Search

Your search keyword '"Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE)"' showing total 11 results
Start Over Author "Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE)"
11 results on '"Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE)"'

1. Taxonomically informed metabolite annotation and data organization in natural products research

Author

B David, Jonathan Bisson, Miwa Dounoue-Kubo, Adriano Rutz, Joël Boustie, Simon Ollivier, D Olivier, LM Quiros Guerrero, J-L Wolfender, PM Allard, A Gaudry, Université de Lausanne (UNIL), Université de Genève (UNIGE), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), University of Chicago, Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE), Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, Université de Lausanne = University of Lausanne (UNIL), Université de Genève = University of Geneva (UNIGE), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
chemistry.chemical_compound, Annotation, chemistry, Metabolite, [CHIM]Chemical Sciences, Computational biology, Biology, Natural (archaeology), ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019
Full Text
View/download PDF

2. TRPV6 Is a Ca2+ Entry Channel Essential for Ca2+-induced Differentiation of Human Keratinocytes

Author

Benjamin Beck, Renata Polakowska, Natalia Prevarskaya, V’yacheslav Lehen’kyi, Maria Charveron, Roman Skryma, Pascal Bordat, Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate, Université de Lille, Sciences et Technologies-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 (JPArc), Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE), Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), and PIERRE FABRE-PIERRE FABRE

Subjects
Keratinocytes, Time Factors, TRPV6, [SDV]Life Sciences [q-bio], Cellular differentiation, TRPV Cation Channels, Biology, Cell morphology, Biochemistry, Cell Line, Calcitriol, medicine, Humans, Gene silencing, RNA, Messenger, RNA, Small Interfering, Molecular Biology, Involucrin, Cells, Cultured, Dose-Response Relationship, Drug, Cell Differentiation, Cell Biology, Cell biology, Phenotype, medicine.anatomical_structure, Cell culture, Calcium, Calcium Channels, Keratinocyte, Intracellular
Abstract

International audience; Ca(2+) is an essential factor inducing keratinocyte differentiation due to the natural Ca(2+) gradient in the skin. However, the membrane mechanisms that mediate calcium entry and trigger keratinocyte differentiation had not previously been elucidated. In this study we demonstrate that Ca(2+)-induced differentiation up-regulates both mRNA and protein expression of a transient receptor potential highly Ca(2+)-selective channel, TRPV6. The latter mediates Ca(2+) uptake and accounts for the basal [Ca(2+)](i) in human keratinocytes. Our results show that TRPV6 is a prerequisite for keratinocyte entry into differentiation, because the silencing of TRPV6 in human primary keratinocytes led to the development of impaired differentiated phenotype triggered by Ca(2+). The expression of such differentiation markers as involucrin, transglutaminase-1, and cytokeratin-10 was significantly inhibited by small interfering RNA-TRPV6 as compared with differentiated control cells. TRPV6 silencing affected cell morphology and the development of intercellular contacts, as well as the ability of cells to stratify. 1,25-Dihydroxyvitamin D3, a cofactor of differentiation, dose-dependently increased TRPV6 mRNA and protein expression in human keratinocytes. This TRPV6 up-regulation led to a significant increase in Ca(2+) uptake in both undifferentiated and differentiated keratinocytes. We conclude that TRPV6 mediates, at least in part, the pro-differentiating effects of 1,25-dihydroxyvitamin D3 by increasing Ca(2+) entry, thereby promoting differentiation. Taken together, these data suggest that the TRPV6 channel is a key element in Ca(2+)/1,25-dihydroxyvitamin D3-induced differentiation of human keratinocytes.

Published
2007
Full Text
View/download PDF

3. L’arginine orale, particulièrement sous une forme à libération prolongée, est utilisée plus efficacement pour la synthèse de monoxyde d’azote quand les sujets présentent des facteurs de risque du syndrome métabolique

Author

DEVEAUX, Ambre, Fouillet, Hélène, Petzke, Klaus, HERMIER, Dominique, André, Etienne, Bunouf, Pierre, Lantoine-Adam, Frédérique, Benamouzig, Robert, Mathé, Véronique, Huneau, Jean-François, Mariotti, François, Physiologie de la Nutrition et du Comportement Alimentaire (PNCA), AgroParisTech-Institut National de la Recherche Agronomique (INRA), German Institute of Human Nutrition Potsdam-Rehbrücke (DIfE), Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE), Institut de Recherche Pierre Fabre, AgroParisTech, Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Centre de Recherche Pierre Fabre, Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), and PIERRE FABRE-PIERRE FABRE

Subjects
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; Introduction et but de l'étude: La supplémentation orale en arginine a un effet bénéfique sur des fonctions associées au monoxyde d'azote (NO) chez des sujets avec des facteurs de risque cardiovasculaires. Cet effet semble dépendre de la dose et de l'augmentation de l'arginine plasmatique qui en résulte, mais aussi de la situation physiopathologique. Aucune donnée ne permet cependant de lier la mise à disposition de l'arginine et la synthèse de NO, en situation normale ou de risque cardiométabolique. L'objectif de cette étude était de comparer la biodisponibilité de l'arginine ingérée et son utilisation pour la synthèse de NO (1) selon qu'elle est consommée sous une forme à libération immédiate (LI) ou sous une forme à libération prolongée (LP) mimant la mise à disposition naturellement lente de l'arginine alimentaire, et (2) selon la présence de facteurs de risque du syndrome métabolique. Matériel et méthodes: Dans une étude avec répartition au hasard, selon un protocole croisé, nous avons comparé la biodisponibilité et l'utilisation pour la synthèse de NO d'arginine LP et LI (1,5g, 3 fois par jour) consommée pendant une semaine chez 15 sujets sains témoins et 12 sujets sains présentant un « tour de taille hypertriglycéridémique » (TTH). Nous avons pour cela utilisé de l'arginine marquée ([ 15 N 2-(guanido)]-arginine) et des dosages isotopiques plasmatiques et urinaires pour suivre l'apparition de l'arginine ingérée dans la circulation systémique et sa conversion spécifique en NO. Résultats et Analyse statistique: Au niveau cinétique, comme attendu, les augmentations des concentrations plasmatiques en arginine ingérée, en arginine totale et en ornithine totale étaient moins brutales et plus étalées après arginine-LP que LI. Au bilan, concernant les aires sous courbe (AUCs) plasmatiques sur 24h, l'AUC de l'arginine ingérée était moins importante après la supplémentation en arginine-LP que LI, tandis que les AUCs de l'arginine et ornithine totales étaient peu variables selon la forme (LP ou LI) et la durée (premier ou dernier jour) de la supplémentation. Par ailleurs, l'utilisation de l'arginine ingérée pour la synthèse de NO était plus élevée chez les sujets TTH que chez les témoins, et elle était plus élevée avec la forme LP qu'avec la forme LI, en particulier chez les sujets TTH (Ps

Published
2015

4. La supplémentation en L-arginine atténue la dysfonction endothéliale postprandiale lorsquel’argininémie basale est faible chez des sujets sains présentant des facteurs de risque dusyndrome métabolique

Author

Ambre Deveaux, Isabelle Pham, Sheila West, Etienne André, Frédérique Lantoine-Adam, Pierre Bunouf, Dominique Hermier, Véronique Mathé, Hélène Fouillet, Jean-François Huneau, Robert Benamouzig, François Mariotti, Physiologie de la Nutrition et du Comportement Alimentaire (PNCA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Departement de Physiologie et Explorations fonctionnelles, Hôpital Jean Verdier [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Departement of Nutritional Sciences, Pennsylvania State University (Penn State), Penn State System-Penn State System, Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE), Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, AgroParisTech-Institut National de la Recherche Agronomique (INRA), Hôpital Jean Verdier, and Institut de Recherche Pierre Fabre

Subjects
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

International audience; La supplémentation en L-arginine atténue la dysfonction endothéliale postprandiale lorsque l'argininémie basale est faible chez des sujets sains présentant des facteurs de risque du syndrome métabolique Veuillez choisir votre discipline: Clinique Présentation préférée: Communication orale Spécifiez votre âge

Published
2015

5. Cytisine-like alkaloids from Ormosia hosiei Hemsl.E.H. Wilson

Author

Isabelle Pouny, Sirong Yi, Muriel Batut, Laure Vendier, François Sautel, Bruno David, Georges Massiot, Paola B. Arimondo, Pharmacochimie de la Régulation Epigénétique du Cancer (ETaC), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-PIERRE FABRE, Laboratoire de chimie de coordination (LCC), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE), Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, Chongqing Institute of Medicinal Plant Cultivation, and China Academy of Chinese Medical Sciences

Subjects
Stereochemistry, Molecular Conformation, Plant Science, Horticulture, Ormosia hosiei, Receptors, Nicotinic, Crystallography, X-Ray, Biochemistry, Plant Roots, chemistry.chemical_compound, Cytisine, Alkaloids, [CHIM.COOR]Chemical Sciences/Coordination chemistry, Molecular Biology, Nuclear Magnetic Resonance, Biomolecular, Octane, biology, Molecular Structure, Plant Stems, Chemistry, Absolute configuration, Fabaceae, General Medicine, biology.organism_classification, Azocines, Nicotinic agonist, Alpha-4 beta-2 nicotinic receptor, Quinolizines
Abstract

International audience; Four alkaloids named hosieines A–D were isolated from the root and stem of Ormosia hosiei. Their flat structures were established by mass spectrometry and by a combination of NMR experiments. These molecules probably share a common biosynthetic origin with the lupin alkaloids but they differ in the formation of the last ring, being here part of a rare 2-azabicyclo[3.2.1]octane system. Their absolute configuration was determined by X-ray crystallography using CuKα radiation. As has been described for cytisine, they display a remarkable affinity towards neuronal nicotinic acetylcholine α4β2 receptor.

Published
2014
Full Text
View/download PDF

6. F 16915 prevents heart failure-induced atrial fibrillation: a promising new drug as upstream therapy

Author

Frédérique Lantoine-Adam, Philippe Legrand, Elisabeth Dupont-Passelaigue, Frédéric Longo, Stanley Nattel, Stéphane N. Hatem, Robert Létienne, Georghia Michael, Bruno Le Grand, Kunihiro Nishida, Monique David-Dufilho, Daniel Catheline, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE, UMRS 956, Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Cardiologie, Laboratoire de Biochimie et Nutrition Humaine, AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut de Recherche Pierre Fabre, Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE), UMR_S 1166, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Laboratoire de biochimie, Institut National de la Recherche Agronomique (INRA), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université-Sorbonne Université (SU), Department of Pharmacology and Therapeutics [Montréal], and McGill University = Université McGill [Montréal, Canada]

Subjects
Male, Drug, medicine.medical_specialty, Docosahexaenoic Acids, Pyridines, Heart Ventricles, media_common.quotation_subject, [SDV]Life Sciences [q-bio], Nicotinyl Alcohol, Heart failure, Rats, Sprague-Dawley, Dogs, Internal medicine, Occlusion, medicine, Animals, Prodrugs, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Heart Atria, Ventricular remodeling, ComputingMilieux_MISCELLANEOUS, media_common, Pharmacology, Ventricular Remodeling, business.industry, Atrial fibrillation, Atrial Remodeling, General Medicine, medicine.disease, 3. Good health, medicine.anatomical_structure, Docosahexaenoic acid, Cardiology, F 16915, business, Complication, Anti-Arrhythmia Agents, Artery
Abstract

Atrial fibrillation (AF) is a common complication of heart failure. The aim of the present study was to investigate the effects of a new pure docosahexaenoic acid derivative called F 16915 in experimental models of heart failure-induced atria dysfunction. The atrial dysfunction-induced AF was investigated (1) in a dog model of tachypacing-induced congestive heart failure and (2) in a rat model of heart failure induced by occlusion of left descending coronary artery and 2 months reperfusion. F 16915 (5 g/day for 4 weeks) significantly reduced the mean duration of AF induced by burst pacing in the dog model (989 +/- 111 s in the vehicle group to 79 +/- 59 s with F 16915, P < 0.01). This dose of F 16915 also significantly reduced the incidence of sustained AF (5/5 dogs in the vehicle group versus 1/5 with F 16915, P < 0.05). In the rat model, the percentage of shortening fraction in the F 16915 group (100 mg/kg p.o. daily) was significantly restored after 2 months (32.6 +/- 7.4 %, n = 9 vs 17.6 +/- 3.4 %, n = 9 in the vehicle group, P < 0.01). F 16915 also reduced the de-phosphorylation of connexin43 from atria tissue. The present results show that treatment with F 16915 reduced the heart dilation, resynchronized the gap junction activity, and reduced the AF duration in models of heart failure. Thus, F 16915 constitutes a promising new drug as upstream therapy for the treatment of AF in patients with heart failure.

Published
2014
Full Text
View/download PDF

7. Comparative study between reconstructed and native human epidermis using nuclear microscopy

Author

M. Rosdy, Philippe Moretto, Etienne Gontier, M.D. Ynsa, Alain Mavon, Dirk E. Boerma, Aurelio Climent-Font and Miguel Ángel Respaldiza, Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG), Université Sciences et Technologies - Bordeaux 1-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE), Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, SkinEthic Laboratories (SKINETHIC LABORATORIES), and SkinEthic Laboratories

Subjects
Nuclear and High Energy Physics, Microprobe, Micro pixe, Analytical chemistry, Human skin, 01 natural sciences, Skin models, 03 medical and health sciences, 0302 clinical medicine, 29.30.−Kv, 87.64.−t, Native skin, Instrumentation, integumentary system, Chemistry, 010401 analytical chemistry, Nuclear microscopy, 0104 chemical sciences, Micro-PIXE, Micro-RBS, Ultrastructure, Biophysics, STIM, [PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], Barrier effect, Epidermis, [SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology, 030215 immunology
Abstract

International audience; The physiological status of native skin is suffering from large inter-individual variations, especially in terms of inorganic ions content. For this reason, together with the advent of ethic laws on animal experimentation, reconstructed skin or epidermis models are extensively employed nowadays in penetration studies for cosmetic or pharmacological applications. It has been already verified that reconstructed human epidermis (RHE) has similar physiological mechanisms to native human skin, but until now, there are few studies where the elemental concentrations of both skins, reconstructed and native, are compared. In this work, freeze-dried thin sections of human native skin obtained from surgery have been characterized using PIXE, RBS and STIM at the CENBG nuclear microprobe. RHE samples were treated and analyzed in the same conditions for comparison. The combination of the different imaging and analysis techniques made possible a clear delimitation and identification of skin ultrastructure. The elemental concentrations of P, S, Cl, K and Ca were measured in the different strata. For both skins, concentrations have been compared and significant differences in terms of elemental concentrations have been determined using statistical approaches. Similar physiological characteristics were pointed out in both skin models, in particular the Ca gradient presumably involved in the regulation of the barrier effect.

Published
2006
Full Text
View/download PDF

8. Bioactive flavonoids of Tanacetum parthenium revisited

Author

Christophe Long, Frédéric Ausseil, Bruno David, Georges Massiot, Catherine Lavaud, Pierre Sauleau, Valerie Cassabois, Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE), Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, Laboratoire de Pharmacognosie, Université de Reims Champagne-Ardenne (URCA), Institut de pharmacologie et de biologie structurale (IPBS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
0106 biological sciences, Jaceidin, Flavonols, Flavonoid, Mitosis, Antineoplastic Agents, Plant Science, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, Horticulture, Biology, Chemical Fractionation, Tanacetum parthenium, 01 natural sciences, Biochemistry, chemistry.chemical_compound, Inhibitory Concentration 50, Botany, medicine, Inhibitory concentration 50, Animals, Molecular Biology, ComputingMilieux_MISCELLANEOUS, Confusion, chemistry.chemical_classification, Flavonoids, Centaureidin, Traditional medicine, 010405 organic chemistry, Plant Extracts, General Medicine, 0104 chemical sciences, 3. Good health, 010404 medicinal & biomolecular chemistry, chemistry, medicine.symptom, 010606 plant biology & botany
Abstract

Bio-guided fractionation of an extract from Tanacetum parthenium showing activity as mitotic blocker allowed the isolation and identification of santin 3, jaceidin 2 and centaureidin 1. The latter two closely related flavonols, which, to the best of our knowledge, are isolated here together for the first time, form a mixture difficult to resolve and which is probably the reason for the confusion in the literature regarding their occurrence. Centaureidin 1 had an IC50 of 1 microM while jaceidin 2 and santin 3 were 200 times less active.

Published
2003
Full Text
View/download PDF

9. The skin barrier function : a micro-PIXE study

Author

Marina Simon, Hervé Seznec, A. Mavon, Philippe Moretto, D. Bacqueville, Philippe Barberet, Le Noan, Ludovic, Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG), Université Sciences et Technologies - Bordeaux 1-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE), Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, Université Sciences et Technologies - Bordeaux 1, and Institut de Recherche Pierre Fabre - Laboratoire de Pharmacocinétique Cutanée

Subjects
Micro pixe, Chemical compound, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Skin physiology, Analytical chemistry, Human skin, 030207 dermatology & venereal diseases, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, NUCLEAR MICROPROBE, PERCUTANEOUS-ABSORPTION, EPIDERMIS, IN-VIVO, Spectroscopy, Barrier function, Skin barrier function, 030304 developmental biology, DAMAGE, 0303 health sciences, [PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Chemistry, STRATUM-CORNEUM, Penetration (firestop), 3. Good health, MICROANALYSIS, Ion homeostasis, Biophysics
Abstract

Human body is perpetually subjected to environmental exposures as sun, natural, urban or industrial pollutions and societal exposures. Skin is the main physiological barrier facing to these different stresses; however its response mechanisms are still not well understood. Skin models are thus necessary to understand skin physiology and behavior in normal conditions or under stress. Because of the large natural interindividual variability and of some difficulties in obtaining human skin biopsies, we have been developing and characterizing different skin models such as reconstructed epidermis in vitro, native pigskin and human keratinocyte cells. These models allow us to study the mechanisms involved in ion homeostasis in correlation with structural organization and biological responses. In the present study, we have characterized the pig ear skin model using micro-particle induced x-ray emission (PIXE) analysis with the aim of evaluating the distribution of inorganic ions under normal or stress conditions together with the transepidermal penetration of external agents, such as aluminum oxides. The nuclear microprobe analysis allowed us to validate the culture conditions of the tissue and we have shown an alteration of the inorganic ion patterns after exposure to a chemical compound (sodium dodecyl sulfate) known for its deleterious effects on the barrier function. Finally, we have shown no transepidermal penetration of aluminum oxides after different exposures to cosmetic formulations. Copyright (C) 2009 John Wiley & Sons, Ltd.

Full Text
View/download PDF

10. L-arginine supplementation alleviates post-prandial endothelial dysfunction when baseline fasting plasma arginine concentration is low: a randomized controlled trial in healthy subjects with risk factors for metabolic syndrome

Author

Deveaux, A., Pham, I., West, S. G., Andre, E., Lantoine-Adam, F., Bunouf, P., Sadi, S., Hermier, D., Mathe, V., Helene Fouillet, Huneau, J. F., Benamouzig, R., Mariotti, F., Physiologie de la Nutrition et du Comportement Alimentaire (PNCA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Nord (Paris 13), Department of Nutritional Sciences, Pennsylvania State University (Penn State), Penn State System-Penn State System, Penn State System, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE, Centre de Recherche en Nutrition Humaine, Federation of European Physiological Societies., French Physiological Society., AgroParisTech-Institut National de la Recherche Agronomique (INRA), Hypoxie et Poumon : pneumopathologies fibrosantes, modulations ventilatoires et circulatoires (H&P), Université Paris 13 (UP13)-UFR SMBH, Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE), Institut de Recherche Pierre Fabre, and PIERRE FABRE-PIERRE FABRE

Subjects
[SDV]Life Sciences [q-bio], [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

11. Développement de modèles de peau in vitro/ex vivo et caractérisation en réponse à des expositions physiques et chimiques. Application à l'étude de la fonction de barrière cutanée en relation avec la pénétration transcutanée d'oxydes métalliques et de nanoparticules

Author

Simon, M., Barberet, Ph., Moretto, Ph., Bacqueville, D., Mavon, A., Seznec, H., Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG), Université Sciences et Technologies - Bordeaux 1-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche Pierre FABRE (INSTITUT DE RECHERCHE PIERRE FABRE), Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, and Le Noan, Ludovic

Subjects
[PHYS.PHYS.PHYS-MED-PH] Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], [PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], [PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph]

Catalog

Books, media, physical & digital resources
See catalog results