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The available prophylactic vaccines for human papillomavirus (HPV) in the market are only effective against specific types of HPV, rendering them ineffective for other types of HPV infections. The objective of this research is to investigate the stability of the recombinant protein constructed, namely chimeric L1/L2 protein from HPV type 52, with improved cross-neutralization ability. The 3D model, predicted using Alphafold, Robetta, I-Tasser, and refined with Galaxy Refinement, is validated using Ramachandran plot analysis. The stability is verified through molecular dynamics simulations, considering parameters such as RMSD, RMSF, Rg, and SASA, where stable conditions are observed. The chimeric L1/L2 protein from HPV type 52 is purified using affinity chromatography, and the His-tag is cleaved using SUMO protease to obtain pure chimeric protein with the size of ~ 55 kDa. Western blot analysis confirms binding to anti-L1 HPV type 52 polyclonal antibody. The obtained vaccine candidate can be utilized as an effective prophylactic vaccine against HPV. [ABSTRACT FROM AUTHOR]

Diabetes affects 537 million adults globally, with 19.47 million cases in Indonesia. It arises from insufficient or ineffective insulin production, leading to complications like nephropathy. Due to the adverse effects of conventional medications, there is a growing need for safer alternatives. Functional foods and bioactive compounds show promise in managing diabetes. One such alternative is rice. Indonesia boasts a unique rice variety known as Pulu Mandoti, exclusively cultivated in the Enrekang District, Makassar, Indonesia. This delightful red rice variant offers numerous nutritional benefits. Unlike white rice, red rice is abundant in essential nutrients such as calcium, zinc, magnesium, protein, and fiber. The study focused on Pulu Mandoti, exploring its potential for antidiabetic and antioxidant activities using LC-MS analysis. Twelve (12) compounds were identified within the 1.23-14.41-min retention time range, with Compound 11 (2,2'-methylenebis(dibenzo[b,d]thiophene)) showing the strongest antioxidant potential compared to vitamin C. In molecular docking, Compound 11 exhibited the lowest binding affinity for both alpha-amylase and alpha-glucosidase (-10.5 and -8.7 kcal/mol, respectively), whereas acarbose displayed the highest binding affinity. For antioxidant analysis, Compounds 11 and 5 demonstrated the lowest binding affinities for NADPH oxidase and xanthine oxidoreductase, respectively, whereas vitamin C showed the highest. The coefficient values for drug properties similarity ranged from 0.40-0.76 for antidiabetic drugs, with Compound 5 displaying the highest coefficient value (0.76) and from 0.41-0.68 for antioxidants, with the highest value observed for antioxidant peptide A acetate. [ABSTRACT FROM AUTHOR]

Enzymatic hydrolysis is more efficient compared to other types of protein hydrolysis methods due to the fact that enzymes function as specific catalysts that can produce peptides in a less complex form and avoid non-hydrolytic changes. Extracellular protease from lactococcus lactis subsp. lactis (LAC3) has the potential as an agent for enzymatic hydrolysis of marine products, especially for non-fibrillar and non-collagen protein types. Extracellular protease from LAC3 was successfully isolated and was able to hydrolyze sea cucumber and lorjuk shellfish. Hydrolyzed products appear to have molecular weights of 4.6 and 15 kDa. The hydrolysates from the enzymatic hydrolysis of the two marine samples have significant antioxidant activities based on the DPPH inhibition assay. The best 3D protein structure modeling was done using the AlphaFold web server. [ABSTRACT FROM AUTHOR]