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Your search keyword '"Ishqi, Hassan Mubarak"' showing total 35 results
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35 results on '"Ishqi, Hassan Mubarak"'

7. Racial Disparity Between Whites and African Americans in Incidence and Outcome of Pancreatic Cancer: Have We Made a Difference?

Author

Amirian, Haleh, primary, Kwon, Deukwoo, additional, Vickers, Selwyn M., additional, Livingstone, Alan, additional, Jayaweera, Dushyantha, additional, Saluja, Ashok K., additional, Dawra, Rajinder, additional, Gupta, Vineet Kumar, additional, Charles Jacob, Harrys Kishore, additional, Modi, Shrey, additional, Kir, Devika, additional, Lavania, Shweta Pankajkumar, additional, Ishqi, Hassan Mubarak, additional, Pandey, Somnath, additional, and Middleton, Ashley Nicole, additional

Published
2022
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24. Elucidating the interaction of sulindac with calf thymus DNA: biophysical and in silico molecular modelling approach.

Author

Husainpresent Address: Department Of Biosciences Jamia Millia Islamia New Delhi-110025 India., Mohammed Amir, Ishqi, Hassan Mubarak, Rehman, Sayeed Ur, Sarwar, Tarique, Afrin, Shumaila, Rahman, Yusra, and Tabish, Mohammad

Subjects
*SULINDAC, *DRUG-DNA interactions, *MOLECULAR models, *GENE expression, *FLUORESCENCE, *ENTHALPY
Abstract

Sulindac is one of the most effective non-steroidal anti-inflammatory drugs (NSAIDs) and belongs to the arylalkanoic acid class. DNA is the major target for a wide range of therapeutic small molecules. Thus, deciphering the interaction of drugs with DNA not only offers insights into the rational design of novel and more efficient drugs targeted to DNA, but it also gives an opportunity for developing effective therapeutic agents for the control of gene expression. In the present study, we have attempted to establish the mode of binding of sulindac with calf thymus DNA (Ct-DNA) through various spectroscopic studies and molecular modelling. UV-visible absorbance and steady state fluorescence experiments revealed a binding constant in the order of 103 L mol−1, which is consistent with well-known groove binders. A series of experiments such as competitive displacement assays, iodide quenching, DNA melting study, CD spectral analysis and viscosity measurements were performed to confirm the binding mode. The isothermal calorimetry results were consistent with the groove binders and confirmed that the formation of the sulindac–Ct-DNA complex is spontaneous and enthalpy (ΔH ＜ 0) driven. This is further supported by the consistent results obtained from the in silico molecular docking studies. [ABSTRACT FROM AUTHOR]

Published
2017
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35. Identification of differentially expressed three novel transcript variants of mouse ARNT gene.

Author

Ishqi, Hassan Mubarak, Ur Rehman, Sayeed, Sarwar, Tarique, Husain, Mohammed Amir, and Tabish, Mohammad

Subjects
*ALTERNATIVE RNA splicing, *TRANSCRIPTION factors, *GENE expression, *MESSENGER RNA, *MOLECULAR biology
Abstract

The aryl hydrocarbon receptor nuclear translocator (ARNT/HIF1-β) is an obligatory transcriptional partner of the aryl hydrocarbon receptor (AHR) and hypoxia-inducible factor-1α (HIF-1α). It has a basic helix-loop-helix domain that belongs to period-ARNT-single-minded (PAS) protein family. PAS proteins act as heterodimeric transcription factors with ARNT being master dimerization partner. The ARNT-HIF-1α complex is an important transcriptional regulator of the hypoxic response of the tumor cells. Previous studies have reported two transcript variants of the gene produced by alternative splicing in mouse. One transcript variant contains all 22 exons while the other variant lacks exon-E5. In our study, using combinatorial approach comprising bioinformatics tools and molecular biology techniques involving RT-PCR, semi-nested PCR, sequencing and qPCR, we have identified three novel transcript variants of Arnt gene in mouse. All three new transcripts arise as a result of alternative splicing of newly identified exons with exon-E2, replacing reported exon-E1. These transcripts encode for three protein isofoms having different N-termini. The expression of these transcripts was found to be different in different tissues of adult mice. In silico analysis of the upstream region of the new exons revealed three distinct promoter regions designated as PA, PB and PC present upstream of newly identified exons. These promoters possess potential signature sequences for common as well as different transcription factors suggesting complex regulation of Arnt gene. In silico post translational studies of the conceptually translated amino acid sequences of these transcripts show similarity in some of the properties while differ in others. The diversity at N-termini of protein isoforms suggests the possibility of forming different complexes in different tissues and may also be important for unique interactions with partner molecules. © 2015 IUBMB Life, 68(2):122-135, 2016 [ABSTRACT FROM AUTHOR]

Published
2016
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