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11 results on '"Istituto di Genetica Vegetale (IGV)"'

1. Differential expression in a complex poplar hybrid pedigree via RNAseq analysis

Author

Marroni, Fabio, Giorgi, F.M., Pinosio, Sara, Bastien, Catherine, Felice, Nicoletta, Morgante, Michele, Applied Genomics Institute (IGA), Istituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche [Roma] (CNR), Istituto di Genetica Vegetale (IGV), Unité de recherche Amélioration, Génétique et Physiologie Forestières (AGPF), Institut National de la Recherche Agronomique (INRA), Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Udine - University of Udine [Italie], European Project: 211868, Unité de recherche Amélioration, Génétique et Physiologie Forestières (UAGPF), and National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR)

Subjects
descendance, Vegetal Biology, filiation, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, pedigree, Biotechnologies, populus, polymorphisme, RNAseq, identification de gènes, peuplier hybride, heterosis, poplar, pédigrée, hétérosis, vigueur, profil d'expression, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Biologie végétale, expression des gènes
Abstract

absent

Published
2012

2. Comparison of pollen gene flow among four European beech (Fagus sylvatica L.) populations characterized by different management regimes

Author

Koen Kramer, Stefano Leonardi, Giovanni G. Vendramin, Sophie Gerber, Cristina Vettori, Joukje Buiteveld, Thomas Geburek, Andrea Piotti, University of Parma = Università degli studi di Parma [Parme, Italie], Wageningen University and Research Centre (WUR), Federal Research Centre for Forests, Partenaires INRAE, Biodiversité, Gènes & Communautés (BioGeCo), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB), and Istituto di Genetica Vegetale (IGV)

Subjects
0106 biological sciences, CE - Forest Ecosystems, genetic diversity, paternity analysis, neighbourhood model, reproductive success, null alleles, nuclear microsatellites, medicine.disease_cause, 01 natural sciences, Gene flow, Gene Frequency, Fagus, Wageningen Environmental Research, mating patterns, Genetics (clinical), 0303 health sciences, biology, Ecology, Phenology, Reproduction, seed dispersal, sorbus-torminalis, CE - Vegetation and Landscape Ecology, Austria, Seeds, parentage analysis, Pollen, Original Article, France, Gene Flow, DNA, Plant, Genotype, Seed dispersal, pinus-sylvestris l, Forest management, 010603 evolutionary biology, Evolution, Molecular, 03 medical and health sciences, Fagus sylvatica, Genetics, medicine, HETRE COMMUN, Beech, Alleles, 030304 developmental biology, Models, Genetic, Genetic Variation, 15. Life on land, microsatellite markers, silvicultural practices, biology.organism_classification, Genetics, Population, plant-populations, Biological dispersal, habitat fragmentation, Lod Score, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Microsatellite Repeats
Abstract

The study of the dispersal capability of a species can provide essential information for the management and conservation of its genetic variability. Comparison of gene flow rates among populations characterized by different management and evolutionary histories allows one to decipher the role of factors such as isolation and tree density on gene movements. We used two paternity analysis approaches and different strategies to handle the possible presence of genotyping errors to obtain robust estimates of pollen flow in four European beech (Fagus sylvatica L.) populations from Austria and France. In each country one of the two plots is located in an unmanaged forest; the other plots are managed with a shelterwood system and inside a colonization area (in Austria and France, respectively). The two paternity analysis approaches provided almost identical estimates of gene flow. In general, we found high pollen immigration (~75% of pollen from outside), with the exception of the plot from a highly isolated forest remnant (~50%). In the two unmanaged plots, the average within-population pollen dispersal distances (from 80 to 184 m) were higher than previously estimated for beech. From the comparison between the Austrian managed and unmanaged plots, that are only 500 m apart, we found no evidence that either gene flow or reproductive success distributions were significantly altered by forest management. The investigated phenotypic traits (crown area, height, diameter and flowering phenology) were not significantly related with male reproductive success. Shelterwood seems to have an effect on the distribution of within-population pollen dispersal distances. In the managed plot, pollen dispersal distances were shorter, possibly because adult tree density is three-fold (163 versus 57 trees per hectare) with respect to the unmanaged one. © 2012 Macmillan Publishers Limited All rights reserved.

Published
2012
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3. Geography determines genetic relationships between species of mountain pine (Pinus mugo complex) in western Europe

Author

Giovanni G. Vendramin, Santiago C. González-Martínez, Bruno Fady, Myriam Heuertz, Jennifer Teufel, Álvaro Soto, Ricardo Alía, Evolutio nary Biology and Ecology, Université libre de Bruxelles (ULB), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA), Institute for Applied Ecology, University of Canberra, Ecologie des Forêts Méditerranéennes (URFM), Institut National de la Recherche Agronomique (INRA), Istituto di Genetica Vegetale (IGV), Spanish Ministry of Environment CC03-048 AEG06-054, and Spanish National Research Plan REN 2000-1617-GLO

Subjects
0106 biological sciences, Species complex, Historical demography, Range (biology), Post-glacial recolonization, Biología, Genetic clusters, Western Europe, Conservation, 010603 evolutionary biology, 01 natural sciences, PINUS MUGO, 03 medical and health sciences, Sensu, Pinus mugo, Silvicultura, Hybridization, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, 0303 health sciences, Genetic diversity, Ecology, biology, Scots pine, Pinus sylvestris, 15. Life on land, biology.organism_classification, Haplotype sharing, Phylogeography, Pinus mugo complex, Genetic structure, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Chloroplast microsatellites
Abstract

Aim Our aims were to test whether morphological species of mountain pines were genetically supported in the western part of the distribution range of the Pinus mugo species complex (Pinus mugo Turra sensu lato), to resolve genetically homogeneous clusters of populations, to determine historical demographic processes, and to assess the potential hybridization of mountain pines with Scots pine, Pinus sylvestris L. Location Populations were sampled in the Iberian System, the Pyrenees, the French Mont Ventoux, Vosges and Jura mountains, the German Black Forest and throughout the Alps. This corresponded to a range-wide sampling for mountain pine sensu stricto (Pinus uncinata Ram.) and to a sampling of the western parts of the ranges of dwarf mountain pine (Pinus mugo Turra sensu stricto) and bog pine/peatbog pine [Pinus rotundata Link/Pinus × pseudopumilio (Willk.) Beck]. Methods In total, 786 individuals of P. mugo sensu lato from 29 natural populations, and 85 individuals of P. sylvestris from four natural populations were genotyped at three chloroplast microsatellites (cpSSRs). Populations were characterized for standard genetic diversity statistics and signs of demographic expansion. Genetic structure was explored using analysis of molecular variance, differentiation statistics and Bayesian analysis of population structure (BAPS). Results One hundred haplotypes were identified in P. mugo sensu lato. There was a stronger differentiation between geographical regions than between morphologically identified taxa (P. mugo sensu stricto, P. uncinata and P. rotundata/P. ×pseudopumilio). Overall genetic differentiation was weak (GST = 0.070) and displayed a clear phylogeographic structure [NST = 0.263, NST > N ST (permuted), P < 0.001]. BAPS identified a Pyrenean and an Alpine gene pool, along with several smaller genetic clusters corresponding to peripheral populations. Main conclusions The core regions of the Pyrenees and Alps were probably recolonized, respectively by P. uncinata and P. uncinata/P. mugo sensu stricto, from multiple glacial refugia that were well connected by pollen flow within the mountain chains. Pinus rotundata/P. × pseudopumilio populations from the Black Forest, Vosges and Jura mountains were probably recolonized from various glacial populations that kept their genetic distinctiveness despite late glacial and early Holocene expansion. Marginal P. uncinata populations from the Iberian System are compatible with elevational shifts and long-term isolation. The causes of haplotype sharing between P. mugo sensu lato and P. sylvestris require further research. © 2009 Blackwell Publishing Ltd.

Published
2010
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4. Effects of seed dispersal, adult tree and seedling density on the spatial genetic structure of regeneration at fine temporal and spatial scales

Author

Bruno Fady, Christian Pichot, Giovanni G. Vendramin, Fabrice Sagnard, Sylvie Oddou-Muratorio, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Ecologie des Forêts Méditerranéennes (URFM), Institut National de la Recherche Agronomique (INRA), and Istituto di Genetica Vegetale (IGV)

Subjects
0106 biological sciences, SPATIAL DISTRIBUTION, NATURAL REGENERATION, F40 - Écologie végétale, Seed dispersal, SAPIN ARGENTE, Population, Horticulture, Spatial distribution, 010603 evolutionary biology, 01 natural sciences, F30 - Génétique et amélioration des plantes, 03 medical and health sciences, [SDV.SA.SF]Life Sciences [q-bio]/Agricultural sciences/Silviculture, forestry, stomatognathic system, GENETIC MARKER, Genetics, SPATIAL GENETIC STRUCTURE, DISPERSION DES SEMENCES, education, Molecular Biology, 030304 developmental biology, 0303 health sciences, education.field_of_study, STRUCTURE DU GENOME, biology, Ecology, U10 - Informatique, mathématiques et statistiques, Temperate forest, food and beverages, Forestry, 15. Life on land, Régénération naturelle, biology.organism_classification, Abies alba, SEED DISPERSAL, Seedling, Biological dispersal, Gap dynamics, POLLEN DISPERSAL, Seed dispersal . Spatial genetic structure . Density. Demography
Abstract

International audience; Several demographic factors can produce family structured patches within natural plant populations, particularly limited seed and pollen dispersal and small effective density. In this paper, we used computer simulations to examine how seed dispersal, density, and spatial distribution of adult trees and seedlings can explain the spatial genetic structure (SGS) of natural regeneration after a single reproductive event in a small population. We then illustrated the results of our simulations using genetic (isozymes and chloroplast microsatellites) and demographic experimental data from an Abies alba (silver fir) intensive study plot located in the Southern French Alps (Mont Ventoux). Simulations showed that the structuring effect of limited dispersal on seedling SGS can largely be counterbalanced by high effective density or a clumped spatial distribution of adult trees. In addition, the clumping of natural regeneration far from adult trees, which is common in temperate forest communities where gap dynamics are predominant, further decreases SGS intensity. Contrary to our simulation results, low adult tree density, aggregated spatial distribution of seedlings, and limited seed dispersal did not generate a significant SGS in our A. alba experimental plot. Although some level of long distance pollen and seed flow could explain this lack of SGS, our experimental data confirm the role of spatial aggregation (both in adult trees and in seedlings far from adult trees) in reducing SGS in natural populations

Published
2010
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5. Putative glacial refugia of Cedrus atlantica from Quaternary pollen records and modern genetic diversity

Author

Jean-Pierre Suc, Kaizhu Huang, Rachid Cheddadi, Giovanni G. Vendramin, Louis François, Lara Hajar, Elena Ortu, Bruno Fady, Maxime Demarteau, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Ecologie des Forêts Méditerranéennes (URFM), Institut National de la Recherche Agronomique (INRA), Département d'astrophysique, Géophysique et Océanographie (AGO), Université de Liège, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Sun Yat-Sen University [Guangzhou] (SYSU), Istituto di Genetica Vegetale (IGV), Centre Interdisciplinaire Scientifique de la Montagne (CISM), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Unité de Recherches Forestières Méditerranéennes (URFM), Laboratoire de Physique Atmosphérique et Planétaire (LPAP), PaleoEnvironnements et PaleobioSphere (PEPS), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Fujitsu Research and Development Center Ltd, Fujitsu, Instituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche (CNR), Laboratoire de chrono-écologie (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Environnements, Dynamiques et Territoires de la Montagne (EDYTEM), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Laboratoire de chrono-écologie - CNRS (UMR6565) (LCE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), and Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])

Subjects
0106 biological sciences, 010504 meteorology & atmospheric sciences, Biogeography, [SDE.MCG]Environmental Sciences/Global Changes, [SDV]Life Sciences [q-bio], Cedrus atlantica, Climate change, phylogeography, medicine.disease_cause, 010603 evolutionary biology, 01 natural sciences, Cedrus, Pollen, medicine, GLACIAL REFUGIA, Glacial period, Ecology, Evolution, Behavior and Systematics, Holocene, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, Ecology, biology, NORTHERN AFRICA, PHILOGEOGRAPHY, VEGETATION MODEL, Cedar, DNA, 15. Life on land, biology.organism_classification, vegetation model, Geography, 13. Climate action, GENETIC DIVERSITY, Quaternary
Abstract

International audience; Aim To investigate the impact of past environmental changes on Cedrus atlantica and its current genetic diversity, and to predict its future distribution. Location Morocco, Algeria and Tunisia. Methods Eleven fossil pollen records from these three countries were used to locate putative glacial refugia and to reconstruct past climate changes. A mechanistic vegetation distribution model was used to simulate the distribution of C. atlantica in the year 2100. In addition, a genetic survey was carried out on modern Moroccan C. atlantica. Results Pollen records indicate that Cedrus was present during the last glacial period, probably in scattered refugia, in Tunisia, Algeria and Morocco. In the Tunisian and Algerian sites, cedar expanded during the late glacial and the early Holocene, then disappeared after c. 8000 yr bp. Reconstructed mean annual precipitation and January temperature show that the last glacial period in Morocco was cooler by 10–15C and drier by c. 300–400 mm year)1 than the climate today. Modern chloroplast microsatellites of 15 C. atlantica populations in Morocco confirm the presence of multiple refugia and indicate that cedar recolonized the Moroccan mountains fairly recently. Model simulation indicates that by the year 2100 the potential distribution of C. atlantica will be much restricted with a potential survival area located in the High Atlas. Main conclusions Environmental changes in northern Africa since the last glacial period have had an impact on the geographical distribution of C. atlantica and on its modern genetic diversity. It is possible that by the end of this century C. atlantica may be unable to survive in its present-day locations. To preserve the species, we suggest that seedlings from modern C. atlantica populations located in the High Atlas mountains, where a high genetic diversity is found, be transplanted into the western High Atlas.

Published
2009
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6. An Italian functional genomic resource for Medicago truncatula

Author

Francesco Panara, Sergio Arcioni, Carla Scotti, Paola Taviani, Gérard Duc, Viviane Cosson, Pascal Ratet, Luisa Lanfaloni, Henri de Larembergue, Andrea Porceddu, Efisio Piano, Laura Scaramelli, Lorna Molinari, Gianluca Bruschi, Ornella Calderini, Maria Carelli, Istituto di Genetica Vegetale (IGV), Università degli Studi di Sassari, Università degli Studi di Perugia (UNIPG), Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, Centre National de la Recherche Scientifique (CNRS), UMR 0102 - Unité de Recherche Génétique et Ecophysiologie des Légumineuses, Génétique et Ecophysiologie des Légumineuses à Graines (UMRLEG) (UMR 102), and Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
0106 biological sciences, TILLING, medicago truncatula, legumes, mutant collection, tnt1 mutagenesis, tilling, Resource (biology), [SDV]Life Sciences [q-bio], Mutagenesis (molecular biology technique), lcsh:Medicine, Computational biology, Biology, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, lcsh:Science (General), lcsh:QH301-705.5, 030304 developmental biology, Medicine(all), 0303 health sciences, Biochemistry, Genetics and Molecular Biology(all), business.industry, AGR/07 Genetica agraria, fungi, lcsh:R, food and beverages, General Medicine, biology.organism_classification, Medicago truncatula, Reverse genetics, Biotechnology, lcsh:Biology (General), [SDE]Environmental Sciences, Project Note, business, Functional genomics, 010606 plant biology & botany, lcsh:Q1-390
Abstract

Background Medicago truncatula is a model species for legumes. Its functional genomics have been considerably boosted in recent years due to initiatives based both in Europe and US. Collections of mutants are becoming increasingly available and this will help unravel the genetic control of important traits for many species of legumes. Findings Our report is on the production of three complementary mutant collections of the model species Medicago truncatula produced in Italy in the frame of a national genomic initiative. Well established strategies were used: Tnt1 mutagenesis, TILLING and activation tagging. Both forward and reverse genetics screenings proved the efficiency of the mutagenesis approaches adopted, enabling the isolation of interesting mutants which are in course of characterization. We anticipate that the reported collections will be complementary to the recently established functional genomics tools developed for Medicago truncatula both in Europe and in the United States.

Published
2008
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7. Insertion mutagenesis in Medicago truncatula. The grain legume (FP6-GLIP) Tnt1 project

Author

Ratet, Pascal, Angenon, Geert, Arcioni, Sergio, Atanassov, Atanas, Barascud, Marielle, Barker, David, Brocard, Lysiane, Caballero, Teresa, Calderini, Ornella, Cayrel, Anne, Chabaud, Mireille, Cosson, Viviane, del Cerro, Carolina, Den Herder, Griet, Donkova, Patia, Endre, Gabriela, Ferrandiz, Cristina, Goormachtig, Sofie, Holster, Marcelle, Lantcheva, Anelia, Jacas, Louis, Kiss, Ernő, Krastanova, Kety, Madueno, Francisco, Ochatt, Sergio, Panara, Francesco, Porceduu, Andrea, Primard-Brisset, Catherine, Rakocevic, Alexandra, Schultze, Michael, Schultz, Bernadette, Thompson, Richard, Thu, Tran Thanh, Tiritz, Hilda, Vaughan, David, Verplancke, Christa, Vlahova, Mariana, Unité mixte de recherche interactions plantes-microorganismes, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Vrije Universiteit Brussel (VUB), Istituto di Genetica Vegetale (IGV), AgroBioInstitute, Bordeaux Imaging Center (BIC), Université de Bordeaux (UB)-Institut François Magendie-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Centre National de la Recherche Scientifique (CNRS), Departement of Plant Systems Biology, Flanders Institute for Biotechnology, Hungarian Academy of Sciences (MTA), Universiteit Gent = Ghent University [Belgium] (UGENT), UMR 0102 - Unité de Recherche Génétique et Ecophysiologie des Légumineuses, Génétique et Ecophysiologie des Légumineuses à Graines (UMRLEG) (UMR 102), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and University of York

Subjects
Tnt1 retransposon, [SDV]Life Sciences [q-bio], Medicago truncatula, international collaboration, GLIP, T-DNA, [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, insertion de mutants, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2007

8. Eggplant genetic resources network (EGGNET))

Author

Daunay, M-Christin, Hennart, J.W., van der Weerden, G., Barendse, G., Haanstra, J., Lester, R.N., van Dooijeweert, W., Prohens, J., Bletsos, F., Polignano, G., Börner, A., Peron, J.Y., Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de la Recherche Agronomique (INRA), Vilmorin, Radboud university [Nijmegen], Rijk Zwaan Seed, Partenaires INRAE, University of Birmingham, Wageningen University and Research Centre (WUR), Universitat Politècnica de València (UPV), Aristotle University of Thessaloniki, Istituto di Genetica Vegetale (IGV), Leibniz Institute of Plant Genetics and Crop Plant Research, and Institut National d'Horticulture

Subjects
RESOURCES CONSERVATION, DATABASE, [SDV]Life Sciences [q-bio], GRAFT, DISEASE RESISTANCE, FRUIT QUALITY, CORE COLLECTION, BASE DE DONNEES, ComputingMilieux_MISCELLANEOUS, SOLANUM
Abstract

International audience

Published
2004

9. Breeding for a new generation of Prunus rootstocks based on marker-assited selection : a European initiative

Author

M. Di Vito, Jean-Luc Poëssel, Roger Voisin, J. Gómez-Aparisi, Werner Howad, Pere Arús, M. Claverie, E. Dirlewanger, N. Bosselut, M. Kleinhentz, F. Laigret, Bartolomeo Dichio, Daniel Esmenjaud, María José Rubio-Cabetas, Cristos Xiloyannis, Unité de recherches Espèces Fruitières et Vigne (UREFV), Institut National de la Recherche Agronomique (INRA), Centro de Investigacion y Tecnologia Agroalimentaria de Aragon (CITA), Interactions plantes-microorganismes et santé végétale (IPMSV), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Universidad de, Génétique et Amélioration des Fruits et Légumes (GAFL), Istituto di Genetica Vegetale (IGV), and Institute of Agrifood Research and Technology (IRTA)

Subjects
0106 biological sciences, [SDV]Life Sciences [q-bio], 04 agricultural and veterinary sciences, AMELIORATION DES PLANTES, Horticulture, Marker-assisted selection, Biology, 01 natural sciences, Prunus, Agronomy, PRUNIER, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, SELECTION ASSISTEE PAR MARQUEUR, Rootstock, ComputingMilieux_MISCELLANEOUS, 010606 plant biology & botany
Abstract

National audience

Published
2003

10. Sorghum, a healthy and gluten-free food for celiac patients as demonstrated by genome, biochemical, and immunochemical analyses.

Author

Pontieri P, Mamone G, De Caro S, Tuinstra MR, Roemer E, Okot J, De Vita P, Ficco DB, Alifano P, Pignone D, Massardo DR, and Del Giudice L

Subjects
Celiac Disease metabolism, Diet, Gluten-Free, Humans, Male, Plant Proteins administration & dosage, Plant Proteins chemistry, Plant Proteins genetics, Sorghum genetics, Sorghum immunology, Celiac Disease diet therapy, Food, Organic analysis, Genome, Plant, Sorghum chemistry, Sorghum metabolism
Abstract

Wheat (Triticum spp. L.), rye (Secale cereal L.), and barley (Hordeum vulgare L.) seeds contain peptides toxic to celiac patients. Maize (Zea mays L.) and rice (Oryza sativa L.) are distant relatives of wheat as well as sorghum (Sorghum bicolor (L.) Moench) and are known to be safe for celiacs. Both immunochemical studies and in vitro and in vivo challenge of wheat-free sorghum food products support this conclusion, although molecular evidence is missing. The goal of the present study was to provide biochemical and genetic evidence that sorghum is safe for celiac patients. In silico analysis of the recently published sorghum genome predicts that sorghum does not contain peptides that are toxic for celiac patients. Aqueous/alcohol-soluble prolamins (kafirins) from different sorghum varieties, including pure lines and hybrids, were evaluated by SDS-PAGE and HPLC analyses as well as an established enzyme-linked immunosorbent assay (ELISA) based on the R5 antibody. These analyses provide molecular evidence for the absence of toxic gliadin-like peptides in sorghum, confirming that sorghum can be definitively considered safe for consumption by people with celiac disease.

Published
2013
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11. Pectin methylesterase in Citrus bergamia R.: purification, biochemical characterisation and sequence of the exon related to the enzyme active site.

Author

Laratta B, De Masi L, Minasi P, and Giovane A

Abstract

Three forms of pectin methylesterase (PME) were purified, from bergamot fruit (Citrus bergamia R.), to homogeneity by ion-exchange and affinity chromatography. The isoforms, named PME I, PME II and PME III, according their elution order on a heparin-sepharose column, were characterized for their relative molecular mass, activity kinetic parameters and thermostability. The molecular mass was estimated to be 42kDa for the three forms, and the apparent Km values for citrus pectin were 0.9mg/ml for PME I and 0.5mg/ml for PME II and PME III. The optimum pH values lie within the range 6.5-9.0, depending on salt concentration. Thermal behaviours of the three PME isoforms were studied in a temperature range from 65°C to 80°C with the less abundant PME I isoform showing a higher heat resistance. Moreover, the complete exon 2 sequence of PME gene was acquired (GenBank accession no. DQ458770) using a PCR-based approach on well-known Citrus genomic DNA present in the NCBI database., (Copyright © 2008 Elsevier Ltd. All rights reserved.)

Published
2008
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