8 results on '"Irma Bauer"'
Search Results
2. Expression quantitative trait locus fine mapping of the 17q12–21 asthma locus in African American children: a genetic association and gene expression study
- Author
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Carole Ober, Chris G McKennan, Kevin M Magnaye, Matthew C Altman, Charles Washington, Catherine Stanhope, Katherine A Naughton, Mario G Rosasco, Leonard B Bacharier, Dean Billheimer, Diane R Gold, Lisa Gress, Tina Hartert, Suzanne Havstad, Gurjit K Khurana Hershey, Brian Hallmark, D Kyle Hogarth, Daniel J Jackson, Christine C Johnson, Meyer Kattan, Robert F Lemanske, Susan V Lynch, Eneida A Mendonca, Rachel L Miller, Edward T Naureckas, George T O'Connor, Christine M Seroogy, Ganesa Wegienka, Steven R White, Robert A Wood, Anne L Wright, Edward M Zoratti, Fernando D Martinez, Dennis Ownby, Dan L Nicolae, Albert M Levin, James E Gern, Niek Achten, John Ainsworth, Nonna Akkerman, Elizabeth Anderson, Larry J. Anderson, Howard Andrews, Elizabeth Armagost, Mary Ann Aubuchon, Julia Bach, Leonard Bacharier, Kathrine L. Barnes, Charles Barone, Irma Bauer, Paloma Beamer, Patrice Becker, Alyssa Bednarek, Stacey Bellemore, Casper G. Bendixsen, Jocelyn M. Biagini Myers, Christine Billstrand, Geraldine Birg, Shirley Blocki, Gordon Bloomberg, Kevin Bobbitt, Yury Bochkov, Karen Bourgeois, Homer Boushey, Rebecca Brockman-Schneider, Steven M. Brunwasser, Richard Budrevich, Jeffrey W. Burkle, William Busse, Agustin Calatroni, Janice Campbell, Kirsten Carlson-Dakes, Andrea Cassidy-Bushrow, James D. Chappell, Deborah Chasman, Teresa M. Chipps, Tatiana Chirkova, Deanna Cole, Alexandra Connolly, Michelle Cootauco, Kaitlin Costello, Philip Couch, Brent Coull, Mark Craven, Gina Crisafi, William Cruikshank, Kristi Curtsinger, Adnan Custovic, Suman R. Das, Douglas DaSilva, Soma Datta, Brent Davidson, Lydia De La Ossa, Mark DeVries, Qian Di, Samara Dixon, Erin Donnerbauer, Marian Dorst, Susan Doyle, Amy Dresen, William D. Dupont, Janet Durrange, Heidi Erickson, Michael D. Evans, Jerel Ezell, Leanna Farnham, Roxanne Filardo-Collins, Salvatore Finazzo, Zachary Flege, Conner Fleurat, Heather Floerke, Dorothy Floerke, Terry Foss, Angela Freie, Wayne Frome, Samantha Fye, Lisa Gagalis, Rebecca Gammell, Ronald E. Gangnon, James E. Ge, Tebeb Gebretsadik, Peter Gergen, James E. Gern, Heike Gibson, Edlira Gjerasi, Diane R. Gold, Nicole Gonzalez, Kayla Goodman, Kristine Grindle, Taylor Groeschen, Marilyn Halonen, Jaime Hart, Tina V. Hartert, Patrick Heinritz, Sharon Hensley Alford, Julie Herbstman, Kellie Hernandez, Lori Hoepner, Daniel J. Jackson, Samadhan J. Jadhao, Katy Jaffee, Peter James, Jacqueline Jezioro, Marcia Jimenez Pescador, Christine C. Johnson, Tara Johnson, Camille Johnson, Amelia Jones, Kyra Jones, Paul Jones, Carolina Jordan, Christine LM Joseph, Kristina Keidel, Matthew C. Keifer, Rick Kelley, Gurgit K. Khurana Hershey, Haejin Kim, Itai Kloog, Tammy Kronenwetter Koepel, Clint Koerkenmeier, Laura Ladick, Carin Lamm, Emma Larkin, Howard Lederman, Aviva Lee-Parritz, Stephanie Leimenstoll, Robert F. Lemanske, Jr., Grace K. LeMasters, Albert M. Levin, Jessica Levine, Xinhua Liu, Zhouwen Liu, Silvia Lopez, Nathan Lothrop, Stephanie Lovinsky-Desir, Nicholas Lukacs, Susan Lynch, Christian Lynch, Erik Mann, Jennifer Martin, Lisa Martin, Fernando D. Martinez, Elizabeth Matsui, Katherine McCauley, Megan Mccollum, Judith McCullough, Chris G. McKennon, Jennifer Meece, Eneida Mendonca, Lance Mikus, Rachel L. Miller, Patricia Minton, Herman Mitchell, Vicki Moon, Paul E. Moore, Wayne Morgan, Valerie Morgan, David Morgan, Liza Murrison, Charlotte Nicholas, Daniel Nicolae, Adam Nunez, George O'Connor, Sharon O'Toole, Brent F. Olson, Irene Ong, Sarah Osmundson, Tressa Pappas, Frederica Perera, Matthew Perzanowski, Edward Peterson, Marcela Pierce, Penny Price-Johnson, Victoria Rajamanickam, Judyth Ramirez, Kimberly Ray, Megan Renneberg, Weeberb Requia, Kylie Riley, Janelle Rivera, Neisha Rivers, Kathy Roberg, Theresa Rogers, Christian Rosas-Salazar, Pat Russell, Patrick H. Ryan, Yoel Sadovsky, Lisa Salazar, Hugh Sampson, Megan Sandel, Nathan Schoettler, Joel Schwartz, Dena Scott, Christine M. Seroogy, Renee Sharp, Meghan H. Shilts, Steve Sigelman, Anne Marie Singh, Alexandra Sitarik, Ernestine Smartt, Ronald Sorkness, Christine Sorkness, Amber Spangenberg, Rhoda Sperling, David Spies, Debra A. Stern, Brandy Stoffel, R. Stokes Peebles, Gina Stouffer, Cathey Strauchman Boyer, Caitlin Suddeuth, Umberto Tachinardi, Deliang Tang, Zhengzheng Tang, Jena Tate, William Taylor, Krista Tensing, Elizabeth Tesson, Kathy Thompson, Emma Thompson, Christopher Tisler, Alkis Togias, Kedir Turi, Victoria Turner, Marina Tuzova, Jeffrey J. VanWormer, Cynthia M. Visness, Rose Vrtis, Anthony Wahlman, Lena Wang, Karen Wells, William Wentworth-Sheilds, Lisa Wheatley, Nitsa Whitney, L. Keoki Williams, Frank Witter, Christopher Wolfe, Robert A. Wood, Kimberley Woodcroft, Kim B. Woodward, Anne L. Wright, Rosalind Wright, Pingsheng Wu, Melissa Yaeger, Perri Yaniv, Antonella Zanobetti, Shirley Zhang, Patricia Zook, Edward M. Zoratti, and Academic Medical Center
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Pulmonary and Respiratory Medicine ,Male ,Candidate gene ,Linkage disequilibrium ,Genotype ,Quantitative Trait Loci ,Single-nucleotide polymorphism ,Locus (genetics) ,Quantitative trait locus ,Polymorphism, Single Nucleotide ,Linkage Disequilibrium ,White People ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,Humans ,Genetic Predisposition to Disease ,030212 general & internal medicine ,Allele ,Child ,Genetic Association Studies ,Genetic association ,Genetics ,business.industry ,Gene Expression Profiling ,Membrane Proteins ,Epithelial Cells ,Asthma ,United States ,Neoplasm Proteins ,Black or African American ,030228 respiratory system ,Expression quantitative trait loci ,Leukocytes, Mononuclear ,Female ,business ,Chromosomes, Human, Pair 17 - Abstract
Background: African ancestry is associated with a higher prevalence and greater severity of asthma than European ancestries, yet genetic studies of the most common locus associated with childhood-onset asthma, 17q12–21, in African Americans have been inconclusive. The aim of this study was to leverage both the phenotyping of the Children's Respiratory and Environmental Workgroup (CREW) birth cohort consortium, and the reduced linkage disequilibrium in African Americans, to fine map the 17q12–21 locus. Methods: We first did a genetic association study and meta-analysis using 17q12–21 tag single-nucleotide polymorphisms (SNPs) for childhood-onset asthma in 1613 European American and 870 African American children from the CREW consortium. Nine tag SNPs were selected based on linkage disequilibrium patterns at 17q12–21 and their association with asthma, considering the effect allele under an additive model (0, 1, or 2 effect alleles). Results were meta-analysed with publicly available summary data from the EVE consortium (on 4303 European American and 3034 African American individuals) for seven of the nine SNPs of interest. Subsequently, we tested for expression quantitative trait loci (eQTLs) among the SNPs associated with childhood-onset asthma and the expression of 17q12–21 genes in resting peripheral blood mononuclear cells (PBMCs) from 85 African American CREW children and in upper airway epithelial cells from 246 African American CREW children; and in lower airway epithelial cells from 44 European American and 72 African American adults from a case-control study of asthma genetic risk in Chicago (IL, USA). Findings: 17q12–21 SNPs were broadly associated with asthma in European Americans. Only two SNPs (rs2305480 in gasdermin-B [GSDMB] and rs8076131 in ORMDL sphingolipid biosynthesis regulator 3 [ORMDL3]) were associated with asthma in African Americans, at a Bonferroni-corrected threshold of p
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- 2020
3. Fluorescence Polarization in Homogeneous Nucleic Acid Analysis II: 5′-Nuclease Assay
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Irma Bauer-Sardiña, Sherif Medhat Latif, Kenneth J. Livak, Kostubh Ranade, and Pui-Yan Kwok
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Nuclease ,Chromatography ,Nucleic acid quantitation ,Fluorescence Polarization ,Sequence Analysis, DNA ,Biology ,Polymerase Chain Reaction ,Fluorescence ,chemistry.chemical_compound ,Spectrometry, Fluorescence ,Biochemistry ,chemistry ,Sequence Homology, Nucleic Acid ,Methods ,Genetics ,TaqMan ,Nucleic acid ,biology.protein ,Humans ,Molecule ,Taq Polymerase ,Genetics (clinical) ,Fluorescence anisotropy ,Taq polymerase - Abstract
When the temperature and viscosity of the solvent is held constant, the degree of fluorescence polarization (FP) detected when a fluorescent dye is excited by plane polarized light depends mostly on the molecular weight of the dye molecule. By monitoring the FP of a fluorescent dye molecule, one can detect significant changes in the molecular weight of a fluorescent molecule without separation or purification. The 5′-nuclease (TaqMan) assay is a robust single nucleotide polymorphism genotyping method where an allele-specific probe that binds to a perfectly complementary target is cleaved by the 5′-nuclease activity of Taq DNA polymerase. Because the TaqMan probe is labeled with a fluorescent dye, it has high FP value when intact but a low FP value after cleavage. In this study, we compared the results of the 5′-nuclease assay based on standard fluorescence intensity readings and FP readings when genotyping 90 individuals with 20 single nucleotide polymorphisms. Our results show that FP is just as robust and reliable as the standard fluorescence detection method. Use of FP detection makes it possible to reduce the cost of TaqMan probes by abrogating the need for a fluorescence quencher.
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- 2001
4. Juxtaposed regions of extensive and minimal linkage disequilibrium in human Xq25 and Xq28
- Author
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Pui-Yan Kwok, Antonio Cao, Jenna Putzel, Giuseppe Pilia, Irma Bauer-Sardiña, Patricia Taillon-Miller, Juha Kere, Tarja Laitinen, John P. Rice, and Nancy L. Saccone
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Genetics ,0303 health sciences ,Linkage disequilibrium ,education.field_of_study ,Population ,Haplotype ,Biology ,Xq28 ,03 medical and health sciences ,0302 clinical medicine ,Genetic distance ,Gene mapping ,Genetic marker ,Allele ,education ,030217 neurology & neurosurgery ,030304 developmental biology - Abstract
Linkage disequilibrium (LD), or the non-random association of alleles, is poorly understood in the human genome1. Population genetic theory suggests that LD is determined by the age of the markers, population history, recombination rate, selection and genetic drift2. Despite the uncertainties in determining the relative contributions of these factors, some groups have argued that LD is a simple function of distance between markers3,4. Disease-gene mapping studies and a simulation study gave differing predictions on the degree of LD in isolated and general populations5,6. In view of the discrepancies between theory and experimental observations, we constructed a high-density SNP map of the Xq25–Xq28 region7 and analysed the male genotypes and haplotypes across this region for LD in three populations. The populations included an outbred European sample (CEPH males) and isolated population samples from Finland and Sardinia. We found two extended regions of strong LD bracketed by regions with no evidence for LD in all three samples. Haplotype analysis showed a paucity of haplotypes in regions of strong LD. Our results suggest that, in this region of the X chromosome, LD is not a monotonic function of the distance between markers, but is more a property of the particular location in the human genome.
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- 2000
5. Tu1141 Effects of Gastric Bypass in Obese Patients With Barrett's Esophagus
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Gaston Borlle, Carolina Bolino, Irma Bauer, Jean M. Dumonceau, Oscar Brasesco, Luis Durand, Luis E. Caro, Cecilio L. Cerisoli, Guadalupe Dova, Catherina Gajardo, and Julieta Paleari
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medicine.medical_specialty ,business.industry ,General surgery ,Gastric bypass ,Gastroenterology ,030209 endocrinology & metabolism ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Barrett's esophagus ,Medicine ,030211 gastroenterology & hepatology ,Radiology, Nuclear Medicine and imaging ,business - Published
- 2016
6. Improved enzyme immunosorbent assay for mouse prolactin using penicillinase as label
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Robert A. Good, Noorbibi K. Day, Robert W. Engleman, Yoshifumi Tomita, and Irma Bauer-Sardina
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endocrine system ,Dopamine Agents ,Immunology ,Radioimmunoassay ,Enzyme-Linked Immunosorbent Assay ,Biology ,Absorbance ,Mice ,Microtiter plate ,Pituitary Gland, Anterior ,medicine ,Animals ,Immunology and Allergy ,chemistry.chemical_classification ,Substrate (chemistry) ,Penicillinase ,Molecular biology ,Prolactin ,Penicillin ,Enzyme ,chemistry ,Aminoquinolines ,biology.protein ,Antibody ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Enzyme immunosorbent assay (EIA) for mouse prolactin was established by modifying a method originally developed for human prolactin by Shrivastav et al. This simple, sensitive, rapid, and reproducible assay utilizes penicillinase as the labeling enzyme, rabbit anti-mouse prolactin antibody (Ab) and goat anti-rabbit Ig Ab as the first and second antibodies. Prolactin reference preparations and enzyme-conjugated prolactin were mixed with the first Ab and incubated for 0.5 h at 4 degrees C (24-48 h for serum samples). Then, the sample mixture was transferred to the wells of microtiter plate coated with the second Ab. After being kept at room temperature for 2 h, the plate was washed and filled with substrate solution (penicillin V). Absorbance at 620 nm was measured with an ELISA reader to quantitate the amount of conjugated prolactin bound to the second Ab. The prolactin levels obtained by this assay exhibited good correlation with those measured by radioimmunoassay (RIA) (y = 0.95x + 9.14, r = 0.943), and the sensitivity of EIA was equivalent to that of RIA (1.7 ng/ml). The CVs of intra-assay and inter-assay by EIA for mouse serum samples ranged comparably to those by RIA.
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- 1992
7. Calorie Consumption Level Influences Development of C3H/Ou Breast Adenocarcinoma with Indifference to Calorie Source
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Yoshifumi Tomita, Rou-Fuie Chen, Robert A. Good, My Lien Dao, Robert W. Engelman, Irma Bauer-Sardina, and Noorbibi K. Day
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Aging ,medicine.medical_specialty ,Calorie ,Ratón ,Mammary gland ,Antigen-Antibody Complex ,Adenocarcinoma ,Biology ,Lymphocyte Activation ,General Biochemistry, Genetics and Molecular Biology ,Mice ,Internal medicine ,medicine ,Animals ,Lymphocytes ,Risk factor ,Mice, Inbred C3H ,Mouse mammary tumor virus ,Mammary Neoplasms, Experimental ,medicine.disease ,biology.organism_classification ,Prolactin ,Diet ,Killer Cells, Natural ,medicine.anatomical_structure ,Endocrinology ,biology.protein ,Interleukin-2 ,Female ,Antibody ,Energy Intake - Abstract
To analyze simultaneously the influence attributable to calorie consumption level and percentage of dietary fat on the spontaneous development of mammary adenocarcinoma, virgin female C3H/Ou mice were separated into five dietary groups. Four groups of mice were fed purified diets either ad libitum (16-18 kcal/mouse/day) or restricted 40% in calorie consumption (10-11 kcal/mouse/day), and diets contained either 4.5%, 7.5%, 67%, or 68% calories from fat. Mice that consumed isocaloric diets developed breast malignancy at a comparable pace. Consuming a diet in which fats were present only at levels sufficient to satisfy the threshold requirement of essential fatty acids, 4.5-7.5% of the total calories, or alternatively where dietary fat represented greater than 67% of the total calories consumed, did not significantly alter the tendency for breast tumor development. The pace and frequency with which tumors occurred reflected the host's level of calorie consumption. Mice consuming a high caloric diet, low or high in fat, tended to have a shortened latency to breast tumor formation, an increased incidence of breast tumors, elevated serum prolactin levels, elevated levels of antibodies to mouse mammary tumor virus, and elevated circulating immune complex levels.
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- 1990
8. The homozygous complete hydatidiform mole: a unique resource for genome studies
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Patricia Taillon-Miller, Hamideh Zakeri, LaDeana W. Hillier, David G. Mutch, Irma Bauer-Sardiña, and Pui-Yan Kwok
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Genetic Markers ,Male ,Population ,Black People ,Single-nucleotide polymorphism ,Biology ,Genome ,Polymerase Chain Reaction ,White People ,Gene mapping ,Gene Frequency ,Pregnancy ,Genetics ,Humans ,Choriocarcinoma ,education ,Allele frequency ,BRCA2 Protein ,education.field_of_study ,Polymorphism, Genetic ,Genome, Human ,Haplotype ,Homozygote ,Chromosome ,Hydatidiform Mole ,Neoplasm Proteins ,Haplotypes ,Human genome ,Female ,Transcription Factors - Abstract
The most frequent type of complete hydatidiform mole is a 46, XX homozygote formed by the fertilization of an empty ovum by a single haploid sperm that later duplicates its chromosomes to give a diploid tumor. The homozygous nature of these complete hydatidiform moles makes them unique resources for human genome studies. They can serve as homozygous controls in the development of single nucleotide polymorphism (SNP) markers and provide a way to obtain long-range haplotypes that are useful in population studies. The use of a homozygous control makes it possible to estimate the allele frequencies of the SNP markers in any population by sequencing pooled DNA samples. In this report, we present evidence of homozygosity of a complete hydatidiform mole using 20 diallelic markers distributed across the genome. Furthermore, its usefulness as a homozygous control in SNP development and as a resource for long-range haplotype determination is demonstrated using 11 newly discovered loci in the BRCA2 region on chromosome 13q12-q13.
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- 1998
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