordelais de Recherche en Informatique (LaBRI), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Biodiversité, Gènes & Communautés (BioGeCo), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), S. Tirera was funded by the RESERVOIRS program, supported by European funds (PO FSE 2014-2020), an 'Investissement d’Avenir' grant managed by the Agence Nationale de la Recherche (CEBA, ref. ANR-10-LABX-25-01), a European Commission 'REGPOT-CT-2011-285837-STRonGer' grant within the FP7, and the Institut Pasteur de la Guyane. This study was conducted within the 'BioViRo' program, supported by an 'Investissement d’Avenir' grant managed by the Agence Nationale de la Recherche (CEBA, reference ANR-10-LABX-25-01). Field work conducted by BdT was funded by the ViRUSES program, supported by European funds (FEDER) and assistance from Région Guyane and Direction Régionale pour la Recherche et la Technologie, the ZNIEFF Guyane (DEAL Guyane) program, the GUYAMAZON II program, and Réseau des Observatoires Hommes-Milieux (OHM-Oyapok APR 2013, François Catzeflis). High-throughput sequencing was performed on the Biomics Platform, C2RT, Institut Pasteur, Paris, France, supported by France Génomique (ANR-10-INBS-09-09) and IBISA., ANR-10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010), ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), European Project: 285837,EC:FP7:REGPOT,FP7-REGPOT-2011-1,STRONGER(2011), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), and Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Inria Bordeaux - Sud-Ouest
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Viral ecology ,Ecology (disciplines) ,viruses ,Rodentia ,Forests ,Biology ,Microbiology ,Rodents ,Article ,Viral phylogenies ,03 medical and health sciences ,Amazonia ,Abundance (ecology) ,Zoonoses ,Virology ,Animals ,Human virome ,Alpha diversity ,Ecosystem ,Phylogeny ,030304 developmental biology ,0303 health sciences ,Ecology ,030306 microbiology ,Host (biology) ,Virome ,Genetic Variation ,15. Life on land ,QR1-502 ,3. Good health ,Infectious Diseases ,Habitat ,Metagenomics ,Viruses ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDE]Environmental Sciences ,Metagenome ,Species richness - Abstract
Rodents are important reservoirs of numerous viruses, some of which have significant impacts on public health. Ecosystem disturbances and decreased host species richness have been associated with the emergence of zoonotic diseases. In this study, we aimed at (a) characterizing the viral diversity in seven neotropical rodent species living in four types of habitats and (b) exploring how the extent of environmental disturbance influences this diversity. Through a metagenomic approach, we identified 77,767 viral sequences from spleen, kidney, and serum samples. These viral sequences were attributed to 27 viral families known to infect vertebrates, invertebrates, plants, and amoeba. Viral diversities were greater in pristine habitats compared with disturbed ones, and lowest in peri-urban areas. High viral richness was observed in savannah areas. Differences in these diversities were explained by rare viruses that were generally more frequent in pristine forest and savannah habitats. Moreover, changes in the ecology and behavior of rodent hosts, in a given habitat, such as modifications to the diet in disturbed vs. pristine forests, are major determinants of viral composition. Lastly, the phylogenetic relationships of four vertebrate-related viral families (Polyomaviridae, Flaviviridae, Togaviridae, and Phenuiviridae) highlighted the wide diversity of these viral families, and in some cases, a potential risk of transmission to humans. All these findings provide significant insights into the diversity of rodent viruses in Amazonia, and emphasize that habitats and the host’s dietary ecology may drive viral diversity. Linking viral richness and abundance to the ecology of their hosts and their responses to habitat disturbance could be the starting point for a better understanding of viral emergence and for future management of ecosystems.
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- 2021
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19. Using phylogenetic surveillance and epidemiological data to understand the HIV-1 transmission dynamics in French Guiana
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Mathieu Nacher, Ighor Arantes, Edith Darcissac, Gonzalo Bello, Vincent Lacoste, Instituto Oswaldo Cruz / Oswaldo Cruz Institute [Rio de Janeiro] (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur du Laos, Centre d'Investigation Clinique Antilles-Guyane (CIC - Antilles Guyane), Université des Antilles et de la Guyane (UAG)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -CHU de Fort de France-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Université de Guyane (UG), and IA was funded by a fellowship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES-Brazil). G.B. is funded by fellowships from the Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq (Grant number 302317/2017–1) and the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro – FAPERJ (Grant number E-26/202.896/2018). This research was supported by grant 14078 from the ANRS.
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0301 basic medicine ,medicine.medical_specialty ,Maximum likelihood ,Immunology ,HIV Infections ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Phylogenetics ,law ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Epidemiology ,medicine ,Humans ,Immunology and Allergy ,Statistical analysis ,030212 general & internal medicine ,Clade ,Phylogeny ,Phylogenetic tree ,pandemic ,[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] ,Bayes Theorem ,South America ,phylodynamics ,nonpandemic ,French Guiana ,030104 developmental biology ,Infectious Diseases ,Transmission (mechanics) ,Hiv 1 transmission ,Geography ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,HIV-1 ,epidemiology ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,subtype B ,Demography - Abstract
International audience; Objective: The aim of this study was to understand the transmission dynamics of the HIV-1 subtype B epidemic in French Guiana and the factors that shaped the expansion of major phylogenetic transmission clusters.Design: HIV-1 subtype B pol sequences with associated epidemiological data obtained from 703 treatment-naive patients living in French Guiana between 2006 and 2012, which correspond to 91% of all HIV cases diagnosed in that period, were employed in this study.Methods: Maximum likelihood and Bayesian methods were used to construct phylogenetic trees, identify transmission clusters and estimate intervals between successive infections. Statistical analysis was performed to evaluate epidemiological characteristics associated with cluster membership.Results: HIV-1 subtype B pol sequences from French Guiana were distributed in 10 large/medium transmission clades (LMTC, n > 10, 55%), 19 small transmission clades (STC, n = 3–8, 10%), 36 dyads (10%) or were nonclustered (25%). The rate of clustering did not differ by sex or clinical stage, but sex workers, crack-cocaine users, young individuals (15–20 years) and nationals or migrants from neighbouring South American countries were more likely to cluster within LMTC than individuals from other groups. We estimated that 53–63% of immigrants were infected after the arrival in French Guiana and that 50% of HIV transmissions within LMTC occurred during the first 2 years after infection.Conclusion: These findings reinforce the notion that high-risk sexual behaviours among young individuals and migrants (postmigration) combined with late HIV diagnosis are key drivers of onward dissemination of major HIV transmission clusters in French Guiana.
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- 2021
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20. Evaluation of Zika rapid tests as aids for clinical diagnosis and epidemic preparedness
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Debi Boeras, Cheikh Tidiane Diagne, Jose L. Pelegrino, Marc Grandadam, Veasna Duong, Philippe Dussart, Paul Brey, Didye Ruiz, Marisa Adati, Annelies Wilder-Smith, Andrew K. Falconar, Claudia M. Romero, Maria Guzman, Nagwa Hasanin, Amadou Sall, Rosanna W. Peeling, Global Health Impact Group, Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP), Pedro Kouri Institute of Tropical Medicine, Institut Pasteur du Laos, Institut Pasteur du Cambodge, Institut Pasteur de Madagascar, Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), Umeå University, London School of Hygiene and Tropical Medicine (LSHTM), Universidad del Norte, Barranquilla, UNICEF Headquarters, This work was supported by the USAID grant GHA-G-00-07-00007 and ZikaPLAN (European Union's Horizon 2020 Research and Innovation Programme under Grant Agreement No. 734584)., and European Project: 734584,ZikaPLAN
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Infectious Medicine ,[SDV]Life Sciences [q-bio] ,Infektionsmedicin ,Public Health, Global Health, Social Medicine and Epidemiology ,General Medicine ,Advance purchase commitment ,Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi ,Epidemic preparedness ,Zika ,Clinical medicine ,parasitic diseases ,Biobanking network ,Evaluation ,Diagnostics - Abstract
Background: Development and evaluation of diagnostics for diseases of epidemic potential are often funded during epidemics, but not afterwards, leaving countries unprepared for the next epidemic. United Nations Children's Emergency Fund (UNICEF) partnered with the United States Agency for International Development (USAID) to address this important gap by investing in an advance purchase commitment (APC) mechanism to accelerate the development and evaluation of Zika rapid diagnostic tests (RDTs) for case detection and surveillance. This paper describes the performance evaluation of five Zika RDTs eligible for procurement. Methods: A network of European Union-funded ZikaPLAN sites in Africa, Asia, Latin America with access to relevant serum specimens were selected to evaluate RDTs developed for the UNICEF APC mechanism. A standardised protocol and evaluation panels were developed and a call for specimens for the evaluation panels issued to different sites. Each site contributed specimens to the evaluation from their biobank. Data were collated, analysed and presented to the UNICEF Procurement Review Group for review. Findings: Three RDTs met the criteria for UNICEF procurement of sensitivity and specificity of 85% against a refence standard. The sensitivity/specificity of the ChemBio anti-Zika Virus (ZIKV) immunoglobulin M (IgM) test was 86.4 %/86.7% and the ChemBio ZCD system for anti-ZIKV IgM was 79.0%/97.1%, anti-dengue virus (DENV) IgM 90.0%/89.2%, anti-Chikungunya virus (CHIKV) IgM 90.6%/97.2%. The sensitivity/specificity of the SD Biosensor anti-ZIKV IgM was 96.8 %/90.8%, anti-DENV IgM 71.8%/83.5%, the DENV nonstructural protein 1 (NS1) glycoprotein 90.0%/90.2%, anti- yellow fever virus (YFV) IgM 84.6%/92.4%, anti-CHIKV IgM 86.3%/97.5%. Interpretation: Three RDTs fulfilled the performance thresholds set by WHO and were eligible for UNICEF procurement. These tests will improve the diagnosis of ZIKV and other arboviral infections as well as providing countries with better tools for surveillance and response to future epidemics. Funding: This work was supported by the USAID grant GHA-G-00-07-00007 and ZikaPLAN (European Union's Horizon 2020 Research and Innovation Programme under Grant Agreement No. 734584).
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- 2022
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21. Using Background Sequencing Data to Anticipate DENV-1 Circulation in the Lao PDR
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Phaithong Bounmany, Vincent Lacoste, Marc Grandadam, Charlotte Balière, Valérie Caro, Sitsana Keosenhom, Thonglakhone Xaybounsou, Paul T. Brey, Kitphithak Fangkham, Souksakhone Viengphouthong, Elodie Calvez, Somphavanh Somlor, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Environnement et Risques infectieux - Environment and Infectious Risks (ERI), Institut Pasteur [Paris]-Université Paris Cité (UPC), Institut Pasteur [Paris] (IP), and This work was funded by the Agence Française de Développement grant N° CZZ 2146 01 (Ecomore2 project) and by UNITEDengue and the Global Partnership Program, Canada (ASEAN-GPP Grant Phase 3—Laboratory Capacity Development for diagnostics of Emerging Dangerous Pathogens).
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Microbiology (medical) ,Serotype ,medicine.medical_specialty ,QH301-705.5 ,viruses ,Population ,Dengue virus ,medicine.disease_cause ,phylogeny ,[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy ,Microbiology ,Article ,Dengue fever ,Lao PDR ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Virology ,Environmental health ,Epidemiology ,medicine ,Circulation (currency) ,Biology (General) ,education ,education.field_of_study ,[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases ,Public health ,DENV-1 ,Outbreak ,virus diseases ,biochemical phenomena, metabolism, and nutrition ,medicine.disease ,dengue ,Geography ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,epidemiology ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
International audience; Since its first detection in 1979, dengue fever has been considered a major public health issue in the Lao People’s Democratic Republic (PDR). Dengue virus (DENV) serotype 1 was the cause of an epidemic in 2010–2011. Between 2012 and 2020, major outbreaks due successively to DENV-3, DENV-4 and recently DENV-2 have been recorded. However, DENV-1 still co-circulated in the country over this period. Here, we summarize epidemiological and molecular data of DENV-1 between 2016 and 2020 in the Lao PDR. Our data highlight the continuous circulation of DENV-1 in the country at levels ranging from 16% to 22% among serotyping tests. In addition, the phylogenetic analysis has revealed the circulation of DENV-1 genotype I at least since 2008 with a co-circulation of different clusters. Sequence data support independent DENV-1 introductions in the Lao PDR correlated with an active circulation of this serotype at the regional level in Southeast Asia. The maintenance of DENV-1 circulation over the last ten years supports a low level of immunity against this serotype within the Lao population. Thereby, the risk of a DENV-1 epidemic cannot be ruled out in the future, and this emphasizes the importance of maintaining an integrated surveillance approach to prevent major outbreaks
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- 2021
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22. New insights into malaria vector bionomics in Lao PDR: a nationwide entomology survey
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Nothasin Phommavan, Vincent Corbel, Simone Nambanya, Julie Bobichon, Santi Maithaviphet, Paul T. Brey, Sébastien Marcombe, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Ministry of Health [Laos], Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), and This work was carried out within the framework of the MALVEC research project funded by the 5% initiative from the French Ministry of Foreign Affairs and International Development, contract12INI212.
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Veterinary medicine ,lcsh:Arctic medicine. Tropical medicine ,Primary and secondary vectors ,lcsh:RC955-962 ,030231 tropical medicine ,Plasmodium vivax ,Plasmodium falciparum ,Population Dynamics ,Anopheles maculatus ,Anopheles aconitus ,Context (language use) ,Mosquito Vectors ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,0302 clinical medicine ,Anopheles dirus ,Anopheles ,parasitic diseases ,medicine ,Animals ,Biting preferences ,lcsh:RC109-216 ,030212 general & internal medicine ,Anopheles minimus ,Plasmodium infection ,Life History Traits ,biology ,Research ,Feeding Behavior ,biology.organism_classification ,medicine.disease ,Malaria ,3. Good health ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Infectious Diseases ,Laos ,Vector (epidemiology) ,Parasitology ,Female ,Animal Distribution - Abstract
Background In Laos, the malaria burden remains high despite a significant reduction of cases during the last decade. In the context of the disease elimination by 2030, a nationwide entomological survey was conducted to better understand the distribution, abundance and behaviour of major malaria vectors (Anopheles spp.) in the country. Methods Mosquito collections were implemented in ten villages from ten provinces during the rainy and dry seasons of 2014 and 2015 by using human landing catch (HLC) and cow bait collection (CBC) methods. After morphological identification in the field, molecular identification of the sibling species of Anopheles mosquitoes from the Funestus, Leucosphyrus, and Maculatus groups were determined using PCR specific alleles. A screening of Plasmodium falciparum and Plasmodium vivax infections in the vectors was carried out by quantitative PCR assays. Results A total of 14,146 adult mosquitoes representing 25 different Anopheles species were collected and morphologically identified. Molecular identification revealed the presence of 12 sibling species within the main primary vector groups, including Anopheles maculatus, Anopheles rampae, Anopheles sawadwongporni, Anopheles pseudowillmori, Anopheles dravidicus, Anopheles minimus, Anopheles aconitus, Anopheles pampanai, Anopheles harrisoni, Anopheles dirus, Anopheles baimaii, Anopheles nemophilous. Anopheles maculatus and An. minimus were predominant during both the dry and rainy seasons, but showed highly zoophilic preferences (Zoophilic index of 98% and 95%, respectively). Overall, 22% of the total malaria vectors were collected between 10:00 PM and 5:00 AM indoors when people are sleeping. Twenty-seven percent of primary and secondary vectors were collected outdoors before 10:00 PM or after 5:00 AM, times when people are usually awake and outdoors. Only two specimens were positive for P. falciparum, one An. aconitus from Phongsaly and one An. minimus from Vientiane Province Conclusions The results indicate that people living in rural areas in Laos are constantly exposed to malaria vectors throughout the year and specifically outdoors. The use of LLINs/IRS remains important but innovative tools and new strategies are needed to address locally, the early and outdoor malaria transmission. Lack of expertise in general entomological methods may further exacerbate the situation.
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- 2020
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23. Malaria and Dengue Mosquito Vectors from Lao PDR Show a Lack of the rdl Mutant Allele Responsible for Cyclodiene Insecticide Resistance
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Susannah Lechmere, Omobolanle H Abdullateef, Phoutmany Thammavong, Natalie M Portwood, Safina Khadam, Chloé Boer, Somsanith Chonephetsarath, Rosie Longstreeth, Paul T. Brey, Anna E Jacob, Nursu C Kuyucu, Wlaa Ali, Phonesavanh Luangamath, Jordan Forward, Andrew K. Jones, Santi Maithaviphet, Sébastien Marcombe, Somphat Nilaxay, Zuhal Rahmani, Simone Nambanya, Hayato Kakinuma, Natasha Brown, Joseph Hawkins, Penelope J Saverton, Madeleine Smee, Khaitong Lakeomany, Gabriela Stieger, Nothasin Phommavanh, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Oxford Brookes University, Ministry of Health [Laos], and The mosquito collections, rearing and identification work was carried out within the framework of the MALVEC research project funded by the 5% initiative from the French Ministry of Foreign Affairs and International Development. For extraction and amplification of DNA from mosquitoes, funding was provided by Oxford Brookes University.
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Insecticides ,MESH: Dengue ,medicine.disease_cause ,Dengue fever ,Dengue ,chemistry.chemical_compound ,0302 clinical medicine ,Aedes ,MESH: Insect Proteins ,MESH: Animals ,0303 health sciences ,Mutation ,biology ,Anopheles ,dieldrin ,insecticide resistance ,MESH: Aedes ,3. Good health ,Infectious Diseases ,Laos ,Insect Proteins ,MESH: Mosquito Vectors ,Aedes albopictus ,MESH: Mutation ,030231 tropical medicine ,MESH: Malaria ,mosquito ,Aedes aegypti ,Mosquito Vectors ,MESH: Anopheles ,03 medical and health sciences ,Dieldrin ,GABA receptor ,parasitic diseases ,medicine ,Animals ,MESH: Insecticide Resistance ,Alleles ,030304 developmental biology ,General Veterinary ,MESH: Alleles ,fungi ,medicine.disease ,biology.organism_classification ,Virology ,Malaria ,MESH: Insecticides ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,chemistry ,MESH: Laos ,Insect Science ,MESH: Dieldrin ,Parasitology - Abstract
The gamma-aminobutyric acid (GABA) receptor, RDL, plays important roles in neuronal signaling and is the target of highly effective insecticides. A mutation in RDL, commonly A296S, underlies resistance to several insecticides such as cyclodienes. Even though the use of cyclodienes has been banned, the occurrence of mutations substituting A296 is notably high in mosquitoes from several countries. Here, we report a survey investigating the prevalence of the Rdl mutant allele in mosquitoes from Laos, a country where mosquito-borne diseases such as malaria and dengue fever are health concerns. Anopheles and Aedes mosquitoes were collected from 12 provinces in Laos. Adult bioassays on Aedes aegypti (Linnaeus) (Diptera: Culicidae) and Aedes albopictus (Skuse) (Diptera: Culicidae) showed that all the populations tested were susceptible to dieldrin (4%) following WHO protocols. Exon 7 from a total of 791 mosquitoes was sequenced to identify the amino acid encoded for at 296 of RDL. Only one of these mosquitoes, Anopheles maculatus rampae Harbach and Somboon (Diptera: Culicidae) from Attapeu, carried the mutant allele being heterozygous for A296S. We therefore found a general lack of the Rdl mutant allele indicating that mosquitoes from Laos are not exposed to insecticides that act on the GABA receptor compared to mosquitoes in several other countries. Identifying the prevalence of the Rdl mutation may help inform the potential use of alternative insecticides that act on the GABA receptor should there be a need to replace pyrethroids in order to prevent/manage resistance.
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- 2020
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24. Malaria among foreign migrant workers in Savannakhet Province, Lao People’s Democratic Republic
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Phonepadith Khattignavong, Moritoshi Iwagami, Phonepadith Xangsayarath, Bouasy Hongvanthon, Paul T. Brey, Futoshi Nishimoto, Pheovaly Soundala, Jun Kobayashi, Daisuke Nonaka, Shigeyuki Kano, Tiengkham Pongvongsa, Savannakhet Provincial Health Department [Lao People’s Democratic Republic], Savannakhet Province [Lao People’s Democratic Republic], Science and Technology Research Partnership for Sustainable Development (SATREPS), Japan Agency for Medical Research and Development (AMED)-Japan International Cooperation Agency (JICA), University of the Ryukyus [Okinawa], National Center for Global Health and Medicine [Japan] (NCGM), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Ministry of Health [Laos], Nagasaki University, and This study was partly supported by a JICA/AMED SATREPS project for the 'Development of innovative research techniques in genetic epidemiology of malaria and other parasitic diseases in the Lao PDR for containing their expanding endemicity' and the Grant for National Center for Global Health and Medicine (28-4).
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medicine.medical_specialty ,lcsh:Arctic medicine. Tropical medicine ,lcsh:RC955-962 ,Vietnamese ,030231 tropical medicine ,LAO PEOPLE'S DEMOCRATIC REPUBLIC ,03 medical and health sciences ,0302 clinical medicine ,Malaria elimination ,parasitic diseases ,medicine ,030212 general & internal medicine ,Risk factor ,Socioeconomics ,Labor migration ,2. Zero hunger ,International migration ,Migrant workers ,Public health ,Research ,Public Health, Environmental and Occupational Health ,medicine.disease ,language.human_language ,3. Good health ,Malaria ,Help-seeking behavior ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Infectious Diseases ,Geography ,Vietnam ,Laos ,Tropical medicine ,language - Abstract
International audience; BackgroundAlthough mobile and migrant populations are considered an important group in malaria elimination settings, there is currently a lack of understanding about foreign migrant workers in the Lao People’s Democratic Republic (Lao PDR). The present study aimed to document the migration characteristics, positive rate of malaria infection, and preventive and treatment-seeking behavior for malaria among foreign migrant workers in the malaria-endemic districts of Savannakhet province, Lao PDR.MethodsA community-based survey was undertaken in four districts of Savannakhet province between February and June, 2015. Questionnaire-based interviews and blood examinations, including rapid diagnostic tests and PCR assays, were conducted with 391 migrant workers who were registered at local police departments.ResultsMost of the study participants were men (75.7%) and Vietnamese (92.6%). The median age (interquartile range) was 31 (25 to 41) years old. Most common occupation was factory worker (47.6%), followed by trader/shopkeeper (21.5%) and plantation worker/farmer (16.4%). The median length of stay (interquartile range) in the districts was 405 (183 to 1207) days. The majority of the participants (85.9%) had not worked in a province other than the study province, nor had the majority (92.6%) worked in a foreign country other than the Lao PDR. Although most of the participants (62.7%) reportedly used a bed net daily, these nets were mostly conventional untreated ones. No one tested positive for malaria. However, 10.0% of the participants reported a malaria-like illness episode that had occurred in the Lao PDR. The most common measure taken for the episode was to visit a hospital/health center in the Lao PDR, followed by conducting self-medication alone. Forty-one participants reported an experience of working in the forest while living in the Lao PDR.ConclusionsForeign migrant workers who are registered at local police departments are unlikely to play a major role in maintaining local transmissions and spreading drug-resistant malaria in the study province. However, some of them were involved in forest-related activities, suggesting that these workers are potentially at risk of malaria. The Lao National Malaria Control Program should educate foreign migrant workers about the risk of malaria when living in Lao PDR.
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- 2019
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25. Rilpivirine in HIV-1-positive women initiating pregnancy
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Frange, Pierre, Tubiana, Roland, Sibiude, Jeanne, Canestri, Ana, Arvieux, Cédric, Brunet-Cartier, Cécile, Cotte, Laurent, Reynes, Jacques, Mandelbrot, Laurent, Warszawski, Josiane, Le Chenadec, Jérôme, Crenn-Hebert, Catherine, Floch-Tudal, Corinne, Mazy, Fabienne, Joras, Marine, Meier, Françoise, Mortier, Emmanuel, Briquet, Catherine, Ichou, Houria, Marty, Laurence, Jabbarian, Hélène, Ceccaldi, Pierre-François, Villemant, Agnès, Zarouk, Virginie, Lefort, Agnès, Ben Salah, Mariam, Hittinger, Gilles, Chamouilli, Jean-Marc, Burle, Christian, Lafeuillade, Alain, Philip, Gisèle, Lambry, Véronique, Medus, Marie, Bachelard, Germaine, Malet, Martine, Dendale-Nguyen, Joëlle, Guimard, Thomas, Guimard, Karine, Brossier, Jean-Pierre, Perre, Philippe, Esnault, Jean-Luc, Aubry, Olivier, Leautez-Nainville, Sophie, Bonnenfant, Valerie, Laine, Laeticia, Martha, Sandrine-Anne, Maurel, Elise, Francoise, Michel, Barat, Muriel, Murger, Patricia, Rouha, Mahfoud, Lévy, Marc, Lumbroso, Philippe, Checoury, Alain, Sahadatu, Osseni, Perfezou, Pascale, Blondin, Gilles, Ansart, Séverine, De Saint Martin, Luc, Le Moine, Philippe, Duthé, Jean-Charles, Daniel, Corinne, Calvez, Christian, Boutaric, Emmanuelle, Rohan, Jennifer, Bauville, Estelle, Dupre, Christelle, Lotton, Pascal, Ouamara-digue, Enora, Poinsignon, Yves, Goussef, Marie, Grelier, Anne, Mousset, Gaetane, Cudeville, Corinne, Niault, Mathilde, Belzic, Isabelle, Moreau, Philippe, Le Coz, Marie-Françoise, Vaillant, Odile Luycx, Guerin-Duplessy, Anne, Mouton-Rioux, Virginie, De Morel, Philippe, Vitrat, Virginie, Tardif, Didier, Gaillat, Jacques, Vanderbergh, Anne, Braig, Suzanne, Clavere, Gaelle, Dehlinger-Paul, Marion, Mohamed, Khaled, Echard, Marie, Camus, Michel, Mulard, Catherine, Fontelonga, Marie-Agnès, Heller-Roussin, Brigitte, Winter, Cécile, Challier, Marion, Debruyne, Elise, Marcou, Valerie, Firtion, Ghislaine, Pannier, Emmanuelle, Costa, Myriam, Launay, Odile, Salmon-Ceron, Dominique, Belkacem, Touria, Bajawi, Youcef, Aubret, Valérie Raynal, Rivaux, Danièle, Elaoun, Neila, Allal, Lahcene, Djoubou, Sandrine, Rahli, Djamila, Moine, Agnès Bourgeois, Valentin, Morgane, Damond, Florence, Huri, Virginie, Vivier, Valérie, Yahia, Fatma Ait, Garrait, Valérie, Hau, Isabelle, Touboul, Claudine, Ratsimbazafy, Lanto, Boiron, Emilie, Elharrar, Brigitte, Labaune, Jean-Marc, Rudigoz, Rene-Charles, Brochier, Corinne, Galvan, Valérie, Ogoudjobi, Stanislas, Elleau, Christophe, Runel-Belliard, Camille, Pistone, Thierry, Fleury, Hervé, Horovitz, Jacques, Sandler, Boris, Roux, Denis, Ragnaud, Jean-Marie, Chabanier, Pierre, Brun, Jean-Luc, Delveaux, Sandrine, Muanza, Blandine, Diallo, Mama Doufari, Lamaury, Isabelle, Sow, Marie-Thérèse, Samar, Ketty, Carpentier, Bénédicte, Osman, Zafer, Dienga, Etienne, Seaume, Hervé, Ducrocq, Sarah, Bailly-Salin, Philippe, Da Silva, Christelle Dusart, Fayolas, Isabelle, Abbal, Julie, Simon-Toulza, Caroline, Truillet, Véronique, Bogner, Noëlle, Chiabrando, Julie, Armand, Evelyne, Cayla, Claudine, Chacé, Anne, Matheron, Isabelle, Richier, Laurent, Miantezila, Joe, Bry, Sandrine, Couderc, Sophie, Narcy, Catherine, Routier, Corinne, Nassar, Rania, Bouldouyre, Marie-Anne, Zakaria, Ahmed, Dauphin, Hélène, Goissen, Céline, Belloy, Marie, Delassus, Jean-Luc, Favret, Véronique, Nemeth, Céline, Partisani, Marialuisa, Entz-Werlé, Natacha, Langer, Bruno, Uettwiller, Françoise, Durand, Myriam, Partizani, MariaLuisa, Cheneau, Christine, Rey, David, Ebel, Edith, Fischer, Patricia, DAVID, Eric, Vayssière, Christophe, Weil, Michèle, Schmitt, Marie Paule, Nisand, Israël, Genet, Philippe, Brault, Dominique, Allisy, Christine, Gerbe, Juliette, Masse, Virginie, Wifaq, Bouchra, Courdavault, Laurence, Gabor, Petra, Tordjeman, Nathalie, Lebrette, Marie-Gisèle, Selleret, Lise, Samama, Déborah, Bolot, Pascal, Khuong-Josses, Marie-Aude, Amel, Mahdi, Bounan, Stéphane, Nourry, Christelle, Andris, Sabine, Blanche, Stephane, Driessen, Marine, Veber, Florence, Fischer, Alain, Rouzioux, Christine, Avettand-Fenoël, Véronique, Mahlaoui, Nizar, Mourey, Marie-Christine, Granier, Michèle, Devidas, Alain, Donnadieu, Anne-Claire, May, Adrien, Chabrol, Amélie, Chevojon, Pierre, Bellahcene, Chahrazede, Sanchez, Audrey, Malbrunot, Claire, Neizelien, Joelle, Agher, Nouara, Pluchart, Claire, Rouger, Christine, Dommergues, Marc, Bonmarchand, Manuela, Shneider, Luminata, Caby, Fabienne, Calin, Ruxandra-Oana, Blanc, Christine, Lupin, Catherine, Pauchard, Michèle, Yangui, Mohamed Amine, Roca, Didier, Todorova, Darina, Laurent, Juliette, Ferry, A., Deschaud, Martine, Blum, Laurent, Chambrin, Véronique, Labrune, Philippe, Clech, Laure, Raho-Moussa, Mariem, Pauly-Ravelly, Isolde, Jault, Thierry, Bouabdallah, Soufiane, Sanchez, Lydie, Sanchez, Anita, Johnson, Ama, Louchard, Agnès, Allouche, Claude, Pathe, Jean-Paul, Lachassine, Eric, Benoist, Laurence, Jeantils, Vincent, Delannoy, Catherine, Benbara, Amélie, Carbillon, Lionel, Borgne, Anne, Moreau, Laurence, PICARD, Fabienne, Karaoui, Leïla, Elbert, Véronique Lefevre, Balaz, Valérie, Bongain, André, Monpoux, Fabrice, Deville, Anne, Galiba, Eliane, Jabbar, Ahmed, Joutel, Martine, Schmidt, Jean-Luc, Decaux, Nathalie, Cravello, Ludovic, Errichiello, Katia, Hubert, Claire, Dollfus, Catherine, Hervé, François, Tabone, Marie-Dominique, Courcoux, Mary-France, Leverger, Guy, Kayem, Gilles, Schnurgier, Aurélie, Jensen, Aurore, Vaudre, Geneviève, Pinquier, Didier, Gromez, Alexis, Pinto-Cardoso, Gaelle, Faye, Albert, Borie, Constance, Levine, Martine, Matheron, Sophie, Marouts, Erianna Bellaton, Boissinot, Christine, Caseris, Marion, Pommelet, Virginie, Morau, Geneviève, Leveille, Sandrine, Boumediene, Marie Astride, Garion, Dominique, Peretti, Delphine, Fourcade, Corinne, Houllier, Marie, Jrad, Ikram, Bourdic, Katia, Monnier, Sylvie, Chirouze, Catherine, Proust, Aurélie, Catteau, Odile, Gardiennet, Quentin, Reliquet, Véronique, Winer, Norbert, Vaucel, Edouard, Rodallec, Audrey, Garnier-André, Elisabeth, Briandet, Claire, Brouard, Jacques, Goubin, Pascale, Beucher, Gaël, Dina, Julia, Demersay, Arnaud Chalvon, Tassi, Sylvie, Lavarenne, Gaelle, Rajguru, Mandovi, Messaoudi, Fabienne, Carré, Nathalie, Rajguru, Mandavi, Bobrie-Moyrand, Claire, Foucaud, Pierre, Bernard, Louis, Maakroun, Zoha, Bourgault, Olivier, Kebaili, Kamila, Bertrand, Yves, Alixe, Véronique, Boyer, Emeline, Billiemaz, Kareen, Fanget, Cécile, Ronat, Véronique, Lesauder, Catherine, Lavastre, Marie Laure, Moulin, Alice, Turquini, Marie-France, Colombani, Dominique, Belgodere, Danièle, Fialaire, Pascale, Proust, Stéphanie, Rehaiem, Sami, Mesnard, Louis, Werner, Evelyne, Dukiel, Nathalie, Desmergers, Baya, Blanc-Ruffat, Isabelle, Maraux, Barbara, Coursol, Anne, Castaneda, Julie, Etienne, Lise, Vintejoux, Emmanuelle, Lalande, Muriel, Segondy, Michel, Guigue, Nelly, De Gennes, Christiane, Clavel, Cyril, Cazassus, François, Walter, Véronique, Mazingue, Françoise, Hammou, Yamina, Lagree, Marion, Paquiez, Odile, D’angelo, Sophie, Boquet, Laurence, Ajana, Faiza, Hatchuel, Yves, Nahri, Imad, Zebelus, Jenny, Genet, Claire, Ducroix-Roubertou, Sophie, Aubrard, Yves, Constanty, Anne, Weinbreck, Pierre, Piet, Emilie, Jacquier, Françoise, Michaud, Christophe, Safwan, Hassan, Boutet, Arnaud, Grand-Courault, Carole, Autret, Fanny, Habibi, Fakher, Azria, Elie, Abdelhadi, Mohamed, Elenga, Narcisse, Bocket, Laurence, Taillet, Françoise, Palenzuela, Gilles, Khadly, Redouane, Pierronnet, Danielle, Dos-Santos, Emmanuelle, David, Selva, Makhloufi, Djamila, Brunel-Dalmas, Florence, Carbonnel-Delalande, Elisabeth, Chiarello, Pierre, Godinot, Matthieu, Gilbert, Sylvie, Massardier, Jérôme, Gauthier-Moulinier, Hélène, Fernandes, Elisabeth, Ranaivojaona, Sata, Chevry, Coralie, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Maladies infectieuses et tropicales [CHU Tenon], CHU Tenon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service des maladies infectieuses et réanimation médicale [Rennes] = Infectious Disease and Intensive Care [Rennes], CHU Pontchaillou [Rennes], Centre hospitalier universitaire de Nantes (CHU Nantes), Département de maladies infectieuses, Hospices Civils de Lyon (HCL), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques er émergentes (TransVIHMI), Institut de Recherche pour le Développement (IRD)-Université de Yaoundé I-Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Hôpital Louis Mourier - AP-HP [Colombes], Chirurgie Gynécologique et Obstétrique (CGO), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Département d'infectiologie [AP-HP Hôpital Louis Mourier, Colombes], Faculté de Médecine Paris-Diderot [Paris], Université Paris Diderot - Paris 7 (UPD7), Service de Médecine Interne [AP-HP, CHU Beaujon], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Beaujon [AP-HP], Service de médecine interne, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Hôpital Beaujon [AP-HP], Centre Hospitalier Intercommunal Toulon-La Seyne sur Mer - Hôpital Sainte-Musse, Service de pédiatrie, Centre Hospitalier Chalon-sur-Saône William Morey, CH de Lorient, Laboratoire de Traitement de l'Information Medicale (LaTIM), Université européenne de Bretagne - European University of Brittany (UEB)-Université de Brest (UBO)-Télécom Bretagne-Institut Mines-Télécom [Paris] (IMT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Département de Médecine Interne et Pneumologie [Brest] (DMIP - Brest), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Unité d'hémato-oncologie, CHU Toulouse [Toulouse], Pôle Médico-Chirurgical de Pédiatrie et de Génétique Clinique, Néonatologie, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Service de Gynécologie et Obstétrique [Rennes] = Gynaecology [Rennes], CH Bretagne Sud, Centre d'Investigations Biomédicales - Hématologie - Oncologie - Greffes (CIB-HOG), Centre d'Investigations Biomédicales - Hématologie - Oncologie - Greffes-Hopital St Louis, Centre Hospitalier Annecy-Genevois [Saint-Julien-en-Genevois], Service des Maladies Infectieuses, Centre Hospitalier de la Région d'Annecy (Pringy), University of Warwick [Coventry], Physiopathologie et Pharmacotoxicologie Placentaire Humaine (U1139), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Cochin [AP-HP], Université Paris Descartes - Paris 5 (UPD5), Service de Virologie [CHU Bichat], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Hôpital de la Croix-Rousse [CHU - HCL], Réseau périnatal Aurore, Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), CHU Bordeaux [Bordeaux], pôle gynécologie-obstétrique et médecine foetale, Laboratoire Rhéologie et Procédés (LRP), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA), Fédération des Maladies Infectieuses [Bordeaux], CHU de Bordeaux Pellegrin [Bordeaux], Centre de compétences des microangiopathies thrombotiques, department of pathology, university hospital, parakou, CHU Pointe-à-Pitre/Abymes [Guadeloupe], Service des Maladies Infectieuses et Tropicales[Point-à-Pitre], Service de Gynécologie-Obstétrique [Bicêtre], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Bicêtre, Hôpital Saint-Louis, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), CHU Strasbourg, Développement et physiopathologie de l'intestin et du pancréas, Institut National de la Santé et de la Recherche Médicale (INSERM), Service de gynécologie–obstétrique, CHU Strasbourg-Hôpital de Hautepierre [Strasbourg], CHRU Strasbourg, Département d'échographie et de Médecine fœtale, SIHCUS-CMCO, Service de Gynécologie-Obstétrique, CHI Poissy-Saint-Germain, Centre Hospitalier Victor Dupouy, Service d'informatique médicale et biostatistiques [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Hôpital d'Argenteuil, Centre Hospitalier de Saint-Denis [Ile-de-France], Hôpital Delafontaine, Recherche Epidémiologique en Santé Périnatale et Santé des Femmes et des Enfants (UMR_S 953), Université Paris-Sud - Paris 11 (UP11)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de microbilogie & unité d'immunologie, hématologie et rhumatologie pédiatriques, Developpement Normal et Pathologique du Système Immunitaire, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Chaire Médecine expérimentale (A. Fischer), Collège de France (CdF (institution)), Laboratoire de Virologie [CHU Necker], Université Sorbonne Paris Cité (USPC), IFR Necker-Enfants Malades (IRNEM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Médecine néonatale, Centre Hospitalier Sud Francilien, Service de pneumologie [Béclère], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Antoine Béclère [Clamart], Centre Hospitalier Universitaire de Reims (CHU Reims), Service de Gynécologie-Obstétrique, Maternité, Chirurgie Gynécologique [CHU Pitié-Salpêtrière], Service de médecine interne [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Croissance cellulaire, réparation et régénération tissulaires (CRRET), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Hôpital Antoine Béclère, Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre de Référence Maladies Héréditaires du Métabolisme Hépatique [Hôpital Antoine Béclère - APHP (CRMR), Service de rhumatologie [CHU Saint-Antoine], CHU Saint-Antoine [AP-HP], Service de gynécologie-obstétrique [Hôpital Jean Verdier], Université Paris 13 (UP13)-Hôpital Jean Verdier [AP-HP], Service de Gynécologie-Obstétrique-Reproduction, Hôpital l'Archet, Service d'Hémato-oncologie Pédiatrique [CHU Nice], Centre Hospitalier Universitaire de Nice (CHU Nice), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), CHU Trousseau [APHP], Service d'hématologie-immunologie-oncologie pédiatrique [CHU Trousseau], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Trousseau [APHP], Epidémiologie Clinique et Evaluation Economique Appliquées aux Populations Vulnérables (ECEVE (U1123 / UMR_S_1123)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AP-HP Hôpital universitaire Robert-Debré [Paris], Service des maladies infectieuses et tropicales, Service de pédiatrie générale, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Robert Debré-Université Paris Diderot - Paris 7 (UPD7), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Service de Gynécologie Obstétrique, Service de Médecine Interne et Immunologie clinique [AP-HP Hôpital Bicêtre], Hôpital Bicêtre, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), service de maladies infectieuses CHU J Minjoz Besancon, Service des maladies infectieuses et tropicales [CHU Nantes], Physiopathologie des Adaptations Nutritionnelles (PhAN), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Service de virologie [CHU Nantes], Service de Pédiatrie Enfants - Hématologie Oncologie [CHU de Dijon], Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Service de Pédiatrie Médicale [Caen], Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Service de Gynécologie-Obstétrique et Médecine de la Reproduction [CHU Caen], Service de Virologie [CHU Caen], CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Service de Médecine Interne et Maladies Infectieuses [Tours], Service d'hématologie : Immuno-Hématologie pédiatrique et transplantation de moelle osseuse, Hôpital Debrousse, CHU Saint-Etienne, Centre population et développement (CEPED - UMR_D 196), Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5), Médecine Néonatale et Réanimation Pédiatrique, CH René Dubos, Centre Hospitalier Universitaire de Nîmes (CHU Nîmes), Pathogénèse et contrôle des infections chroniques (PCCI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), Hôpital Jeanne de Flandres, Université de Lille, Droit et Santé-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Service des Maladies Infectieuses et Tropicales [Hôpital Gustave Dron, Tourcoing], Centre Hospitalier Gustave Dron [Tourcoing], 'Personal Protection Against Vectors' working group (PPAV), PPAV working group, Service des Maladies infectieuses et tropicales [CHU Limoges], CHU Limoges, Equipe de Recherche Médicale Appliquée (ERMA), Université de Limoges (UNILIM)-CHU Limoges-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503), CHU Annecy, Service de Pédiatrie, Centre hospitalier de Saint-Nazaire, EA 3593 Université des Antilles et de la Guyane, Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française]-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Laboratoire de virologie [CHU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Service de Maladies Infectieuses et Tropicales [Hôpital de la Croix-Rousse - HCL], Service d'immunologie, Hospices Civils de Lyon (HCL)-Hôpital Edouard Herriot [CHU - HCL], Hôpital Femme Mère Enfant [CHU - HCL] (HFME), Centre Hospitalier de Basse-Terre, Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques et émergentes (TransVIHMI), Université de Rennes (UR), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Collège de France - Chaire Médecine expérimentale (A. Fischer), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), ANRS CO1/CO11, French national, Viral Hepatitis, Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris-Sud - Paris 11 (UP11)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Service des maladies infectieuses et tropicales [CHU Tenon], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Institut de Recherche pour le Développement (IRD)-Université de Yaoundé I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), Université européenne de Bretagne - European University of Brittany (UEB)-Télécom Bretagne-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)-Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Mines-Télécom [Paris] (IMT), Hôpital des Enfants, CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse], Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Sud - Paris 11 (UP11), CH Evry-Corbeil-CH Evry-Corbeil, CH Evry-Corbeil, Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Jean Verdier [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris 13 (UP13), Université Paris Diderot - Paris 7 (UPD7)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Laboratoire d'Electrotechnique de Lyon (LEL), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon, Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Université de Limoges (UNILIM)-CHU Limoges, Institut National de la Santé et de la Recherche Médicale (INSERM)-AP-HP Hôpital universitaire Robert-Debré [Paris], Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )-Université de Montpellier (UM)
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rilpivirine ,viral suppression ,hiv ,hiv-1 ,pregnancy ,conception ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,virology - Abstract
International audience; BackgroundSafety data about rilpivirine use during pregnancy remain scarce, and rilpivirine plasma concentrations are reduced during second/third trimesters, with a potential risk of viral breakthroughs. Thus, French guidelines recommend switching to rilpivirine-free combinations (RFCs) during pregnancy.ObjectivesTo describe the characteristics of women initiating pregnancy while on rilpivirine and to compare the outcomes for virologically suppressed subjects continuing rilpivirine until delivery versus switching to an RFC.MethodsIn the ANRS-EPF French Perinatal cohort, we included women on rilpivirine at conception in 2010–18. Pregnancy outcomes were compared between patients continuing versus interrupting rilpivirine. In women with documented viral suppression (
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26. Travel-associated chikungunya acquired in Myanmar in 2019
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Guido Calleri, Marta Díaz-Menéndez, Alfred Lennart Bissinger, Spinello Antinori, Camilla Rothe, Jonathan D. Alpern, Emanuele Nicastri, Patricia Schlagenhauf, Mugen Ujiie, Elena Trigo Esteban, Denis Malvy, Davidson H. Hamer, Federico Gobbi, Alexandre Duvignaud, Emilie Javelle, Marc Grandadam, Takato Nakamoto, University of Zurich, Díaz-Menéndez, Marta, Epidémiologie et Biostatistique [Bordeaux], Université Bordeaux Segalen - Bordeaux 2-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM), Infectious Diseases Unit, Istituto Nazionale di Malattie Infettive 'Lazzaro Spallanzani' (INMI), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Université Paris Descartes - Paris 5 (UPD5), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), and Institut de Recherche Biomédicale des Armées (IRBA)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)
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Male ,0301 basic medicine ,Epidemiology ,Myanmar ,medicine.disease_cause ,Chikungunya virus disease ,Disease Outbreaks ,0302 clinical medicine ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Travel medicine ,Chikungunya ,ComputingMilieux_MISCELLANEOUS ,Travel ,[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases ,Middle Aged ,Arthralgia ,3. Good health ,Geography ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Chikungunya Virus Infection ,surveillance ,Female ,Chikungunya virus ,Rapid Communication ,Adult ,medicine.medical_specialty ,Fever ,030106 microbiology ,030231 tropical medicine ,610 Medicine & health ,Mosquito Vectors ,Virus ,03 medical and health sciences ,[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system ,Virology ,medicine ,Humans ,[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,Aged ,Public Health, Environmental and Occupational Health ,Outbreak ,10060 Epidemiology, Biostatistics and Prevention Institute (EBPI) ,2739 Public Health, Environmental and Occupational Health ,Exanthema ,travel medicine ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,2406 Virology ,Chikungunya Fever ,Sentinel Surveillance ,2713 Epidemiology - Abstract
Eighteen cases of chikungunya virus infection in travellers returning from Myanmar were reported to the GeoSentinel Surveillance Network, its subnetwork EuroTravNet and TropNet in 2019, reflecting an ongoing local outbreak. This report reinforces the importance of travellers as sentinels of emerging arboviral outbreaks and highlights the importance of vigilance for imported cases, due to the potential for dissemination of the virus into areas with competent local vectors and conducive environmental conditions.
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27. Rapid genotyping protocol to improve dengue virus serotype 2 survey in Lao PDR
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Sitsana Keosenhom, Elodie Calvez, Phaithong Bounmany, Souksakhone Viengphouthong, Charlotte Balière, Somphavanh Somlor, Valérie Caro, Marc Grandadam, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Environnement et Risques infectieux - Environment and Infectious Risks (ERI), Institut Pasteur [Paris], MG received funding from the Agence Française de Développement grant n° CZZ 2146 01 (Ecomore2 project)., and Institut Pasteur [Paris] (IP)
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0301 basic medicine ,Serotype ,RNA viruses ,Viral Diseases ,Genetic Screens ,Time Factors ,Genotyping Techniques ,Urban Population ,Molecular biology ,viruses ,Gene Identification and Analysis ,Gene Sequencing ,Artificial Gene Amplification and Extension ,Dengue virus ,medicine.disease_cause ,Pathology and Laboratory Medicine ,MESH: Dengue Virus ,Biochemistry ,Polymerase Chain Reaction ,Dengue fever ,Dengue Fever ,0302 clinical medicine ,Sequencing techniques ,Medical Conditions ,Surveys and Questionnaires ,Genotype ,Medicine and Health Sciences ,Ethnicities ,DNA sequencing ,Data Management ,Multidisciplinary ,virus diseases ,Phylogenetic Analysis ,3. Good health ,Phylogenetics ,MESH: Urban Population ,Infectious Diseases ,Laos ,Medical Microbiology ,Viral Pathogens ,Viruses ,Lao People ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Medicine ,Pathogens ,Research Article ,Neglected Tropical Diseases ,medicine.medical_specialty ,Computer and Information Sciences ,First line ,Science ,030231 tropical medicine ,Biology ,Microbiology ,03 medical and health sciences ,Asian People ,MESH: Genotyping Techniques ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,medicine ,Genetics ,Humans ,Evolutionary Systematics ,Serotyping ,MESH: Surveys and Questionnaires ,Genotyping ,Microbial Pathogens ,Taxonomy ,[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health ,Evolutionary Biology ,Plasma Proteins ,MESH: Humans ,Molecular epidemiology ,Flaviviruses ,Public health ,MESH: Time Factors ,Organisms ,Biology and Life Sciences ,Proteins ,MESH: Serotyping ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Reverse Transcriptase-Polymerase Chain Reaction ,Dengue Virus ,biochemical phenomena, metabolism, and nutrition ,medicine.disease ,Tropical Diseases ,Virology ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Research and analysis methods ,030104 developmental biology ,Molecular biology techniques ,MESH: Laos ,People and Places ,Population Groupings ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
International audience; Dengue fever is one of the major public health problems in Lao PDR. Over the last decade, dengue virus (DENV) epidemics were characterized by a novel predominant serotype accompanied by at least two other serotypes. Since 2008, DENV-2 circulated at a low level in Lao PDR but its epidemiologic profile changed at the end of 2018. Indeed, the number of confirmed DENV-2 cases suddenly increased in October 2018 and DENV-2 became predominant at the country level in early 2019. We developed a Genotype Screening Protocol (GSP) to determine the origin(s) of the Lao DENV-2 and study their genetic polymorphism. With a good correlation with full envelope gene sequencing data, this molecular epidemiology tool evidence the co-circulation of two highly polymorphic DENV-2 genotypes, i.e. Asian I and Cosmopolitan genotypes, over the last five years, suggesting multiple introductions of DENV-2 in the country. GSP approach provides relevant first line information that may help countries with limited laboratory resources to reinforce their capabilities to DENV-2 and to follow the epidemics progresses and assess situations at the regional level.
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28. High diversity of mosquito vectors in Cambodian primary schools and consequences for arbovirus transmission
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Sony Yean, Sébastien Boyer, Sébastien Marcombe, Didier Fontenille, Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur du Laos, and The study was supported by ECOMORE 2 project, coordinated by Institut Pasteur and financially supported by AFD (Agence Française pour le Développement).
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RNA viruses ,Culex vishnui ,Viral Diseases ,[SDV]Life Sciences [q-bio] ,Social Sciences ,Disease Vectors ,Pathology and Laboratory Medicine ,Mosquitoes ,Dengue Fever ,Dengue fever ,Dengue ,Geographical Locations ,0302 clinical medicine ,Sociology ,Medicine and Health Sciences ,030212 general & internal medicine ,Child ,Socioeconomics ,2. Zero hunger ,Schools ,Multidisciplinary ,biology ,Anopheles ,Eukaryota ,virus diseases ,Biodiversity ,3. Good health ,Insects ,Infectious Diseases ,Geography ,Medical Microbiology ,Child, Preschool ,Viral Pathogens ,Viruses ,Medicine ,Pathogens ,Viral Vectors ,Cambodia ,Research Article ,Neglected Tropical Diseases ,Asia ,Adolescent ,Arthropoda ,Culex ,Science ,030231 tropical medicine ,education ,Mosquito Vectors ,Arbovirus Infections ,Aedes Aegypti ,Microbiology ,Arbovirus ,Education ,03 medical and health sciences ,Virology ,parasitic diseases ,Parasitic Diseases ,medicine ,Humans ,Animals ,Encephalitis, Japanese ,Microbial Pathogens ,Aedes ,Flaviviruses ,Organisms ,Biology and Life Sciences ,Dengue Virus ,Japanese encephalitis ,Tropical Diseases ,biology.organism_classification ,medicine.disease ,Invertebrates ,Culex quinquefasciatus ,Malaria ,Insect Vectors ,Species Interactions ,People and Places ,Arboviruses ,Viral Transmission and Infection - Abstract
International audience; Only few data exist in Cambodia on mosquito diversity and their potential role as vectors. Many arboviruses, such as dengue and Japanese encephalitis, are endemic and mostly affect children in the country. This research sets out to evaluate vector relative abundance and diversity in primary schools in Cambodia in an attempt to explain the apparent burden of dengue fever, severe dengue (DEN), Japanese encephalitis (JE), other arboviral diseases and malaria among children, 15 years and under, attending selected primary schools through vector surveys. Entomological surveys were implemented in primary schools in two provinces of Cambodia to assess the potential risk of exposure of schoolchildren to mosquito vector species. Light traps and BG traps were used to collect adult mosquitoes in 24 schools during the rainy and dry seasons of 2017 and 2018 in Kampong Cham and Tboung Khmum provinces. A total of 61 species were described, including Aedes, Culex and Anopheles species. The relative abundance and biodiversity of mosquito species were dependent on the month and school. Of the 37,725 mosquitoes caught during the study, three species accounted for three-quarters of the relative abundance: Culex vishnui, Anopheles indefinitus and Culex quinquefasciatus. More importantly, nearly 90% of the mosquitoes caught in the schools were identified as potential vectors of pathogens including Japanese encephalitis, dengue, and malaria parasites. Our results showed that schools in Cambodia represent a risk for vector-borne disease transmission and highlight the importance of implementing vector control in schools in Cambodia to decrease the risk of transmission.
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29. Potentiel du moustique Aedes malayensis comme vecteur d’arbovirus en Asie du Sud-Est
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Miot, Elliott, Ecole Doctorale Complexité du Vivant (ED515), Sorbonne Université (SU), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Interactions Virus-Insectes - Insect-Virus Interactions (IVI), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Sorbonne Université, Louis Lambrechts, and Paul Timothy Brey
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Arbovirus ,Compétence vectorielle ,Asie du Sud-Est ,Fièvre jaune ,Aedes malayensis ,Host-vector contact ,South East Asia ,Bridge vector ,Dengue ,Culicidae ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Host-seeking behavior ,Contact hôte-vecteur ,Yellow fever ,Vector competence - Abstract
Many emerging arthropod-borne viruses (arboviruses) such as dengue virus (DENV) and yellow fever virus (YFV) originated in sylvatic cycles and have emerged among humans through spillover transmission by mosquito species that ‘bridge’ sylvatic and human transmission cycles. These bridge vectors can also mediate ‘spillback’ transmission of arboviruses from humans into novel sylvatic cycles. This PhD focused on Aedes malayensis, a mosquito species widely distributed in South East Asia, to assess its potential as an arbovirus vector. We identified Ae. malayensis for the first time in Laos during mosquito surveys conducted in a forested area of the Nakai Nam Theun National Protected Area (NNT NPA). Using field-based human-baited traps, we found that Ae. malayensis engaged in human-biting behavior and therefore could act as bridge vector in the NNT NPA. In laboratory conditions, this sylvatic population of Ae. malayensis displayed a relatively low vector competence for DENV and YFV and a lack of detectable attraction to human odor. However, vector competence assays and a human-baited trap survey showed that a peridomestic Ae. malayensis population in Singapore was competent for YFV and engaged in contact with humans. Overall, this PhD work highlighted that ancillary vectors should not be overlooked to fully assess the risk of arbovirus emergence.; De nombreux virus transmis par les arthropodes (arbovirus), tels que ceux de la dengue (DENV) et de la fièvre jaune (YFV), circulaient à l’origine dans des cycles selvatiques et ont émergé chez l’Homme via des moustiques « bridge vectors » qui connectent les cycles de transmission selvatiques et humains. Ces « bridge vectors » peuvent aussi par transfert inverse établir de nouveaux cycles selvatiques. Cette thèse a évalué le potentiel de vecteur d’arbovirus d’un moustique répandu en Asie du sud-est, Aedes malayensis. Nous avons identifié Ae. malayensis pour la première fois au Laos lors de captures de moustiques dans une forêt de la réserve de Nakai Nam Theun. En utilisant des pièges à appâts humains sur le terrain, nous avons observé qu’Ae. malayensis pouvait piquer l’Homme et donc potentiellement agir comme « bridge vector ». En laboratoire, cette population selvatique d’Ae. malayensis a montré une faible compétence vectorielle relative pour DENV et YFV, et une absence d’attraction détectable pour l’odeur humaine. Cependant, des tests de compétence vectorielle et de pièges à appâts humains ont révélé qu’une population péri-domestique d’Ae. malayensis à Singapour était compétente pour YFV et entrait en contact avec l’Homme. Au final, ce travail de doctorat a souligné l’importance de ne pas négliger les vecteurs secondaires dans l’évaluation du risque d’émergence des arbovirus.
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- 2019
30. A peridomestic Aedes malayensis population in Singapore can transmit yellow fever virus
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Anna-Bella Failloux, Cheong H. Tan, Lee C. Ng, Stéphanie Dabo, Paul T. Brey, Julien Pompon, Fabien Aubry, Sébastien Marcombe, Ian H. Mendenhall, Louis Lambrechts, Elliott F. Miot, Interactions Virus-Insectes - Insect-Virus Interactions (IVI), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Duke-NUS Medical School [Singapore], Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), EFM was supported by a Calmette and Yersin doctoral fellowship of the Institut Pasteur International Network. This work was funded by Agence Nationale de la Recherche (grant ANR-17-ERC2-0016-01 to LL), the French Government’s Investissement d’Avenir program Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID to LL), the European Union’s Horizon 2020 research and innovation program under ZikaPLAN grant agreement no. 734584 (to LL), and the City of Paris Emergence(s) program in Biomedical Research (to LL)., We thank Catherine Lallemand for assistance with mosquito rearing and three anonymous reviewers for constructive comments on an earlier version of the manuscript. We are grateful to the blood donor volunteers for participation in this study and the ICAReB staff for its support. We thank the National Park Board Singapore for their permission to collect in their parks and Jeffrey C. Hertz for his support and advice with the mosquito collections., ANR-17-ERC2-0016,GxG,Base génétique de la spécificité génotype-génotype dans l'interaction naturelle entre un virus et son insecte vecteur(2017), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 734584,ZikaPLAN, and Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris]
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RNA viruses ,0301 basic medicine ,Viral vectors ,Physiology ,RC955-962 ,Disease Vectors ,Pathology and Laboratory Medicine ,medicine.disease_cause ,Mosquitoes ,Dengue fever ,Geographical Locations ,0302 clinical medicine ,Aedes aegypti ,Aedes ,Arctic medicine. Tropical medicine ,Medicine and Health Sciences ,Chikungunya ,education.field_of_study ,Singapore ,biology ,Yellow fever ,Eukaryota ,Body Fluids ,3. Good health ,Insects ,Infectious Diseases ,Blood ,Medical Microbiology ,Viral Pathogens ,Host-Pathogen Interactions ,Viruses ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Female ,Public aspects of medicine ,RA1-1270 ,Yellow fever virus ,Anatomy ,Pathogens ,Chikungunya virus ,Research Article ,Asia ,Arthropoda ,Alphaviruses ,030231 tropical medicine ,Population ,Zoology ,Mosquito Vectors ,Microbiology ,Arbovirus ,Togaviruses ,03 medical and health sciences ,Virology ,Yellow Fever ,Infectious disease control ,medicine ,Animals ,Humans ,Saliva ,education ,Microbial Pathogens ,Organisms ,Public Health, Environmental and Occupational Health ,Biology and Life Sciences ,Outbreak ,medicine.disease ,biology.organism_classification ,Invertebrates ,Insect Vectors ,Species Interactions ,030104 developmental biology ,Vector (epidemiology) ,People and Places ,Viral Transmission and Infection ,Arboviruses - Abstract
The case-fatality rate of yellow fever virus (YFV) is one of the highest among arthropod-borne viruses (arboviruses). Although historically, the Asia-Pacific region has remained free of YFV, the risk of introduction has never been higher due to the increasing influx of people from endemic regions and the recent outbreaks in Africa and South America. Singapore is a global hub for trade and tourism and therefore at high risk for YFV introduction. Effective control of the main domestic mosquito vector Aedes aegypti in Singapore has failed to prevent re-emergence of dengue, chikungunya and Zika viruses in the last two decades, raising suspicions that peridomestic mosquito species untargeted by domestic vector control measures may contribute to arbovirus transmission. Here, we provide empirical evidence that the peridomestic mosquito Aedes malayensis found in Singapore can transmit YFV. Our laboratory mosquito colony recently derived from wild Ae. malayensis in Singapore was experimentally competent for YFV to a similar level as Ae. aegypti controls. In addition, we captured Ae. malayensis females in one human-baited trap during three days of collection, providing preliminary evidence that host-vector contact may occur in field conditions. Finally, we detected Ae. malayensis eggs in traps deployed in high-rise building areas of Singapore. We conclude that Ae. malayensis is a competent vector of YFV and re-emphasize that vector control methods should be extended to target peridomestic vector species., Author summary Yellow fever is a dreadful disease caused by a mosquito-borne virus circulating in Africa and South America. Historically, the Asia-Pacific region has remained free of yellow fever but the ever increasing influx of travelers puts places such as Singapore at unprecedented risk of yellow fever virus introduction. The present study characterized the potential contribution of a mosquito species called Aedes malayensis to yellow fever virus transmission in Singapore. Aedes malayensis breeds in urban parks of Singapore and is suspected to have participated in the resurgence of other mosquito-borne diseases such as dengue because it is not targeted by current mosquito control measures. Not only was Ae. malayensis able to experimentally acquire and transmit yellow fever virus, but it was also found to engage in contact with humans in a field situation. This empirical evidence indicates that Ae. malayensis is a competent vector of yellow fever virus and should be targeted by mosquito control programs.
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31. Cell-Fusing Agent Virus Reduces Arbovirus Dissemination in Aedes aegypti Mosquitoes In Vivo
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Louis Lambrechts, Van-Mai Cao-Lormeau, Sarah H. Merkling, Isabelle Moltini-Conclois, Fanny Delaigue, Elliott F. Miot, Fabien Aubry, Artem Baidaliuk, Laura B. Dickson, Stéphanie Dabo, Sebastian Lequime, Interactions Virus-Insectes - Insect-Virus Interactions (IVI), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Collège doctoral [Sorbonne universités], Sorbonne Université (SU), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Institut Louis Malardé [Papeete] (ILM), Institut de Recherche pour le Développement (IRD), This work was supported by the Agence Nationale de la Recherche (grants ANR-16-CE35-0004-01 and ANR-17-ERC2-0016-01), the French Government’s Investissement d’Avenir program Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID), the European Union’s Horizon 2020 research and innovation program under ZikaPLAN grant agreement no. 734584, and the City of Paris Emergence(s) Program in Biomedical Research., We thank Catherine Lallemand for assistance with mosquito rearing. We are grateful to Carla Saleh, Marie Flamand, Yasutsugu Suzuki, Laura Levi, Clément Dubois, and two anonymous reviewers for their insights. We thank Thanyalak Fansiri and Alongkot Ponlawat for the initial field sampling of mosquitoes in Thailand. We are grateful to the blood donor volunteers for participation in this study and the ICAReB staff for its support., ANR-16-CE35-0004,MOSQUIBIOTA,Contribution de la diversité bactérienne intestinale à la capacité vectorielle d'Aedes aegypti(2016), ANR-17-ERC2-0016,GxG,Base génétique de la spécificité génotype-génotype dans l'interaction naturelle entre un virus et son insecte vecteur(2017), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 734584,ZikaPLAN, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Collège Doctoral
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viruses ,insect-specific virus ,viral interference ,Dengue virus ,Virus Replication ,medicine.disease_cause ,Dengue fever ,Zika virus ,Dengue ,Aedes aegypti ,Aedes ,Spotlight ,Viral Interference ,Phylogeny ,0303 health sciences ,biology ,Zika Virus Infection ,virus diseases ,3. Good health ,Flavivirus ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Immunology ,Insect Viruses ,Mosquito Vectors ,Microbiology ,Arbovirus ,Virus ,Cell Line ,superinfection ,03 medical and health sciences ,Virology ,medicine ,Animals ,Humans ,030304 developmental biology ,030306 microbiology ,fungi ,Zika Virus ,Dengue Virus ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,medicine.disease ,coinfection ,arbovirus ,Genetic Diversity and Evolution ,Insect Science ,Arboviruses - Abstract
The mosquito Aedes aegypti carries several arthropod-borne viruses (arboviruses) that are pathogenic to humans, including dengue and Zika viruses. Interestingly, A. aegypti is also naturally infected with insect-only viruses, such as cell-fusing agent virus. Although interactions between cell-fusing agent virus and dengue virus have been documented in mosquito cells in culture, whether wild strains of cell-fusing agent virus interfere with arbovirus transmission by live mosquitoes was unknown. We used an experimental approach to demonstrate that cell-fusing agent virus infection reduces the propagation of dengue and Zika viruses in A. aegypti mosquitoes. These results support the idea that insect-only viruses in nature can modulate the ability of mosquitoes to carry arboviruses of medical significance and that they could possibly be manipulated to reduce arbovirus transmission., Aedes aegypti mosquitoes are the main vectors of arthropod-borne viruses (arboviruses) of public health significance, such as the flaviviruses dengue virus (DENV) and Zika virus (ZIKV). Mosquitoes are also the natural hosts of a wide range of viruses that are insect specific, raising the question of their influence on arbovirus transmission in nature. Cell-fusing agent virus (CFAV) was the first described insect-specific flavivirus, initially discovered in an A. aegypti cell line and subsequently detected in natural A. aegypti populations. It was recently shown that DENV and the CFAV strain isolated from the A. aegypti cell line have mutually beneficial interactions in mosquito cells in culture. However, whether natural strains of CFAV and DENV interact in live mosquitoes is unknown. Using a wild-type CFAV isolate recently derived from Thai A. aegypti mosquitoes, we found that CFAV negatively interferes with both DENV type 1 and ZIKV in vitro and in vivo. For both arboviruses, prior infection by CFAV reduced the dissemination titer in mosquito head tissues. Our results indicate that the interactions observed between arboviruses and the CFAV strain derived from the cell line might not be a relevant model of the viral interference that we observed in vivo. Overall, our study supports the hypothesis that insect-specific flaviviruses may contribute to reduce the transmission of human-pathogenic flaviviruses. IMPORTANCE The mosquito Aedes aegypti carries several arthropod-borne viruses (arboviruses) that are pathogenic to humans, including dengue and Zika viruses. Interestingly, A. aegypti is also naturally infected with insect-only viruses, such as cell-fusing agent virus. Although interactions between cell-fusing agent virus and dengue virus have been documented in mosquito cells in culture, whether wild strains of cell-fusing agent virus interfere with arbovirus transmission by live mosquitoes was unknown. We used an experimental approach to demonstrate that cell-fusing agent virus infection reduces the propagation of dengue and Zika viruses in A. aegypti mosquitoes. These results support the idea that insect-only viruses in nature can modulate the ability of mosquitoes to carry arboviruses of medical significance and that they could possibly be manipulated to reduce arbovirus transmission.
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32. Prevalence of G6PD Viangchan variant in malaria endemic areas in Lao PDR: an implication for malaria elimination by 2030
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Phonepadith Khattignavong, Pheovaly Soundala, Sengdeuane Keomalaphet, Paul T. Brey, Phoyphaylinh Prasayasith, Lavy Lorpachan, Ken Ing Cherng Ong, Tiengkham Pongvongsa, Shigeyuki Kano, Bouasy Hongvanthong, Hitomi Araki, Moritoshi Iwagami, Phonepadith Xangsayalath, Masamine Jimba, The University of Tokyo (UTokyo), Science and Technology Research Partnership for Sustainable Development (SATREPS), Japan Agency for Medical Research and Development (AMED)-Japan International Cooperation Agency (JICA), National Center for Global Health and Medicine [Japan] (NCGM), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Ministry of Health [Laos], Savannakhet Provincial Health Department [Lao People’s Democratic Republic], Savannakhet Province [Lao People’s Democratic Republic], and This study was financially supported by the JICA/AMED SATREPS project for the 'Development of innovative research techniques in the genetic epidemiology of malaria and other parasitic diseases in the Lao PDR for containing their expanding endemicity.'
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Male ,Rural Population ,Veterinary medicine ,MESH: Sequence Analysis, DNA ,Primaquine ,Plasmodium vivax ,MESH: Disease Eradication ,MESH: Genotype ,0302 clinical medicine ,MESH: Aged, 80 and over ,MESH: Rural Population ,hemic and lymphatic diseases ,MESH: Child ,Prevalence ,030212 general & internal medicine ,Child ,Aged, 80 and over ,MESH: Aged ,MESH: Middle Aged ,biology ,Glucose-6-phosphate dehydrogenase deficiency ,MESH: Infant, Newborn ,Middle Aged ,MESH: Primaquine ,Haemolysis ,MESH: Hemolysis ,MESH: Infant ,3. Good health ,MESH: Glucosephosphate Dehydrogenase Deficiency ,Infectious Diseases ,Laos ,MESH: Young Adult ,Child, Preschool ,Female ,medicine.drug ,Adult ,medicine.medical_specialty ,lcsh:Arctic medicine. Tropical medicine ,Adolescent ,Genotype ,lcsh:RC955-962 ,030231 tropical medicine ,MESH: Glucosephosphate Dehydrogenase ,Malaria elimination ,Glucosephosphate Dehydrogenase ,Hemolysis ,lcsh:Infectious and parasitic diseases ,Young Adult ,03 medical and health sciences ,parasitic diseases ,Malaria, Vivax ,medicine ,Humans ,lcsh:RC109-216 ,Disease Eradication ,MESH: Prevalence ,Aged ,MESH: Adolescent ,MESH: Humans ,business.industry ,Research ,MESH: Child, Preschool ,Infant, Newborn ,Infant ,MESH: Malaria, Vivax ,MESH: Adult ,Sequence Analysis, DNA ,medicine.disease ,biology.organism_classification ,MESH: Male ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Glucosephosphate Dehydrogenase Deficiency ,Parasitology ,MESH: Laos ,Tropical medicine ,business ,MESH: Female ,Malaria - Abstract
Background Primaquine is effective against the latent liver stage of Plasmodium vivax. Eliminating the latent liver stage of P. vivax is one of the necessary conditions to achieve the goal of malaria elimination in Lao People’s Democratic Republic (PDR) by 2030. However, people with glucose-6-phosphate dehydrogenase (G6PD) deficiency are at risk of haemolysis when ingesting primaquine. The aim of this study was to detect the prevalence of the G6PD Viangchan variant, which is said to be common in Lao PDR and which can result in severe haemolysis in patients exposed to primaquine. Methods Blood samples were collected from villagers in three malaria endemic provinces: Champasak and Savannakhet in the south, and Phongsaly in the north. Each blood sample was semi-quantitatively assayed for G6PD enzyme activity using the G6PD Assay Kit-WST Lyophilized (DOJINDO Laboratories, Japan). Blood samples that were found to be G6PD deficient were sequenced to detect G6PD Viangchan mutation. Results In total, 2043 blood samples were collected from Phongsaly (n = 426, 20.9%), Savannakhet (n = 924, 45.2%), and Champasak (n = 693, 33.9%) provinces in Lao PDR from 2016 to 2017. Of these, 964 (47.2%) were taken from male villagers and 1079 (52.8%) were taken from female villagers. G6PD Viangchan mutation was not detected in Phongsaly province in this study. In Savannakhet province, 48 of the 924 samples (45 males, 3 females) had the G6PD Viangchan mutation (n = 48, 5.2%). In Champasak province, 42 of the 693 samples (18 males, 24 females) had the G6PD Viangchan mutation (n = 42, 6.1%). Conclusions G6PD Viangchan variant, which can cause severe haemolysis in the carrier when exposed to primaquine, was detected among 6.1% of the villagers in Champasak and 5.2% in Savannakhet but not in Phongsaly in this study. G6PD Viangchan variant might be common in the south of Laos but not so in the north. In the north, other G6PD deficiency variants might be more prevalent. However, in order not to overlook anyone and ensure a safe primaquine therapy for people living in malaria endemic areas in Lao PDR, G6PD testing is necessary. Electronic supplementary material The online version of this article (10.1186/s12936-019-2715-0) contains supplementary material, which is available to authorized users.
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33. Malaria prevalence, knowledge, perception, preventive and treatment behavior among military in Champasak and Attapeu provinces, Lao PDR: a mixed methods study
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Sengchanh Kounnavong, Phoutnalong Vilay, Paul T. Brey, Phosadeth Senamonty, Moritoshi Iwagami, Malayvanh Lao, Shigeyuki Kano, Daisuke Nonaka, Jun Kobayashi, Bouasy Hongvanthong, Paul Michael R. Hernandez, Ketkesone Phrasisombath, University of the Ryukyus [Okinawa], Ministry of Health [Laos], Science and Technology Research Partnership for Sustainable Development (SATREPS), Japan Agency for Medical Research and Development (AMED)-Japan International Cooperation Agency (JICA), Ministry of Defense [Vientiane, Laos], National Center for Global Health and Medicine [Japan] (NCGM), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), University of the Philippines (UP System), University of Health Sciences [Vientiane, Laos] (UHS), and Department of Global Health, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Japan and Japan International Cooperation Agency (JICA)/ Science and Technology Research Partnership for Sustainable Development program (SATREPS) project for 'Development of Innovative Research Technique Epidemiology of Malaria and other Parasitic Diseases' supported the study.
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medicine.medical_specialty ,lcsh:Arctic medicine. Tropical medicine ,lcsh:RC955-962 ,030231 tropical medicine ,Plasmodium vivax ,Preventive measure ,03 medical and health sciences ,0302 clinical medicine ,Military ,Environmental health ,Treatment behavior ,parasitic diseases ,Prevalence ,medicine ,030212 general & internal medicine ,biology ,business.industry ,Research ,Public health ,Public Health, Environmental and Occupational Health ,Anopheles ,Plasmodium falciparum ,biology.organism_classification ,medicine.disease ,Malaria ,3. Good health ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Military personnel ,Knowledge ,Infectious Diseases ,Laos ,Vector (epidemiology) ,Perception ,Health education ,business - Abstract
International audience; BackgroundMalaria is a major health problem in Lao People’s Democratic Republic (Lao PDR) with high transmission in remote and forest areas, particularly in the South. The military is at risk of malaria infection especially those deployed in forest areas. This study determined the prevalence of malaria infection and assessed knowledge, perception, and preventive and treatment behavior regarding malaria among military personnel in two southern provinces in Lao PDR.MethodsQuantitative and qualitative approaches were undertaken in Champasak and Attapeu provinces in 2017. From 313 military personnel, quantitative data were collected through questionnaire-based interviews and blood samples used for parasite detection by polymerase chain reaction (PCR). Qualitative data were collected through 7 focus group discussions and 17 in-depth interviews among 49 military personnel. Fisher’s exact test and Mann-Whitney U test were used to assess the association between malaria infection and participant characteristics. Content analysis for qualitative data was performed to explore perception and treatment behaviors regarding malaria.ResultsThe prevalence of malaria infection was 11.2% (Plasmodium falciparum: 1.3%, Plasmodium vivax: 9.3% and mixed infections: 0.6%). Many participants understood that malaria is transmitted through mosquito bites, although they did not necessarily know the name of vector mosquitoes (Anopheles). Surprisingly, more than a half also believed that malaria is transmitted through drinking stream water. One-third of the participants used long-lasting insecticidal nets. Due to limited supply, participants were often unable to use mosquito repellent and coils when necessary. Because participants were unable to receive timely diagnosis and appropriate treatment for malaria in their camps, they commonly practiced self-treatment using antibiotics, painkillers, and/or traditional medicines. They only go to a healthcare facility through their supervisor if their conditions worsen.ConclusionsThe prevalence of symptomatic and asymptomatic malaria was conspicuous among military in forest areas. Many participants believed that malaria is transmitted not only by mosquito bites but also from drinking stream water. Preventive equipment was often insufficient. Self-treatment was practiced before referring to healthcare facility. To further prevent military from contracting malaria, the National Malaria Control Program and military body should provide adequate and suitable health education, protective equipment, and on-site malaria case management.
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34. Six Nearly Complete Genome Segments of a Novel Reovirus Identified in Laotian Batflies
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Paul T. Brey, Khamsing Vongphayloth, Marc Grandadam, Jeffrey C. Hertz, Bounsavane Douangboubpha, Marc Eloit, Ian W. Sutherland, Sarah Temmam, Thomas Bigot, Brey, Paul, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Naval Medical Research Unit Two [Singapore] (NAMRU-2), Naval Medical Research, Naval Air Station Jacksonville (NAS Jacksonville), United States Navy - U.S. Navy (USA), National University of Laos, Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), École nationale vétérinaire - Alfort (ENVA), This work was supported by the United States Naval Medical Research Unit Two, work unit number D1425, in support of the Armed Forces Health Surveillance Branch–Global Emerging Infections Surveillance and Response System (AFHSB-GEIS), by the Institute Pasteur du Laos, by Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' grant ANR-10-LABX-62-IBEID, and by the Direction Internationale de l'Institut Pasteur., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), and École nationale vétérinaire d'Alfort (ENVA)
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0303 health sciences ,viruses ,Genome Sequences ,030302 biochemistry & molecular biology ,virus diseases ,Computational biology ,biochemical phenomena, metabolism, and nutrition ,Biology ,Genome ,3. Good health ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,03 medical and health sciences ,Immunology and Microbiology (miscellaneous) ,Genetics ,[SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Molecular Biology ,030304 developmental biology - Abstract
As part of the characterization of viral communities of Laotian batflies, we report here the sequencing of six nearly complete genome segments of a novel reovirus identified in Laotian batflies that is distantly related to reoviruses recently reported in various Diptera species.
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35. Insights into the host range, genetic diversity, and geographical distribution of jingmenviruses
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Paul T. Brey, Veasna Duong, Vibol Hul, Stéphane Petres, Philippe Pérot, Delphine Chrétien, Evelyne Dufour, Valérie Pinarello, Muriel Vayssier-Taussat, Sarah Temmam, Emmanuel Albina, Elodie Devillers, Marine Dumarest, Thomas Bigot, Marc Grandadam, Thavry Hoem, Virginie Pommelet, Irène Loiseau, Jeffrey C. Hertz, Sara Moutailler, Khamsing Vongphayloth, Philippe Dussart, Julien Cappelle, Marc Eloit, Mathilde Gondard, Découverte de Pathogènes - Pathogen Discovery, Biologie des Infections - Biology of Infection, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Plateforme technologique Production et purification de protéines recombinantes – Production and Purification of Recombinant Proteins Technological Platform (PPR), Institut Pasteur du Cambodge, US Naval Medical Research Unit n°2, Unité Mixte de Recherche d'Épidémiologie des maladies Animales et zoonotiques (UMR EPIA), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), École nationale vétérinaire d'Alfort (ENVA), Université Paris-Est (UPE), This work was supported by Laboratoire d’Excellence (Integrative Biology of Emerging Infectious Diseases, grant no. ANR-10-LABX-62-IBEID) and by the Direction Internationale de l'Institut Pasteur., We thank Jacques Bellalou and his team at the Production and Purification of Recombinant Proteins Technological Platform of Institut Pasteur for their help in expression of recombinant proteins, Yves Jacob and Mélanie Dos Santos for their technical assistance with the luminometer, the staff members at the ICAReB Platform of Institut Pasteur for access to human control sera, and all the people involved in the sampling of ticks, bats, and cattle in France, French Antilles, Lao PDR, and Cambodia., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Eloit, Marc, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Plateforme technologique Production et purification de protéines recombinantes – Production and Purification of Recombinant Proteins Technological Platform, Institut Pasteur [Paris], Naval Medical Research Unit Two [Cambodge] (NAMRU-2), Naval Medical Research Center [Silver Spring, USA] (NMRC), Épidémiologie des Maladies Animales et Zoonotiques - UMR 346 (EPIA), ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, École nationale vétérinaire - Alfort (ENVA), and École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé
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0301 basic medicine ,lcsh:QR1-502 ,jingmenvirus ,Global Health ,L73 - Maladies des animaux ,lips ,lcsh:Microbiology ,Ticks ,Blood serum ,Chiroptera ,Tick-borne disease ,biology ,Microbiology and Parasitology ,Amblyomma testudinarium ,Jingmenvirus ,LIPS ,emergence ,evolution ,QR1-502 ,Microbiologie et Parasitologie ,3. Good health ,Phylogeography ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Rhipicephalus microplus ,Martinique ,L72 - Organismes nuisibles des animaux ,Research Article ,Autre (Sciences du Vivant) ,Ixodes ricinus ,Distribution géographique ,030106 microbiology ,Zoology ,Ecological and Evolutionary Science ,Relation hôte pathogène ,Tick ,Microbiology ,Arbovirus ,Host Specificity ,Virus des animaux ,03 medical and health sciences ,Variation génétique ,parasitic diseases ,Filoviridae Infections ,medicine ,Animals ,Humans ,Molecular Biology ,Maladie transmissible par tiques ,Flaviviridae ,Genetic Variation ,biology.organism_classification ,medicine.disease ,030104 developmental biology ,Cattle - Abstract
Several arboviruses emerging as new pathogens for humans and domestic animals have recently raised public health concern and increased interest in the study of their host range and in detection of spillover events. Recently, a new group of segmented Flaviviridae-related viruses, the Jingmenviruses, has been identified worldwide in many invertebrate and vertebrate hosts, pointing out the issue of whether they belong to the arbovirus group. The study presented here combined whole-genome sequencing of three tick-borne Jingmenviruses and one bat-borne Jingmenvirus with comprehensive phylogenetic analyses and high-throughput serological screening of human and cattle populations exposed to these viruses to contribute to the knowledge of Jingmenvirus host range, geographical distribution, and mammalian exposure., Jingmenvirus is a recently identified group of segmented RNA viruses phylogenetically linked with unsegmented Flaviviridae viruses. Primarily identified in various tick genera originating in China, Jingmenvirus geographical distribution has rapidly expanded to cover Africa, South America, Caribbean, and Europe. The identification of Jingmen-related viruses in various mammals, including febrile humans, opens the possibility that Jingmenviruses may be novel tick-borne arboviruses. In this study, we aimed at increasing knowledge of the host range, genetic diversity, and geographical distribution of Jingmenviruses by reporting for the first time the identification of Jingmenviruses associated with Rhipicephalus microplus ticks originating in the French Antilles (Guadeloupe and Martinique islands), with Amblyomma testudinarium ticks in Lao PDR, and with Ixodes ricinus ticks in metropolitan France, and from urine of Pteropus lylei bats in Cambodia. Analyses of the relationships between the different Jingmenvirus genomes resulted in the identification of three main phylogenic subclades, each of them containing both tick-borne and mammal-borne strains, reinforcing the idea that Jingmenviruses may be considered as tick-borne arboviruses. Finally, we estimated the prevalence of Jingmenvirus-like infection using luciferase immunoprecipitation assay screening (LIPS) of asymptomatic humans and cattle highly exposed to tick bites. Among 70 French human, 153 Laotian human, and 200 Caribbean cattle sera tested, only one French human serum was found (slightly) positive, suggesting that the prevalence of Jingmenvirus human and cattle infections in these areas is probably low. IMPORTANCE Several arboviruses emerging as new pathogens for humans and domestic animals have recently raised public health concern and increased interest in the study of their host range and in detection of spillover events. Recently, a new group of segmented Flaviviridae-related viruses, the Jingmenviruses, has been identified worldwide in many invertebrate and vertebrate hosts, pointing out the issue of whether they belong to the arbovirus group. The study presented here combined whole-genome sequencing of three tick-borne Jingmenviruses and one bat-borne Jingmenvirus with comprehensive phylogenetic analyses and high-throughput serological screening of human and cattle populations exposed to these viruses to contribute to the knowledge of Jingmenvirus host range, geographical distribution, and mammalian exposure.
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36. Sampling adult populations of anopheles mosquitoes
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Alexandra Hiscox, Julie-Anne A. Tangena, Paul T. Brey, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Wageningen University and Research [Wageningen] (WUR), International Centre of Insect Physiology and Ecology (ICIPE), ICIPE, and This work was supported by the Yersin project, funded by the Michelin Corporate Foundation. AH was supported by a grant from the Innovative Vector Control Consortium.
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Peripheral net collection ,MESH: Malaria ,030231 tropical medicine ,Optimal deployment ,MESH: Disease Vectors ,Human-baited net trap ,Pyrethrum collection ,Resting shelter ,Trapping methods ,Biogents Suna trap ,MESH: Anopheles ,Trap (computing) ,03 medical and health sciences ,0302 clinical medicine ,Anopheles ,MESH: Animals ,030212 general & internal medicine ,Laboratory of Entomology ,Animal-baited net trap ,MESH: Mosquito Control ,MESH: Humans ,biology ,Sampling (statistics) ,MESH: Adult ,Animal landing catch ,CDC miniature light trap ,biology.organism_classification ,Laboratorium voor Entomologie ,3. Good health ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Fishery ,Vector (epidemiology) ,Mosquito traps ,Local environment ,Environmental science ,MESH: Mosquito Vectors ,Human landing catch ,Exit-entry trap - Abstract
International audience; For the control and elimination of malaria, information on the local vector dynamics is essential. This information provides guidance on appropriate and timely deployment of vector control tools and their subsequent success. The data on the dynamics of local mosquito populations can be collected using many different Anopheles sampling methods. Dependent on the objectives, resources, time, and local environment, different traps and methods can be chosen. This chapter describes the sampling of adult populations, focusing on the important preparatory stages and some of the widely used sampling methods. The trapping methods discussed in this chapter are the human landing catch, human-baited net trap, animal landing catch, animal-baited net trap, CDC miniature light trap, Biogents Suna trap, peripheral net collection, pyrethrum collection, exit/entry trap, and resting shelter. For optimal deployment in the field, a step-by-step description of the sampling methods is given.
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37. Hypoglycemic Toxins and Enteroviruses as Causes of Outbreaks of Acute Encephalitis-Like Syndrome in Children, Bac Giang Province, Northern Vietnam
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Marc Eloit, Meriadeg Ar Gouilh, Nghia D. Ngu, Johannes Sander, Charles Hebert, Nils Janzen, Paul T. Brey, Laura Tondeur, Juliette Paireau, Hien Tran Nguyen, Olivier Lortholary, Tuan Hai Nguyen, Arnaud Fontanet, Loan Phuong, Jean-Claude Manuguerra, Robert Barouki, Justine Cheval, Nga Thi Phan, National Institute of Hygiene and Epidemiology [Hanoi, Vietnam] (NIHE), Réseau International des Instituts Pasteur (RIIP), Groupe de Recherche sur l'Adaptation Microbienne (GRAM 2.0), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats (CIBU), Institut Pasteur [Paris] (IP), Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), PathoQuest, Régulation de l'expression génétique (REG), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre d'infectiologie Necker-Pasteur [CHU Necker], Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Génétique Moléculaire des Virus Respiratoires, Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Toxicité environnementale, cibles thérapeutiques, signalisation cellulaire (T3S - UMR_S 1124), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Hannover Medical School [Hannover] (MHH), Institut Pasteur du Laos, Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Découverte de Pathogènes - Pathogen Discovery, Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Le Gouil, Meriadeg, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Institut Pasteur [Paris], Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Institut Pasteur [Paris]-CHU Necker - Enfants Malades [AP-HP], Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris], and Environnement et Risques infectieux - Environment and Infectious Risks (ERI)
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0301 basic medicine ,Male ,Epidemiology ,encephalitis ,Physiology ,lcsh:Medicine ,medicine.disease_cause ,[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy ,0302 clinical medicine ,Cerebrospinal fluid ,methylenecyclopropylglycine ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,litchi ,Acute Febrile Encephalopathy ,Child ,Enterovirus ,[SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases ,[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases ,[SDV.TOX.ECO] Life Sciences [q-bio]/Toxicology/Ecotoxicology ,biology ,acute encephalitis syndrome ,Bac Giang Province ,3. Good health ,Infectious Diseases ,Vietnam ,Child, Preschool ,outbreaks ,litchi cultivation ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Female ,Seasons ,meningitis/encephalitis ,[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology ,Encephalitis ,Microbiology (medical) ,[SDE.MCG]Environmental Sciences/Global Changes ,030231 tropical medicine ,Aspartate transaminase ,Hypoglycemia ,hypoglycins ,enteroviruses ,hypoglycemic toxins ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,children ,[SDV.BID.SPT] Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy ,medicine ,Enterovirus Infections ,Humans ,lcsh:RC109-216 ,viruses ,Retrospective Studies ,Hypoglycemic Toxins and Enteroviruses as Causes of Outbreaks of Acute Encephalitis-Like Syndrome in Children, Bac Giang Province, Northern Vietnam ,business.industry ,Viral encephalitis ,Research ,lcsh:R ,Outbreak ,Infant ,medicine.disease ,acute encephalitis-like syndrome ,[SDE.MCG] Environmental Sciences/Global Changes ,030104 developmental biology ,hypoglycemia ,[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie ,biology.protein ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,business - Abstract
International audience; We investigated the cause of seasonal outbreaks of pediatric acute encephalitis-like syndrome associated with litchi harvests (May-July) in northern Vietnam since 2008. Nineteen cerebrospinal fluid samples were positive for human enterovirus B, and 8 blood samples were positive for hypoglycemic toxins present in litchi fruits. Patients who were positive for hypoglycemic toxins had shorter median times between disease onset and admission, more reports of seizures, more reports of hypoglycemia (glucose level
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- 2018
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38. Contemporary status of insecticide resistance in the major Aedes vectors of arboviruses infecting humans
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Catherine L. Moyes, Lee Ching Ng, Sin Ying Koou, Isabelle Dusfour, Ademir Jesus Martins, João Pinto, Kamaraju Raghavendra, John Vontas, David Weetman, Jean-Philippe David, Vincent Corbel, Big Data Institute, University of Oxford, Institute of Molecular Biology and Biotechnology (IMBB-FORTH), Foundation for Research and Technology - Hellas (FORTH), Agricultural University of Athens, Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP), Environment Health Institute [Singapore], National Environment Agency [Singapore] (NEA), Entomologie médicale, Laboratoire de Parasitologie [Cayenne, Guyane française], Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), National Institute of Malaria Research [New Dehli, Inde] (NIMR), Indian Council of Medical Research [New Dehli] (ICMR), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Laboratoire d'Ecologie Alpine (LECA ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Liverpool School of Tropical Medicine (LSTM), The preparation of this review was funded by an award from the World Health Organization's Special Programme for Research and Training in Tropical Diseases (http://www.who.int/tdr/) to VC, JPD, and the WIN network. The bioassay data extraction was funded by Wellcome Trust (https://wellcome.ac.uk/) grant 108440/Z/15/Z awarded to CLM., We thank Hilary Ranson for providing the complete datasets from her 2010 review. We also thank Maria de Lourdes Macoris, Laboratório de Entomologia Aplicada, Sucen, São Paulo, for compiling an extensive dataset for Brazil, and Poonam Sharma Velamuri and Elamathi Natarajan for their work on the data for India. We thank the following groups and individuals for contributing unpublished datasets: Gonçalo Seixas and Carla Alexandra Sousa, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Beniamino Caputo and Alessandra della Torre, Istituto Pasteur-Fondazione Cenci-Bolognetti, Dipartimento di Sanità Pubblica e Malattie Infettive, Università 'Sapienza', Rome, Italy, Sébastien Marcombe and Paul Brey, Institut Pasteur du Laos, Vientiane, Lao People’s Democratic Republic Waraporn Juntarajumnong, Kasetsart University, Bangkok, Thailand, Ferdinand V. Salazar, Department of Medical Entomology, Research Institute of Tropical Medicine, Department of Health, Philippines, Laboratório de Entomologia Aplicada (LEnA), Sucen, Marília, SP/ Brazil., University of Oxford [Oxford], and Fundação Oswaldo Cruz (FIOCRUZ)
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Enzyme Metabolism ,Gene Identification and Analysis ,MESH: Insect Vectors/genetics ,Review ,MESH: Africa ,Dengue virus ,Biochemistry ,Zika virus ,0302 clinical medicine ,MESH: Insecticides/classification ,MESH: Animals ,Chikungunya ,Enzyme Chemistry ,MESH: Larva/drug effects ,MESH: Aedes/genetics ,Organic Compounds ,MESH: Asia ,Yellow fever ,MESH: Arboviruses/pathogenicity ,3. Good health ,Physical Sciences ,Inactivation, Metabolic ,Biological Assay ,Agrochemicals ,Ethers ,lcsh:RC955-962 ,MESH: Insect Control/methods ,Aedes Aegypti ,MESH: Biological Assay ,03 medical and health sciences ,qx_600 ,Genetics ,MESH: Aedes/drug effects ,Humans ,Mutation Detection ,MESH: Humans ,Organisms ,Chemical Compounds ,Public Health, Environmental and Occupational Health ,Biology and Life Sciences ,Correction ,lcsh:RA1-1270 ,MESH: Insecticide Resistance/genetics ,medicine.disease ,Invertebrates ,Virology ,Geographic Distribution ,Insect Vectors ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Species Interactions ,030104 developmental biology ,MESH: Arboviruses/classification ,Infectious disease (medical specialty) ,Africa ,MESH: Inactivation, Metabolic ,Americas ,0301 basic medicine ,Insecticides ,MESH: Insect Vectors/virology ,viruses ,Population Dynamics ,Disease Vectors ,medicine.disease_cause ,Mosquitoes ,Insecticide Resistance ,Aedes ,Genotype ,Medicine and Health Sciences ,MESH: Insecticides/pharmacology ,lcsh:Public aspects of medicine ,Agriculture ,Organophosphates ,MESH: Insect Vectors/drug effects ,Insects ,Chemistry ,Infectious Diseases ,Larva ,lcsh:Arctic medicine. Tropical medicine ,Asia ,Arthropoda ,030231 tropical medicine ,wc_524 ,Aedes aegypti ,Biology ,Insect Control ,Virus ,medicine ,qx_525 ,MESH: Americas ,Animals ,Population Biology ,MESH: Aedes/virology ,Organic Chemistry ,biology.organism_classification ,qx_650 ,Enzymology ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Arboviruses - Abstract
International audience; Both Aedes aegytpi and Ae. albopictus are major vectors of 5 important arboviruses (namely chikungunya virus, dengue virus, Rift Valley fever virus, yellow fever virus, and Zika virus), making these mosquitoes an important factor in the worldwide burden of infectious disease. Vector control using insecticides coupled with larval source reduction is critical to control the transmission of these viruses to humans but is threatened by the emergence of insecticide resistance. Here, we review the available evidence for the geographical distribution of insecticide resistance in these 2 major vectors worldwide and map the data collated for the 4 main classes of neurotoxic insecticide (carbamates, organochlorines, organophosphates, and pyrethroids). Emerging resistance to all 4 of these insecticide classes has been detected in the Americas, Africa, and Asia. Target-site mutations and increased insecticide detoxification have both been linked to resistance in Ae. aegypti and Ae. albopictus but more work is required to further elucidate metabolic mechanisms and develop robust diagnostic assays. Geographical distributions are provided for the mechanisms that have been shown to be important to date. Estimating insecticide resistance in unsampled locations is hampered by a lack of standardisation in the diagnostic tools used and by a lack of data in a number of regions for both resistance phenotypes and genotypes. The need for increased sampling using standard methods is critical to tackle the issue of emerging insecticide resistance threatening human health. Specifically, diagnostic doses and well-characterised susceptible strains are needed for the full range of insecticides used to control Ae. aegypti and Ae. albopictus to standardise measurement of the resistant phenotype, and calibrated diagnostic assays are needed for the major mechanisms of resistance.
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- 2017
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39. Modeling spatial variation in risk of presence and insecticide resistance for malaria vectors in Laos
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Vincent Corbel, Julie Laforet, Sébastien Marcombe, Marc Souris, Paul T. Brey, Hans J. Overgaard, Emergence des Pathologies Virales (EPV), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Université de Toulouse le Mirail, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM), Génétique et évolution des maladies infectieuses (GEMI), and Mivegec, HAL
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0301 basic medicine ,Insecticides ,Geographic information system ,Rain ,lcsh:Medicine ,Social Sciences ,Disease Vectors ,Mosquitoes ,Geographical Locations ,Insecticide Resistance ,0302 clinical medicine ,Risk Factors ,Medicine and Health Sciences ,Insect vectors ,lcsh:Science ,2. Zero hunger ,Likelihood Functions ,Multidisciplinary ,biology ,Geography ,Anopheles ,Temperature ,Agriculture ,3. Good health ,Insects ,Infectious Diseases ,Laos ,Agrochemicals ,[SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,Research Article ,medicine.medical_specialty ,Asia ,Arthropoda ,030231 tropical medicine ,Human Geography ,03 medical and health sciences ,Population Metrics ,Environmental health ,parasitic diseases ,medicine ,Parasitic Diseases ,Animals ,Humans ,[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,Pesticides ,Probability ,Resistance (ecology) ,Population Biology ,business.industry ,Public health ,lcsh:R ,Organisms ,Biology and Life Sciences ,Pesticide ,Models, Theoretical ,medicine.disease ,biology.organism_classification ,Tropical Diseases ,Invertebrates ,Malaria ,Species Interactions ,030104 developmental biology ,[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie ,People and Places ,Land use ,Earth Sciences ,Population density ,Spatial variability ,lcsh:Q ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Pest Control ,business - Abstract
International audience; Climatic, sociological and environmental conditions are known to affect the spatial distribution of malaria vectors and disease transmission. Intensive use of insecticides in the agricultural and public health sectors exerts a strong selective pressure on resistance genes in malaria vectors. Spatio-temporal models of favorable conditions for Anopheles species' presence were developed to estimate the probability of presence of malaria vectors and insecticide resistance in Lao PDR. These models were based on environmental and meteorological conditions, and demographic factors. GIS software was used to build and manage a spatial database with data collected from various geographic information providers. GIS was also used to build and run the models. Results showed that potential insecticide use and therefore the probability of resistance to insecticide is greater in the southwestern part of the country, specifically in Champasack province and where malaria incidence is already known to be high. These findings can help national authorities to implement targeted and effective vector control strategies for malaria prevention and elimination among populations most at risk. Results can also be used to focus the insecticide resistance surveillance in Anopheles mosquito populations in more restricted area, reducing the area of surveys, and making the implementation of surveillance system for Anopheles mosquito insecticide resistance possible.
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- 2017
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40. Insecticide resistance status of malaria vectors in Lao PDR
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Boutsady Somphong, Paul T. Brey, Sébastien Marcombe, Vincent Corbel, Santi Maithaviphet, Nothasin Phommavan, Simone Nambanya, Julie Bobichon, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), and Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])
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0301 basic medicine ,Veterinary medicine ,Insecticides ,Enzyme Metabolism ,lcsh:Medicine ,Disease Vectors ,Mosquitoes ,Biochemistry ,Sodium Channels ,Insecticide Resistance ,chemistry.chemical_compound ,0302 clinical medicine ,Pyrethrins ,Medicine and Health Sciences ,Malaria vector ,lcsh:Science ,Enzyme Chemistry ,education.field_of_study ,Multidisciplinary ,Pyrethroid ,Anopheles ,Agriculture ,3. Good health ,Insects ,Infectious Diseases ,Laos ,Agrochemicals ,medicine.drug ,Research Article ,Arthropoda ,Infectious Disease Control ,Death Rates ,030231 tropical medicine ,Population ,Biology ,Ddt ,03 medical and health sciences ,Nitriles ,parasitic diseases ,medicine ,Parasitic Diseases ,Animals ,education ,Permethrin ,Demography ,lcsh:R ,Organisms ,Biology and Life Sciences ,Knockdown resistance ,medicine.disease ,biology.organism_classification ,Tropical Diseases ,Invertebrates ,Insect Vectors ,Malaria ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Species Interactions ,030104 developmental biology ,Deltamethrin ,chemistry ,Mutation ,People and Places ,Enzymology ,lcsh:Q ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
International audience; Knowledge on insecticide resistance in Anopheles species is a basic requirement to guide malaria vector control programs. In Lao PDR, vector control relies on insecticide residual spraying (IRS) and impregnated bed-nets (ITNs) with the use of pyrethroids. Here, the susceptibility of Anopheles species, including several malaria vectors (An. maculatus and An. minimus), to various insecticides was investigated in ten provinces of Lao PDR through a north-south transect. Bioassays were performed on field caught female mosquitoes using the standard WHO susceptibility tests with DDT (4%), deltamethrin (0.05%) and permethrin (0.75%). In addition, the DIIS6 region of the para-type sodium channel gene was amplified and sequenced to identify knockdown resistance mutations (kdr). Resistance to DDT and permethrin was detected in suspected malaria vectors, such as An. nivipes and An. philippi-nensis in Lao PDR. Resistance to the formerly used DDT was found in a population of An. maculatus s.l. from Luang Prabang province. No resistance to pyrethroids was found in primary vectors, indicating that these insecticides are still adequate for malaria vector control. However, high resistance levels to pyrethroids was found in-vector species and reduced susceptibility to permethrin in An. minimus and An. maculatus was reported in specific localities which raises concerns for pyrethroid-based control in the future. No kdr mutation was found in any of the resistant populations tested hence suggesting a probable role detoxifica-tion enzymes in resistance. This study highlights the necessity to continue the monitoring of insecticide susceptibility to early detect potential occurrence and/or migration of insecticide resistance in malaria vectors in Lao PDR.
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- 2017
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41. A Worldwide Map of Plasmodium falciparum K13-Propeller Polymorphisms
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Benoit Witkowski, Hans-Peter Fuehrer, Garib Das Thakur, Céline Barnadas, Djibrine Djalle, Michael Ramharter, Mindy Leelawong, Wasif Ali-Khan, Harald Noedl, Bouasy Hongvanthong, Mohammad Shafiul-Alam, Hypolite Muhindo-Mavoko, Abdillahi Mohamed Hassan, Judith Straimer, Nimol Khim, Kigbafori D. Silué, Kaknika Loch, Barbara H. Stokes, Maria Dorina Bustos, Laura Berne, Dylan R. Pillai, Ayola A. Adegnika, Lin Hua Tang, Rotha Eam, Saorin Kim, Alioune Dieye, Mei Li, Carole E. Eboumbou-Moukoko, Lydie Canier, Marian Warsame, Didier Menard, David A. Fidock, Yap Boum, Lyndes Wini, Abdiqani Sheikh-Omar, Patrick Tshibangu-Wa-Tshibangu, Maman Laminou Ibrahim, Mohammad Jahirul-Karim, Malen Ken, Monique A. Dorkenoo, Sócrates Herrera, Odile Mercereau-Puijalon, Lise Musset, Valentine Duru, Eric Legrand, Maniphone Khanthavong, Pascal Ringwald, Bruno Pradines, Sandrine Houzé, Rachida Tahar, Olukemi K. Amodu, Johann Beghain, Sandie Menard, Liwang Cui, Colin J. Sutherland, Jun Hu Chen, Kesara Na-Bangchang, Khin Lin, Michael Nambozi, Rithea Leang, Jean Christophe Barale, Milijaona Randrianarivelojosia, Marcus V. G. Lacerda, Sophy Chy, Frédéric Ariey, Jean-Bosco Ouédraogo, Isabelle Morlais, Maria de Fátima Ferreira-da-Cruz, Lubin Jiang, Christophe Rogier, Jun Cao, Peter G. Kremsner, Bui Quang-Phuc, Inès Vigan-Womas, Din Syafruddin, Jetsumon Sattabongkot, Shigeyuki Kano, Abebe A. Fola, Louis Collet, Karamoko Niaré, Thierry Fandeur, Sedigheh Zakeri, Sodiomon B. Sirima, Antoine Berry, Jean Baptiste Mazarati, Fe Espino, Ghulam Rahim-Awab, Chanra Khean, Offianan Andre Toure, Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Génétique et Génomique des Insectes vecteurs, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Eberhard Karls Universität Tübingen = Eberhard Karls University of Tuebingen, Centre de Recherche Médicale de Lambaréné, Albert Schweitzer, Leiden University Medical Center (LUMC), Universiteit Leiden, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), University of Ibadan, Nangarhar University, Mahidol University [Bangkok], The Walter and Eliza Hall Institute of Medical Research (WEHI), University of Melbourne, Papua New Guinea Institute for Medical Research (PNGIMR), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Mbarara University of Science and Technology [Mbarara] (MUST), Epicentre Ouganda [Mbarara] [Médecins Sans Frontières], Epicentre [Paris] [Médecins Sans Frontières], World Health Organization (WHO), country office for Thailand, Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), JiangSu University, National Institute of Parasitic Diseases, Center for Disease Control, China, Centre Hospitalier de Mayotte, Pennsylvania State University (Penn State), Penn State System, Epidemiology and Disease Control division (EDCD), Ministry of Public Health [Nepal], Institut Pasteur de Dakar, Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD), Institut Pasteur de Bangui, Université de Lomé [Togo], Université de Douala, Centre Pasteur du Cameroun, Research Institute for Tropical Medicine, Centre International de Recherches Médicales de Franceville (CIRMF), Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), University of Gondar, Institute of Parasitology [Vienna], University of Veterinary Medicine, Vienna, World Health Organisation (WHO), country office for Somalia, Caucaseco scientific research center = Centro de Investigación Científica Caucaseco, National Center for Malariology, Parasitology and Entomology, Ministry of Health [Mozambique], Hôpital Bichat - Claude Bernard, Mère et enfant en milieu tropical : pathogènes, système de santé et transition épidémiologique (MERIT - UMR_D 216), Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5), Centre de Recherche Médicale et Sanitaire (Niamey, Niger) (CERMES), Directorate General of Health Services (DGHS), Institut Pasteur de Shanghai, Académie des Sciences de Chine - Chinese Academy of Sciences (IPS-CAS), National Center for Global Health and Medicine [Japan] (NCGM), Institut Pasteur du Laos, Instituto Leônidas e Maria Deane - Fiocruz Amazônia [Manaus, Brésil] (ILMD), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Fundação de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD), National Center for Malaria Control, Parasitology and Entomology, Ministry of Health of Cambodgia, Henry M. Jackson Foundation for the Advancement of Military Medicine (HJM), U.S. Naval Medical Research, The Department of Medical Research (Upper Myanmar), Rwanda Biomedical Center (RBC), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement [Yaoundé, Cameroun] (IRD [Cameroun]), Institut de Recherche pour le Développement (IRD), University of Kinshasa (UNIKIN), University of Antwerp (UA), Institut Pasteur de la Guyane, Thammasat University (TU), Tropical Diseases Research Center (TDRC), Université de Bamako, Medizinische Universität Wien = Medical University of Vienna, Institut de Recherche en Sciences de la Santé (IRSS) / Centre Muraz, Medicines for Malaria Venture (MMV), Université de Genève = University of Geneva (UNIGE), Global Malaria Programme, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre National de Référence du Paludisme, Institut Pasteur de Madagascar, Ministry of Health and Human Services [Somalia], Centre Suisse de Recherches Scientifiques en Cote d'Ivoire [Abidjan] (CSRS-CI), Université Félix Houphouët-Boigny (UFHB), Groupe de recherche action en santé (GRAS), London School of Hygiene and Tropical Medicine (LSHTM), University of Hasanuddin, Eijkman Institute for Molecular Biology [Jakarta], Institut Pasteur de Côte d'Ivoire, Ministry of Health and Medical Services, Institut Pasteur d'Iran, Centre National de Référence du Paludisme [Cayenne, Guyane française] (CNR - laboratoire associé), Microbiologie structurale - Structural Microbiology (Microb. Struc. (UMR_3528 / U-Pasteur_5)), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Columbia University Irving Medical Center (CUIMC), Institut de Recherche Biomédicale des Armées [Antenne Marseille] (IRBA), AP-HP - Hôpital Cochin Broca Hôtel Dieu [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Descartes - Paris 5 (UPD5), Supported by the Institut Pasteur Paris, Institut Pasteur International Division, Institut Pasteur Cambodia, and the World Health Organization, by a grant (ANR-10-LABX-62-IBEID) from the French Government Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases', a grant from Natixis Banques, a grant (R01I109023, to Dr. Fidock) from the National Institutes of Health, grants from the Fiocruz Fundação Oswaldo Cruz, Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro, Fundação de Amparo à Pesquisa do Estado do Amazonas, the Brazilian National Council for Scientific and Technological Development, the Agence Nationale de la Recherche (13-BSV3-0018-01 and11-BSV7-009-01), the Austrian Federal Ministry of Science, Research, and Econo-my, the Calgary Laboratory Services, the Centre International de Recherches Médicales de Franceville, the European and Developing Countries Clinical Trials Partnership (CT-2004-31070-001), the Drugs for Neglected Diseases Initiative, the Else Kroener Fresenius Stiftung, the Holger Poehlmann Stiftung, the European Community African–European Research Initiative 'IDEA' (HEALTH-F3-2009-241642), the Fonds Wetenschappelijk Onderzoek, the Vlaamse Interuniversitaire Raad–Universitaire Ontwikkelingssamenwerking, the Belgian Technical Cooperation in Democratic Republic of Congo, the European Community Seventh Framework Program (FP7/2007-2013, 242095, and 223601), the European Commission (REGPOT-CT-2011-285837-STRONGER), the Ministère de la Santé Publique du Niger (Laboratoire National de Référence Résistance aux Antipaludiques), the Foundation of National Science and Technology Major Program (2012ZX10004-220), the French Ministry of Health (Institut National de Veille Sanitaire), the Global Fund to Fight AIDS, Tuberculosis and Malaria, the 5% Initiative program (French Ministry of Foreign Affairs, France Expertise Internationale, 12INI109), the Institut Pasteur de Madagascar, the Government of the Philippines, the Institut de Recherche pour le Développement, the Foundation des Treilles, the Délégation Générale pour l’Armement (PDH-2-NRBC-4-B1-402), the Institut Pasteur de Bangui, the International Society for Health Research and Training, the Malaria Research Initiative Bandarban, Vienna, International Centre for Diarrhoeal Disease Research, Bangladesh, the Médecins sans Frontières (Centre Opérationnel Paris, France), Medicines for Malaria Venture, the National Research Council of Thailand, the Thammasat University, the National Natural Science Foundation of China (81271870, 81361120405, and 81271863), the Natural Science Foundation of Jiangsu Province (BK20130114 and BK20150001), the Jiangsu Science and Technology Department (BM2015024), the National Institutes of Health (R01 AI11646601, AI109023, and ICEMR U19AI089702, U19AI089672), the Pasteur Institute of Iran, the Malaria Division of the Iranian Center for Diseases Management and Control, Public Health England (Malaria Reference Service Contract), the Government of Rwanda, the U.S. Department of Defense Armed Forces Health Surveillance Center, Global Emerging Infections Surveillance and Response System (P0463-14-N6), the Fogarty International Center of the National Institutes of Health training (D43 TW007393), the Mahidol-Oxford Research Unit, the Government of Japan (Science and Technology Agency, Agency for Medical Research and Development, Japan International Cooperation Agency, and Science and Technology Research Partnership for Sustainable Development), and the President’s Malaria Initiative of the U.S. Agency for International Development., The KARMA Consortium, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-13-BSV3-0018,MALARTRES,Résistance de Plasmodium aux antipaludiques de la famille des artémisinines(2013), ANR-13-BSV7-0009,NEBEDIV,Le rôle des ennemis naturels dans la diversité béta des arbres tropicaux(2013), European Project: CT-2004-31070-001,EDCCTP, European Project: HEALTH-F3-2009-24164,IDEA, European Project: FP7/2007-2013, 24209,FP7, European Project: FP7/2007-2013, 22360,FP7, European Project: 285837,EC:FP7:REGPOT,FP7-REGPOT-2011-1,STRONGER(2011), European Project: 242095,EC:FP7:HEALTH,FP7-HEALTH-2009-single-stage,EVIMALAR(2009), European Project: 223601,EC:FP7:HEALTH,FP7-HEALTH-2007-B,MALVECBLOK(2009), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), CHU Toulouse [Toulouse], Fundação Oswaldo Cruz (FIOCRUZ), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Genève (UNIGE), INSB-INSB-Centre National de la Recherche Scientifique (CNRS), Centre National de Référence du Paludisme [Cayenne, Guyane française] (CNR), Université Paris Diderot - Paris 7 (UPD7)-Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Eberhard Karls Universität Tübingen, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia., Centre International de Recherches Médicales de Franceville, Caucaseco scientific research center, Mère et enfant face aux infections tropicales (MERIT - UMR_D 216), National Center for Global Health and Medicine (NCGM), Instituto Leônidas e Maria Deane (ILMD), Centre de Physiopathologie Toulouse Purpan ex IFR 30 et IFR 150 (CPTP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement [Yaoundé], Centre Suisse de Recherches Scientifiques en Côte d'Ivoire, Eijkman Institute for Molecular Biology, Laboratoire de Parasitologie, Centre National de Référence du Paludisme - Région Antilles-Guyane, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-WHO Collaborating Center for Surveillance of Antimalarial Drug Resistance, Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010), Van geertruyden, Jean-Pierre, and KARMA Consortium
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0301 basic medicine ,Nonsynonymous substitution ,MESH: Sequence Analysis, DNA ,Endemic Diseases ,MESH: Asia, Southeastern ,Drug Resistance ,Protozoan Proteins ,Drug resistance ,MESH: Genotype ,Lactones ,0302 clinical medicine ,Genotype ,Artemisinin ,Malaria, Falciparum ,MESH: Protozoan Proteins ,Asia, Southeastern ,MESH: Plasmodium falciparum ,Genetics ,biology ,MESH: Malaria, Falciparum ,General Medicine ,Artemisinins ,MESH: China ,3. Good health ,MESH: Endemic Diseases ,MESH: Drug Resistance ,Algorithms ,MESH: Lactones ,medicine.drug ,China ,MESH: Mutation ,030231 tropical medicine ,030106 microbiology ,Plasmodium falciparum ,MESH: Algorithms ,03 medical and health sciences ,parasitic diseases ,MESH: Artemisinins ,MESH: Polymorphism, Genetic ,medicine ,Humans ,[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,Allele ,Polymorphism, Genetic ,MESH: Humans ,Haplotype ,Sequence Analysis, DNA ,medicine.disease ,biology.organism_classification ,Virology ,Mutation ,Human medicine ,Malaria - Abstract
Comment inK13-Propeller Mutations and Malaria Resistance, http://www.nejm.org/doi/full/10.1056/NEJMe1604520; International audience; BACKGROUND:Recent gains in reducing the global burden of malaria are threatened by the emergence of Plasmodium falciparum resistance to artemisinins. The discovery that mutations in portions of a P. falciparum gene encoding kelch (K13)-propeller domains are the major determinant of resistance has provided opportunities for monitoring such resistance on a global scale.METHODS:We analyzed the K13-propeller sequence polymorphism in 14,037 samples collected in 59 countries in which malaria is endemic. Most of the samples (84.5%) were obtained from patients who were treated at sentinel sites used for nationwide surveillance of antimalarial resistance. We evaluated the emergence and dissemination of mutations by haplotyping neighboring loci.RESULTS:We identified 108 nonsynonymous K13 mutations, which showed marked geographic disparity in their frequency and distribution. In Asia, 36.5% of the K13 mutations were distributed within two areas--one in Cambodia, Vietnam, and Laos and the other in western Thailand, Myanmar, and China--with no overlap. In Africa, we observed a broad array of rare nonsynonymous mutations that were not associated with delayed parasite clearance. The gene-edited Dd2 transgenic line with the A578S mutation, which expresses the most frequently observed African allele, was found to be susceptible to artemisinin in vitro on a ring-stage survival assay.CONCLUSIONS:No evidence of artemisinin resistance was found outside Southeast Asia and China, where resistance-associated K13 mutations were confined. The common African A578S allele was not associated with clinical or in vitro resistance to artemisinin, and many African mutations appear to be neutral. (Funded by Institut Pasteur Paris and others.).
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- 2016
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42. Chikungunya Virus and the Mosquito Vector Aedes aegypti in New Caledonia (South Pacific Region)
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Marc Grandadam, Valérie Caro, Jean-Michel Thiberge, Anna-Bella Failloux, Marie Vazeille, Ann-Claire Gourinat, Myrielle Dupont-Rouzeyrol, Laurent Guillaumot, Noémie Baroux, Eric D'Ortenzio, Laboratoire d'Epidémiologie Moléculaire, Institut Pasteur de Nouvelle-Calédonie, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8), Institut Pasteur [Paris], Réseau International des Instituts Pasteur (RIIP), Génétique Moléculaire des Bunyavirus, Institut Pasteur du Laos, Centre national de Référence et Centre Collaborateur de l'OMS Arbovirus (CNR-CCOMS), Institut Pasteur (New Caledonia and Paris), the Government of New Caledonia, and the French Institut de Veille Sanitaire (InVS, SaintMaurice, France), and Institut Pasteur [Paris] (IP)
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Insecticides ,chikungunya ,medicine.disease_cause ,Disease Outbreaks ,Insecticide Resistance ,Aedes aegypti ,0302 clinical medicine ,Aedes ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Pyrethrins ,Chikungunya ,Phylogeny ,Travel ,vector competence ,0303 health sciences ,biology ,virus diseases ,Infectious Diseases ,arboviruses ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Female ,Chikungunya virus ,Aedes albopictus ,Genotype ,Molecular Sequence Data ,030231 tropical medicine ,Genome, Viral ,Microbiology ,Virus ,03 medical and health sciences ,Phylogenetics ,Virology ,medicine ,Animals ,Humans ,030304 developmental biology ,Base Sequence ,Alphavirus Infections ,Outbreak ,Sequence Analysis, DNA ,new caledonia ,biology.organism_classification ,Insect Vectors ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Amino Acid Substitution ,Vector (epidemiology) ,Mutation ,Chikungunya Fever - Abstract
International audience; Abstract Chikungunya virus (CHIKV) is transmitted to humans through the bite of Aedes mosquitoes. During the 2005-2006 epidemic that occurred in the Indian Ocean Islands, a viral strain harboring a substitution of an alanine to valine at position 226 (E1-A226V) of the E1 glycoprotein enhanced the transmissibility of CHIKV by Aedes albopictus. In March 2011, autochthonous transmission of CHIKV was reported in New Caledonia (NC), an island located in the southwest Pacific Ocean. This was the first report of local chikungunya (CHIK) transmission in this region of the world. Phylogenetic analysis based on the complete genome demonstrated that the CHIKV-NC strain isolated from the first autochthonous human case belongs to the Asian lineage. This is consistent with the Indonesian origin of CHIK cases previously imported and detected. Thus the CHIKV-NC does not present a valine substitution at position E1-226. In New Caledonia, the putative vector of CHIKV is Aedes aegypti, since no other potential vector has ever been described. For example, A. albopictus is not found in NC. Vector competence experiments showed that A. aegypti from New Caledonia was able to transmit, as early as 3 days post-infection, two CHIKV strains: CHIKV-NC belonging to the Asian lineage, and CHIKV-RE from Reunion Island harboring the E1-A226V mutation. Thus the extrinsic incubation period of both CHIKV strains in this vector species could be considered to be quite short. These results illustrate the threat of the spread of CHIKV in the South Pacific region. From February to June 2011 (the end of the alert), only 33 cases were detected. Implementation of drastic vector control measures and the occurrence of the cold season probably helped to limit the extent of the outbreak, but other factors may have also been involved and are discussed.
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- 2012
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43. Identification of a Novel Neuropathogenic Theiler's Murine Encephalomyelitis Virus
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Julian Buchrieser, Justine Cheval, Marc Eloit, Antoine Gessain, Simona Ozden, Meriadeg Ar-R. Gouilh, Laurence Fiette, Matthew R. Buckwalter, Paul T. Brey, Phan Thi Nga, Melissa E. Laird, Jean-Claude Manuguerra, Jean-François Bureau, Arnaud Fontanet, Matthew L. Albert, Immunobiologie des Cellules Dendritiques, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National d'Hygiène et d'Épidémiologie de Hanoi (NIHE), Réseau International des Instituts Pasteur (RIIP), Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats (CIBU), Institut Pasteur [Paris], Histopathologie humaine et Modèles animaux, Immunopathologie Virale, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Epidémiologie et Physiopathologie des Virus Oncogènes, Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8), Virologie UMR1161 (VIRO), Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA), Institut Pasteur du Laos, Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), Conservatoire National des Arts et Métiers [CNAM] (CNAM), This work was supported by grants from the Agence National de la Recherche (ANR) and an EURYI Award from the European Science Foundation (ESF)., Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Cellule d'Intervention Biologique d'Urgence (CIBU), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Epidémiologie des Maladies Emergentes, and Pasteur-Cnam risques infectieux et émergents (PACRI)
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MESH: Sequence Homology, Amino Acid ,Neuropathogenic ,viruses ,MESH: Amino Acid Sequence ,MESH: Spinal Cord ,Mice ,Demyelinating disease ,MESH: Capsid ,MESH: Animals ,MESH: Phylogeny ,Murine ,Phylogeny ,0303 health sciences ,biology ,030302 biochemistry & molecular biology ,Brain ,3. Good health ,Cardiovirus ,Spinal Cord ,Capsid ,Theilovirus ,Theiler’s ,MESH: Genome, Viral ,Molecular Sequence Data ,Immunology ,Genome, Viral ,Microbiology ,Virus ,MESH: Brain ,03 medical and health sciences ,MESH: Mice, Inbred C57BL ,Virology ,medicine ,Encephalitis Viruses ,Animals ,Amino Acid Sequence ,MESH: Mice ,Gene ,030304 developmental biology ,MESH: Molecular Sequence Data ,Sequence Homology, Amino Acid ,medicine.disease ,biology.organism_classification ,Mice, Inbred C57BL ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Viral Tropism ,MESH: Theilovirus ,Insect Science ,Tissue tropism ,Pathogenesis and Immunity ,MESH: Viral Tropism - Abstract
Theiler's murine encephalitis viruses (TMEV) are divided into two subgroups based on their neurovirulence. Persistent strains resemble Theiler's original viruses (referred to as the TO subgroup), which largely induce a subclinical polioencephalomyelitis during the acute phase of the disease and can persist in the spinal cord of susceptible animals, inducing a chronic demyelinating disease. In contrast, members of the neurovirulent subgroup cause an acute encephalitis characterized by the rapid onset of paralysis and death within days following intracranial inoculation. We report herein the characterization of a novel neurovirulent strain of TMEV, identified using pyrosequencing technology and referred to as NIHE. Complete coverage of the NIHE viral genome was obtained, and it shares
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- 2011
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44. Serological Investigations of Flavivirus Prevalence in Khammouane Province, Lao People's Democratic Republic, 2007–2008
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Thongchanh Sisouk, Nguyen Thi Thu Yen, C. Winter, Juliet E. Bryant, Paul T. Brey, Phengta Vongphrachanh, Richard Paul, Alexandra Hiscox, Samlane Phompida, Pany Sananikhom, Virasack Somoulay, Surinder Kaul, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), London School of Hygiene and Tropical Medicine (LSHTM), National Center for Laboratory and Epidemiology, Ministry of Health [Mozambique], Center for Malaria, Parasitology, and Entomology, Nam Theun 2 Power Company, Institut National d'Hygiène et d'Épidémiologie de Hanoi (NIHE), Institut Pasteur [Paris] (IP), Financial support: Contributions of J. E. Bryant were supported by Agence française de développement (AFD), SISEA/Pasteur Project. This project was supported by the Fondation EDF Diversiterre., and Institut Pasteur [Paris]
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Culex vishnui ,Male ,Veterinary medicine ,viruses ,MESH: Dengue ,Antibodies, Viral ,Dengue fever ,Dengue ,0302 clinical medicine ,MESH: Aged, 80 and over ,Seroepidemiologic Studies ,MESH: Child ,MESH: Flavivirus ,MESH: Animals ,030212 general & internal medicine ,MESH: Encephalitis, Japanese ,Child ,MESH: Aged ,Aged, 80 and over ,education.field_of_study ,MESH: Middle Aged ,biology ,virus diseases ,Articles ,Middle Aged ,MESH: Infant ,3. Good health ,Flavivirus ,MESH: Cattle ,Infectious Diseases ,MESH: Hemagglutination Inhibition Tests ,Laos ,Child, Preschool ,Female ,Adult ,Aedes albopictus ,Adolescent ,030231 tropical medicine ,Population ,Short Report ,MESH: Insect Vectors ,03 medical and health sciences ,Flaviviridae ,MESH: Cross-Sectional Studies ,Virology ,medicine ,Seroprevalence ,Animals ,Humans ,education ,Encephalitis, Japanese ,Aged ,MESH: Adolescent ,MESH: Humans ,MESH: Seroepidemiologic Studies ,business.industry ,MESH: Child, Preschool ,Infant ,MESH: Adult ,Japanese encephalitis ,Hemagglutination Inhibition Tests ,medicine.disease ,biology.organism_classification ,MESH: Male ,Insect Vectors ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Cross-Sectional Studies ,Culicidae ,MESH: Laos ,Parasitology ,Cattle ,business ,MESH: Culicidae ,MESH: Female ,MESH: Antibodies, Viral - Abstract
International audience; A large-scale cross-sectional seroprevalence study of dengue (DEN) and Japanese encephalitis (JE) was conducted in Khammouane province, Lao PDR, as part of the initial baseline health impact assessment of the Nam Theun 2 hydroelectric dam construction project. Health surveys were performed between May 2007 and February 2008 with serum samples collected from healthy individuals involved in the resettlement program of 16 villages (total surveyed population 4,369). Hemagglutination inhibition assay using flavivirus antigens (DENV1, DENV3, and JEV) performed on 1,708 plasma specimens revealed 30.4% (519) cross-reactive positives, and 10% (172) and 1.3% (22) positives to JEV or DENV, respectively. Entomological surveys conducted during the rainy season of 2008 indicated the presence of competent flavivirus vectors (Culex vishnui group and Aedes albopictus), although Aedes aegypti was not found. Continued surveillance and investigation is warranted to assess the clinical disease burden of flaviviruses in this area that is undergoing rapid ecological and demographic change.
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- 2010
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45. Impact of mosquito bites on asexual parasite density and gametocyte prevalence in asymptomatic chronic Plasmodium falciparum infections and correlation with IgE and IgG titers
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Bradley S. Schneider, Ramatoulaye Lawaly, Ibrahima Dia, Fatoumata Diene Sarr, Laurence Marrama, Isabelle Casademont, Mawlouth Diallo, Anavaj Sakuntabhai, Paul T. Brey, Diawo Diallo, Richard Paul, Lassana Konate, Salah Mecheri, Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP), Département de Biologie Animale, Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD), Génétique fonctionnelle des Maladies infectieuses - Functional Genetics of Infectious Diseases, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur du Laos, Center of Excellence for Vector and Vector-Borne Diseases, Mahidol University [Bangkok], Réponses Précoces aux Parasites et Immunopathologie (USC 2010), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris], This work was supported by the Strategic Anopheles Horizontal Research Programme, Institut Pasteur., Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)
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Antibodies, Protozoan ,Immunoglobulin E ,Cohort Studies ,0302 clinical medicine ,MESH: Reverse Transcriptase Polymerase Chain Reaction ,Parasite hosting ,MESH: Animals ,Malaria, Falciparum ,MESH: Cohort Studies ,MESH: Plasmodium falciparum ,MESH: Immunoglobulin G ,0303 health sciences ,Host Response and Inflammation ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,MESH: Malaria, Falciparum ,MESH: Immunoglobulin E ,MESH: Gene Expression Regulation ,Senegal ,Infectious Diseases ,medicine.symptom ,030231 tropical medicine ,Immunology ,Plasmodium falciparum ,Microbiology ,Insect bites and stings ,Asymptomatic ,MESH: Gene Expression Profiling ,03 medical and health sciences ,Immune system ,MESH: Insect Bites and Stings ,Antigen ,MESH: Senegal ,parasitic diseases ,medicine ,Gametocyte ,MESH: Antibodies, Protozoan ,Animals ,Humans ,Family ,MESH: Family ,030304 developmental biology ,MESH: Humans ,MESH: Chronic Disease ,Gene Expression Profiling ,Insect Bites and Stings ,medicine.disease ,biology.organism_classification ,Virology ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Culicidae ,Gene Expression Regulation ,Immunoglobulin G ,Chronic Disease ,biology.protein ,Parasitology ,MESH: Culicidae - Abstract
An immunomodulatory role of arthropod saliva has been well documented, but evidence for an effect on Plasmodium sp. infectiousness remains controversial. Mosquito saliva may orient the immune response toward a Th2 profile, thereby priming a Th2 response against subsequent antigens, including Plasmodium . Orientation toward a Th1 versus a Th2 profile promotes IgG and IgE proliferation, respectively, where the former is crucial for the development of an efficient antiparasite immune response. Here we assessed the direct effect of mosquito bites on the density of Plasmodium falciparum asexual parasites and the prevalence of gametocytes in chronic, asymptomatic infections in a longitudinal cohort study of seasonal transmission. We additionally correlated these parasitological measures with IgE and IgG antiparasite and anti-salivary gland extract titers. The mosquito biting density was positively correlated with the asexual parasite density but not asexual parasite prevalence and was negatively correlated with gametocyte prevalence. Individual anti-salivary gland IgE titers were also negatively correlated with gametocyte carriage and were strongly positively correlated with antiparasite IgE titers, consistent with the hypothesis that mosquito bites predispose individuals to develop an IgE antiparasite response. We provide evidence that mosquito bites have an impact on asymptomatic infections and differentially so for the production of asexual and sexual parasites. An increased research focus on the immunological impact of mosquito bites during asymptomatic infections is warranted, to establish whether strategies targeting the immune response to saliva can reduce the duration of infection and the onward transmission of the parasite.
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- 2012
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46. Strategic success for hydropower in Laos
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Jean-Pierre Katz, Paul T. Brey, Richard Paul, Phasouk Khammanithong, Surinder Kaul, Michel Robino, Pany Sananikhom, Gilles Guerrier, Ruedi Luthi, Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Génétique fonctionnelle des Maladies infectieuses - Functional Genetics of Infectious Diseases, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Nam Theun Power Company Limited, Khammouane Provincial Health Service, and Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris]
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Energy-Generating Resources ,MESH: Food Supply ,030231 tropical medicine ,Fisheries ,Food Supply ,MESH: Rivers ,03 medical and health sciences ,0302 clinical medicine ,Electricity ,Rivers ,MESH: Fisheries ,Animals ,MESH: Animals ,MESH: Ecosystem ,Environmental planning ,Ecosystem ,Hydropower ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,0303 health sciences ,Multidisciplinary ,business.industry ,MESH: Energy-Generating Resources ,MESH: Electricity ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Geography ,business - Abstract
International audience
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- 2011
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47. Monitoring insecticide resistance of adult and larval Aedes aegypti (Diptera: Culicidae) in Phnom Penh, Cambodia
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Sebastien Boyer, Pierre-Olivier Maquart, Kalyan Chhuoy, Kimhuor Suor, Moeun Chhum, Kimly Heng, Sokkeang Leng, Didier Fontenille, Sebastien Marcombe, Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), Institut Pasteur du Laos, The research was funded by the Institut Pasteur du Cambodge, ZIKAlliance, which is funded by the H2020 program of the European Union (award no. 734548), the FSPI-SUPREV project (FSPI 2019-17), and a Calmette and Yersin post-doctoral grant., and European Project: 734548,ZIKAlliance(2016)
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Insecticides ,Mosquito Vectors ,MESH: Dengue ,Infectious and parasitic diseases ,RC109-216 ,MESH: Biological Assay ,Dengue ,Insecticide Resistance ,Aedes ,parasitic diseases ,Animals ,MESH: Insecticide Resistance ,MESH: Animals ,Research ,MESH: Cambodia ,fungi ,MESH: Aedes ,MESH: Insecticides ,Infectious Diseases ,Larva ,[SDE]Environmental Sciences ,Biological Assay ,MESH: Mosquito Vectors ,Parasitology ,Cambodia ,MESH: Larva - Abstract
Background Dengue fever is a major public health concern in Cambodia, with thousands of cases every year in urban, suburban and rural areas of the country. The main vector of dengue fever in Cambodia is Aedes aegypti. The organophosphate larvicide temephos and adulticides belonging to the pyrethroid family have been widely used for decades by public health authorities to fight dengue vectors, but resistance of Ae. aegypti to these insecticides has been previously described for Cambodia. Methods In order to adapt the vector control strategy presently used in Cambodia, we tested 14 adulticides belonging to the carbamate, organochlorine, organophosphate, and pyrethroid insecticide families and three larvicides [temephos, spinosad and Bacillus thuringiensis ser. israelensis (Bti)] belonging to three different insecticide families (organophosphates, spinosyns and entomopathogenic bacteria). The standard procedures used here to test the adults and larvae of an Ae. aegypti population from Phnom Penh followed World Health Organization guidelines. Results For adults, high mortality rates were observed with carbamate, organophosphate and organochlorine (with the exception of dichlorodiphenyltrichloroethane) insecticides (i.e. between 87.6 and 100%), while low mortality rates were observed with all of the tested pyrethroid insecticides (i.e. between 1 and 35%). For larvae, no resistance against Bti was detected [resistance ratio (RR90 90 Conclusions The results of this study indicate that (i) Bti should be considered a serious alternative to temephos for the control of Ae. aegypti larvae; and (ii) the carbamate adulticides propoxur and bendiocarb should be employed instead of the widely used pyrethroid insecticides for the control of adult Ae. aegypti on land under mosaic farming and crop rotation in Cambodia, as the insects were found to be resistant to the latter types of insecticide. Research focusing on insecticide resistance and innovative and effective vector control strategies should be undertaken as a public health priority in Cambodia. Graphical abstract
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48. Asymptomatic malaria infections and associated risk factors in malaria-eliminating settings of Nong District, Savannakhet Province, Lao People's Democratic Republic.
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Bouwe T, Nonaka D, Buchy P, Hansana P, Khamlome B, Chayvangmanh V, Ahissou NCA, Chindavongsa K, Pongvongsa T, Banouvong V, and Iwagami M
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Background: As the Lao People's Democratic Republic is nearing malaria elimination, asymptomatic malaria infections remain a challenge to address. Control measures focusing on symptomatic persons do not effectively work for asymptomatic infections which often go undetected by conventional diagnostic tools. It is therefore crucial to understand the burden of asymptomatic malaria for tailored interventions to eliminate the disease. This study assessed the prevalence of asymptomatic malaria infections with associated risk factors in an endemic district of Savannakhet province., Methods: In March 2024, a cross-sectional study was conducted in three villages of Nong District. Blood samples were collected from the fingertips of the participants for Plasmodium parasite identification using microscopy and Loop-mediated Isothermal Amplification (LAMP); those aged 13 years and above were also interviewed. Mann-Whitney U test and Fisher's exact test were performed to compare the medians of different age and temperature groups and determine the association between predictor variables and outcome variables respectively., Results: A total of 622 individuals participated in this survey; Plasmodium parasites were detected in 2.1% (13/622) of participants. The prevalence of asymptomatic malaria was 1.8% (11/622). Plasmodium vivax accounted for 15.4% (2/13) of all positive cases. The remaining species could not be identified. Farmers aged 15 years and above accounted for 81.8% of the asymptomatic infections. Ninety percent (90%) of the participants used bed nets in the village. Among interviewed participants, 23.6% reported not using mosquito bed nets in the forest; 21.3% of the participants who had been to the forest were nighttime forest workers., Conclusions: This study revealed a prevalence of 1.8% of asymptomatic malaria infections in the study areas with the majority of the infections clustered among farmers, and an important proportion of these populations not using protective measures in the forest. These findings showed that malaria reservoirs are notable with a lack of use of protective measures, which could threaten malaria control and elimination efforts. Therefore, malaria elimination in Lao PDR by 2030 would need interventions targeting high-risk adult populations for screening with sensitive tools coupled with sensitization on protective measures and asymptomatic malaria., Competing Interests: Declarations. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
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- 2025
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49. Self-amplifying mRNA vaccines protect elderly BALB/c mice against a lethal respiratory syncytial virus infection.
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Zuo L, Liu Q, Zhang K, Zhao L, Lin S, Dai Y, Sun Y, Li Y, Zhang P, Shen H, He D, Ma S, Long X, Chen Y, Luo Y, and Wong G
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- Animals, Mice, Disease Models, Animal, mRNA Vaccines immunology, Female, Respiratory Syncytial Viruses immunology, Respiratory Syncytial Viruses genetics, RNA, Messenger genetics, RNA, Messenger immunology, Humans, Immunoglobulin G immunology, Immunoglobulin G blood, Respiratory Syncytial Virus, Human immunology, Respiratory Syncytial Virus, Human genetics, Respiratory Syncytial Virus Infections prevention & control, Respiratory Syncytial Virus Infections immunology, Antibodies, Neutralizing immunology, Antibodies, Viral immunology, Respiratory Syncytial Virus Vaccines immunology, Respiratory Syncytial Virus Vaccines administration & dosage, Mice, Inbred BALB C, Viral Fusion Proteins immunology, Viral Fusion Proteins genetics
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Respiratory syncytial virus (RSV) represents a significant threat, being a primary cause of critical lower respiratory tract infections and fatalities among infants and the elderly worldwide, and poses a challenge to global public health. This urgent public health challenge necessitates the swift development of safe and effective vaccines capable of eliciting robust immune responses at low doses. Addressing this need, our study investigated five self-amplifying mRNA (sa-mRNA) candidate vaccines that encode the various pre-fusion conformations of the RSV fusion protein. When administered via low-dose intramuscular injection to 8-month-old elderly mice, these vaccines triggered potent humoral reactions and T helper type 1-biased cellular immunity. A prime-boost strategy followed by challenge with a lethal, mouse-adapted RSV strain showed that three of these sa-mRNA candidates achieved greater than 80% survival rates. An immune correlates of protection analysis contrasting immunized survivors with non-survivors suggest that the titers of IgG and neutralizing antibody are associated with vaccine-mediated protection from RSV infection. Our results highlight the usefulness of sa-mRNA vaccines to play a crucial role in forging an effective defense against RSV, addressing a critical need in protecting vulnerable populations against this virus., Competing Interests: Declaration of interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Inc.)
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- 2025
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50. Latent tuberculosis prevalence in healthcare workers in Laos: a cross-sectional study.
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Duangmixay S, Virachith S, Hübschen JM, Siphanthong P, Suthepmany S, Sayasone S, and Black AP
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Background: Individuals with latent tuberculosis infection (LTBI) have a high risk of active infection, morbidity and mortality. Healthcare workers are a group who have increased risk of infection and onward transmission to their patients and other susceptible individuals; however, LTBI is often undiagnosed, and individuals are asymptomatic. Interferon gamma release assays (IGRA) can detect evidence of TB infection in otherwise asymptomatic individuals and are a good indication of LTBI. Laos, a resource limited country in southeast Asia, has limited data on TB prevalence in the general population or in healthcare workers. This study aimed to estimate the prevalence of LTBI in Lao healthcare workers in Vientiane Capital., Methods: Healthcare workers from high-risk departments from 3 central hospitals in Laos were included (n = 196) and venous blood was tested by IGRA. A questionnaire was administered to determine their knowledge, attitude and practice towards TB and LTBI., Results: 10.2% of the participants were positive by IGRA, none of whom were previously aware of their TB status. The questionnaire revealed that knowledge and awareness of TB and LTBI were low., Discussion: A significant proportion of healthcare workers in this study had evidence of LTBI infection. These individuals were unaware of their TB status and we suggest that testing and treatment, as well as prevention strategies, should be routinely administered in Lao hospitals., Competing Interests: Declarations. Ethics approval and consent to participate: Ethics approval was obtained from the Lao National Ethics Committee for Health Research (Approval #34/NECHR). In addition, all participants received detailed explanations about the study and provided signed informed consent forms prior to their participation. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests., (© 2024. The Author(s).)
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- 2025
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