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5 results on '"Isles, A.R."'

1. Developmental disruption to the cortical transcriptome and synaptosome in a model of SETD1A loss-of-function

Author

Clifton, N.E., Bosworth, M.L., Haan, N., Rees, E., Holmans, P.A., Wilkinson, L.S., Isles, A.R., Collins, M.O., and Hall, J.

Abstract

Large-scale genomic studies of schizophrenia implicate genes involved in the epigenetic regulation of transcription by histone methylation and genes encoding components of the synapse. However, the interactions between these pathways in conferring risk to psychiatric illness are unknown. Loss-of-function (LoF) mutations in the gene encoding histone methyltransferase, SETD1A, confer substantial risk to schizophrenia. Among several roles, SETD1A is thought to be involved in the development and function of neuronal circuits. Here, we employed a multi-omics approach to study the effects of heterozygous Setd1a LoF on gene expression and synaptic composition in mouse cortex across five developmental timepoints from embryonic day 14 to postnatal day 70. Using RNA sequencing, we observed that Setd1a LoF resulted in the consistent downregulation of genes enriched for mitochondrial pathways. This effect extended to the synaptosome, in which we found age-specific disruption to both mitochondrial and synaptic proteins. Using large-scale patient genomics data, we observed no enrichment for genetic association with schizophrenia within differentially expressed transcripts or proteins, suggesting they derive from a distinct mechanism of risk from that implicated by genomic studies. This study highlights biological pathways through which SETD1A loss-of-function may confer risk to schizophrenia. Further work is required to determine whether the effects observed in this model reflect human pathology.

Published
2022

4. Maternally derived microduplications at 15q11-q13: implication of imprinted genes in psychotic illness

Author

Ingason, A., Kirov, G., Giegling, I., Hansen, T., Isles, A.R., Jakobsen, K.D., Kristinsson, K.T., Roux, L. le, Gustafsson, O., Craddock, N., Moller, H.J., McQuillin, A., Muglia, P., Cichon, S., Rietschel, M., Ophoff, R.A., Djurovic, S., Andreassen, O.A., Pietilainen, O.P.H., Peltonen, L., Dempster, E., Collier, D.A., St Clair, D., Rasmussen, H.B., Glenthoj, B.Y., Kiemeney, L.A.L.M., Franke, B., Tosato, S., Bonetto, C., Saemundsen, E., Hreidarsson, S.J., Nothen, Markus, Gurling, H., O'Donovan, M.C., Owen, M.J., Sigurdsson, E., Petursson, H., Stefansson, H., Rujescu, D., Stefansson, K., Werge, T., Ingason, A., Kirov, G., Giegling, I., Hansen, T., Isles, A.R., Jakobsen, K.D., Kristinsson, K.T., Roux, L. le, Gustafsson, O., Craddock, N., Moller, H.J., McQuillin, A., Muglia, P., Cichon, S., Rietschel, M., Ophoff, R.A., Djurovic, S., Andreassen, O.A., Pietilainen, O.P.H., Peltonen, L., Dempster, E., Collier, D.A., St Clair, D., Rasmussen, H.B., Glenthoj, B.Y., Kiemeney, L.A.L.M., Franke, B., Tosato, S., Bonetto, C., Saemundsen, E., Hreidarsson, S.J., Nothen, Markus, Gurling, H., O'Donovan, M.C., Owen, M.J., Sigurdsson, E., Petursson, H., Stefansson, H., Rujescu, D., Stefansson, K., and Werge, T.

Abstract

Contains fulltext : 97099.pdf (publisher's version ) (Closed access), OBJECTIVE: Rare copy number variants have been implicated in different neurodevelopmental disorders, with the same copy number variants often increasing risk of more than one of these phenotypes. In a discovery sample of 22 schizophrenia patients with an early onset of illness (10-15 years of age), the authors observed in one patient a maternally derived 15q11-q13 duplication overlapping the Prader-Willi/Angelman syndrome critical region. This prompted investigation of the role of 15q11-q13 duplications in psychotic illness. METHOD: The authors scanned 7,582 patients with schizophrenia or schizoaffective disorder and 41,370 comparison subjects without known psychiatric illness for copy number variants at 15q11-q13 and determined the parental origin of duplications using methylation-sensitive Southern hybridization analysis. RESULTS: Duplications were found in four case patients and five comparison subjects. All four case patients had maternally derived duplications (0.05%), while only three of the five comparison duplications were maternally derived (0.007%), resulting in a significant excess of maternally derived duplications in case patients (odds ratio=7.3). This excess is compatible with earlier observations that risk for psychosis in people with Prader-Willi syndrome caused by maternal uniparental disomy is much higher than in those caused by deletion of the paternal chromosome. CONCLUSIONS: These findings suggest that the presence of two maternal copies of a fragment of chromosome 15q11.2-q13.1 that overlaps with the Prader-Willi/Angelman syndrome critical region may be a rare risk factor for schizophrenia and other psychoses. Given that maternal duplications of this region are among the most consistent cytogenetic observations in autism, the findings provide further support for a shared genetic etiology between autism and psychosis.

Published
2011

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