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1. One-carbon metabolism and global DNA methylation in mothers of individuals with Down syndrome

Author

Cristiani Cortez Mendes, Helio Vannucchi, Marcos N. Eberlin, Joice Matos Biselli, Maria Francesca Riccio, Bruna Lancia Zampieri, Érika Cristina Pavarino, Matias Eliseo Melendez, Valdemir Melechco Carvalho, Eny Maria Goloni-Bertollo, Lidia Maria Rebolho Batista Arantes, André Lopes Carvalho, Faculdade de Medicina de São José do Rio Preto-FAMERP, Hospital Israelita Albert Einstein, Barretos Cancer Hospital, Universidade Estadual Paulista (UNESP), Fé e Sociedade, Instituto de Pesquisa Dr. Domingos A. Boldrini, Universidade de São Paulo (USP), and Fleury-Centro de Medicina Diagnóstica

Subjects
Adult, Male, Cancer Research, Down syndrome, Adolescent, Aneuploidy, Alu element, Mothers, Biology, Genomic Instability, Young Adult, Folic Acid, Alu Elements, medicine, Humans, Long interspersed nucleotide elements, Genetic Predisposition to Disease, Alu elements, Aged, Genetics, Transcobalamins, DNA methylation, Polymorphism, Genetic, Cell Biology, Methylation, Thymidylate Synthase, DNA Methylation, Middle Aged, medicine.disease, Carbon, Mother-Child Relations, Long Interspersed Nucleotide Elements, Betaine-Homocysteine S-Methyltransferase, Female, Down Syndrome, Chromosome 21, Trisomy, DNA hypomethylation, Genome-Wide Association Study, Signal Transduction
Abstract

Made available in DSpace on 2022-04-29T08:32:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-01 Down syndrome (DS) is the most common chromosomal disorder, resulting from the failure of normal chromosome 21 segregation. Studies have suggested that impairments within the one-carbon metabolic pathway can be of relevance for the global genome instability observed in mothers of individuals with DS. Based on the association between global DNA hypomethylation, genome instability, and impairments within the one-carbon metabolic pathway, the present study aimed to identify possible predictors, within the one-carbon metabolism, of global DNA methylation, measured by methylation patterns of LINE-1 and Alu repetitive sequences, in mothers of individuals with DS and mothers of individuals without the syndrome. In addition, we investigated one-carbon genetic polymorphisms and metabolites as maternal predisposing factors for the occurrence of trisomy 21 in children. Eighty-three samples of mothers of children with DS with karyotypically confirmed free trisomy 21 (case group) and 84 of mothers who had at least one child without DS or any other aneuploidy were included in the study. Pyrosequencing assays were performed to access global methylation. The results showed that group affiliation (case or control), betaine-homocysteine methyltransferase (BHMT) G742A and transcobalamin 2 (TCN2) C776G polymorphisms, and folate concentration were identified as predictors of global Alu DNA methylation values. In addition, thymidylate synthase (TYMS) 28-bp repeats 2R/3R or 3R/3R genotypes are independent maternal predisposing factors for having a child with DS. This study adds evidence that supports the association of impairments in the one-carbon metabolism, global DNA methylation, and the possibility of having a child with DS. Unidade de Pesquisa em Genética e Biologia Molecular-UPGEM Departamento de Biologia Molecular Faculdade de Medicina de São José do Rio Preto-FAMERP Hospital Israelita Albert Einstein Molecular Oncology Research Center Barretos Cancer Hospital Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto Departamento de Ciências Biológicas Universidade Presbiteriana Mackenzie Discovery-Mackenzie-Núcleo Mackenzie de Pesquisa Núcleo Mackenzie de Pesquisas em Ciência Fé e Sociedade Instituto de Pesquisa Dr. Domingos A. Boldrini Laboratório de Nutrição Departamento de Clínica Médica Faculdade de Medicina de Ribeirão Preto-USP Fleury-Centro de Medicina Diagnóstica Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto Departamento de Ciências Biológicas

Published
2021

2. One-carbon metabolism and global DNA methylation in mothers of individuals with Down syndrome.

Author

Mendes CC, Zampieri BL, Arantes LMRB, Melendez ME, Biselli JM, Carvalho AL, Eberlin MN, Riccio MF, Vannucchi H, Carvalho VM, Goloni-Bertollo EM, and Pavarino ÉC

Subjects
Adolescent, Adult, Aged, Alu Elements genetics, Betaine-Homocysteine S-Methyltransferase genetics, Betaine-Homocysteine S-Methyltransferase metabolism, Female, Folic Acid metabolism, Genetic Predisposition to Disease genetics, Humans, Long Interspersed Nucleotide Elements genetics, Male, Middle Aged, Polymorphism, Genetic, Signal Transduction genetics, Signal Transduction physiology, Thymidylate Synthase genetics, Transcobalamins genetics, Transcobalamins metabolism, Young Adult, Carbon metabolism, DNA Methylation genetics, Down Syndrome genetics, Down Syndrome metabolism, Genome-Wide Association Study, Genomic Instability genetics, Mother-Child Relations, Mothers
Abstract

Down syndrome (DS) is the most common chromosomal disorder, resulting from the failure of normal chromosome 21 segregation. Studies have suggested that impairments within the one-carbon metabolic pathway can be of relevance for the global genome instability observed in mothers of individuals with DS. Based on the association between global DNA hypomethylation, genome instability, and impairments within the one-carbon metabolic pathway, the present study aimed to identify possible predictors, within the one-carbon metabolism, of global DNA methylation, measured by methylation patterns of LINE-1 and Alu repetitive sequences, in mothers of individuals with DS and mothers of individuals without the syndrome. In addition, we investigated one-carbon genetic polymorphisms and metabolites as maternal predisposing factors for the occurrence of trisomy 21 in children. Eighty-three samples of mothers of children with DS with karyotypically confirmed free trisomy 21 (case group) and 84 of mothers who had at least one child without DS or any other aneuploidy were included in the study. Pyrosequencing assays were performed to access global methylation. The results showed that group affiliation (case or control), betaine-homocysteine methyltransferase (BHMT) G742A and transcobalamin 2 (TCN2) C776G polymorphisms, and folate concentration were identified as predictors of global Alu DNA methylation values. In addition, thymidylate synthase (TYMS) 28-bp repeats 2R/3R or 3R/3R genotypes are independent maternal predisposing factors for having a child with DS. This study adds evidence that supports the association of impairments in the one-carbon metabolism, global DNA methylation, and the possibility of having a child with DS., (© 2021. Japan Human Cell Society.)

Published
2021
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