1. Tspan33 is Expressed in Transitional and Memory B Cells, but is not Responsible for High ADAM10 Expression
- Author
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Albert Zlotnik, Leopoldo Santos-Argumedo, Iván Meza-Herrera, César Augusto Pérez-Martínez, José L. Maravillas-Montero, and Felipe Vences-Catalán
- Subjects
0301 basic medicine ,Antigens, Differentiation, T-Lymphocyte ,Time Factors ,Tetraspanins ,Immunology ,Cell ,Naive B cell ,Antigens, CD19 ,Gene Expression ,Biology ,Lymphocyte Activation ,Flow cytometry ,03 medical and health sciences ,ADAM10 Protein ,Antigen ,Tetraspanin ,Antigens, CD ,Cell Line, Tumor ,medicine ,Humans ,Lectins, C-Type ,B cell ,Cells, Cultured ,B-Lymphocytes ,medicine.diagnostic_test ,Reverse Transcriptase Polymerase Chain Reaction ,General Medicine ,Flow Cytometry ,Transmembrane protein ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Cell culture ,Immunologic Memory - Abstract
Tetraspanins are a family of transmembrane proteins that form membrane microdomains. They play important roles in migration, adhesion and other cellular processes. TspanC8, a subfamily of tetraspanins, was found to associate and promote ADAM10 trafficking and cell surface localization. One of its members, Tspan33, is expressed in activated B cells. Using RT-PCR and flow cytometry, we analysed the pattern of expression of Tspan33 in B cells from healthy donors. We found Tspan33 expression in early and late stages of B cell development. However, Tspan33 expression did not correlate with ADAM10 surface expression. We also found expression of Tspan33 early in the activation process. Given its predominant expression in activated B cells and in several lymphomas, but not in naive B cells, we hypothesize that Tspan33 could be a potential target for therapeutic purposes.
- Published
- 2016