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Objectives : This study aimed to evaluate the efficacy and reliability of LC-MS/MS and biochip-based screening in detecting narcotics from blood and urine samples. Materials and Methods : In this single-center study, a total of 250 subjects provided urine and/or blood samples: 234 provided urine and blood samples, and 16 provided only blood samples. Biochip-based narcotics screening was performed on 234 urine and 16 blood samples, and all samples were analyzed using tandem liquid chromatography-mass spectrometry (LC-MS/MS). Results : The two most prevalent narcotics were methamphetamine and amphetamine, with both biochip-based array and LC-MS/MS. Cohen's kappa correlation indicated a substantial agreement between LC-MS/MS and biochip-based screening for both methamphetamine (κ = 0.661, p <0.001) and amphetamine (κ = 0.663, p < 0.001). LC-MS/MS demonstrated near-perfect agreement between urine and blood samples for both methamphetamine (κ = 0.855, p <0.001) and amphetamine (κ = 0.881, p < 0.001). Conclusions : Biochip-based arrays are a valid method offering a faster, less material-intensive alternative to LC-MS/MS for substance detection in forensic examination.

Addiction is a complex, multi-factorial disease, and thus, analyzing genetic variants at multiple loci and gene-gene interactions among them (epistasis) can provide crucial clues about causative factors of addiction which cannot be detected with single-nucleotide polymorphism (SNP) association studies. In this study, we discuss the interaction between the 1359 G/A polymorphism of the CNR1 gene and the DRD2 gene polymorphisms and the net effect of any possible epistasis on the cannabis addiction phenotype in a Turkish population. Using bivariate synergy and mutual information concepts as a means of capturing the magnitude of interaction between marker pairs, the present study not only confirms the A1 marker allele as a risk factor but also reveals a finer-grained association between A and B markers which manifests itself both as a preventive and a risk factor. Our results indicate that the increased phenotype of cases require an individual to be either heterozygous at both loci or homozygous at locus B with homozygous risk factor A1A1 present. We hypothesize that overlapping expressions of CB1 and D2R is the cause of CB1-D2R interactions in cases of substance abuse and the different polymorphisms of CNR1 and DRD2 genes may have decisive roles in the nature of these interactions in terms of promoting or alleviating the cannabis addiction risk factor of the individual.

The gene encoding the dopamine D2 receptor (DRD2) has been suggested as a candidate gene for substance dependence. In this study, the possible association between Taq1A and Taq1B DRD2 polymorphisms and cannabinoid dependence was investigated. One hundred and twelve cannabinoid addicted and 130 healthy control subjects were included in this study. The Taq1A and Taq1B genotypes were determined in all subjects by polymerase chain reaction. For each polymorphism (A or B), the subjects were categorized into three groups according to their genotype, that is, the subjects with alleles A1/A1, A1/A2, A2/A2; B1/B1, B1/B2, and B2/B2. A significant association was found between Taq1A gene polymorphism and cannabinoid addicts compared to the control subjects. This finding suggests that polymorphism of the Taq1A, but not the Taq1B, may be associated with the susceptibility to cannabinoid dependence. Further clinical studies are required to be carried out for confirmation and evaluation of these findings., (© 2012 American Academy of Forensic Sciences.)

Little is known about criminality of cognitively impaired people and also there have been no reports on the relationship between catechol-O-methyl transferase (COMT) and committed Mental Retardation (MR) subjects. In the present study, the association between committed (violent offences) MR subjects and genetic variants of COMT were investigated by using polymerase chain reaction and based restriction fragment length polymorphism methods. During 6 years of follow-up, 36 violent offenders with mild MR were investigated. Thirty-six control volunteers were included in the study as a control group. H/L polymorphism of the COMT gene was investigated in these two groups. In conclusion, the COMT gene genotype distribution and allele frequency is not significantly different between the two groups (p > 0.05). This result suggests that the H/L polymorphism of the COMT gene does not show an association with the potential of "commits-violent offense" of Turkish subjects with mental retardation, compared with control group.

Objectives: The University of Iowa Child Protection Program collaborated with Turkish professionals to develop a training program on child abuse and neglect during 2002-2006 with the goals of increasing professional awareness and number of multidisciplinary teams (MDT), regional collaborations, and assessed cases. This paper summarizes the 5-year outcome., Methods: A team of instructors evaluated needs and held training activities in Turkey annually, and provided consultation when needed. Descriptive analysis was done via Excel and SPSS software., Results: Eighteen training activities were held with 3,570 attendees. Over the study period, the number of MDTs increased from 4 to 14. The MDTs got involved in organizing training activities in their institutions and communities. The number of medical curriculum lectures taught by MDTs to medical students/residents, conferences organized by the MDTs, and lectures to non-medical professional audiences increased significantly (R(2)=91.4%, 83.8%, and 69.2%, respectively). The number of abuse cases assessed by the MDTs increased by five times compared to pre-training period., Conclusions: A culturally competent training program had a positive impact on professional attitudes and behaviors toward recognition and management of child abuse and neglect in Turkey. The need to partner with policy makers to revise current law in favor of a greater human services orientation became clear., Practice Implications: Pioneers in developing countries may benefit from collaborating with culturally competent instructors from countries with more developed child protection systems to develop training programs so that professional development can improve recognition and management of child abuse and neglect.

Family is one of the most important factors in the social and psychological progress of an adolescent. Social behavior disorders in childhood have been reported to be caused by many factors that may lead children to commit a crime. Our study included a total of 106 convicted adolescents from Eastern Turkey in a reformatory and 126 unconvicted adolescents with a similar socio-economic status. A survey form was completed during a face-to-face interview and a review of official records was undertaken. The role of family disruption, education levels of parents, the rate of imprisonment among first- and second-degree relatives, migration as a family from their place of birth, and the number of delinquent children in the family were evaluated. There was a significant difference between the conditions in the families of convicted and unconvicted adolescents. Family factors play an important role in the development of adolescent delinquency. To prevent or decrease this rate of childhood or adolescent delinquency, there needs to be an improvement in the socio-cultural conditions of families.

A wide variety of urethral foreign bodies have been described in the literature. In adults, this is commonly caused by the insertion of objects used for masturbation, and is often associated with a mental disorder as in our case described herein. We report a 28-year-old man, hospitalized for self-insertion of a pen into the urethra. The pen was removed successfully by surgery.